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Title:
2-KETO-L-GULONIC ACID PRODUCTION STRAIN AND CONSTRUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/036788
Kind Code:
A1
Abstract:
Provided is a construction method for a 2-keto-L-gulonic acid production strain, comprising one or more of the following steps in any order: 1) interrupting a membrane-bound glucose dehydrogenase encoding gene in the chromosome of Tatumella; 2) interrupting a glucose kinase encoding gene; 3) interrupting a gluconate kinase encoding gene; 4) interrupting a 2-ketose or aldose reductase encoding gene and/or an isozyme encoding gene thereof; 5) recombinantly expressing an NAD(P)+ type glucose dehydrogenase; 6) recombinantly expressing an NAD(P)H type 2,5-diketo-D-gluconate reductase; 7) overexpressing 2,5-diketo-D-gluconate endo-transporters ARU92990 and ARU92994; and 8) interrupting a PTS component encoding gene in the chromosome of a cell to obtain genetically engineered bacteria. The 2-keto-L-gulonic acid production bacteria constructed by said method can produce 2-keto-L-gulonic acid by using glucose as the raw material by using a one-step fermentation process or whole cell catalysis.

Inventors:
YANG SHENG (CN)
WANG QINGZHUO (CN)
DUAN CHUNLAN (CN)
LIU YINGMIAO (CN)
DIAO LIUYANG (CN)
JIANG YU (CN)
TAO RONGSHENG (CN)
Application Number:
PCT/CN2020/108609
Publication Date:
March 04, 2021
Filing Date:
August 12, 2020
Export Citation:
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Assignee:
CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES (CN)
International Classes:
C12N1/21; C12P7/60; C12R1/185
Foreign References:
CN1500148A2004-05-26
US20050227337A12005-10-13
Other References:
DUAN, CHUNLAN: "One-step fermentation of glucose to produce vitamin C precursor 2-keto-l-gulonic acid", MASTER DISSERTATION, 31 December 2016 (2016-12-31), pages 1 - 80, XP009526322
Attorney, Agent or Firm:
SUNHOLD LAW FIRM (CN)
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