DOUBLE ENDED SEQUENCING

Title:

DOUBLE ENDED SEQUENCING

Document Type and Number:

WIPO Patent Application WO2004070005

Kind Code:

A3

Abstract:

This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.

Inventors:

CHEN YI-JU (US)
LEAMON JOHN H (US)
LOHMAN KENTON (US)
RONAN MICHAEL T (US)
SRINIVASAN MAITHREYAN (US)
ROTHBERG JONATHAN (US)
WEINER MICHAEL (US)

Application Number:

PCT/US2004/002485

Publication Date:

November 11, 2004

Filing Date:

January 28, 2004

Export Citation:

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Assignee:

454 CORP (US)
CHEN YI-JU (US)
LEAMON JOHN H (US)
LOHMAN KENTON (US)
RONAN MICHAEL T (US)
SRINIVASAN MAITHREYAN (US)
ROTHBERG JONATHAN (US)
WEINER MICHAEL (US)

International Classes:

B01L3/00; C07H21/00; C12N15/10; C12P19/34; G01N21/25; G01N21/64; (IPC1-7): C12Q1/68; C12P19/34

Foreign References:

US5436143A	1995-07-25
US5512439A	1996-04-30
US4994372A	1991-02-19

Other References:

SANGER ET AL: "DNA sequencing with chain-terminating inhibitors", PNAS, vol. 74, no. 12, December 1977 (1977-12-01), pages 5463 - 5467, XP000603873

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