Title:
ENZYME CAPABLE OF DEHYDROXYLATING HYDROXYL GROUP IN UROLITHIN COMPOUND
Document Type and Number:
WIPO Patent Application WO/2021/015267
Kind Code:
A1
Abstract:
The present disclosure addresses the problem of providing an enzyme capable of dehydroxylating a hydroxyl group located at a specific position in a urolithin compound that has a hydroxyl group at at least the specific position. The problem can be solved by an enzyme having the following properties (1) and (2): (1) the enzyme can dehydroxylate a hydroxyl group located at position-4 in a urolithin compound; and (2) the enzyme can be activated by one or more components selected from the group consisting of reduced-type nicotinamide adenine dinucleotide (NADH), reduced-type nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD) and flavin adenine mononucleotide (FMN) in the presence of methyl viologen (MV).
Inventors:
OGAWA JUN (JP)
KISHINO SHIGENOBU (JP)
YAMAMOTO HIROAKI (JP)
KISHINO SHIGENOBU (JP)
YAMAMOTO HIROAKI (JP)
Application Number:
PCT/JP2020/028541
Publication Date:
January 28, 2021
Filing Date:
July 22, 2020
Export Citation:
Assignee:
DAICEL CORP (JP)
International Classes:
C12P17/06; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12N9/02; C12N15/31; C12N15/52; C12N15/53; C12N15/63
Domestic Patent References:
| WO2018124135A1 | 2018-07-05 | |||
| WO2019130681A1 | 2019-07-04 | |||
| WO2014147280A1 | 2014-09-25 |
Foreign References:
| JP2019126309A | 2019-08-01 | |||
| JPH06253861A | 1994-09-13 | |||
| JPH05176776A | 1993-07-20 |
Other References:
DATABASE GenBank [online] 13 October 2017 (2017-10-13), "Gordonibacter urolithinfaciens strain DSM 27213T genome assembly, chromosome: I", XP055786597, Database accession no. LT 900217. 1
DATABASE NCBI [online] 17 April 2017 (2017-04-17), "Gordonibacter pamelaeae 7-10-1-b draft genome", XP055786599, Database accession no. NC_021021.1
DATABASE Protein [online] 16 November 2018 (2018-11-16), "MFS transporter [Gordonibacter urolithinfaciens", XP055786603, retrieved from ncbi Database accession no. ROT88164.1
DATABASE GenBank [online] 27 February 2015 (2015-02-27), "pamelaeae 7-10-1-b", XP055786604, retrieved from ncbi Database accession no. CBL04587.1
DATABASE GenBank [online] 16 November 2018 (2018-11-16), XP055786605, Database accession no. ROT88165.1
DATABASE GenBank [online] "barrel fold [Gordonibacter pamelaeae 7-10-1-b", Database accession no. CBL04588.1
WATANABE, H. ET AL.: "Evaluation of electron- transferring cofactor mediating enzyme systems involved in urolithin dehydroxylation in Gordonibacter urolithinfaciens DSM 27213", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 129, no. 5, 23 January 2020 (2020-01-23), pages 552 - 557, XP086114859, DOI: 10.1016/j.jbiosc.2019.11.014
J. AGRIC. FOOD CHEM., vol. 56, 2008, pages 393 - 400
APPL. ENVIRON. MICROBIOL., vol. 50, 1985, pages 540 - 542
NUCLEIC ACIDS RESEARCH, vol. 37, no. 1, 2009, pages e3
RES. MICROBIOL., vol. 147, 1996, pages 133 - 143
APPL. ENVIRON. MICROBIOL., vol. 78, 2012, pages 4984 - 4994
AGRIC. BIOL. CHEM., vol. 54, no. 2, 1990, pages 437 - 441
MICROBIOL. SCI., vol. 5, 1988, pages 310 - 315
ANAEROBE, vol. 41, 2016, pages 104 - 112
"Biotechnol. Bioeng.", vol. 86, 2004, HOKKAIDO SYSTEM SCIENCE CO., LTD., pages: 136 - 148
NATURE, vol. 315, 1985, pages 592 - 594
See also references of EP 4006163A4
DATABASE NCBI [online] 17 April 2017 (2017-04-17), "Gordonibacter pamelaeae 7-10-1-b draft genome", XP055786599, Database accession no. NC_021021.1
DATABASE Protein [online] 16 November 2018 (2018-11-16), "MFS transporter [Gordonibacter urolithinfaciens", XP055786603, retrieved from ncbi Database accession no. ROT88164.1
DATABASE GenBank [online] 27 February 2015 (2015-02-27), "pamelaeae 7-10-1-b", XP055786604, retrieved from ncbi Database accession no. CBL04587.1
DATABASE GenBank [online] 16 November 2018 (2018-11-16), XP055786605, Database accession no. ROT88165.1
DATABASE GenBank [online] "barrel fold [Gordonibacter pamelaeae 7-10-1-b", Database accession no. CBL04588.1
WATANABE, H. ET AL.: "Evaluation of electron- transferring cofactor mediating enzyme systems involved in urolithin dehydroxylation in Gordonibacter urolithinfaciens DSM 27213", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 129, no. 5, 23 January 2020 (2020-01-23), pages 552 - 557, XP086114859, DOI: 10.1016/j.jbiosc.2019.11.014
J. AGRIC. FOOD CHEM., vol. 56, 2008, pages 393 - 400
APPL. ENVIRON. MICROBIOL., vol. 50, 1985, pages 540 - 542
NUCLEIC ACIDS RESEARCH, vol. 37, no. 1, 2009, pages e3
RES. MICROBIOL., vol. 147, 1996, pages 133 - 143
APPL. ENVIRON. MICROBIOL., vol. 78, 2012, pages 4984 - 4994
AGRIC. BIOL. CHEM., vol. 54, no. 2, 1990, pages 437 - 441
MICROBIOL. SCI., vol. 5, 1988, pages 310 - 315
ANAEROBE, vol. 41, 2016, pages 104 - 112
"Biotechnol. Bioeng.", vol. 86, 2004, HOKKAIDO SYSTEM SCIENCE CO., LTD., pages: 136 - 148
NATURE, vol. 315, 1985, pages 592 - 594
See also references of EP 4006163A4
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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