CYTOCHROME P450 MUTANT PROTEIN AND USE THEREOF - CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES

Title:

CYTOCHROME P450 MUTANT PROTEIN AND USE THEREOF

Document Type and Number:

WIPO Patent Application WO/2022/105729

Kind Code:

A1

Abstract:

Provided are a cytochrome P450 (CYP716A53v2) mutant protein and the use thereof. The mutation is mutated at a site selected from the group consisting of F167V, T451A, I117S and L208C or a combination thereof on the basis of a wild-type CYP716A53v2 protein. The enzyme catalytic activity of the CYP716A53v2 mutant obtained after mutation is improved, and the yield of panaxatriol can be improved.

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Inventors:

ZHOU ZHIHUA (CN)
WANG PINGPING (CN)
YAN XING (CN)

Application Number:

PCT/CN2021/130830

Publication Date:

May 27, 2022

Filing Date:

November 16, 2021

Export Citation:

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Assignee:

CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES (CN)

International Classes:

C12N9/02

Foreign References:

CN108330111A	2018-07-27
CN109136203A	2019-01-04
KR20130137443A	2013-12-17
CN106459987A	2017-02-22
US20170121750A1	2017-05-04

Other References:

J.-Y. HAN, H.-S. HWANG, S.-W. CHOI, H.-J. KIM, Y.-E. CHOI: "Cytochrome P450 CYP716A53v2 Catalyzes the Formation of Protopanaxatriol from Protopanaxadiol During Ginsenoside Biosynthesis in Panax Ginseng", PLANT AND CELL PHSIOLOGY, OXFORD UNIVERSITY PRESS, UK, vol. 53, no. 9, 1 September 2012 (2012-09-01), UK , pages 1535 - 1545, XP055268295, ISSN: 0032-0781, DOI: 10.1093/pcp/pcs106
DATABASE Protein GenBank; ANONYMOUS : "cytochrome P450 CYP716A53v2 [Panax ginseng] - Protein - NCBI", XP055932336, retrieved from NCBI

Attorney, Agent or Firm:

SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)

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