Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
COMPOSITIONS AND METHODS FOR RECOVERING FROM BLUE-LIGHT (BL) INDUCED PIGMENTATION, OXIDATIVE STRESS AND INFLAMMATION
Document Type and Number:
WIPO Patent Application WO/2022/157544
Kind Code:
A1
Abstract:
A method for preparing a composition for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation includes the steps of, preparing a BlueLight defence powder from multiple ingredients; subjecting multiple ingredients and brown algae to freeze drying and subsequent grinding to about 80 mesh powder; dissolving the BlueLight Defence in a required amount of PBS to make a solution of 20mg/ml as a stock solution; and, filtering the stock solution through a 20um filter and subsequently diluting the stock solution to a required concentration to treat a plurality of cells. In use, multiple ingredients include Rosmarinus Officinalis (Rosemary) leaves, Stigma maydis, (Cornsilk), Punica granatum (pomegranate) fruit, Cucumis melo var. cantalupensis (Cantaloupe) fruit, and, Camellia sinensis (White Tea leaf).

Inventors:
MOMAND HOSSAY (GB)
ABDUL LATIF JULIANA DATO (SG)
Application Number:
PCT/IB2021/050488
Publication Date:
July 28, 2022
Filing Date:
January 22, 2021
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
JLWH PTE LTD (SG)
International Classes:
A61K8/9789; A61K8/9711; A61K8/9794; A61Q19/00; A61Q19/02
Foreign References:
CN110075024A2019-08-02
CN112105421A2020-12-18
US20200237626A12020-07-30
CN111297791A2020-06-19
CN110917094A2020-03-27
Download PDF:
Claims:
9

Claims

1 . A method for preparing a composition for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation, said method comprising the steps of: preparing a BlueLight defence powder from a plurality of ingredients; subjecting said plurality of ingredients and brown algae to freeze drying and subsequent grinding to about 80 mesh powder; dissolving said BlueLight Defence in a required amount of PBS to make a solution of 20mg/ml as a stock solution; and, filtering said stock solution through a 20um filter and subsequently diluting said stock solution to a required concentration to treat a plurality of cells.

2. The method as claimed in Claim 1 , wherein said method further comprises culturing of a cell line via the steps of: growing a plurality of HaCat cells in an incubator at a temperature of 37°C and 5% CO2, with an RPM1 1640 medium complemented with 10 % foetal bovine serum and a penicillin and streptomycin solution; and, changing a growth medium after every three days till a monolayer of said plurality of HaCat cells is formed, wherein said plurality of HaCat cells (ATCC, Manassas, VA, USA) are human keratinocyte cells.

3. The method as claimed in Claim 1 , wherein said method further comprises preparation of BlueLight defence powder dilution via the steps of: preparing said BlueLight Defence powder dilution by dissolving it in a RPMI medium; and, preparing different dilutions preparations at the concentrations of 100ug/ml BLD, 200ug/ml BLD, 500ug/ml BLD and 1000ug/ml BLD.

4. The method as claimed in Claim 1 , wherein said plurality of ingredients comprises Rosmarinus Officinalis (Rosemary) leaves, Stigma maydis (Cornsilk), Punica granatum (pomegranate) fruit, Cucumis melo var. cantalupensis (Cantaloupe) fruit, and, Camellia sinensis (White Tea leaf).

5. The method as claimed in Claim 1 , wherein said plurality of ingredients comprises 41 .80% by weight (209 mg) of Rosmarinus Officinalis (Rosemary) leaves, 9.40% by weight (47 mg) of Stigma maydis (Cornsilk), 19.60% by weight (98 mg) of Punica granatum (pomegranate) fruit, 10.40% by weight (52 mg) of Cucumis melo var. cantalupensis (Cantaloupe) fruit, and, 18.80% by weight (94 mg) of Camellia sinensis (White Tea leaf).

6. A composition for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation, said composition comprising, Rosmarinus Officinalis (Rosemary) leaves, Stigma maydis (Cornsilk), Punica granatum (pomegranate) fruit, Cucumis melo var. cantalupensis (Cantaloupe) fruit, and, Camellia sinensis (White Tea leaf).

7. A composition for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation, said composition comprising, 41.80% by 11 weight (209 mg) of Rosmarinus Officinalis (Rosemary) leaves, 9.40% by weight (47 mg) of Stigma maydis (Cornsilk), 19.60% by weight (98 mg) of Punica granatum (pomegranate) fruit, 10.40% by weight (52 mg) of Cucumis melo var. cantalupensis (Cantaloupe) fruit, and, 18.80% by weight (94 mg) of Camellia sinensis (White Tea leaf).

Description:
COMPOSITIONS AND METHODS FOR RECOVERING FROM BLUE-LIGHT (BL) INDUCED PIGMENTATION, OXIDATIVE STRESS AND INFLAMMATION

FIELD OF THE INVENTION

Embodiments of the present invention generally relate to compositions for recovering from pathological conditions, and, more particularly, to compositions and methods for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation.

BACKGROUND OF THE INVENTION

Generally, the cytokines that regulate pro-inflammatory cytokines are a series of immunoregulatory molecules. To regulate human immune responses, cytokines perform their action using certain inhibitors of cytokine and soluble cytokine receptors. Their physiological role in inflammation and pathology is recognised more and more under systemic inflammatory conditions (Heijmans- Antonissen et al. 2006). Major cytokines, including interleukin (IL)-1 receptor antagonist, interleukin-4 (IL-4), interleukin-10 (IL-10), interleukin=11 (IL-11 ) and interleukin-13 (IL-13) can act as anti-inflammatory agents. Leukaemia inhibitory factor, interferon-alpha, interleukin-6 (IL-6), and transforming growth factor (TGF-P) are graded in different circumstances as either anti-inflammatory or pro-inflammatory cytokines. There exist multiple prior art literature for studying key anti-inflammatory cytokines and their unique human disease functions. Subsequently, such inhibitory cytokines have already shown to be successful in different clinical conditions with severe inflammation. It is also well known that IL-10 is a cytokine with good anti-inflammatory properties that suppress inflammatory cytokine expression including TNF-a, IL- 6 and IL-1 by activated macrophages. Furthermore, TGF- family antagonises the development of nitric oxide in macrophages (Roberts and Sporn 1993). Nitric oxide was heavily involved in the final typical course of neuropathic pain (Milligan et al. 2005). TGF- 1 or agents that induce its activity are expected to provide successful treatment for neuropathic pain due to its anti-cytokine action (Uceyler et al. 2006, Wieseler-Frank et al. 2004).

Moreover, other related prior art literature as disclosed in U.S. patent application 2002/0086070A1 of Kuhrts titled, “ANTI-INFLAMMATORY AND CONNECTIVE TISSUE REPAIR FORMULATIONS” and U.S. Pat. No. 6,391 ,346 titled, “ANTI-INFLAMMATORY, SLEEP-PROMOTING HERBAL COMPOSITION AND METHOD OF USE” describes an orally administered composition capable of reducing inflammation in animals. However, prior art disclosure are associated with one or more disadvantages, such as, for example, but not limited to, differences in assay methodology having profound effects on the results obtained.

Those of ordinary skills in the art will further appreciate that it is also continuously noticeable for substances that can introduce harmful factors, such as toxins, pathogens and radiations. The skin defends the body from outside environments. More recent research has focused on the influence of these dynamics, especially on the harmful effects of cigarette smoke or light exposure (Rittie and Fisher 2015). Exposure to sunlight is one of the key factors that lead to skin ageing. Ultraviolet (UV) rays including UVA (320-400 nm) and UVB (290-320 nm) have been shown to oenetrate the cutaneous barrier and can enter the skin layers of dermal and subcutaneous as with UVA and infrared radiation (IR) A (770-1400 nm). Skin damage caused by sunlight is due to the ultraviolet (UV) light and blue-violet light factor. Reports show that, over time, exposure to the blue end of the light spectrum may cause significant long-term harm to eyes and skin health problems (eyestrain, headaches, physical and mental fatigue, sleep disorders, etc.) (Austin et al. 2018, Marshall et al. 2006, Moon et al. 2008). Tyrosinase is an enzyme that contains copper that can catalyze the production of melanin and other tyrosine pigments by oxidation in plant and animal tissues. It is located within melanosomes which are synthesised in the skin melanocytes. Due to the major role of tyrosinase in pigmentation, when tyrosinase activity was downregulated it could cause whiteness of the skin (Del Marmol and Beermann 1996, Korner and Pawelek 1982).

UVA light is widely believed to lead to skin damage and carcinogenesis through photo-sensitised development of reactive oxygen species (ROS). Still, it is not assumed that clear light is involved there (Nakashima et al. 2017). Chromophores associated with mitochondria (e.g. cytochromes and flavins), which absorb the visible spectrum in the blue region, appear to include chromophores that contribute greatly to permanent damage of the DNA (Godley et al. 2005). ROS and oxidation are the key characteristics of cellular damage. This makes antioxidant therapy an effective means of minimising the harmful effects of ROS to prevent harm caused by blue light penetration. During recent decades, health benefits of natural botanicals have become significant in terms of the prevention of their possible light radiation, called "photochemopreventive effects" (Afaq et al. 2009). Studies have shown that the effect on light-mediated skin loss is effectively minimized by natural antioxidant-rich botanicals (Afaq et al. 2009, Afaq et al. 2005).

Accordingly, there remains a need in the art for innovative, novel, efficient compositions and methods for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation.

SUMMARY OF THE INVENTION

The embodiments of the present disclosure have several features, no single one of which is solely responsible for their desirable attributes. Without limiting the scope of the present embodiments as expressed by the claims that follow, their more prominent features will now be discussed briefly. After considering this discussion, and particularly after reading the section entitled “Detailed Description”, one will understand how the features of the present embodiments provide advantages.

In one embodiment, a method for preparing a composition for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation includes the steps of, preparing a BlueLight defence powder from multiple ingredients; subjecting multiple ingredients and brown algae to freeze drying and subsequent grinding to about 80 mesh powder; dissolving the BlueLight Defence in a required amount of PBS to make a solution of 20mg/ml as a stock solution; and, filtering the stock solution through a 20um filter and subsequently diluting the stock solution to a required concentration to treat a plurality of cells. In use, multiple ingredients include Rosmarinus Officinalis (Rosemary) leaves, Stigma maydis, (Cornsilk), Punica granatum (pomegranate) fruit, Cucumis melo var. cantalupensis (Cantaloupe) fruit, and, Camellia sinensis (White Tea leaf). DETAILED DESCRIPTION

Various embodiments of the present invention are aimed at providing novel and inventive solutions focused on methods and compositions for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation. Generally, Rosemary (Rosmarinus Officinalis) is an evergreen, branched bushy shrub, about one metre long, whose stems are upright, white-blue flowers and green leaves are thin, with their borders turned backwards. Nature has proved its synthetic ability to produce carbohydrates, proteins, fats, vitamins and countless natural product secondary metabolites, starting with water, carbon dioxide and mineral elements. (Al-Sereiti et al., 1999). Corn silk extracts treatment on hyperpigmented faces have dramatically reduced skin pigmentation without allergic reactions, and corn silk has a strong potential to be used as a method to suppress pigmentation (Choi et al. 2014). Likewise, for centuries since ancient civilisations, pomegranate products have been used for medicinal uses. Inflammations, fever and bronchitis have been reported in various parts of pomegranate, including fruit peels, as well as diarrhoea, dysentery, vaginitis, urinary tract disease, malaria. Besides, growing numbers of supplements and products for pomegranates are available on the market (foods, medicinal formulae and products for cosmetics). Among the strong antioxidants in peels are ellagic tannins and ellagic acid (Li et al. 2006, Mutahar S et al. 2012, Negi and Jayaprakasha 2003).

Additionally, another plant Cucumis melo var. cantalupensis (Cantaloupe) fruit exhibits strong antioxidant potential nutraceutical use so that fat auto-oxidation could be retarded (Ibrahim and El-Masry 2016). Antioxidants scavenge ROS can be highly useful to avoid oxidative disease start and/or progression. Tea polyphenols are considered to be powerful antioxidants, especially catechin products, with a particular role in the protection against these harmful diseases (Dias 2013). Also, Brown algae possess strong anti-inflammatory and antioxidant potential. Brown algae also act as a shield to the cells from UV irradiation (Kim et al., 2013). Brown algae are an essential source of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E (Jesumani et al, 2019).

Based upon the characteristics of the above-mentioned plants, the novel and inventive embodiments of the instant invention have been developed to use these plants powder as a cocktail known as Blue Light Defence for evaluation of their cumulative potential for reduction of pigmentation, oxidative stress and inflammation caused by blue light.

In accordance with an embodiment of the present invention, a method for preparing a composition for recovering from Blue-Light (BL) induced pigmentation, oxidative stress and inflammation includes the steps of, preparing a BlueLight defence powder from multiple ingredients; subjecting multiple ingredients and brown algae to freeze drying and subsequent grinding to about 80 mesh powder; dissolving the BlueLight Defence in a required amount of PBS to make a solution of 20mg/ml as a stock solution; and, filtering the stock solution through a 20um filter and subsequently diluting the stock solution to a required concentration to treat a plurality of cells. In use, multiple ingredients include Rosmarinus Officinalis (Rosemary) leaves, Stigma maydis, (Cornsilk), Punica granatum (pomegranate) fruit, Cucumis melo var. cantalupensis (Cantaloupe) fruit, and, Camellia sinensis (White Tea leaf).

In accordance with an embodiment of the present invention, the ingredients are present in the range of, 41.80% by weight (209 mg) of Rosmarinus Officinalis (Rosemary) leaves, 9.40% by weight (47 mg) of Stigma maydis (Cornsilk), 19.60% by weight (98 mg) of Punica granatum (pomegranate) fruit, 10.40% by weight (52 mg) of Cucumis melo var. cantalupensis (Cantaloupe) fruit, and, 18.80% by weight (94 mg) of Camellia sinensis (White Tea leaf), to form 500 mg of the composition as disclosed herein.

In accordance with an embodiment of the present invention, the method further includes culturing of a cell line via the steps of, growing a plurality of HaCat cells in an incubator at a temperature of 37°C and 5% CO2, with an RPMI 1640 medium complemented with 10 % foetal bovine serum and a penicillin and streptomycin solution; and, changing a growth medium after every three days till a monolayer of the plurality of HaCat cells is formed. In use, the HaCat cells (ATCC, Manassas, VA, USA) are human keratinocyte cells.

In accordance with an embodiment of the present invention, the method further includes preparation of BlueLight defence powder dilution via the steps of, preparing the BlueLight Defence powder dilution by dissolving it in a RPMI medium; and, preparing different dilutions preparations at the concentrations of 100ug/ml BLD, 200ug/ml BLD, 500ug/ml BLD and 1000ug/ml BLD.

The conditional language used herein, such as, among others, “can,” “could,” “might,” “may,” “e.g.,” and the like, unless specifically stated otherwise, or otherwise understood within the context as used, is generally intended to convey that certain embodiments include, while other embodiments do not include, certain features, elements and/or steps. Thus, such conditional language is not generally intended to imply that features, elements and/or steps are in any way required for one or more embodiments or that one or more embodiments necessarily include logic for deciding, with or without author input or prompting, whether these features, elements and/or steps are included or are to be performed in any particular embodiment. The terms "comprising," "including," 'having," and the like are synonymous and are used inclusively, in an open-ended fashion, and do not exclude additional elements, features, acts, operations, and so forth. Also, the term "or" is used in its inclusive sense (and not in its exclusive sense) so that when used, for example, to connect a list of elements, the term "or" means one, some, or all of the elements in the list.

While there has been shown and described the preferred embodiment of the instant invention it is to be appreciated that the invention may be embodied otherwise than is herein specifically shown and described and that, within said embodiment, certain changes may be made in the form and arrangement of the parts without departing from the underlying ideas or principles of this invention as outlined in the Claims appended herewith. Therefore, the appended claims are to be construed to cover all equivalents falling within the true scope and spirit of the invention.