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Patent Searching and Data


Title:
DNA SEQUENCING METHOD
Document Type and Number:
WIPO Patent Application WO/2018/041274
Kind Code:
A3
Abstract:
Disclosed is a DNA sequencing method. The DNA sequencing method provided in the present invention comprises the following steps in order: (1) adding a tag sequence at the 3' terminus of the DNA to be sequenced so as to form a DNA to be sequenced including the tag sequence, the nucleotide sequence of said tag sequence being the reverse complement of the nucleotide sequence of the sequencing primer; (2) mixing the DNA to be sequenced including the tag sequence and the sequencing primer so as to form a product having a 5' terminus double strand and a single main strand; (3) after step (2) is completed, the product is mixed separately with dATP, dCTP, dTTP, and dGTP to obtain four systems, each system is separately added to a single-molecule device modified by a DNA polymerase, and electric signals are read. Experiments verify that the method provided in the present invention performs DNA sequencing and has important application value.

Inventors:
GUO XUEFENG (CN)
WANG XIAOLONG (CN)
CI HAINA (CN)
Application Number:
PCT/CN2017/108325
Publication Date:
April 26, 2018
Filing Date:
October 30, 2017
Export Citation:
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Assignee:
UNIV BEIJING (CN)
International Classes:
C12Q1/68
Domestic Patent References:
WO2012005857A12012-01-12
Foreign References:
CN105092647A2015-11-25
CN106244712A2016-12-21
CN104630358A2015-05-20
Other References:
DATABASE Protein [O] 14 May 2013 (2013-05-14), "DNA polymerase I [Escherichia coli]", XP055478373, Database accession no. WP_000250009.1
See also references of EP 3508587A4
Attorney, Agent or Firm:
PATENTSINO IP FIRM (CN)
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