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Title:
CELL CULTURING USING NANOFIBERS
Document Type and Number:
WIPO Patent Application WO/2018/182016
Kind Code:
A1
Abstract:
The present invention provides: a cell carrier that contains nanofibers composed of a water-insoluble polysaccharide, preferably chitin or chitosan nanofibers, the carrier is possibly used as a common carrier for various operations including i) suspension culturing, ii) differentiation induction, iii) transport and storage under non-freezing conditions, and iv) transplantation of adhesive cells, and v) recovery of a physiologically active substance from a culture supernatant; and continuous execution of, using said carrier, a plurality of operations selected from among i) suspension culturing, ii) differentiation induction, iii) transport and storage under non-freezing conditions, and iv) transplantation of adhesive cells, and v) recovery of a physiologically active substance from a culture supernatant.

Inventors:
KANAKI TATSURO (JP)
KIDA KATSUHIKO (JP)
HAYASHI HISATO (JP)
MINAMI MASATAKA (JP)
Application Number:
PCT/JP2018/013977
Publication Date:
October 04, 2018
Filing Date:
March 30, 2018
Export Citation:
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Assignee:
NISSAN CHEMICAL CORP (JP)
International Classes:
C12M3/00; A61K35/28; A61L27/20; A61L27/38; A61L27/40; A61L27/50; A61L27/58; A61P43/00; C12N5/071
Domestic Patent References:
WO2015111686A12015-07-30
WO2015111686A12015-07-30
Foreign References:
JP2015530104A2015-10-15
JP2013541956A2013-11-21
JP2005270891A2005-10-06
JPS5232976B11977-08-25
JP2017068377A2017-04-06
JP2017174237A2017-09-28
Other References:
SUZUKI SHUNTARO ET AL.: "Z17-03-1445 Micro formation of nanochitin-based cell culture scaffold material by inkjet and cell exfoliation using glycolytic enzyme", ABSTRACTS OF THE ANNUAL MEETING OF JAPAN WOOD RESEARCH SOCIETY, vol. 67, 27 February 2017 (2017-02-27), pages 157, XP009516867, ISSN: 0549-3994
See also references of EP 3597729A4
DA SILVA MEIRELLES LCAPLAN AINARDINB., STEM CELLS, vol. 26, no. 9, 2008, pages 2287 - 99
CRISAN MYAP SCASTEILLAL ET AL., CELL STEM CELL, vol. 3, no. 3, 2008, pages 229 - 30
DOMINICI MLE BLANC KMUELLER ISLAPER-CORTENBACH I ET AL., CYTOTHER, vol. 8, no. 4, 2006, pages 315 - 7
HUTLEY L JNEWELL F M ET AL., EUR J CLIN INVEST., vol. 33, no. 7, 2003, pages 574 - 81
YIN YYUAN H ET AL., MOL ENDOCRINOL., vol. 20, no. 2, 2006, pages 268 - 78
RIVAL YSTENNEVIN A ET AL., J PHARMACOL EXP THER., vol. 311, no. 2, 2004, pages 467 - 75
SHIRAKAWA KMAEDA S ET AL., MOL CELL BIOL., vol. 26, no. 16, 2006, pages 6105 - 16
Attorney, Agent or Firm:
TAKASHIMA, Hajime et al. (JP)
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