Title:
AGAR-DIGESTING ENZYME AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2004/090127
Kind Code:
A1
Abstract:
An agar-digesting enzyme having the following properties. (1) Function: Hydrolyzing β-1,4 bond of agarose to form neoagarooligosaccharides. (2) Substrate specificity: Acting at least on polysaccharides having a skeleton in which D-galactose units and 3,6-anhydro-L-galactose units are bonded to each other alternately via β-1,4 bond and &agr -1,3 bond (for example, agar and agarose) and oligosaccharides having the above skeleton to form agar-origin oligosaccharides. (3) Effect of temperature: Sustaining its activity even after heating to 54°C or higher for 30 minutes. Because of having a high activity at a high temperature compared with the existing agar-digesting enzymes, this enzyme can elevate the digestion speed of agar and thus is advantageously usable for industrial purposes.
Inventors:
OHTA YUKARI (JP)
HATADA YUJI (JP)
NOGI YUICHI (JP)
ITO SUSUMU (JP)
HORIKOSHI KOKI (JP)
HATADA YUJI (JP)
NOGI YUICHI (JP)
ITO SUSUMU (JP)
HORIKOSHI KOKI (JP)
Application Number:
PCT/JP2004/004128
Publication Date:
October 21, 2004
Filing Date:
March 24, 2004
Export Citation:
Assignee:
INDEPENDENT ADMINISTRATIVE INSTITUTION JAPAN AGENCY FOR MARINE EARTH SCI & TECH (JP)
OHTA YUKARI (JP)
HATADA YUJI (JP)
NOGI YUICHI (JP)
ITO SUSUMU (JP)
HORIKOSHI KOKI (JP)
OHTA YUKARI (JP)
HATADA YUJI (JP)
NOGI YUICHI (JP)
ITO SUSUMU (JP)
HORIKOSHI KOKI (JP)
International Classes:
C12N1/15; C12N1/19; C12N1/21; C12N9/38; C12N15/56; C12P19/00; (IPC1-7): C12N9/42; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/56; C12P19/12; C12P19/14; C12Q1/68
Foreign References:
| JPH05252947A | 1993-10-05 |
Other References:
SUGANO Y. ET AL: "Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. Strain JT0107", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 5, 1993, pages 1549 - 1554, XP002980367
Attorney, Agent or Firm:
Ono, Nobuo (1-13-1 Kandaizumi-ch, Chiyoda-ku Tokyo, JP)
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