Title:
CULTURE MEDIUM FOR SELECTIVELY SEPARATING LACTIC ACID BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2021/005813
Kind Code:
A1
Abstract:
Heretofore, for the separation and detection of lactic acid bacteria, a BCP-supplemented plate count agar culture medium, an MRS agar culture medium, an APT agar culture medium and the like have been used. These culture media have poor selectivity for lactic acid bacteria. Therefore, when the culture media are used in specimens not containing bacteria other than lactic acid bacteria in the testing on the number of cells of lactic acid bacteria in a fermented food, any particular problem does not occur. However, when the culture media are used in specimens also containing bacteria other than lactic acid bacteria, the bacteria other than lactic acid bacteria also grow along with the lactic acid bacteria, and it is sometimes impossible to distinguish between the lactic acid bacteria and the bacteria other than the lactic acid bacteria. In this case, the accurate detection of the lactic acid bacteria may become difficult and the number of cells of the lactic acid bacteria may not be counted accurately. In these situations, the present invention provides a culture medium for selectively separating a lactic acid bacterium, which can be prepared in a simple manner and can exclude bacteria other than lactic acid bacteria. A combination of an inorganic metal salt of an organic acid and glycine is added to an existing lactic acid bacterium culture medium such as an MRS agar culture medium. By using the culture medium, it becomes possible to culture only a lactic acid bacterium selectively while excluding bacteria other than the lactic acid bacterium.
Inventors:
TERAMURA HAJIME (JP)
OGURA AYA (JP)
FUJIWARA AOI (JP)
OGURA AYA (JP)
FUJIWARA AOI (JP)
Application Number:
PCT/JP2019/050396
Publication Date:
January 14, 2021
Filing Date:
December 23, 2019
Export Citation:
Assignee:
JNC CORP (JP)
International Classes:
C12N1/20; C12Q1/04
Foreign References:
| JPH099955A | 1997-01-14 | |||
| JPH0928361A | 1997-02-04 | |||
| JP2006081536A | 2006-03-30 | |||
| JPS63219371A | 1988-09-13 | |||
| JPH0223864A | 1990-01-26 | |||
| JP2003079363A | 2003-03-18 | |||
| JP2009044983A | 2009-03-05 | |||
| JP2008193987A | 2008-08-28 |
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