Background art (WO 92/231017) discloses compounds of formulae in which formulae I-III:

RI is independently a substituted or unsubstituted C7-30 straight chain or cyclic compound; R2 is independently a substituted or unsubstvtuted Cl-C30 straight chain or cyclic compound which may be internally interrupted by O, NH, NR3 or S, where R3 is a C1-CR alkyl; n is 0-1 in formula I and 1-20 in formula III; m is 1-2; B is a substituted or unsubstituted C1-C30 straight chain or cyclic compound which may be internally interrupted by C, NH, NR3 or S.

In formula IV: Rif R2 are independently H or a substituted or unsubstituted C7- C30 straight chain or cyclic compound; R3, R4 are independently H or a substituted or unsubstituted C1- C30 straight chain or cyclic compound which may be internally interrupted by 0, NH, NR5 or S, where R5 is a C alkyl ; and in formula V: R1 is independently a substituted or unsubstituted C7-C30 straight chain or cyclic compound R2 is independently a substituted or unsubstituted C1-C30 straight chain or cyclic compound which may be internally interrupted by 0, NH, NR4 or S, where R4 is a C1-C3 alkyl; R3 is independently a substituted or unsubstituted C1-C30 straight chain or cyclic compound which may be internally interrupted by 0, NH, NR or S, where R4 is a C1-C3 alkyl and m is 0-12.

The reference also discloses contrast agents obtained with the compounds of the above formulae (I-V), the latter further comprising lipids; the lipids are in the form of emulsions, liposomes or micelles.

US-A-5,312,617 dicloses a method of imaging comprising administering to patients a contrast agent comprising a complex of a paramagnetic metal and a ligand selected from formulae IV and V disclosed in the foregoing US-A-5,466, 38.

Liposomes incorporating the above chelates are also disclosed as well as the possibility of having the compounds in the form of emulsions or micelles.

The micelles can be prepared by ã variety C~ conventional liposome preparatory techniques; suitable lipids include, for example, monomyristoyl-phosphaeicyl-choline, monopalmitoyl-phosphatidylcholine, dibutyroyl-phosphatidylcholine and the like, linoleic acid, oleic acid, palmitic acid, and the like.

Lipid emulsions can be prepared by conventional techniques, for instance a typical method is as follows: 1. In a suitable flask, the lipids are dissolved in ethanol or chloroform or any other suitable organic solvent.

2. The solvent is evaporated leaving a thin layer of lipid at the bottom of the flask.

3. The lipids are resuspended in an aqueous medium, such as phosphate buffered saline, this producing an emulsion 4. Sonication or microfluidization can then be applied to improve homogeneity.

5. The contrast agents can be added to the lipids during preparation of the emulsion, or they may be added to the emulsion afterwards.

6. Useful additives include, for example, soybean lecithin, glucose, Pluronic F-68 and D, L-a-tocopherol; these additives are particularly useful where injectable intravenous formulations are desired.

The foregoing contrast agents may further comprise suspension stabilizers such as polyethyleneglycol, lactose, mannitol, sorbitol, ethyl alcohol, glycerin, lecithin, polyoxyethylene sorbitan monoleate, sorbitan monoleate and albumin. Various sugars and other polymers may also be added, such as polyethylene glycol, polyvinylpyrrolidone, polypropylene glycol and polyoxyethylene.

The contrast agents of this reference have high Tu and T2 relaxivity, especially when lipids are also present. Because of the high relaxivity, these contrast media are particularly useful for imaging the blood pool.

Summary of the invention Despite the merit of the paramagnetic polycarboxylic chelates of the prior art as contrast agents for MRI, there was a need for a new range of chelating compounds of further improved properties designed to provide blood-pool contrast agents of outstanding long life in the circulation. In view of their structure including strongly hydrophobic and hvdrophilic moities, the compounds of the present invention achieve a significant step in the right direction.

The novei compounds of the present invention, either racemic or enantiomeric, have the following formulae (#) and (IV)

in which n and m are 1 or 0 but not simultaneously 1, and when n = m = 0, R'is H, and R* is a C12-25 linear or ramified, saturated or unsaturated, hydrocarbon radical; when n = 1 and m = 0, R* is H or a CI-3 alkyl or alkylene substituent; and R'is selected from-NHR3,-NR4R5 and-OR6 where the R3 to R6 are independently Cl-25 linear or ramified, saturated or unsaturated, hydrocarbon radicals optionally interrupted by-CO- and/or-O-and optionally terminated by-NR7Rg in which R7 and R8 are independently H or C12-25 hydrocarbon radicals; when n = 0 and m = 1, R* is H or a C1-3 alkyl or alkylene substituent; and R'is selected from Rg and-CH2-O-CO-Rg in which Rg is a C10-30 linear or ramified, saturated or unsaturated, hydrocarbon radical optionally interrupted by-NH-,-NRlo-,-CO-or-O-, Rlo being a lower aliphatic hydrocarbon; and R12 is H or a Ci2_30 hydrocarbon radical optionally interrupted by-NH-,-NR1g-,-CO-or-O-and optionally terminated by a cholesteryl residue, and the R13 are-OH; or one or two R13 are a-NH- R14 group in which R14 is a C2-30 linear or ramified, saturated or unsaturated, hydrocarbon radical optionally interrupted by-NH-,- NR10-,-CO-,-O-, and/or-OPO (OH) O-, the remaining R13 being-OH.

The compounds of formulae (t) and (IV) can be used as chelates of paramagnetic metals, preferably Gd (III), Mn (II), Cr (III), Cu (II), Fe (III), Pr (III), Nd (III), Sm (III), Tb (III), Yb (III), Dy (III), Ho (III) and Er (III) in the preparation of MRI contrast formulations and compositions of outstanding long life in the blood which makes them ideal agents for investigating the circulation in appended organs.

Detailed description of the invention Preferred are the compounds encompassed by formula (t), in which n and m are 1 or 0 but not simultaneously 1, and when n = 1 and m = 0, R* is an alkylene group, and the other variable groups are the meanings defined above.

Equally preferred are the compounds encompassed by formula (t), in which n and m are 1 or 0 but not simultaneously 1, and when n = 1 and m = 0, R* is H or a Cl-3 alkyl; and R'is selected from-NHR3, or-NR4R5, where the R3 to R5 groups are independently C12-25 linear or ramified, saturated or unsaturated, hydrocarbon radicals, optionally interrupted by -CO-and/or-O-and optionally terminated by-NR7Rg in which R7 and R8 are independently H or C12-25 hydrocarbon radicals with the same meaning just above defined.

Equally preferred are the compounds encompassed by formula (t), in which n and m are 1 or 0 but not simultaneously 1, and when n = 0 and m = 1, R* is an alkylene group, and the other variable groups are the meaning defined above.

Equally preferred are the compounds encompassed by formula (t), in which n and m are 1 or 0 but not simultaneously 1, and when n = 1 and m = 0, R* is H or a C1-3 alkyl; and R'is selected from Rg and-CH2-O-CO-Rg in which Rg is a C12-25 linear or ramified, saturated or unsaturated, hydrocarbon radical optionally interrupted by-NH-,-NRlo-,-CO-or-0-, Rlo being a C1_4 linear or ramified, saturated or unsaturated, hydrocarbon radical.

Furthermore are prefered the compounds of general formula (IV) in which R12 is H, and two R13 are a-NH-Rl4 group in which R14 is a C12_ 25 linear or ramified, saturated or unsaturated, hydrocarbon radical optionally interrupted by-NH-,-NRlo-,-CO-,-0-, and/or-OPO (OH) O-, the remaining R13 being-OH.

The compounds of formula (t) are preferably selected among compounds of the following formulae (I), (II) or (III)

wherein R* is as defined heretofore; R is H or a C1_3 alkyl or alkylene substituent; R1 is selected from-NHR3,-NR4RS and-OR6 where the R3 to R6 are independently C1-25 linear or ramified, saturated or unsaturated, hydrocarbon radicals optionally interrupted by-CO-and/or-O-and optionally terminated by-NR7Rg in which R7 and R8 are independently H or C12-25 hydrocarbon radicals; R2 is selected from Rg and-CH2-0-CO-R9 in which Rg is a C10-60 linear or ramified, saturated or unsaturated, hydrocarbon radical optionally interrupted by one or more-NH-,-NRlo-,-CO-or-0-, R1o being a lower aliphatic hydrocarbon.

For instance, the compounds of formula (II) can have formula (IIa) in which R3 is a C12-2s linear or ramified, saturated or unsaturated, hydrocarbon radical.

Particularly preferred are the compounds of formula (IIa) in which R3 is a C16-20 linear or ramified, saturated or unsaturated, hydrocarbon radical.

Or they can have formula (IIb)

in which R4 and R5 are independently C12-25 linear or ramified, saturated or unsaturated, hydrocarbon radicals optionally interrupted by-CO-and/or-O-.

Particularly preferred are the compounds of formula (IIb) in which R4 and R5 are independently C16-20 linear or ramified, saturated or unsaturated, hydrocarbon radicals interrupted by-CO-and-O-.

Furthermore, they can have formula (IIc) in which A is-NH-or-0-, A1 is a Cl-20 linear or ramified, saturated or unsaturated, hydrocarbon radicals optionally interrupted by-CO-and/or-O-and R7 and Rg are defined as above.

Particularly preferred are the compounds of formula (IIc) in which R is an alkylene substituent.

Equally preferred are the compounds of formula (IIc) in which A is-NH-, A1 is a Cl-20 linear or ramified, saturated or unsaturated, hydrocarbon radicals interrupted by-CO-and-0-and R7 and R8 are defined as above. Also the compounds of formula (IIc) are preferred in which A is-O-and A1 is a Ci-20 linear or ramified, saturated or unsaturated, hydrocarbon radicals interrupted by-CO-and-0-and R7 and R8 are defined as above.

Compounds of formula (III) can have formula (IIIa) in which R is H and R2 is is a C10-30 linear or ramified, saturated or unsaturated, hydrocarbcn radical optionally interrupted by one or more-NH-,-N-,-CO-or-0. Otherwise, they can have formula below

in which R is H and Rg is a C10-25 linear alkyl, or a C10-50 linear or ramified, saturated or unsaturated, hydrocarbon radical optionally interrupted by one or more-N-,-CO-and/or-O-.

Some preferred compounds of formula (IV) are those in which all the R13 are-OH and R12 is defined as mentioned above. Otherwise, compounds of formula (IV) can be selected from the compounds of formulae (IVa) and (IVb) below. In Formula (IVa), the R14 are independently as defined above for formula (IVj.

Particularly preferred are the compounds of formula (Iva) in which R14 is C12-25 linear or ramified, saturated or unsaturated, hydrocarbon radical optionally interrupted by-CO-and/or-O-, and/or- OPO (OH) O-.

In formula (IVb) shown below, R14 is a C12-25 linear or ramified, saturated or unsaturated, hydrocarbon radical.

The compounds of this invention of formulae (t) and (IV) can be as represented in the formulae, or they can be in the form of complex chelates with paramagnetic metal ions (as indicated heretofore) and the salts thereof with physiologically acceptable bases selected from primary, secondary, tertiary amines and basic aminoacids, or inorganic hydroxides of sodium, potassium, magnesium, calcium or mixtures thereof; or with physiologically acceptable anions of organic acids selected from acetate, succinate, citrate, fumarate, maleate, oxalate, or inorganic acids selected from hydrogen halides, sulphates, phosphates, phosphonates and the like; or with cations or anions of aminoacids selected from lysine, arginine, ornithine, aspartic and glutamic acids, and the like; For preparing the compounds of formula (I) in the form of complexes with metals (ME), one can proceed as in the following Scheme 1: Scheme 1 PgOOC/\ COOPg HOOC A COOH OOC A coo- EN N N N COMPLEXATION N N . R-X N N 2. DEPROTECTION N N SALIFICATION N N PgOOC~ \ H HOOC \ R OOC~ \ | R b1 1A""1C Where Pg is a protecting group; R* is as defined for formula (I) ; ME is a metal ion ; n = 2 or 3.

In step al the compound 1A, i. e. 1,4,7,10-tetraazacyclododecane- 1,4,7-triacetic acid, whose carboxylic groups are suitably protected by groups such as benzyl or t-butyl, is reacted with R*-X, where R* is a substituent residue and X is a leaving group such as C1, Br, I. The reaction product is then deprotected by known methods (e. g. with CF3COOH) to give the free ligand 13. The ligand is then complexed with a suitable metal ion oxide or salt (preferably paramagnetic), such as Gd oxide, chloride or acetate, in order to obtain the desired metal complex chelate 1C. Depending on the value of n, 1C may be salified with a suitable counter-ion.

For the complexes of the compounds of formula (IIa), one may proceed according to the scheme 2 below: Scheme 2 PgOOC-\/\/-COOPg HOOC-\/--\/-COOH'OOC-/--\/-COO' 1. 2C. HEAT _ C N N COMPLEXATION C N N N N 2 DEPROTECTION N N//SALIFICATION N N// SALIFICATION PgOOC-\J H HOOC- OOC-\J y R R- 2D 2E 2F

in which Pg is a protecting group as in Scheme 1; R and R3 have been defined above; and ME and n are as in Scheme 1.

According to the method of Scheme 2, one prepares-ompound 2C by the reaction of a halogenated halide (or equivalent)'.'.'th a primary amine R3NH2 in a suitable solvent, such as CH2C12, CHC13 or H2O/CH2C12 mixtures, in the presnce of a base (e. g. K2CO3). Then, Cc : npound 2C is reacted with compound 2D and the product is deprotected (b2) to furnish the desired free ligand 2E. The latter is fina'ly complexed according to the general procedure disclosed in Scheme 1. If required, i. e. depending on whether n has an appropriate value, compound 2F may be salified with a suitable counter-ion.

For preparing the metal complexes of the compounds of formula (IIb), one may proceed according to the Scheme 3 below: Scheme 3 PgOOC-\/\/-COOPg pgooc-\//-COOPg DEPROTECTION 3C. PPAA C N N N N ET3N C N N 2 c3 PgOOC~ COOH b3 PgOOC~ \J ~t (CH2) n (0CH2CH2) pOOCAlk N 3D3E (CH2) n (0CH2CH2) pOOCAlk 3D 3E HOOC-\ A COOH OOC A coo- N N-COOHCOMPLEXATION/SALIFICATION B d3 C Mem+ O HOOC N 2), (OCH2CH2), OOCAlk-ooc (CH2), (OCH2CH2) POOCAlk N--N N (CH2), (OCH2CH2) POOCAlk (CH2) n (OCH2CH2) pOOCAlk 3F 3G

in which n = 1-6; p = 0-5; m = 2 or 3; Y = halogen; Pg s a protecting group and Alk is a lipophilic alkyl chain.

In step a3 compound 3A is reacted with a suitable long-chain carboxylic acid halide 3B in a suitable aprotic dipolar solvent to obtain compound 3C. The latter is reacted (step b3) with compound 3D, i. e. 1,4, 7, 10-tetraaza-cyclododecane-1, 4, 7,10-tetraacetic acid of which three acetic groups are protected with, for example, benzyl or t-butyl groups, in the presence of 1-propanephosphonic acid cyclic anhydride (PPAA) and a base (e. g. Et3N) in a suitable solvent such as CH2C12.

Compound 3E obtained in step c3 is deprotected by known methods (e. g. by catalytic hydrogenation) which provides the free ligand 3F, which is then complexed with a metal (step d3), according to the procedure described earlier. This affords the desired complex chelate 3G. Then, compound 3G may be salified with a suitable counter-ion if the value of m permits.

For preparing the compounds of formula (Iic) in which A is-NH-, one can proceed like in the previous scheme, the first step (condensation of triprotected 1,4,7,10-tetraazacyclododecane-1,4,7,10- tetraacetic acid (4A) with an amine H2N-A1-NR7R8 (4B) being effected in the presence, as condensing a agent, of (benzotriazo-1-yloxy)- tris (dimethylamino)-phosphonium hexafluorophosphate and a sterically hindered tertiary amine such as diisopropylethylamine (DIEA) when 4B is an a-aminoacid derivative, or N. N'-bis (2-oxo-3-oxazolidyl)- phosphoro-diamidic chloride (BOP). This is illustrated in Scheme 4.

Scheme 4 PgOOC-\/\/-COOPg PgOOC-\/\/-COOPg S N N) HCI H2N-A1-NR7R8 W4B) C b4 N N CONDENSATION (A4) N N 0 b4 PgOOC ~ \/COOH PgOOC ~ L/~ (HN-A-NR7Ra (4B) 4A 4A 4C HOOC/\ COOH OOC/\ COOENN COMPLEXATION/SALIFICATION N N N N C N N : : , N /N N,// HN-A,-NR, R,'--'HN-A,-NR, R, 4D 4E

A similar method can be applied for compounds (IIc) in which A is -0-, i. e. the esterification of triprotected 1,4,7,10- tetraazacyclododecane-1,4,7,10-tetraacetic acid with a halogenated compound X-A1-NR7Rg _n the presence of 1,8-dlazabicyclo [5.4.0] undec- ene. Then the resulting intermediate 5C is deprotected, complexed and salified as in the previous schemes (Scheme 5).

Scheme 5 PgOOC-COOPgPgOOC-s./\/-COOPg DEPROTECTION ZONN X-At-NR7R8 (5B) N N 4A 4C sua, 5) 4A 4C O-A-NRRB 4A 4C Hooc- n i-oaH-ooc n --oo N N COMPLEXATION/SALIFICATION N N D 0 c5 Mem'D 0 N N N N HOOC HOOC- OOC U p-A-NRRe 5D SE 5D5E The compounds of formula (IIIa) can be obtained as illustrated in Scheme 6. The protected 1, 7-triacetic acid 6A is reacted with an alkyl epoxide in a solvent such as ethanol and the resulting product is deprotected and treated as described in the previous schemes Scheme 6 PgOOC/\.A cooPg o FUN NX/\ R (6B) + Heat, then (H+) P9OC- N-COOPg N N OH PgOOC ~ \/H a6 b6 N N/ PgOOC- R2 R2 6A 6C 6C COMPLEXATION/SALIFICATION Onc COO Con N N ou cl -ooc-- U R "2 6D

A possible technique for making the compounds (IIIb) and the corresponding metal chelates and salts is illustrated in Scheme 7.

Here the initial reactant to be condensed with the triprotected 1,4,7,10-tetraaza-cyclododecane-1,4,7-triaacetic acid is 2,3- epoxypropanol 7B which provides the vic-diol 7C, the primary-OH of which is thereafter esterified with an acid Rg-COOH, the remaining steps being as described previously.

Scheme 7 HOOC o PgOOC-\/--\-COOPg CN N R9-COOH, DEPROTECT. OH N N heat N N b7 PgOOC- \/H PgOOC- \/\--\, OH 7A 7C 7A 7C HOOC/\gCOOHN N COMPLEXATION/SALIFICATION 4 N N N OH C Me+ N N OH HOOC U R9-Ooc---/,_j--O \ R9 0 in 0 !' 7D 7E 0 The following preparative methods are applicable regarding the compounds of formula (IV) in which R12 is H. For instance, for the compounds (IVa), one may operate as illustrated in Scheme 8, by reacting in DMF the DTPA cyclic dianhydride (N, N-bis 2- (2,6-dioxo-4- morpholinyl) ethyl] gly-cine) 8A with an amine H2NR14, the remaining step being that of complexation with a metal and possible salification as discussed earlier.

Scheme 8 COOH COOH H2N-Chain 00-HOOC N N COOH a8 Chain-NH-CO CO-NH-Chain 8A 8C COOO COMPLEXATION/SALIFICATION OOC^NNN^COO b8 b8 Chain-NH-CO n+ CO-NH-Chain 8D

Otherwise, one may first effect protection of up to four c ; the- COOH's groups in DTPA, the unprotected group being thereafter amidated in DMF with an amine H2NRI4 according to usual means (see Scheme 9.

Scheme 9 C COOH C CONHR Condens. with H2NR4/N PgOOC N N COOPg p9ppC ^ N I N COOPg PgOOCcoopg Pgooc coopg C CONHR14 C CONHR14 N N COMPLEXATION/SALIFICATION HOOC N N COOH OOC N N COO HOOC COOH'OOC COO One preparative route for the compounds of formula (IV) with a substituent R12 in a-to the central carboxylic function is to first attach in the said position, a carbon chain functionalized with, for example, a-NH2 or-COOH group (Scheme 10). For example, compound 10A can be prepared, according to Rapoport et al. in J. Org. Chem. 58 (1993), 1151-1158, or using a method disclosed in WO 98/05626. The synthon is then reacted with, for example, a chloride of a carboxylic acid having the desired chain iength, or with a suitable amine, depending on the nature of the said functional group. Then, the resulting compound 10B is deprotected and complexed as already shown in the previous Schemes.

Scheme 10 Functionalized subst. COOH Y R2'COOH RCOCI OR R'NH2,etc. PgOOC^NNN^COOPg PgOOC PgOOCCOOPg PgOOC J COOPg 9 10A 10B R,COO DEPROTECTION COMPLEXATION/SALIFICATION ^ N OOC N N COO ! ooc. coo 10C

This techniaue is exemplified in the synthesis of the compound of Example 16 involving reaction with cholesteryl chloroformate.

The injectable compositions and formulations according to the invention which are usable as contrast agents for MRI investigations will preferably contain further additives, in addition 10 one or more of the afore discussed novel paramagnetic chelates and a carrier liquid. The additives include non-ionic and/or ionic surfactants and mixtures thereof, as well as other amphipatic compounds. Due to their physiological suitability, the non-ionic surfactants are preferred.

The non-ionic surfactants are preferably block-copclymers having polyoxyethylene and polyoxypropylene sequences, polyethyleneglycol- alkylethers such as, for example, polyethyleneglycol-cctadecylether, or polyoxyethylene fatty acid esters or polyoxyethylene sorbitan fatty acid esters, or n-alkyl glycopyranoside and n-alkyl maltotrioside. The non-ionic surfactant in the compositions of theinventionis conveniently selected from the commercially available croducts, such as Pluronic, Poloxamer, Poloxamine, Synperonic, bRIJ, Myrj, <BR> <BR> <BR> @<BR> Tween s (polysorbates) and their mixtures. The weight proportion of the surfactant relative to the amount of the paramagnetic imaging agent is from 1: 50 to 50: 1, preferably 1: 10 to 10: 1, and even more preferably 1: 1. The ionic surfactants preferably include biliary acid salts such as sodium deoxycholate.

The amphipatic compounds suitable in the present compositions are phospholipids which may be selected from phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylglyceroi (PG), phosphatidylinositol (PI), cardiolipin (CL) and sphyngomielin (SM).

The amphipatic compound may also consists of a monophosphate ester of a substituted or partially substituted glycerol, at least one functional group of said glycerol being esterified by saturated or unsaturated aliphatic fatty acid, or etherified by saturated or unsaturated alcohcl, the other two acidic functions of the phosphoric acid being either iree or salified with alkali or earth-alkali metals.

Preferably the phosphate esters will include monophosphaies of fatty acid glycerides selected from dimiristoylphosphai. idic acid, dipalmitoylphosphatidic acid, or distearoylphosphatidic acid.

The phospholipids may also include diacyl and dialkyl glycerophospholipids in'..'hich the aliphatic chains have at least

twelve carbon wellasoneormorecompoundsselectedfromas ionic and neutral phosDhoiipids, monoaikyl or alkenyi esters of phosphoric acid and/cr cholesterol, ergosterol, phytosterol, sitosterol, lanosterol, tocophercl. In the compositions containing phospholipids, the weight proportion of the phospholipids to the amphiphilic chelate seems not critical and it may vary, f-example, from 1: 50 to 50: 1. The practical range will be between 10: 1 and 1: 10, preferably between 1: 5 and 5: 1 and even more preferably between 1: 3 and 3: 1. In the compositions in which phospholipids are used the weight ratio of the phospholipid to the surfactant may vary as above, however the ranges from 1: 10 to 10: 1 and, preferably, between 1: 2 and 2: 1 are considered optimal.

The compositions of the present invention may exist in micellar form, in which case they can be prepared using known techniques, namely as described in WO 97/00087; Polym. Prepr. 1997,38 (1), 545- 546; Acad. Radiol. 1996,3,232-238. These documents describe micelles of amphiphilic Gd chelates useful in percutaneous lymphography. The micelles have particle size between i0 and 500 nm, preferably between 50 and 200 nm.

The micelles can be prepared in any physiologically acceptable aqueous liquid carrier, such as water or saline, neat or bufferd, according to usual practice. Depending upon the choice of the components, the dispersion can be achieved by gentle mixing or by more energetic means, such as homogenisation, microfluidization or sonication.

In an advantageous mode of preparing the micelles cf the invention, one part by weight of the paramagnetic chelate contrast component is admixed with one to two parts each of surfactants and of lipids, and with 100 to 200 parts of liquid carrier, for example Tris/Glycerol buffer.

The compositions can be stored and used as such, or may be lyophilized dry, according to known methods, e. g. by freeze-drying.

This dry form (porous lumps or free flowing powder) is particularly convenient for long-ter storage. The formulations can be reconstituted before usage by dispersion of the lyophilizate in a physiologically acceptable liquid carrier, thus obtaining a suspension corresponding to the eariy formulation and directly usable as NMR imaging contrast agent.

For practically applying the compositions of the invention in the medical field, the lyophilized components and the carrier liquid can be marketed separately in a kit form. The _yophilized components may be stored under a dry, inert atmosphere ar. d the carrier liquid may further contain isotonic additives ar. c other physiologically acceptable ingredients, such as various mineral salts, vitamines, etc.

The compositions of the invention are particularly useful as magnetic resonance contrast agents for the imaging of the blood pool.

They have shown to possess a sufficiently high relaxivity effect on the blood after injection in the rat and an exceptionally favourable elimination kinetic profile from the blood circulation, as demonstrated by pharmacokinetic and biodistribution data. These two combined characteristics make them very suitable for angiographic magnetic resonance imaging in general. The compositions of the invention can therefore facilitate MR angicgraphy and help to assess myocardial and cerebral ischemia, pulmonary embolism, vascularization of tumours and tumour perfusion.

The following examples further illustrate the invention in more detail.

Example 1 10-Hexadecyl-1,4,7,10-tetraazacyclododecane-1,4,7-triacetato (3- )gadolinium A) 10-Hexadecyl-1,4,7,10-tetraazacvclododecane-1,4,7-triacetic acid A mixture of 10-tetraazacyclododecane-1,4,7-triacetic acid tris (1, 1-dimethylethyl) ester (prepared according to EP-A-299795) (20.6 g; 40 mmol) and 1-bromohexadecane (12.4 g; 40.6 mmol) in CH3CN (500 mL) was heated to reflux for 2 h. Then, the reaction mixture was evaporated and the residue was flash chromatographed (CH2Cl2/MeOH = 9/1 (v/v) to give a solid. This product was dissolved in CHC13 and an excess CF3COOH was added. After 2 h the reaction mixture was evaporated and the oily residue redissolved in CF3COOH. After 16 h at room temperature the solution was evaporated and the residue was purified by flash chromatography (CH2Cl2/MeOH/NHdOH 25 (w/w) = 12/4/1

(viv/v)). The product was dissolvedinHOanaacicif-eawith6wHC1; the solution was loaded onto an AmberliteL XAD-8 resin colt-. n and eluted with a CH3CN/H2O gradient.

The fractions containing the product were evaporated and dried under reduced pressure to give the desired product (c. l g; 1'mmol ;.

Yield 35%. HPLC: 98% (area 2.). Karl Fisher (K. F.):4.05.The'C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): I c H N Calcd. 63. 13 10. 24 9. 82 Found 63.16 10. 53 9. 84 anhydrous B) 10-Hexadecvl-1,4,7,10-tetraazacyclododecane-1,4,7- triacetato (3-)] gadolinium A solution of GdCl3#6H2O (3.9 g; 10.5 mmol) in H2O (40 mL) was added to a solution of the product from the previous preparation (6 g; 10.5 mmol) in H2O ; the pH was maintained at 6.8 by addition of a solution of NaOH 1N (30 mL). The solution was treated with n-BuOH and the organic phase was evaporated to give a solid that was extracted at reflux with CHC13 in a Soxhlet apparatus. The solution was evaporated to give a solid. The product was dissolved in H2O and i-PrOH, ioaded onto a mixed bed of AmberliteD IRA 400 (250 mL) and Colites C20 MB resin (250 mL); then, it was and eluted with H2O/i-PrOH 1: _. The fractions containing the product were evaporated to give the title compound (2 g; 2.7 mmol). Yield 26%.

HPLC: 99% (area %); K. F. : Weight loss: (120°C): 10.45%.

The MS and IR spectra were consistent with the structure postulated.

Elemental analysis (%): NGdCH Calcd. 7.7321.697.65 7.95Found49.80 7.69 21.59 EXAMPLE 2 10-Octadecyl-1,4,7,10-tetraazacvclododecane-1, 4, 7-triacetato (3- )] gadolinium

A) 10-Octadecyl-1,4,7,10-tetraazacyclododecane-l,4,7-triacetic acid A mixture of 1,4,7,10-tetraazacyclododecane-1, 4, 7--riacetic acid tris (1, 1-dimethylethyl) ester (37.5 g; 72.8 mmol) and 1- bromooctadecane (24.5 g; 73.5 mmol) in CH3CN (500 mL) was heated to reflux. After 2 h the reaction mixture was evaporated and the residue was dissolved in CHC13 and a portion of CF3COOH was added. After 16 h at room temperature the reaction mixture was evaporated and the oily residue dissolved in CF3COOH. After 3 days at room temperature, the solution was evaporated, the residue taken up in CHCl3 and the solution evaporated. This operation was repeated three times. The oily residue was purified by flash chromatography as follows: Eluents: (a) CH2Cl2/MeOH = 3/1 (v/v) 3 litres (b) CH2Cl2/MeOH/NH4OH 25% (w/w) = 12/4/1 (v/v/v) 12 litres (c) CH2Cl2/MeOH/NH4OH 25% (w/w) = 6/3/1 (v/v/v) 2 litres The product was dissolved in H2O and acidified with 6N HC1; then, the solution was loaded onto an Amberlite# XAD-8 resin column and eluted with a CH3CN/H2O gradient. The product started eluting with 20% CH3CN.

The fractions containing the product were evaporated and dried under reduced pressure to give the desired product (24.2 g; 40.4 mmol). Yield 55%.

HPLC: 91% (area %); K. F. : the C-NMR, MS and d IR spectra were consistent with the structure.

Elemental analysis (%): C H N 10.449.36Calcd.64.18 Found 4. 17 10. 48 9. anhydrous

B) 10-Octadecyl-1,4,7,10-tetraazacyclododecane-1,4,7- triacetato (3-)] gadolinium (CH3COO)3Gd (7.05a;I".3mmol) was added to a suspension of the free ligand issued from the previous preparation (10.4 g; 17. 3 mmol) in MeOH (400 mL) at 50°C. ' The reaction mixture was kept at 50°C for 1 h, after which the clear solution was evaporated and dried under reduced pressure to give the title compound (11 g; 14.6 mmol). Yield 84%. HPLC : 100% (area %); K. F. : 1. 83% ; Weight loss (120°C) : 5.04%.

The MS and IR spectra were consistent with the structure.

Elemental analysis (%) :

EXAMPLE 3 10-(2-Hydroxyoctadecyl)-1, 4,7,10-tetraazacyclo-dodecane-1,4,7- triacetato- (3-) gadolinium

A) 10-(2-Hydroxvcctadecyl)-1, 4, 7, 10-tetraazacvclodode-cane- 1,4,7-triacetic acid A solution of 1,2-epoxyoctadecane (17.4 g; 65 mmol) in abs. EtOH (100 mL) was added dropwise to a solution of 1,4,7,10- acid tris-(1, 1-dimethylethyl) ester (33.3 g; 65 mmol) in abs. EtOH and the mixture was heated to reflux. After 3 h, the reaction mixture was evaporated and the residue was dissolved in EtOAc and washed with brine. The organic phase was separated, dried over Na2SO4 and evaporated under reduced pressure.

The residue was fiash-chromatographed, (Eluent: CH2Cl2/MeOH = 9/1 (v/v)) The obtained product was dissolved in SN HCl (500 mL) and the solution was heated to reflux. After 1.5 h, the mixture was evaporated

and the residue purified by flash c.-romatography CHsCl/MeOH/NHOH 25% (w/w) = 12/4/1 (v/v/v)) The product was dissolved in H20 and 6N HCl, the solution was loaded onto an Amberlitee XAD-8 res'n columr. (800 mL) and eluted with a gradient.

The fractions containing the product were evaporated and dried under reduced pressure to give the desired product (18 g; 29 mmol).

Yield 45 %. Acidic titer (0.1 N NaOH): 95 % HPLC: 94% (area %); K. F. : 7.10%. The C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): I c H N Calcd. 62.51 10. 16 9.11 Found 62.93 10. 26 9. 14 anhydrous B) 10- (2-Hydroxyoctadecyl)-1,4,7,10-tetraazacyclodode-cane- 1,4,7-triacetato- (3-) gadolinium Gd203 (3.62 g.; 10 mmol) was added to a solution of the free ligand issued from the previous preparation (12.3 g; 20 mmol) in H2O (150 mL) and the resulting suspension was heated at 50°C for 40 h.

The. reaction mixture was filtered through a Millipore# apparatus (HA 0.45 Hm filter); the filtrate (pH 6.7) was evaporated under reduced pressure and dried to give the title compound (14.2 g.; 18 mmol).

Yield 92%. Free ligand : 0.7%; HPLC : 95 % (area %); K. F. : 7.94 %.

The MS and IR spectra were consistent with the structure.

Elemental analysis (%): NGdCH 7.737.2820.45Calcd.49.97 7.887.2920.57Found49.98 |anhydrous EXAMPLE 4 10- 2- (Octadecylamino)-2-oxoethyl-1,4,7,10-tetraazacyclo- dodecane-1,4,7-triacetato (3-) gadolinium A) 2-Bromo-N-octadecylacetamide (C. A. S. registrv number 15491-43- 7)

A solution of bromoacetyl bromide (44.4 g; 0.22 mol) n CH2Cl2 (50 mL) was added dropwise in 2. 5 h at 20°C to a mixture of octadecylamine (59.3 g ; 0.22 mol) and K2CO3 (30.4 g; 0.22 mol) in CH2Cl2 After 16 h at room temperature the organic layer was separated, washed with H2O, dried over Na2SOq and evaporated. The crude product was purified by flash chromatography (CH2Cl2/MeOH = 100/1 (v/v)) to give the desired product (60 g; 0. 154 mol). Yield 70%. GC : 96% (area %) K. F. : <0.1%; 1H-NMR, 13C-NMR and MS spectra were consistent with the postulated structure.

Elemental analysis (%): NBrOCH Calcd. 61.52 10.33 3.59 20.46 4.09 10.713.5820.144.01Found61.75 B) 10- [2-(Octadecvlamino)-2-oxoethyl]-1, 4,7,10-tetra- azacyclododecane-1,4,7-triacetic acid A mixture of 7-triacetic acid tris (1,1-dimethylethyl) ester (24 g; 46.6 mmol) and 2-bromo-N- octadecylacetamide (18.2 g; 46.6 mmol) in EtOH (500 mL) was heated to reflux. After 2.5 h, the reaction mixture was evaporated, the residue was dissolved in CH2CI2 and CF3COOH was added. After 15 min, the solvent was evaporated and the oily residue dissolved in CF3COOH.

After 16 h at room temperature the solution was evaporated and the oily residue was purified by flash chromatography (CH2Cl2/MeOH = 3/1 (v/v); then CH2Cl2/MeOH/NHqOH 25% (w/w) = 12/4/1 (v/v/v)).

The product was dissolved in H2O and 6N HC1, the solution was loaded onto an Amberlite XAD-8 resin column and eluted with a CH3CN/H20 gradient. The product elutes with 50% CH3CN.

The fractions containing the product were evaporated and dried under reduced pressure to give the desired product (12 g; 18 mmol).

Yield 39%. Acidic titer (0.1 N NaOH): 91%; HPLC: 95% (area %); K. F.: 8.82%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elementalanalysis C H N Calcd. 10.689.99 Found 62. 289. 6310.64 anhydrous C) 10-2-(Octadecylamino)-2-oxoethyl-1, 4, 7, 10-tetra- azacyclododecane-1,4,7-triacetato! 3-)) gadolinium

Gd203 (1. 97 g; 5.4 mmol) was added to a solut-cr. of the free ligand from the previous preparation (7.12 g; 9.7 mmc) in H2O (310 mL) and the resulting suspension was heated to 50 C or 9.5 h. The reaction mixture was filtered through a Millipore me-rane (HA 0.45 µm filter) and the solution was evaporated to give the title compound (8.6 g; 9.5 mmol). Yield 98%. HPLC: 98% (area %); K. F.: MS and IR spectra were consistent with the structure.

Elemental analysis (%): NGdCH 7.718.6419.41Calcd.50.41 Found 8.6519.327.78 anhydrous EXAMPLE 5 [10-[2-(Dioctadecylamino)-2-oxoethyl]-1,4,7,10-tetraazacyclo do- decane-1,9, 7-triacetato (3-)) gadolinium

A) 2-Bromo-N, N-dioctadecylacetamide This novel compound was prepared as follows: Bromoacetyl bromide (4.25 g; 21 mmol) was added dropwise to a solution of dicctadecylamine (10 g; 19 mmol) and Et3N (2.13 g; 21 mmol) in CHC13 (400 mL). After 4 h at room temperature the reaction solution was washed with HOOC dried over Na2SO4 and evaporated. The residue was purified by flash chromatography (n-hexane/Et2O = 8/2 (v/v) to give the desired product (7.5 g; 11.5 mmol). Yield 61%. K. F.: <0.1%; The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the postulated structure Elemental analysis : C H N Br Calcd. 70.99 11. 91 2. 18 12. 43 12.182.1112.27Found71.05 B) 10-[2-(Dioctadecylamino)-2-oxoethyl)-1, 4,7,10-tetraazacyclo- dodecane-1,4,7-triacetic acid trihvdrochloride

A mixture of 1,4,7,10-tetraazacyclododecane-l, 4, 7-triacetic acid tris (1, 1-dimethylethyl) ester (14.4 g; 28 mmol) and 2-bromo-N, N- dioctadecylamide (18.1 g; 28 mmol) in EtOH (800 mL) was heated to reflux. After 3 h the reaction mixture was evaporated and the residue was dissolved in CH2Cl2. The solution was washed with brine, dried over Na2SO4 and evaporated to give the crude alkylated ester. This product was suspended in 5N HCl and refluxed. After 2 h the suspension was filtered, the solid was washed with 5N HCl and dried under reduced pressure to give the desired compound (21. 5 g; 21 mmol). Yield 75%.

HPLC: 95.7 % (area %). Argentometric titer (0.1 N AgN03): K. F.: 4.79%. The H-NMR, 13C-NMR, MS and IR spectra were consistent with the desired structure.

Elemental analysis (%): CHClN : Calcd. 61.7 10. 29 10. 45 6.88 Found 61. 30 10. 08 10. 71 6. 44 anhydrous C) 10- 2- (Dioctadecylamino)-2-oxoethyl-1,4,7,10-tetraaza- (3-) gadolinium A solution of GdCl3 6H20 (5.8 g; 15.7 mmol) in H20 (50 mL) was added dropwise to a refluxing solution of the product from the previous preparation (16 g; 15.7 mmol) and 1 N NaOH (94.3 mL; 94.3 mmol) in abs. EtOH (1 L). After 1.5 h the mixture was cooled to room temperature, filtered and concentrated to half its volume, thus causing the precipitation of a solid which was filtered, washed with H20 and dried under reduced pressure. The solid was purified by flash chromatography (CH2Cl2/MeOH/Et3N = 16/4/1 (v/v/v)) and then suspended in H2O at 50°C for 3 h. The suspension was drained and the solid was washed with H2O and dried under reduced pressure to give the title compound (11.5 g; 10.8 mmol). Yield 69%. HPLC: 96% (area %); K. F. : 3.93%. The MS and IR spectra were consistent with the structure.

Elemental analysis (%): I c H I N I Gd_ Calcd. 58. 78 9.30 6.59 14. 80 Found 58.59 9. 14 6. 36 14.26 anhydrous

EXAMPLE 6 10-f2-Hydroxy-3-(1-oxooctadecyl)oxypropyl-1,4,7,10-terraaza- cyclododecane-1,4, 7-triacetato (3-)] gadolinium A) 10-(2, 3-Dihydroxypropyl)-1,(2, 3-Dihydroxypropyl)-1, 4,7,10-tetraazacvclodo-decane- 1,4,7-triacetic acid tris dimethvlethyl) ester adduct with NaCl A solution of 2,3-epoxypropanol (3.7 g; 50 mmol) in abs. EtOH (80 mL) was added dropwise in 30 min to a refluxing solution of 10- tetraazacyclododecane-1,4,7-triacetic acid tris (1,1-d-methylethyl) ester (25.7 g; 50 mmol) in abs. EtOH (250 mL). After 2.5 h the solution was evaporated, the residue taken up with EtOAc and washed with brine. The organic phase was separated, dried (Na2SO4) and evaporated. The crude was purified by flash chromatography (CH2Cl2/MeOH = 9/1 (v/v)) to give the desired compound (24 g; 37 mmol). Yield 74%. K. F.: The C-NMR, MS and IR spectra were consistent with the desired structure.

Elemental analysis (%): C I H I N C1 I Na Calcd. 53.82 8. 72 8. 66 5. 48 3.55 Found 53. 95 8. 87 8. 72 5. 47 3. 48 anhydrous B) 10-2-Hydroxy-3-(1-oxooctadecyl) oxy propyl-1, 4, 7,10-tetra- azacyclododecane-1,4,7-triacetic acid tris- (1, 1-dimethylethyl) ester adduct with NaCl A solution of dicyclohexylcarbodiimide (0.43 g; 2.1 mmol) in CHC13 (10 mL) was added dropwise in 10 min to a solution of stearic acid (0.44 g; 1.5 mmol) (commercial product), 10- (2,3- dihydroxypropyl)-1,4,7,10-tetraazacyclo-dodecane-1,4,7-triac etic acid tris (1,1-dimethylethyl) ester adduct with NaCl (1 g; 1. 5 mmol) and 4- (dimethylamino) pyridine (0. 06 g; 0.5 mmol) (commercial product) in CHC13 (40 mL) at 0°C. The temperature of the reaction mixture was allowed to come back to normal. After 16 h, the mixture was concentrated to half its volume, the precipitate was filtered off and the solution evaporated. The crude was purified by flash

chromatography (CH2Cl2/MeOH = 10/1 (v/v)) to give the desired cempound (0.95 g; 1.04 mmol). Yield 67%. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the structure.

C) 10-2-Hydroxy-3-(1-oxooctadecyl)cxypropyi-1,4,7,10-etra- azacyclo-dodecane-1,4,7-triacetato (3-)] gadolinium CF3COOH (20 mL) was added to a solution of the product from the previous preparation (13.9 g; 15.2 mmol) in CH2C12 (5 mL). After 24 h the solution was evaporated and the residue taken up with more CF3COOH (15 mL). After 6 h the mixture was evaporated and the crude desalted by dialysis (Spectra/PorO CE (Cellulose Ester) membrane MWCO 500) to afford a white solid (4 g). A portion of this solid (2.9g, was dissolved in 2-propanol (100 mL) and H20 (25 mL) at 80°-then (CH3COO) 3Gd (1.7 g) was added and the solution kept at 80°C for 3 h.

The reaction mixture was evaporated and the residue was purified by flash chromatography (CH2Cl2/MeOH/H2O = 5/5/1 (v/v/v)) to gi-.-e the title compound (1.53 g; 1.8 mmol). Yield 12%. HPLC : 100% (area%); Weight loss (120°C) : 4. 96%. The MS and IR spectra were consistent with the postulated structure.

Elemental analysis (%): C H N Gd Calcd. 49.98 7. 55 6. 66 18. 69 Found 50.24 7. 63 6.61 18. 39

EXAMPLE 7 [10-[2-Hydroxy-3-[[[2-(Octadecyloxy)-1-[(octadecyloxy)methyl @ ethoxy-acetyl oxy propyl-1,4,7,10-tetraazacyclododecane-1,4, -ri- acetato (3-) gadolinium

A) 1,3-Bis (octadecyloxy)-2-propanol (C. A. S. Registry No. 18794- 74-6) Epichlorohydrin (4.6 g; 50 mmol) was added in 5 min to excess stearyl alcohol (117 g; 433 mmol) at 70°C in the presence of 80 NaH mineral oil dispersion (1.65 g; 55 mmol) (commercial product ;. The mixture was stirred for 6 h, then cooled to room temperature and

treated with Et20 (2:. The mixture was filtered and the solution evaporated. The crude product was crystallized four times from acetone to give the desired compound (16.5 g; 27.6 mmol). Yield 55%. K. F. : 0.1%. The 1H-NMR, andIRMS spectra were consistent with the above structure.

Elemental analysis (%): C Calcd. 78.46 13.51 Found 78. 63 13.61 B) 2-(Octadecyloxy)-1-(octadecyloxy) methyl ethOxy-acetic acid (C. A. S. Registry No. 79979-56-9) 80% NaH mineral oii dispersion (2.65 g; 88 mmol) was added under nitrogen atmosphere to a solution of 1,3-bis (octadecyloxy)-2-propanol (6.92 g; 11.6 mmol) in THF (200 mL). The mixture was heated to reflux and a solution of BrCH2COOH (8.1 g; 58 mmol) in THF (50 mL) was added dropwise in 30 min. After another 30 min, MeOH was added, then the solvent was evaporated. The residue was dissolved in Et2O, washed with 0.1 N HC1, dried and evaporated. The crude product was crystallized twice from EtOAc to give the desired compound (5.7 g; 8.7 mmol). Yield 75%. K. F.: 0.47 %; the H-NMR, C-NMR, MS and IR spectra were consistent with the foregoing structure.

Elemental analysis (%): CH Calcd. 75.17 12.62 Found 75.13 13. C1 anhydrous C) The title compound was then prepared, starting from 2- (octadecyloxy)-1-(octadecyloxy) methyl ethoxy acetic acid and the intermediate (A) of Example 6, according to the synthetic method reported in Example 6.

6, 9-Bis (carboxymethvl)-3-f2- (octadecvlamino)-2-oxcet-hyl-11- oxo-3,6,9,12-tetraazatriacontanoato (3-) gadolinium

A) 6,9-Bis (carboxymethyl)-3- 2- (octadecylamino)-2-oxoethyl-11- oxo-3,6,9,12-tetraazatriacontanoic acid (C. A. S. Registry Number 135546-68-8) The product was synthesized following the procedure F. Jasanada and F. Nepveu in Tetrahedron Lett. 33 (1992), 5745-5748. KF: 0,54%.

The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the foregoing structure.

Elemental analysis (%): NCH Calcd. 7.8110.91 Found 7.7811.31 anhydrous B) [6,9-Bis (carboxymethyl)-3- 2- (octadecylamino)-2-oxoethyl-11- oxo-3,6,9,12-tetraazatriacontanoato (3-) gadolinium This product was synthetized according to G. W. Kabalka et al.

Magn. Reson. Med. 19 (1991), 406-415. Yield 82%. Weight loss (130°C): 5.95%. The MS and IR spectra were consistent with the desired structure.

Elemental analysis (%) : C H N Gd Calcd. 57.16 9. 02 6. 67 14.97 Found 57.09 9. 37 6. 57 14.85 6,9-Bis(carboxymethyl)-3-(2-oxo-6,9,12,15,18,21,24-hep-taoxa -3- azapentacosyl)-11-oxo-15,18,21,24,27,30,33-heptaoxa-3,6,9,12 -tetraaza tetratriaconranoato (3-)] gadolinium

A) 6,9-Bis (carboxymethyl)-3-(2-oxo-6,9,12,15,18,21,24-heptaoxa-3- azapentacosyl)-ll-oxo-15, 18, 21, 24, 27, 30, 33-heptaoxa-3, 6, 9, 12-tetraaza tetratriacontanoic acid N,-Bis 2-(2, 6-dioxo-4-morpholinyl) ethyl glycine (commercial product) (8.93 g; 25 mmol) was added to a solution of 17,20-heptaoxadocosan-22-amine (prepared according to WO 95/17380) (16.97 g; 50 mmol) in DMF (250 mL) at room temperature.

After 2 h, the reaction mixture was evaporated and the residue was dissolved in H20 and 6N HC1. The solution (pH 2) was loaded onto an Amberlite XAD-1600 resin column and eluted with a CH3CN/H2O gradient.

The product starts eluting with 10% CH3CN.

The fractions containing the product were evaporated and dried under reduced pressure to give the desired product (14 g; 13.5 mmol).

Yield 54%. HPLC: 96% (area %). K. F.: 1.22%. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the above structure.

Elemental analysis (%) CHN Calcd. 51.00 8. 27 6.76 Found 51.55 8. 35 6.81 anhydrous B) 6,9-Bis (carboxymethyl)-3- (2-oxo-6,9,12,15,18,21,24-heptaoxa-3- azapentacosyl)-11-oXo-15, 18,21,24,27,30,33-hepta-oxa-3,6,9,12-tetraaza tetratriacontanoato(3-)]gadolinium Gd203 (1.86 g; 5.1 mmol) was added to a solution of the free ligand from the previous preparation (10. 62 g; 10.2 mmol) in H20 (200 mL) and the resulting suspension was heated at 50 °C for 7 h. The

reaction mixture was filtered through a Millipore# apparatus (HA 0.45 µm filter); the filtrate was evaporated and dried under reduced pressure to give the title compound (11.9 g; 10 mmol). Yield 98%. Free ligand (0.001 M GdCl3): 0.05% (w/w). HPLC: 98% (area g). K. F.: 1. 59 %.

Weight loss (120°C) : 1.58%. The MS and IR spectra were consistent with the structure.

Elemental analysis (%) : I C H N Gd Calcd. 44.40 6. 94 5. 88 13.21 7.135.9113.23Found44.45 anhydrous EXAMPLE 10 [6,9-Bis(carboxymethyl)-3- (2,16-dioxo-6,9,12-trioxa-3,15-diaza- ririacontanyl)-11,25-dioxo-15,18,21-trioxa-3,6,9,12, 24-pentaazado- etraontanoato (3-) gadolinium A) (C.A.S.

Registry Number 14464-32-5) This compound was synthetized according to M. Shi : itzky and R.

Haimovitz in J. Am. Chem. Soc. 115 (1993), 12545-12549, and Y.

Lapidot, S. Rappoport and Y. Wolman in J. Lipid Res. 8 (1967), 142- 145. Yield: 86%. K. F.: <0.1 %. The 1H-NMR, 13C-NMR and IR spectra were consistent with the structure.

Elemental analysis (%): C H N Calcd. 69.25 10. 30 3.67 Found 69.46 10. 77 3.85 B) (C. A. S. Registry No.

929-75-9) This compound was prepared according to the method disclosed in Liebigs Ann. Chem. 2 (1990), 129-143.

Elemental analysis (%): C N Calcd. 49.98 14.57 Found 49. 68 14.23 C) 13-Oxo-3,6,9-trioxa-12-azatriaconlanylamine H35C17-CONH- (CH2) 2-0 3- (CH2) 2-NH2 1-[(l-Oxooctadecyl) oxy]-2t5-pyrrolidinedione[(l-Oxooctadecyl) oxy]-2t5-pyrrolidinedione (3.87 g; 10.1 mmol) in CHC13 (600 mL) was added dropwise in 6 h to a solution of 3,6,9- trioxaundecane-1,11-diamine (19.8 g; 103 mmol) in CHC13 (100 mL) at 20°C. The reaction mixture was evaporated, the residue treated with CH2Cl2 (50 mL) and the suspended solid filtered off. The solution was evaporated, the residue treated with H2O and extracted with EtOAc. The organic phases were combined and dried. Concentration to small volume led to the precipitation of the desired compound (4.04 g; 8.8 mmol) which was collected by filtration. Yield 87%. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the proposed structure.

Elemental analysis (%): C H N Calcd. 68.08 11. 87 6. 11 Found 67.94 11. 91 5.92 anhydrous D) 6,9-Bis (carboxymethyl)-3- (2,16-dioxo-6,9,12-trioxa-3,15- diazatritriacontanyl)-11,25-dioxo-15,18,21-trioxa-3,6,9,12,2 4- pentaazadotetracontanoic acid N,N-Bis 2- (2, 6-dioxo-4-morpholinyl) ethyl glycine (commercial product) (6.45 g; 18 mmol) was added to a solution of 12-aza-13-oxo- 3,6,9-trioxatriacontanylamine (16.57 g; 36 mmol) in DMF (300 mL) at 70 °C. After 2 h the mixture was cooled to room temperature to give a precipitate that was filtered and washed with acetone. The crude was crystallized twice from acetone. The solid was filtered, washed with acetone and dried under reduced pressure to give the desired compound (17.35 g; 13.6 mmol). Yield 75%. HPLC : 98 % (area %); K. F. : 0.99 %.

Weigth loss (120 °C) : 0.95 %. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the above structure.

Elemental analysis (%): C H N

Calcd. 62.19 7.69 Found 62. 36 10. 00 7. 55 anhydrous E) [6,9-Bis (carboxvmethvl)-3-(2, 16-dioxo-6, 9, 12-trioxa-3,15- diazatritriacontanvl)-11,25-dioxo-15, 8, 21-trioxa-3,6,9,12,24- pentaazadotetracontanoato (3-) gadolinium (CH3COO) 3Gd-4H20 (4.06 g; 10 mmol) was added to a solution of the product from the previous preparation (12.75 g; 10 mmol) in MeOH (600 mL) at 50°C. After 2 h the clear solution was evaporated and dried under reduced pressure to give the title compound (12.3 g; 8.6 mmol).

Yield 86%. Free ligand (0.001 M GdCl3): 0.02 (w/w). HPLC: 100 % (area %); K. F.: 1.90 %. Weight loss (120°C) : 3.02 %. The MS and IR spectra were consistent with the structure.

Elemental analysis (%): NGdCH Calcd 55. 47 8. 75 6. 86 11.00 Found 55.49 8. 88 6. 73 10.66 anhydrous EXAMPLE 11 [6,9-Bis(carboxymethyl)-3- (2,14-dioxo-18,21,24,27,30,33,36- heptaoxa-3,15-diazaheptatriacontanyl)-11,23-dioxo-27,30,33,3 6,39,- 9,12,24-pentaazahexatetracontanoato (3-) gadolinium acidA)11-[[(1,1-Dimethylethoxy)carbonyl]amino]undecanoic (C. A. S. Registry No. 10436-25-6) 11-Aminoundecanoic acid (3 g; 14.9 mmol) was suspended in a 10% solution of Et3N in MeOH (200 mL); dicarbonic acid bis (1,1- dimethylethyl) ester (Boc2O) (3.58 g; 16.4 mmol) was added and the mixture was heated to 50°C for 15 min. Rs soon as the aminoacid dissolved, reaction was complete. After evaporation of the solvent under reduced pressure, the triethylammonium salt of the product was treated with a 20% solution of citric acid ir. H2O and the free acid

was extracted with EtOAc. The organic phase was separated, dried over Na2SO4 and then evaporated under reduced pressure to give the desired compound (4.45 g; 14.1 mmol). Yield 95%. HPLC : 97% (area %); K. F.: <0.1%. Weight loss (60 °C): 0.83%. The"C-NMR,MS and IR spectra were consistent with the proposed structure.

Elemental analysis (%): C H N Calcd. 63. 76 10. 37 4. 65 Found 63. 73 10. 38 4. 63 B) 11-Amino-N- (3, 6, 9, 12, 15, 18, 21-heptaoxadocosyl)- undecanamide To a stirred mixture of ll-(l, 1-dimethyl- ethoxy) carbonyl amino] undecanoic acid (8.8 g; 29.2 mmol), 17,20-heptaoxadocosan-22-amine (prepared accor-ding to WO 95/17380) (10.9 g; 32.1 mmol) and diethyl cyanophosphonate (DEPC) (5.2 g; 32.1 mmol; 4.9 mL) in DMF (200 mL), maintained at 0°C, Et3N (3.3 g; 32.1 mmol; 4.5 mL) was added over 1 h. The reaction mixture was stirred at 0°C for 30 min and then at room temperature for 1 h. The solvent was evaporated under reduced pressure, the crude was dissolved in 1.2 N HC1 in MeOH and the resulting solution was stirred overnight.

(In an analogous preparation the above-mentioned crude was purified by washing the solution of the product in EtOAc with 5% aq.

NaHCO3 and identified as 11-[[(1,1-dimethylethoxy)carbonyl]amino]-N- (3,6,9,12,15,18,21-heptaoxadocosyl) undecanamide: HPLC: 91% (area O).

Weight loss (120 °C): K. F.: 0.64% Elemental analysis (%): C H N Calcd. 59. 7810. 034. 50 Found 59.59 10. 48 4.

Anhydrous The 13C-NMR, MS and IR spectra were consistent with the structure).

After evaporation of the solvent under reduced pressure, the residue was dissolved in a saturated solution of NaHC03 and then washed with EtOAc. The aqueous phase was separated and acidified with IN HC1 until precipitation of the product occurred; the latter was filtered to give 11-amino-N- (3,6,9,12,15,18,21-heptaoxa docosyl) undecanamide salified with 1/3 HC1 (11.9 g; 22.3 mmol). Yield

76%. HPLC: 95' (area +). K. F. : 0.30%. The 13C-NMR, MS and IR spectra were consistent with the foregoing structure.

Elemental analysis (%): C H N C1 Calcd. 58. 38 10. 24 5. 24 2.09 Found 58. 05 10. 13 5. 15 2. 12 anhydrous C) 6,9-Bis (carboxymethvl)-3-(2, 14-dioxo-18,21,24,27,30,33,36-heptaoxa- 30,33,36,39,42,45- heptaoxa-3,6,9,12,24-pentaazahexatetracontanoic acid N, N-Bis 2-(2, 6-dioxo-4-morpholinyl) ethyl glycine (3.8 g; 10.6 mmol) (commercial product) was added to a suspension of 11-amino-N- (3,6,9,12,15,18,21-heptaoxadocosyl) undecan-amide (11.1 g; 21.1 mmol) in DMF (200 mL). After 15 min the reaction mixture became clear and the conversion was complete. The solvent was evaporated under reduced pressure, the residue was dissolved in CHCl3 and washed with H20. The organic phase was separated, dried over Na2SO4 and then evaporated under reduced pressure. The solid was dissolved in H2O and loaded onto an AmberliteX XAD-7 HP resin column (700 mL) and eluted with a CH3CN/H2O gradient. The fraction containing the product was evaporated to give the desired compound (11 g; 7.8 mmol). Yield 74%. HPLC : 85% (area %); K. F. : 0.49%. Weight loss (120 °C) : 0.45%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): C H N Calcd. 56.51 9. 13 6.99 Found 56. 36 9.12 7. 02 D) [6,9-Bis (carboxymethyl)-3-(2,14-dioxo-18, 21,24,27,30,33,36- heptaoxa-3,15-diazaheptatriacontanyl)-11,23-dioxo-27,30,33,3 6,39,- 42,45-heptaoxa-3,6,9,12,24-pentaazahexatetracontanoato (3-) qadolinium Gd203 (C. 9 g; 2.5 mmol) was added to a solution of the free ligand from the previous preparation (7 g; 5 mmol) in EtOH (100 mL) and H2O (150 mL), the resulting suspension was heated at 65°C for 1 h.

The reaction mixture was filtered through a Millipore# apparatus (HVLP type; 0.45 µm filter); the filtrate was evaporated under reduced pressure to give the title compound (7. 5 g; 4.74 mmol). Yield 95%.

Free gadolinium (0.001 M Na2EDTA): <0. 01% (w/w). HPLC : 96% (area %); K. F. : 1. 69%. The MS and IR spectra were consistent with the structure.

Elemental analysis (k): NGdCH Calcd. 50.918. 036. 3010.10 Found 51.06 7. 98 6. 52 9. 94 anhydrous EXAMPLE 12 [[6,9-Bis(carboxymethyl)-16-hydroxy-3-(7-hydroxy-2,7-dioxo-6 ,8- dioxa-3-aza-7-phosphatetracosanyl)-11,16-dioxo-15,17-dioxa-3 ,6,9,12- tetraaza-16-phosphatritriacontanoato (5-) gadolinate (2-)' disodium salt A) Phosphoric acid mono (2-aminoethyl) monohexadecyl ester [C. A. S. Registry Number 57303-02-3] A solution of hexadecyl alcohol (26.2 g; 108 mmol) in THF (100 mL) was added dropwise in 30 min to a solution of POC13 (16.56 g; 108 mmol) and Et3N (12.35 g; 122 mmol) in THF (200 mL) at 0°C. After 5 min a solution of ethanolamine (7.2 g;118 mmol) and EtN(43.51g; 430 mmol) in THF (60 mL) was added dropwise in 60 min. at 0°C. The reaction mixture was allowed to reach room temperature in 3 h; then it was heated at 40 °C and HC1 10 % (100 mL) was added. After 2 h the mixture was cooled to room temperature and addition of H20 (200 mL) afforded a precipitate that was filtered, washed with H2O and zried under reduced pressure to give the desired product (33.4 g; 91 mmol).

Yield 85 %. K. F. : 0.53 4. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (4): C H N P Calcd. 3.838.4711.03 Found 59.07 3.-6 P-. 02 anhydrous

B) 6,9-Bis (carboxvmethyl)-16-hydroxv-3- (7-hyaroxv-2, 7-dioxo-6,8- dioxa-3-aza-7-phosphatetracosanvl)-11, 16-dioxo-15, 17-dioxa-3, 6, 9,12- tetraaza-i6-shosshatritriacontanoic acid N, N-Bis [2-(2,6-dioxo-4-morpholinyl)ethyl]glycine (7. 15 g; 20 mmol) (commercial product) was added to a suspension of phosphoric acid mono (2-aminoethyl) monohexadecyl ester (14.62 g; 40 mmol) in DMF (700 mL) at 75 °C to afford a solution after 15 min. After 5 h the reaction mixture was evaporated and the crude was treated with H2O and 2N HC1 to give a solid that was filtered, washed with H2O and acetone.

The solid was purified by flash chromatography (CH2Cl2/MeOH/NH4OH 25% (w/w) = 6/3/1 (v/v/v)) The fractions containing the product were combined and concentrated to 200 mL. Acidification with 2N HC1 down to pH 1 led to the formation of a precipitate that was filtered, washed with H20 and acetone and dried under reduced pressure to give the desired compound (17 g; 15.6 mmol).

Yield 78 Acidic titer (0.1 N NaOH): 95 %. HPLC: 99 % (area %).

Weight loss (120°C) : 3.45 %; K. F. : 3.06 %. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) : C H N P Calcd. 55.18 9. 17 6. 43 5.69 Found 6.255.448.97 anhydrous C) 6,9-Bis (carboxymethvl)-16-hvdroxv-3- (7-hydroxy-2, 7-dioxo- 17-dioxa- 3,6,9,12-tetraaza-16-phosphatritriacontanoato (5-)] gadolinate (2-) disodium salt (CH3COO)3Gd#4H2O (4. 06 g; 10 mmol) was added to a solution of the free ligand from the previous preparation (10.88 g; 10 mmol) in MeOH (500 mL) and 1 N NaOH (20 mL; 20 mmol). After 24 h the clear solution was evaporated, the residue dissolved in H20 (200 mL) and the solution nanofiltered for 16 h: The retentate was evaporated and dried under reduced pressure to give the title compound (11 g; 8.6 mmol). Yield 86 %. HPLC : 99 % (area Weight loss (120°C) : 4.33 + K. F.: 4.23 c The MS and IR spectra were consistent with the structure.

Elemental analysis (%):

NGdNaPCH Calcd. 46.68 12.223.574.825.44 Found 5.4411.993.484.477.66 anhydrous EXAMPLE 13 6,9-Bis (carboxymethyl)-11,19-dioxo-3-2-2-2-(1-oXoocta- decyl)oxy]ethoxy]ethyl]amino]-2-oxoethyl]-15,18-dioxa-3, 6,9,12-tetra- azahexatriacontanoato (3-) gadolinium A) Octadecanoic acid 2-(2-aminoethoxy) ethyl ester hydrochloride 1.2 M HC1 in CH30H (30 mL) was added to a solution of 2- (2- aminoethoxy) ethanol (2.1 g; 20 mmol) (commercial product) in CH30H (30 mL); after 30 min the solution was evaporated and dried under reduced pressure to give 2- (2-aminoethoxy) ethanol hydrochloride (2.9 g; 20 mmol).

Stearoyl chloride (6.4 g; 21 mmol) (commercial product) was added dropwise in 5 min to a solution of 2- (2-aminoethoxy) ethanol hydrochloride (2.9 g; 20 mmol) in DMF (50 mL) at room temperature to afford a suspension. After 16 h, the suspension was diluted with acetone and the precipitated solid filtered and washed with acetone.

The crude was crystallized from EtOAc; the solid was filtered, washed with EtOAc and dried under reduced pressure =o give the desired compound (3.3 g; 8 mmol). Yield 409. Acidic liter (0.1 N NaOH): 93 %.

K. F. : 1.06---. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental :(%) NClCH Calcd. 3.438.6911.36 Found 3.528.9011.41 anhydrous

B) 6,9-Bis (carboxymethyl)-11,19-dioxo-3-[2-[[2-[2-[(1-oxo- octadecyl) oxy]ethoxy]ethyl]amino]-2-oxoethyl]-15,18-dioxa-3, 6,9,12- tetraaza-hexatriacontanoic acid g;5.5N,N-Bis[2-(2,6-dioxo-4-morpholinyl)ethyl]glycine(1.9 mmol) (commercial product) was added to a solution of octadecanoic acid 2- (2-aminoethoxy) ethyl ester hydrochloride (4. 5 g; 11 mmol) and Et3N (1.5 g; 14 mmol) in DMF (150 mL) at 60°C. After 5 min the temperature was decreased to 45°C. After 2.5 h the mixture was cooled to room temperature to give a precipitate that was filtered, washed with acetone/H2O 15/5, then with acetone. The crude was dissolved in acetone and 0.1 N HCl and the solution heated at 50°C. After 10 min the solution was cooled to room temperature to afford a precipitate which was filtered, washed with acetone/H20 15/5 then with acetone and dried under reduced pressure to give the desired compound (1. 7 g; 1.5 mmol). Yield 27 0. HPLC. 100 % (area %). HPCE : 100 % (area). K. F.: 3.11 %. Weight loss (120 °C) : 4.49 %. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) : C H N Calcd. 63.30 9. 98 6. Found 63.29 9. 95 6.

C) [6,9-Bis (carboxvmethvl)-11,19-dioxo-3-2-2-2-(1- oxooctadecvl) oxy ethoxv ; ethyl ; amino'-2-oxoethyl-15,18-dioxa-3,6,9,12- tetraazahexatriacontanoato (3-)] gadolinium (CH3COO) 3Gd-4H2O (1.11 g; 2.7 mmol) was added to a solution of the free ligand from the previous preparation (3 g; 2.7 mmol) in MeOH (150 mL) at 40°C. After 1 h the clear solution was evaporated and dried under reduced pressure. The crude was purified by flash chromatography (CH30H) to give the title compound (3.2 g; 2.5 mmol).

Yield 94 s. HPCE: 100 % (area %). K. F.: 4.07%. Weight loss (120°C) : 4.08%. the MS and IR spectra were consistent with the structure.

Elemental analysis (%): NGdCH Calcd. 55.528. 525. 5812.53 Found 55.50 8. 52 5. 58 2.62 anhydrous EXAMPLE 14 [[N,N'-[[[2-(Octadecylamino)-2-oxoethyl]imino]di-2,1-ethaned iyl] saltbis[N-(carboxymethyl)glycinato(4-)]]gadolin-are(1-)]sodi um

A) N, N-bis [2-[bis[2-(1,1-dimethylethoxy)-2-oxoethyl]amino]- ethyl glycine The compound was prepared according to the method disclosed in patent application WO-A-95/32741.

B) N, N'- [ [ [2-(Octadecylamino)-2-oxoethyl]imino]di-2,1-ethane- diyl) bis N- 2- (1, 1-dimethvlethoxy)-2-oxoethyl) glycine 1, 1-dimethvl- ethyl ester] Isobutyl chloroformate (205 mg; 1.65 mmol; 215 iL) was added dropwise to a solution of the product from the previous preparation (950 mg; 1.54 mmol) and Et3N (165 mg; 1.65 mmol; 230 µL) in THF (50 mL) at-5°C and under nitrogen. After 15 min a suspension of octadecylamine (450 mg; 1.65 mmol) in THF (50 mL) was added tc the reaction mixture at-5°C. After 20 min the reaction mixture was allowed t rise to room temperature and stirred overnight. The suspension was filtered to remove the residual octadecylamine and the solution was evaporated under reduced pressure. The residue was dissolved in Et20 and the solution washed with 5 aq. NaHC03. The organic phase was dried over Na2SO4 and then evaporated under reduced pressure. The crude product was purified by flash chromatocraphy (CH2Cl2/MeOH = 100/2 (v/v)) to give the desired compound d (1. 1 g; 1.26 mmol). Yield 80%. HPLC : 94% (area. 0. The 13C-NMR, MS and IR spectra were consistent with the structure.

C) N, N'- [ [ [2-(Octadecylamino)-2-oxoethyl]imino]di-2,1-ethane- diyl]bis[N-(carboxymethyl)glycine] 0.5 M H2SO4 (100 mL; 50 mmol) was added dropwise to a solution of the tetraester from the previous preparation (21 g; 24. 3 mmol) in dnoxane (_0 mL) and the resulting mixture was heated at 90°C for 4 h.

The pH o thesolutionwasadjustedtc5with2NNaOH'10mL;anine evaporatedunderreducedpressure.Theresiduewassolventwas

dissolved in CHCl3/MeOH (4: 1) and the resulting suspension was filtered and evaporated under reduced pressure. The crude product was purified by flash chromatography (CH2Cl2/MeOH/NH40H 25% (w/w) = 6/3/1 (v/v/v)). The product was dissolved in H2O (150 mL) and 12 N HCl (7 mL) and desalted by elution through an AmberliteO XAD 7-nP resin column with a CH3CN/H2O gradient. The fractions containing the product were evaporated to give the desired compound (4 g; 6.2 mmol). Yield 25%. HPLC 99% (area %). K. F.: 3.77%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) NCH Calcd. 8.699.38 Found 8.519.21 anhydrous D) [ N, N'- 2- (Octadecvlamino)-2-oxoethylliminoldi-2,1- ethanediyl bis (N- (carboxymethyl) glycinato (4-)]]gadoli-nate(1-)] sodium salt 1 N NaOH (4.5 mL) was added to a suspension of the free ligand from the previous preparation (2.9 g; 4.5 mmol) in 1: 1 H20/CH3CN (600 mL). A solution of GdCl3#6H2O (1.66 g; 4.48 mmol) in H2O (50 mL) was added dropwise to the reaction mixture mantained at pH 6.8 by the addition of 1 N NaOH (13.5 mL). After 2 h the solution was evaporated; the residue was dissolved in H2O and desalted by elution through an AmberliteX XAD 7-HP resin column (250 mL) with a H20/CH3CN gradient.

The fractions containing the product were evaporated. The solid residue was dissolved in H2O and the solution eluted through a Dowex CCR 3LB weak cation exchange resin column (Na form, 20 mL). The eluate was evaporated and dried under reduced pressure to give the title compound (1.35 g; 1.64 mmol). Yield 35%. HPLC : 100% (area %).

K. F.: 8.85%. Weight loss (120 °C): 8. 15%. The MS and IR spectra were consistent with the structure.

Elemental analysis (%) NGdNaCH 6.876.8219.152.80Calcd.46.81 Found 46.79 18.962.686.74 EXAMPLE 15 AT.-Bis2-bis(carboxvmethyl)amino)ethyl!-/V-(l-oxooctadecyi)- L-lysinato (5-) gadolinate (2-) disodium salt

A) N tN2-Bis 2- bis 2- (l, l-dimethylethoxy)-2-oxoethyl-amino- ethyl-L-lvsine 1,1-dimethylethvl ester This product was prepared according to Example 2 of WO 98/05626.

The 13C-NMR, 1H-NMR, MS and IR spectra were consistent with the disclosed structure.

Elemental analysis (%): C H N Calcd. 61.26 9. 74 7.52 Found 61.43 10. 25 7. 48 B) N2, N2-Bis 2-bis 2-(i, l-dimethvlethoxy)-2-oxoethyl amin ethyl-N- (1-oxooctadecyl)-L-lysine- (1, 1-dimethylethyl)-ester Stearoyl chloride (5.45 g; 18 mmol) dissolved in CHCl3 (60 mL) was added dropwise in 1 h to a solution of N2, N-bis 2-bis 2-(1, 1- dimethylethoxy)-2-oxoethyl etnyl-L-lysine 1,1-dimethylethyl ester (13.5 g ; 18 mmol) in CHCl3 (300 mL) at 0°C. After 10 min the reaction mixture was allowed to rise to room temperature and TLC analysis showed the complete conversion of the starting materials. The solution was washed with 5% aq. NaHCO3, the organic phase was separated, dried over Na2SO4 and then evaporated under reduced pressure. The crude product was purified by flash chromatography (n- hexane/EtOAc = 6/4 (v/v)) to give the desired product (11.3 g; 11.2 mmol). Yield 62%. HPLC: 95% (area %). the 13C-NMR, MS and IR spectra were consistent with the structure.

C)N2,N2-Bis[2-[bis(carboxymethyl)amino)ethyl]-N6-(1-oxoocta- decyl)-L-lysine N2,N2-Bis[2-[bis[2-(1,1-dimethylethoxy)-2-oxoethyl]-amino]et hyl]- N6- (l-oxooctadecyl)-L-lysine 1, 1-dimethylethyl ester (9 g, 8.9 mmol)

was dissolved in 6 N HC1 (200 mL) and the solution was stirred for 3 days. The reaction mixture was directly loaded onto an AmberiiteC XAD 7-HP resin column and desalted by elution with a CH3CN/H2O gradient.

The solid obtained from the column was completely converted into the expected acid by treatment with neat CF3COOH for 2 h. CF3COOH was eliminated by repeated dilution with CH2Cl2 and Et20 followed each time by evaporation under reduced pressure. The residue was dried to give the desired product (3.1 g; 4.06 mmol). Yield 45%. HPLC: 94.4% (area %). Weight loss (120°C): 4.22%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): C H N Calcd. 59.16 9. 10 7.67 Found 59.02 9. 38 7.63 D) N2, N2-Bis 2-bis (carboxymethYl) amino ethYl-N-(l-oxoocta- decyl)-L-lysinato (5-)] qadolinate (2-)) disodium salt The free ligand from the previous preparation (2.16 g; 2.96 mmol) was suspended in H2O (50 mL) and dissolved by addition of 1 N NaOH (5.9 mL). Dropwise addition of a 1 M aq. solution of (CH3COO) 3Gd (2.96 mL) led to the precipitation of a solid, which was dissolved by addition of EtOH (150 mL). The solvent was evaporated under reduced pressure to give the title compound (2.57 g; 2.66 mmol). Yield 90%.

HPLC: 99% (area %). K. F. : 3.81%. Weight loss (120 OC) : 3. 84%. The MS and IR spectra were consistent with the structure.

Elemental analysis (%): NGdNaCH Calcd. 46.54 6.62 6.03 16.92 4.95 Found 5.6716.935.426.38 EXAMPLE16 <BR> <BR> [[N2,N2-Bis[2-[bis(carboxymethyl)amino]ethyl]-N6-[@[(3ß)-ch olest-5-<BR> <BR> salten-3-yl]oxy]carbonyl]-L-lysinato(5-)]gadolinate(2-)]diso @ium

A) N2'N2-Bis 2-bis 2-(1, 1-dimethylethoxy)-2-oxoethyl-amino- ethyl-N6- (3)-cholest-5-en-3-yljoxy carbonyl-L-lysine 1, 1-di- methylethyl ester A solution of cholesteryl chloroformate (11 c; 22 mmol) (commercial product) in CHC13 (60 mL) was added in 1 h to a solution of N2, N2-bis [2-[bis[2-(1,1-dimethylethoxy)-2-oxoethyl]amino]ethyl]-L- lysine 1,1-dimethyl ethyl ester (14.9 g; 20 mmol) (prepared according to Example 2 of WO 98/05626) in CHC13 (150 mL). The reaction mixture was stirred overnight. The solution was then washed with 5% aq.

NaHCO3, the organic phase was separated, dried over Na2SO4 and then evaporated under reduced pressure The crude product was purified by flash chromatography (n-Hexane/EtOAc = 85/15 (v/v)) to give the desired compound (18.2 g; 15.7 mmol). Yield 78%. HPLC : 94% (area %).

Weight loss (100 °C) : 1.23%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): C H N Calcd. 68.48 10. 10 4.84 Found 68. 89_ 10. 08 4.75 B) N2, N2-Bis 2- bis (carboxvmethvl) amino ethyl-N6- (3)- cholest-5-en-3-yl oxy carbonyl-L-lvsine 12.6 g of the product from the previous preparation (10.88 mmol) were dissolved in formic acid (250 mL) and refluxed for 1.5 h. The solution was evaporated under reduced pressure, the crude was suspended in water, stirred for 30 min and filtered to give the desired compound (7.8 g; 8.5 mmol). Yield 79%. HPLC: >91% (area %).

K. F. : 3.73%. The 13C-NMR, MS and IR spectra were consistent with this structure.

Elemental analysis (%): CHN Calcd. 62.99 8. 73 6.39 Found 62.70 8. 89 6.31 anhydrous C) N2, N2-Bis 2- bis (carboxvmethvl) amino ethyl-N6- (3a)- cholest-5-en-3-yl oxv carbonvl-L-lysinato (5-) gadolinate (2-)] disodium salt 5.3 g of the free ligand from the previous preparation (5.8 mmol) were suspended in H20 (300 mL) and dissolved by the addition of 2 N NaOH (5.8 mL). A solution of GdCl3-6H20 (2.16 g; 5.8 mmol) in H20 (10 mL) was added dropwise to the reaction mixture mantained at pH 6.8 by the addition of 2 N NaOH (8.7 mL). After 1 h the solution was concentrated to 50 mL, the addition of CH3CN led to the precipitation of the title compound, which was filtered and dried (4.94 g; 4.60 mmol). Yield 79%. HPLC: >89% (area %). K. F.: 10.12%. Weight loss (120 °C): 11.89%. The MS and IR spectra were consistent with the structure.

Elemental analysis (%): C H N Gd Na 6.66Calcd.51.38 5.21 4.28 6.835.1714.514.02Found51.47 EXAMPLE 17 10- 2- 2- 2- (Dioctadecylamino)-2-oxoethoxy-2-oxoethyl amino- 2-oxoethyl-1,4,7,10-tetraazacyclododecane-1,4,7-triacetato (3- )gadolinium A) N-Octadecyl-1-octadecanamine (diociadecylamine) ; C. A. S.

Registry No. 112-99-2.

A1) Dioctadecvlcvanamide (C. A. S. Registry No. 113576-09-3) Cyanamide (5 g; 119 mmol) was added to stirred 50% aq. NaOH (100 g). The mixture was cooled to 25°C, hen a solution of Aliquat-336

(trioctylmethylammonium chloride) (2.42 g; 6 mmol (commercial product) and 1-bromooctadecane (40.37 g; 121 mmol) in toluene (50 mL) was added. The mixture was vigorously stirred at 55°C for 6 h. The organic phase was separated and evaporated to give the desired compound (38 g). The crude product was used in the hydrolysis step without any further purification. K. F.: < 0.1%. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) : C H N Calcd. 81. 24 13.64 5. 12 Found 80.86 13. 91 5.19 A2) N-Octadecyl-1-octadecanamine (dioctadecylamine) The crude dioctadecylcyanamide (38 g) was suspended in 2.75 M H2SO4 (150 mL) and the mixture was refluxed for 2.5 h. After cooling to room temperature H20 (100 mL), 30% NaOH (100 mL) and CHC13 (300 mL) were added. The organic phase was separated, dried and evaporated. The solid residue was suspended in Et20 and stirred for 1 h. The solid was filtered and washed with to give the desired compound (15.3 g; 29.3 mmol). Yield 48%. K. F. : 0. 20%. The 1H-NMR, 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) : I C I H I N Calcd. 82.83 14. 48 2.68 Found 82.61 14. 65 2. anhydrous B) 2-Hydroxy-N, N-dioctadecvlacetamide (Acetyloxy) acetyl chloride (3.8 g; 28.1 mmol) (commercial product) dissolved in CHCl3 (150 mL) was added dropwise to a solution of dioctadecylamine (13.3 g; 25.5 mmol) and Et3N (3.9 mL; 28.1 mmol) in CHCl3 (350 mL) and the solution was stirred at room temperature overnight. MeOH (250 mL) and 2 N NaOH (50 mL) were added to the solution. H20 was added to the reaction mixture and a two phase system was obtained. The lower organic layer was separated and evaporated.

The solid residue was suspended in n-hexane and filtered to give the desired compound (12.1 g; 20.9 mmol). Yield 829. HPLC: 96% (area 9).

K. F.: <0.1%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis «): NCH Calcd. 78.69 2.41 Found 2.4413.44 C) N- (Phenylmethoxv) carbonyl) qlycine f2- (dioctadecvlamino)-2- oxoethyl ester A solution of DCC (2.1 g; 10.3 mmol) in CHC13 (50 mL) was added dropwise to a solution of 2-hydroxy-N, N-dioctadecylacetamide (5 g; 8.6 mmol) and Z-glycine (2 g; 9.5 mmol) in CHC13 (250 mL). DMAP (0.1 g; 0.9 mmol) was added to the resulting solution. After 1 h the reaction mixture was filtered and the solvent was evaporated. The crude was purified by flash chromatography (n-Hexane/EtOAc = 7/3 (v/v)) to give the desired compound (5.6 g; 7.3 mmol). Yield 84%. HPLC : 99% (area %). K. F.: <0. 1%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) : I c I H N Calcd. 11. 24 3.63 Found 11. 47 3.68 D) Glycine [2-(dioctadecylamino)-2-oxoethyl] ester hydrochloride 10% Pd/C (150 mg) was added to a solution of N-[(phenylmethoxy)- carbonyl] glycine [2-(dioctadecylamino)-2-oxoethyl] ester (1.2 g; 1.4 mmol) in EtOAc (100 mL) and the suspension was stirred for 3 h under hydrogen atmosphere at room temperature. After filtratior. (through a Millipore filter FT 0.45 Hm) 1.2 M HC1 in MeOH (1.3 mL; 1.6 mmol) was added dropwise to the resulting solution obtaining the precipitation of a white solid that was filtered to give the desired compound (830 mg; 1.2 mmol). Yield 86%. HPLC: 100% (area %). K. F. : 0.22%. The MS and IR spectra were consistent with the structure.

Elemental analysis NClCH Calcd. 71.33 12. 48 4. 16 5.26 12.484.305.30Found71.72 anhydrous E) 1, 4, 7,10-tetraazacvciododecane-1 4,7,10-tetraacetic acid tris (phenylmethyl) ester El) 1,4,7,10-Tetraazacvcìododecane-l-acetic acid (1, 1- ester

A solution of t-butyl bromoacetate (25.3 g; 130 mmol) in CHCl3 (500 mL) (commercial product) was added dropwise in 7 h to a solution of 10-tetraazacyclododecane'112.3 g; 650 mmol) (commercial product) in CHC13 (2 L) maintained under nitrogen at room temperature.

After 14 h the solution was concentrated to 800 mL, washed with H2O, dried and evaporated to give the desired compound (39 g; 129 mmol).

Yield 99%. GC: 92 % (area %). K. F.: 0.42 %. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): NNaClCH 10.0618.56==Calcd.56.20 Found 56. 36 10. 34 18. 84 <0.1 4.56 anhydrous E2) 1,4,7,10-Tetraazacvclododecane-1,4, 7-tetraacetic acid (1,1- dimethyl-ethyl) tris (phenylmethyl) ester A solution of 10-tetraazacyclododecane-1-acetic acid (1,1- dimethylethyl) ester (36 g; 126 mmol) in DMF (200 mL) was added dropwise in 7 h to a suspension of benzyl bromoacetate (94.96 g; 414 mmol) and K2CO3 (86.8 g; 628 mmol) in DMF (250 mL) maintained under nitrogen at room temperature. After 14 h the suspension was filtered and the solution evaporated to dryness. The residue was dissolved in EtOAc, washed with H2O, then with brine. The organic phase was separated, dried over Na2SO4 and evaporated. The residue was purified by flash chromatography (CH2Cl2/MeOH = 15/1 (v/v)) to give the desired compound (51 g; 65 mmol). Yield 51 %. HPLC: 90 % (area %). K. F.: 0.48 %. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): NNaClCH Calcd. 62.38 6.91 7.10 2.91 4.49 Found 6.902.904.956.74 anhydrous E3) 7-tetraacetic acid tris (phenyl-methyl) ester 1,4,7,10-Tetraazacyclododecane-i, 4, 7,10-tetraacetic acid (1,1- dimethyl-ethyl) tris (phenylmethyl) ester adduct with NaCl (47.11 g; 60 mmol) was dissolved in dioxane (500 m ;,). The solution was treated with 12 N HC1 (500 mL) under nitrogen at room temperature, obtaining a precipitate. After 16 h the suspension was evaporated and the residue dissolved in H2O by ultrasound sonication. The solution (pH 2) was loaded onto an Amberlite XAD-1600 resin c iumn (900 mi and eluted

with a CH3CN/H2O gradient. The fractions containing the product were concentrated to remove CH3CN, then extracted with EtOAc. The organic phase was dried over Na2SO4 and evaporated. The residue was triturated with EtOAc to give the desired compound (21 g; 31 mmol). Yield 52 %.

HPLC: 99 % (area %). K. F.: < 0.1 %. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): C H N Calcd. 65.87 8.30 Found 66.007. 038.33 F) 10-[[2-[2-[2-(Dioctadecylamino)-2-oxoethoxy]-2-oXoethyl]- amino-2-oXoethyl-1,4,7,10-tetraazacyclododecane-1,4,7-triace tic acid tris (phenylmethyl) ester To a suspension of 1,4,7,10-tetraazacyclododecane-1,4,7,10- tetraacetic acid tris (phenylmethyl) ester (2.7 g; 4 mmol) and glycine [2-(dioctadecylamino)-2-oxoethyl] ester hydrochloride (3 g; 4.4 mmol) in CHCL3 (250 mL) was added DIEA (diisopropylethylamine) (1.5 mL; 8.8 mmol). BOP ( (benzotriazol-1-yloxy) tris (dimethylamino) phosphonium hexafluorophosphate, commercial product) (2.2 g; 4.8 mmol) was added to the resulting solution, which was stirred at room temperature for 2 h. The solvent was evaporated and the solid residue was suspended in 9: 1 i-PrOH/H2O and filtered to obtain the desired compound (4.9 g; 3.8 mmol). Yield 95%. The 13C-NMR, MS and IR spectra were consistent with the given structure.

Elemental analysis (%) : C H N Calcd. 71.48 9. 66 6.50 Found 71. 239. 546.38 G)10-[[2-[2-(2-(Dioctadecylamino)-2-oxoethoxy]-2-oxoethyl]- aminol-2-oXoethvl-1, 4, 7, 10-tetraazacvclododecane-1, 4, 7-triacetic acid 10% Pd/C (150 mg) was added to a solution of 10-2-2-2- (dioctadecylamno)-2-oxoethoxy-2-oxoethyl) amino-2-oxoethyl-1, 4, 7,- 10-tetraazacycododecane-1,4,7-triacetic acid tris (phenylmethyl) ester (1.5 g; 1.2 mmol) in CH3COOH (150 mL) and the suspension was stirred for 8 h under hydrogen atmosphere at room temperature. After filtration (through a Millipore# filter FT 0.45 Hm) the solvent was evaporated and the residue was dried under reduced pressure to give

the desired compound (3 g; 1 mmol). Yield 83%. HPLC : 97% (area %).

The C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): NCH Calcd. 65.72 10. 44 8.21 Found 65.54 10. 22 8.07 H) 10-2-2-2-(Dioctadecvlamino)-2-oxoethoxy-2-oXoethyl- aminol-2-oxoethyl-1, 4,7,10-tetraazacyclododecane-1,4,7-triacetato (3- ) gadolinium The free ligand from the previous preparation (2.2 g; 2.2 mmol) was dissolved in 3: 1 EtOH/H2O (120 mL); a 0.5 M aq. solution of (CH3COO) 3Gd (4.4 mL) was added dropwise. The resulting solution was heated at 50°C for 4 h. The solvent was evaporated to give the title compound (2.2 g; 1.9 mmol). Yield 86%. HPLC: 95% (area %). The MS and IR spectra were consistent with the structure.

Elemental analysis (%) : C H N Gd Calcd. 57.11 8. 82 7. 14 13.35 Found J EXAMPLE 18 10-1-Methylene-14-octadecyl-2,10,13-trioxo-6,9-dioxa-3,14- diazadotriacontanyl-1,4,7,10-tetraazacyclododecane-1,4,7- triacetato (3-) gadolinium A) 4- (Dioctadecylamino)-4-oxobutanoic acid (C. A. S. Registry No.

37519-63-4) A suspension of dioctadecylamine (24.5 g; 47 mmol) (prepared according to Example 17, Step A) and succinic anhydride (4.7 g; 47 mmol) in THF (100 mL) was stirred at room temperature for 18 h.

Solvent was removed by evaporation and the residue was dissolved in CH2Cl2. mhe solution was washed with 1 N HC1, dried and evaporated.

The crude was crystallized from CH3CN to give the desired compound (21.8 g; 35 mmol !. Yield 75%. K. F.: 0.37%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): NCH Calcd. 2.2512.80 13.382.36Found77.52 anhydrous

B) [10-[1-Methylene-14-octadecy1-2,10,13-trioxo-6,9-dioxa-3,14- diazadotriacontanyl-1,4,7,10-tetraazacyclo-dodecane-1,4,7- triacetato (3-)] gadolinium 4- (Dioctadecylamino)-4-oxobutanoic acid (7.6 g; 12.3 mmol) in CHC13 (100 mL) was added to a solution of 10- 2- 2- (2- hydroxyethoxy) ethyl] amino-1- (methylene)-2-oxoethyl-1,4,7,10- tetraazacyclododecane-1,4,7-triacetato (3-)] gadolinium (8.1 g; 12.3 mmol) (prepared according to Example 2 of WO 96/04259) in DMSO (100 mL), then 1- (3-dimethylaminopropyl)-3-ethylcarbodiimmide hydrochloride (EDCI) (2.6 g; 13.4 mmol) and 4-dimethylaminopyridine (DMAP) (0.75 g; 6.1 mmol) were added and the clear solution was stirred at room temperature. After 24 h more EDCI (2.6 g; 13.4 mmol) was added. After another 24 h CH3CN and H20 were added to obtain the precipitation of a solid, which was filtered with a paper filter. The solid was dissolved in 1/1 CH2Cl2/MeOH and the solution loaded onto a silica gel flash column (CH2Cl2/MeOH = 1/1 (v/v) (5 L); CH2Cl2/MeOH/H2O = 5/5/1 (v/v/v) (4 L)). The fractions containing the product were evaporated to give the title compound (6 g; 4.7 mmol). Yield 38%. HPLC : 98% (area %).

K. F.: 3.02 9. The MS and IR spectra were consistent with the structure.

Elemental analysis (%) : NGdCH Calcd.6.6612.468.87 Found 6.6612.388.92 anhydrous EXAMPLE 19 10- 2- (2- (Dioctadecylamino)-2-oxoethoxy-2-oxoethyl-1, 4,7,10- tetraazacyclododecane-1,4, 7-triacetato (3-) gadolinium

A) 10- 2- 2- (Dioctadecylamino)-2-oxoethoxy-2-oxoethyl-1, 4, 7, IQ- tetraazacyclododecane-1,4,7-triacetic acid tris- (phenylmethyl) ester DBU 020 µL ; 5.5 mmol) was added to a suspension of 10- tetraazacyclododecane-1,4,7 10-tetraacetic acid tris (phenylmethyl) ester (prepared according to Step E3 of Example 17) (3.71 g; 5.5 mmol) in toluene (350 mL) obtaining a clear solution, then 2-bromo-N, N- dioctadecylacetamide (prepared according to Example 5, Step A) (3.9 g; 6.05 mmol) dissolved in toluene (50 mL) was added dropwise. After 2 h the reaction mixture was filtered and the solvent was evaporated. The crude was suspended in CH3CN (50 mL) and the insoluble was filtered off with a paper filter. The solution was evaporated obtaining the desired product (4.63 g; 3.74 mmol). Yield 68%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) : C H N Calcd. 72.83 9. 86 5.66 Found 72. 679. 585.71 anhydrous B) 10-2-2-(Dioctadecylamino)-2-oxoethoxy-2-oxOethyl- acid 10% Pd/C (0.4 g) was added to a solution of the product from the previous preparation (4.9 g; 4 mmol) in CH3COOH (400 mL) and the suspension was stirred for 6 h under hydrogen atmosphere (consumed H2: 270 mL; 12 mmol) at room temperature. After filtration through a Millipores filter FT 0.45 µm the solvent was evaporated under reduced pressure and the residue was dried (1.3 kPa; NaOH pellets; 35°C) to give the desired compound (3.1 g; 3.2 mmol). Yield 80%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%): C H N Calcd. 67.11 10. 74 7.25 Found @ anhydrous C) 10- 2- 2- (Dioctadecylamino)-2-oxoethoxy)-2-oxoethyl-1, 4,7,10- tetraazacyclododecane-1,4,7-triacetato (3-) gadolinium The free ligand from the previous preparation (3.6 g; 3.7 mmol) was dissolved in 2: 1 i-PrOH/H2O (250 mL) and a 0.5 M aq. solution of (CH3COO) 3Gd ;,. 4 mL) was added dropwise. The resulting solution was heated at 5C°C for 6 h. The solvent was evaporated to give the title compound (3.6 g; 3.2 mmol). Yield 87%.

MS and IR spectra were consistent with the structure.

Elemental :(%) C H N Gd Calcd. 57.88 8. 99 6. 25 14.03 Found 57. 69 8. 88 6. 31 13.95 anhydrous EXAMPLE 20 [10-[2-[Bis[2-[(1-oxohexadecyl)oxy]ethyl]amino]-2-oxoethyl]- 1,4,7,10- tetraazacyclododecane-1,4,7-triacetato (3-)] gadolinium A) Hexadecanoic acid iminodi-2,1-ethanediyl ester hydrochloride (C. A. S. Registry No. 84454-85-3) Palmitoyl chloride (29.8 g; 108.4 mmol) (commercial product) was added dropwise in 30 min to a solution of diethanolamine hydrochloride (7 g; 49.4 mmol) (commercial product) in DMF (100 mL). After standing for 1.5 h a white solid crystallized. MeOH (350 mL) was added and the reaction mixture heated to reflux. After cooling to room temperature the recrystallized product was filtered to give as a white solid hexadecanoic acid iminodi-2,1-ethanediyl ester hydrochloride (15 g; 24.2 mmol). Yield 49%. K. F.: 0.4%. The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) : NClCH 11.732.265.73Calcd.69.92 11.742.335.82Found69.79 anhydrous B) 10-[2-[Bis[2-[(1-oxohexadecyl)oxy]ethyl]amino]-2-oxoethyl]- 7-triaceticacidtris (phenylmethyl)- ester A 50% solution of 1-propanephosphonic acid cyclic anhydride (14.4 g; 22.6 mmol) in EtOAc (commercial product) was added to a solution of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid tris (phenyl- methyl) ester (15 g; 22.2 mmol) (prepared according to Step E3 of Example 17), hexadecanoic acid, nminodi-2, 1-ethanediyì ester

hydrochloride (14 g; 22.6 mmol) and Et3N (6. 5 mL; 46. 6 mmol) in CH2C12 (200 mL). The reaction mixture was stirred at room temperature for 24 h, then more 1-propanephosphonic acid cyclic anhydride (14.4 g; 22.6 mmol) was added. After another 24 h the mixture was washed with brine, dried and evaporated. The residue was purified by flash chromatography (CH2Cl2/MeOH = 9/1 (v/v)) to give the desired compound (17 g; 13.7 mmol). Yield 62%. HPLC: 96% (area %). The C-NMR, MS and IR spectra were consistent with the proposed structure.

Elemental analvsis (%): NCH Calcd.9.659.36 9.385.48Found70.57 anhydrous C) 10-2-Bis 2-(l-oxohexadecyl) oxy ethvl amino-2-oXoethyl- 7-triacetic acid 10% Pd/C (1.6 g) was added to a solution of 10- 2- bis 2- (1- oxohexadecyl) oxy] ethyl] amino-2-oxoethyl-1,4,7,10-tetraazacyclo-dodecane- 1,4,7-triacetic acid tris (phenylmethyl) ester (16 g; 12.9 mmol) in EtOH (500 mL) and the suspension was stirred for 12 h under hydrogen atmosphere at room temperature. After filtration through a Milliporee filter FT 0.45 Fm the solution was evaporated under reduced pressure to give the desired compound. Yield 93%. HPLC : 98% (area %). The 13C-NMR, MS and IR spectra were consistent with the structure.

Elemental analysis (%) : C H N Calcd. 64.50 10. 10 7.23 Found 64.38 10. 01 7.15 anhydrous D) 10- 2- Bis 2- (1-oxohexadecyl) oxy] ethyl] amino-2-oxoethyl- 1,4,7,10-tetraazacyclododecane-1,4, 7-triacetato-(3)]gadolinium (CH3COO) 3Gd'4H20 (4.63 g; 11.4 mmol) was added to a solution of the free ligand from the previous preparation (11 g; 11.4 mmol) in EtOH (500 mL) at 50°C. After 6 h the solution was evaporated and dried under reduced pressure to give the title compound (12.3 g; 11 mmol).

Yield 96%. HPLC: 99% (area %). The MS and IR spectra were consistent with the structure.

Elemental analysis (%): S d Calcd. 6.2414.018.44 Found 55.57 8.41 6.12 13.92