Title:
ESCHERICHIA COLI-BASED RECOMBINANT STRAIN AND CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/037165
Kind Code:
A1
Abstract:
Provided is a nucleotide sequence. The sequence is a sequence formed by mutation of a base at the 1049th site of a wild-type deoB gene coding sequence shown in SEQ ID NO: 1 or a promoter nucleotide sequence by mutation of a base at the -67th site at the upstream of a nucleotide sequence shown in SEQ ID NO: 13. Also provided are an expression cassette, a recombinant protein, a recombinant vector, a recombinant strain and a construction method therefor, and use thereof in preparation of L-threonine by means of fermentation. Compared with an unmutated wild-type strain, the obtained strain can produce L-threonine having higher concentration, and reduces production costs an L-threonine production strain and has good strain stability.
Inventors:
MENG GANG (CN)
WEI AIYING (CN)
JIA HUIPING (CN)
ZHAO CHUNGUANG (CN)
ZHOU XIAOQUN (CN)
MA FENGYONG (CN)
GUO XIAOWEI (CN)
TIAN BIN (CN)
SU HOUBO (CN)
YANG LIPENG (CN)
WEI AIYING (CN)
JIA HUIPING (CN)
ZHAO CHUNGUANG (CN)
ZHOU XIAOQUN (CN)
MA FENGYONG (CN)
GUO XIAOWEI (CN)
TIAN BIN (CN)
SU HOUBO (CN)
YANG LIPENG (CN)
Application Number:
PCT/CN2020/111840
Publication Date:
March 04, 2021
Filing Date:
August 27, 2020
Export Citation:
Assignee:
INNER MONGOLIA EPPEN BIOTECH CO LTD (CN)
International Classes:
C12N15/61; C12N1/21; C12N9/90; C12N15/70; C12P13/08; C12R1/19
Foreign References:
| CN110846333A | 2020-02-28 | |||
| CN110592084A | 2019-12-20 | |||
| US7229797B1 | 2007-06-12 | |||
| US7229797B1 | 2007-06-12 | |||
| EP2580341A2 | 2013-04-17 | |||
| EP2580341A2 | 2013-04-17 | |||
| CN108504613A | 2018-09-07 | |||
| CN201910927600A | 2019-09-27 | |||
| CN201910804035A | 2019-08-28 |
Other References:
DATABASE NUCLEOTIDE 11 October 2018 (2018-10-11), ANONYMOUS: "Escherichia coli str. K-12 substr. MG1655, complete genome", XP055786868, retrieved from GENBANK Database accession no. NC_000913
DATABASE PROTEIN 3 August 2011 (2011-08-03), ANONYMOUS: "phosphopentomutase [Escherichia coli XH140A]", XP055786872, retrieved from GENBANK Database accession no. EGU25861
DATABASE PROTEIN 3 August 2011 (2011-08-03), ANONYMOUS: "phosphopentomutase [Escherichia coli XH140A]", XP055786872, retrieved from GENBANK Database accession no. EGU25861
JOLOBA, ML ET AL.: "Mutations in deoB and deoC alter an extracellular signaling pathway required for activation of the gab operon in Escherichia coli", FEMS MICROBIOLOGY LETTERS, vol. 228, no. 1, 7 November 2003 (2003-11-07), XP055786893, ISSN: 0378-1097
"GenBank", Database accession no. AP009048.1
See also references of EP 3992294A4
DATABASE PROTEIN 3 August 2011 (2011-08-03), ANONYMOUS: "phosphopentomutase [Escherichia coli XH140A]", XP055786872, retrieved from GENBANK Database accession no. EGU25861
DATABASE PROTEIN 3 August 2011 (2011-08-03), ANONYMOUS: "phosphopentomutase [Escherichia coli XH140A]", XP055786872, retrieved from GENBANK Database accession no. EGU25861
JOLOBA, ML ET AL.: "Mutations in deoB and deoC alter an extracellular signaling pathway required for activation of the gab operon in Escherichia coli", FEMS MICROBIOLOGY LETTERS, vol. 228, no. 1, 7 November 2003 (2003-11-07), XP055786893, ISSN: 0378-1097
"GenBank", Database accession no. AP009048.1
See also references of EP 3992294A4
Attorney, Agent or Firm:
BEIJING ORIGINTELLIGENCE IP LAW FIRM (CN)
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