Title:
DNA LIBRARY CONSTRUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2021/052310
Kind Code:
A1
Abstract:
Provided is a DNA library construction method. The method comprises the following steps: (1) performing dephosphorylation on a 5' tail end of a DNA fragment; (2) providing a joint mixture, wherein the joint mixture comprises a flat-end joint and joints having different lengths of adhesive ends; (3) enabling the 5' tail ends of the joints in the joint mixture to be linked to a 3' tail end of the dephosphorylated DNA fragment, and (4) enabling the 3' tail ends of the joints in the joint mixture to be linked to the 5' tail end of the DNA fragment by means of a notch translation method.
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Inventors:
LI JIANLI (US)
ZHANG JINGLAN (CN)
ZHANG ZHIWEI (CN)
ZHANG JINGLAN (CN)
ZHANG ZHIWEI (CN)
Application Number:
PCT/CN2020/115275
Publication Date:
March 25, 2021
Filing Date:
September 15, 2020
Export Citation:
Assignee:
BEIJING BIOBIGGEN TECH CO LTD (CN)
International Classes:
C12Q1/68; C40B50/06
Foreign References:
CN110565174A | 2019-12-13 | |||
CN106459968A | 2017-02-22 | |||
CN108166067A | 2018-06-15 | |||
CN105525357A | 2016-04-27 | |||
CN108166068A | 2018-06-15 | |||
US9023769B2 | 2015-05-05 |
Attorney, Agent or Firm:
BEIJING GLOBAL LAW OFFICE (CN)
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