Title:
ANTIBODY AGAINST SPIKE PROTEIN OF SARS-CoV-2
Document Type and Number:
WIPO Patent Application WO/2022/158497
Kind Code:
A1
Abstract:
In this invention, a hybridoma is prepared using, as the antigen, a protein with its secretion signal added to the receptor-binding domain (RBD, positions 319-589) of SARS-CoV-2 spike protein and G196 tag sequence (DLVPR) with His6 sequence (HHHHHH) fused to the C terminus, to obtain a plurality of monoclonal antibodies that recognizes the three-dimensional structure of the RBD. The obtained monoclonal antibodies have a high neutralization activity against the virus, and can be used for a pharmaceutical composition, a test reagent, and a virus removing material. Furthermore, since antibodies having different binding ability against various variants are obtained, the antibodies can also be used for identifying the variants.
Inventors:
URANO TAKESHI (JP)
NARIAI YUKO (JP)
MORITA KOUICHI (JP)
NARIAI YUKO (JP)
MORITA KOUICHI (JP)
Application Number:
PCT/JP2022/001859
Publication Date:
July 28, 2022
Filing Date:
January 19, 2022
Export Citation:
Assignee:
MABPROTEIN CO LTD (JP)
International Classes:
C12N15/13; A61K39/395; A61P31/14; C07K16/10; C07K17/00; C12N7/02; C12P21/08; G01N33/531; G01N33/569
Foreign References:
| US10787501B1 | 2020-09-29 | |||
| KR102205028B1 | 2021-01-20 |
Other References:
HUANG YANG, SUN HUI, YU HAI, LI SHAOWEI, ZHENG QINGBING, XIA NINGSHAO: "Neutralizing antibodies against SARS-CoV-2: current understanding, challenge and perspective", ANTIBODY THERAPEUTICS, vol. 3, no. 4, 8 December 2020 (2020-12-08), pages 285 - 299, XP055953140, DOI: 10.1093/abt/tbaa028
LIU LIHONG; WANG PENGFEI; NAIR MANOJ S.; YU JIAN; RAPP MICAH; WANG QIAN; LUO YANG; CHAN JASPER F.-W.; SAHI VINCENT; FIGUEROA AMIR;: "Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 584, no. 7821, 22 July 2020 (2020-07-22), London, pages 450 - 456, XP037223585, ISSN: 0028-0836, DOI: 10.1038/s41586-020-2571-7
LIU LIHONG; WANG PENGFEI; NAIR MANOJ S.; YU JIAN; RAPP MICAH; WANG QIAN; LUO YANG; CHAN JASPER F.-W.; SAHI VINCENT; FIGUEROA AMIR;: "Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 584, no. 7821, 22 July 2020 (2020-07-22), London, pages 450 - 456, XP037223585, ISSN: 0028-0836, DOI: 10.1038/s41586-020-2571-7
Attorney, Agent or Firm:
SHIRAKATA Yumiko (JP)
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