Title:
DIGITAL PCR METHOD, CHIP, PREPARATION METHOD AND CIRCULATION SYSTEM
Document Type and Number:
WIPO Patent Application WO/2021/082951
Kind Code:
A1
Abstract:
Provided in the present invention are a digital PCR method, a chip, a preparation method and a circulation system, the method comprising: placing a PCR chip which has undergone sample introduction into a reaction chamber; evacuating air from the reaction chamber; performing pressurization of the reaction chamber; putting the pressurized reaction chamber in a reaction device and performing a PCR reaction; performing fluorescence signal analysis of the digital PCR chip.
Inventors:
XU TIEGANG (CN)
LI XINXIN (CN)
WU LEI (CN)
WANG XUEFENG (CN)
LI XINXIN (CN)
WU LEI (CN)
WANG XUEFENG (CN)
Application Number:
PCT/CN2020/121493
Publication Date:
May 06, 2021
Filing Date:
October 16, 2020
Export Citation:
Assignee:
SHANGHAI INSTITUTE OF MICROSYSTEMS AND INFORMATION TECH CHINESE ACADEMY OF SCIENCES (CN)
International Classes:
C12Q1/6851; C12M1/00; C12M1/34; C12M1/36; C12M1/38
Domestic Patent References:
WO2001023093A1 | 2001-04-05 |
Foreign References:
CN102277294A | 2011-12-14 | |||
CN103071548A | 2013-05-01 | |||
CN103777662A | 2014-05-07 | |||
CN110819698A | 2020-02-21 | |||
CN110804650A | 2020-02-18 |
Other References:
ZHU QIANGYUAN: "Self-priming Compartmentalization Digital PCR Chip for Single Nucleic Acid and Protein Molecule Detection", CHINESE DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, 1 November 2013 (2013-11-01), pages 1 - 188, XP055808621
Attorney, Agent or Firm:
SCIHEAD IP LAW FIRM (CN)
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