Title:
BIOMEMBRANE FORCE PROBE SYSTEM-BASED SUPER-ALIGNMENT FORCE CLAMP EXPERIMENTAL METHOD
Document Type and Number:
WIPO Patent Application WO/2021/114789
Kind Code:
A1
Abstract:
A biomembrane force probe system-based super-alignment force clamp experimental method. A probe end (1, 2, 3, 7) and a target end (4, 5) are involved in a force clamp experiment. The probe end (1, 2, 3, 7) comprises a probe pellet (3) and red blood cells (2). In the force clamp experiment of a biomembrane force probe, a reference pellet (7) is added to the probe end (1, 2, 3, 7), and by means of the strong interaction between protein molecules, the reference pellet (7) is bonded to the end part of a first micropipette (1) that suctions the red blood cells (2). Moreover, a relative distance (10) between the reference pellet (7) located at a side boundary (9) close to the red blood cells (2) and the probe pellet (3) located at a side boundary (8) close to the red blood cells (2) is tracked in real time, and the movement of the target end (4, 5) is controlled by means of a feedback algorithm to carry out the force clamp experiment. By means of adding the reference pellet (7) and developing a biomembrane force probe system-based 'double-boundary' force tracking mode, the accuracy of the force acting on the molecular bonds is significantly improved, accurate and stable acting forces may be applied to molecular bonds that have slow dissociation rates, and the recording time limit of the binding time of the molecular bonds is significantly prolonged for long-time recording.
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Inventors:
CHEN WEI (CN)
AN CHENYI (CN)
HU WEI (CN)
AN CHENYI (CN)
HU WEI (CN)
Application Number:
PCT/CN2020/115248
Publication Date:
June 17, 2021
Filing Date:
September 15, 2020
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
G01Q60/38
Foreign References:
| CN111122913A | 2020-05-08 | |||
| CN109521227A | 2019-03-26 | |||
| CN101520313A | 2009-09-02 | |||
| CN108051361A | 2018-05-18 | |||
| CN107449939A | 2017-12-08 | |||
| RU2615052C1 | 2017-04-03 |
Other References:
LU SUN; QIAN-HUA CHENG; HUAJIAN GAO; YONG-WEI ZHANG;: "A nonlinear characteristic regime of biomembrane force probe", JOURNAL OF BIOMECHANICS, PERGAMON PRESS, NEW YORK, NY, US, vol. 44, no. 4, 2 November 2010 (2010-11-02), US, pages 662 - 668, XP028146466, ISSN: 0021-9290, DOI: 10.1016/j.jbiomech.2010.11.005
AN CHENYI, FEI PAN-YU; CHEN WEI: "Investigating Protein Dynamic Functions using Single-Molecule Force Spectroscopy", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, ZHONGGUO SHENGWU HUAXUE YU FENZI SHENGWU XUEHUI, BEIJING, CN, vol. 33, no. 10, 1 January 2017 (2017-01-01), CN, pages 965 - 978, XP055821083, ISSN: 1007-7626, DOI: 10.13865/j.cnki.cjbmb.2017.10.01
AN CHENYI, FEI PAN-YU; CHEN WEI: "Investigating Protein Dynamic Functions using Single-Molecule Force Spectroscopy", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, ZHONGGUO SHENGWU HUAXUE YU FENZI SHENGWU XUEHUI, BEIJING, CN, vol. 33, no. 10, 1 January 2017 (2017-01-01), CN, pages 965 - 978, XP055821083, ISSN: 1007-7626, DOI: 10.13865/j.cnki.cjbmb.2017.10.01
Attorney, Agent or Firm:
HANGZHOU QIUSHI PATENT OFFICE CO., LTD. (CN)
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