Title:
DRUG FOR ANTAGONIZING REPLICATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/068533
Kind Code:
A1
Abstract:
A drug for antagonizing the replication of porcine reproductive and respiratory syndrome virus (PRRSV) and an application thereof. By using a viral infection test, a drug which antagonizes PRRSV - xanthohumol - is discovered for the first time from among a natural drug library of 386 plant sources. Xanthohumol can effectively inhibit PRRSV replication on both Marc-145 and PAM cells. Five xanthohumol derivatives having different molecular structures are artificially synthesized, and it is discovered that a derivative Xn-4 has the strongest inhibitory effect on virus replication in vitro. Test results of artificial infection and drug therapy in piglets show that the Xn-4 can effectively inhibit PRRSV viremia, relieve clinical symptoms of infected pigs, and significantly reduce lung inflammation and pathological damage.
Inventors:
JIANG PING (CN)
LIU XUEWEI (CN)
BAI JUAN (CN)
ZHANG JUNREN (CN)
WANG XIANWEI (CN)
LI YUFENG (CN)
LIU XUEWEI (CN)
BAI JUAN (CN)
ZHANG JUNREN (CN)
WANG XIANWEI (CN)
LI YUFENG (CN)
Application Number:
PCT/CN2020/094179
Publication Date:
April 15, 2021
Filing Date:
June 03, 2020
Export Citation:
Assignee:
UNIV NANJING AGRICULTURAL (CN)
International Classes:
A61P11/00; A61K31/12; A61P31/14; C07C45/74; C07C49/84
Foreign References:
CN110613706A | 2019-12-27 | |||
KR20150049695A | 2015-05-08 | |||
CN108524476A | 2018-09-14 |
Other References:
LIU XUEWEI; BAI JUAN; JIANG CHENLONG; SONG ZHONGBAO; ZHAO YONGXIANG; NAUWYNCK HANS; JIANG PING: "Therapeutic effect of Xanthohumol against highly pathogenic porcine reproductive and respiratory syndrome viruses", VETERINARY MICROBIOLOGY, ELSEVIER BV, NL, vol. 238, 26 September 2019 (2019-09-26), NL, XP085876340, ISSN: 0378-1135, DOI: 10.1016/j.vetmic.2019.108431
ZHANG FANGJIE, LI YULIN: "Synthesis of 4′--Hydroxy--5, 7--dimethoxy--3′, 8--diallylflavanone", JOURNAL OF LANZHOU UNIVERSITY, SCIENCE EDITION, vol. 27, no. 4, 1 January 1991 (1991-01-01), pages 91 - 93, XP055798749, DOI: 10.13885/j.issn.0455-2059.1991.04.016
SEKIZAKI HARUO, YOKOSAWA RYOZO: "Studies on Zoospore-Attracting Activity.I.Synthesis of Isoflavones and Their Attracting Activity to Aphanomyces euteiches Zoospore", CHEM. PHARM. BULL, vol. 36, 1 January 1988 (1988-01-01), pages 4876 - 4880, XP055798741
LI RONG, HOU YAN-JUN, ZHOU QIANG, SUN ZHI-ZHONG: "Total synthesis of Genkwanin", JOURNAL OF NATURAL SCIENCE OF HEILONGJIANG UNIVERSITY, vol. 27, no. 4, 1 August 2010 (2010-08-01), pages 504 - 511, XP055798752, DOI: 10.13482/j.issn1001-7011.2010.04.022
CHOI DAHEUI, PARK JIN CHAN, LEE HA NA, MOON JI-HOI, AHN HYO-WON, PARK KWANGYONG, HONG JINKEE: "In Vitro Osteogenic Differentiation and Antibacterial Potentials of Chalcone Derivatives", MOLECULAR PHARMACEUTICS, AMERICAN CHEMICAL SOCIETY, US, vol. 15, no. 8, 6 August 2018 (2018-08-06), US, pages 3197 - 3204, XP055798744, ISSN: 1543-8384, DOI: 10.1021/acs.molpharmaceut.8b00288
ZHANG FANGJIE, LI YULIN: "Synthesis of 4′--Hydroxy--5, 7--dimethoxy--3′, 8--diallylflavanone", JOURNAL OF LANZHOU UNIVERSITY, SCIENCE EDITION, vol. 27, no. 4, 1 January 1991 (1991-01-01), pages 91 - 93, XP055798749, DOI: 10.13885/j.issn.0455-2059.1991.04.016
SEKIZAKI HARUO, YOKOSAWA RYOZO: "Studies on Zoospore-Attracting Activity.I.Synthesis of Isoflavones and Their Attracting Activity to Aphanomyces euteiches Zoospore", CHEM. PHARM. BULL, vol. 36, 1 January 1988 (1988-01-01), pages 4876 - 4880, XP055798741
LI RONG, HOU YAN-JUN, ZHOU QIANG, SUN ZHI-ZHONG: "Total synthesis of Genkwanin", JOURNAL OF NATURAL SCIENCE OF HEILONGJIANG UNIVERSITY, vol. 27, no. 4, 1 August 2010 (2010-08-01), pages 504 - 511, XP055798752, DOI: 10.13482/j.issn1001-7011.2010.04.022
CHOI DAHEUI, PARK JIN CHAN, LEE HA NA, MOON JI-HOI, AHN HYO-WON, PARK KWANGYONG, HONG JINKEE: "In Vitro Osteogenic Differentiation and Antibacterial Potentials of Chalcone Derivatives", MOLECULAR PHARMACEUTICS, AMERICAN CHEMICAL SOCIETY, US, vol. 15, no. 8, 6 August 2018 (2018-08-06), US, pages 3197 - 3204, XP055798744, ISSN: 1543-8384, DOI: 10.1021/acs.molpharmaceut.8b00288
Attorney, Agent or Firm:
NANJING TIANHUA PATENT AGENT CO., LTD. (CN)
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