Title:
ESCHERICHIA GENUS MICROORGANISM LACKING EUTE GENE OR PUUC GENE AND METHOD FOR PRODUCING RETINOID USING SAME
Document Type and Number:
WIPO Patent Application WO/2013/019053
Kind Code:
A3
Abstract:
The present invention relates to an Escherichia genus microorganism lacking an EutE gene or a PuuC gene, and to a method for producing retinoid using same, and more specifically, to a method for producing retinoid comprising: the Escherichia genus microorganism lacking or having a diminished EutE gene, PuuC gene, which are capable of producing retinoid, or a combination thereof; and separating retinoid from a cultivated product which is obtained by cultivating the microorganism. All of these inventions are based on a confirmation of enzyme activity with respect to retinal and retinoid of EutE and PuuC proteins.
Inventors:
KIM SEON WON (KR)
JANG HUI JEONG (KR)
YOON SANG HWAL (KR)
HA BO KYUNG (KR)
JANG HUI JEONG (KR)
YOON SANG HWAL (KR)
HA BO KYUNG (KR)
Application Number:
PCT/KR2012/006075
Publication Date:
July 04, 2013
Filing Date:
July 30, 2012
Export Citation:
Assignee:
NAT UNIV GYEONGSANG IACF (KR)
KIM SEON WON (KR)
JANG HUI JEONG (KR)
YOON SANG HWAL (KR)
HA BO KYUNG (KR)
KIM SEON WON (KR)
JANG HUI JEONG (KR)
YOON SANG HWAL (KR)
HA BO KYUNG (KR)
International Classes:
C12N1/21; C12N15/31; C12N15/70; C12P1/04
Domestic Patent References:
| WO2009116566A1 | 2009-09-24 |
Foreign References:
| KR20090078113A | 2009-07-17 |
Other References:
JANG, H. J. ET AL.: "Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system.", MICROBIAL CELL FACTORIES, vol. 10, 29 July 2011 (2011-07-29), pages 59
RODRIGUEZ, G. M. ET AL.: "Isobutyraldehyde production from Escherichia coli by removing aldehyde reductase activity.", MICROBIAL CELL FACTORIES, vol. 11, 25 June 2012 (2012-06-25), pages 90
HEMMI, H. ET AL.: "Identification of genes affecting lycopene formation in Escherichia coli transformed with carotenoid biosynthetic genes: candidates for early genes in isoprenoid biosynthesis.", J. BIOCHEM., vol. 123, no. 6, June 1998 (1998-06-01), pages 1088 - 1096
RODRIGUEZ, G. M. ET AL.: "Isobutyraldehyde production from Escherichia coli by removing aldehyde reductase activity.", MICROBIAL CELL FACTORIES, vol. 11, 25 June 2012 (2012-06-25), pages 90
HEMMI, H. ET AL.: "Identification of genes affecting lycopene formation in Escherichia coli transformed with carotenoid biosynthetic genes: candidates for early genes in isoprenoid biosynthesis.", J. BIOCHEM., vol. 123, no. 6, June 1998 (1998-06-01), pages 1088 - 1096
Attorney, Agent or Firm:
LEE, Joon Ho et al. (53 Seochojungang-ro, Seocho-gu, Seoul 137-953, KR)
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Claims: