Title:
CONSTRUCTION METHOD FOR AND APPLICATION OF ENGINEERED ESCHERICHIA COLI STRAIN FIXING CO2 AND PRODUCING MALIC ACID
Document Type and Number:
WIPO Patent Application WO/2021/120237
Kind Code:
A1
Abstract:
Provided are a construction method for and an application of an engineered strain of Escherichia coli that fixes CO2 and produces malic acid, which belong to the field of fermentation. The engineered bacteria is bacteria obtained by performing genetic engineering modification on Escherichia coli MG1655. Said genetic engineering modification comprises knocking out a fumaric acid reductase gene, a fumarase gene, a lactate dehydrogenase gene and an alcohol dehydrogenase gene, and overexpressing formate dehydrogenase, acetyl-CoA synthase, acylated acetaldehyde dehydrogenase, formaldehyde lyase, dihydroxyacetone kinase, malic enzyme and phosphorous oxidoreductase so as to obtain a strain GH0407. The described strain is used for the fermentation production of malic acid. By using CO2 and glucose as co-substrates, anaerobic fermentation is carried out for 72 hours. The output of malic acid can reach 39g/L, and the yield is 1.53 mol/mol, whereas the original starting strain does not accumulate malic acid.
Inventors:
LIU LIMING (CN)
HU GUIPENG (CN)
CHEN XIULAI (CN)
MA DANLEI (CN)
LIU JIA (CN)
LUO QIULING (CN)
HU GUIPENG (CN)
CHEN XIULAI (CN)
MA DANLEI (CN)
LIU JIA (CN)
LUO QIULING (CN)
Application Number:
PCT/CN2019/127756
Publication Date:
June 24, 2021
Filing Date:
December 24, 2019
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N15/09; C12N1/20; C12N15/70; C12P7/46; C12R1/19
Domestic Patent References:
| WO2012031079A2 | 2012-03-08 |
Foreign References:
| CN104046577A | 2014-09-17 | |||
| CN106434772A | 2017-02-22 | |||
| CN104694449A | 2015-06-10 | |||
| CN101255405A | 2008-09-03 | |||
| CN1246155A | 2000-03-01 |
Other References:
DONG XIAOXIANG: "Design And Construction of L-Malate Overproducing Escherichia coli Strains", CHINESE MASTER'S THESES FULL-TEXT DATABASE, 1 June 2016 (2016-06-01), pages 1 - 48, XP055822144
WU YABIN: "The Construction and Fermentation of Recombinant Escherichia Coli for L-Malic Acid Production", CHINESE MASTER'S THESES FULL-TEXT DATABASE, 1 May 2013 (2013-05-01), pages 1 - 47, XP055822151
X. ZHANG, WANG X., SHANMUGAM K. T., INGRAM L. O.: "L-malate production by metabolically engineered Escherichia coli.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 2, 15 January 2011 (2011-01-15), pages 427 - 434, XP055441156, ISSN: 0099-2240, DOI: 10.1128/AEM.01971-10
WU YABIN: "The Construction and Fermentation of Recombinant Escherichia Coli for L-Malic Acid Production", CHINESE MASTER'S THESES FULL-TEXT DATABASE, 1 May 2013 (2013-05-01), pages 1 - 47, XP055822151
X. ZHANG, WANG X., SHANMUGAM K. T., INGRAM L. O.: "L-malate production by metabolically engineered Escherichia coli.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 2, 15 January 2011 (2011-01-15), pages 427 - 434, XP055441156, ISSN: 0099-2240, DOI: 10.1128/AEM.01971-10
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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