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Title:
APPARATUS AND METHOD FOR HIGH THROUGHPUT PARALLEL NUCLEIC ACID SEQUENCING ON SURFACES OF MICROBEADS
Document Type and Number:
WIPO Patent Application WO/2020/258037
Kind Code:
A1
Abstract:
Method and apparatus for nucleic acid sequencing are provided. The method includes providing a microbead disposed in one reaction well and immobilized with two capturing oligonucleotides with different sequences, immobilizing nucleic acid templates on the microbead via annealing between the templates and the capturing oligonucleotides, amplifying the immobilized nucleic acid templates and producing a population of template clones annealed with sequencing primers. The method further includes sequentially disposing different types of nucleotide trisphosphates, detecting, by ion-sensitive field-effect transistors, ion concentration change in the reaction wells in response to incorporation of one of the nucleotide trisphosphates at 3' end of sequencing primers, when the nucleotide trisphosphates is complementary to a corresponding nucleotide in the template clones, and sequencing the template clones by repeating the sequentially disposing and the detecting. A method for producing single-stranded nucleic acid template clones on a reaction well array is also provided.

Inventors:
DENG DAVID XINGFEI (US)
Application Number:
PCT/CN2019/092820
Publication Date:
December 30, 2020
Filing Date:
June 25, 2019
Export Citation:
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Assignee:
ARDENT BIOMED GUANGDONG CO LTD (CN)
International Classes:
C12Q1/68; C40B10/00
Foreign References:
CN102203282A2011-09-28
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