Title:
BIOLOGICALLY ACTIVE SUBSTANCE, METHOD FOR MANUFACTURING SAME AND USE THEREOF AS AN AGENT FOR PROTECTING A BIOLOGICAL TISSUE
Document Type and Number:
WIPO Patent Application WO/2020/128991
Kind Code:
A1
Abstract:
The biologically active substance is obtained or capable of being obtained by alkaline extraction, using an aqueous solution of at least one base in the presence of at least one reducing agent, of at least one macroscopic edible mushroom reduced to powder, to obtain a mixture having a liquid portion containing the soluble extracted materials and a solid portion formed of insoluble solid particles; filtering the resulting mixture to remove the solid portion and optionally clarifying the liquid portion; neutralising the liquid portion using at least one cation exchange resin and optionally filtering/clarifying the liquid portion thus neutralised to obtain the biologically active substance in the aqueous phase; and treating the resulting aqueous phase, the treatment being selected from dilution in water, concentration, dehydration and lyophilisation, to obtain a biologically active substance which is, respectively, more diluted in water, in an aqueous solution that is more concentrated, dehydrated in powder form or lyophilised.
Inventors:
CELERIER JULIEN (FR)
MOINE CHARLOTTE (FR)
FAUGERON-GIRARD CÉLINE (FR)
GLOAGUEN VINCENT (FR)
MOINE CHARLOTTE (FR)
FAUGERON-GIRARD CÉLINE (FR)
GLOAGUEN VINCENT (FR)
Application Number:
PCT/IB2019/061171
Publication Date:
June 25, 2020
Filing Date:
December 20, 2019
Export Citation:
Assignee:
COVERTIS (FR)
UNIV LIMOGES (FR)
UNIV LIMOGES (FR)
International Classes:
A01P1/00; A01N65/00; A01P3/00; A01P7/04; A01P21/00; A61K8/9728; A61Q17/00
Domestic Patent References:
| WO2018069497A1 | 2018-04-19 | |||
| WO1998049331A1 | 1998-11-05 | |||
| WO2011112179A1 | 2011-09-15 | |||
| WO2018069497A1 | 2018-04-19 |
Foreign References:
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| CN1345540A | 2002-04-24 | |||
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| EP1690914A1 | 2006-08-16 |
Other References:
NG TZI BUN: "Mushroom proteins related to host defense", BIOSIS, BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US, 1 January 2005 (2005-01-01), XP002769700, DOI: 10.1615/INTJMEDMUSHR.V7.I12.210
DATABASE WPI Week 201157, Derwent World Patents Index; AN 2011-K73906, XP002794700
LI SHANGSHANG ET AL: "Purification,in vitroantioxidant andin vivoanti-aging activities of soluble polysaccharides by enzyme-assisted extraction fromAgaricus bisporus", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, ELSEVIER BV, NL, vol. 109, 21 December 2017 (2017-12-21), pages 457 - 466, XP085346851, ISSN: 0141-8130, DOI: 10.1016/J.IJBIOMAC.2017.12.108
DUBOISGILLEHAMILTONREBERSSMITH: "Colo-rimetric method for détermination of sugars and relative substances", ANALYTICAL CHEMISTRY, vol. 28, 1956, pages 350 - 356
SMITHGILKERSON: "Quantitation of glycosaminoglycan hexosamine using 3-methyl-2-benzothiazolone hydrazone hydrochloride", ANALYTICAL BIOCHEMISTRY, vol. 98, 1979, pages 478 - 480, XP024829521, DOI: 10.1016/0003-2697(79)90170-2
BRADFORD: "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", ANALYTICAL BIOCHEMISTRY, vol. 72, 1976, pages 248 - 254, XP025650297, DOI: 10.1016/0003-2697(76)90527-3
J.S. SHINDLERR. E. CHILDSW. G. BARDSLEY: "Peroxidase from Human Cervical Mucus: The Isolation and Characterization", EUR. J. BIOCHEM., vol. 65, 1976, pages 325 - 331
DATABASE WPI Week 201157, Derwent World Patents Index; AN 2011-K73906, XP002794700
LI SHANGSHANG ET AL: "Purification,in vitroantioxidant andin vivoanti-aging activities of soluble polysaccharides by enzyme-assisted extraction fromAgaricus bisporus", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, ELSEVIER BV, NL, vol. 109, 21 December 2017 (2017-12-21), pages 457 - 466, XP085346851, ISSN: 0141-8130, DOI: 10.1016/J.IJBIOMAC.2017.12.108
DUBOISGILLEHAMILTONREBERSSMITH: "Colo-rimetric method for détermination of sugars and relative substances", ANALYTICAL CHEMISTRY, vol. 28, 1956, pages 350 - 356
SMITHGILKERSON: "Quantitation of glycosaminoglycan hexosamine using 3-methyl-2-benzothiazolone hydrazone hydrochloride", ANALYTICAL BIOCHEMISTRY, vol. 98, 1979, pages 478 - 480, XP024829521, DOI: 10.1016/0003-2697(79)90170-2
BRADFORD: "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", ANALYTICAL BIOCHEMISTRY, vol. 72, 1976, pages 248 - 254, XP025650297, DOI: 10.1016/0003-2697(76)90527-3
J.S. SHINDLERR. E. CHILDSW. G. BARDSLEY: "Peroxidase from Human Cervical Mucus: The Isolation and Characterization", EUR. J. BIOCHEM., vol. 65, 1976, pages 325 - 331
Attorney, Agent or Firm:
CABINET CHAILLOT (FR)
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