Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
CHIMERIC DNA POLYMERASE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/127848
Kind Code:
A1
Abstract:
Provided is a chimeric DNA polymerase, comprising: a first peptide segment, which has at least 80% homology with at least part of the amino acid sequence in the N-terminal domain of a KOD DNA polymerase; a second peptide segment, which has at least 80% homology with at least part of the amino acid sequence in the exonucleolytic domain of a Pab DNA polymerase; a third peptide segment, which has at least 80% homology with at least some of the amino acids in the N-terminal domain of the KOD DNA polymerase; a fourth peptide segment, which has at least 80% homology with at least some of the amino acids in the palm domain of a Pfu DNA polymerase; a fifth peptide segment, which has at least 80% homology with at least some of the amino acids in the finger domain of the Pab DNA polymerase; a sixth peptide segment, which has at least 80% homology with at least some of the amino acids in the palm domain of the Pfu DNA polymerase; and a seventh peptide segment, which has at least 80% homology with at least some of the amino acids in the thumb domain of the KOD DNA polymerase.

Inventors:
AN QUN (CN)
GUO MIAOMIAO (CN)
XI FENG (CN)
GUO FEI (CN)
ZHENG YUE (CN)
DONG YULIANG (CN)
ZHANG WENWEI (CN)
Application Number:
PCT/CN2019/127462
Publication Date:
July 01, 2021
Filing Date:
December 23, 2019
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
BGI SHENZHEN (CN)
International Classes:
C12N9/12; C12N15/54; C12P19/34
Domestic Patent References:
WO2004058942A22004-07-15
Foreign References:
CN102257136A2011-11-23
CN108064278A2018-05-22
CN109628424A2019-04-16
EP1925669B12010-12-08
Other References:
CHEN XUAN; ZHAO YANG; QIN MING; WANG ZHIYING; LI PEILING: "Screening and prenatal genetic diagnosis of Fragile X syndrome with Pfu DNA polymerase", CHINESE JOURNAL OF BIRTH HEALTH & HEREDITY, vol. 12, no. 3, 31 December 2004 (2004-12-31), CN, pages 25 - 22, XP009528851, ISSN: 1006-9534
ŚPIBIDA MARTA; KRAWCZYK BEATA; OLSZEWSKI MARCIN; KUR JÓZEF: "Modified DNA polymerases for PCR troubleshooting", JOURNAL OF APPLIED GENETICS: AN INTERNATIONAL JOURNAL OF GENETICS AND BREEDING, vol. 58, no. 1, 28 October 2016 (2016-10-28), pages 133 - 142, XP036137678, ISSN: 1234-1983, DOI: 10.1007/s13353-016-0371-4
Attorney, Agent or Firm:
TSINGYIHUA INTELLECTUAL PROPERTY LLC (CN)
Download PDF:



 
Previous Patent: CAMERA OPTICAL LENS

Next Patent: CAMERA OPTICAL LENS