Title:
COMPOSITIONS AND METHODS FOR SYNTHESIS OF TERPENOIDS
Document Type and Number:
WIPO Patent Application WO/2020/264400
Kind Code:
A3
Abstract:
The disclosure relates to the biosynthesis of terpenoids, such as, for example, geraniol and derivatives thereof, using genetic engineering. In particular, the disclosure relates to the biosynthesis of nepetalactol, nepetalactone, dihydronepetalactone, and derivatives thereof. The disclosure provides recombinant cells genetically engineered to produce high levels of nepetalactol, nepetalactone and/or dihydronepetalactone. The disclosure also provides methods of producing nepetalactol, nepetalactone and dihydronepetalactone using cell-based systems as well as cell-free systems.
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Inventors:
DE KOK STEFAN (US)
LAU WARREN (US)
MCSORLEY FERN (US)
MEYER HERMANN-JOSEF (US)
SERBER ZACH (US)
WAWRZYN GRAYSON (US)
LAU WARREN (US)
MCSORLEY FERN (US)
MEYER HERMANN-JOSEF (US)
SERBER ZACH (US)
WAWRZYN GRAYSON (US)
Application Number:
PCT/US2020/039959
Publication Date:
April 15, 2021
Filing Date:
June 26, 2020
Export Citation:
Assignee:
ZYMERGEN INC (US)
International Classes:
C12P17/06; C12N1/38; C12N15/52; C12R1/00
Domestic Patent References:
| WO2015002528A1 | 2015-01-08 | |||
| WO2011011412A2 | 2011-01-27 | |||
| WO2019126778A1 | 2019-06-27 |
Foreign References:
| EP2021486B1 | 2014-10-29 | |||
| US20180282744A1 | 2018-10-04 |
Other References:
CAMPBELL, ALEX: "Engineering of a nepetalactol-producing platform strain of Saccharomyces cerevisiae for the production of plant seco-iridoids", A THESIS IN THE DEPARTMENT OF BIOLOGY, September 2016 (2016-09-01), Montreal, Quebec, Canada, pages 1 - 66, XP055817314
BILLINGSLEY, JM ET AL.: "Production of semi-biosynthetic nepetalactone in yeast", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 46, no. 9-10, 5 June 2019 (2019-06-05), pages 1365 - 1370, XP036905551, DOI: 10.1007/s10295-019-02199-x
ANONYMOUS: "G418 Culture and Plate Preparation Protocol For Bacteria, Plant and Mammalian Cells, and Yeast", GOLD BIOTECHNOLOGY PROTOCOL. TD-P REVISION 3.0, 3 December 2018 (2018-12-03), pages 1 - 3, XP055817317
"Synthetic Defined (SD) Medium", COLD SPRING HARBOR PROTOCOLS, 2015, DOI: 10.1101/pdb.rec085639
LICHMAN, BR ET AL.: "Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis", NATURE CHEMICAL BIOLOGY, vol. 15, no. 1, 1 January 2019 (2019-01-01), pages 71 - 79, XP055598857, DOI: 10.1038/S41589-018-0185-2
DATABASE PROTEIN [online] NCBI; 21 April 2005 (2005-04-21), "protein S [Catharanthus roseus]", XP055817313, Database accession no. AAU95203.1
BILLINGSLEY, JM ET AL.: "Production of semi-biosynthetic nepetalactone in yeast", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 46, no. 9-10, 5 June 2019 (2019-06-05), pages 1365 - 1370, XP036905551, DOI: 10.1007/s10295-019-02199-x
ANONYMOUS: "G418 Culture and Plate Preparation Protocol For Bacteria, Plant and Mammalian Cells, and Yeast", GOLD BIOTECHNOLOGY PROTOCOL. TD-P REVISION 3.0, 3 December 2018 (2018-12-03), pages 1 - 3, XP055817317
"Synthetic Defined (SD) Medium", COLD SPRING HARBOR PROTOCOLS, 2015, DOI: 10.1101/pdb.rec085639
LICHMAN, BR ET AL.: "Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis", NATURE CHEMICAL BIOLOGY, vol. 15, no. 1, 1 January 2019 (2019-01-01), pages 71 - 79, XP055598857, DOI: 10.1038/S41589-018-0185-2
DATABASE PROTEIN [online] NCBI; 21 April 2005 (2005-04-21), "protein S [Catharanthus roseus]", XP055817313, Database accession no. AAU95203.1
Attorney, Agent or Firm:
HOLLY, David C. et al. (US)
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