Title:
CELL HOLDING DEVICE
Document Type and Number:
WIPO Patent Application WO/2020/085423
Kind Code:
A1
Abstract:
[Problem] To provide a cell holding device, in which cells each having a structure close to an actual structure can be cultured and the contractile property of cultured cells can be measured easily.
[Solution] A cell holding device 100 is provided with a substrate 101, a cell culturing/holding member 105, and an operation section 107. Specific cells, e.g., cardiomyocytes, are cultured in a cell culturing section 105d having a specific orientation direction A105. In this manner, cells each having the same structure as those of actual tissue cells can be cultured. The cell culturing section 105d is placed in a through-hole 101a that is a cell culturing/holding member placing space formed in the substrate 101, and the cell culturing section 105d is constrained only in the orientation direction A105 of the cell culturing/holding member 105 and is not constrained in a direction orthogonal to the orientation direction A105. As a result, cultured cells can beat more freely compared with the conventional methods in which the cell culturing section 105d is constrained in all directions. Accordingly, it becomes possible to beat cardiomyocytes greatly compared with the conventional methods.
Inventors:
HORI KOSUKE (JP)
KAWAHARA NORIYUKI (JP)
KAWAHARA NORIYUKI (JP)
Application Number:
PCT/JP2019/041679
Publication Date:
April 30, 2020
Filing Date:
October 24, 2019
Export Citation:
Assignee:
STEM CELL & DEVICE LABORATORY INC (JP)
International Classes:
C12M1/00; C12M1/34; C12M3/00
Domestic Patent References:
| WO2008116189A1 | 2008-09-25 | |||
| WO2016060260A1 | 2016-04-21 | |||
| WO2019167960A1 | 2019-09-06 |
Other References:
HANSEN, KATRINA J. ET AL.: "Development of a Contractile Cardiac Fiber From Pluripotent Stem Cell Derived Cardiomyocytes", FRONTIERS IN CARDIOVASCULAR MEDICINE, vol. 5, no. 52, 11 June 2018 (2018-06-11), pages 1 - 11, XP055709455
PROULX, MEGAN K. ET AL.: "Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH A, vol. 96, 29 November 2010 (2010-11-29), pages 301 - 312, XP055709461
CHROBAK, MEGAN 0. ET AL.: "Design of a Fibrin Microthread-Based Composite Layer for Use in a Cardiac Patch", ACS BIOMATERIALS SCIENCE & ENGINEERING, vol. 3, 1 December 2016 (2016-12-01), pages 1394 - 1403, XP055709464
PROULX, MEGAN K. ET AL.: "Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH A, vol. 96, 29 November 2010 (2010-11-29), pages 301 - 312, XP055709461
CHROBAK, MEGAN 0. ET AL.: "Design of a Fibrin Microthread-Based Composite Layer for Use in a Cardiac Patch", ACS BIOMATERIALS SCIENCE & ENGINEERING, vol. 3, 1 December 2016 (2016-12-01), pages 1394 - 1403, XP055709464
Attorney, Agent or Firm:
Sha patent and Trademark Firm (JP)
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