Title:
ENGINEERED BIOSYNTHESIS OF NOVEL POLYENES
Document Type and Number:
WIPO Patent Application WO2002097082
Kind Code:
A3
Abstract:
The complete DNA sequence of the gene cluster encoding the polypeptides responsible for the biosynthesis of the polyene antibiotic amphotericin of S. nodosus is provided. Engineered alteration of the amphC) gene so as to eliminate production of amphotericin A in favour of the more active form amphotericin B, as well as the production of amphotericin analogues having altered characteristics, is enabled by manipulation of the sequences of the cluster.
Inventors:
CAFFREY JOHN PATRICK (IE)
Application Number:
PCT/IE2002/000071
Publication Date:
February 12, 2004
Filing Date:
May 27, 2002
Export Citation:
Assignee:
UNIV DUBLIN (IE)
CAFFREY JOHN PATRICK (IE)
CAFFREY JOHN PATRICK (IE)
International Classes:
C07H17/08; C07K14/36; C12N9/02; C12N9/10; C12N9/88; C12N15/11; C12N15/52; C12P17/18; C12P19/62; (IPC1-7): C12N15/11
Other References:
BRAUTASET T ET AL: "BIOSYNTHESIS OF THE POLYENE ANTIFUNGAL ANTIBIOTIC NYSTATIN IN STREPTOMYCES NOURSEI ATCC 11455: ANALYSIS OF THE GENE CLUSTER AND DEDUCTION OF THE BIOSYNTHETIC PATHWAY", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 7, no. 6, 2000, pages 395 - 403, XP000953274, ISSN: 1074-5521
ASZALOS A ET AL: "Physico-chemical and microbiological comparison of nystatin, amphotericin A and amphotericin B, and structure of amphotericin A.", THE JOURNAL OF ANTIBIOTICS. JAPAN DEC 1985, vol. 38, no. 12, December 1985 (1985-12-01), pages 1699 - 1713, XP009007494, ISSN: 0021-8820
ASZALOS A ET AL: "Physico-chemical and microbiological comparison of nystatin, amphotericin A and amphotericin B, and structure of amphotericin A.", THE JOURNAL OF ANTIBIOTICS. JAPAN DEC 1985, vol. 38, no. 12, December 1985 (1985-12-01), pages 1699 - 1713, XP009007494, ISSN: 0021-8820
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