BACKGROUND OF THE INVENTION The ecteinascidins are exceedingly potent antitumour agents isolated from the marine tunicate Ecteinascidia turbinata. Several ecteinascidins have been reported previously in the patent and scientific literature.

U. S. Patent N° 5, 256, 663 describes pharmaceutical compositions comprising matter extracted from the tropical marine invertebrate, Ecteinascidia turbinata, and designated therein as ecteinascidins, and the use of such compositions as antibacterial, anti-viral, and/or antitumour agents in mammals.

U. S. Patent N° 5, 089, 273 describes novel compositions of matter extracted from the tropical marine invertebrate, Ecteinascidia turbinata, and designated therein as ecteinascidins 729, 743, 745, 759A, 759B and 770. These compounds are useful as antibacterial and/or antitumour agents in mammals.

U. S. Patent N° 5, 478, 932 describes ecteinascidins isolated from the Caribbean tunicate Ecteinascidia turbinata, which provide in vivo protection against P388 lymphom, B16 melanoma, M5076 ovarian sarcoma, Lewis lung carcinoma, and the LX- 1 human lung and MX-1 human mammary carcinoma xenografts.

U. S. Patent N° 5, 654, 426 describes several ecteinascidins isolated from the Caribbean tunicate Ecteinascidia turbinata, which provide in vivo protection against P388 lymphoma, B16 melanoma, M5076 ovarian sarcoma, Lewis lung carcinoma, and the LX-1 human lung and MX-1 human mammary carcinoma xenografts.

U. S. Patent N°. 5, 721, 362 describes a synthetic process for the formation of ecteinascidin compounds and related structures.

WO 00/69862, from which the present application claims priority, describes the synthesis of ecteinascidin compounds from cyanosafracin B.

The interested reader is also referred to : Corey, E. J., J. Am. Chem. Soc., 1996, 118 pp. 9202-9203 ; Rinehart, et al., Journal of National Products, 1990,"Bioactive Compounds from Aquatic and Terrestrial Sources", vol. 53, pp. 771-792 ; Rinehart et al., Pure and Appl. Chem., 1990,"Biologically active natural products", vol 62, pp. 1277- 1280 ; Rinehart, et al., J. Org. Chem., 1990,"Ecteinascidins 729, 743, 745, 759A, 759B, and 770 : Potent Antitumour Agents from the Caribbean Tunicate Ecteinascidia turbinata", vol. 55, pp. 4512-4515 ; Wright et al., J. Org. Chem., 1990,"Antitumour Tetrahydroisoquinoline Alkaloids from the Colonial Ascidian Ecteinascidia turbinata", vol. 55, pp. 4508-4512 ; Sakai et al., Proc. Natl. Acad. Sci. USA 1992,"Additional antitumour ecteinascidins from a Caribbean tunicate : Crystal structures and activities in vivo", vol. 89, 11456-11460 ; Science 1994,"Chemical Prospectors Scour the Seas for Promising Drugs", vol. 266, pp. 1324 ; Koenig, K. E.,"Asymmetric Synthesis", ed. Morrison, Academic Press, Inc., Orlando, FL, vol. 5, 1985, p. 71 ; Barton, et al., J. Chem Soc. Perkin Trans., 1, 1982,"Synthesis and Properties of a Series of Sterically Hindered Guanidine Bases", pp. 2085 ; Fukuyama et al., J. Am Chem. Soc., 1982,"Stereocontrolled Total Synthesis of (+)-Saframycin B", vol. 104, pp. 4957 ; Fukuyama et al., J. Am Chem Soc., 1990,"Total Synthesis of (+)-Saframycin A", vol. 112, p. 3712 ; Saito, et al., J. Org. Chem., 1989,"Synthesis of Saframycins. Preparation of a Key Tricyclic Lactam Intermediate to Saframycin A", vol. 54, 5391 ; Still, et al., J. Org. Chem., 1978,"Rapid Chromatographic Technique for Preparative Separations with Moderate Resolution", vol. 43, p. 2923 ; Kofron, W. G. ; Baclawski, L. M., J. Org. Chem., 1976, vol. 41, 1879 ; Guan et al., J. Biomolec. Struc. & Dynam., vol. 10 pp. 793-817 (1993) ; Shamma et al.,"Carbon- 13 NMR Shift Assignments of Amines and Alkaloids", p. 206 (1979) ; Lown et al., Biochemistry, 21, 419-428 (1982) ; Zmijewski et al., Chem. Biol. Interactions, 52, 361- 375 (1985) ; Ito, CRC CRIT. Rev. Anal. Chem., 17, 65-143 (1986) ; Rinehart et al., "Topics in Pharmaceutical Sciences 1989"pp. 613-626, D. D. Breimer, D. J. A. Cromwelin, K. K. Midha, Eds., Amsterdam Medical Press B. V., Noordwijk, The Netherlands (1989) ; Rinehart et al.,"Biological Mass Spectrometry,"233-258 eds.

Burlingame et al., Elsevier Amsterdam (1990) ; Guan et al., Jour. Biomolec. Struct. & Dynam., vol. 10 pp. 793-817 (1993) ; Nakagawa et al., J. Amer. Chem. Soc., Ill : 2721- 2722 (1989) ; Lichter et al.,"Food and Drugs from the Sea Proceedings" (1972), Marine Technology Society, Washington, D. C. 1973, 117-127 ; Sakai et al., J. Amer. Chem. Soc. 1996, 118, 9017 ; Garcia-Rocha et al., Brit. J. Cancer, 1996, 73 : 875-883 ; and Pommier et al., Biochemistry, 1996, 35 : 13303-13309 ; Rinehart, K. L., Med. Res. Rev., 2000, 20, 1-27 and I. Manzanares et al, Org. Lett., 2000, 2 (16), 2545-2548.

The most promising ecteinascidin is ecteinascidin 743, which is undergoing clinical trials for treatment of cancers. Et 743 has a complex tris (tetrahydroisoquinolinephenol) structure of the following formula (I) : It is currently prepared by isolation from extracts of the marine tunicate Ecteinascidin turbinata. The yield is low, and alternative preparative processes have been sought.

The ecteinascidins include a fused system of five rings (A) to (E) as shown in the following structure of formula (XIV) : In ecteinascidin 743, the 1, 4 bridge has the structure of formula (IV) : Other known ecteinascidins include compounds with a different bridged cyclic ring system, such as occurs in ecteinascidin 722 and 736, where the bridge has the structure of formula (V) : ecteinascidins 583 and 597, where the bridge has the structure of formula (VI) : and ecteinascidin 594 and 596, where the bridge has the structure of formula (VII) : The complete structure for these and related compounds is given in J. Am. Chem.

Soc. (1996) 118, 9017-9023.

Further compounds are known with the fused five ring system. In general, they lack the bridged cyclic ring system which is present in the ecteinascidins. They include the bis (tetrahydroisoquinolinequinone) antitumor-antimicrobial antibiotics safracins and saframycins, and the marine natural products renieramicins and xestomycin isolated from cultured microbes or sponges. They all have a common dimeric tetrahydroisoquinoline carbon framework. These compounds can be classified into four types, types I to IV, with respect to the oxidation pattern of the aromatic rings.

Type I, dimeric isoquinolinequinones, is a system of formula (VIII) most commonly occuring in this class of compounds, see the following table I.

Table I Structure of Type I Saframycin Antibiotics Substituents Compound R14a R14b R21 R25a R25b R25c <BR> <BR> <BR> saframycin A H H CN O O CH3<BR> <BR> <BR> <BR> <BR> <BR> <BR> saframycin B H H H O O CH3<BR> <BR> <BR> <BR> <BR> <BR> saframycinC H OCH3 H O O CH3 saframycin G H OH CN O O CH3 <BR> <BR> <BR> saframycin H H H CN OH CH2COCH3 CH3<BR> <BR> <BR> <BR> <BR> <BR> saframycin S H H OH O O CH3<BR> <BR> <BR> <BR> <BR> <BR> <BR> saframycin Y3 H H CN NH2 H CH3 saframyin Yd1 H H CN NH2 H C2H5 saframycin AD1 H H CN O O C2H5 <BR> <BR> <BR> <BR> saframycin Yd2 H H CN NH2 H H<BR> <BR> <BR> <BR> <BR> <BR> saframycin Y2b H Q CN NH2 H CH3<BR> <BR> <BR> <BR> <BR> <BR> <BR> saframycin Y2b-d H Q CN NH2 H C2H5 safamycin AH2 H H CN Ha OHa H3 <BR> <BR> <BR> <BR> saframycin AH2Ac H H CN H OAc CH3 safframycin AH1 H H CN OHa Ha CH3 <BR> <BR> <BR> saframycin AHIAc H H CN OAc H CH3<BR> <BR> <BR> <BR> <BR> <BR> <BR> saframycin AR3 H H H H OH CH3 ° assignments are interchangeable. b where the group Q is of formula (IX) : Type I aromatic rings are seen in saframycins A, B and C ; G and H ; and S isolated from Streptomyces lavendulae as minor components. A cyano derivative of saframycin A, called cyanoquinonamine, is known from Japanese Kokai JP-A2 59/225189 and 60/084288. Saframycins Y3, Ydl, Ad, and Yd2 were produced by S. lavendulae by directed biosynthesis, with appropriate supplementation of the culture medium. Saframycins Y2b and Y2b-d dimers formed by linking the nitrogen on the C-25 of one unit to the C-14 of the other, have also been produced in supplemented culture medium of S. lavendulae. Saframycins ARt (=AH2), a microbial reduction product of saframycin A at C-25 produced by Rhodococcus amidophilus, is also prepared by nonstereoselective chemical reduction of saframycin A by sodium borohydride as a 1 : 1 mixture of epimers followed by chromatographic separation (the other isomer AHI is less polar). The further reduction product saframycin AR3, 21-decyano-25-dihydro- saframycin A, (= 25-dihydrosaframycin B) was produced by the same microbial conversion. Another type of microbial conversion of saframycin A using a Nocardia species produced saframycin B and further reduction by a Mycobacterium species produced saframycin AHIAc. The 25-0-acetates of saframycin AH2 and AH, have also been prepared chemically for biological studies.

Type I compounds of formula (X) have also been isolated from marines sponges, see Table II.

Table II Structures of Type I Compounds from Marine Sponges Substituents R"a R"R R renieramycin A OH H H-C (CH3) =CH-CH3 renieramycin B OC2H5 H H-C (CH3) =CH-CH3 renieramycin C OH O O-C (CH3) =CH-CH3 renieramycin D OC2H5 O O-C (CH3) =CH-CH3 renieramycin E H H OH-C (CH3) =CH-CH3 renieramycin F OCH3 H OH-C (CH3) =CH-CH3 xestomycin OCH3 H H-CH3 Renieramycins A-D were isolated from the antimicrobial extract of a sponge, a Reniera species collected in Mexico, along with the biogenetically related monomeric isoquinolines renierone and related compounds. The structure of renieramycin A was initially assigned with inverted stereochemistry at C-3, C-11, and C-13. However, careful examination of the'H NMR data for new, related compounds renieramycins E and F, isolated from the same sponge collected in Palau, revealed that the ring junction of renieramycins was identical to that of saframycins. This result led to the conclusion that the formerly assigned stereochemistry of renieramycins A to D must be the same as that of saframycins.

Xestomycin was found in a sponge, a Xestospongia species collected from Sri Lankan waters.

Type II compounds of formula (XI) with a reduced hydroquinone ring include saframycins D and F, isolated from S. lavendulae, and saframycins Mx-1 and Mx-2, isolated from Myxococcus xanthus. See table III.

Table III Type II Compounds Substituents Compound R R R saframycin D O O H O O CH3 saframycin F O O CN O O CH3 saframycin Mx-1 H OCH3 OH H CH3 NH2 saframycin Mx-2 H OCH3 H H CH3 NH2 The type III skeleton is found in the antibiotics safracins A and B, isolated from cultured Pseudomonasfluorescens. These antibiotics of formula (XII) consist of a tetrahydroisoquinoline-quinone subunit and a tetrahydroisoquinolinephenol subunit.

Where R21 is-H in safracin A and is-OH in safracin B.

Saframycin R, the only compound classified as the Type IV skeleton, was also isolated from S. lavendulae. This compound of formula (XIII), consisting of a hydroquinone ring with a glycolic ester side chain on one of the phenolic oxygens, is conceivably a pro-drug of saframycin A because of its moderate toxicity.

These known compounds include the fused system of five rings of the formula (XIV) : In this text, we refer to this ring structure as the fused ecteinascidin five ring system, though it will be appreciated that the rings A and E are phenolic in the ecteinascidins and some other compounds, while in other compounds, notably the saframycins, the rings A and E are quinolic. In the compounds, the rings B and D are tetrahydro, while ring C is perhydro.

SUMMARY OF THE INVENTION The present invention provides compounds having the fused ecteinascidin five ring system and related to ecteinascidins 583 and 597. In ecteinascidins 583 and 597 the 1, 4 bridge has the structure of formula (VIa) : VIb<BR> VIa Certain compounds of this invention have the fused five ring system of ecteinascidins and the bridge structure of formula (VIa), with the-NH2 optionally derivatised. These compounds can be acylated on the-CHNH2-group present in the formula (VI). Other derivative compounds of this invention comprise those where this- CHNH2-group is replaced by a group-CHNHXI or-C (X2) 2-where Xl or X2 are as defined. The remaining substituents on the fused ecteinascidin five ring system can be the same as those on natural compounds, particularly natural ecteinascidins, or different.

Other compounds of this invention have the fused five ring system of ecteinascidins and the bridge structure of formula (VIb) in which the-NH2 group on the bridge has been replaced with an-OH group which may be optionally derivatised. These compounds can be acylated on the-CHOH-group present in the formula (VIb). Other derivative compounds of this invention comprise those where this-CHOH-group is replaced by a group-CHOX1 or-C (X2) 2-where Xl or X2 are as defined. The remaining substituents on the fused ecteinascidin five ring system can be the same as those on natural compounds, particularly natural ecteinascidins, or different.

In the compounds of this invention, the stereochemistry of the bridgehead carbon atom bearing the-OH or-NH2 group (or substituted derivatives thereof) can be the same as that of the natural compounds, particularly natural ecteinascidins, or different.

In the compounds of this invention, the fused system of five rings (A) to (E) of formula (XIV) can be as in the ecteinascidins, or may be as in other related compounds.

Thus the rings A and E can be phenolic or quinolic ; the rings B and D are tetrahydro, and ring C is perhydro.

Compounds of this invention exhibit antitumor activity, and the invention provides pharamaceutical compositions of the compounds, along with methods for preparing the compositions and methods of treatment using the compounds or compositions.

The invention also provides new hemisynthetic and synthetic routes to the compounds of this invention.

PREFERRED EMBODIMENTS The fused system of five rings (A) to (E) of formula (XIV) is preferably as in the ecteinascidins, and preferably substituted in positions other than 1, 4 with naturally occurring substituents.

In one aspect, the present invention provides new compounds of the formula : wherein : the substituent groups defined by R1, R2 are each independently selected of H, C (=O) R', substituted or unsubstituted Cl-C18 alkyl, substituted or unsubstituted C2-Cl8 alkenyl, substituted or unsubstituted C2-CI8 alkynyl, substituted or unsubstituted aryl ; each of the R'groups is independently selected from the group consisting of H, OH, NO2, NH2, SH, CN, halogen, =O, C (=O) H, C (=O) CH3, C02H, substituted or unsubstituted Cl-Cl8 alkyl, substituted or unsubstituted C2-Cl8 alkenyl, substituted or unsubstituted C2-CI8 alkynyl, substituted or unsubstituted aryl ; X2 is OXO or N (XI) 2 wherein the or each Xi is H, C (=O) R', substituted or unsubstituted Cl-CI8 alkyl, substituted or unsubstituted C2-Clg alkenyl, substituted or unsubstituted C2- Ci8 alkynyl, substituted or unsubstituted aryl, or two Xi groups may together form a cyclic substituent on the nitrogen atom ; X3 is selected of ORI, CN, (=O), or H ; X4 is-H or Cl-Cl8 alkyl ; and X5 is selected of H, OH, or-ORl (wherein OR, is as defined above).

In a related aspect, the invention provides compounds of formula : wherein the substituent groups defined by Rl, R2, X3, X4 and X5 are as defined ; and X is independently selected of H, C (=O) R', substituted or unsubstituted Cl-Cl8 alkyl, substituted or unsubstituted C2-C 18 alkenyl, substituted or unsubstituted C2-Ci8 alkynyl, substituted or unsubstituted aryl, or two Xl groups may together form a cyclic susbstituent on the nitrogen atom.

Alkyl groups preferably have from 1 to about 12 carbon atoms, more preferably 1 to about 8 carbon atoms, still more preferably 1 to about 6 carbon atoms, and most preferably 1, 2, 3 or 4 carbon atoms. Methyl, ethyl and propyl including isopropyl are particularly preferred alkyl groups in the compounds of the present invention. As used herein, the term alkyl, unless otherwise modified, refers to both cyclic and noncyclic groups, although cyclic groups will comprise at least three carbon ring members. The alkyl groups may be straight chain or branched chain.

Preferred alkenyl and alkynyl groups in the compounds of the present invention have one or more unsaturated linkages and from 2 to about 12 carbon atoms, more preferably 2 to about 8 carbon atoms, still more preferably 2 to about 6 carbon atoms, even more preferably 1, 2, 3 or 4 carbon atoms. The terms alkenyl and alkynyl as used herein refer to both cyclic and noncyclic groups, although straight or branched noncyclic groups are generally more preferred.

Preferred alkoxy groups in the compounds of the present invention include groups having one or more oxygen linkages and from 1 to about 12 carbon atoms, more preferably from 1 to about 8 carbon atoms, and still more preferably 1 to about 6 carbon atoms, and most preferably 1, 2, 3 or 4 carbon atoms.

Preferred alkylthio groups in the compounds of the present invention have one or more thioether linkages and from 1 to about 12 carbon atoms, more preferably from 1 to about 8 carbon atoms, and still more preferably 1 to about 6 carbon atoms. Alkylthio groups having 1, 2, 3 or 4 carbon atoms are particularly preferred.

Preferred alkylsulfinyl groups in the compounds of the present invention include those groups having one or more sulfoxide (SO) groups and from 1 to about 12 carbon atoms, more preferably from 1 to about 8 carbon atoms, and still more preferably 1 to about 6 carbon atoms. Alkylsulfinyl groups having 1, 2 3 or 4 carbon atoms are particularly preferred.

Preferred alkylsulfonyl groups in the compounds of the present invention include those groups having one or more sulfonyl (S02) groups and from 1 to about 12 carbon atoms, more preferably from 1 to about 8 carbon atoms, and still more preferably 1 to about 6 carbon atoms. Alkylsulfonyl groups having 1, 2, 3 or 4 carbon atoms are particularly preferred.

Preferred aminoalkyl groups include those groups having one or more primary, secondary and/or tertiary amine groups, and from 1 to about 12 carbon atoms, more preferably 1 to about 8 carbon atoms, still more preferably 1 to about 6 carbon atoms, even more preferably 1, 2, 3 or 4 carbon atoms. Secondary and tertiary amine groups are generally more preferred than primary amine moieties.

Suitable heteroaromatic groups in the compounds of the present invention contain one, two or three heteroatoms selected from N, O or S atoms and include, e. g., coumarinyl including 8-coumarinyl, quinolinyl including 8-quinolinyl, pyridyl, pyrazinyl, pyrimidyl, furyl, pyrrolyl, thienyl, thiazolyl, oxazolyl, imidazolyl, indolyl, benzofuranyl and benzothiazolyl. Suitable heteroalicyclic groups in the compounds of the present invention contain one, two or three heteroatoms selected from N, O or S atoms and include, e. g., tetrahydrofuranyl, tetrahydropyranyl, piperidinyl, morpholino and pyrrolidinyl groups.

Suitable carbocyclic aryl groups in the compounds of the present invention include single and multiple ring compounds, including multiple ring compounds that contain separate and/or fused aryl groups. Typical carbocyclic aryl groups contain 1 to 3 separate or fused rings and from 6 to about 18 carbon ring atoms. Specifically preferred carbocyclic aryl groups include phenyl including substituted phenyl such as 2-substituted phenyl, 3- substituted phenyl, 2, 3-substituted phenyl, 2, 5-substituted phenyl, 2, 3, 5-substituted and 2, 4, 5-substituted phenyl, including where one or more of the phenyl substituents is an electron-withdrawing group such as halogen, cyano, nitro, alkanoyl, sulfinyl, sulfonyl and the like ; naphthyl including 1-naphthyl and 2-naphthyl ; biphenyl ; phenanthryl ; and anthracyl.

Substituent groups defined by Rl, R2, Xl, X4 and X5 are each independently selected from the group consisting of H, OH, OR', SH, SR', SOR', S02R, NO2, NH2, NHR', N (R') 2 NHC (O) R', CN, halogen, =O, substituted or unsubstituted Cl-C18 alkyl, substituted or unsubstituted C2-Cl8 alkenyl, substituted or unsubstituted C2-Cl8 alkynyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaromatic.

References herein to substituted R'groups in the compounds of the present invention refer to the specified moiety that may be substituted at one or more available positions by one or more suitable groups, e. g., halogen such as fluoro, chloro, bromo and iodo ; cyano ; hydroxyl ; nitro ; azido ; alkanoyl such as a fluoro, chloro, bromo and iodo : cyano ; hydroxyl ; nitro ; azido ; alkanoyl such as a C1-6 alkanoyl group such as acyl and the like ; carboxamido ; alkyl groups including those groups having 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms and more preferably 1-3 carbon atoms ; alkenyl and alkynyl groups including groups having one or more unsaturated linkages and from 2 to about 12 carbon or from 2 to about 6 carbon atoms ; alkoxy groups having one or more oxygen linkages and from 1 to about 12 carbon atoms or 1 to about 6 carbon atoms ; aryloxy such as phenoxy ; alkylthio groups including those moieties having one or more thioether linkages and from 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms ; alkylsulfinyl groups including those moieties having one or more sulfinyl linkages and from 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms ; alkylsulfonyl groups including those moieties having one or more sulfonyl linkages and from 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms ; aminoalkyl groups such as groups having one or more N atoms and from 1 to about 12 carbon atoms or from 1 to about 6 carbon atoms ; carbocylic aryl having 6 or more carbons, particularly phenyl (e. g., R being a substituted or unsubstituted biphenyl moiety) ; and aralkyl such as benzyl.

Rl is peferably C (=O) R', where R'is suitably H or substituted or unsubstituted alkyl, more preferably acetyl.

R2 is preferably H or methyl, more preferably methyl.

Typically one of Xl or X2 is often hydrogen. X2, or where permitted Xi is preferably H ;- NHCOalkyl, particularly where the alkyl has up to 16 carbon atoms, such as 1, 4, 7, 15 carbon atoms and may be halosubstituted optionally perhalosubstituted ;-NHalkylCOOH particularly where the alkyl has up to 4 carbon atoms ; protected-NHCOCH (NH2) CH2SH where the NH2 and/or the SH are protected ;-NHbiotin ;-NHaryl ;-NH (aa) y where aa is an amino acid acyl and y is suitably 1, 2 or 3 and wherein any NH2 is optionally derivatised or protected, as with an amide terminal group or a Boc group ; phthalimido formed-NX2- ; alkyl preferably having 1 to 4 carbon atoms ; arylalkenyl, especially cinnamoyl which may be substituted as with 3-trifluoromethyl ; Preferred examples of the group X2 include NHAc, NHCO (CH2) 2COOH, NHCOCH (NHAlloc) CH2SFm, NHCO (CH2) l4CH3, NHTFA, NHCO (CH2) 2CH3, NHCOCH2CH (CH3) 2, NHCO (CH2) 6CH3, NHBiotin, NHBz, NHCOCinn, NHCO- (p- F3C)-Cinn, NHCOVal-NH2, NHCOVal-N-Ac, NHCOVal-N-COCinn, NHCOVal-Ala- NH2, NHCOVal-Ala-N-Ac, NHCOAla-NH2, OH, OAc, NHAc, NHCO (CH2) 2COOH, NHCOCH (NHAlloc) CH2SFm, NHCOCH (NH2) CH2SFm, NPht, NH-(m-CO2Me)-Bz, NHCO (CH2) 14CH3, NMe2, NHTFA, NHCO (CH2) 2CH3, NHCOCH2CH (CH3) 2, NHCO (CH2) 6CH3, NHAlloc, NHTroc, NHBiotin, NHBz, NHCOCinn, NHCO- (p-F3C)- Cinn, NHCOVal-NH2, NHCOVal-N-Ac, NHCOVal-N-COCinn, NHCOVal-Ala-NH2, NHCOVal-Ala-N-Ac, NHCOVal-Ala-N-COCinn, NHCOAla-NH2, NHCOAla-N-Ac, NHCOAla-N-COCinn, OH, OAc, NHAc, NHCO (CH2) 2COOH, NHCOCH (NHAlloc) CH2SFm, Npht, along with similar groups where the number of carbon atoms is varied or the amino acid is changed or another change of this kind is made to give a similar group.

Other preferred examples of the group X2 include OH, OAc, OCOCF3, OCOCH2CH2CH3, OCO (CH2) 6CH3, OCO (CH2) 14CH3, OCOCH=CHPh, OSO2CH3 along with similar groups where the number of carbon atoms is varied or different substituent groups are introduced or another change of this kind is made to give a similar group.

X3 is preferably OH or CN.

X4 is H or Me, preferably Me.

X5 is H or Cl l8 alkyl, preferably H.

In a further, more general aspect of this invention, the compounds are typically of the formula (XVIIa) : or formula (XVIIb) : where R'and R4 together form a group of formula (VIa) or (VIb) : Vla Vlb R is-H or-OH ; R is-OCH3 and R8 is-OH or R and R8 together form a group-0-CH2-0- ; R14a and R14b are both-H or one is-H and the other is-OH,-OCH3 or-OCH2CH3, or Rl4a and Rab together form a keto group ; and Ris is-H or-OH ; R21 is-H,-OH or-CN ; and derivatives including acyl derivatives thereof especially where R5 is acetyloxy or other acyloxy group of up to 4 carbon atoms, and including derivatives where the group -NCH3-at the 12-position is replaced by-NH-or-NCH2CH3-, and derivatives where the- NH2 group in the compound of formula (VIa) and the-OH group in the compound of formula (VIb) are optionally derivatised.

The group R1 with R4 can be acylated on the-CHNH2-or-CHOH-group present in the formulae (VIa and VIb). Other derivative compounds of this invention comprise those where the-CHNH2 group of VIa is replaced by a group-CHNHXl or- C (X2) 2- or where the-CHOH group of VIb is replaced by CHOUX, or-C (X2) 2- where Xi or X2 are as defined.

Preferred compounds are of the formula (XVIIb).

Furthermore, in preferred compounds of this invention, R7 and R8 together form a group-O-CH2-O-.

The acyl derivatives can be N-acyl or N-thioacyl derivatives thereof, as well as cyclic amides. The acyl groups can illustratively be alkanoyl, haloalkanoyl, arylalkanoyl, alkenyl, heterocyclylacyl, aroyl, arylaroyl, haloaroyl, nitroaroyl, or other acyl groups. The acyl groups can be of formula-CO-Ra, where Ra can be various groups such as alkyl, alkoxy, alkylene, arylalkyl, arylalkylene, amino acid acyl, or heterocyclyl, each optionally substituted with halo, cyano, nitro, carboxyalkyl, alkoxy, aryl, aryloxy, heterocyclyl, heterocycyloxy, alkyl, amino or substituted amino. Other acylating agents include isothiocyanates, such as aryl isothiocyanates, notably phenyl isocyanate. The alkyl, alkoxy or alkylene groups of Ra suitably have 1 to 6 or 12 carbon atoms, and can be linear, branched or cyclic. Aryl groups are typically phenyl, biphenyl or naphthyl.

Hetercyclyl groups can be aromatic or partially or completely unsaturated and suitably have 4 to 8 ring atoms, more preferably 5 or 6 ring atoms, with one or more heteroatoms selected from nitrogen, sulphur and oxygen.

Without being exhaustive, typical Ra groups include alkyl, haloalkyl, alkoxyalkyl, haloalkoxyalkyl, arylalkylene, haloalkylarylakylene, acyl, haloacyl, arlyalkyl, alkenyl and amino acid. For example, Ra-CO-can be acetyl, trifluoroacetyl, 2, 2, 2- trichloroethoxycarbonyl, isovalerylcarbonyl, trans-3- (trifluoromethyl) cinnamoylcarbonyl, heptafluorobutyrylcarbonyl, decanoylcarbonyl, trans-cinnamoylcarbonyl, butyrylcarbonyl, 3-chloropropyonylcarbonyl, cinnamoylcarbonyl, 4- methylcinnamoylcarbonyl, hydrocinnamoylcarbonyl, or trans-hexenoylcarbonyl, or alanyl, arginyl, aspartyl, asparagyl, cystyl, glutamyl, glutaminyl, glycyl, histidyl, hydroxyprolyl., isoleucyl, leucyl, lysyl, methionyl, phenylalanyl, prolyl, seryl, threonyl, thyronyl, tryptophyl, tyrosyl, valyl, as well as other less common amino acid acyl groups, as well as phthalimido and other cyclic amides. Other examples may be found among the listed protecting groups.

Compounds wherein-CO-Ra is derived from an amino acid and include an amino group can themselves form acyl derivatives. Suitable N-acyl commands include dipeptides which in turn can form N-acyl derivatives.

Preferably Rlaa and Rlab are hydrogen. Preferably Rl5 is hydrogen. The O-acyl derivatives are suitably aliphatic O-acyl derivatives, especially acyl derivatives of 1 to 4 carbon atoms, and typically an 0-acetyl group, notably at the 5-position.

Suitable protecting groups for phenols and hydroxy groups include ethers and esters, such as alkyl, alkoxyalkyl, aryloxyalkyl, alkoxyalkoxyalkyl, alkylsilylalkoxyalkyl, alkylthioalkyl, arylthioalkyl, azidoalkyl, cyanoalkyl, chloroalkyl, heterocyclic, arylacyl, haloarylacyl, cycloalkylalkyl, alkenyl, cycloalkyl, alyklarylalkyl, alkoxyarylalkyl, nitroarylalkyl, haloarylalkyl, alkylaminocarbonylarylalkyl, alkylsulfinylarylalkyl, alkylsilyl and other ethers, and arylacyl, aryl alkyl carbonate, aliphatic carbonate, alkylsulfinylarlyalkyl carbonate, alkyl carbonate, aryl haloalkyl carbonate, aryl alkenyl carbonate, aryl carbamate, alkyl phosphinyl, alkylphosphinothioyl, aryl phosphinothioyl, aryl alkyl sulphonate and other esters. Such groups may optionally be substituted with the previously mentioned groups in Rl.

Suitable protecting groups for amines include carbamates, amides, and other protecting groups, such as alkyl, arylalkyl, sulpho-or halo-arylalkyl, haloalkyl, alkylsilylalkyl, arylalkyl, cycloalkylalkyl, alkylarylalkyl, heterocyclylalkyl, nitroarylalkyl, acylaminoalkyl, nitroaryldithioarylalkyl, dicycloalkylcarboxamidoalkyl, cycloalkyl, alkenyl, arylalkenyl, nitroarylalkenyl, heterocyclylalkenyl, heterocyclyl, hydroxyheterocyclyl, alkyldithio, alkoxy-or halo-or alkylsulphinyl arylalkyl, hetercyclylacyl, and other carbamates, and alkanoyl, haloalkanoyl, arylalkanoyl, alkenoyl, heterocyclylacyl, aroyl, arylaroyl, haloaroyl, nitroaroyl, and other amides, as well as alkyl, alkenyl, alkylsilylalkoxyalkyl, alkoxyalkyl, cyanoalkyl, heterocyclyl, alkoxyarylalkyl, cycloalkyl, nitroaryl, arylalkyl, alkoxy-or hydroxy-arylalkyl, and many other groups. Such groups may optionally be substituted with the previously mentioned groups in Rl.

Examples of such protecting groups are given in the following tables. protection for-OH group ethers abbreviation methyl methoxymethyl MOM benzyloxymethyl BOM methoxyethoxymethyl MEM 2- (trimethylsilyl) ethoxymethyl SEM methylthiomethyl MTM phenylthiomethyl PTM azidomethyl cyanomethyl 2, 2-dichloro-1, 1-difluoroethyl 2-chloroethyl 2-bromoethyl tetrahydropyranyl THP 1-ethoxyethyl EE phenacyl 4-bromophenacyl cyclopropylmethyl allyl propargyl isopropyl cyclohexyl t-butyl benzyl 2, 6-dimethylbenzyl 4-methoxybenzyl MPM or PMB o-nitrobenzyl 2, 6-dichlorobenzyl 3, 4-dichlorobenzyl 4- (dimethylamino) carbonylbenzyl 4-methylsuflinylbenzyl Msib 9-anthrylmethyl 4-picolyl heptafluoro-p-tolyl tetrafluoro-4-pyridyl trimethylsilyl TMS t-butyldimethylsilyl TBDMS t-butyldiphenylsilyl TBDPS triisopropylsilyl TIPS esters aryl formate aryl acetate aryl levulinate aryl pivaloate ArOPv aryl benzoate aryl 9-fluorocarboxylate aryl methyl carbonate 1-adamantyl carbonate t-butyl carbonate BOC-OAr 4-methylsulfinylbenzyl carbonate Msz-Oar 2, 4-dimethylpent-3-yl carbonate Doc-Oar aryl 2, 2, 2-trichloroethyl carbonate aryl vinyl carbonate aryl benzyl carbonate aryl carbamate dimethylphosphinyl Dmp-OAr dimethylphosphinothioyl Mpt-OAr diphenylphosphinothioyl Dpt-Oar aryl methanesulfonate aryl toluenesulfonate aryl 2-formylbenzenesulfonate protection for the-NH2 group carbamates abbreviation methyl ethyl 9-fluorenylmethyl Fmoc 9-(2-sulfo) fluroenylmethyl 9- (2, 7-dibromo) fluorenylmethyl 17-tetrabenzo [a, c, g, i] fluorenylmethyl Tbfmoc 2-chloro-3-indenylmethyl Climoc benz [flinden-3-ylmethyl Bimoc 2, 7-di-t-butyl [9- ( 10, 10-dioxo-10, 10, 10, 10- tetrahydrothioxanthyl)] methyl DBD-Tmoc 2, 2, 2-trichloroethyl Troc 2-trimethylsilylethyl Teoc 2-phenylethyl hZ 1- (1-adamantyl)-1-methylethyl Adpoc 2-chlooethyl 1, 1-dimethyl-2-chloroethyl 1, 1-dimethyl-2-bromoethyl 1, 1-dimethyl-2, 2-dibromoethyl DB-t-BOC 1, 1-dimethyl-2, 2, 2-trichloroethyl TCBOC 1-methyl-1- (4-biphenyl) ethyl Bpoc 1- (3, 5-di-t-butylphenyl)-1-1-methylethyl t-Burmeoc 2- (2'-and 4'-pyridyl) ethyl Pyoc 2, 2-bis (4'-nitrophenyl) ethyl Bnpeoc n- (2-pivaloylamino)-1, 1-dimethylethyl 2- [(2-nitrophenyl) dithio]-1-phenylethyl NpSSPeoc 2- (n, n-dicyclohexylcarboxamido) ethyl t-butyl BOC 1-adamantyl 1-Adoc 2-adamantyl 2-Adoc vinyl Voc allyl Aloc or Alloc 1-isopropylallyl Ipaoc cinnamyl Coc 4-nitrocinnamyl Noc 3- (3'-pyridyl) prop-2-enyl Paloc 8-quinolyl n-hydroxypiperidinyl alkyldithio benzyl Cbz or Z p-methoxybenzyl Moz p-nitrobenzyl PNZ p-bromobenzyl p-chlorobenzyl 2, 4-dichlorobenzyl 4-methylsulfinylbenzyl Msz 9-anthrylmethyl diphenylmethyl phenothiazinyl- (10)-carbonyl <BR> <BR> n'-p-toluenesulfonylaminocarbonyl<BR> <BR> n'-phenylaminothiocarbonyl amides formamide acetamide chloroacetamide trifluoroacetamide TFA phenylacetamide 3-phenylpropanamide pent-4-enamide picolinamide 3-pyridylcarboxamide benzamide p-phenylbenzamide n-phthalimide n-tetrachlorophthalimide TCP 4-nitro-n-phthalimide n-dithiasuccinimide Dts n-2, 3-diphenylmaleimide n-2, 5-dimethylpyrrole n-2, 5-bis (triisopropylsiloxyl) pyrrole BIPSOP n-1, 1, 4, 4-tetramethyldisiliazacyclopentante adduct STABASE 1, 1, 3, 3-tetramethyl-1, 3-disilaisoindoline BSB special-NH protective groups n-methylamine n-t-butylamine n-allylamine n- (2-trimethylsilyl) ethoxy] methylamine SEM n-3-acetoxypropylamine n-cyanomethylamine n- (l-isopropyl-4-nitro-2-oxo-3-pyrrolin-3-yl) amine n-2, 4-dimethoxybenzylamine Dmb 2-azanorbornenes n-2, 4-dinitrophenylamine n-benzylamine Bn n-4-methoxybenzylamine MPM n-2, 4-dimethoxybenzylamine DMPM n-2-hydroxybenzylamine Hbn n- (diphenylmethyl) amino DPM n-bis (4-methoxyphenyl) methylamine n-5-dibenzosuberylamine DBS n-triphenylmethylamine Tr n- [ (4-methoxyphenyl) diphenylmethyl] amino MMTr n-9-phenylflurenylamine Pf n-ferrocenylmethylamine Fcm n-2-picolylamine n'-oxide n-1, 1-dimethylthiomethyleneamine n-benzylideneamine <BR> <BR> n-p-methoxybenzylideneamine<BR> <BR> n-diphenylmethyleneamine n- (5, 5-dimethyl-3-oxo-1-cyclohexenyl) amine n-nitroamine n-nitrosoamine diphenylphosphinamide Dpp dimethylthiophosphinamide Mpt diphenylthiophosphinamide Ppt dibenzyl phosphoramidate 2-nitrobenzenesulfenamide Nps n-1- (2, 2, 2-trifluoro-1, 1-diphenyl) ethylsufenamide TDE 3-nitro-2-pyridinesulfenamide Npys p-toluenesulfonamide Ts benzenesulfonamide A preferred class of compounds of this invention include compunds of formula (XVIIb), where one or more, preferably all of the following conditions are met : the amino group in the group of formula (VIa) is derivatised ; the hydroxy group in the group of formula (VIb) is derivatised ; RS is ORI ; R and R8 together form a group-O-CH2-O-; R14a and R14b are both-H ; Rl5 is H ; and/or R21 is-OH or-CN.

Particular ecteinascidin products of this invention include compounds of the formula (XVIII) ; where Rl and R4 form a group of formula (VIa or VIb) : VIb<BR> VIa R21 is-H, -OH or -CN, moe particularly -OH or -CN; and acyl derivatives thereof, more particularly 5-acyl derivatives including the 5-acetyl derivative, and where the-NH2 group in the structure of formula (VIa) and the-OH group in the structure of formula (VIb) are optionally derivatised.

Compounds of the present invention notably with one of two group X can be prepared synthetically from the intermediate compound (47) desribed in the U. S. Patent No 5, 721, 362, or a similar compound. Thus the present invention provides a process which involves derivatisation of the 1, 4 bridge amino group, according to the following reaction scheme : where Xl is as defined, and other substituent groups on the molecule can be protected or derivatised as desired or appropriate.

Compounds of this invention notably with the groups X2 being-OX2 can be prepared from the intermediate compound (15) described in the U. S. Patent No 5, 721, 362 or a similar compound. Thus, the present invention provides a process which involves derivatisation of the 1, 4 bridge amino group, according to the following reaction scheme : where XI is as defined, and other substituent groups on the molecule can be protected or derivatised as desired or appropriate. The reaction may proceed with formation of a substituent-OX where Xi is hydrogen, and then conversion to a compound where Xi is another group.

It will be apparent that compounds of this invention may also be prepared by modification of the synthetic steps employed in the U. S. Patent No 5, 721, 362. Thus, for instance, different reactive groups may be introduced at functional positions, for example at the 5-or 18-positions.

A more general route to compounds if this invention is provided, and was first disclosed in WO 00/69862, incorporated herein in full by reference and from which priority is claimed.

A typical process of that WO application concerns method for preparing a compound with a fused ring structure of formula (XIV) : which comprises one or more reactions starting from a 21-cyano compound of formula (XVI) : where : R'is an amidomethylene group or an acyloxymethylene group ; R and R8 are independently chosen from-H,-OH or-OCOCH20H, or Rs and R8 are both keto and the ring A is ap-benzoquinone ring ; Rl4a and Rab are both-H or one is-H and the other is-OH,-OCH3 or-OCH2CH3 or Riva and Rl4b together form a keto group ; and Ris and Rl8 are independently chosen from-H or-OH, or Rs and R are both keto and the ring A is ap-benzoquinone ring.

In particular, such a method can provide a route to the starting materials for the reactions of Schemes I and II, along with related compounds.

Antitumoural activities of these compounds include leukaemias, lung cancer, colon cancer, kidney cancer, prostate cancer, ovarian cancer, breast cancer, sarcomas and melanomas.

Another especially preferred embodiment of the present invention is pharmaceutical compositions useful as antitumour agents which contain as active ingredient a compound or compounds of the invention, as well as the processes for their preparation.

Examples of pharmaceutical compositions include any solid (tablets, pills, capsules, granules etc.) or liquid (solutions, suspensions or emulsions) with suitable composition or oral, topical or parenteral administration.

Administration of the compounds or compositions of the present invention may be any suitable method, such as intravenous infusion, oral preparation, intraperitoneal and intravenous preparation.

For the avoidance of doubt, the stereochemistries indicated in this patent specification are based on our understanding of the correct stereochemistry of the natural products. To the extent that an error is discovered in the assigned stereochemistry, then the appropriate correction needs to be made in the formulae given throughout in this patent specification. Furthermore, to the extent that the syntheses are capable of modification, this invention extends to stereoisomers.

DETAILED DESCRIPTION OF PREFERRED PROCESSES The compounds of the present invention can be synthetically prepared from the intermediate compounds 47 and 15 described in the U. S. Patent No 5, 721, 362, the compound 36 described in WO 00/69862 and from the secondary products (numbered here as 23 and 24) obtained in some deprotection steps using Aids of the compound 33 of WO 00/69862.

(23) (24) Compound (1) corresponds to the synthetic intermediate (47) described in the US patent No 5, 721, 362. Compounds 27 and 28 included in Table IV are described as 35 and 34 in WO 00/69862.

Some of the preferred methods of producing the compound of formula I are described below in the following reaction schemes with examples of typical substituent groups.

These typical substituents are not limiting of the invention, and the process is to be understood in the more general sense, without special regard to the identities indicated by the code letters.

Numerous active antitumoral compounds have been prepared from this compounds and it is believed that many more compounds can be formed in accordance with the teachings of the present disclosure.

SCHEME I R : a : AcNH- b : F3CCONH- c : CH3 (CH2) 2CONH- d : (CH3) 2CHCH2CONH- e : CH3 (CH2) 6CONH- f : CH3 (CH2) I4CONH- g : BzNH- h : CinnCONH- 5 i : p-F3C-CinnCONH- j : PhtN- k : BiotinCONH- 1 : HO2CCH2CH2CONH- m : (CH3) 2N- n : BnNH- o : PrNH- Cinn : SCHEME II R : p : NH2-ValCONH- q : Ac-N-ValCONH- r : CinnCO-N-ValCONH- s : NH2-Ala-ValCONH- t : Ac-N-Ala-ValCONH- u : CinnCO-N-Ala-ValCONH- v : NH2-AlaCONH- w : Ac-N-AIaCONH- x : CinnCO-N-AlaCONH- y : FmSCH2CH (NHAlloc) CONH- z : FmSCH2CH (NH2) CONH- 7 : Boc-N-ValCONH- 8 : Boc-N-AlaCONH- 9 : Boc-N-Ala-ValCONH SCHEME III 19, 21a, 21c, 21e-f, 21h, 2111, 22a* R" : a : Ac- b : F3CCO- c : CH3 (CH2) 2CO- e : CH3 (CH2) 6CO- f : CH3 (CH2) z4CO- 15, 16*, 17a-c, 17e-f, 17h, 1711, 18a* h : CinnCO- 11 : MeSO2- 15 : H- 16 : H- 19 : H- Cinn : SCHEME IV R, X2 23 H Me 24 Ac H R, X2 --25 H Me 26 Ac H The type of reactions are the following : Methods A, B, C, E and M include different acylation methods with acid chlorides, anhydrides, acids or sulfonyl chlorides, to obtain amide or ester bonds.

Methods D and H involve reductive alkylation reactions between an aldehyde and 1 or an amine and 5 to give 2m or 3o.

Method F transforms compound 1 to 2n by reaction with BnBr and Cs2CO3.

Method G involves the deprotection of methoxymethyl group (MOM) or MOM/tert- butyloxy carbonyl groups or MOM/allyloxy carbonyl groups using trimethylchlorosilane (TMSCI) and sodium iodide.

Methods I (AgN03) and J (CuBr) convert CN into OH in position C-21.

Method K involves the hydrolysis of a carbamate bond using aqueous trifluoroacetic acid.

Method L converts a carbonyl group to an alcohol by reduction with NaCNBH3 in the presence of acetic acid. With this reaction a new chiral center is generated. Taking into account steric effects and spectroscopic data, it seem that the main compound (11) has R configuration at this center and the secondary product (12*) has S configuration. On this basis 13, 15, 17, 19, 21 will have R configuration and 14*, 18* and 22* will have S configuration. These assignments have been made based on the available spectral data and as such, in the absence of specific studies to confirm the assignments, should be considered as only tentative.

Modified processes can be used to prepare other compounds of this invention. In particular the starting material and/or reagents and reactions can be varied to suit other combinations of the substituent groups.

In another aspect, the present invention is directed at the use of a known compound, safracin B, also referred to as quinonamine, in hemisynthetic synthesis.

More generally, the invention relates to a hemisynthetic process for the formation of intermediates, derivatives and related structures of ecteinascidin or other tetrahydroisoquinolinephenol compounds starting from natural bis (tetrahydroisoquinoline) alkaloids.

Suitable preferred starting materials for the hemi-synthetic process include the classes of saframycin and safracin antibiotics available from different culture broths, and also the classes of reineramicin and xestomycin compounds available from marine sponges.

A general formula (XV) for the starting compounds is as follows : where : R'is an amidomethylene group such as-CH2-NH-CO-CR25aR25bR25c where R25a and R21b form a keto group or one is-OH,-NH2 or-OCOCH3 and the other is-CH2COCH3,-H,- OH or-OCOCH3, provided that when R25a is-OH or-NH2 then R25b is not-OH, and R25c is-H,-CH3 or-CH2CH3, or R1 is an acyloxymethylene group such as-CH2-O-CO-R, where R is-C (CH3) =CH-CH3 or-CH3 ; R and R are independently chosen from-H,-OH or-OCOCH20H, or R5 and R8 are both keto and the ring A is a p-benzoquinone ring ; R'and R are both-H or one is-H and the other is-OH,-OCH3 or-OCH2CH3, or Rua and R'4b together form a keto group ; Rl5 and Rl8 are independently chosen from-H or-OH, or Rs and R8 are both keto and the ring A is a p-benzoquinone ring ; and R2'is-OH or-CN.

A more general formula for these class of compounds is provided below : wherein the substituent groups defined by Ri, R2, R3, R4, R5, R6, R7, R8, R9, Rlo are each independently selected from the group consisting of H, OH, OCH3, CN, =O, CH3 ; wherein X are the different amide or ester functionalities contained in the mentioned natural products ; wherein each dotted circle represents one, two or three optional double bonds.

Thus, according to the present invention, we now provide hemisynthetic routes for the production of new and known compounds. The hemisynthetic routes of the invention each comprise a number of transformation steps to arrive at the desired product. Each step in itself is a process in accordance with this invention. The invention is not limited to the routes that are exemplified, and alternative routes may be provided by, for example, changing the order of the transformation steps, as appropriate.

In particular, this invention involves the provision of a 21-cyano starting material of general formula (XVI) : where R I, R5, R8, R14a, R141, R"and R"are as defined.

Other compounds of formula (XVI) with different substituents at the 21-position may also represent possible starting materials. In general, any derivative capable of production by nucleophilic displacement of the 21-hydroxy group of compounds of formula (XV) wherein R21 is a hydroxy group is a candidate. Examples of suitable 21- substituents include but are not limited to : a mercapto group ; an alkylthio group (the alkyl group having from 1 to 6 carbon atoms) ; an arylthio group (the aryl group having from 6 to 10 carbon atoms and being unsubstituted or substituted by from 1 to 5 substituents selected from, for example, alkyl group having from 1 to 6 carbon atoms, alkoxy groups having from 1 to 6 carbon atoms, halogen atoms, mercapto groups and nitro groups) ; an amino group ; a mono-or dialkylamino (the or each alkyl group having from 1 to 6 carbon atoms) ; a mono-or diarylamino group (the or each aryl group being as defined above in relation to arylthio groups) ; an a-carbonylalkyl group of formula-C (Ra) (Rb)-C (=O) R, where Ra and Rb are selected from hydrogen atoms, alkyl groups having from 1 to 20 carbon atoms, aryl groups (as defined above in relation to arylthio groups) and aralkyl groups (in which an alkyl group having from 1 to 4 carbon atoms is substituted by an aryl group a defined above in relation to arylthio groups), with the proviso that one of Ra and Rb is a hydrogen atom ; Rc is selected from a hydrogen atom, an alkyl group having from 1 to 20 carbon atoms, aryl groups (as defined above in relation to arylthio groups), an aralkyl group (in which an alkyl group having from 1 to 4 carbon atoms is substituted by an aryl group a defined above in relation to arylthio groups), an alkoxy group having from 1 to 6 carbon atoms, an amino group or a mono-or dialkylamino group as defined above.

Thus, in a more general aspect, the present invention relates to processes where the first step is to form a 21-deriviative using a nucleophilic reagent. We refer to such compounds as 21-Nuc compounds. Preferred starting material 21-Nuc compounds have the structure of formula (XIV) : where at least one ring A or E is quinolic.

Thus, in addition to the use of 21-cyano compounds, processes using other nucleophile-containing compounds, to produce similar compounds of formula (XVI) wherein the 21-position is protected by another nuclephilic group, a 21-Nuc group, are also envisaged. For example, a 21-Nuc compound of formula (XVI) with an alkylamino substituent at the 21-position can be produced by reacting the compound of formula (XV) wherein R2'is a hydroxy group with a suitable alkylamine. A 21-Nuc compound of formula (XVI) with an alkylthio substituent at the 21-position can also be produced by reacting the compound of formula (XV) wherein R2'is a hydroxy group with a suitable alkanethiol. Alternatively, a 21-Nuc compound of formula (XVI) with an a-carbonylalkyl substituent at the 21-position can be produced by reacting the compound of formula (XV) wherein R21 is a hydroxy group with a suitable carbonyl compound, typically in the presence of a base. Other routes are available for other 21-Nuc compounds.

The presence of the 21-cyano group is required for some of the end-products, notably ecteinascidin 770 and phthalascidin, while for other end-products it acts as a protecting group which can readily be converted to another substituent, such as the 21- hydroxy group. The adoption of the 21-cyano compound as the starting material effectively stabilises the molecule during the ensuing synthetic steps, until it is optionally removed. Other 21-Nuc compounds can offer this and other advantages.

Preferred starting materials include those compounds of formula (XV) or (XVI) where R and Rl4b are both hydrogen. Preferred starting materials also include compounds of formula (XV) or (XVI) where Rl5 is hydrogen. Furthermore, the preferred starting materials include compounds of formula (XV) or (XVI) where ring E is a phenolic ring. Preferred starting materials further include compounds of formula (XV) or (XVI) where at least one, better at least two or three of R5, R8, Rl5 and Rl8 is not hydrogen.

Examples of suitable starting materials for this invention include saframycin A, saframycin B, saframycin C, saframycin G, saframycin H, saframycin S, saframycin Y3, saframycin Ydl, saframycin Ad,, saframycin Yd2, saframycin AH2, saframycin AH2Ac, saframycin AH1, saframycin AH, Ac, saframycin AR3, renieramycin A, renieramycin B, renieramycin C, renieramycin D, renieramycin E, renieramycin F, xestomycin, saframycin D, saframycin F, saframycin Mx-1, saframycin Mx-2, safracin A, safracin B and saframycin R. Preferred starting materials have a cyano group in position 21, for the group R. 1.

In a particularly preferred aspect, the invention involves a hemisynthetic process wherein the transformation steps are applied to safracin B : SAFRACIN B Safracin B presents a ring system closely related to the ecteinascidins. This compound has the same pentacycle structure and the same substitution pattern in the right-hand aromatic ring, ring E.

The more preferred starting materials of this invention have a 21-cyano group.

The currently most preferred compound of the present invention is the compound of Formula 2. This compound is obtained directly from safracin B and is considered a key intermediate in the hemisynthetic process. compound 2 Cyanosafracin B by fermentation of a safracin B-producing strain of Pseudomonasfluorescens, and working up the cultured broth using cyanide ion. The preferred strain of Pseudomonasfluorescens is strain A2-2, FERM BP-14, which is employed in the procedure of EP-A-055 299. A suitable source of cyanide ion is potassium cyanide. In a typical work-up, the broth is filtered and excess cyanide ion is added. After an appropriate interval of agitation, such as 1 hour, the pH is rendered alkaline, say pH 9. 5, and an organic extraction gives a crude extract which can be further purified to give the cyanosafracin B.

In general, the conversion of the 21-cyano starting compound to an product of this invention involves : a) conversion if necessary of a quinone system for the ring E into the phenol system b) conversion if necessary of a quinone system for the ring A into the phenol system ; c) conversion of the phenol system for the ring A into the methylenedioxyphenol ring ; d) formation of the bridged spiro ring system of formula (IV), (VI) or (VII) across the 1-position and 4-position in ring B ; and e) derivatisation as appropriate, such as acylation.

Step (a), conversion if necessary of a quinone system for the ring E into the phenol system, can be effected by conventional reduction procedures. A suitable reagent system is hydrogen with a palladium-carbon catalyst, though other reducing systems can be employed.

Step (b), conversion if necessary of a quinone system for the ring A into the phenol system is analogous to step (a), and more detail is not needed.

Step (c), conversion of the phenol system for the ring A into the methylenedioxyphenol ring, can be effected in several ways, possibly along with step (b).

For example, a quinone ring A can be demethylated in the methoxy substituent at the 7- position and reduced to a dihydroquinone and trapped with a suitable electrophilic reagent such as CH2Br2, BrCH2Cl, or a similar divalent reagent directly yielding the methylenedioxy ring system, or with a divalent reagent such as thiocarbonyldiimidazol which yields a substituted methylenedioxy ring system which can be converted to the desired ring.

Step (d) is typically effected by appropriate substitution at the 1-position with a bridging reagent that can assist formation of the desired bridge, forming an exendo quinone methide at the 4-position and allowing the methide to react with the 1-substituent to bring about the bridged structure. Preferred bridging reagents are of formula (XIX) where Fu indicates a protected functional group, such as a group-NHProt4a or OProt4b, Prot3 is a protecting group, and the dotted line shows an optional double bond.

Suitably the methide is formed by first introducing a hydroxy group at the 10- position at the junction of rings A and B to give a partial structure of formula (XX) : or more preferably a partial structure of formula (XXI) : where the group R"is chosen for the desired group of formula (IV), (V), (VI) or (VII).

For the first two such groups, the group R"typically takes the form-CHFu-CH2-SProt3.

The protecting groups can then be removed and modified as appropriate to give the desired compound. ° A typical procedure for step (d) is provided in US Patent 5, 721, 362 incorporated by reference. Particular reference is made to the passage at column 8, step (1) and Example 33 of the US Patent, and related passages.

Derivatisation in step (e) can include acylation, for instance with a group Ra-CO- as well as conversion of the 12-NCH3 group to 12-NH or 12-NCH2CH3. Such conversion can be effected before or after the other steps, using available methods.

By way of illustration, can be transformed into Intermediate 25 ; INT-25 and from this derivative it is possible to introduce a number of cysteine derivatives that can be transformed into compounds of this invention. Preferred cysteine derivatives are exemplified by the following two compounds : Int-29 Int-37 One method of this invention transforms cyanosafracin B into intermediate Int-25 through a sequence of reactions that involves essentially (1) removal of methoxy group placed in ring A, (2) reduction of ring A and formation of methylene-dioxy group in one pot, (3) hydrolysis of amide function placed over carbon 1, (4) transformation of the resulting amine group into hydroxyl group, see scheme V.

Scheme V OMe OMe Me H, Me H, Me 0 1 0 1 0 OMe Mev 020, EtO H 4 OMBr, DIPEA X Me O N Me O N C HC N Me I I N_ _M e NU NU NHBoc 0 N O non ,-, kNHBoc oJ » N HB o c Me Me e OMe OMe 0 OMe O Me O Me O M M O \ OH \ O/I NaOH 1M Me I I -M e ) , p Me I N _M e Allyl bromide Me N-M e N-M M H H N 2) CIBrCHz, CszCOa O/N C sC O I/N N H N N H N -O N c N -N M o c N HB o c N HB o c Int-17 Int-16 Int-17 OMe Int-18 OMe OMe H/Me O H, I Me H Me mye N--M e Hpioxane 4. 3M N--M e HCIIDioxane 4. 3M Me Phenylisothiocyanate N C HCf ,-c L C O, O N H N O N H N of N hui T pN H In-20 Int-21 Int-19 The method avoids protection and de-protection of the primary alcohol function at the position 1 in ring B of compound Int-25 using directly a cysteine residue Int-29 to form intermediate Int-27. Cysteine derivative Int-29 is protected in the amino group with P-p-p-trichloroethoxycarbonyl protecting group in order to have compatibility with the existing allyl and MOM groups. Intermediate Int-27 is directly oxidized and cycled.

These circumstances, together with a different de-protecting strategy in the later stages of the synthesis makes the route novel and more amenable to industrial development than the process of US 5, 721, 362.

The conversion of the 2-cyano compound into Intermediate Int-25 usually involves the following steps (see scheme V) : formation of the protected compound of Formula Int-14 by reacting Int-2 with tert- butoxycarbonyl anhydride ; converting of Int-14 into the di-protected compound of Formula Int-15 by reacting with bromomethylmethyl ether and diisopropylethylamine in acetonitrile ; selective elimination of the methoxy group of the quinone system in Int-15 to obtain the compound of Formula Int-16 by reacting with a methanolic solution of sodium hydroxide ; transforming of Int-16 into the methylene-dioxy compound of Formula Int-18 by employing the next preferred sequence : (1) quinone group of compound Int-16 is reduced with 10% Pd/C under hydrogen atmosphere ; (2) the hydroquinone intermediate is converted into the methylenedioxy compound of Formula Int-17 by reacting with bromochloromethane and caesium carbonate under hydrogen atmosphere ; (3) Int-17 is transformed into the compound of Formula Int-18 by protecting the free hydroxyl group as a OCH2R group. This reaction is carried out with BrCH2R and caesium carbonate, where R can be aryl, CH=CH2, OR'etc. elimination of the tert-butoxycarbonyl and the methyloxymethyl protecting groups of Int-18 to afford the compound of Formula Int-19 by reacting with a solution of HCl in dioxane. Also this reaction is achieved by mixing Int-18 with a solution of trifluoroacetic acid in dichloromethane ; formation of the thiourea compound of Formula Int-20 by reacting Int-19 with phenylisothiocyanate ; converting compound of Formula Int-20 into the amine compound of Formula Int-21 by reacting with a solution of hydrogen chloride in dioxane ; transforming compound of Formula Int-21 into the N-Troc derivative Int-22 by reacting with trichloroethyl chloroformate and pyridine ; formation of the protected hydroxy compound of Formula Int-23 by reacting Int-22 with bromomethylmethyl ether and diisopropylethylamine ; transforming compound of Formula Int-23 into the N-H derivative Int-24 by reacting with acetic acid and zinc ; conversion of compound of Formula Int-24 into the hydroxy compound of Formula Int- 25 by reaction with sodium nitrite in acetic acid. Alternatively, it can be used nitrogen tetroxide in a mixture of acetic acid and acetonitrile followed by treatment with sodium hydroxide. Also, it can be used sodium nitrite in a mixture of acetic anhydride-acetic acid, followed by treatment with sodium hydroxide.

From intermediate Int-25 the conversion into final intermediate compounds Int- 35 or Int-36 of this invention can then proceed as shown in Scheme VI : Scheme VI transforming compound of formula Int-24 into the derivative Int-30 by protecting the primary hydroxyl function with (S)-N-2, 2, 2-tricloroethoxycarbonyl-S- (9H-fluoren-9- ylmethyl) cysteine Int-29 ; converting the protected compound of formula Int-30 into the phenol derivative Int-31 by cleavage of the allyl group with tributyltin hydride and dichloropalladium-bis (triphenylphosphine) ; transforming the phenol compound of Formula Int-31 into compound of formula Int-32 by oxidation with benzeneseleninic anhydride at low temperature ; transforming the hydroxy compound of formula Int-32 into the lactone Int-33 by the following sequence : (1) Reacting compound of formula Int-32 with 2 eq. oftriflic anhydride and 5 eq. of DMSO. (2) followed by reaction with 8 eq. of diisopropylethylamine. (3) followed by reaction with 4 eq of t-butyl alcohol (4) followed by reaction with 7 eq of 2-tert-Butyl-1, 1, 3, 3, tetramethylguanidine (5) followed by reaction with 10 eq of acetic anhydride ; transforming the lactone compound Int-33 into hydroxyl compound Int-34 by removal of MOM protecting group with TMSI ; cleaving the N-trichloroethoxycarbonyl group of the compound of formula Int-34 into compound Int-35 by reaction with Zn/AcOH ; transforming the amino compound Int-35 into the corresponding a-keto lactone compound Int-36 by reaction with N-methyl pyridinium carboxaldehyde chloride followed by DBU ; The conversion of the Intermediate compound Int-25 into ET-743 using cysteine derivative Int-37 can be made in a similar manner and with the same reagents than with cysteine derivative Int-29 with the exception of transformations (f) and (g). The reaction sequence is exemplified in the following Scheme VII : It will readily be appreciated that these synthetic routes can readily be modified, particularly by appropriate change of the starting material and reagents, so as to provide compounds of this invention with different fused ring systems or different substituents.

NOVEL ACTIVE COMPOUNDS We have found that compounds of the invention have activity in the treatment of cancers, such as leukaemias, lung cancer, colon cancer, kidney cancer and melanoma.

Thus, the present invention provides a method of treating any mammal, notably a human, affected by cancer which comprises administering to the affected individual a therapeutically effective amount of a compound of the invention, or a pharmaceutical composition thereof.

The present invention also relates to pharmaceutical preparations, which contain as active ingredient a compound or compounds of the invention, as well as the processes for their preparation.

Examples of pharmaceutical compositions include any solid (tablets, pills, capsules, granules, etc.) or liquid (solutions, suspensions or emulsions) with suitable composition or oral, topical or parenteral administration, and they may contain the pure compound or in combination with any carrier or other pharmacologically active compounds. These compositions may need to be sterile when administered parenterally.

Administration of the compounds or compositions of the present invention may be by any suitable method, such as intravenous infusion, oral preparations, intraperitoneal and intravenous administration. We prefer that infusion times of up to 24 hours are used, more preferably 2-12 hours, with 2-6 hours most preferred. Short infusion times which allow treatment to be carried out without an overnight stay in hospital are especially desirable. However, infusion may be 12 to 24 hours or even longer if required. Infusion may be carried out at suitable intervals of say 2 to 4 weeks. Pharmaceutical compositions containing compounds of the invention may be delivered by liposome or nanosphere encapsulation, in sustained release formulations or by other standard delivery means.

The correct dosage of the compounds will vary according to the particular formulation, the mode of application, and the particular situs, host and tumour being treated. Other factors like age, body weight, sex, diet, time of administration, rate of excretion, condition of the host, drug combinations, reaction sensitivities and severity of the disease shall be taken into account. Administration can be carried out continuously or periodically within the maximum tolerated dose.

The compounds and compositions of this invention may be used with other drugs to provide a combination therapy. The other drugs may form part of the same composition, or be provided as a separate composition for administration at the same time or a different time. The identity of the other drug is not particularly limited, and suitable candidates include : a) drugs with antimitotic effects, especially those which target cytoskeletal elements, including microtubule modulators such as taxane drugs (such as taxol, paclitaxel, taxotere, docetaxel), podophylotoxins or vinca alkaloids (vincristine, vinblastine) ; b) antimetabolite drugs such as 5-fluorouracil, cytarabine, gemcitabine, purine analogues such as pentostatin, methotrexate) ; c) alkylating agents such as nitrogen mustards (such as cyclophosphamide or ifosphamide) ; d) drugs which target DNA such as the antracycline drugs adriamycin, doxorubicin, pharmorubicin or epirubicin ; e) drugs which target topoisomerases such as etoposide ; f) hormones and hormone agonists or antagonists such as estrogens, antiestrogens (tamoxifen and related compounds) and androgens, flutamide, leuprorelin, goserelin, cyprotrone or octreotide ; g) drugs which target signal transduction in tumour cells including antibody derivatives such as herceptin ; h) alkylating drugs such as platinum drugs (cis-platin, carbonplatin, oxaliplatin, paraplatin) or nitrosoureas ; i) drugs potentially affecting metastasis of tumours such as matrix metalloproteinase inhibitors ; j) gene therapy and antisense agents ; k) antibody therapeutics ; 1) other bioactive compounds of marine origin, notably the didemnins such as aplidine ; m) steroid analogues, in particular dexamethasone ; n) anti-inflammatory drugs, in particular dexamethasone ; and o) anti-emetic drugs, in particular dexamethasone.

The present invention also extends to the compounds of the invention for use in a method of treatment, and to the use of the compounds in the preparation of a composition for treatment of cancer.

CYTOTOXIC ACTIVITY Cell Cultures. Cells were maintained in logarithmic phase of growth in Eagle's Minimum Essential Medium, with Earle's Balanced Salts, with 2. 0 mM L-glutamine, with non-essential amino acids, without sodium bicarbonate (EMEM/neaa) ; supplemented with 10% Fetal Calf Serum (FCS), 10-2 M sodium bicarbonate and 0. 1 g/1 penicillin-G + streptomycin sulfate.

A simple screening procedure has been carried out to determine and compare the antitumour activity of these compounds, using an adapted form of the method described by Bergeron et al (1984). The tumour cell line employed have been P-388 (suspension culture of a lymphoid neoplasm from DBA/2 mouse), A-549 (monolayer culture of a human lung carcinoma), HT-29 (monolayer culture of a human colon carcinoma) and MEL-28 (monolayer culture of a human melanoma).

P-388 cell were seeded into 16 mm wells at 1x104 cells per well in 1 ml aliquots of MEM 5FCS containing the indicated concentration of drug. A separate set of cultures without drug was seeded as control growth to ensure that cells remained in exponential phase of growth. All determinations were carried out in duplicate. After three days of incubation at 37°C, 10% C02 in a 98% humid atmosphere, an approximately IC50 was determined by comparing the growth in wells with drug to the growth in wells control.

A-549, HT-29 and MEL-28 were seeded into 16 mm wells at 2x104 cells per well in 1 ml aliquots of MEM 10FCS containing the indicated concentration of drug. A separate set of cultures without drug was seeded as control growth to ensure that cells remained in exponential phase of growth. All determinations were carried out in duplicate. After three days of incubation at 37°C, 10% C02 in a 98% humid atmosphere, the wells were stained with 0. 1% Crystal Violet. An approximately ICSO was determined by comparing the growth in wells with drug to the growth in wells control.

1. Raymond J. Bergeron, Paul F. Cavanaugh, Jr., Steven J. Kline. Robert G.

Hughes, Jr., Gary T. Elliot and Carl W. Porter. Antineoplastic and antiherpetic activity of spermidine catecholamide iron chelators. Biochem. Bioph. Res. Comm. 1984, 121 (3), 848-854.

2. Alan C. Schroeder, Robert G. Hughes, Jr. and Alexander Bloch. Effects of Acyclic Pyrimidine Nucleoside Analoges. J. Med. Chem. 1981, 24 1078-1083.

Examples of biological activities of the compounds described in the present application are in Table IV (IC50 (ng/mL)) on the following pages. Compound X1 X2 X3 R1 P-388 A-549 HT-29 MEL-28 DU-145 4a AcNH- Me OH Ac 0.1 0,.5 0.1 0.5 0.1 4b F3CCONH- Me OH Ac 0.5 0.5 0.5 0.5 0.5 4c CH3(CH2)2CONH- Me OH Ac 0.1 0.1 0.1 0.1 0.1 4d(CH3)2CHCH2CONH- Me OH Ac 0.5 0.5 0.5 0.5 0.5 4e CH3(CH2)6CONH- Me OH Ac 1.0 1.0 1.0 1.0 1.0 4f CH3(CH2)14CONH- Me OH Ac 100 100 100 10 100 4g PhCONH- Me OH Ac 0.1 0.5 0.5 0.5 0.5 4h CinnCONH- Me OH Ac 0.5 0.5 0.5 0.5 0.5 4i p-F3C-CinnCONH- Me OH Ac 1.0 1.0 1.0 1.0 1.0 4k BiotinCONH- Me OH Ac 10 10 10 10 10 41 HO2CCH2CH2CONH- Me OH Ac 100 100 100 100 100 4n BnNH- Me OH Ac 0.5 0.5 0.5 0.5 0.5 4o PrNH- Me OH Ac 1.0 1.0 4p NH2-ValCONH- Me OH Ac 0.5 0.5 0.5 0.5 0.5 4q Ac-N-ValCONH- Me OH Ac 1.0 1.0 1.0 1.0 1.0 4r CinnCO-N-ValCONH- Me OH Ac 0.5 0.5 0.5 0.5 0.5 4s NH2-Ala-ValCONH- Me OH Ac 1.0 1.0 1.0 1.0 4t Ac-N-Ala-ValCONH- Me OH Ac 100 100 10 10 10 4u CinnCO-N-Ala-ValCONH- Me OH Ac 5.0 5.0 5.0 5.0 5.0 4v NH2-AlaCONNH- Me OH Ac 1.0 1.0 1.0 1.0 1.0 4x CinnCO-N-AlaCONH- Me OH Ac 1.0 1.0 1.0 1.0 1.0 4y FmSCH2CH(NHAlloc)CONH- Me OH Ac 50 50 50 50 50 19 HO- Me OH Ac 1.0 1.0 1.0 1.0 1.0 21a AcO- Me OH Ac 0.5 1.0 1.0 1.0 1.0 21c CH3(CH2)2COO- Me OH Ac 1.0 1.0 1.0 1.0 1.0 21e CH3(CH2)6COO- Me OH Ac 1.0 1.0 1.0 1.0 1.0 21f CH3(CH2)14COO- Me OH Ac >1000 >1000 21h CinnCOO Me OH Ac 1.0 1.0 1.0 1.0 1.0 211l MeSO3- Me OH Ac 1.0 1.0 1.0 1.0 1.0 22a* *AcO- Me OH Ac 1.0 1.0 27 NH2 Me OH Ac 5.0 5.0 5.0 5.0 - 23 NH2 Me CN H 10 10 24 NH2 H CN Ac 100 100 3a AcNH- Me CN Ac 0.5 0.5 0.5 0.5 0.5 25 AcNH- Me CN H 10 10 26 AcNH- H CN Ac 100 100 3b F3CCONH- Me CN Ac 1.0 1.0 1.0 1.0 1.0 3c CH3(CH2)2CONH- Me CN Ac 0.1 0.1 0.1 0.1 0.1 3d(CH2)2CHCH2CONH- Me CN Ac 0.1 0.1 0.1 0.1 0.1 3e CH3(CH2)6 CONH- Me CN Ac 1.0 1.0 1.0 1.0 1.0 3f CH3(CH2)14 CONH- Me CN Ac >1#103 >1#103 >1#103 >1#103 >1#103 3g PhCONH- Me CN Ac 0.1 0.1 0.1 0.1 0.1 3h CinnCONH- Me CN Ac 0.5 0.5 0.5 0.5 0.5 3i p-F3C-CinnCONH- Me CN Ac 5.0 5.0 5.0 5.0 3j PhtN- Me CN Ac 5 5 5 5 5 6 2-MeO2C-C6H4-CONH- Me CN Ac 1 1 1 1 1 3k BiotinNH- Me CN Ac 10 10 5 5 5 31 HO2C(CH2)2CONH- Me CN Ac 100 100 100 100 100 3m (CH3)2N- Me CN Ac 10 10 10 10 10 3n BnNH- Me CN Ac 0.5 0.5 0.5 0.5 0.5 3o PrNH- Me CN Ac 5 5 3p NH2-ValCONH- Me CN Ac 1 1 1 1 1 3q Ac-N-ValCONH- Me CN Ac 1 1 1 1 1 3r CinnCO-NValCONH- Me CN Ac 1 1 1 1 1 3s NH2-Ala-ValCONH- Me CN Ac 1 1 1 1 1 3t Ac-N-Ala-ValCONH- Me CN Ac 10 10 10 10 10 3u CinnCO-N-Ala-ValCONH- Me CN Ac 5 5 1 1 1 3v NH2-AlaCONH- Me CN Ac 1 1 1 1 1 3wAc-N-alaCONH- Me CN Ac 1 1 1 1 1 3x CinnCO-N-AlaCONH- Me CN Ac 0.1 0.1 0.1 0.1 0.1 3y FmSCH2CH(NHAlloc)CONH- Me CN Ac 10 10 10 50 50 3z FmSCH2CH(NH2)CONH- Me CN Ac 50 50 50 50 50 28 Cl3CCH2OCONH- Me CN Ac 1.0 1.0 1.0 1.0 1.0 15 HO-Me CN Ac 5 5 5 5 5 16* *HO Me CN Ac 10 10 17a AcO- Me CN Ac .1 0.1 0.1 0.1 0.1 17b F3CCOO- Me CN Ac 5.0 5.0 5.0 5.0 5.0 17c CH3(CH2)2COO- Me CN Ac 1.0 1.0 1.0 1.0 1.0 17e CH3(CH2)6COO- Me CN Ac 1.0 1.0 1.0 1.0 1.0 17f CH3(CH2)14COO- Me CN Ac >1000 >1000 >1000 >1000 >1000 17h CinnCOO- Me CN Ac 0.1 0.1 0.1 0.1 0.1 171l MeSO3- Me CN Ac 1 1 1 1 - - 18a* *AcO- Me CN Ac 1.0 1.0 1.0 1.0 1.0 EXAMPLES Example 1 Method A : To a solution of 1 equiv. of 1 (23 for 25) coevaporated with anhydrous toluene in CH2CI2 (0. 08M) under Argon were added 1. 2 equiv. of the anhydride. The reaction was followed by TLC and quenched with acid or base, extracted with CH CI, and the organic layers dried with Na2SO4. Flash chromatography gives pure compounds.

Compound 2a (using Ac20 as the anhydride) :'H NMR (300 MHz, CDCl3) : b 6. 77 (s, 1H), 6. 04 (dd, 2H), 5. 53 (bd, 1H), 5. 18 (dd, 2H), 5. 02 (d, 1H), 4. 58 (ddd, 1H), 4. 52 (bs, 1H), 4. 35 (d, 1H), 4. 27 (s, 1H), 4. 19-4. 15 (m, 2H), 3. 75 (s, 3H), 3. 55 (s, 3H), 3. 54-3. 43 (m, 2H), 2. 93 (bd, 2H), 2. 35-2. 02 (m, 2H), 2. 28 (s, 3H), 2. 27 (s, 3H), 2. 18 (s, 3H), 2. 02 (s, 3H), 1. 89 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 170. 5, 168. 7, 168. 4, 149. 7, 148. 5, 145. 8, 141. 0, 140. 4, 131. 0, 130. 5, 125. 7, 124. 5, 120. 3, 117. 9, 113. 5, 113. 4, 102. 0, 99. 1, 61. 4, 60. 3, 59. 6, 58. 8, 55. 0, 54. 5, 52. 1, 41. 8, 41. 3, 32. 6, 23. 7, 20. 9, 20. 2, 16. 1, 9. 5 ; ESI-MS m/z : Calcd. for C35H4oN40, oS : 708. 2.

Found (M+H+) : 709. 2.

Compound 2b (using (F3CCO) 20 as the anhydride) :'H NMR (300 MHz, CDCl3) : 8 6. 74 (s, 1H), 6. 41 (bd, 1H), 6. 05 (dd, 2H), 5. 17 (dd, 2H), 5. 05 (d, 1H), 4. 60 (bp, 1H), 4. 54-4. 51 (m, 1H), 4. 36-4. 32 (m, 2H), 4. 25-4. 19 (m, 2H), 3. 72 (s, 3H), 3. 56 (s, 3H), 3. 48-3. 43 (m, 2H), 2. 99-2. 82 (m, 2H), 2. 46-2. 41 (m, 1H), 2. 30-2. 03 (m, 1H), 2. 29 (s, 3H), 2. 24 (s, 3H), 2. 17 (s, 3H), 2. 04 (s, 3H) ; l3C NMR (75 MHz, CDC13) : 8 168. 9, 168. 5, 156. 3, 155. 8, 155. 3, 149. 3, 148. 5, 146. 0, 141. 2, 140. 6, 132. 0, 130. 2, 124. 8, 120. 2, 117. 9, 113. 2, 102. 1, 99. 2, 61. 5, 60. 6, 59. 7, 59. 1, 58. 7, 57. 5, 54. 9, 54. 6, 52. 9, 42. 0, 41. 4, 31. 6, 23. 8, 20. 2, 14. 1, 9. 6 ; ESI-MS m/z : Calcd. for C35H37F3N4O, oS : 762. 2. Found (M+H+) : 763. 2.

Compound 21 (using succinic anhydride) :'H NMR (300 MHz, CDC13) : 8 6. 79 (s, 1H), 6. 04 (dd, 2H), 5. 63 (bd, 1H), 5. 18 (dd, 2H), 5. 02 (d, 1H), 4. 59-4. 53 (m, 2H), 4. 35 (d, 1H), 4. 28 (s, 1H), 4. 21-4. 17 (m, 2H), 3. 76 (s, 3H), 3. 57 (s, 3H), 3. 54-3. 44 (m, 2H), 2. 92 (bd, 2H), 2. 69-2. 63 (m, 2H), 2. 53-2. 48 (m, 2H), 2. 38-2. 07 (m, 2H), 2. 28 (s, 6H), 2. 18 (s, 3H), 2. 02 (s, 3H) ; ESI-MS m/z : Calcd. for C37H42N4Ol2S : 766. 2. Found (M+H+) : 767. 3.

Compound 25 (from Compound 23 using 1 equiv of AczO as the anhydride) :'H NMR (300 MHz, CDC13) : 8 6. 59 (s, 1H), 5. 97 (dd, 2H), 5. 87 (s, 1H), 5. 53 (s, 1H), 5. 51 (d, 1H), 5. 00 (d, 1H), 4. 62-4. 58 (m, 1H), 4. 44 (s 1H), 4. 31 (s, 1H), 4. 29 (d, 1H), 4. 16 (d, 1H), 4. 09 (dd, 1H), 3. 79 (s, 3H), 3. 54-3. 52 (m, 1H), 3. 44-3. 42 (m, IH), 2. 93-2. 91 (m, 2H), 2. 46 (dd, 1H), 2. 33 (s, 3H), 2. 23 (dd, 1H), 2. 15 (s, 3H), 2. 14 (s, 3H), 1. 90 (s, 1H) ;"C NMR (75 MHz, CDC13) : 5 170. 1, 169. 0, 148. 3, 146. 4, 146. 0, 143. 0, 136. 4, 130. 7, 129. 2, 120. 4, 119. 0, 118. 1, 112. 4, 112. 3, 107. 8, 101. 4, 61. 1, 60. 5, 59. 2, 58. 8, 54. 7, 54. 5, 51. 6, 43. 3, 41. 4, 31. 4, 23. 8, 22. 9, 16. 2, 8. 7 ; ESI-MS m/z : Calcd. for C3lH34N408S : 580. 2. Found (M+H+) : 581. 3.

Example 2 Method B : To a solution of 1 equiv. of 1 (2p for 2t and 9, and 11 for 13e-f) and 1. 5 equiv. of acid coevaporated twice with anhydrous toluene in CH2C12 (0. 05M) under Argon, were added 2 equiv. of DMAP and 2 equiv. of EDC-HCI. The reaction was stirred for 3h 30 min. After this time was diluted with CH2CI2, washed with brine and the organic layer dried with Na2SO4. Flash chromatography gives pure compounds.

Compound 2e (using CH3 (CH2) 6CO2H as the acid) :'H NMR (300 MHz, CDC13) : 8 6. 76 (s, 1H), 6. 04 (dd, 2H), 5. 50 (bd, 1H), 5. 18 (dd, 2H), 5. 02 (d, 1H), 4. 60 (ddd, 1H), 4. 53 (bp, 1H), 4. 35 (d, 1H), 4. 28 (s, 1H), 4. 19 (d, 1H), 4. 18 (dd, 1H), 3. 76 (s, 3H), 3. 58 (s, 3H), 3. 48-3. 43 (m, 2H), 2. 93 (bd, 2H), 2. 29-1. 99 (m, 4H), 2. 29 (s, 3H), 2. 28 (s, 3H), 2. 17 (s, 3H), 2. 03 (s, 3H), 1. 31-1. 23 (m, 10H), 0. 89 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 5 171. 9, 170. 6, 168. 4, 149. 6, 148. 5, 145. 8, 141. 0, 140. 4, 130. 9, 130. 5, 125. 7, 124. 5, 120. 4, 117. 9, 113. 4, 102. 0, 99. 2, 61. 5, 60. 2, 59. 6, 59. 3, 58. 7, 57. 5, 55. 0, 54. 5, 51. 9, 41. 8, 41. 4, 36. 4, 32. 7, 31. 7, 29. 3, 29. 1, 25. 4, 23. 7, 22. 6, 20. 3, 16. 1, 14. 0, 9. 6 ; ESI-MS m/z : Calcd. for C4lH52N4OIoS : 792. 3. Found (M+H+) : 793. 3.

Compound 2f (using CH3 (CH2), 4CO2H as the acid) : IH NMR (300 MHz, CDC13) : b 6. 76 (s, 1H), 6. 05 (dd, 2H), 5. 50 (bd, 1H), 5. 18 (dd, 2H), 5. 02 (d, 1H), 4. 60 (ddd, 1H), 4. 56-4. 50 (bp, 1H), 4. 35 (d, 1H), 4. 28 (bs, 1H), 4. 20 (d, 1H), 4. 18 (dd, 1H), 3. 76 (s, 3H), 3. 57 (s, 3H), 3. 54-3. 44 (m, 2H), 2. 93-2. 92 (bd, 2H), 2. 37-2. 01 (m, 4H), 2. 29 (s, 3H), 2. 28 (s, 3H), 2. 18 (s, 3H), 2. 03 (s, 3H), 1. 60-1. 56 (m, 2H), 1. 40-1. 20 (m, 24H), 0. 88 (t, 3H) ; ESI-MS m/z : Calcd. for C49H6sN4Olos : 904. 5.

Found (M+H+) : 905. 5.

Compound 2g (using PhC02H as the acid) :'H NMR (300 MHz, CDCI3) : 5 7. 69-7. 66 (m, 2H), 7. 57-7. 46 (m, 3H), 6. 69 (s, 1H), 6. 35 (d, 1H), 6. 06 (dd, 2H), 5. 14 (dd, 2H), 5. 07 (d, 1H), 4. 76 (dt, 1H), 4. 58 (bp, 1H), 4. 36-4. 33 (m, 2H), 4. 24- 4. 18 (m, 2H), 3. 62 (s, 3H), 3. 55 (s, 3H), 3. 49-3. 46 (m, 2H), 2. 94 (bd, 2H), 2. 62-2. 55 (m, 1H), 2. 28-1. 93 (m, 1H), 2. 28 (s, 3H), 2. 16 (s, 3H), 2. 04 (s, 3H), 1. 93 (s, 3H) ;'3C NMR (75 MHz, CDC13) : 8 170. 5, 168. 4, 166. 4, 149. 3, 148. 4, 145. 9, 141. 1, 140. 6, 134. 5, 134. 2, 131. 6, 131. 4, 130. 5, 128. 6, 126. 9, 125. 2, 124. 5, 120. 7, 118. 0, 113. 4, 102. 0, 99. 2, 61. 6, 60. 2, 59. 8, 59. 2, 58. 6, 57. 4, 55. 0, 54. 6, 53. 2, 41. 9, 41. 4, 32. 9, 23. 9, 20. 2, 15. 7, 9. 6 ; ESI-MS m/z : Calcd. for C4oH42N40ioS : 770. 3. Found (M+H) : 771. 3.

Compound 2k (using (+)-biotin as the acid) :'H NMR (300 MHz, CDCI3) : 8 6. 78 (s, 1H), 6. 04 (dd, 2H), 6. 00 (s, 1H), 5. 80 (s, 1H), 5. 39 (bd, 1H), 5. 18 (dd, 3H), 4. 78 (d, 1H), 4. 64-4. 51 (m, 3H), 4. 34-4. 28 (m, 3H), 4. 19 (dd, 1H), 3. 77 (s, 3H), 3. 57 (s, 3H), 3. 47- 3. 39 (m, 2H), 3. 19-3. 13 (m, 1H), 3. 02-2. 74 (m, 4H), 2. 28-1. 47 (m, 10H), 2. 28 (s, 6H), 2. 14 (s, 3H), 2. 02 (s, 3H) ; 3C NMR (75 MHz, CDC13) : 5 172. 3, 171. 3, 165. 6, 163. 7, 149. 6, 148. 4, 145. 9, 141. 0, 140. 5, 131. 1, 130. 7, 125. 8, 124. 8, 120. 2, 118. 4, 113. 7, 113. 3, 102. 0, 99. 1, 61. 5, 61. 4, 61. 3, 60. 0, 59. 6, 59. 3, 58. 4, 57. 4, 56. 1, 55. 2, 54. 6, 51. 8, 42. 2, 41. 3, 41. 1, 35. 2, 32. 1, 28. 2, 28. 1, 25. 4, 24. 0, 20. 3, 16. 1, 9. 5 ; ESI- MS m/z : Calcd. for C43Hs2N60nS2 : 892. 3. Found (M+H+) : 894. 1.

Compound 2t (from Compound 2p using Ac-L-alanine as the acid) :'H NMR (300 MHz, CDCI3) : 8 6. 74 (s, 1H), 6. 60-6. 56 (m, 1H), 6. 26 (bt, 1H), 6. 04 (dd, 2H), 5. 58 (bt, 1H), 5. 17 (dd, 2H), 5. 00 (d, 1H), 4. 64-4. 60 (m, 1H), 4. 56 (bp, 1H), 4. 48 (dt, 1H), 4. 35 (d, 1H), 4. 29 (s, 1H), 4. 20-4. 14 (m, 2H), 4. 12-4. 05 (m, 1H), 3. 75, 3. 76 (2s, 3H), 3. 56 (s, 3H), 3. 47-3. 42 (m, 2H), 2. 98-2. 89 (m, 2H), 2. 42-1. 98 (m, 3H), 2. 42 (s, 3H), 2. 28 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H), 1. 98 (s, 3H), 1. 36, 1. 33 (2d, 3H), 1. 06, 1. 03 (2d, 3H), 0. 94, 0. 93 (2d, 3H) ;"C NMR (75 MHz, CDC13) : 8 171. 9, 170. 2, 169. 6, 169. 7, 168. 5, 149. 6, 148. 6, 145. 9, 141. 1, 140. 5, 131. 8, 130. 3, 125. 4, 124. 4, 120. 3, 117. 9, 113. 4, 102. 0, 99. 2, 61. 5, 60. 2, 59. 6, 59. 4, 59. 3, 58. 5, 57. 8, 57. 7, 57. 4, 54. 9, 54. 5, 52. 0, 51. 9, 48. 9, 48. 8, 42. 0, 41. 3, 32. 7, 32. 2, 32. 1, 23. 8, 23. 1, 23. 1, 20. 3, 19. 2, 19. 2, 19. 1, 18. 4, 17. 7, 17. 7, 16. 2, 9. 5. ESI-MS m/z : Calcd. for C43H54N60, 2S : 878. 3. Found (M+H+) : 879. 2.

Compound 2w (using Ac-L-alanine as the acid) :'H NMR (300 MHz, CDC13) : 8 6. 89, 6. 77 (2s, 1H), 6. 25 (dd, 1H), 6. 05 (dd, 2H), 5. 72, 5. 55 (2bd, 1H), 5. 22-5. 13 (2dd, 2H), 5. 02, 5. 01 (2d, 1H), 4. 60-4. 18 (m, 7H), 3. 77, 3. 74 (2s, 3H), 3. 56 (s, 3H), 3. 48-3. 43 (m, 2H), 2. 93-2. 91 (bd, 2H), 2. 42-1. 98 (m, 2H), 2. 42, 2. 37 (2s, 3H), 2. 29, 2. 28 (2s, 3H), 2. 17, 2. 15 (2s, 3H), 2. 03 (s, 3H), 1. 99, 1. 97 (2s, 3H), 1. 46, 1. 22 (2d, 3H) ; 13C NMR (75 MHz, CDC13) : 8 171. 5, 170. 1, 169. 9, 169. 3, 169. 2, 168. 6, 149. 8, 149. 4, 148. 7, 148. 5, 145. 9, 141. 1, 140. 5, 140. 4, 132. 0, 131. 6, 130. 6, 130. 2, 125. 5, 124. 9, 124. 4, 120. 4, 120. 2, 117. 9, 113. 6, 113. 4, 102. 0, 99. 2, 61. 6, 61. 5, 60. 4, 60. 3, 59. 6, 59. 5, 59. 4, 59. 2, 58. 8, 58. 3, 57. 5, 55. 0, 55. 0, 54. 6, 52. 2, 51. 8, 48. 6, 48. 5, 42. 1, 42. 0, 41. 4, 32. 5, 32. 4, 23. 8, 23. 7, 23. 2, 23. 2, 20. 3, 19. 9, 19. 8, 16. 0, 15. 9, 9. 6.

ESI-MS m/z : Calcd. for C38H45N5OllS : 779. 3. Found (M+H+) : 780. 2.

Compound 2y (using FmSCH2CH (NHAlloc) CO2H as the acid) :'H NMR (300 MHz, CDC13) : 8 7. 77-7. 67 (m, 4H), 7. 42-7. 26 (m, 4H), 6. 75 (s, 1H), 6. 12 (bd, 1H), 6. 04 (dd, 2H), 5. 97-5. 88 (m, 1H), 5. 53 (bd, 1H), 5. 35-5. 21 (m, 2H), 5. 15 (dd, 2H), 4. 99 (d, 1H), 4. 61- 4. 55 (m, 4H), 4. 34 (d, 1H), 4. 30 (s, 1H), 4. 20-4. 17 (m, 4H), 3. 70 (s, 3H), 3. 54 (s, 3H), 3. 46 (d, 1H), 3. 45-3. 40 (m, 1H), 3. 21-3. 14 (m, 1H), 3. 04-2. 83 (m, SH), 2. 41- 2. 03 (m, 2H), 2. 33 (s, 3H), 2. 23 (s, 3H), 2. 15 (s, 3H), 2. 03 (s, 3H) ; ESI-MS m/z : Calcd. for C54H57N5012S2 : 1031. 3. Found (M+) : 1032. 2.

Compound 7 (using Boc-L-valine as the acid) :'H NMR (300 MHz, CDCI3) : 5 6. 80 (s, 1H), 6. 04 (dd, 2H), 5. 86 (bd, 1H), 5. 15 (dd, 2H), 5. 02 (d, 1H), 4. 98 (bd, 1H), 4. 63-4. 60 (m, 1H), 4. 55 (bp, 1H), 4. 35 (d, 1H), 4. 30 (s, 1H), 4. 22-4. 16 (m, 2H), 3. 83 (dd, 1H), 3. 76 (s, 3H), 3. 56 (s, 3H), 3. 48-3. 42 (m, 2H), 2. 93-2. 90 (m, 2H), 2. 41-2. 03 (m, 3H), 2. 41 (s, 3H), 2. 28 (s, 3H), 2. 15 (s, 3H), 2. 03 (s, 3H), 1. 46 (s, 9H), 1. 01 (d, 3H), 0. 87 (d, 3H) ; 3C NMR (75 MHz, CDC13) : 5 170. 4, 170. 2, 168. 5, 165. 2, 155. 3, 148. 6, 145. 9, 141. 1, 140. 5, 131. 6, 130. 4, 125. 5, 124. 5, 120. 5, 118. 0, 113. 5, 113. 4, 102. 0, 99. 2, 61. 6, 60. 0, 59. 6, 59. 3, 58. 4, 57. 5, 55. 0, 54. 6, 52. 1, 42. 0, 41. 4, 32. 7, 31. 6, 28. 3, 23. 8, 20. 2, 19. 1, 17. 5, 16. 3, 9. 6. ESI-MS m/z : Calcd. for C43H55N50 2S : 865. 4. Found (M+H+) : 866. 3.

Compound 8 (using Boc-L-alanine as the acid) :'H NMR (300 MHz, CDC) : 8 6. 81 (s, 1H), 6. 04 (dd, 2H), 5. 86 (bp, 1H), 5. 16 (dd, 2H), 5. 03 (bp, 1H), 5. 02 (d, 1H), 4. 56-4. 50 (m, 2H), 4. 34 (d, 1H), 4. 29 (s, 1H), 4. 20-4. 15 (m, 2H), 3. 98-3. 78 (m, 1H), 3. 75 (s, 3H), 3. 55 (s, 3H), 3. 47-3. 43 (m, 2H), 2. 91 (bd, 2H), 2. 37-2. 02 (m, 2H), 2. 37 (s, 3H), 2. 27 (s, 3H), 2. 15 (s, 3H), 2. 02 (s, 3H), 1. 46 (s, 9H), 1. 37 (d, 3H) ;'3C NMR (75 MHz, CDC13) : 8 171. 5, 170. 1, 168. 4, 154. 6, 149. 5, 148. 5, 145. 8, 141. 0, 140. 4, 131. 3, 130. 4, 125. 6, 124. 4, 120. 3, 117. 9, 113. 3, 101. 9, 99. 1, 61. 4, 60. 1, 59. 6, 59. 2, 58. 5, 57. 4, 54. 9, 54. 5, 52. 1, 49. 9, 41. 8, 41. 3, 32. 4, 28. 3, 23. 8, 20. 2, 19. 5, 16. 1, 9, 5. ESI-MS m/z : Calcd. for C4lHsiNsOl2S : 837. 3. Found (M+H+) : 838. 4.

Compound 9 (using Boc-L-alanine as the acid) :'H NMR (300 MHz, CDC13) : 8 6. 76 (s, 1H), 6. 66 (bd, 1H), 6. 04 (dd, 2H), 5. 58 (bd, 1H), 5. 17 (dd, 2H), 5. 01 (d, 1H), 4. 99 (bp, 1H), 4. 66-4. 63 (m, 1H), 4. 56 (bp, 1H), 4. 35 (d, 1H), 4. 29 (s, 1H), 4. 19-4. 05 (m, 4H), 3. 76 (s, 3H), 3. 56 (s, 3H), 3. 47-3. 42 (m, 2H), 2. 92-2. 89 (m, 2H), 2. 44-2. 02 (m, 3H), 2. 44 (s, 3H), 2. 28 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H), 1. 41 (s, 9H), 1. 32 (d, 3H), 1. 03 (d, 3H), 0. 93 (d, 3H) ; 13C NMR (75 MHz, CDC13) : 5 172. 1, 170. 2, 169. 7, 168. 5, 149. 7, 148. 7, 145. 9, 141. 0, 140. 5, 132. 0, 130. 2, 125. 3, 124. 4, 120. 3, 117. 9, 113. 5, 102. 0, 99. 2, 61. 5, 60. 2, 59. 6, 59. 4, 58. 5, 57. 7, 57. 4, 55. 0, 54. 6, 51. 9, 50. 2, 42. 0, 41. 4, 32. 7, 32. 2, 28. 2, 23. 8, 20. 3, 19. 1, 18. 1, 17. 8, 16. 3, 9. 6. ESI-MS m/z : Calcd. for C46H6oN6013S : 936. 4. Found (M) : 937. 2.

Compound 13e (using 5 equiv. of CH3 (CH2) 6CO2H as the acid, 7 equiv. of DMAP and 7 equiv. of EDC HCI) :'H NMR (300 MHz, CDC13) : 8 6. 68 (s, 1H), 6. 04 (dd, 2H), 5. 17 (dd, 2H), 5. 02-4. 98 (m, 2H), 4. 56 (bp, 1H), 4. 34 (d, 1H), 4. 28 (s, 1H), 4. 19 (d, 1H), 4. 11 (dd, 1H), 3. 78 (s, 3H), 3. 56 (s, 3H), 3. 46 (d, 1H), 3. 42-3. 39 (m, 1H), 2. 89-2. 87 (m, 2H), 2. 32-1. 96 (m, 4H), 2. 30 (s, 3H), 2. 26 (s, 3H), 2. 17 (s, 3H), 2. 03 (s, 3H), 1. 60-1. 55 (m, 2H), 1. 32-1. 23 (m, 8H), 0. 90 (t, 3H) ;'3C NMR (75 MHz, CDC13) : 8 172. 5, 168. 6, 167. 1, 148. 9, 148. 2, 145. 8, 141. 1, 140. 6, 130. 7, 125. 3, 125. 1, 124. 7, 120. 9, 118. 1, 113. 6, 113. 1, 102. 0, 99. 2, 71. 4, 61. 5, 60. 0, 59. 8, 59. 2, 58. 6, 57. 4, 55. 0, 54. 6, 41. 6, 41. 5, 33. 8, 31. 7, 29. 1, 28. 9, 24. 7, 23. 9, 22. 6, 20. 2, 15. 9, 14. 0, 9. 6. ESI-MS m/z : Calcd. for C4lH5lN3O"S : 793. 3. Found (M+H+) : 794. 9.

Compound 13f (using 4 equiv. of CH3 (CH2) l4CO2H as the acid, 6 equiv. of DMAP and 6 equiv. of EDC HCl) : lH NMR (300 MHz, CDC13) : 8 6. 68 (s, 1H), 6. 04 (dd, 2H), 5. 17 (dd, 2H), 5. 02-4. 98 (m, 2H), 4. 56 (bp, 1H), 4. 34 (d, 1H), 4. 28 (s, 1H), 4. 19 (d, 1H), 4. 12 (dd, 1H), 3. 78 (s, 3H), 3. 57 (s, 3H), 3. 46 (d, 1H), 3. 45-3. 41 (m, 1H), 2. 89-2. 87 (m, 2H), 2. 37-1. 96 (m, 4H), 2. 30 (s, 3H), 2. 26 (s, 3H), 2. 17 (s, 3H), 2. 04 (s, 3H), 1. 63-1. 58 (m, 2H), 1. 35-1. 23 (m, 24H), 0. 88 (t, 3H) ; X3C NMR (75 MHz, CDC13) : 8 172. 6, 168. 6, 167. 1, 148. 9, 148. 2, 145. 8, 141. 1, 140. 6, 130. 7, 125. 3, 125. 1, 124. 7, 120. 9, 118. 1, 113. 6, 113. 1, 102. 0, 99. 2, 71. 4, 61. 5, 60. 0, 59. 8, 59. 2, 58. 6, 57. 4, 55. 0, 54. 6, 41. 6, 41. 5, 33. 9, 31. 9, 31. 7, 30. 9, 29. 7, 29. 5, 29. 3, 29. 3, 29. 2, 29. 1, 24. 7, 23. 9, 22. 7, 20. 2, 15. 9, 14. 1, 9. 6.

Example 3 Method C : To a solution of 1 equiv. of 1 coevaporated twice with anhydrous toluene in CH2C12 (0. 05M) under Argon, were added 1. 05 equiv. of phthalic anhydride. After 30 min the reaction was cold to 0°C and 2. 5 equiv. of Et3N and 1. 5 equiv. of CIC02Et were added. 5 min later the reaction was warmed to RT and stirred for 7h. Then it was diluted with CH2Cl2, washed with a saturated solution of NaHC03 and the organic layer dried with Na2S04. Flash chromatography (hex/EtOAc, 3 : 2) gives 2d in 85% yield.

Compound 2j :'H NMR (300 MHz, CDC13) : 8 7. 91-7. 70 (m, 4H), 6. 67 (s, 1H), 6. 06 (dd, 2H), 5. 19 (dd, 2H), 5. 05 (d, 1H), 4. 64-4. 62 (m, 2H), 4. 37 (d, 1H), 4. 32 (s, 1H), 4. 20 (d, 1H), 4. 12 (dd, 1H), 3. 79 (s, 3H), 3. 58 (s, 3H), 3. 50 (d, 1H), 3. 41- 3. 40 (m, 1H), 2. 85-2. 83 (m, 2H), 2. 36-2. 11 (m, 2H), 2. 33 (s, 3H), 2. 31 (s, 3H), 2. 14 (s, 3H), 2. 05 (s, 3H) ; ESI-MS m/z : Calcd. for C4lH40N4OllS : 796. 2. Found (M+H+) : 797. 2.

Example 4 Method D : To a solution of 1 equiv. of 1 in CH3CN/CH2CI2 3 : 1 (0. 025M) under Argon, were added 1 equiv. of formaline solution (37%) and 1 equiv. of NaBH3CN. The solution was stirred at room temperature for 30 min. Then, 2 equiv. of acetic acid were added the solution which turned to orange-yellow was stirred for lh 30 min. After this time the reaction mixture was diluted with CH2Cl2, neutralized with NaHC03 and extracted with CH2Cl2. The organic layer was dried with Na2S04. Flash chromatography gives the pure compound.

Compound 2m :'H NMR (300 MHz, CDC13) : 8 6. 66 (s, 1H), 6. 03 (dd, 2H), 5. 17 (dd, 2H), 4. 98 (d, 1H), 4. 58 (bp, 1H), 4. 32 (d, 1H), 4. 25 (s, 1H), 4. 15-4. 13 (m, 1H), 3. 95 (dd, 1H), 3. 78 (s, 3H), 3. 56 (s, 3H), 3. 54-3. 41 (m, 3H), 2. 92-2. 80 (m, 2H), 2. 33 (s, 3H), 2. 17 (s, 3H), 2. 17-2. 07 (bp, 6H), 2. 16 (s, 3H), 2. 04 (s, 3H), 1. 86 (dd, 2H) ; ESI-MS m/z : Calcd. for C35H42N409S : 694. 3. Found (M+H+) : 695. 3.

Example 5 Method E : To a solution of 1 equiv. of 1 (3p for 3q-r, 3s for 3u, 3v for 3x, 11 for 13c, 13h, 1311 and 24 for 26) in CH2CI2 (0. 08M) under Argon at RT were added 1. 1 equiv. of pyridine. Then the reaction was cold to 0°C and 1. 1 equiv of the acid chloride were added. 5 min later the reaction was warmed to RT and stirred for 45 min. Then it was diluted with CH2C12, washed with a saturated solution of NaCl and the organic layer dried with Na2S04. Flash chromatography gives pure compounds.

Compound 2c (using butyryl chloride) :'H NMR (300 MHz, CDCl3) : 8 6. 76 (s, 1H), 6. 04 (dd, 2H), 5. 52 (bd, 1H), 5. 17 (dd, 2H), 5. 02 (d, 1H), 4. 61 (ddd, 1H), 4. 52 (bp, 1H), 4. 34 (dd, 1H), 4. 27 (s, 1H), 4. 19 (d, 1H), 4. 17 (dd, 1H), 3. 75 (s, 3H), 3. 56 (s, 3H), 3. 47-3. 43 (m, 2H), 2. 92 (bd, 2H), 2. 34-1. 98 (m, 4H), 2. 28 (s, 3H), 2. 27 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H), 1. 71-1. 58 (m, 2H), 0. 96 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 8 171. 7, 170. 6, 168. 4, 149. 6, 148. 5, 145. 8, 141. 0, 140. 4, 131. 0, 130. 5, 125. 7, 124. 6, 120. 4, 117. 9, 113. 4, 102. 0, 99. 1, 61. 5, 60. 1, 59. 6, 59. 2, 58. 6, 57. 4, 55. 0, 54. 5, 51. 9, 41. 8, 41. 3, 38. 2, 32. 7, 23. 7, 20. 2, 18. 8, 16. 1, 13. 7, 9. 5. ESI-MS m/z : Calcd. for C37H44N40loS : 736. 3. Found (M+H+) : 737. 2.

Compound 2d (using isovaleryl chloride) :'H NMR (300 MHz, CDC13) : 8 6. 76 (s, 1H), 6. 05 (dd, 2H), 5. 50 (bd, 1H), 5. 17 (dd, 2H), 5. 02 (d, 1H), 4. 63 (ddd, 1H), 4. 53 (bp, 1H), 4. 35 (dd, 1H), 4. 28 (s, 1H), 4. 20 (d, 1H), 4. 18 (dd, 1H), 3. 76 (s, 3H), 3. 56 (s, 3H), 3. 47-3. 43 (m, 2H), 2. 92 (bd, 2H), 2. 30- 1. 92 (m, SH), 2. 30 (s, 3H), 2. 28 (s, 3H), 2. 17 (s, 3H), 2. 03 (s, 3H), 0. 99 (d, 3H), 0. 93 (d, 3H) ; 13C NMR (75 MHz, CDC13) : 8 171. 3, 170. 6, 168. 4, 149. 6, 148. 5, 141. 0, 140. 5, 130. 9, 130. 5, 125. 7, 124. 6, 120. 4, 118. 0, 113. 5, 113. 4, 102. 0, 99. 2, 61. 5, 60. 1, 59. 6, 59. 3, 58. 6, 57. 5, 55. 0, 54. 6, 51. 8, 45. 6, 41. 9, 41. 4, 31. 8, 25. 8, 23. 8, 22. 5, 22. 4, 20. 2, 16. 3, 9. 6. ESI- MS m/z : Calcd. for C38H46N4OloS : 750. 3. Found (M+H+) : 751. 3.

Compound 2h (using cinnamoyl chloride) :'H NMR (300 MHz, CDC13) : 8 7. 61 (d, 1H), 7. 55-7. 51 (m, 2H), 7. 44-7. 37 (m, 3H), 6. 85 (s, 1H), 6. 24 (d, 1H), 6. 05 (dd, 2H), 5. 72 (d, 1H), 5. 16 (dd, 2H), 5. 05 (d, 1H), 4. 71 (ddd, 1H), 4. 54 (bp, 1H), 4. 35 (dd, 1H), 4. 29 (s, 1H), 4. 22-4. 17 (m, 2H), 3. 68 (s, 3H), 3. 56 (s, 3H), 3. 48-3. 44 (m, 2H), 2. 97-2. 95 (m, 2H), 2. 51- 2. 45 (m, 1H), 2. 27-2. 03 (m, 1H), 2. 27 (s, 6H), 2. 19 (s, 3H), 2. 03 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 170. 5, 168. 4, 164. 5, 149. 7, 148. 5, 145. 8, 142. 1, 141. 0, 140. 4, 134. 7, 131. 1, 130. 5, 129. 8, 128. 8, 127. 9, 125. 5, 124. 4, 120. 4, 119. 7, 118. 0, 113. 4, 113. 3, 102. 0, 99. 1, 61. 4, 60. 3, 59. 6, 59. 2, 58. 8, 57. 4, 54. 9, 54. 5, 52. 6, 41. 7, 41. 4, 32. 7, 23. 8, 20. 2, 16. 3, 9. 6.

ESI-MS m/z : Calcd. for C42H44N4OIpS : 796. 3. Found (M+H+) : 797. 2.

Compound 2i (using trans-3- (trifluoromethyl)-cinnamoyl chloride) :'H NMR (300 MHz, CDC13) : 8 7. 82-7. 51 (m, 5H), 6. 85 (s, 1H), 6. 29 (d, 1H), 6. 05 (dd, 2H), 5. 75 (d, 1H), 5. 17 (dd, 2H), 5. 05 (d, 1H), 4. 73-4. 69 (m, 1H), 4. 55 (bp, 1H), 4. 36 (d, 1H), 4. 39 (s, 1H), 4. 23-4. 18 (m, 2H), 3. 69 (s, 3H), 3. 57 (s, 3H), 3. 48-3. 44 (m, 2H), 2. 96 (bd, 2H), 2. 49-2. 44 (m, 1H), 2. 27-2. 04 (m, 1H), 2. 27 (s, 6H), 2. 19 (s, 3H), 2. 04 (s, 3H) ;'3C NMR (75 MHz, CDCl3) : 8 170. 3, 168. 4, 163. 8, 149. 7, 148. 5, 145. 9, 141. 1, 140. 5, 135. 5, 134. 6, 131. 6, 131. 0, 130. 6, 129. 5, 126. 3, 126. 2, 125. 6, 124. 4, 123. 7, 123. 6, 121. 5, 120. 3, 117. 9, 113. 5, 113. 3, 102. 0, 99. 2, 61. 4, 60. 4, 59. 6, 59. 2, 58. 9, 57. 5, 54. 9, 54. 5, 52. 6, 41. 8, 41. 4, 32. 6, 23. 8, 20. 3, 16. 2, 9. 6. ESI-MS m/z : Calcd. for C43H43N4F30lOoS : 864. 3. Found (M+H+) : 865. 0.

Compound 3q (from Compound 3p using acetyl chloride) : IH NMR (300 MHz, CDC13) : 6 6. 54 (s, 1H), 6. 08 (d, 1H), 6. 05 (dd, 2H), 5. 81 (s, 1H), 5. 59 (d, 1H), 5. 02 (d, 1H), 4. 67 (dt, 1H), 4. 58 (bp, 1H), 4. 29 (s, 1H), 4. 26 (dd, 1H), 4. 21-4. 16 (m, 1H), 4. 09 (dd, 1H), 3. 80 (s, 3H), 3. 45-3. 42 (m, 2H), 2. 91-2. 88 (m, 2H), 2. 49 (s, 3H), 2. 29-1. 98 (m, 3H), 2. 29 (s, 3H), 2. 16 (s, 3H), 2. 03 (s, 3H), 1. 98 (s, 3H), 1. 06 (d, 3H), 0. 96 (d, 3H) ; 13C NMR (75 MHz, CDC13) : 8 170. 2, 169. 5, 168. 6, 148. 1, 145. 9, 143. 3, 141. 1, 140. 4, 130. 4, 130. 1, 120. 4, 120. 2, 118. 5, 118. 0, 113. 5, 102. 0, 61. 4, 60. 4, 59. 3, 58. 8, 57. 7, 54. 7, 54. 6, 51. 8, 42. 0, 41. 5, 32. 7, 32. 3, 23. 8, 23. 3, 20. 5, 19. 1, 18. 0, 16. 2, 9. 6. ESI-MS m/z : Calcd. for C38H45N5OIoS : 763. 3. Found (M+H+) : 764. 3 Compound 3r (from Compound 3p using cinnamoyl chloride) :'H NMR (300 MHz, CDC13) : 8 7. 59 (d, 1H), 7. 50-7. 46 (m, 2H), 7. 37-7. 34 (m, 3H), 6. 57 (s, 1H), 6. 42 (d, 1H), 6. 30 (d, 1H), 6. 05 (dd, 2H), 5. 81 (s, 1H), 5. 64 (d, 1H), 5. 03 (d, 1H), 4. 70-4. 67 (m, 1H), 4. 58 (bp, 1H), 4. 30-4. 24 (m, 3H), 4. 21-4. 17 (m, 2H), 3. 82 (s, 3H), 3. 45 (bd, 2H), 2. 92-2. 89 (m, 2H), 2. 56 (s, 3H), 2. 28-2. 03 (m, 3H), 2. 28 (s, 3H), 2. 17 (s, 3H), 2. 03 (s, 3H), 1. 10 (d, 3H), 1. 00 (d, 3H) ; 13C NMR (75 MHz, CDC13) : 8 170. 2, 170. 1, 169. 4, 168. 5, 165. 3, 148. 1, 145. 9, 143. 4, 141. 2, 140. 4, 134. 8, 130. 5, 130. 1, 129. 7, 128. 8, 127. 8, 120. 6, 120. 4, 120. 2, 118. 5, 118. 0, 113. 5, 113. 5, 102. 0, 61. 4, 60. 4, 59. 4, 58. 9, 57. 7, 54. 7, 54. 6, 51. 9, 42. 0, 41. 5, 32. 7, 23. 8, 20. 5, 19. 2, 18. 0, 16. 4, 9. 6. ESI-MS m/z : Calcd. for C45H49N50IOsS : 851. 3. Found (M+H+) : 852. 3 Compound 3u (from Compound 3s using cinnamoyl chloride) : IH NMR (300 MHz, CDC) : 8 7. 63 (d, 1H), 7. 50-7. 47 (m, 2H), 7. 38-7. 35 (m, 3H), 6. 62 (d, 1H), 6. 55 (s, 1H), 6. 41 (d, 1H), 6. 35 (d, 1H), 6. 05 (dd, 2H), 5. 82 (s, 1H), 5. 60 (d, 1H), 5. 02 (d, 1H), 4. 68-4. 60 (m, 2H), 4. 58 (bp, 1H), 4. 29 (s, 1H), 4. 26 (dd, 1H), 4. 21-4. 15 (m, 2H), 4. 10 (dd, 1H), 3. 79 (s, 3H), 3. 45-3. 43 (m, 2H), 2. 91-2. 88 (m, 2H), 2. 48 (s, 3H), 2. 30-2. 03 (m, 3H), 2. 28 (s, 3H), 2. 16 (s, 3H), 2. 03 (s, 3H), 1. 41 (d, 3H), 1. 04 (d, 3H), 0. 94 (d, 3H) ;"C NMR (75 MHz, CDC13) : 8 171. 8, 170. 2, 169. 6, 168. 5, 165. 4, 148. 0, 145. 9, 143. 3, 141. 6, 141. 1, 140. 5, 134. 7, 130. 6, 129. 8, 129. 8, 128. 8, 127. 8, 120. 3, 120. 1, 118. 7, 118. 0, 113. 5, 102. 0, 61. 5, 60. 3, 59. 4, 58. 8, 57. 8, 54. 7, 54. 6, 51. 9, 49. 0, 42. 1, 41. 5, 32. 6, 32. 3, 23. 8, 20. 5, 19. 2, 18. 6, 17. 7, 16. 3, 9. 6. ESI-MS m/z : Calcd. for C48H54N6011S : 922. 4. Found (M+H+) : 923. 1.

Compound 3x (from Compound 3v using cinnamoyl chloride) :'H NMR (300 MHz, CDC13) : 8 7. 60 (d, 1H), 7. 49-7. 46 (m, 2H), 7. 37-7. 34 (m, 3H), 6. 59 (s, 1H), 6. 48 (d, 1H), 6. 39 (d, 1H), 6. 05 (dd, 2H), 5. 84 (s, 1H), 5. 58 (d, 1H), 5. 03 (d, 1H), 4. 64-4. 59 (m, 1H), 4. 58 (bp, 1H), 4. 36-4. 8 (m, 1H), 4. 28 (s, 1H), 4. 26 (d, 1H), 4. 22-4. 17 (m, 2H), 3. 81 (s, 3H), 3. 45-3. 43 (m, 2H), 2. 92 (d, 2H), 2. 53 (s, 3H), 2. 28-2. 03 (m, 2H), 2. 28 (s, 3H), 2. 16 (s, 3H), 2. 03 (s, 3H), 1. 54 (d, 3H) ;) ; 13C NMR (75 MHz, CDC13) : 8 171. 4, 170. 1, 168. 6, 164. 9, 148. 2, 145. 9, 143. 2, 141. 1, 134. 8, 130. 5, 130. 0, 129. 7, 128. 8, 127. 8, 120. 4, 120. 4, 120. 0, 118. 8, 118. 0, 113. 6, 113. 4, 102. 0, 61. 4, 60. 6, 60. 4, 59. 3, 59. 1, 54. 8, 54. 6, 51. 7, 48. 7, 41. 9, 41. 5, 32. 5, 23. 8, 20. 5, 20. 0, 16. 2, 9. 6. ESI- MS m/z : Calcd. for C43H45N501OS : 823. 3. Found (M+H+) : 824. 3.

Compound 13c (from Compound 11 using 20 equiv. of butyryl chloride and 30 equiv. of pyr) :'H NMR (300 MHz, CDC13) : 8 6. 68 (s, 1H), 6. 04 (dd, 2H), 5. 17 (dd, 2H), 5. 02 (bt, 1H), 5. 01 (d, 1H), 4. 57 (bp, 1H), 4. 34 (dd, 1H), 4. 29 (s, 1H), 4. 19 (d, 1H), 4. 12 (dd, 1H), 3. 78 (s, 3H), 3. 56 (s, 3H), 3. 46 (d, 1H), 3. 45-3. 42 (m, 1H), 2. 88 (bd, 2H), 2. 30-2. 16 (m, 3H), 2. 30 (s, 3H), 2. 26 (s, 3H), 2. 16 (s, 3H), 2. 03 (s, 3H), 2. 02-1. 96 (m, 1H), 1. 68-1. 56 (m, 2H), 0. 98 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 8 172. 5, 168. 8, 167. 3, 149. 1, 148. 4, 146. 0, 141. 3, 140. 9, 131. 0, 125. 6, 125. 0, 121. 2, 118. 3, 113. 8, 113. 3, 102. 2, 99. 4, 71. 7, 61. 7, 60. 3, 60. 0, 59. 4, 58. 8, 57. 6, 55. 2, 54. 9, 41. 9, 41. 7, 36. 1, 32. 0, 24. 2, 20. 5, 18. 5, 16. 1, 13. 9, 9. 8.

ESI-MS m/z : Calcd. for C37H43N3OllS : 737. 3. Found (M+Na+) : 760. 2.

Compound 13h (from Compound 11 using 5 equiv. of cinnamoyl chloride, 7. 5 equiv. of pyr and CH3CN as cosolvent) :'H NMR (300 MHz, CDC13) : 8 7. 68 (d, 1H), 7. 56- 7. 53 (m, 2H), 7. 43-7. 39 (m, 3H), 6. 72 (s, 1H), 6. 30 (d, 1H), 6. 05 (dd, 2H), 5. 22-5. 13 (m, 3H), 5. 04 (d, 1H), 4. 58 (bp, 1H), 4. 35 (d, 1H), 4. 31 (s, 1H), 4. 21 (d, 1H), 4. 15 (dd, 1H), 3. 79 (s, 3H), 3. 57 (s, 3H), 3. 48 (d, 1H), 3. 43-3. 39 (m, 1H), 2. 90-2. 88 (m, 2H), 2. 47-2. 41 (m, 1H), 2. 31 (s, 3H), 2. 24 (s, 3H), 2. 17 (s, 3H), 2. 07-2. 03 (m, 1H), 2. 04 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 168. 6, 167. 1, 165. 6, 148. 8, 148. 2, 145. 7, 141. 1, 140. 6, 134. 4, 130. 9, 130. 7, 130. 4, 128. 9, 128. 2, 128. 1, 125. 2, 124. 7, 120. 9, 118. 1, 117. 3, 113. 7, 113. 1, 102. 0, 99. 2, 71. 9, 61. 5, 60. 0, 59. 8, 59. 3, 58. 5, 57. 4, 54. 9, 54. 6, 41. 7, 41. 5, 31. 8, 23. 9, 20. 2, 16. 0, 9. 6.

ESI-MS m/z : Calcd. for C42H43N30nS : 797. 3. Found (M+H+) : 798. 8.

Compound 1311 (from Compound 11 using 5 equiv. of methanesulfonyl chloride and 5 equiv. of Et3N as base) :'H NMR (300 MHz, CDC13) : 8 6. 65 (s, 1H), 6. 04 (dd, 2H), 5. 17 (dd, 2H), 5. 00 (d, 1H), 4. 93 (dd, 1H), 4. 58 (bp, 1H), 4. 34 (dd, 1H), 4. 29 (s, 1H), 4. 16-4. 12 (m, 2H), 3. 77 (s, 3H), 3. 56 (s, 3H), 3. 46 (d, 1H), 3. 44-3. 39 (m, 1H), 3. 11 (s, 3H), 2. 96-2. 81 (m, 2H), 2. 50-2. 42 (m, 1H), 2. 30 (s, 3H), 2. 26 (s, 3H), 2. 18 (s, 3H), 2. 04-1. 97 (m, 1H), 2. 03 (s, 3H) ; ESI-MS m/z : Calcd. for C34H39N3012S2 : 745. 2.

Found (M+H+) : 746. 2.

Compound 26 (from Compound 24 using 1. 05 equiv of acetyl chloride and without base) :'H NMR (300 MHz, CDC13) : 8 6. 51 (s, 1H), 6. 05 (d, 2H), 5. 95 (s, 1H), 5. 60 (d, 1H), 5. 59 (bp, 1H), 5. 03 (d, 1H), 4. 58-4. 53 (m, 2H), 4. 27 (s, 1H), 4. 26 (d, 1H), 4. 20- 4. 16 (m, 2H), 3. 43-3. 42 (m, 2H), 2. 90-2. 88 (m, 2H), 2. 27-2. 11 (m, 2H), 2. 27 (s, 3H), 2. 24 (s, 3H), 2. 14 (s, 3H), 2. 03 (s, 3H), 1. 85 (s, 3H) ;"C NMR (75 MHz, CDC13) : 8 170. 4, 169. 5, 168. 9, 145. 8, 144. 5, 140. 9, 140. 4, 139. 9, 127. 1, 123. 6, 120. 1, 119. 8, 119. 2, 118. 1, 113. 5, 113. 4, 102. 0, 61. 3, 60. 4, 59. 2, 58. 9, 54. 7, 54. 5, 52. 0, 41. 7, 41. 4, 32. 3, 23. 5, 22. 8, 20. 6, 16. 2, 9. 6 ; ESI-MS m/z : Calcd. for C32H34N409S : 650. 2. Found (M+H+) : 651. 3.

Example 6 Method F : To a solution of 1 equiv. of 1 in DMF (0. 03M) under Argon at room temperature, were added 0. 9 equiv. of Cs2CO3 and 0. 9 equiv on BnBr. After 2h 30 min the reaction was quenched with IgL of AcOH, diluted with Hex/EtOAc (1 : 3), washed with H20 and extracted with Hex/EtOAc (1 : 3). The organic layer was dried with Na2SO4. Flash chromatography give pure compound 2n.

Compound 2n :'H NMR (300 MHz, CDC13) : 8 7. 32-7. 20 (m, 5H), 6. 56 (s, 1H), 6. 02 (dd, 2H), 5. 15 (dd, 2H), 5. 04 (d, 1H), 4. 51 (bp, 1H), 4. 32 (d, 1H), 4. 25-4. 23 (m, 2H), 4. 12 (dd, 1H), 3. 74 (s, 3H), 3. 62 (dd, 2H), 3. 56 (s, 3H), 3. 44-3. 40 (m, 2H), 3. 38-3. 20 (m, 1H), 3. 19-2. 84 (m, 2H), 2. 36-1. 91 (m, 2H), 2. 29 (s, 3H), 2. 19 (s, 3H), 2. 03 (s, 3H), 1. 91 (s, 3H) ;'3C NMR (75 MHz, CDC13) : 8 172. 7, 168. 6, 149. 3, 148. 2, 145. 6, 140. 9, 140. 4, 139. 9, 131. 5, 130. 3, 128. 3, 128. 1, 126. 9, 124. 9, 124. 7, 120. 9, 118. 1, 113. 8, 113. 2, 101. 9, 99. 1, 61. 5, 59. 7, 59. 6, 59. 5, 59. 2, 58. 9, 57. 4, 54. 9, 54. 7, 51. 3, 41. 5, 41. 4, 33. 3, 23. 8, 20. 3, 15. 3, 9. 6. ESI-MS m/z : Calcd. for C4oH44N409S : 756. 3. Found (M+Na+) : 779. 2.

Example 7 Method G : To a solution of 1 equiv. of 2a-n, 2t, 2w, 2y, 11, 12*, 13a-c, 13e-f, 13h, 1311, 14a* or 7-9 in CH3CN/CH2C12 5 : 4 (0. 026M) under Argon were added 6 equiv. of NaI and 6 equiv. of fresh distilled TMSC1. After 20 min the reaction was quenched with a saturated solution of Na2S204, diluted with CH2C12, washed with Na2S204 (x3), or with NaCl. The aqueous layer extracted with CH2C12. The organic layer was dried with Na2SO4. Flash chromatography gives pure compounds 3a-n, 3p, 3s-t, 3v-w, 3y-z, 15, 16*, 17a-c, 17e-f, 17h, 1711, 18a*.

Compound 3a (from 2a) :'H NMR (300 MHz, CDC13) : 5 6. 56 (s, 1H), 6. 04 (dd, 2H), 5. 78 (s, 1H), 5. 52 (bd, 1H), 5. 02 (d, 1H), 4. 58 (ddd, 1H), 4. 53 (bs, 1H), 4. 27-4. 25 (m, 2H), 4. 19- 4. 15 (m, 2H), 3. 77 (s, 3H), 3. 44-3. 43 (m, 2H), 2. 92-2. 90 (m, 2H), 2. 36-2. 02 (m, 2H), 2. 36 (s, 3H), 2. 30 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H), 1. 88 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 170. 5, 168. 8, 168. 4, 148. 1, 145. 8, 143. 1, 141. 0, 140. 3, 130. 7, 129. 9, 129. 0, 120. 3, 119. 0, 117. 9, 113. 5, 102. 0, 61. 3, 60. 3, 60. 2, 59. 3, 58. 9, 54. 7, 54. 5, 51. 9, 41. 8, 41. 4, 32. 4, 23. 7, 22. 8, 20. 4, 16. 0, 9. 5 ; ESI-MS m/z : Calcd. for C33H36N409S : 664. 2. Found (M+H+) : 665. 2.

Compound 3b (from 2b) :'H NMR (300 MHz, CDC13) : 8 6. 52 (s, 1H), 6. 41 (bd, 1H), 6. 05 (dd, 2H), 5. 72 (s, 1H), 5. 05 (d, 1H), 4. 60 (bp, 1H), 4. 54-4. 51 (m, 1H), 4. 32 (s, 1H), 4. 26-4. 18 (m, 3H), 3. 74 (s, 3H), 3. 46-3. 42 (m, 2H), 2. 97-2. 80 (m, 2H), 2. 44-2. 38 (m, 1H), 2. 30-2. 03 (m, 1H), 2. 30 (s, 3H), 2. 27 (s, 3H), 2. 15 (s, 3H), 2. 03 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 168. 8, 168. 5, 156. 3, 155. 8, 155. 3, 147. 6, 146. 0, 143. 1, 141. 2, 140. 5, 130. 5, 129. 9, 120. 7, 120. 6, 120. 1, 118. 0, 117. 9, 113. 2, 101. 1, 61. 4, 60. 7, 60. 1, 59. 5, 58. 9, 54. 6, 54. 5, 52. 8, 42. 0, 41. 5, 31. 9, 23. 8, 20. 4, 15. 6, 9. 6 ; ESI-MS m/z : Calcd. for C33H33F3N409S : 718. 2. Found (M+H+) : 719. 2.

Compound 3c (from 2c) :'H NMR (300 MHz, CDC13) : 5 6. 54 (s, 1H), 6. 03 (dd, 2H), 5. 82 (s, 1H), 5. 49 (bd, 1H), 5. 02 (d, 1H), 4. 61 (ddd, 1H), 4. 53 (bp, 1H), 4. 27-4. 24 (m, 2H), 4. 19- 4. 15 (m, 2H), 3. 76 (s, 3H), 3. 44-3. 41 (m, 2H), 2. 90 (bd, 2H), 2. 31-1. 94 (m, 4H), 2. 31 (s, 3H), 2. 28 (s, 3H), 2. 15 (s, 3H), 2. 02 (s, 3H), 1. 67-1. 57 (m, 2H), 0. 95 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 8 171. 8, 170. 5, 148. 0, 145. 8, 143. 1, 141. 0, 140. 4, 130. 8, 129. 0, 120. 4, 120. 2, 119. 0, 118. 0, 113. 4, 102. 0, 61. 4, 60. 2, 59. 4, 58. 9, 54. 7, 54. 5, 51. 7, 41. 8, 41. 4, 38. 2, 32. 6, 23. 8, 20. 5, 18. 8, 16. 0, 13. 7, 9. 6. ESI-MS m/z : Calcd. for C35H4oN409S : 692. 2. Found (M+H+) : 693. 9.

Compound 3d (from 2d) : 1H NMR (300 MHz, CDC13) : 8 6. 54 (s, 1H), 6. 04 (dd, 2H), 5. 76 (s, 1H), 5. 48 (bd, 1H), 5. 02 (d, 1H), 4. 66-4. 60 (m, 1H), 4. 53 (bp, 1H), 4. 27-4. 23 (m, 2H), 4. 19-4. 15 (m, 2H), 3. 76 (s, 3H), 3. 44-3. 42 (m, 2H), 2. 90 (bd, 2H), 2. 33-1. 90 (m, 5H), 2. 33 (s, 3H), 2. 28 (s, 3H), 2. 15 (s, 3H), 2. 02 (s, 3H), 0. 98 (d, 3H), 0. 92 (d, 3H) ; 13C NMR (75 MHz, CDC13) : 8 171. 3, 170. 6, 168. 5, 148. 0, 145. 8, 143. 1, 141. 1, 140. 4, 130. 8, 129. 0, 127. 6, 120. 5, 120. 3, 119. 1, 118. 0, 113. 5, 102. 0, 74. 2, 61. 4, 60. 3, 59. 4, 58. 8, 54. 7, 54. 6, 51. 7, 45. 5, 41. 9, 41. 5, 32. 7, 25. 8, 23. 8, 22. 5, 22. 4, 20. 5, 16. 2, 9. 6. ESI-MS m/z : Calcd. for C36H42N409S : 706. 3. Found (M+Na') : 729. 2.

Compound 3e (from 2e) :'H NMR (300 MHz, CDC13) : 8 6. 54 (s, 1H), 6. 04 (dd, 2H), 5. 75 (s, 1H), 5. 48 (bd, 1H), 5. 02 (d, 1H), 4. 60 (ddd, 1H), 4. 53 (bp, 1H), 4. 27-4. 24 (m, 2H), 4. 19- 4. 15 (m, 2H), 3. 77 (s, 3H), 3. 48-3. 42 (m, 2H), 2. 91 (bd, 2H), 2. 32-1. 97 (m, 4H), 2. 32 (s, 3H), 2. 28 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H), 1. 62-1. 41 (m, 2H), 1. 390-1. 25 (m, 8H), 0. 89 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 8 172. 0, 170. 6, 168. 4, 148. 0, 145. 8, 143. 1, 141. 0, 140. 4, 130. 8, 129. 0, 120. 4, 120. 2, 119. 0, 118. 0, 113. 7, 113. 5, 102. 0, 61. 4, 60. 3, 59. 4, 58. 9, 54. 7, 54. 6, 51. 8, 41. 8, 41. 5, 36. 3, 32. 6, 31. 7, 29. 3, 29. 1, 25. 4, 23. 8, 22. 6, 20. 5, 16. 1, 14. 0, 9. 6 ; ESI-MS m/z : Calcd. for C39H48N409S : 748. 3. Found (M+H+) : 749. 3.

Compound 3f (from 2f) :'H NMR (300 MHz, CDC13) : 8 6. 55 (s, 1H), 6. 04 (dd, 2H), 5. 73 (s, 1H), 5. 48 (bd, 1H), 5. 02 (d, 1H), 4. 60 (ddd, 1H), 4. 56-4. 50 (bp, 1H), 4. 28-4. 24 (m, 2H), 4. 20-4. 14 (m, 2H), 3. 77 (s, 3H), 3. 44-3. 40 (m, 2H), 2. 92-2. 90 (bd, 2H), 2. 35-1. 95 (m, 4H), 2. 32 (s, 3H), 2. 29 (s, 3H), 2. 16 (s, 3H), 2. 03 (s, 3H), 1. 62-1. 58 (m, 2H), 1. 38-1. 20 (m, 24H), 0. 88 (t, 3H) ; ESI-MS m/z : Calcd. for C47H64N409S : 860. 4.

Found (M+H+) : 861. 5.

Compound 3g (from 2g) : lH NMR (300 MHz, CDC13) : 8 7. 69-7. 66 (m, 2H), 7. 57-7. 45 (m, 3H), 6. 48 (s, 1H), 6. 35 (d, 1H), 6. 06 (dd, 2H), 5. 70 (s, 1H), 5. 07 (d, 1H), 4. 78-4. 74 (m, 1H), 4. 58 (bp, 1H), 4. 33 (s, 1H), 4. 26-4. 18 (m, 3H), 3. 61 (s, 3H), 3. 47-3. 45 (m, 2H), 2. 92 (bd, 2H), 2. 60-2. 53 (m, 1H), 2. 28-1. 93 (m, 1H), 2. 28 (s, 3H), 2. 14 (s, 3H), 2. 04 (s, 3H), 1. 93 (s, 3H) ; 3C NMR (75 MHz, CDC13) : 8 171. 7, 170. 5, 166. 4, 147. 7, 145. 9, 143. 0, 141. 1, 140. 5, 134. 2, 131. 6, 130. 8, 129. 4, 128. 6, 127. 0, 120. 4, 118. 5, 118. 0, 113. 7, 113. 4, 102. 0, 61. 5, 60. 3, 60. 1, 59. 7, 58. 8, 54. 7, 53. 1, 41. 9, 41. 5, 32. 8, 23. 9, 20. 4, 15. 6, 9. 6 ; ESI-MS m/z : Calcd. for C38H38N4O9S : 726. 2. Found (M+H+) : 727. 2.

Compound 3h (from 2h) :'H NMR (300 MHz, CDC13) : 8 7. 60 (d, 1H), 7. 54-7. 51 (m, 2H), 7. 44-7. 38 (m, 3H), 6. 63 (s, 1H), 6. 22 (d, 1H), 6. 05 (dd, 2H), 5. 79 (s, 1H), 5. 73 (d, 1H), 5. 05 (d, 1H), 4. 71 (ddd, 1H), 4. 55 (bp, 1H), 4. 29 (s, 1H), 4. 26 (s, 1H), 4. 21-4. 17 (m, 2H), 3. 68 (s, 3H), 3. 48-3. 42 (m, 2H), 2. 95- 2. 93 (m, 2H), 2. 49-2. 44 (m, 1H), 2. 29-2. 03 (m, 1H), 2. 29 (s, 3H), 2. 27 (s, 3H), 2. 17 (s, 3H), 2. 03 (s, 3H) ;'3C NMR (75 MHz, CDC13) : 8 170. 4, 168. 4, 164. 5, 148. 1, 145. 8, 143. 1, 142. 0, 141. 0, 140. 4, 134. 7, 130. 8, 129. 8, 129. 2, 128. 8, 127. 9, 120. 2, 119. 8, 118. 9, 118. 0, 113. 6, 113. 3, 102. 0, 61. 4, 60. 4, 60. 2, 59. 4, 59. 0, 54. 6, 54. 6, 52. 5, 41. 8, 41. 5, 32. 6, 23. 8, 20. 5, 16. 2, 9. 6. ESI-MS m/z : Calcd. for C4oH4oN409S : 752. 2. Found (M+Na+) : 775. 8. Compound 3i (from 2i) :'H NMR (300 MHz, CDC13) : 8 7. 82 (s, 1H), 7. 66-7. 51 (m, 4H), 6. 64 (s, 1H), 6. 26 (d, 1H), 6. 05 (dd, 2H), 5. 77 (s, 1H), 5. 74 (d, 1H), 5. 05 (d, 1H), 4. 72 (ddd, 1H), 4. 56 (bp, 1H), 4. 29 (s, 1H), 4. 26 (dd, 1H), 4. 22-4. 16 (m, 2H), 3. 70 (s, 3H), 3. 46-3. 44 (m, 2H), 2. 94 (bd, 2H), 2. 47-2. 40 (m, 1H), 2. 30-2. 03 (m, 1H), 2. 30 (s, 3H), 2. 28 (s, 3H), 2. 17 (s, 3H), 2. 03 (s, 3H) ;'3C NMR (75 MHz, CDC13) : 8 170. 3, 163. 9, 148. 1, 143. 1, 141. 1, 140. 4, 135. 6, 131. 7, 130. 9, 129. 5, 129. 0, 126. 2, 123. 6, 121. 7, 120. 3, 118. 0, 113. 3, 102. 0, 99. 2, 61. 4, 60. 5, 60. 2, 59. 4, 59. 1, 54. 7, 54. 6, 52. 5, 41. 8, 41. 5, 32. 6, 23. 8, 20. 5, 16. 2, 9. 6. ESI-MS m/z : Calcd. for C4, H39N4F309S : 820. 2.

Found (M+H+) : 821. 3.

Compound 3j (from 2j) : 1H NMR (300 MHz, CDC13) : 8 7. 77- 7. 68 (m, 4H), 6. 26 (s, 1H), 6. 06 (dd, 2H), 5. 77 (s, 1H), 4. 98 (d, 1H), 4. 61-4. 55 (m, 2H), 4. 33-4. 21 (m, 2H), 4. 09 (d, 1H), 4. 97 (dd, 1H), 3. 97 (s, 3H), 3. 47-3. 31 (m, 2H), 2. 93-2. 77 (m, 2H), 2. 36 (s, 3H), 2. 33-2. 14 (m, 2H), 2. 23 (s, 3H), 2. 17 (s, 3H), 2. 05 (s, 3H) ; ESI-MS m/z : Calcd. for C39H36N4OloS : 752. 2. Found (M+H+) : 753. 2.

Compound 6 :'H NMR (300 MHz, CDC13) : 8 7. 95 (dd, 1H), 7. 66-7. 45 (m, 3H), 6. 13 (s, 1H), 6. 07 (dd, 2H), 5. 88 (d, 1H), 5. 64 (s, 1H), 5. 06 (d, 1H), 4. 83-4. 81 (m, 1H), 4. 53 (bp, 1H), 4. 30-4. 17 (m, 4H), 3. 79 (s, 3H), 3. 61 (s, 3H), 3. 45-3. 40 (m, 2H), 2. 94-2. 85 (m, 2H), 2. 29-2. 04 (m, 2H), 2. 29 (s, 3H), 2. 14 (s, 3H), 2. 04 (s, 6H) ; ESI-MS m/z : Calcd. for C4oH4oN4OnS : 784. 2. Found (M+H+) : 785. 1.

Compound 3k (from 2k) :'H NMR (300 MHz, CDC13) : 8 7. 78 (s, 1H), 6. 55 (s, 1H), 6. 45 (s, 1H), 6. 04 (dd, 2H), 5. 38 (bd, 1H), 5. 29 (bs, 1H), 5. 15 (d, 1H), 4. 66 (m, 1H), 4. 60 (bp, 1H), 4. 55-4. 51 (m, 1H), 4. 40 (d, 1H), 4. 34-4. 29 (m, 2H), 4. 25 (s, 1H), 4. 14 (d, 1H), 3. 79 (s, 3H), 3. 43-3. 39 (m, 2H), 3. 09-3. 05 (m, 1H), 2. 96-2. 90 (m, 3H), 2. 70 (d, 1H), 2. 34-1. 94 (m, 4H), 2. 34 (s, 3H), 2. 30 (s, 3H), 2. 11 (s, 3H), 2. 02 (s, 3H), 1. 81-1. 25 (m, 6H) ;'3C NMR (75 MHz, CDC13) : 5 171. 5, 170. 8, 168. 7, 163. 8, 148. 8, 145. 8, 142. 8, 141. 1, 140. 3, 131. 2, 128. 9, 120. 7, 120. 3, 120. 1, 118. 3, 113. 5, 102. 0, 61. 9, 61. 2, 60. 2, 59. 8, 59. 4, 59. 4, 56. 4, 55. 1, 54. 7, 51. 3, 41. 8, 41. 4, 41. 1, 34. 5, 32. 6, 27. 8, 27. 7, 25. 0, 24. 1, 20. 7, 16. 1, 9. 6 ; ESI-MS m/z : Calcd. for C4lH48N601OS2 : 849. 0. Found (M+H+) : 850. 0.

Compound 31 (from 21) :'H NMR (300 MHz, CDC13) : 8 6. 57 (s, 1H), 6. 04 (dd, 2H), 5. 90 (bp, 1H), 5. 63 (bd, 1H), 5. 02 (d, 1H), 4. 60-4. 55 (m, 2H), 4. 27-4. 17 (m, 4H), 3. 76 (s, 3H), 3. 47- 3. 39 (m, 2H), 2. 90 (bd, 2H), 2. 68-2. 61 (m, 2H), 2. 58-2. 02 (m, 4H), 2. 32 (s, 3H), 2. 29 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 176. 4, 170. 5, 170. 2, 168. 6, 148. 1, 145. 8, 143. 1, 141. 0, 140. 3, 130. 7, 129. 2, 120. 3, 120. 0, 119. 0, 118. 0, 113. 5, 113. 3, 102. 0, 61. 3, 60. 4, 60. 3, 59. 2, 58. 9, 54. 6, 54. 4, 51. 9, 41. 8, 41. 4, 32. 3, 30. 2, 29. 6, 29. 1, 28. 3, 23. 7, 20. 5, 16. 0, 9. 6. ESI-MS m/z : Calcd. for C35H38N4OllS : 722. 2. Found (M+H+) : 723. 2.

Compound 3m (from 2m) :'H NMR (300 MHz, CDC13) : 5 6. 45 (s, 1H), 6. 02 (d, 2H), 5. 67 (s, 1H), 4. 98 (d, 1H), 4. 55 (bp, 1H), 4. 27-4. 22 (m, 2H), 4. 14 (d, 1H), 3. 94 (dd, 1H), 3. 78 (s, 3H), 3. 65-3. 38 (m, 3H), 2. 96-2. 79 (m, 2H), 2. 44-2. 02 (m, 7H), 2. 34 (s, 3H), 2. 20 (s, 3H), 2. 16 (s, 3H), 2. 03 (s, 3H), 1. 88-1. 82 (m, 1H) ; ESI-MS m/z : Calcd. for C33H38N408S : 650. 2. Found (M+H+) : 651. 3.

Compound 3n (from 2n) :'H NMR (300 MHz, CDC13) : 5 7. 31-7. 21 (m, 5H), 6. 37 (s, 1H), 6. 02 (dd, 2H), 5. 67 (s, 1H), 5. 04 (d, 1H), 4. 52 (bp, 1H), 4. 24-4. 22 (m, 3H), 4. 11 (dd, 1H), 3. 73 (s, 3H), 3. 62 (dd, 2H), 3. 42-3. 41 (m, 2H), 3. 19-3. 18 (m, 1H), 3. 03-2. 83 (m, 2H), 2. 34-2. 30 (m, 1H), 2. 30 (s, 3H), 2. 18 (s, 3H), 2. 05-2. 02 (m, 1H), 2. 02 (s, 3H), 1. 93 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 172. 7, 168. 5, 147. 7, 145. 6, 142. 9, 141. 0, 140. 4, 140. 1, 130. 6, 129. 3, 128. 2, 128. 2, 126. 8, 120. 7, 118. 2, 118. 0, 113. 8, 113. 3, 101. 9, 99. 1, 61. 5, 60. 1, 59. 6, 59. 5, 59. 2, 54. 7, 51. 3, 41. 6, 41. 5, 33. 4, 23. 8, 20. 5, 15. 3, 9. 6. ESI-MS m/z : Calcd. for C3gH4oN40gS : 712. 3. Found (M+H+) : 713. 3.

Compound 3p (from 7) :'H NMR (300 MHz, CDC13) : 8 6. 73 (bp. 1H), 6. 51 (s, 1H), 6. 05 (dd, 2H), 5. 03 (d, 1H), 4. 64 (dt, 1H), 4. 55 (bp, 1H), 4. 31 (s, 1H), 4. 26 (dd, 1H), 4. 21 (d, 1H), 4. 17 (dd, 1H), 3. 76 (s, 3H), 3. 49-3. 42 (m, 2H), 2. 99 (d, 1H), 2. 90-2. 88 (m, 2H), 2. 47-1. 97 (m, 3H), 2. 32 (s, 3H), 2. 29 (s, 3H), 2. 13 (s, 3H), 2. 03 (s, 3H), 0. 97 (d, 3H), 0. 79 (d, 3H) ; 13C NMR (75 MHz, CDC13) : 8 173. 6, 170. 4, 168. 5, 147. 6, 145. 9, 143. 1, 141. 1, 140. 5, 130. 8, 129. 0, 120. 8, 120. 6, 118. 8, 118. 0, 113. 5, 113. 3, 102. 0, 61. 5, 60. 6, 60. 2, 60. 0, 59. 6, 58. 6, 54. 7, 54. 6, 51. 9, 42. 0, 41. 5, 33. 0, 31. 6, 23. 9, 20. 4, 19. 6, 16. 8, 16. 2, 9. 6.

ESI-MS m/z : Calcd. for C36H43N509S : 721. 3. Found (M+H+) : 722. 2 Compound 3s (from 9 using 9 equiv of TMSCI and NaI. The reaction was quenched with brine and Na2C03) : IH NMR (300 MHz, CDC13) : 5 7. 74 (d, 1H), 6. 55 (s, 1H), 6. 05 (dd, 2H), 5. 61 (d, 1H), 5. 02 (d, 1H), 4. 68-4. 64 (m, 1H), 4. 57 (bp, 1H), 4. 29 (s, 1H), 4. 27 (dd, 1H), 4. 20-4. 16 (m, 2H), 4. 04 (dd, 1H), 3. 79 (s, 3H), 3. 52-3. 43 (m, 3H), 2. 91-2. 89 (m, 2H), 2. 49 (s, 3H), 2. 29-2. 02 (m, 3H), 2. 29 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H), 1. 33 (d, 3H), 1. 07 (d, 3H), 0. 97 (d, 3H) ; 13C NMR (75 MHz, CDC13) : 8 175. 2, 170. 2, 170. 2, 168. 5, 148. 0, 145. 9, 143. 3, 141. 1, 140. 4, 130. 4, 130. 1, 120. 4, 120. 2, 118. 5, 118. 0, 113. 5, 102. 0, 61. 5, 60. 4, 60. 3, 59. 4, 58. 8, 57. 4, 54. 7, 54. 6, 51. 8, 50. 9, 42. 0, 41. 5, 32. 7, 32. 2, 23. 8, 21. 8, 20. 5, 19. 3, 18. 0, 16. 3, 9. 6. ESI-MS m/z : Calcd. for C39H48N601OS : 792. 3. Found (M+H+) : 793. 3.

Compound 3t (from 2t) : 1H NMR (300 MHz, CDC13) : 8 6. 59 (bd, 1H), 6. 53 (s, 1H), 6. 28-6. 22 (m, 1H), 6. 04 (dd, 2H), 5. 89 (s, 1H), 5. 60, 5. 58 (2d, 1H), 5. 01 (d, 1H), 4. 66- 4. 62 (m, 1H), 4. 57 (bp, 1H), 4. 50-4. 43 (m, 1H), 4. 28 (s, 1H), 4. 25 (d, 1H), 4. 20-4. 12 (m, 2H), 4. 09-4. 04 (m, 1H), 3. 78, 3. 77 (2s, 3H), 3. 47-3. 42 (m, 2H), 2. 90-2. 87 (m, 2H), 2. 46 (s, 3H), 2. 28-1. 98 (m, 3H), 2. 28 (s, 3H), 2. 16, 2. 15 (2s, 3H), 2. 03, 2. 02 (2s, 3H), 1. 98 (s, 3H), 1. 36, 1. 32 (2d, 3H), 1. 05, 1. 03 (2d, 3H), 0. 93 (d, 3H) ;'13C NMR (75 MHz, CDC13) : 8 171. 9, 170. 1, 169. 7, 169. 6, 168. 5, 148. 0, 145. 9, 143. 2, 141. 1, 140. 4, 130. 6, 129. 8, 120. 3, 120. 2, 118. 7, 118. 0, 113. 4, 102. 0, 61. 4, 60. 3, 60. 3, 59. 4, 58. 8, 57. 7, 57. 6, 54. 6, 54. 5, 51. 9, 48. 9, 48. 9, 42. 0, 41. 5, 32. 6, 32. 3, 32. 2, 23. 8, 23. 1, 20. 5, 19. 2, 19. 1, 19. 1, 18. 5, 17. 7, 17. 7, 16. 2, 9. 6. ESI-MS m/z : Calcd. for C41HsoN6OllS : 834. 3. Found (M+H+) : 835. 3.

Compound 3v (from 8 ; the reaction was quenched with brine and Na2C03) :'H NMR (300 MHz, CDC13) : 8 6. 70 (bp, 1H), 6. 52 (s, 1H), 6. 04 (dd, 2H), 5. 03 (d, 1H), 4. 58-4. 53 (m, 2H), 4. 30 (s, 1H), 4. 25 (dd, 1H), 4. 20-4. 14 (m, 2H), 3. 76 (s, 3H), 3. 45-3. 42 (m, 2H), 3. 30 (dd, 1H), 2. 90-2. 88 (m, 2H), 2. 38- 2. 00 (m, 2H), 2. 30 (s, 3H), 2. 29 (s, 3H), 2. 14 (s, 3H), 2. 03 (s, 3H), 1. 25 (d, 3H) 13C NMR (75 MHz, CDC13) : 5 175. 0, 170. 3, 168. 4, 147. 6, 145. 9, 143. 1, 141. 1, 140. 5, 130. 8, 129. 0, 120. 9, 120. 5, 118. 7, 118. 0, 113. 5, 113. 3, 102. 0, 61. 5, 60. 2, 60. 1, 59. 6, 58. 8, 54. 8, 54. 6, 52. 1, 50. 8, 41. 9, 41. 5, 32. 7, 23. 9, 21. 6, 20. 4, 16. 1, 9. 6. ESI-MS m/z : Calcd. for C34H39N509S : 693. 2.

Found (M+H+) : 694. 3.

Compound 3w (from 2w ; the reaction was quenched with brine) :'H NMR (300 MHz, CDC13) : 8 6. 67, 6. 55 (2s, 1H), 6. 30 (m, 1H), 6. 05 (dd, 2H), 5. 86, 5. 79 (2s, 1H), 5. 65, 5. 54 (2bd, 1H), 5. 03, 5. 02 (2d, 1H), 4. 60-4. 17 (m, 7H), 3. 79, 3. 76 (2s, 3H), 3. 45-3. 40 (m, 2H), 2. 92-2. 85 (bd, 2H), 2. 46-1. 95 (m, 2H), 2. 46, 2. 40 (2s, 3H), 2. 29, 2. 28 (2s, 3H), 2. 17, 2. 15 (2s, 3H), 2. 02 (s, 3H), 1. 98, 1. 95 (2s, 3H), 1. 45, 1. 20 (2d, 3H) ;'3C NMR (75 MHz, CDC13) : 8 171. 5, 170. 1, 169. 9, 169. 1, 168. 6, 148. 2, 147. 7, 145. 9, 143. 2, 141. 1, 140. 4, 130. 9, 130. 4, 130. 0, 129. 8, 120. 8, 120. 3, 118. 8, 118. 0, 113. 6, 113. 4, 102. 0, 61. 5, 61. 4, 60. 5, 60. 4, 59. 3, 59. 1, 58. 7, 54. 8, 54. 6, 51. 9, 51. 7, 48. 5, 42. 1, 41. 9, 41. 5, 32. 4, 32. 3, 23. 8, 23. 2, 20. 5, 19. 9, 16. 0, 15. 8, 9. 6. ESI- MS m/z : Calcd. for C36H4lN501OS : 735. 3. Found (M+H+) : 736. 2.

Compound 3y (from 2y) :'H NMR (300 MHz, CDC13) : 8 7. 77-7. 68 (m, 4H), 7. 42-7. 26 (m, 4H), 6. 53 (s, 1H), 6. 05 (bd, 1H), 6. 04 (dd, 2H), 5. 96-5. 87 (m, 1H), 5. 74 (s, 1H), 5. 58 (bd, 1H), 5. 38-5. 20 (m, 2H), 5. 00 (d, 1H), 4. 60-4. 55 (m, 4H), 4. 33-4. 08 (m, 6H), 3. 73 (s, 3H), 3. 44-3. 42 (m, 2H), 3. 19-3. 13 (m, 1H), 3. 05-2. 83 (m, 5H), 2. 38-2. 02 (m, 2H), 2. 38 (s, 3H), 2. 24 (s, 3H), 2. 13 (s, 3H), 2. 03 (s, 3H) ; ESI-MS m/z : Calcd. for Cs2Hs3NsOIlS2 : 987. 3. Found (M+H+) : 988. 1.

Compound 3z was also obtained in the reaction of 2y :'H NMR (300 MHz, CDC13) : 8 7. 76 (d, 2H), 7. 66 (dd, 2H), 7. 42- 7. 30 (m, 4H), 6. 49 (s, 1H), 6. 05 (dd, 2H), 5. 67 (bp, 1H), 5. 02 (d, 1H), 4. 59-4. 54 (m, 2H), 4. 30 (bs, 1H), 4. 25-4. 23 (dd, 1H), 4. 19-4. 09 (m, 3H), 3. 71 (s, 3H), 3. 68-3. 43 (m, 2H), 3. 33 (dd, 1H), 3. 14-2. 85 (m, 5H), 2. 46 (dd, 1H), 2. 35-2. 24 (m, 2H), 2. 25 (s, 3H), 2. 24 (s, 3H), 2. 12 (s, 3H), 2. 03 (s, 3H) ; ESI-MS m/z : Calcd. for C48H49N509S2 : 903. 3. Found (M+H+) : 904. 2.

Compound 15 (from 11) : IH NMR (300 MHz, CDC13) : 5 6. 56 (s, 1H), 6. 03 (dd, 2H), 5. 74 (s, 1H), 5. 04 (d, 2H), 4. 54 (bp, 1H), 4. 26-4. 23 (m, 2H), 4. 20-4. 14 (m, 2H), 4. 02-3. 96 (m, 1H), 3. 78 (s, 3H), 3. 42-3. 39 (m, 2H), 2. 93-2. 90 (m, 2H), 2. 31- 2. 03 (m, 2H), 2. 31 (s, 3H), 2. 29 (s, 3H), 2. 20 (s, 3H), 2. 03 (s, 3H) ; ESI-MS m/z : Calcd. for C31H33N309S : 623. 2. Found (M+H') : 624. 2.

Compound 16* (from 12*) :'H NMR (300 MHz, 45°C, CDC13) : 8 6. 49 (s, 1H), 6. 04 (dd, 2H), 5. 67 (s, 1H), 4. 94 (bd, 1H), 4. 47 (s, 1H), 4. 24-4. 17 (m, 3H), 4. 05 (d, 1H), 3. 80 (s, 3H), 3. 57-3. 55 (m, 2H), 3. 40-3. 37 (m, 1H), 2. 98-2. 90 (m, 1H), 2. 73 (d, 1H), 2. 51-2. 47 (bm, 1H), 2. 33 (s, 3H), 2. 30 (s, 3H), 2. 15 (s, 3H), 2. 02 (s, 3H), 1. 66 (dd, 1H) ; ESI-MS m/z : Calcd. for C31H33N309S : 623. 2. Found (M+H) : 624. 3.

Compound 17a (from 13a) :'H NMR (300 MHz, CDC13) : 8 6. 50 (s, 1H), 6. 04 (dd, 2H), 5. 67 (s, 1H), 5. 02-4. 99 (m, 2H), 4. 56 (bp, 1H), 4. 27 (s, 1H), 4. 25 (dd, 1H), 4. 17 (d, 1H), 4. 11 (dd, 1H), 3. 79 (s, 3H), 3. 44-3. 41 (m, 2H), 2. 88-2. 86 (m, 2H), 2. 31-1. 97 (m, 2H), 2. 31 (s, 3H), 2. 28 (s, 3H), 2. 16 (s, 3H), 2. 03 (s, 3H), 1. 97 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 169. 7, 168. 5, 167. 0, 147. 2, 145. 7, 142. 9, 141. 1, 140. 6, 130. 9, 128. 7, 121. 2, 120. 7, 118. 1, 118. 0, 113. 5, 102. 0, 71. 6, 61. 4, 60. 2, 60. 0, 59. 9, 59. 0, 54. 7, 54. 6, 41. 6, 41. 5, 31. 5, 23. 9, 20. 5, 20. 3, 15. 8, 9. 6. ESI-MS m/z : Calcd. for C33H3sN3OIoS : 665. 2. Found (M+H+) : 666. 1.

Compound 17b (from 13b) :'H NMR (300 MHz, CDC13) : 8 6. 46 (s, 1H), 6. 05 (dd, 2H), 5. 68 (s, 1H), 5. 09 (bt, 1H), 5. 02 (d, 1H), 4. 62 (bp, 1H), 4. 31 (s, 1H), 4. 24 (dd, 1H), 4. 19-4. 14 (m, 2H), 3. 77 (s, 3H), 3. 46-3. 40 (m, 2H), 2. 93-2. 75 (m, 2H), 2. 44-2. 37 (dd, 1H), 2. 32 (s, 3H), 2. 26 (s, 3H), 2. 16 (s, 3H), 2. 10-2. 04 (m, 1H), 2. 04 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8168. 6, 164. 9, 147. 0, 145. 9, 142. 9, 141. 2, 140. 7, 132. 2 (CF3 ?), 130. 6, 129. 5, 125. 1 (CF3 ?), 121. 6, 120. 5 (CF3 ?), 118. 0, 117. 3, 113. 7, 113. 3, 113. 3 (CF3 ?), 102. 1, 74. 8, 61. 4, 60. 6, 60. 1, 59. 9, 58. 9, 54. 6, 41. 7, 41. 6, 31. 0, 23. 9, 20. 4, 15. 5, 9. 6. ESI-MS m/z : Calcd. for C33H32F3N30lOS 719. 2.

Found (M+H+) : 720. 2.

Compound 17c (from 13c) : 1H NMR (300 MHz, CDC13) : 8 6. 47 (s, 1H), 6. 04 (dd, 2H), 5. 66 (s, 1H), 5. 02-4. 99 (m, 2H), 4. 57 (bp, 1H), 4. 28 (s, 1H), 4. 24 (dd, 1H), 4. 18 (d, 1H), 4. 11 (dd, 1H), 3. 79 (s, 3H), 3. 45-3. 41 (m, 2H), 2. 87-2. 85 (m, 2H), 2. 31-1. 99 (m, 4H), 2. 31 (s, 3H), 2. 29 (s, 3H), 2. 15 (s, 3H), 2. 03 (s, 3H), 1. 67-1. 55 (m, 2H), 0. 97 (t, 3H) ; 13C NMR (75 MHz, CDCl3) : 8 172. 3, 168. 5, 167. 0, 147. 2, 145. 8, 142. 9, 141. 1, 140. 6, 131. 0, 128. 8, 121. 2, 120. 8, 118. 1, 118. 1, 113. 6, 113. 1, 102. 0, 71. 4, 61. 4, 60. 2, 59. 9, 59. 9, 58. 8, 54. 8, 54. 7, 41. 6, 35. 9, 31. 7, 24. 0, 20. 4, 18. 2, 15. 8, 13. 7, 9. 6. ESI-MS m/z : Calcd. for C33H39N3OloS : 693. 2. Found (M+H+) : 694. 2.

Compound 17e (from 13e) :'H NMR (300 MHz, CDC13) : 8 6. 47 (s, 1H), 6. 03 (dd, 2H), 5. 66 (s, 1H), 5. 02-4. 98 (m, 2H), 4. 56 (bp, 1H), 4. 27 (s, 1H), 4. 24 (dd, 1H), 4. 17 (d, 1H), 4. 10 (dd, 1H), 3. 79 (s, 3H), 3. 44-3. 42 (m, 2H), 2. 87-2. 85 (m, 2H), 2. 30-1. 98 (m, 4H), 2. 30 (s, 3H), 2. 29 (s, 3H), 2. 15 (s, 3H), 2. 03 (s, 3H), 1. 61-1. 57 (m, 2H), 1. 31-1. 23 (m, 8H), 0. 89 (t, 3H) ; l3C NMR (75 MHz, CDC13) : 8 172. 6, 168. 5, 167. 0, 147. 2, 145. 8, 142. 9, 141. 1, 140. 6, 130. 0, 128. 7, 121. 2, 120. 8, 118. 1, 118. 1, 113. 6, 113. 1, 102. 0, 71. 4, 61. 4, 60. 2, 59. 9, 58. 8, 54. 8, 54. 7, 41. 6, 33. 8, 31. 7, 31. 6, 29. 1, 28. 9, 24. 7, 24. 0, 22. 6, 20. 4, 15. 8, 14. 1, 9. 6. ESI-MS m/z : Calcd. for C39H47N3OoS : 749. 3. Found (M+H+) : 750. 9.

Compound 17f (from 13f) :'H NMR (300 MHz, CDC13) : 8 6. 48 (s, 1H), 6. 04 (dd, 2H), 5. 66 (s, 1H), 5. 02-4. 98 (m, 2H), 4. 57 (bp, 1H), 4. 28 (s, 1H), 4. 25 (dd, 1H), 4. 17 (d, 1H), 4. 10 (dd, 1H), 3. 79 (s, 3H), 3. 44-3. 40 (m, 2H), 2. 87-2. 85 (m, 2H), 2. 37-1. 98 (m, 4H), 2. 31 (s, 3H), 2. 29 (s, 3H), 2. 15 (s, 3H), 2. 03 (s, 3H), 1. 62-1. 55 (m, 2H), 1. 35-1. 26 (m, 24H), 0. 88 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 8 172. 6, 168. 6, 167. 1, 147. 2, 145. 7, 142. 8, 141. 0, 140. 6, 130. 9, 128. 7, 121. 2, 120. 7, 118. 1, 117. 9, 113. 5, 113. 1, 102. 0, 71. 4, 61. 4, 60. 3, 59. 8, 58. 8, 54. 7, 54. 6, 41. 6, 33. 8, 31. 9, 31. 6, 29. 7, 29. 5, 29. 4, 29. 3, 29. 2, 24. 6, 23. 9, 22. 7, 20. 5, 15. 9, 14. 1, 9. 6. ESI-MS m/z : Calcd. for C47H63N3010S : 861. 4. Found (M+H+) : 862. 3.

Compound 17h (from 13h) : I H NMR (300 MHz, CDC13) : 8 7. 64 (d, 1H), 7. 55-7. 52 (m, 2H), 7. 43-7. 40 (m, 3H), 6. 51 (s, 1H), 6. 28 (d, 1H), 6. 05 (dd, 2H), 5. 70 (s, 1H), 5. 17 (bt, 1H), 5. 04 (d, 1H), 4. 58 (bp, 1H), 4. 30 (s, 1H), 4. 26 (d, 1H), 4. 20 (d, 1H), 4. 14 (dd, 1H), 3. 79 (s, 3H), 3. 45 (d, 1H), 3. 42-3. 39 (m, 1H), 2. 92-2. 80 (m, 2H), 2. 42 (dd, 1H), 2. 31 (s, 3H), 2. 26 (s, 3H), 2. 15 (s, 3H), 2. 09-2. 04 (m, 1H), 2. 04 (s, 3H) ; l3C NMR (75 MHz, CDC13) : 8 168. 5, 167. 0, 165. 6, 147. 2, 145. 8, 145. 6, 142. 9, 141. 1, 140. 6, 134. 5, 131. 1, 130. 4, 128. 9, 128. 8, 128. 1, 121. 1, 120. 8, 118. 1, 118. 0, 117. 4, 113. 6, 113. 1, 102. 0, 71. 9, 61. 5, 60. 3, 59. 9, 58. 7, 54. 7, 54. 7, 41. 7, 41. 6, 31. 8, 24. 0, 20. 4, 15. 9, 9. 6. ESI-MS m/z : Calcd. for C4oH39N301OS : 753. 2. Found (M+H+) : 754. 7.

Compound 1711 (from 1311) : lu NMR (300 MHz, CDC13) : 5 6. 43 (s, 1H), 6. 04 (dd, 2H), 5. 70 (s, 1H), 5. 00 (d, 1H), 4. 94- 4. 90 (m, 1H), 4. 59 (bp, 1H), 4. 28 (s, 1H), 4. 24 (d, 1H), 4. 17- 4. 11 (m, 2H), 3. 78 (s, 3H), 3. 46 (d, 1H), 3. 45-3. 39 (m, 2H), 3. 10 (s, 3H), 2. 94-2. 78 (m, 2H), 2. 50-2. 42 (m, 1H), 2. 31 (s, 3H), 2. 29 (s, 3H), 2. 17 (s, 3H), 2. 08-2. 03 (m, 1H), 2. 03 (s, 3H) ; lac NMR (75 MHz, CDC13) : 8 168. 8, 166. 9, 147. 8, 146. 1, 143. 2, 141. 4, 140. 8, 130. 7, 129. 4, 121. 3, 120. 5, 118. 2, 118. 0, 113. 6, 113. 3, 102. 3, 77. 4, 61. 4, 61. 0, 60. 5, 60. 1, 59. 6, 55. 0, 54. 8, 41. 8, 41. 7, 39. 6, 33. 0, 24. 3, 20. 6, 16. 0, 9. 8. ESI-MS m/z : Calcd. for C32H3sN3OllS2 : 701. 2. Found (M+Na+) : 724. 6.

Compound 18a* (from 14a*) 1H NMR (300 MHz, CDC13) : 8 6. 49 (s, 1H), 6. 04 (dd, 2H), 5. 69 (s, 1H), 4. 50-4. 06 (m, 7H), 3. 80 (s, 3H), 3. 53 (d, 1H), 3. 41-3. 38 (m, 1H), 2. 96-2. 87 (m, 1H), 2. 75 (d, 1H), 2. 33-1. 84 (m, 2H), 2. 33 (s, 3H), 2. 30 (s, 3H), 2. 14 (s, 3H), 2. 02 (s, 3H), 1. 94 (s, 3H) ; ESI-MS m/z : Calcd. for C33H35N30IOS : 665. 2. Found (M+H+) : 666. 7.

Example 8 Method H : To a solution of 1 equiv. of 5 in CH3CN (0. 05M) under Argon at room temperature, were added the amine and 3 equiv. of AcOH. After 40 min. 1. 5 equiv. of NaBH3CN were added and the solution was stirred for 40 min. After this time the reaction mixture was diluted with CH2C12, neutralized with NaHC03 and extracted with CH3Cl2. The organic layer was dried with Na2SO4. Flash chromatography gives pure compounds.

Compound 3o (using propyl amine) : 1H NMR (300 MHz, CDCl3 : 8 6. 51 (s, 1H), 6. 02 (dd, 2H), 5. 71 (s, 1H), 5. 01 (d, 1H), 4. 53 (bp, 1H), 4. 24-4. 19 (m, 3H), 4. 10 (dd, 1H), 3. 77 (s, 3H), 3. 41-3. 40 (m, 2H), 3. 17-3. 16 (m, 1H), 3. 00-2. 82 (m, 2H), 2. 46-1. 97 (m, 4H), 2. 29 (s, 3H), 2. 27 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H), 1. 44-1. 25 (m, 2H), 0. 84 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 8 172. 5, 168. 6, 147. 6, 145. 5, 142. 9, 140. 8, 140. 4, 130. 6, 129. 1, 120. 8, 120. 7, 118. 2, 113. 7, 113. 2, 101. 9, 61. 4, 60. 1, 60. 0, 59. 5, 59. 0, 54. 7, 54. 6, 49. 2, 41. 5, 32. 9, 23. 8, 23. 3, 20. 6, 15. 7, 11. 7, 9. 6.

ESI-MS m/z : Calcd. for C34H4oN408S : 664. 3. Found (M+H+) : 665. 3.

Example 9 Method I : To a solution of 1 equiv. of 3b-i, 3k-l, 3q, 3s, 3u-v, 3x-y or 15 in CH3CN/H2O 3 : 2 (0. 009M) were added 30 equiv. of AgN03. After 24 h the reaction was quenched with a mixture 1 : 1 of saturated solutions of brine and NaHC03, stirred for 10 min and diluted and extracted with CH2CI2. The organic layer was dried with Na2SO4.

Chromatography gives pure compounds 4b-i, 4k-l, 4q, 4s, 4u-v, 4x-y or 19.

Compound 4b : tR= 48. 2 min [HPLC, Symmetry 300 C18, 5µm, 250x4. 6 mm, ?, 285 nm, flow= 1. 2 ml/min, temp= 40°C, grad. : CH3CNaq.-NH4OAc (10mM), 1% DEA, pH= 3. 0, 10%-60% (90')] ;1H NMR (300 MHz, CDC13) : 8 6. 53 (s, 1H), 6. 49 (bd, 1H), 6. 02 (dd, 2H), 5. 69 (bp, 1H), 5. 17 (d, 1H), 4. 81 (s, 1H), 4. 52-4. 46 (m, 3H), 4. 16-4. 10 (m, 2H), 3. 74 (s, 3H), 3. 51-3. 48 (m, 1H), 3. 25-3. 20 (m, 1H), 2. 83-2. 80 (m, 2H), 2. 45-2. 40 (m, 1H), 2. 29-2. 02 (m, 1H), 2. 29 (s, 3H), 2. 27 (s, 3H), 2. 15 (s, 3H), 2. 02 (s, 3H) ;"C NMR (75 MHz, CDC13) : 8 168. 8, 168. 6, 156. 8, 156. 3, 155. 7, 147. 4, 145. 7, 142. 9, 141. 1, 140. 9, 131. 2, 129. 7, 120. 8, 120. 7, 117. 9, 114. 9, 112. 7, 101. 9, 81. 4, 62. 0, 60. 1, 57. 7, 57. 6, 56. 0, 54. 8, 52. 9, 42. 2, 41. 3, 29. 7, 23. 6, 20. 5, 15. 6, 9. 6. ESI-MS m/z : Calcd. for C32H34F3N30lOs : 709. 2. Found (M-H20+H) : 692. 2.

Compound 4c : IH NMR (300 MHz, CDC13) : 5 6. 56 (s, 1H), 6. 01 (dd, 2H), 5. 70 (s, 1H), 5. 57 (bd, 1H), 5. 15 (d, 1H), 4. 77 (s, 1H), 4. 61-4. 57 (m, 1H), 4. 50-4. 42 (m, 2H), 4. 15-4. 07 (m, 2H), 3. 77 (s, 3H), 3. 49-3. 47 (m, 1H), 3. 23-3. 15 (m, 1H), 2. 85- 2. 82 (m, 2H), 2. 32-1. 98 (m, 4H), 2. 32 (s, 3H), 2. 28 (s, 3H), 2. 13 (s, 3H), 2. 01 (s, 3H), 1. 65-1. 58 (m, 2H), 0. 96 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 8 171. 8, 170. 5, 147. 9, 145. 6, 143. 0, 141. 0, 140. 8, 131. 6, 128. 8, 121. 0, 120. 7, 118. 9, 115. 3, 101. 8, 81. 5, 61. 6, 60. 3, 57. 8, 57. 6, 56. 0, 55. 0, 51. 9, 42. 0, 41. 3, 38. 3, 32. 6, 23. 7, 20. 5, 18. 9, 16. 1, 13. 8, 9. 6. ESI-MS m/z : Calcd. for C34H4lN301OS : 683. 2. Found (M-H2O+H+) : 666. 3.

Compound 4d :1H NMR (300 MHz, CDCl3) : 8 6. 56 (s, 1H), 6. 02 (dd, 2H), 5. 72 (bs, 1H), 5. 55 (bd, 1H), 5. 15 (d, 1H), 4. 78 (s, 1H), 4. 64-4. 60 (m, 1H), 4. 48-4. 42 (m, 2H), 4. 17-4. 12 (m, 1H), 4. 09 (dd, 1H), 3. 77 (s, 3H), 3. 53-3. 48 (m, 1H), 3. 27-3. 20 (m, 1H), 2. 90-2. 75 (m, 2H), 2. 34-1. 91 (m, 5H), 2. 34 (s, 3H), 2. 28 (s, 3H), 2. 14 (s, 3H), 2. 01 (s, 3H), 0. 98 (d, 3H), 0. 93 (d, 3H) ; ESI-MS m/z : Calcd. for C35Ht3N30ioS : 697. 3. Found (M-H20+H+) : 680. 0.

Compound 4e :'H NMR (300 MHz, CDC13) : 8 6. 56 (s, 1H), 6. 02 (d, 2H), 5. 70 (s, 1H), 5. 55 (bd, 1H), 5. 15 (d, 1H), 4. 77 (s, 1H), 4. 61-4. 55 (m, 1H), 4. 50-4. 42 (m, 2H), 4. 17-4. 14 (m, 1H), 4. 08 (dd, 1H), 3. 77 (s, 3H), 3. 51-3. 48 (m, 1H), 3. 26-3. 19 (m, 1H), 2. 86-2. 79 (m, 2H), 2. 32-1. 98 (m, 4H), 2. 32 (s, 3H), 2. 28 (s, 3H), 2. 15 (s, 3H), 2. 01 (s, 3H), 1. 65-1. 58 (m, 2H), 1. 37-1. 22 (m, 8H), 0. 89 (t, 3H) ; ESI-MS m/z : Calcd. for C38H49N3OIOS : 739. 3. Found (M-H20+H+) : 722. 3.

Compound 4f :'H NMR (300 MHz, CDC13) : 8 6. 56 (s, 1H), 6. 02 (dd, 2H), 5. 70 (s, 1H), 5. 57-5. 53 (bd, 1H), 5. 14 (d, 1H), 4. 77 (s, 1H), 4. 58 (ddd, 1H), 4. 47-4. 43 (m, 2H), 4. 18-4. 13 (m, 1H), 4. 08 (dd, 1H), 3. 77 (s, 3H), 3. 50-3. 46 (m, 1H), 3. 25- 3. 19 (m, 1H), 2. 88-2. 82 (m, 1H), 2. 32-1. 95 (m, 4H), 2. 32 (s, 3H), 2. 28 (s, 3H), 2. 15 (s, 3H), 2. 01 (s, 3H), 1. 40-1. 20 (m, 26H), 0. 88 (t, 3H) ; ESI-MS m/z : Calcd. for C46H65N3OloS : 851. 4. Found (M-H20+H+) : 834. 5.

Compound 4g : 1H NMR (300 MHz, CDCl3) : S 7. 70-7. 67 (m, 2H), 7. 56-7. 45 (m, 3H), 6. 49 (s, 1H), 6. 42 (d, 1H), 6. 03 (dd, 2H), 5. 66 (s, 1H), 5. 20 (d, 1H), 4. 82 (s, 1H), 4. 73 (dt, 1H), 4. 52-4. 45 (m, 2H), 4. 16-4. 10 (m, 2H), 3. 61 (s, 3H), 3. 52 (bd, 1H), 3. 27-3. 22 (m, 1H), 2. 90-2. 85 (m, 2H), 2. 62-2. 56 (m, 1H), 2. 28-1. 92 (m, 1H), 2. 28 (s, 3H), 2. 13 (s, 3H), 2. 03 (s, 3H), 1. 92 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 170. 4, 168. 5, 166. 4, 147. 6, 145. 7, 142. 9, 141. 1, 140. 9, 134. 4, 131. 5, 129. 3, 128. 6, 127. 0, 125. 1, 121. 2, 120. 5, 115. 1, 112. 6, 101. 8, 81. 5, 61. 6, 60. 1, 57. 9, 56. 0, 55. 0, 53. 3, 42. 1, 41. 3, 32. 7, 23. 9, 20. 4, 15. 6, 9. 6 ; ESI-MS m/z : Calcd. for C37H39N3OloS : 717. 2. Found (M-H20+H+) : 699. 9.

Compound 4h :'H NMR (300 MHz, CDC13) : 8 7. 60 (d, 1H), 7. 55-7. 51 (m, 2H), 7. 44-7. 38 (m, 3H), 6. 65 (s, 1H), 6. 25 (d, 1H), 6. 02 (dd, 2H), 5. 80 (d, 1H), 5. 71 (s, 1H), 5. 18 (d, 1H), 4. 79 (s, 1H), 4. 69 (ddd, 1H), 4. 49-4-43 (m, 2H), 4. 16-4. 09 (m, 2H), 3. 68 (s, 3H), 3. 51-3. 49 (m, 1H), 3. 26-3. 20 (m, 1H), 2. 89- 2. 86 (m, 2H), 2. 52-2. 47 (m, 1H), 2. 29-2. 03 (m, 1H), 2. 29 (s, 3H), 2. 27 (s, 3H), 2. 17 (s, 3H), 2. 03 (s, 3H) ;'3C NMR (75 MHz, CDCl3) : # 170. 4, 168. 5, 164. 5, 147. 9, 145. 6, 143. 0, 141. 8, 141. 5, 141. 0, 140. 8, 134. 8, 131. 6, 129. 7, 129. 0, 128. 8, 127. 9, 121. 0, 120. 5, 120. 1, 118. 7, 115. 2, 112. 7, 101. 8, 81. 6, 61. 7, 60. 2, 57. 7, 57. 6, 56. 0, 54. 9, 52. 7, 42. 0, 41. 3, 32. 5, 23. 7, 20. 5, 16. 3, 9. 6. ESI-MS m/z : Calcd. for C39H41N30lOS : 743. 2. Found (M-H20+H+) : 726. 3.

Compound 4i :'H NMR (300 MHz, CDC13) : 8 7. 83 (s, 1H), 7. 65-7. 51 (m, 4H), 6. 65 (s, 1H), 6. 29 (d, 1H), 6. 03 (dd, 2H), 5. 81 (d, 1H), 5. 71 (s, 1H), 5. 18 (d, 1H), 4. 79 (s, 1H), 4. 71-4. 67 (m, 1H), 4. 49-4. 47 (m, 2H), 4. 16-4. 09 (m, 2H), 3. 70 (s, 3H), 3. 51-3. 49 (m, 1H), 3. 23-3. 20 (m, 1H), 2. 88-2. 86 (m, 2H), 2. 47-2. 33 (m, 1H), 2. 30-2. 02 (m, 1H), 2. 30 (s, 3H), 2. 28 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H) ; ESI-MS m/z : Calcd. for C40H40N3F30lOs : 811. 2. Found (M-H20+H') : 794. 2.

Compound 4k : 1H NMR (300 MHz, CDC13) : 8 8. 32 (bp, 1H), 6. 56 (s, 1H), 6. 54 (s, 1H), 6. 01 (dd, 2H), 5. 48 (bd, 1H), 5. 14 (d, 1H), 4. 75 (s, 1H), 4. 68-4. 63 (m, 1H), 4. 55-4. 45 (m, 3H), 4. 33 (dd, 1H), 4. 22 (bp, 1H), 4. 05 (dd, 1H), 3. 80 (s, 3H), 3. 53-3. 45 (m, 1H), 3. 22-3. 13 (m, 1H), 3. 10-3. 02 (m, 1H), 2. 94-2. 84 (m, 3H), 2. 66 (d, 1H), 2. 34-1. 91 (m, 4H), 2. 34 (s, 3H), 2. 30 (s, 3H), 2. 10 (bs, 3H), 2. 01 (bs, 3H), 1. 75-1. 22 (m, 6H) ; 13C NMR (75 MHz, CDC13) : 8 171. 0, 170. 4, 163. 7, 148. 9, 145. 5, 142. 7, 141. 1, 140. 5, 131. 8, 128. 8, 122. 2, 120. 3, 112. 6, 101. 7, 82. 0, 62. 1, 60. 1, 59. 7, 57. 2, 56. 4, 55. 7, 55. 3, 51. 2, 41. 9, 41. 2, 41. 1, 34. 3, 32. 9, 27. 8, 27. 5, 24. 8, 23. 9, 20. 7, 16. 2, 9. 6 ; ESI-MS m/z : Calcd. for C4oH49N5Ol, S2 : 840. 0. Found (M-H2O+) : 822. 3.

Compound 41 : 1H NMR (300 MHz, CDC13) : 8 6. 58 (s, 1H), 6. 02 (dd, 2H), 5. 82-5. 72 (bm, 2H), 5. 15 (d, 1H), 4. 79 (bs, 1H), 4. 57-4. 45 (m, 3H), 4. 22-4. 15 (bp, 1H), 4. 11 (dd, 1H), 3. 78 (s, 3H), 3. 59-3. 49 (bp, 1H), 3. 30-3. 23 (bp, 1H), 2. 91- 2. 83 (m, 2H), 2. 68-2. 45 (m, 4H), 2. 35-2. 02 (m, 2H), 2. 32 (s, 3H), 2. 29 (s, 3H), 2. 17 (s, 3H), 2. 01 (s, 3H) ; ESI-MS m/z : Calcd. for C34H39N3O12S: 713. 2. Found (M-H20+H+) : 696. 2.

Compound 4q : IH NMR (300 MHz, CDC13) : 8 6. 55 (s, 1H), 6. 07 (d, 1H), 6. 02 (d, 2H), 5. 75 (s, 1H), 5. 64 (d, 1H), 5. 15 (d, 1H), 4. 78 (s, 1H), 4. 67-4. 62 (m, 1H), 4. 50-4. 45 (m, 2H), 4. 14-4. 09 (m, 3H), 3. 80 (s, 3H), 3. 51-3. 47 (m, 1H), 3. 25-3. 20 (m, 1H), 2. 85-2. 82 (m, 2H), 2. 50 (s, 3H), 2. 29-1. 98 (m, 3H), 2. 29 (s, 3H), 2. 13 (s, 3H), 2. 02 (s, 3H), 1. 98 (s, 3H), 1. 06 (d, 3H), 0. 97 (d, 3H) ; ESI-MS m/z : Calcd. for C37H46N4011S : 754. 3. Found (M-H2O+H+) : 737. 3.

Compound 4s ESI-MS m/z : Calcd. for C3gH49N5O11 S : 783. 3.

Found (M+) : 766. 3.

Compound 4u : ESI-MS m/z : Calcd. for C47H55N50i2S : 914. 0. Found (M-H2O+H+) : 897. 0.

Compound 4v :'H NMR (300 MHz, CDC13) : 8 6. 70 (bp, 1H), 6. 54 (s, 1H), 6. 02 (d, 2H), 5. 16 (d, 1H), 4. 79 (s, 1H), 4. 55- 4. 48 (m, 3H), 4. 15-4. 07 (m, 2H), 3. 77 (s, 3H), 3. 52-3. 49 (m, 1H), 3. 32-3. 21 (m, 2H), 2. 85-2. 80 (m, 2H), 2. 31-2. 02 (m, 2H), 2. 31 (s, 3H), 2. 29 (s, 3H), 2. 12 (s, 3H), 2. 02 (s, 3H), 1. 26 (d, 3H) ; ESI-MS m/z : Calcd. for C33H40N4010S : 684. 2. Found (M-H2O+H+) : 667. 2.

Compound 4x : ESI-MS m/z : Calcd. for C42H46N4011S : 814. 9. Found (M-H20+H+) : 797. 9.

Compound 4y :'H NMR (300 MHz, CDC13) : 5 7. 77-7. 67 (m, 4H), 7. 42-7. 28 (m, 4H), 6. 55 (s, 1H), 6. 18-6. 06 (bp, 1H), 6. 02 (dd, 2H), 6. 03-5. 86 (m, 1H), 5. 70 (bs, 1H), 5. 58 (bd, 1H), 5. 35-5. 20 (m, 2H), 5. 15 (d, 1H), 4. 79 (s, 1H), 4. 60-4. 55 (m, 3H), 4. 46 (d, 1H), 4. 20-4. 11 (m, 4H), 3. 73 (s, 3H), 3. 49-3. 47 (m, 1H), 3. 21-3. 15 (m, 2H), 3. 06-2. 70 (m, 6H), 2. 38-2. 11 (m, 2H), 2. 38 (s, 3H), 2. 24 (s, 3H), 2. 11 (s, 3H), 2. 02 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 169. 8, 168. 9, 147. 8, 145. 8, 145. 7, 143. 0, 141. 0, 140. 8, 132. 5, 131. 4, 127. 5, 127. 1, 127. 0, 125. 0, 125. 0, 120. 6, 119. 8, 117. 9, 115. 1, 101. 8, 81. 4, 65. 8, 61. 6, 60. 3, 57. 8, 55. 9, 55. 0, 54. 4, 52. 4, 47. 0, 42. 1, 41. 3, 37. 2, 36. 5, 33. 3, 23. 6, 20. 4, 16. 1, 9. 6. ESI-MS m/z : Calcd. for C5IHs4N40i2S2 : 978. 3.

Found (M-H20+H+) : 961. 3.

Compound 19 :'H NMR (300 MHz, CDC13) : 8 6. 58 (s, 1H), 6. 01 (dd, 2H), 5. 71 (s, 1H), 5. 16 (d, 1H), 4. 76 (s, 1H), 4. 47- 4. 43 (m, 2H), 4. 15-4. 11 (m, 1H), 4. 08 (dd, 1H), 4. 01-3. 96 (m, 1H), 3. 78 (s, 3H), 3. 49-3. 45 (m, 1H), 3. 21-3. 17 (m, 1H), 2. 88- 2. 83 (m, 2H), 2. 35-2. 02 (m, 2H), 2. 31 (s, 3H), 2. 29 (s, 3H), 2. 17 (s, 3H), 2. 02 (s, 3H) ; ESI-MS m/z : Calcd. for C3oH34N20) oS : 614. 2. Found (M-H20+H') : 597. 1.

Example 10 Method J : To a solution of 1 equiv. of 3a, 3n-p, 3r, 3t, 17a, 17cc, 17e-f, 17h, 1711 or 18a* in THF/H2O 4 : 1 (0. 03M) were added 5 equiv. of CuBr. After 24 h the reaction was diluted with CH2C12, washed with saturated solutions of NaHC03 and brine, and the organic layer dried with Na2SO4. Chromatography gives pure compounds 4a, 4n-p, 4r, 4t, 21a, 21c, 21e-f, 21h, 2111 or 22a*.

Compound 4a : top-24. 6 min [HPLC, Symmetry 300 C18, 5 m, 250x4. 6 mm, k= 285 nm, flow= 1. 2 ml/min, temp= 40°C, grad. : CH3CNaq.-NH4OAc (10mM), 1% DEA, pH= 3. 0, 10%-60% (90')] ; 1H NMR (300 MHz, CDC13) : 8 6. 57 (s, 1H), 6. 02 (dd, 2H), 5. 79 (bs, 1H), 5. 60 (bd, 1H), 5. 15 (d, 1H), 4. 77 (s, 1H), 4. 56 (ddd, 1H), 4. 46-4. 43 (m, 2H), 4. 15 (dd, 1H), 4. 09 (dd, 1H), 3. 77 (s, 3H), 3. 49-3. 47 (m, 1H), 3. 23-3. 20 (m, 1H), 2. 91-2. 76 (m, 2H), 2. 31-2. 11 (m, 2H), 2. 31 (s, 3H), 2. 28 (s, 3H), 2. 14 (s, 3H), 2. 01 (s, 3H), 1. 89 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 170. 4, 168. 8, 168. 5, 148. 0, 145. 6, 143. 0, 141. 0, 140. 7, 131. 5, 128. 8, 120. 9, 120. 6, 118. 9, 115. 2, 112. 7, 101. 8, 81. 5, 61. 6, 60. 2, 57. 7, 57. 4, 55. 9, 55. 0, 52. 1, 52. 0, 41. 3, 32. 4, 23. 6, 22. 9, 20. 5, 16. 1, 9. 5. ESI-MS m/z : Calcd. for C32H37N3OloS : 655. 2. Found (M-H20+H+) : 638. 1.

Compound 4n :'H NMR (300 MHz, CDC13) : 8 7. 29-7. 21 (m, 5H), 6. 39 (s, 1H), 5. 99 (dd, 2H), 5. 66 (s, 1H), 5. 16 (d, 1H), 4. 74 (s, 1H), 4. 52 (d, 1H), 4. 44 (bp, 1H), 4. 12 (d, 1H), 4. 03 (dd, 1H), 3. 73 (s, 3H), 3. 64 (dd, 2H), 3. 48-3. 47 (m, 1H), 3. 21- 3. 17 (m, 2H), 2. 95 (d, 1H), 2. 84-2. 75 (m, 1H), 2. 35-2. 30 (m, 1H), 2. 30 (s, 3H), 2. 16 (s, 3H), 2. 07-2. 01 (m, 1H), 2. 01 (s, 3H), 1. 93 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 172. 6, 168. 6, 147. 6, 145. 4, 142. 8, 140. 9, 140. 8, 140. 2, 131. 3, 130. 8, 129. 1, 128. 8, 128. 2, 126. 8, 121. 4, 120. 9, 117. 9, 115. 6, 112. 4, 101. 7, 81. 8, 60. 9, 60. 1, 59. 5, 57. 8, 57. 6, 56. 1, 54. 9, 51. 4, 41. 8, 41. 3, 33. 3, 23. 6, 20. 6, 15. 2, 9. 6. ESI-MS m/z : Calcd. for C37H41N309S : 703. 3. Found (M-H20+H+) : 686. 7.

Compound 4o : IH NMR (300 MHz, CDC13) : 8 6. 53 (s, 1H), 6. 00 (dd, 2H), 5. 69 (bp, 1H), 5. 14 (d, 1H), 4. 74 (s, 1H), 4. 44- 4. 49 (m, 2H), 4. 13 (bd, 1H), 4. 04 (dd, 1H), 3. 78 (s, 3H), 3. 49- 3. 47 (m, 1H), 3. 22-3. 16 (m, 2H), 2. 96-2. 75 (m, 2H), 2. 51- 2. 02 (m, 4H), 2. 29 (s, 3H), 2. 28 (s, 3H), 2. 15 (s, 3H), 2. 02 (s, 3H), 1. 42-1. 25 (m, 2H), 0. 86 (t, 3H) ; ESI-MS m/z : Calcd. for C33H41N309S : 655. 3.

Found (M-H20+H+) : 638. 3.

Compound 4p : IH NMR (300 MHz, CDC13) : 8 6. 67 (bp. 1H), 6. 52 (s, 1H), 6. 02 (dd, 2H), 5. 67 (bp, 1H), 5. 16 (d, 1H), 4. 80 (s, 1H), 4. 63-4. 60 (m, 1H), 4. 49 (d, 1H), 4. 45 (bp, 1H), 4. 16 (d, 1H), 4. 08 (dd, 1H), 3. 77 (s, 3H), 3. 52-3. 9 (m, 1H), 3. 25- 3. 20 (m, 1H), 3. 00 (d, 1H), 2. 85-2. 82 (m, 2H), 2. 32-2. 02 (m, 3H), 2. 32 (s, 3H), 2. 29 (s, 3H), 2. 11 (s, 3H), 2. 02 (s, 3H), 0. 99 (d, 3H), 0. 81 (d, 3H) ; ESI-MS m/z : Calcd. for C35H44N40 oS : 712. 3. Found (M-H20+H+) : 695. 2 Compound 4r :'H NMR (300 MHz, CDC13) : b 7. 59 (d, 1H), 7. 49-7. 46 (m, 2H), 7. 36-7. 34 (m, 3H), 6. 58 (s, 1H), 6. 42 (d, 1H), 6. 34 (d, 1H), 6. 01 (dd, 2H), 5. 79 (s, 1H), 5. 69 (d, 1H), 5. 15 (d, 1H), 4. 78 (s, 1H), 4. 70-4. 65 (m, 1H), 4. 50-4. 47 (m, 2H), 4. 28 (dd, 1H), 4. 15 (d, 1H), 4. 10 (dd, 1H), 3. 81 (s, 3H), 3. 49 (d, 1H), 3. 25-3. 22 (m, 1H), 2. 85-2. 83 (m, 2H), 2. 57 (s, 3H), 2. 28-2. 14 (m, 3H), 2. 28 (s, 3H), 2. 14 (s, 3H), 2. 01 (s, 3H), 1. 10 (d, 3H), 1. 01 (d, 3H) ; 13C NMR (75 MHz, CDC13) : 8 170. 1, 170. 0, 168. 6, 165. 2, 148. 0, 145. 7, 143. 2, 141. 12, 140. 84, 134. 8, 131. 2, 129. 9, 129. 6, 128. 8, 127. 8, 120. 8, 120. 7, 120. 6, 118. 4, 115. 3, 112. 7, 101. 8, 81. 5, 61. 7, 60. 4, 57. 8, 57. 7, 57. 5, 56. 0, 55. 0, 52. 0, 42. 2, 41. 3, 32. 7, 32. 6, 23. 7, 20. 5, 19. 2, 18. 0, 16. 4, 9. 6. ESI-MS m/z : Calcd. for C44H5oNaOIIS : 842. 9. Found (M-H2O+H+) : 825. 3.

Compound 4t :'H NMR (300 MHz, CDC13) : b 6. 54 (s, 1H), 6. 49 (d, 1H), 6. 21-6. 16 (m, 1H), 6. 07-5. 96 (m, 2H), 5. 78 (s, 1H), 5. 63 (bd, 1H), 5. 14 (d, 1H), 4. 81, 4. 78 (2s, 1H), 4. 64- 4. 60 (m, 1H), 4. 53-4. 08 (m, 6H), 3. 78, 3. 7s (2s, 3H), 3. 65- 3. 45 (m, 1H), 3. 33-3. 22 (m, 1H), 2. 90-2. 66 (m, 2H), 2. 48 (s, 3H), 2. 28-1. 99 (m, 3H), 2. 28 (s, 3H), 2. 16, 2. 13 (2s, 3H), 2. 01 (s, 3H), 1. 99 (s, 3H), 1. 37, 1. 34 (2d, 3H), 1. 08- 1. 03 (m, 3H), 0. 96-0. 93 (m, 3H) ; 13C NMR (75 MHz, CDC13) : 8 171. 8, 170. 1, 169. 6, 169. 5, 169. 5, 168. 7, 147. 9, 145. 7, 143. 1, 141. 0, 140. 8, 131. 3, 129. 6, 120. 7, 120. 4, 118. 5, 115. 2, 112. 6, 101. 8, 81. 4, 61. 6, 60. 4, 60. 3, 57. 7, 57. 6, 57. 5, 55. 9, 54. 9, 51. 9, 48. 9, 48. 9, 42. 2, 41. 3, 32. 5, 32. 3, 23. 6, 23. 2, 20. 5, 19. 2, 19. 1, 18. 6, 17. 7, 17. 6, 16. 3, 9. 6ESI-MS m/z : Calcd. for C4oHslNsOI2S : 825. 3. Found (M-H2O+H+) : 808. 3.

Compound 21a : IH NMR (300 MHz, CDC13) : 8 6. 52 (s, 1H), 6. 01 (dd, 2H), 5. 64 (s, 1H), 5. 13 (d, 1H), 5. 00 (t, 1H), 4. 76 (s, 1H), 4. 48-4. 45 (m, 2H), 4. 15-4. 12 (m, 1H), 4. 02 (dd, 1H), 3. 79 (s, 3H), 3. 50-3. 47 (m, 1H), 3. 22-3. 17 (m, 1H), 2. 82-2. 79 (m, 2H), 2. 30-1. 98 (m, 2H), 2. 30 (s, 3H), 2. 29 (s, 3H), 2. 15 (s, 3H), 2. 02 (s, 3H), 1. 98 (s, 3H) ; ESI-MS m/z : Calcd. for C32H36N201 : 656. 2. Found (M-H20+H+) : 639. 2.

Compound 21c :'H NMR (300 MHz, CDC13) : 8 6. 45 (s, 1H), 6. 01 (dd, 2H), 5. 63 (s, 1H), 5. 13 (d, 1H), 5. 03 (t, 1H), 4. 77 (s, 1H), 4. 50-4. 48 (m, 2H), 4. 14 (bd, 1H), 4. 02 (dd, 1H), 3. 79 (s, 3H), 3. 51-3. 49 (bd, 1H), 3. 21-3. 12 (m, 1H), 2. 85-2. 75 (m, 2H), 2. 31-2. 02 (m, 4H), 2. 31 (s, 3H), 2. 29 (s, 3H), 2. 13 (s, 3H), 2. 02 (s, 3H), 1. 66-1. 56 (m, 2H), 0. 97 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 5 172. 4, 168. 6, 166. 9, 147. 1, 145. 6, 142. 8, 141. 1, 131. 8, 128. 6, 125. 1, 121. 4, 115. 4, 101. 8, 81. 5, 71. 6, 61. 2, 60. 2, 58. 2, 57. 9, 56. 1, 55. 0, 41. 8, 41. 4, 36. 0, 31. 6, 23. 9, 20. 4, 18. 3, 15. 8, 13. 7, 9. 6.

ESI-MS m/z : Calcd. for C34H4oN20nS : 684. 2. Found (M-H20+H+) : 667. 2.

Compound 21e : 1H NMR (300 MHz, CDC13) : 8 6. 49 (s, 1H), 6. 01 (dd, 2H), 5. 63 (s, 1H), 5. 13 (d, 1H), 5. 02 (t, 1H), 4. 76 (s, 1H), 4. 47-4. 46 (m, 2H), 4. 13 (dd, 1H), 4. 02 (dd, 1H), 3. 79 (s, 3H), 3. 50-3. 49 (m, 1H), 3. 21-3. 19 (m, 1H), 2. 81-2. 78 (m, 2H), 2. 30-2. 02 (m, 4H), 2. 30 (s, 3H), 2. 29 (s, 3H), 2. 13 (s, 3H), 2. 02 (s, 3H), 1. 62-1. 54 (m, 2H), 1. 32-1. 25 (m, 8H), 0. 90 (t, 3H) ; 13C NMR (75 MHz, CDC13) : 8 172. 6, 168. 6, 166. 9, 147. 1, 145. 5, 142. 8, 141. 1, 141. 0, 131. 7, 128. 6, 121. 4, 117. 9, 115. 4, 112. 3, 101. 8, 81. 5, 71. 5, 61. 2, 60. 2, 58. 1, 57. 9, 56. 1, 55. 0, 41. 8, 41. 4, 33. 9, 31. 7, 31. 6, 29. 1, 28. 9, 24. 7, 23. 9, 22. 6, 20. 4, 15. 8, 14. 1, 9. 6. ESI-MS m/z : Calcd. for C38H48N2OS : 740. 3. Found (M-H2O+H+) : 723. 2.

Compound 21f : 1H NMR (300 MHz, CDC13) : 8 6. 50 (s, 1H), 6. 01 (dd, 2H), 5. 63 (s, 1H), 5. 13 (d, 1H), 5. 02 (t, 1H), 4. 77 (bs, 1H), 4. 50-4. 48 (m, 2H), 4. 16-4. 12 (m, 1H), 4. 02 (dd, 1H), 3. 79 (s, 3H), 3. 51-3. 49 (m, 1H), 3. 22-3. 19 (m, 1H), 2. 82-2. 77 (m, 2H), 2. 37-2. 02 (m, 7H), 2. 30 (s, 3H), 2. 29 (s, 3H), 2. 02 (s, 3H), 1. 65-1. 59 (m, 2H), 1. 40-1. 16 (m, 24H), 0. 88 (t, 3H) ; ESI-MS m/z : Calcd. for C46H64N2OloS : 852. 4.

Found (M-H2O+H+) : 835. 4.

Compound 21h :'H NMR (300 MHz, CDC13) : 8 7. 64 (d, 1H), 7. 55-7. 52 (m, 2H), 7. 42-7. 40 (m, 3H), 6. 54 (s, 1H), 6. 30 (d, 1H), 6. 02 (dd, 2H), 5. 65 (s, 1H), 5. 19-5. 16 (m, 2H), 4. 79 (s, 1H), 4. 50-4. 49 (m, 2H), 4. 15 (d, 1H), 4. 05 (dd, 1H), 3. 79 (s, 3H), 3. 51 (d, 1H), 3. 22-3. 19 (m, 1H), 2. 89-2. 76 (m, 2H), 2. 45-2. 41 (m, 1H), 2. 31 (s, 3H), 2. 26 (s, 3H), 2. 13 (s, 3H), 2. 13-2. 03 (m, 1H), 2. 03 (s, 3H) ; 13C NMR (75 MHz, CDC13) : S 168. 6, 166. 9, 165. 7, 147. 1, 145. 5, 145. 4, 142. 8, 141. 1, 141. 0, 134. 6, 131. 9, 130. 3, 128. 9, 128. 1, 121. 3, 117. 6, 115. 4, 112. 3, 101. 8, 81. 5, 72. 0, 61. 2, 60. 3, 58. 2, 57. 9, 56. 1, 55. 0, 41. 9, 41. 4, 31. 8, 23. 9, 20. 4, 15. 9, 9. 6. ESI-MS m/z : Calcd. for C3gH40N2OolS : 744. 2. Found (M-H2O+H+) : 727. 2.

Compound 21II: 1H NMR (300 MHz, CDC13) : 8 6. 45 (s, 1H), 6. 01 (dd, 2H), 5. 68 (s, 1H), 5. 12 (d, 1H), 4. 92 (t, 1H), 4. 78 (s, 1H), 4. 53-4. 42 (m, 2H), 4. 15-4. 03 (m, 2H), 3. 78 (s, 3H), 3. 51- 3. 48 (m, 1H), 3. 24-3. 20 (m, 1H), 3. 10 (s, 3H), 2. 83-2. 78 (m, 2H), 2. 50-2. 42 (m, 1H), 2. 31 (s, 3H), 2. 30 (s, 3H), 2. 17 (s, 3H), 2. 08-2. 03 (m, 1H), 2. 03 (s, 3H) ; ESI-MS m/z : Calcd. for C3lH36N2012S2 : 692. 2. Found (M-H20+H+) : 675. 2.

Compound 22a* :'H NMR (300 MHz, CDC13) : 8 6. 50 (s, 1H), 6. 02 (dd, 2H), 5. 67 (s, 1H), 4. 73 (bp, 1H), 4. 71 (s, 1H), 4. 48- 4. 38 (m, 4H), 4. 12-4. 10 (m, 1H), 3. 80 (s, 3H), 3. 61-3. 59 (m, 1H), 3. 22-3. 18 (m, 1H), 2. 89-2. 80 (m, 1H), 2. 70 (d, 1H), 2. 33-1. 86 (m, 2H), 2. 33 (s, 3H), 2. 30 (s, 3H), 2. 12 (s, 3H), 2. 01 (s, 3H), 1. 94 (s, 3H) ; ESI-MS m/z : Calcd. for C32H36N2OIlS : 656. 2. Found (M-H20+H') : 639. 2.

Example 11 Method K : A solution of 7 in CH2C12/H2O/TFA 2 : 1 : 4 (0. 013M) was stirred for 15 min at RT. Then the reaction was diluted with CH2C12, neutralized with a saturated solution of NaHCO3 and Na2C03 and extracted with CH2Cl2. The organic layer was dried with Na2SO4. Flash chromatography (CH2Cl2/MeOH) gives pure 2p.

Compound 2p :'H NMR (300 MHz, CDC13) : 5 6. 93 (bp. 1H), 6. 72 (s, 1H), 6. 05 (dd, 2H), 5. 15 (dd, 2H), 5. 03 (d, 1H), 4. 66- 4. 63 (m, 1H), 4. 54 (bp, 1H), 4. 35 (d, 1H), 4. 32 (s, 1H), 4. 23 (d, 1H), 4. 17 (dd, 1H), 3. 75 (s, 3H), 3. 56 (s, 3H), 3. 49-3. 42 (m, 2H), 3. 04 (d, 1H), 2. 93-2. 90 (m, 2H), 2. 28-2. 03 (m, 3H), 2. 28 (s, 6H), 2. 14 (s, 3H), 2. 03 (s, 3H), 0. 97 (d, 3H), 0. 77 (d, 3H) ; ESI-MS m/z : Calcd. for C38H47N501oS : 765. 3. Found (M+H+) : 766. 3.

Example 12 Method L : To a solution of 10 in CH3CN (0. 03M) were added 2 equiv. of NaCNBH3 and 4 equiv. of AcOH. After 4 h the reaction was diluted with CH2C12, neutraliced with a saturated solution of NaHC03 and extracted with CH2C12. The organic layer was dried with Na2S04. Flash chromatography (Hex/EtOAc 2 : 1) gives pure compounds.

Compound 11 :'H NMR (300 MHz, CDC13) : 8 6. 77 (s, 1H), 6. 03 (dd, 2H), 5. 17 (dd, 2H), 5. 04 (d, 1H), 4. 53 (bp, 1H), 4. 34 (d, 1H), 4. 27 (s, 1H), 4. 20 (d, 1H), 4. 19 (dd, 1H), 4. 01 (bdd, 1H), 3. 77 (s, 3H), 3. 57 (s, 3H), 3. 55-3. 39 (m, 2H), 2. 94-2. 91 (m, 2H), 2. 30-1. 98 (m, 2H), 2. 30 (s, 3H), 2. 25 (s, 3H), 2. 20 (s, 3H), 2. 03 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 172. 6, 168. 6, 149. 6, 148. 3, 145. 7, 141. 0, 140. 4, 131. 6, 130. 3, 124. 8, 124. 7, 120. 5, 118. 0, 113. 3, 102. 0, 99. 1, 69. 8, 61. 4, 60. 4, 59. 6, 59. 1, 59. 0, 57. 4, 54. 9, 54. 6, 41. 4, 41. 4, 35. 0, 23. 8, 20. 3, 15. 7, 9. 6. ESI-MS m/z : Calcd. for C33H37N3OlOs : 667. 3. Found (M+H+) : 668. 2.

Compound 12* :'H NMR (300 MHz, 45°C, CDC13) : 5 6. 70 (s, 1H), 6. 04 (dd, 2H), 5. 17 (dd, 2H), 4. 88 (bd, 1H), 4. 49 (bs, 1H), 4. 33 (bd, 1H), 4. 27-4. 24 (m, 1H), 4. 24 (s, 1H), 4. 08 (d, 1H), 3. 79 (s, 3H), 3. 60-3. 55 (m, 2H), 3. 56 (s, 3H), 3. 42-3. 39 (m, 1H), 3. 00-2. 91 (m, 1H), 2. 76 (d, 1H), 2. 50-2. 42 (m, 1H), 2. 32 (s, 3H), 2. 27 (s, 3H), 2. 16 (s, 3H), 2. 02 (s, 3H), 1. 66 (dd, 1H) ; ESI-MS m/z : Calcd. for C33H37N3010S : 667. 3. Found (M+H+) : 668. 2.

Example 13 Method M : To a solution of 1 equiv. of 11 for 13a-b or 12* for 14a* in CH2C12 (0-IM) under Argon were added 30 equiv of pyr. Then the reaction was cold to 0°C and 20 equiv. of the anhydride and 5 equiv. of DMAP were added. After 5 min the reaction was warmed to room temperature and stirred for 24 h. After this time it was quenched with NaCl, extracted with CH2C12 and the organic layers dried with Na2S04. Flash chromatography gives pure compounds.

Compound 13a (using AC2O) :'H NMR (300 MHz, CDC13) : 8 6. 70 (s, 1H), 6. 04 (dd, 2H), 5. 17 (dd, 2H), 5. 02-4. 99 (m, 2H), 4. 56 (bp, 1H), 4. 34 (dd, 1H), 4. 27 (s, 1H), 4. 18 (d, 1H), 4. 14 (dd, 1H), 3. 78 (s, 3H), 3. 57 (s, 3H), 3. 46-3. 39 (m, 2H), 2. 90- 2. 87 (m, 2H), 2. 30-1. 96 (m, 2H), 2. 30 (s, 3H), 2. 25 (s, 3H), 2. 17 (s, 3H), 2. 03 (s, 3H), 1. 99 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8169. 7, 167. 1, 148. 9, 148. 2, 145. 9, 141. 2, 140. 6, 130. 7, 130. 7, 125. 3, 124. 6, 120. 8, 118. 1, 113. 5, 113. 1, 102. 0, 99. 2, 71. 6, 61. 4, 60. 0, 59. 9, 59. 2, 58. 7, 57. 4, 55. 0, 54. 6, 41. 5, 31. 6, 23. 9, 20. 3, 20. 2, 15. 8, 9. 6. ESI-MS m/z : Calcd. for C35H39N3OlsS : 709. 6. Found (M+H+) : 710. 2.

Compound 13b (using (F3CCO) 20) :'H NMR (300 MHz, CDC13) : 8 6. 67 (s, 1H), 6. 04 (dd, 2H), 5. 17 (dd, 2H), 5. 10 (bt, 1H), 5. 02 (d, 1H), 4. 62 (bp, 1H), 4. 34-4. 32 (m, 2H), 4. 19-4. 15 (m, 2H), 3. 76 (s, 3H), 3. 56 (s, 3H), 3. 47 (d, 1H), 3. 44-3. 41 (m, 1H), 2. 94-2. 77 (m, 2H), 2. 47-2. 37 (m, 1H), 2. 31 (s, 3H), 2. 23 (s, 3H), 2. 17 (s, 3H), 2. 07-2. 04 (m, 1H), 2. 04 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 168. 7, 164. 9, 148. 7, 148. 2, 145. 9, 141. 2, 140. 7, 131. 6, 130. 3, 125. 7, 124. 0, 120. 6, 118. 0, 113. 3, 102. 1, 99. 2, 74. 7, 61. 4, 60. 5, 60. 0, 59. 1, 59. 2, 58. 7, 57. 4, 54. 9, 54. 6, 41. 7, 41. 5, 31. 1, 23. 9, 20. 2, 15. 5, 9. 6. ESI-MS m/z : Calcd. for C35H36 F3N3011S : 763. 2. Found (M+H+) : 764. 2.

Compound 14a* (using Ac2O) :'H NMR (300 MHz, CDC13) : 8 6. 71 (s, 1H), 6. 05 (dd, 2H), 5. 16 (dd, 2H), 4. 65-4. 10 (m, 7H), 3. 79 (s, 3H), 3. 57-3. 54 (m, 1H), 3. 56 (s, 3H), 3. 43-3. 40 (m, 1H), 2. 97-2. 88 (m, 1H), 2. 78 (d, 1H), 2. 33-1. 82 (m, 2H), 2. 32 (s, 3H), 2. 27 (s, 3H), 2. 15 (s, 3H), 2. 03 (s, 3H), 1. 94 (s, 3H) ; ESI-MS m/z : Calcd. for C3sH39N3OIlS : 709. 6. Found (M+H+) : 710. 7.

COMPOUNDS 23 AND 24 : Compound 23 :'H NMR (300 MHz, CDC13) : 8 6. 52 (s, 1H), 5. 95 (dd, 2H), 4. 97 (d, 1H), 4. 42 (d, 1H), 4. 28 (bs, 2H), 4. 15 (d, 1H), 4. 05 (dd, 1H), 3. 78 (s, 3H), 3. 51-3. 50 (m, 1H), 3. 40- 3. 39 (m, 1H), 3. 27 (t, 1H), 2. 91-2. 89 (m, 2H), 2. 38-2. 36 (m, 2H), 2. 28 (s, 3H), 2. 17 (s, 3H), 2. 14 (s, 3H) ; 13C NMR (75 MHz, CDC13) : 8 173. 9, 148. 1, 146. 2, 146. 1, 142. 8, 136. 2, 130. 4, 129. 5, 120. 8, 118. 2, 112. 7, 112. 7, 107. 7, 101. 3, 61. 1, 60. 9, 60. 4, 59. 4, 58. 8, 54. 6, 54. 6, 53. 5, 43. 3, 41. 4, 33. 0, 23. 9, 15. 7, 8. 7 ; ESI-MS m/z : Calcd. for C29H32N407S : 580. 2. Found (M+H+) : 581. 3.

Compound 24 :'H NMR (300 MHz, CDC13) : 8 6. 40 (s, 1H), 6. 02 (d, 2H), 5. 00 (d, 1H), 4. 46 (bp, 1H), 4. 24 (s, 1H), 4. 21- 4. 14 (m, 3H), 3. 39-3. 37 (m, 2H), 3. 29 (t, 1H), 2. 93-2. 78 (m, 2H), 2. 31-2. 03 (m, 2H), 2. 31 (s, 3H) j 2. 25 (bs, 3H), 2. 14 (s, 6H) ; 13C NMR (75 MHz, CDC13) : 8 173. 6, 168. 9, 145. 6, 145. 3, 140. 9, 140. 2, 139. 3, 126. 1, 123. 9, 120. 2, 119. 7, 118. 1, 117. 7, 113. 6, 113. 3, 101. 9, 61. 3, 60. 3, 59. 1, 59. 1, 54. 7, 54. 6, 53. 3, 41. 9, 41. 4, 33. 0, 23. 5, 20. 5, 16. 8, 9. 6 ; ESI-MS m/z : Calcd. for C3oH32N408S : 608. 2. Found (M+H+) : 609. 3.

Example 14 Compound Int-14 To a solution of Int-2 (21. 53 g, 39. 17 ml) in ethanol (200 ml), tert-butoxycarbonyl anhydride (7. 7 g, 35. 25 ml) was added and the mixture was stirred for 7 h at 23 °C.

Then, the reaction was concentrated in vacuo and the residue was purified by flash column chromatography (Si02, hexane : ethyl acetate 6 : 4) to give Int-14 (20. 6 g, 81 %) as a yellow solid.

Rf : 0. 52 (ethyl acetate : CHCl3 5 : 2).

'H NMR (300 MHz, CDC13) : 5 6. 49 (s, 1H), 6. 32 (bs, 1H), 5. 26 (bs, 1H), 4. 60 (bs, 1H), 4. 14 (d, J= 2. 4 Hz, 1H), 4. 05 (d, J= 2. 4 Hz, 1H), 3. 94 (s, 3H), 3. 81 (d, J= 4. 8 Hz, 1H), 3. 7 (s, 3H), 3. 34 (br d, J= 7. 2 Hz, 1H), 3. 18-3. 00 (m, 5H), 2. 44 (d, J= 18.3 HZ, 1H), 2. 29 (s, 3H), 2. 24 (s, 3H), 1. 82 (s, 3H), 1. 80-1. 65 (m, 1H), 1. 48 (s, 9H), 0. 86 (d, J= 5. 7 Hz, 3H) 3C NMR (75 MHz, CDC13) : 8 185. 5, 180. 8, 172. 7, 155. 9, 154. 5, 147. 3, 143. 3, 141. 5, 135. 3, 130. 4, 129. 2, 127. 5, 120. 2, 117. 4, 116. 9, 80. 2, 60. 7, 60. 3, 58. 5, 55. 9, 55. 8, 54. 9, 54. 4, 50. 0, 41. 6, 40. 3, 28. 0, 25. 3, 24. 0, 18. 1, 15. 6, 8. 5.

ESI-MS m/z : Calcd. forC34H43N508 : 649. 7. Found (M+H) + : 650. 3.

Example 15 Compound Int-15 To a stirred solution of Int-14 (20. 6 g, 31. 75 ml) in CH3CN (159 ml), diisopropylethylamine (82. 96 ml, 476. 2 ml), methoxymethylene bromide (25. 9 ml, 317. 5 ml) and dimethylaminopyridine (155 mg, 1. 27 ml) were added at 0 °C. The mixture was stirred at 23 °C for 24h. The reaction was quenched at 0 °C with aqueous 0. 1N HC1 (750 ml) (pH = 5), and extracted with CH2CI2 (2 x 400 ml). The organic phase was dried (sodium sulphate) and concentrated in vacuo. The residue was purified by flash column chromatography (Si02, gradient hexane : ethyl acetate 4 : 1 to hexane : ethyl acetate 3 : 2) to give Int-15 (17. 6 g, 83 %) as a yellow solid.

Rf : 0. 38 (hexane : ethyl acetate 3 : 7). tH NMR (300 MHz, CDC13) : 8 6. 73 (s, 1H), 5. 35 (bs, 1H), 5. 13 (s, 2H), 4. 50 (bs, 1H), 4. 25 (d, J= 2. 7 Hz, 1H), 4. 03 (d, J= 2. 7 Hz, 1H), 3. 97 (s, 3H), 3. 84 (bs, 1H), 3. 82-3. 65 (m, 1H), 3. 69 (s, 3H), 3. 56 (s, 3H), 3. 39-3. 37 (m, 1H), 3. 20-3. 00 (m, 5H), 2. 46 (d, J= 18 Hz, 1H), 2. 33 (s, 3H), 2. 23 (s, 3H), 1. 85 (s, 3H), 1. 73-1. 63 (m, 1H), 1. 29 (s, 9H), 0. 93 (d, J= 5. 1 Hz, 3H) 3C NMR (75 MHz, CDC13) : b 185. 4, 180. 9, 172. 4, 155. 9, 154. 5, 149. 0, 148. 4, 141. 6, 135. 1, 131. 0, 129. 9, 127. 6, 124. 4, 123. 7, 117. 3, 99. 1, 79. 3, 60. 7, 59. 7, 58. 4, 57. 5, 56. 2, 55. 9, 55. 0, 54. 2, 50. 0, 41. 5, 39. 9, 28. 0, 25. 2, 24. 0, 18. 1, 15. 6, 8. 5.

ESI-MS m/z : Calcd. for C36H47N509 : 693. 8. Found (M+H) + : 694. 3.

Example 16 Compound Int-16 To a flask containing Int-15 (8 g, 1. 5 ml) in methanol (1. 6 1) an aqueous solution of 1M sodium hydroxide (3. 2 1) was added at 0 °C. The reaction was stirred for 2h at this temperature and then, quenched with 6M HC1 to pH = 5. The mixture was extracted with ethyl acetate (3 x 1 1) and the combined organic layers were dried over sodium sulphate and concentrated in vacuo. The residue was purified by flash column chromatography (Si02, gradient CHC13 to CHCl3 : ethyl acetate 2 : 1) to afford Int-16 (5. 3 mg, 68 %).

Rf : 0. 48 (CH3CN : H20 7 : 3, RP-C18) 'H NMR (300 MHz, CDC13) : 8 6. 73 (s, 1H), 5. 43 (bs, 1H), 5. 16 (s, 2H), 4. 54 (bs, 1H), 4. 26 (d, J= 1. 8 Hz, 1H), 4. 04 (d, J= 2. 7 Hz 1H), 3. 84 (bs, 1H), 3. 80-3. 64 (m, 1H), 3. 58 (s, 3H), 3. 41-3. 39 (m, 1H), 3. 22-3. 06 (m, 5H), 2. 49 (d, J= 18. 6 Hz 1H), 2. 35 (s, 3H), 2. 30-2. 25 (m, 1H), 2. 24 (s, 3H), 1. 87 (s, 3H), 1. 45-1. 33 (m, 1H), 1. 19 (s, 9H), 1. 00 (br d, J= 6. 6 Hz 3H) 3C NMR (75 MHz, CDC13) : 8 184. 9, 180. 9, 172. 6, 154. 7, 151. 3, 149. 1, 148. 6, 144. 7, 132. 9, 131. 3, 129. 8, 124. 5, 123. 7, 117. 3, 116. 8, 99. 1, 79. 4, 59. 8, 58. 6, 57. 7, 56. 2, 55. 6, 54. 9, 54. 5, 50. 1, 41. 6, 40. 1, 28. 0, 25. 3, 24. 4, 18. 1, 15. 7, 8. 0.

ESI-MS m/z : Calcd. for C3sH4sNso9 : 679. 7. Found (M+H) + : 680. 3.

Example 17 Compound Int-17 To a degassed solution of compound Int-16 (1. 8 g, 2. 64 ml) in DMF (221 ml) 10 % Pd/C (360 mg) was added and stirred under H2 (atmospheric pressure) for 45 min. The reaction was filtered through celite under argon, to a flask containing anhydrous Cs2C03 (2. 58 g, 7. 92 ml). Then, bromochloromethane (3. 40 ml 52. 8 ml), was added and the tube was sealed and stirred at 100 °C for 2h. The reaction was cooled, filtered through a pad of celite and washed with CH2C12. The organic layer was concentrated and dried (sodium sulphate) to afford Int-17 as a brown oil that was used in the next step with no further purification.

Rf : 0. 36 (hexane : ethyl acetate 1 : 5, Si02).

'H NMR (300 MHz, CDC13) : 8 6. 68 (s, 1H), 6. 05 (bs, 1H), 5. 90 (s, 1H), 5. 79 (s, 1H), 5. 40 (bs, 1H), 5. 31-5. 24 (m, 2H), 4. 67 (d, J= 8. 1 Hz, 1H), 4. 19 (d, J= 2. 7 Hz, 1H), 4. 07 (bs, 1H), 4. 01 (bs, 1H), 3. 70 (s, 3H), 3. 67 (s, 3H), 3. 64-2. 96 (m, 5H), 2. 65 (d, J=18. 3 Hz, 1H), 2. 33 (s, 3H), 2. 21 (s, 3H), 2. 04 (s, 3H), 2. 01-1. 95 (m, 1H), 1. 28 (s, 9H), 0. 87 (d, J= 6. 3 Hz, 3H) 3C NMR (75 MHz, CDC13) : 8 172. 1, 162. 6, 154. 9, 149. 1, 145. 7, 135. 9, 130. 8, 130. 7, 125. 1, 123. 1, 117. 8, 100. 8, 99. 8, 76. 6, 59. 8, 59. 2, 57. 7, 57. 0, 56. 7, 55. 8, 55. 2, 49. 5, 41. 6, 40. 1, 36. 5, 31. 9, 31. 6, 29. 7, 28. 2, 26. 3, 25. 0, 22. 6, 18. 2, 15. 8, 14. 1, 8. 8.

ESI-MS m/z : Calcd. for C36H47NsOs : 693. 34. Found (M+H) + : 694. 3.

Example 18 Compound Int-18 I u) OMe u) OMe 0 Me 0 Me O, Me p, Me OH I o I Me, N YMe Me i N-Me AIIyIBr, Cs2C03 N y NH DMF, 1h, 23°C O e- Me CN Nu nu "YY0° me O ""-18 To a flask containing a solution of Int-17 (1. 83 g, 2. 65 ml) in DMF (13 ml), Cs2C03 (2. 6 g, 7. 97 ml), and allyl bromide (1. 15 ml, 13. 28 ml) were added at 0° C. The resulting mixture was stirred at 23 °C for lh. The reaction was filtered through a pad of celite and washed with CH2Cl2. The organic layer was dried and concentrated (sodium sulphate).

The residue was purified by flash column chromatography (Si02, CHC13 : ethyl acetate 1 : 4) to afford Int-18 (1. 08 mg, 56 %) as a white solid.

Rf : 0. 36 (CHCl3 : ethyl acetate 1 : 3).

'H NMR (300 MHz, CDC13) : 8 6. 70 (s, 1H), 6. 27-6. 02 (m, 1H), 5. 94 (s, 1H), 5. 83 (s, 1H), 5. 37 (dd, My-1. 01 Hz, J2= 16. 8 Hz, 1H), 5. 40 (bs, 1H), 5. 25 (dd, J= 1. 0 Hz, J2= 10. 5 Hz, 1H), 5. 10 (s, 2H), 4. 91 (bs, 1H), 4. 25-4. 22 (m, 1H), 4. 21 (d, J= 2. 4 Hz, 1H), 4. 14-4. 10 (m, 1H), 4. 08 (d, J=2. 4 Hz, 1H), 4. 00 (bs, 1H), 3. 70 (s, 3H), 3. 59 (s, 3H), 3. 56- 3. 35 (m, 2H), 3. 26-3. 20 (m, 2H), 3. 05-2. 96 (dd, Jl= 8. 1 Hz, J2=18 Hz, 1H), 2. 63 (d, J=18 Hz, 1H), 2. 30 (s, 3H), 2. 21 (s, 3H), 2. 09 (s, 3H), 1. 91-1. 80 (m, 1H), 1. 24 (s, 9H), 0. 94 (d, J= 6. 6 Hz, 3H) 3C NMR (75 MHz, CDC13) : 8 172. 0, 154. 8, 148. 8, 148. 6, 148. 4, 144. 4, 138. 8, 133. 7, 130. 9, 130. 3, 125. 1, 124. 0, 120. 9, 117. 8, 117. 4, 112. 8, 112. 6, 101. 1, 99. 2, 73. 9, 59. 7, 59. 3, 57. 7, 56. 9, 56. 8, 56. 2, 55. 2, 40. 1, 34. 6, 31. 5, 28. 1, 26. 4, 25. 1, 22. 6, 18. 5, 15. 7, 14. 0, 9. 2.

ESI-MS m/z : Calcd. for C39H5iN509 : 733. 4. Found (M+H) + : 734. 4.

Example 19 Compound Int-19 i zu OMe \ orme ) OMe OMe O Me i N Me Me i N YMe J/4. 3M HCI/dioxane \ N I/ O 1. 2h, 23 0 \<0 N \-o CN -O CN NH NH -NHO/ Me 0 Me M (1-18 nL 19 To a solution of Int-18 (0. 1 g, 0. 137 ml) in dioxane (2 ml), 4. 2M HCl/dioxane (1. 46 ml) was added and the mixture was stirred for 1. 2h at 23 °C. The reaction was quenched at 0 °C with sat. Aqueous sodium bicarbonate (60 ml) and extracted with ethyl acetate (2x70 ml). The organic layers were dried (sodium sulphate) and concentrated in vacuo to afford Int-19 (267 mg, 95 %) as a white solid that was used in subsequent reactions with no further purification.

Rf : 0. 17 (ethyl acetate : methanol 10 : 1, Si02) 'H NMR (300 MHz, CDC13) : 8 6. 49 (s, 1H), 6. 12-6. 00 (m, 1H), 5. 94 (s, 1H), 5. 86 (s, 1H), 5. 34 (dd, J= 1. 0 Hz, J= 17. 4 Hz, 1H), 5. 25 (dd, J= 1. 0 Hz, J= 10. 2 Hz, 1H), 4. 18- 3. 76 (m, SH), 3. 74 (s, 3H), 3. 71-3. 59 (m, 1H), 3. 36-3. 20 (m, 4H), 3. 01-2. 90 (m, 1H), 2. 60 (d, J= 18. 0 Hz, 1H), 2. 29 (s, 3H), 2. 24 (s, 3H), 2. 11 (s, 3H), 1. 97-1. 86 (m, 1H), 0. 93 (d, J= 8. 7 Hz, 3H) 3C NMR (75 MHz, CDC13) : 8 175. 5, 148. 4, 146. 7, 144. 4, 142. 4, 138. 9, 133. 7, 131. 3, 128. 3, 120. 8, 117. 9, 117. 4, 113. 8, 112. 4, 101. 1, 74. 2, 60. 5, 59. 1, 56. 5, 56. 1, 56. 3, 56. 0, 55. 0, 50. 5, 41. 6, 39. 5, 29. 5, 26. 4, 24. 9, 21. 1, 15. 5, 9. 33.

ESI-MS m/z : Calcd. for C32H39N506 : 589. Found (M+H) + : 590.

Example 20 Compound Int-20 % OMe % OMe 0 HO I Me 0 HO I Me O Me N YMe phenylisothiocyanate \ N 1-1 -O CN 1, 23 C O CN NH NH oWyNH2 o<NHCSNHPh Me Me -19\n-20 To a solution of Int-19 (250 mg, 0. 42 ml) in CH2C12 (1. 5 ml), phenyl isothiocyanate (0. 3 ml, 2. 51 ml) was added and the mixture was stirred at 23° C for lh. The reaction was concentrated in vacuo and the residue was purified by flash column chromatography (Si02, gradient Hexane to 5 : 1 hexane : ethyl acetate) to afford Int-20 (270 mg, 87 %) as a white solid.

Rf : 0. 56 (CHCl3 : ethyl acetate 1 : 4).

'H NMR (300 MHz, CDC13) : 8 8. 00 (bs, 1H), 7. 45-6. 97 (m, 4H), 6. 10 (s, 1H), 6. 08-6. 00 (m, 1H), 5. 92 (s, IH), 5. 89 (s, IH), 5. 82 (s, IH), 5. 40 (dd, J= 1. 5 Hz, J= 17. 1 Hz, 1H), 3. 38 (bs, 1H), 5. 23 (dd, J= 1. 5 Hz, J= 10. 5 Hz, 1H), 4. 42-4. 36 (m, 1H), 4. 19-4. 03 (m, 5H), 3. 71 (s, 3H), 3. 68-3. 17 (m, 4H), 2. 90 (dd, J=7. 8 Hz, J= 18. 3 Hz, 1H), 2. 57 (d, J= 18. 3 Hz, 1H), 2. 25 (s, 3H), 2. 12 (s, 3H), 2. 10 (s, 3H), 1. 90 (dd, J= 12. 3 Hz, J= 16. 5 Hz, 1H), 0. 81 (d, J= 6.9 Hz, 3H).

13C NMR (75 MHz, CDC13) : 5 178. 4, 171. 6, 148. 6, 146. 8, 144. 3, 142. 7, 138. 7, 136. 2, 133. 6, 130. 7, 129. 8, 126. 6, 124. 2, 124. 1, 120. 9, 120. 5, 117. 7, 117. 4, 116. 7, 112. 6, 112. 5, 101. 0, 74. 0, 60. 6, 59. 0, 57. 0, 56. 2, 56. 1, 55. 0, 53. 3, 41. 4, 39. 7, 26. 3, 24. 8, 18. 3, 15. 5, 9. 2.

ESI-MS m/z : Calcd. for C39H44N606S : 724. 8 Found (M+H) + : 725. 3.

Example 21 Compound Int-21 OMe % OMe OMe Me I HO/Me N ; Me O """rM o rT O N JI/4. 2N HCI in Dioxano Me po 0 30 min., 23 OC-I N-Me NHCO --O l CN NHCSNHPH \ » ° NH2CN mye Me nE-20 ìi-21 To a solution of Int-20 (270 mg, 0. 37 ml) in dioxane (1 ml), 4. 2N HCl/dioxane (3. 5 ml) was added and the reaction was stirred at 23 °C for 30 min. Then, ethyl acetate (20 ml) and H20 (20 ml) were added and the organic layer was decanted. The aqueous phase was basified with saturated aqueous sodium bicarbonate (60 ml) (pH = 8) at 0 °C and then, extracted with CH2Cl2 (2 x 50 ml). The combined organic extracts were dried (sodium sulphate), and concentrated in vacuo. The residue was purified by flash column chromatography (Si02, ethyl acetate : methanol 5 : 1) to afford compound Int-21 (158 mg, 82%) as a white solid.

Rf : 0. 3 (ethyl acetate : methanol 1 : 1).

'H NMR (300 MHz, CDC13) : 8 6. 45 (s, 1H), 6. 12-6. 03 (m, 1H), 5. 91 (s, 1H), 5. 85 (s, 1H), 5. 38 (dd, Jl= 1. 2 Hz, J2= 17. 1 Hz, 1H), 5. 24 (dd, J/= 1. 2 Hz, J2= 10. 5 Hz, 1H), 4. 23-4. 09 (m, 4H), 3. 98 (d, J= 2. 1 Hz, 1H), 3. 90 (bs, 1H), 3. 72 (s, 3H), 3. 36-3. 02 (m, 5H), 2. 72-2. 71 (m, 2H), 2. 48 (d, J= 18. 0 Hz, 1H), 2. 33 (s, 3H), 2. 22 (s, 3H), 2. 11 (s, 3H), 1. 85 (dd, J, = 11. 7 Hz, J2= 15. 6 Hz, 1H)).

13C NMR (75 MHz, CDC13) : 8 148. 4, 146. 7, 144. 4, 142. 8, 138. 8, 133. 8, 130. 5, 128. 8, 121. 5, 120. 8, 118. 0, 117. 5, 116. 9, 113. 6, 112. 2, 101. 1, 74. 3, 60. 7, 59. 9, 58. 8, 56. 6, 56. 5, 55. 3, 44. 2, 41. 8, 29. 7, 26. 5, 25. 7, 15. 7, 9. 4.

ESI-MS m/z : Calcd. for C29H34N405 : 518. 3. Found (M+H) + : 519. 2.

Example 22 Compound Int-22 OMe OMe HO, Me HO, Me 'o 0 Me I TrocCl, py, CHZCIz Me N-Me N-,'Me-10°C, 1 h. I N J Me Nez zoo CN <-O CN NH2 NHTroc Int-21 It) 22 To a solution of Int-21 (0. 64 g, 1. 22 ml) in CH2C12 (6. 13 ml), pyridine (0. 104 ml, 1. 28 ml) and 2, 2, 2-trichloroethyl chloroformate (0. 177 ml, 1. 28 ml) were added at-10 °C.

The mixture was stirred at this temperature for lh and then, the reaction was quenched by addition of 0. 1N HC1 (10 ml) and extracted with CH2Cl2 (2 x 10 ml). The organic layer was dried over sodium sulphate and concentrated in vacuo. The residue was purified by flash column chromatography (Si02, (hexane : ethyl acetate 1 : 2) to afford Int-22 (0. 84 g, 98%) as a white foam solid.

Rf : 0. 57 (ethyl acetate : methanol 5 : 1).

'H NMR (300 MHz, CDC13) : 8 6. 50 (s, 1H), 6. 10-6. 00 (m, 1H), 6. 94 (d, J= 1. 5 Hz, 1H), 5. 87 (d, J= 1. 5 Hz, 1H), 5. 73 (bs, 1H), 5. 37 (dq, Je= 1. 5 Hz, J2= 17. 1 Hz, 1H), 5. 26 (dq, Je= 1. 8 Hz, J2= 10. 2 Hz, 1H), 4. 60 (d, J= 12 Hz, 1H), 4. 22-4. 10 (m, 4H), 4. 19 (d, J= 12 Hz, 1H), 4. 02 (m, 2H), 3. 75 (s, 3H), 3. 37-3. 18 (m, 5H), 3. 04 (dd, Jl= 8. 1 Hz, J2= 18 Hz, 1H), 2. 63 (d, J= 18 Hz, 1H), 2. 31 (s, 3H), 2. 26 (s, 3H), 2. 11 (s, 3H), 1. 85 (dd, Jl= 12. 3 Hz, J2= 15. 9 Hz, 1H).

3C NMR (75 MHz, CDC13) 5 154. 3, 148. 5, 146. 7, 144. 5, 142. 8, 139. 0, 133. 8, 130. 7, 128. 7, 121. 3, 120. 8, 117. 8, 117. 7, 116. 8, 112. 7, 101. 2, 77. 2, 74. 3, 60. 7, 59. 9, 57. 0, 56. 4, 55. 3, 43. 3, 41. 7, 31. 6, 26. 4, 25. 3, 22. 6, 15. 9, 14. 1, 9. 4.

ESI-MS m/z : Calcd. for C32H35C13N407 : 694. 17. Found (M+H) + : 695. 2.

Example 23 Compound Int-23 % OMe 8 OMe OMe OMe BrMOM, CH3CN, DIPEA ))) LDMAP, 30°C, 10h.))) 0 0 \-O CN t~O CN NHTroc NHTroc 22 Int,-23 To a solution of Int-22 (0. 32 g, 0. 46 ml) in CH3CN (2. 33 ml), diisopropylethylamine (1. 62 ml, 9. 34 ml), bromomethyl methyl ether (0. 57 ml, 7. 0 ml) and dimethylaminopyridine (6 mg, 0. 046 ml) were added at 0 °C. The mixture was heated at 30 °C for lOh. Then, the reaction was diluted with dichloromethane (30 ml) and poured in an aqueous solution of HCI at pH = 5 (10 ml). The organic layer was dried over sodium sulphate and the solvent was eliminated under reduced pressure to give a residue which was purified by flash column chromatography (Si02, hexane : ethyl acetate 2 : 1) to afford Int-23 (0. 304 g, 88%) as a white foam solid.

Rf : 0. 62 (hexane : ethyl acetate 1 : 3).

'H NMR (300 MHz, CDC13) : 8 6. 73 (s, 1H), 6. 10 (m, 1H), 5. 94 (d, J= 1. 5 Hz, 1H), 5. 88 (d, J= 1. 5 Hz, 1H), 5. 39 (dq, Jl= 1. 5 Hz, J2= 17. 1 Hz, 1H), 5. 26 (dq, Jl= 1. 8 Hz, J2= 10. 2 Hz, 1H), 5. 12 (s, 2H), 4. 61 (d, J= 12 Hz, 1H), 4. 55 (t, J= 6. 6 Hz, 1H), 4. 25 (d, J= 12 Hz, 1H), 4. 22-4. 11 (m, 4H), 4. 03 (m, 2H), 3. 72 (s, 3H), 3. 58 (s, 3H), 3. 38-3. 21 (m, SH), 3. 05 (dd, J= 8. 1 Hz, J2= 18 Hz, 1H), 2. 65 (d, J= 18 Hz, 1H), 2. 32 (s, 3H), 2. 23 (s, 3H), 2. 12 (s, 3H), 1. 79 (dd, J/= 12. 3 Hz, J2= 15. 9 Hz, 1H) ; 3C NMR (75 MHz, CDC13) 8 154. 3, 148. 6, 148. 4, 144. 5, 139. 0, 133. 6, 130. 6, 130. 1, 125. 07, 124. 7, 124. 0, 121. 1, 117. 7, 112. 6, 101. 2, 99. 2, 77. 2, 74. 4, 74. 1, 59. 8, 59. 8, 57. 7, 57. 0, 56. 8, 56. 68, 55. 3, 43. 2, 41. 5, 26. 4, 25. 2, 15. 9, 9. 3.

ESI-MS m/z : Calcd. for C34H39C13N408 : 738. 20. Found (M+H) + : 739. 0.

Example 24 Compound Int-24 OMe OMe MOMO/Me MOMO, Me AcOHaq, Zn Me, N YMe 7h, 23°C Me N-Me 0 0 = O I N W I Nw/' -O CN -O CN NHTroc NH2 \-0 CN\0 5N In-23 lnl.-24 To a suspension of Int-23 (0. 304 g, 0. 41 ml) in 90% aqueous acetic acid (4 ml), powder zinc (0. 2 g, 6. 17 ml) was added and the reaction was stirred for 7 hour at 23 °C. The mixture was filtered through a pad of celite which was washed with CH2C12. The organic layer was washed with an aqueous sat. solution of sodium bicarbonate (pH = 9) (15 ml) and dried over sodium sulphate. The solvent was eliminated under reduced pressure to give Int-24 (0. 191 g, 83%) as a white solid.

Rf : 0. 3 (ethyl acetate : methanol 5 : 1).

'H NMR (300 MHz, CDC13) : 8 6. 68 (s, 1H), 6. 09 (m, 1H), 5. 90 (d, J= 1. 5 Hz, 1H), 5. 83 (d, J= 1. 5 Hz, 1H), 5. 39 (dq, J>= 1. 5 Hz, J2= 17. 1 Hz, 1H), 5. 25 (dq, J= 1. 5 Hz, J2= 10. 2 Hz, 1H), 5. 10 (s, 2H), 4. 22-4. 09 (m, 3H), 3. 98 (d, J= 2. 4 Hz, 1H), 3. 89 (m, 1H), 3. 69 (s, 3H), 3. 57 (s, 3H), 3. 37-3. 17 (m, 3H), 3. 07 (dd, Jl= 8. 1 Hz, J2= 18 Hz, 1H), 2. 71 (m, 2H), 2. 48 (d, J= 18 Hz, 1H), 2. 33 (s, 3H), 2. 19 (s, 3H), 2. 17 (s, 3H), 1. 80 (dd, J>= 12. 3 Hz, J2= 15. 9 Hz, 1H) 13C NMR (75 MHz, CDC13) : 8 148. 5, 148. 2, 144. 3, 138. 7, 133. 7, 130. 7, 129. 9, 125. 0, 123. 9, 121. 3, 117. 9, 117. 5, 113. 6, 112. 0, 101. 0, 99. 2, 74. 0, 59. 8, 59. 7, 58. 8, 57. 6, 57. 0, 56. 2, 55. 2, 44. 2, 41. 5, 31. 5, 26. 4, 25. 6, 22. 5, 16. 7, 14. 0, 9. 2.

ESI-MS m/z : Calcd. for C3 H38N406 : 562. 66. Found (M+H) + : 563. 1.

Example 25 Compound Int-25 To a solution of Int-24 (20 mg, 0. 035 ml), in H20 (0. 7 ml) and THF (0. 7 ml), NaN02 (12 mg, 0. 17 ml) and 90% aqueous AcOH (0. 06 ml) were added at 0 °C and the mixture was stirred at 0 °C for 3h. After dilution with CH2C12 (5 ml), the organic layer was washed with water (1 ml), dried over sodium sulphate and concentrated in vacuo. The residue was purified by flash column chromatography (Si02, hexane : ethyl acetate 2 : 1) to afford Int-25 (9. 8 mg, 50%) as a white solid.

Rf : 0. 34 (hexane : ethyl acetate 1 : 1).

'H NMR (300 MHz, CDC13) : # 6. 71 (s, 1H), 6. 11 (m, 1H), 5. 92 (d, J= 1. 5 Hz, 1H), 5. 87 (d, J= 1. 5 Hz, 1H), 5. 42 (dq, J= 1. 5 Hz, J2= 17. 1 Hz, 1H), 5. 28 (dq,./7= 1. 5 Hz, J2= 10. 2 Hz, 1H), 5. 12 (s, 2H), 4. 26-4. 09 (m, 3H), 4. 05 (d, J= 2. 4 Hz, 1H), 3. 97 (t, J= 3. 0 Hz, 1H), 3. 70 (s, 3H), 3. 67-3. 32 (m, 4H), 3. 58 (s, 3H), 3. 24 (dd, J1=2. 7 Hz, J2= 15. 9 Hz, 1H), 3. 12 (dd, Jl= 8. 1 Hz, J2= 18. 0 Hz, 1H), 2. 51 (d, J= 18 Hz, 1H), 2. 36 (s, 3H), 2. 21 (s, 3H), 2. 12 (s, 3H), 1. 83 (dd, J, = 12. 3 Hz, J2= 15. 9 Hz, 1H) 13C NMR (75 MHz, CDC13) 8 148. 7, 148. 4, 138. 9, 133. 7, 131. 1, 129. 4, 125. 1, 123. 9, 120. 7, 117. 6, 117. 5, 113. 2, 112. 3, 101. 1, 99. 2, 74. 0, 63. 2, 59. 8, 59. 7, 57. 9, 57. 7, 57. 0, 56. 5, 55. 2, 41. 6, 29. 6, 26. 1, 25. 6, 22. 6, 15. 7, 9. 2.

ESI-MS m/z : Calcd. for C31H37N3O7 : 563. 64. Found (M+H) + : 564. 1.

Example 29 Compound Int-29 HO O g I 2, 2, 2-Trichloroethyl chloroformate NH3CI THF, reflux NHTroc C \cl-29 The starting material (2. 0 g, 5. 90 ml) was added to a suspension of sodium hydride (354 mg, 8. 86 ml) in THF (40 ml) at 23 °C, following the suspension was treated with allyl chloroformate (1. 135 ml, 8. 25 ml) at 23 °C and then refluxed for 3 hours. The suspension was cooled, filtered off, the solid washed with ethyl acetate (100 ml), and the filtrate was concentrated. The oil crude was ground with hexane (100 ml) and kept at 4°C overnight. After, the solvent was decanted and the light yellow slurry was treated with CH2CI2 (20 ml), and precipitated with hexane (100 ml). After 10 minutes, the solvent was decanted again. The operation was repeated until appearing a white solid.

The white solid was filtered off and dried to afford compound Int-29 (1. 80 g, 65%) as a white solid.

'H-NMR (300 MHz, CDC13) : 8 7. 74 (d, J= 7. 5 Hz, 2H), 7. 62 (d, J= 6. 9 Hz, 2H), 7. 33 (t, J= 7. 5 Hz, 2H), 7. 30 (t, J= 6. 3 Hz, 2H), 5. 71 (d, J= 7. 8 Hz, 1H), 4. 73 (d, J= 7. 8 Hz, 2H), 4. 59 (m, 1H), 4. 11 (t, J= 6. 0 Hz, 1H), 3. 17 (dd, J= 6. 0 Hz, J= 2. 7 Hz, 2H), 3. 20 (dd, J= 5. 4 Hz, J= 2. 1 Hz, 2H).

13C_NMR (75 MHz, CDC13) : 8 173. 6, 152. 7, 144. 0, 139. 7, 137. 8, 126. 0, 125. 6, 123. 4, 118. 3, 73. 4, 52. 4, 45. 5, 35. 8, 33. 7.

ESI-MS m/z : Calcd.. for C20Hz8C13NO4S : 474. 8. Found (M+Na) + : 497. 8 Example 30 Compound Int-30 A mixture of compound Int-25 (585 mg, 1. 03 ml) and compound Int-29 (1. 47 mg, 3. 11 ml) were azeotroped with anhydrous toluene (3 x 10 ml). To a solution of Int-25 and Int-29 in anhydrous CH2C12 (40 ml) was added DMAP (633 mg, 5. 18 ml) and EDC-HC1 (994 mg, 5. 18 ml) at 23 °C. The reaction mixture was stirred at 23 °C for 3 hours. The mixture was partitioned with saturated aqueous solution of sodium bicarbonate (50 ml) and the layers were separated. The aqueous layer was washed with CH2C12 (50 ml).

The combined organic layers were dried over sodium sulphate, filtered and concentrated.

The crude was purified by flash column chromatography (ethyl acetate/hexane 1 : 3) to obtain Int-30 (1. 00 g, 95%) as a pale cream yellow solid.

'H-NMR (300 MHz, CDC13) : 8 7. 72 (m, 2H), 7. 52 (m, 2H), 7. 38 (m, 2H), 7. 28 (m, 2H), 6. 65 (s, 1H), 6. 03 (m, 1H), 5. 92 (d, J= 1. 5 Hz, 1H), 5. 79 (d, J= 1. 5 Hz, 1H), 5. 39 (m, 1H), 5. 29 (dq, J= 10. 3 Hz, J= 1. 5 Hz, 1H), 5. 10 (s, 2H), 4. 73 (d, J= 11. 9 Hz, 1H), 4. 66 (d, J= 11. 9 Hz, 1H), 4. 53 (m, 1H), 4. 36-3. 96 (m, 9H), 3. 89 (t, J= 6. 4 Hz, 1H), 3. 71 (s, 3H), 3. 55 (s, 3H), 3. 33 (m, 1H), 3. 20 (m, 2H), 2. 94 (m, 3H), 2. 59 (m, 1H), 2. 29 (s, 3H), 2. 23 (s, 3H), 2. 02 (s, 3H), 1. 83 (dd, J= 16. 0 Hz, J= 11. 9 Hz, 1H).

3C-NMR (75 MHz, CDC13) : 8 169. 7, 154. 0, 148. 8, 148. 4, 145. 7, 144. 5, 140. 9, 139. 0, 133. 7, 130. 9, 130. 6, 127. 6, 127. 0, 124. 8, 124. 6, 124. 1, 120. 8, 119. 9, 118. 2, 117. 7, 117. 3, 112. 7, 112. 1, 101. 3, 99. 2, 74. 7, 73. 9, 64. 4, 59. 8, 57. 7, 57. 0, 56. 8, 55. 4, 53. 3, 46. 7, 41. 4, 36. 5, 34. 7, 31. 5, 26. 4, 24. 9, 22. 6, 15. 7, 14. 0, 9. 1.

ESI-MS m/z : Calcd.. for C51H53Cl3N4OloS : 1020. 4. Found (M+H) + : 1021. 2 Example 31 Compound Int-31 Me Me Me Me 0 Me 0 Me ZOZOS ! zizi 0 J JJOH !] j Me<Me Bu3SnH, (PPh3) 2PdCl2 Me N--Me 'i J l/ AcOH, CH2CI2 --O CN -O 0 CN o,, s NHTroc NHTroc Int-30 Int-31 To a solution of Int-30 (845 mg, 0.82 ml), acetic acid (500 mg, 8.28 ml) and (PPh3) 2PdCl2 (29 mg, 0. 04 ml) in anhydrous CH2C12 20 ml at 23 °C was added, dropwise, Bu3SnH (650 mg, 2. 23 ml). The reaction mixture was stirred at this temperature for 15 min., bubbling was. The crude was quenched with water (50ml) and extracted with CH2C12 (3 x 50 ml). The organic layers were dried over sodium sulphate, filtered and concentrated. The crude was purified by flash column chromatography (ethyl acetate/hexane in gradient from 1 : 5 to 1 : 3) to obtain compound Int-31 (730 mg, 90%) as a pale cream yellow solid.

'H-NMR (300 MHz, CDC13) : 8 7. 72 (m, 2H), 7. 56 (m, 2H), 7. 37 (m, 2H), 7. 30 (m, 2H), 6. 65 (s, 1H), 5. 89 (s, 1H), 5. 77 (s, 1H), 5. 74 (s, 1H), 5. 36 (d, J= 5. 9 Hz, 1H), 5. 32 (d, J= 5. 9 Hz, 1H), 5. 20 (d, J= 9. 0, 1H), 4. 75 (d, J= 12. 0 Hz, 1H), 4. 73 (m, 1H), 4. 48 (d, J= 11. 9 Hz, 1H), 4. 08 (m, 4H), 3. 89 (m, 1H), 3. 86, (t, J= 6. 2 Hz, 1H), 3. 70 (s, 3H), 3. 69 (s, 3H), 3. 38 (m, 1H), 3. 25 (m, 1H), 3. 02-2. 89 (m, 4H), 2. 67 (s, 1H), 2. 61 (s, 1H), 2. 51 (dd, J= 14. 3 Hz, J= 4. 5 Hz, 1H), 2. 29 (s, 3H), 2. 23 (s, 3H), 1. 95 (s, 3H), 1. 83 (m, 1H).

3C-NMR (75 MHz, CDC13) : 8 168. 2, 152. 5, 148. 1, 146. 2, 144. 4, 144. 3, 143. 3, 139. 6, 134. 6, 129. 7, 129. 6, 126. 2, 125. 6, 123. 4, 123. 3, 121. 6, 118. 5, 116. 3, 110. 7, 110. 2, 105. 1, 99. 4, 98. 5, 75. 2, 73. 3, 61. 7, 58. 4, 57. 9, 56. 3, 56. 1, 55. 1, 54. 7, 53. 9, 51. 9, 45. 2, 40. 1, 35. 6, 33. 3, 24. 8, 23. 3., 14. 5, 7. 3.

ESI-MS m/z : Calcd.. for C48H49C13N4010S : 980. 3. Found (M+H) + : 981. 2 Example 32 Compound Int-32 Me Me Me Me 0 Me 0 Me 0 Me Y Me N-Me N-I-Me (PhSeO) 20 N N 0 CN 0CN O S \ O S \ NHTroc NHTroc s NHTroc NHTroc To a solution of Int-31 (310 mg, 0. 32 ml), in anhydrous CH2C12 (15 ml) at-10 °C was added a solution of benzeneseleninic anhydride 70 % (165 mg, 0. 32 ml), in anhydrous CH2C12 (7 ml), via cannula, keeping the temperature at-10 °C. The reaction mixture was stirred at-10 °C for 5 min. A saturated solution of sodium bicarbonate (30 ml) was added at this temperature. The aqueous layer was washed with more CH2C12 (40 ml).

The organic layers were dried over sodium sulphate, filtered and concentrated. The crude was purified by flash column chromatography (ethyl acetate/hexane in gradient from 1 : 5 to 1 : 1) to obtain Int-32 (287 mg, 91%, HPLC : 91. 3%) as a pale cream yellow solid and as a mixture of two isomers (65 : 35) which were used in the next step.

'H-NMR (300 MHz, CDC13) : 8 (Mixture of isomers) 7. 76 (m, 4H), 7. 65 (m, 4H), 7. 39 (m, 4H), 7. 29 (m, 4H), 6. 62 (s, 1H), 6. 55 (s, 1H), 5. 79-5. 63 (m, 6H), 5. 09 (s, 1H), 5. 02 (d, J= 6. 0 Hz, 1H), 4. 99 (d, J= 6. 0 Hz, 1H), 4. 80-4. 63 (m, 6H), 4. 60 (m, 1H), 4. 50 (m, 1H), 4. 38 (d, J= 12. 8 Hz, J= 7. 5 Hz, 1H), 4. 27 (dd, J= 12. 8 Hz, J= 7. 5 Hz, 1H), 4. 16-3. 90 (m, 10H), 3. 84 (s, 3H), 3. 62 (s, 3H), 3. 50 (s, 3H), 3. 49 (s, 3H), 3. 33-2. 83 (m, 14H), 2. 45-2. 18 (m, 2H), 2. 21 (s, 6H), 2. 17 (s, 6H), 1. 77 (s, 6H), 1. 67 (m, 2H).

3C-NMR (75 MHz, CDC13) : 8 (Mixture of isomers) 168. 6, 168. 4, 158. 6, 154. 8, 152. 8, 152. 5, 147. 3, 147. 2, 146. 8, 144. 1, 144. 0, 140. 8, 139. 7, 137. 1, 129. 8, 129. 3, 128. 4, 128. 7, 126. 5, 125. 5, 123. 7, 123. 6, 123. 5, 123. 4, 122. 2, 121. 3, 118. 3, 115. 8, 115. 5, 110. 2, 106. 9, 103. 5, 103. 2, 100. 1, 99. 6, 97. 9, 97. 7, 93. 8, 73. 4, 70. 9, 69. 2, 64. 9, 62. 5, 59. 3, 58. 9, 58. 4, 56. 7, 56. 3, 56. 2, 55. 4, 55. 2, 55. 1, 54. 9, 54. 7, 54. 3, 54. 1, 53. 8, 52. 8, 45. 5, 40. 5, 40. 0, 39. 8, 35. 8, 35. 5, 33. 9, 33. 7, 30. 1, 28. 8, 24. 2, 24. 1, 21. 2, 14. 5, 14. 4, 12. 7, 6. 0, 5. 7.

ESI-MS m/z : Calcd.. for C48H49C13N4O lS : 996. 3. Found (M+H) + : 997. 2 Example 33 Compound Int-33 Me Me ) OMe TrocHN o) OMe Ooze DMSO, Tf20 0 0 Me I 2) DIPEA Ac0 g Me N--Me 3)'BuOH Me 0 N--Me N--Mc 4 I \-O 4) Me NXNMe t° CN s w 5) Ac20, CH2CI2 NHTroc \/ 32 33 The reaction flask was flamed twice, purged vacuum/Argon several times and kept under Argon atmosphere for the reaction. To a solution of DMSO (39. 1 ml, 0. 55 ml, 5 equivalents.) in anhydrous CH2Cl2 (4. 5 ml) was dropwise added triflic anhydride (37. 3 ml, 0. 22 ml, 2 equivalents.) at-78 °C. The reaction mixture was stirred at-78 °C for 20 minutes, then a solution of Int-32 (110 mg, 0. 11 ml, HPLC : 91. 3%) in anhydrous CH2C12 (1 ml, for the main addition and 0. 5 ml for wash) at-78 °C was added, via cannula.

During the addition the temperature was kept at-78 °C in both flasks and the colour changed from yellow to brown. The reaction mixture was stirred at-40 °C for 35 minutes. During this period of time the solution was turned from yellow to dark green.

After this time,'Pr2NEt (153 ml, 0. 88 ml, 8 equivalents.) was dropwise added and the reaction mixture was kept at 0 °C for 45 minutes, the colour of the solution turned to brown during this time. Then t-butanol (41. 6 ml, 0. 44 ml, 4 equivalents.) and 2-'Butyl- 1, 1, 3, 3-tetramethylguanidine (132. 8 ml, 0. 77 ml, 7 equivalents.) were dropwise added and the reaction mixture was stirred at 23 °C for 40 minutes. After this time, acetic anhydride (104. 3 ml, 1. 10 ml, 10 equivalents.) was dropwise added and the reaction mixture was kept at 23 °C for 1 hour more. Then the reaction mixture was diluted with CH2C12 (20ml) and washed with aqueous saturated solution of NH4Cl (SOml), sodium bicarbonate (50ml), and sodium chloride (50ml). The combined organic layers were dried over sodium sulphate, filtered and concentrated. The residue was purified by flash column chromatography (eluent : ethyl acetate/hexane gradient from 1 : 3 to 1 : 2) to afford compound Int-33 (54 mg, 58%) as a pale yellow solid.

'H-NMR (300 MHz, CDC13) : 5 6. 85 (s, 1H), 6. 09 (s, 1H), 5. 99 (s, 1H), 5. 20 (d, J= 5. 8 Hz, 1H), 5. 14 (d, J= 5. 3 Hz, 1H), 5. 03 (m, 1H), 4. 82 (d, J= 12. 2, 1HU), 4. 63 (d, I= 12. 0 Hz, 1H), 4. 52 (m, 1H), 4. 35-4. 17 (m, 4H), 3. 76 (s, 3H), 3. 56 (s, 3H), 3. 45 (m, 2H), 2. 91 (m, 2H), 2. 32 (s, 3H), 2. 28 (s, 3H), 2. 21 (s, 3H), 2. 12 (m, 2H), 2. 03 (s, 3H).

3C-NMR (75 MHz, CDC13) : 8 168. 5, 167. 2, 152. 7, 148. 1, 147. 1, 144. 5, 139. 6, 139. 1, 130. 5, 129. 0, 123. 7, 123. 5, 123. 3, 118. 8, 116. 5, 112. 1, 100. 6, 97. 8, 73. 3, 60. 5, 59. 4, 59. 2, 58. 3, 57. 6, 57. 4, 56. 1, 53. 3, 53. 1, 40. 6, 40. 0, 31. 0, 22. 2, 18. 9, 14. 4, 8. 1.

ESI-MS m/z : Calcd.. for C36H39Cl3N4OllS : 842. 1. Found (M+H) + : 843. 1 Example 34 Compound Int-34 Me TrocHN õ TrocHN 6""'10 Me 0""'1 HO Me Ac0 O S I TMSCI, Nal Ac0 O S Me N-Me CH2CI2, CH3CN Me N-Me I/N I/N tO N tO CN .-33-34 To a solution of Int-33 (12 mg, 0. 014 ml) in dry dichloromethane (1. 2 ml) and HPLC grade acetonitrile (1. 2 ml) was added at 23 °C sodium iodide (21 mg, 0. 14 ml) and freshly distilled (over calcium hydride at atmospheric pressure) trimethylsilyl chloride (15. 4 mg, 0. 14 ml). The reaction mixture turned to orange colour. After 15 min the solution was diluted with dichloromethane (10 ml) and was washed with a freshly aqueous saturated solution of Na2S204 (3 x 10 ml). The organic layer was dried over sodium sulphate, filtered and concentrated. It was obtained compound Int-34 (13 mg, quantitative) as pale yellow solid which was used without further purification.

'H-NMR (300 MHz, CDC13) : 8 6. 85 (s, 1H), 6. 09 (s, 1H), 5. 99 (s, 1H), 5. 27 (d, J= 5. 8 Hz, 1H), 5. 14 (d, J= 5. 3 Hz, 1H), 5. 03 (d, J= 11. 9 Hz, 1H), 4. 82 (d, J= 12. 2, 1H), 4. 63 (d, J= 13. 0 Hz, 1H), 4. 52 (m, 1H), 4. 34 (m, 1H), 4. 27 (bs, 1H), 4. 18 (m, 2H), 3. 76 (s, 3H), 3. 56 (s, 3H), 3. 44 (m, 1H), 3. 42 (m, 1H), 2. 91 (m, 2H), 2. 32 (s, 3H), 2. 28 (s, 3H), 2. 21 (s, 3H), 2. 03 (s, 3H).

ESI-MS m/z : Calcd.. for C34H35N4010S : 798. 1. Found (M+H) + : 799. 1 Example 35 Compound Int-35 TrocHN H N 0 1 HO Me C).""IHO Me Ac0\ s J JJAcOHaq. Ac0\ s J jj Me N-Me Me N-Me I/N I/N t O CN t-O CN \ i-34 \nt-35 To a solution of Int-34 (13 mg, 0. 016 ml) in a mixture of acetic acid/H20 (90 : 10, 1 ml) was added powder Zinc (5. 3 mg, 0. 081 ml) at 23 °C. The reaction mixture was heated at 70 °C for 6 h. After this time, was cooled to 23 °C, diluted with CH2C12 (20 ml) and washed with aqueous saturated solution of sodium bicarbonate (15 ml) and aqueous solution of Et3N (15 ml). The organic layer was dried over sodium sulphate, filtered and concentrated. The residue was purified by flash column chromatography with Silica- NH2 (eluent : ethyl acetate/hexane gradient from 0 : 100 to 50 : 50) to afford compound Int- 35 (6. 8 mg, 77% for two steps) as a pale yellow solid.

'H-NMR (300 MHz, CDC13) : 8 6. 51 (s, 1H), 6. 03 (dd, J= 1. 3 Hz, J= 26. 5 Hz, 2H), 5. 75 (bs, 1H), 5. 02 (d, J= 11. 6 Hz, 1H), 4. 52 (m, 1H), 4. 25 (m, 2H), 4. 18 (d, J= 2. 5 Hz, 1H), 4. 12 (dd, J= 1. 9 Hz, J= 11. 5 Hz, 1H), 3. 77 (s, 3H), 3. 40 (m, 2H), 3. 26 (t, J= 6. 4 Hz, 1H), 2. 88 (m, 2H), 2. 30-2. 10 (m, 2H), 2. 30 (s, 3H), 2. 28 (s, 3H), 2. 18 (s, 3H), 2. 02 (s, 3H).

'3C-NMR (75 MHz, CDC13) : 5 174. 1, 168. 4, 147. 8, 145. 4, 142. 9, 140. 8, 140. 1, 131. 7, 130. 2, 129. 1, 128. 3, 120. 4, 118. 3, 117. 9, 113. 8, 111. 7, 101. 7, 61. 2, 59. 8, 59. 2, 58. 9, 54. 4, 53. 8, 54. 4, 41. 3, 41. 5, 34. 1, 23. 6, 20. 3, 15. 5, 9. 4.

ESI-MS m/z : Calcd.. for C31H34N408S : 622. 7. Found (M+H) + : 623. 2.

Example 36 Compound Int-36 A solution of N-methyl pyridine-4-carboxaldehyde iodide (378 mg, 1. 5 mmol) in anhydrous DMF (5. 8 mL) was treated with anhydrous toluene (2 x 10 mL) to eliminate the amount of water by azeotropic removal of the toluene. A solution of 35 (134 mg, 0. 21 mmol), previously treated with anhydrous toluene (2 x 10 mL), in anhydrous CH2C12 (distilled over CaH2, 7. 2 mL) was added, via cannula, at 23 oC to this orange solution.

The reaction mixture was stirred at 23 °C for 4 hours. After this time DBU (32. 2 L, 0. 21mmol) was dropwise added at 23 °C and it was stirred for 15 minutes at 23 °C. A freshly aqueous saturated solution of oxalic acid (5. 8 mL) was added to the reaction mixture and was stirred for 30 minutes at 23 °C. Then the reaction mixture was cooled to 0 °C and NaHC03 was portionwise added followed by addittion of aqueous saturated solution of NaHC03. The mixture was extracted with Et20. K2C03 was added to the aqueous layer and it was extrated with Et20. The combined organic layers were dried over MgS04 and the solvent was removed under reduced pressure. The crude was purified by flash column chromatography (AcOEt/hexane from 1/3 to 1/1) to afford compound 36 (77 mg, 57%) as pale yellow solid. 1H-NMR (300 MHz, CDC13) : 6. 48 (s, 1H), 6. 11 (d, J= 1. 3 Hz, 1H), 6. 02 (d, J= 1. 3 Hz, 1H), 5. 70 (bs, 1H), 5. 09 (d, J= 11. 3 Hz, 1H), 4. 66 (bs, 1H), 4. 39 (m, 1H), 4. 27 (d, J= 5. 6 Hz, 1H), 4. 21 (d, J= 10. 5 Hz, 1H), 4. 16 (d, J= 2. 6 Hz, 1H), 3. 76 (s, 3H), 3. 54 (d, J= 5. 1 Hz, 1H), 3. 42 (d, J= 8. 5 Hz, 1H), 2. 88-2. 54 (m, 3H), 2. 32 (s, 3H), 2. 24 (s, 3H), 2. 14 (s, 3H), 2. 04 (s, 3H). 13C-NMR (75 MHz, CDC13) : 186. 7, 168. 5, 160. 5, 147. 1, 146. 4, 142. 9, 141. 6, 140. 7, 130. 4, 129. 8, 121. 7 (2C), 120. 0, 117. 8, 117. 1, 113. 5, 102. 2, 61. 7, 61. 4, 60. 3, 59. 8, 58. 9, 54. 6, 41. 6, 36. 9, 29. 7, 24. 1, 20. 3, 15. 8, 14. 1, 9. 6. ESI-MS m/z : Calcd.. for C31H31N309S : 621. 7.

Found (M+H) + : 622. 2.

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