Title:
APPLICATION OF BRANCHED-CHAIN α-KETOACID DEHYDROGENASE COMPLEX IN PREPARATION OF MALONYL COENZYME A
Document Type and Number:
WIPO Patent Application WO/2021/036901
Kind Code:
A1
Abstract:
An application of a branched-chain α-ketoacid dehydrogenase complex in preparation of malonyl coenzyme A. A method for preparing malonyl coenzyme A by using the branched-chain α-ketoacid dehydrogenase complex. The method comprises: introducing genes encoding the branched-chain α-ketoacid dehydrogenase complex into biological cells, and expressing the genes encoding the branched-chain α-ketoacid dehydrogenase complex to obtain recombinant cells; culturing the recombinant cells to obtain malonyl coenzyme A. The branched-chain α-ketoacid dehydrogenase complex is a complete-set protein consisting of M1) or M2): M1): bkdF, bkdG, bkdH, and lpdA1; and M2): bkdA, bkdB, bkdC, and lpdA1. Experimental results show that by using the branched-chain α-ketoacid dehydrogenase complex, not only malonyl coenzyme A can be prepared, but also a target product using malonyl coenzyme A as an intermediate product can further be prepared.
Inventors:
LIU WEIFENG (CN)
LIU BO (CN)
CUI QIANQIAN (CN)
TAO YONG (CN)
LIU BO (CN)
CUI QIANQIAN (CN)
TAO YONG (CN)
Application Number:
PCT/CN2020/110232
Publication Date:
March 04, 2021
Filing Date:
August 20, 2020
Export Citation:
Assignee:
INST MICROBIOLOGY CAS (CN)
International Classes:
C12P19/32; C12N1/21; C12N9/02; C12N9/10; C12N9/88; C12P7/22; C12P7/42; C12P13/00; C12R1/19
Domestic Patent References:
| WO2012019175A2 | 2012-02-09 |
Foreign References:
| CN1128046A | 1996-07-31 | |||
| CN104284974A | 2015-01-14 | |||
| CN107083412A | 2017-08-22 |
Other References:
CUI QIANQIAN; ZHOU FENGLI; LIU WEIFENG; TAO YONG: "Avermectin biosynthesis: stable functional expression of branched chain α-keto acid dehydrogenase complex fromStreptomyces avermitilisinEscherichia coliby selectively regulating individual subunit gene expression", BIOTECHNOLOGY LETTERS, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, vol. 39, no. 10, 29 June 2017 (2017-06-29), Dordrecht, pages 1567 - 1574, XP036321410, ISSN: 0141-5492, DOI: 10.1007/s10529-017-2389-z
BI HUIPING; BAI YANFEN; CAI TAO; ZHUANG YIBIN; LIANG XIAOMEI; ZHANG XUELI; LIU TAO; MA YANHE: "Engineered short branched-chain acyl-CoA synthesis inE. coliand acylation of chloramphenicol to branched-chain derivatives", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 97, no. 24, 8 October 2013 (2013-10-08), Berlin/Heidelberg, pages 10339 - 10348, XP035328732, ISSN: 0175-7598, DOI: 10.1007/s00253-013-5262-6
M. S. DAVIS, SOLBIATI, CRONAN: "Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 275, no. 37, 8 September 2000 (2000-09-08), pages 28593 - 28598, XP055012857, ISSN: 00219258, DOI: 10.1074/jbc.M004756200
YANG YAPING; LIN YUHENG; LI LINGYUN; LINHARDT ROBERT J.; YAN YAJUN: "Regulating malonyl-CoA metabolism via synthetic antisense RNAs for enhanced biosynthesis of natural products", METABOLIC ENGINEERING, ACADEMIC PRESS, US, vol. 29, 9 April 2015 (2015-04-09), US, pages 217 - 226, XP029590707, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2015.03.018
BI HUIPING; BAI YANFEN; CAI TAO; ZHUANG YIBIN; LIANG XIAOMEI; ZHANG XUELI; LIU TAO; MA YANHE: "Engineered short branched-chain acyl-CoA synthesis inE. coliand acylation of chloramphenicol to branched-chain derivatives", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 97, no. 24, 8 October 2013 (2013-10-08), Berlin/Heidelberg, pages 10339 - 10348, XP035328732, ISSN: 0175-7598, DOI: 10.1007/s00253-013-5262-6
M. S. DAVIS, SOLBIATI, CRONAN: "Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 275, no. 37, 8 September 2000 (2000-09-08), pages 28593 - 28598, XP055012857, ISSN: 00219258, DOI: 10.1074/jbc.M004756200
YANG YAPING; LIN YUHENG; LI LINGYUN; LINHARDT ROBERT J.; YAN YAJUN: "Regulating malonyl-CoA metabolism via synthetic antisense RNAs for enhanced biosynthesis of natural products", METABOLIC ENGINEERING, ACADEMIC PRESS, US, vol. 29, 9 April 2015 (2015-04-09), US, pages 217 - 226, XP029590707, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2015.03.018
Attorney, Agent or Firm:
JEEKAI&PARTNERS (CN)
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