Title:
COLORIMETRIC DETECTION OF TARGET MATERIAL BASED ON HYDROGEL PARTICLE
Document Type and Number:
WIPO Patent Application WO/2021/075881
Kind Code:
A3
Abstract:
The present invention relates to a colorimetric detection of a target material based on a hydrogel particle. A colorimetric detection of a target material based on a hydrogel particle according to the embodiment of the present invention comprises the steps of: reacting a sample containing the target material with the hydrogel particle, which is loaded on the inside with a probe that specifically binds to the target material; and accumulating and amplifying an insoluble colorimetric material in the hydrogel particle so as to label the target material bound to the probe. Here, the hydrogel particle is made of a polymer network, the probe is loaded by binding to the polymer network, and the insoluble colorimetric material is fixed to the polymer network.
Inventors:
BONG KI WAN (KR)
RHO YOONHO (KR)
MUN SEOK JOON (KR)
LEE HYUN JEE (KR)
KIM JU YEON (KR)
RHO YOONHO (KR)
MUN SEOK JOON (KR)
LEE HYUN JEE (KR)
KIM JU YEON (KR)
Application Number:
PCT/KR2020/014110
Publication Date:
June 10, 2021
Filing Date:
October 15, 2020
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
G01N33/58; G01N33/543; G01N33/68
Foreign References:
| KR20190072829A | 2019-06-26 | |||
| KR20140092029A | 2014-07-23 |
Other References:
DAVID C APPLEYARD, STEPHEN C CHAPIN, RATHI L SRINIVAS , PATRICK S DOYLE: "Bar-coded hydrogel microparticles for protein detection: synthesis, assay and scanning", NATURE PROTOCOLS, vol. 6, no. 11, 20 October 2011 (2011-10-20), pages 1761 - 1774, XP055620989, ISSN: 1754-2189, DOI: 10.1038/nprot.2011.400
LEE HYUN JEE, ROH YOON HO, KIM HYEON UNG, KIM SUN MIN, BONG KI WAN: "Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles", LAB ON A CHIP, vol. 19, no. 1, 23 November 2018 (2018-11-23), pages 111 - 119, XP055816905, ISSN: 1473-0197, DOI: 10.1039/C8LC01160E
LEE HYUN JEE, KIM JU YEON, ROH YOON HO, KIM SUN MIN, BONG KI WAN: "Linker-free antibody conjugation for sensitive hydrogel microparticle-based multiplex immunoassay", ANALYST, vol. 144, no. 22, 2 October 2019 (2019-10-02), pages 6712 - 6720, XP055816907, DOI: 10.1039/C9AN01243E
YOON HO ROH, HYUN JEE LEE , KI WAN BONG: "Microfluidic Fabrication of Encoded Hydrogel Microparticles for Application in Multiplex Immunoassay", BIOCHIP JOURNAL, vol. 13, no. 1, 12 March 2019 (2019-03-12), pages 64 - 81, XP036740168, ISSN: 1976-0280, DOI: 10.1007/s13206-019-3104-z
LEE HYUN JEE, ROH YOON HO, KIM HYEON UNG, KIM SUN MIN, BONG KI WAN: "Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles", LAB ON A CHIP, vol. 19, no. 1, 23 November 2018 (2018-11-23), pages 111 - 119, XP055816905, ISSN: 1473-0197, DOI: 10.1039/C8LC01160E
LEE HYUN JEE, KIM JU YEON, ROH YOON HO, KIM SUN MIN, BONG KI WAN: "Linker-free antibody conjugation for sensitive hydrogel microparticle-based multiplex immunoassay", ANALYST, vol. 144, no. 22, 2 October 2019 (2019-10-02), pages 6712 - 6720, XP055816907, DOI: 10.1039/C9AN01243E
YOON HO ROH, HYUN JEE LEE , KI WAN BONG: "Microfluidic Fabrication of Encoded Hydrogel Microparticles for Application in Multiplex Immunoassay", BIOCHIP JOURNAL, vol. 13, no. 1, 12 March 2019 (2019-03-12), pages 64 - 81, XP036740168, ISSN: 1976-0280, DOI: 10.1007/s13206-019-3104-z
Attorney, Agent or Firm:
JUNG, Eun Youl et al. (KR)
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