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Title:
ENHANCED GENE EXPRESSION
Document Type and Number:
WIPO Patent Application WO/2012/071580
Kind Code:
A3
Abstract:
The present disclosure is directed to a novel, unexpected approach of expressing exogenous gene(s) at increased levels by predictably, optionally irreversibly, incorporating the gene(s) into a region of increased gene expression (RIDGE) on a chromosome of a host cell. This approach is accomplished by identification of RIDGE(s). and further by integration of integrase-specific sites (e.g., attP or attB) in the presence of or mediated by integrase. The approach renders a high level of gene expression; predictability of the location of the exogenous genes, elimination of genetic instability or unwanted phenotype; and reduction of time and cost in optimizing protein production in host cells, which will be every useful in the production of therapeutic, prophylactic, and diagnostic proteins.

Inventors:
ZHU JAMES (US)
YU GUOLIANG (US)
Application Number:
PCT/US2011/062163
Publication Date:
January 17, 2013
Filing Date:
November 25, 2011
Export Citation:
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Assignee:
APPLIED STEMCELL INC (US)
ZHU JAMES (US)
YU GUOLIANG (US)
International Classes:
C12N15/85; C12N9/00; C12N15/67
Domestic Patent References:
WO2007137267A22007-11-29
WO2009068645A12009-06-04
Foreign References:
US20100075401A12010-03-25
US20040214329A12004-10-28
Other References:
AMY C. GROTH ET AL.: "Phage integrases: biology and applications", JOURNAL OF MOLECULAR BIOLOGY, vol. 335, no. 3, 16 January 2004 (2004-01-16), pages 667 - 678, XP004480538, DOI: doi:10.1016/j.jmb.2003.09.082
HUIB CARON ET AL.: "The human transcriptome map: clustering of highly expressed genes in chromosomal domains", SCIENCE, vol. 291, no. 5507, 16 February 2001 (2001-02-16), pages 1289 - 1292, XP007901724, DOI: doi:10.1126/science.1056794
PENG JIAO ET AL.: "Retargeting of pre-set regions on chromosome for high gene expression in mammalian cells", MOLECULAR ENGINEERING OF BIOLOGICAL AND CHEMICAL SYSTEMS, DSPACE@MIT' S OPEN ACCESS ARTICLES COLLECTION, 2005, Retrieved from the Internet
Attorney, Agent or Firm:
ZHU, James (2275 East Bayshore Road Suite 101Palo Alto, California, US)
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