Title:
ACTIVE-SITE ENGINEERING OF NUCLEOTIDYLTRANSFERASES AND ENZYMATIC METHODS FOR THE SYNTHESIS OF NATURAL AND "UNNATURAL" UDP- AND NUCLEOTIDE SUGARS
Document Type and Number:
WIPO Patent Application WO2002048331
Kind Code:
A3
Abstract:
The present invention provides mutant nucleotidylyltransferases, such as E>p<, having altered substrate specificity; methods for their production; and methods of producing nucleotide sugars, which utilize these nucleotidylyltransferases. The present invention also provides methods of synthesizing desired nucleotide sugars using natural and/or modified Ep or other nucleotidyltransferases; and nucleotide surgars sythesized by the present methods. The present invention further provides new glycosyl phosphates, and methods for making them.
Inventors:
THORSON JON S (US)
NIKILOV DIMITAR B (US)
NIKILOV DIMITAR B (US)
Application Number:
PCT/US2001/047953
Publication Date:
July 10, 2003
Filing Date:
December 13, 2001
Export Citation:
Assignee:
SLOAN KETTERING INST CANCER (US)
THORSON JON S (US)
NIKILOV DIMITAR B (US)
THORSON JON S (US)
NIKILOV DIMITAR B (US)
International Classes:
C07H11/04; C07H15/24; C07H15/252; C07H17/00; C07H17/08; C07H19/10; C07H19/207; C12N9/12; C07B61/00; (IPC1-7): C12N9/12; C07H15/24; C07H17/00; C07H17/08; C07H19/207; C07H11/04; C07H15/252; C07H19/10
Other References:
LINDQUIST LENNART ET AL: "Purification, characterization and HPLC assay of Salmonella glucose-1-phosphate thymidylyltransferase from the cloned rfbA gene.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 211, no. 3, 1993, pages 763 - 770, XP001118907, ISSN: 0014-2956
SOLENBERG P J ET AL: "PRODUCTION OF HYBRID GLYCOPEPTIDE ANTIBIOTICS IN VITRO AND IN STREPTOMYCES TOYOCAENSIS", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 4, no. 3, 1997, pages 195 - 202, XP008007222, ISSN: 1074-5521
BÜLTER T ET AL: "Enzymatic synthesis of nucleotide sugars.", GLYCOCONJUGATE JOURNAL. UNITED STATES FEB 1999, vol. 16, no. 2, February 1999 (1999-02-01), pages 147 - 159, XP009004001, ISSN: 0282-0080
HUTCHINSON C R: "COMBINATORIAL BIOSYNTHESIS FOR NEW DRUG DISCOVERY", CURRENT OPINION IN MICROBIOLOGY, CURRENT BIOLOGY LTD, GB, vol. 1, no. 3, 1998, pages 319 - 329, XP000915705, ISSN: 1369-5274
BARTON W A ET AL: "STRUCTURE, MECHANISM AND ENGINEERING OF A NUCLEOTIDYLYLTRANSFERASE AS A FIRST STEP TOWARD GLYCORANDOMIZATION", NATURE STRUCTURAL BIOLOGY, NEW YORK, NY, US, vol. 8, no. 6, June 2001 (2001-06-01), pages 545 - 551, XP001098651, ISSN: 1072-8368
SOLENBERG P J ET AL: "PRODUCTION OF HYBRID GLYCOPEPTIDE ANTIBIOTICS IN VITRO AND IN STREPTOMYCES TOYOCAENSIS", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 4, no. 3, 1997, pages 195 - 202, XP008007222, ISSN: 1074-5521
BÜLTER T ET AL: "Enzymatic synthesis of nucleotide sugars.", GLYCOCONJUGATE JOURNAL. UNITED STATES FEB 1999, vol. 16, no. 2, February 1999 (1999-02-01), pages 147 - 159, XP009004001, ISSN: 0282-0080
HUTCHINSON C R: "COMBINATORIAL BIOSYNTHESIS FOR NEW DRUG DISCOVERY", CURRENT OPINION IN MICROBIOLOGY, CURRENT BIOLOGY LTD, GB, vol. 1, no. 3, 1998, pages 319 - 329, XP000915705, ISSN: 1369-5274
BARTON W A ET AL: "STRUCTURE, MECHANISM AND ENGINEERING OF A NUCLEOTIDYLYLTRANSFERASE AS A FIRST STEP TOWARD GLYCORANDOMIZATION", NATURE STRUCTURAL BIOLOGY, NEW YORK, NY, US, vol. 8, no. 6, June 2001 (2001-06-01), pages 545 - 551, XP001098651, ISSN: 1072-8368
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