Title:
CONSTANT-TEMPERATURE AMPLIMER FOR NUCLEIC ACID AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/034746
Kind Code:
A1
Abstract:
Disclosed are a constant-temperature amplimer for nucleic acids and the use thereof. The amplimer comprises a forward primer and a reverse primer used for amplifying a target gene. The forward primer is a sequence having an exogenous self-paired hairpin structure at a 5' terminal, or a sequence having, at the 5' terminal, a hairpin structure formed by matching with any source sequence located in the target gene and close to a forward primer terminal. The present invention has the effects as follows: the amplimer of the present invention is used for producing the sequence tandem repeat structure of the target gene by means of nucleic acid amplification technology, and a repetition frequency can be precisely controlled; the technology also can be used in three-generation sequencing technology; and amplification steps are simple and fast, and the accuracy of sequencing is improved.
Inventors:
LU XINHUA (CN)
Application Number:
PCT/CN2019/091694
Publication Date:
February 20, 2020
Filing Date:
June 18, 2019
Export Citation:
Assignee:
LU XINHUA (CN)
International Classes:
C12Q1/6876; C12N15/11; C12Q1/6844
Foreign References:
| CN108998509A | 2018-12-14 | |||
| CN107904284A | 2018-04-13 | |||
| CN103255227A | 2013-08-21 | |||
| CN108359716A | 2018-08-03 |
Attorney, Agent or Firm:
NANJING SUKE PATENT AGENT CO., LTD. (CN)
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