Title:
ANTI-PD-L1 ANTIGEN BINDING PROTEIN, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/063352
Kind Code:
A1
Abstract:
Provided is an anti-PD-L1 antigen binding protein, capable of binding to primate-derived PD-L1 with a KD value of 1×10-8M or less. The antigen binding protein can block the binding of PD-1 and CD80 to PD-L1, stimulate the secretion of cytokines in immune cells, and can inhibit tumor growth and/or tumor cell proliferation. Also provided is a fusion protein, comprising human TGFBRII or a fragment thereof and the antigen binding protein. Also provided is an application of the antigen binding protein and/or the fusion protein in the prevention and treatment of tumors or cancers.
Inventors:
HE YUN (CN)
REN SHUWEN (CN)
LIU BIN (CN)
GAN XIN (CN)
LI DONGHAI (CN)
CHEN FEI (CN)
WANG LING (CN)
LI JIN (CN)
RONG YIPING (CN)
REN SHUWEN (CN)
LIU BIN (CN)
GAN XIN (CN)
LI DONGHAI (CN)
CHEN FEI (CN)
WANG LING (CN)
LI JIN (CN)
RONG YIPING (CN)
Application Number:
PCT/CN2020/118811
Publication Date:
April 08, 2021
Filing Date:
September 29, 2020
Export Citation:
Assignee:
HARBOUR BIOMED SHANGHAI CO LTD (CN)
International Classes:
A61K38/17; C07K16/28; A61K39/395; A61P35/00; C07K19/00; C12N15/13; C12P21/06
Domestic Patent References:
| WO2016162368A1 | 2016-10-13 | |||
| WO2018205985A1 | 2018-11-15 |
Foreign References:
| CN106939047A | 2017-07-11 | |||
| CN106939047A | 2017-07-11 | |||
| CN104961829A | 2015-10-07 | |||
| CN104961829A | 2015-10-07 | |||
| CN106103488A | 2016-11-09 | |||
| US20190169621A1 | 2019-06-06 | |||
| US20190218296A1 | 2019-07-18 |
Other References:
VALECHA G. K.: "Anti–PD-1/PD-L1 antibodies in non-small cell lung cancer: the era of immunotherapy", EXPERT REVIEW OF ANTICANCER THERAPY, vol. 17, no. 1, 23 November 2016 (2016-11-23), pages 47 - 59, XP055798118
LUAN Y. ET AL.: "A fully human monoclonal antibody targeting PD-L1 with potent anti-tumor activity", INTERNATIONAL IMMUNOPHARMACOLOGY, vol. 31, 12 January 2016 (2016-01-12), XP029412996, DOI: 10.1016/j.intimp.2015.12.039
KABAT ET AL.: "Protein Sequences of Immunology", 1991, NATIONAL INSTITUTES OF HEALTH
AL-LAZIKANI ET AL., J MOL BIOL, vol. 273, 1997, pages 927 - 48
ISHIDA ET AL., EMBO J, vol. 11, 1992, pages 3887 - 3395
OKAZAKI ET AL.: "Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice", SCIENCE, vol. 291, 2001, pages 319 - 22
FREEMAN ET AL.: "Engagement of PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation", J. EXP.MED., vol. 192, 2000, pages 1027 - 1034, XP002942545, DOI: 10.1084/jem.192.7.1027
CARTER ET AL.: "PD-1: PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2", EUR. J. IMMUNOL., vol. 32, 2002, pages 634 - 643, XP002980901, DOI: 10.1002/1521-4141(200203)32:3<634::AID-IMMU634>3.0.CO;2-9
"NCBI", Database accession no. NP -001271065
"Uniprot", Database accession no. P37173
"Epitope Mapping Protocols in Methods in Molecular Biology", vol. 66, 1996
CUNNINGHAMWELLS, SCIENCE, vol. 244, 1985, pages 1081
W. R. PEARSOND. J. LIPMAN: "Improved tools for biological sequence comparison", PROC. NATL. ACAD. SCI., vol. 85, 1988, pages 2444 - 2448
D. J. LIPMANW. R. PEARSON: "Rapid and sensitive protein similarity searches", SCIENCE, vol. 227, 1989, pages 1435 - 1441
S. ALTSCHULW. GISHW. MILLERE. W. MYERSD. LIPMAN: "A basic local alignment search tool", J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
SAMBROOK J.FRITSCH, E. F.MANIAIS, T.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
AUSUBE ET AL.: "Current Protocols in Molecular Biology", 1993, GREENE PUBLISHING AND WILEY-INTERSCIENCE
LUAN Y. ET AL.: "A fully human monoclonal antibody targeting PD-L1 with potent anti-tumor activity", INTERNATIONAL IMMUNOPHARMACOLOGY, vol. 31, 12 January 2016 (2016-01-12), XP029412996, DOI: 10.1016/j.intimp.2015.12.039
KABAT ET AL.: "Protein Sequences of Immunology", 1991, NATIONAL INSTITUTES OF HEALTH
AL-LAZIKANI ET AL., J MOL BIOL, vol. 273, 1997, pages 927 - 48
ISHIDA ET AL., EMBO J, vol. 11, 1992, pages 3887 - 3395
OKAZAKI ET AL.: "Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice", SCIENCE, vol. 291, 2001, pages 319 - 22
FREEMAN ET AL.: "Engagement of PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation", J. EXP.MED., vol. 192, 2000, pages 1027 - 1034, XP002942545, DOI: 10.1084/jem.192.7.1027
CARTER ET AL.: "PD-1: PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2", EUR. J. IMMUNOL., vol. 32, 2002, pages 634 - 643, XP002980901, DOI: 10.1002/1521-4141(200203)32:3<634::AID-IMMU634>3.0.CO;2-9
"NCBI", Database accession no. NP -001271065
"Uniprot", Database accession no. P37173
"Epitope Mapping Protocols in Methods in Molecular Biology", vol. 66, 1996
CUNNINGHAMWELLS, SCIENCE, vol. 244, 1985, pages 1081
W. R. PEARSOND. J. LIPMAN: "Improved tools for biological sequence comparison", PROC. NATL. ACAD. SCI., vol. 85, 1988, pages 2444 - 2448
D. J. LIPMANW. R. PEARSON: "Rapid and sensitive protein similarity searches", SCIENCE, vol. 227, 1989, pages 1435 - 1441
S. ALTSCHULW. GISHW. MILLERE. W. MYERSD. LIPMAN: "A basic local alignment search tool", J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
SAMBROOK J.FRITSCH, E. F.MANIAIS, T.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
AUSUBE ET AL.: "Current Protocols in Molecular Biology", 1993, GREENE PUBLISHING AND WILEY-INTERSCIENCE
Attorney, Agent or Firm:
SHANGHAI DIANSHI PARTNERS, P.C. (CN)
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