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Title:
ENZYME-SPECIFIC FLUORESCENT COMPOUND CAPABLE OF BEING RETAINED IN CELLS
Document Type and Number:
WIPO Patent Application WO/2015/174460
Kind Code:
A1
Abstract:
Provided is an enzyme-specific fluorescent compound capable of being retained in cells, which can emit fluorescence specifically in a target cell, particularly a cell capable of expressing a reporter enzyme such as β-galactosidase, and can covalently bind to a protein in the cell to exhibit an excellent property of being retained in the cell. The fluorescent compound comprises a compound represented by formula (I') or a salt thereof. In formula (I'), A, X, Y and R1 to R9 are as described in claim 1.

Inventors:
URANO YASUTERU (JP)
KAMIYA MAKO (JP)
DOURA TOMOHIRO (JP)
Application Number:
PCT/JP2015/063789
Publication Date:
November 19, 2015
Filing Date:
May 13, 2015
Export Citation:
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Assignee:
UNIV TOKYO (JP)
International Classes:
C07H17/04; C07D311/82; C12Q1/04; C12Q1/34
Domestic Patent References:
WO2013180181A12013-12-05
WO2011087000A12011-07-21
WO2010095450A12010-08-26
WO2007100061A12007-09-07
WO2005024049A12005-03-17
Foreign References:
JP2014065673A2014-04-17
JPH01211531A1989-08-24
Other References:
MAKO KAMIYA ET AL.: "Keiko Probe no Seimitsu Sekkei ni Motozuku in vivo Jinsoku Keiko Gan Imaging", EXPERIMENTAL MEDICINE, vol. 30, no. 7, 2012, pages 1135 - 1144, XP008185652
MAKO KAMIYA ET AL.: "Rhodol no Spiro Kanka Heiko ni Motozuku beta-Galactosidase Keiko Probe no Kaihatsu", JAPANESE SOCIETY FOR PHOTOMEDICINE AND PHOTOBIOLOGY, vol. 34, 2012, pages 63, XP008185832
KAMIYA MAKO ET AL.: "beta-Galactosidase Fluorescence Probe with Improved Cellular Accumulation Based on a Spirocyclized Rhodol Scaffold", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 133, no. 33, 2011, pages 12960 - 12963, XP055236721
TAKI MASAYASU ET AL.: "A mitochondria-targeted turn-on fluorescent probe based on a rhodol platform for the detection of copper(I", ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 12, no. 27, pages 4999 - 5005, XP055236723
See also references of EP 3144315A4
Attorney, Agent or Firm:
ONO Makoto et al. (JP)
Makoto Ono (JP)
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