Title:
CATECHOLAMINE COATING METHOD USING CALCIUM PEROXIDE AND METHOD FOR PREPARING LOW-BINDING CELL CULTURE PLATE USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/015372
Kind Code:
A1
Abstract:
The present invention relates to a catecholamine coating method using calcium peroxide and a method for preparing a low-binding cell culture plate using same and, more specifically, to a catecholamine coating method comprising a step of immersing a substrate to be coated in a catecholamine solution containing calcium peroxide (CaO2) and a method for preparing a low-binding cell culture plate, the method comprising a step of immersing the substrate coated with catecholamine by the coating method in an amine-reactive PEG (mPEG) solution. Developed in the present invention is a surface modification technique for biocompatible materials in which calcium peroxide is used as an oxygen-supplying reagent. The calcium peroxide-mediated oxygen supply promotes the oxidation of dopamine hydrochloride (DA) to rapidly create an amine-rich substrate, so that a low-binding culture plate can be created by a simple immersion procedure. Cells cultured in the low-binding culture plate formed larger and more compact cell spheroids than in other culture plates.
Inventors:
PARK KYUNG MIN (KR)
PARK KI DONG (KR)
AN JEONG AH (KR)
PARK KI DONG (KR)
AN JEONG AH (KR)
Application Number:
PCT/KR2019/017320
Publication Date:
January 28, 2021
Filing Date:
December 09, 2019
Export Citation:
Assignee:
INU RES & BUSINESS FOUNDATION (KR)
UNIV AJOU IND ACADEMIC COOP FOUND (KR)
UNIV AJOU IND ACADEMIC COOP FOUND (KR)
International Classes:
C09D179/02; B05D1/18; C09D7/20; C09D7/61; C12M1/00; C12M1/12; C12N5/00
Foreign References:
| KR20150040136A | 2015-04-14 | |||
| KR20130134844A | 2013-12-10 | |||
| KR20180117855A | 2018-10-30 | |||
| KR20160077979A | 2016-07-04 |
Other References:
AN JEONG AH, KANG JEON IL, PARK KYUNG MIN, PARK KI DONG: "Calcium peroxide-mediated oxygen supply for improved coating efficiency of bio-inspired catecholamine", JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING CHEMISTRY, KOREA, vol. 80, 1 December 2019 (2019-12-01), KOREA, pages 795 - 801, XP055784619, ISSN: 1226-086X, DOI: 10.1016/j.jiec.2019.06.048
Attorney, Agent or Firm:
CHA, Joon Yong (KR)
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