Title:
FILAMENTOUS FUNGAL MUTANTS WITH IMPROVED HOMOLOGOUS RECOMBINATION EFFICIENCY
Document Type and Number:
WIPO Patent Application WO2005095624
Kind Code:
A3
Abstract:
The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.
More Like This:
Inventors:
DEKKER PETRUS JACOBUS THEODORU (NL)
BERG VAN DEN MARCO ALEXANDER (NL)
BERG VAN DEN MARCO ALEXANDER (NL)
Application Number:
PCT/EP2005/051464
Publication Date:
February 16, 2006
Filing Date:
March 31, 2005
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
DEKKER PETRUS JACOBUS THEODORU (NL)
BERG VAN DEN MARCO ALEXANDER (NL)
DEKKER PETRUS JACOBUS THEODORU (NL)
BERG VAN DEN MARCO ALEXANDER (NL)
International Classes:
C07K14/37; C07K14/38; C07K14/385; C12N15/80; C12N15/90; C12P21/02; C12P23/00; C12P37/00; (IPC1-7): C12N15/90; C12N5/00
Domestic Patent References:
| WO2002052026A2 | 2002-07-04 | |||
| WO2000060359A2 | 2000-10-12 | |||
| WO2001068882A2 | 2001-09-20 |
Other References:
NINOMIYA YUUKO ET AL: "Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 101, no. 33, 17 August 2004 (2004-08-17), pages 12248 - 12253, XP002346025, ISSN: 0027-8424
15 March 2005 (2005-03-15), XP002346026, Retrieved from the Internet [retrieved on 20050921]
BORKOVICH KATHERINE A ET AL: "Lessons from the genome sequence of Neurospora crassa: Tracing the path from genomic blueprint to multicellular organism.", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 68, no. 1, March 2004 (2004-03-01), pages 1 - 108, XP002346027, ISSN: 1092-2172
DATABASE NCBI NIH; 12 March 2003 (2003-03-12), "Neurospora crassa strain OR74A chromosome IV cont3.499", XP002346205, Database accession no. AABX1000206
DATABASE NCBI NIH; 12 March 2003 (2003-03-12), "Neurospora crassa strain OR74A cont3.5", XP002346206, Database accession no. AABX01000750
BOULTON SIMON J ET AL: "Identification of a Saccharomyces cerevisiae Ku80 homologue: Roles in DNA double strand break rejoining and in telomeric maintenance", NUCLEIC ACIDS RESEARCH, vol. 24, no. 23, 1996, pages 4639 - 4648, XP002346028, ISSN: 0305-1048
TSUKAMOTO Y ET AL: "Hdf1, a yeast Ku-protein homologue, is involved in illegitimate recombination, but not in homologous recombination", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 24, no. 11, 1996, pages 2067 - 2072, XP002170162, ISSN: 0305-1048
NINOMIYA YUUKO ET AL: "Analysis of non-homologous recombination genes, ncku70 and ncku80, in Neurospora crassa", GENES & GENETIC SYSTEMS, vol. 78, no. 6, December 2003 (2003-12-01), & 75TH ANNUAL MEETING OF THE GENETICS SOCIETY OF JAPAN; SENDAI, JAPAN; SEPTEMBER 24-26, 2003, pages 463, XP009054253, ISSN: 1341-7568
15 March 2005 (2005-03-15), XP002346026, Retrieved from the Internet
BORKOVICH KATHERINE A ET AL: "Lessons from the genome sequence of Neurospora crassa: Tracing the path from genomic blueprint to multicellular organism.", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 68, no. 1, March 2004 (2004-03-01), pages 1 - 108, XP002346027, ISSN: 1092-2172
DATABASE NCBI NIH; 12 March 2003 (2003-03-12), "Neurospora crassa strain OR74A chromosome IV cont3.499", XP002346205, Database accession no. AABX1000206
DATABASE NCBI NIH; 12 March 2003 (2003-03-12), "Neurospora crassa strain OR74A cont3.5", XP002346206, Database accession no. AABX01000750
BOULTON SIMON J ET AL: "Identification of a Saccharomyces cerevisiae Ku80 homologue: Roles in DNA double strand break rejoining and in telomeric maintenance", NUCLEIC ACIDS RESEARCH, vol. 24, no. 23, 1996, pages 4639 - 4648, XP002346028, ISSN: 0305-1048
TSUKAMOTO Y ET AL: "Hdf1, a yeast Ku-protein homologue, is involved in illegitimate recombination, but not in homologous recombination", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 24, no. 11, 1996, pages 2067 - 2072, XP002170162, ISSN: 0305-1048
NINOMIYA YUUKO ET AL: "Analysis of non-homologous recombination genes, ncku70 and ncku80, in Neurospora crassa", GENES & GENETIC SYSTEMS, vol. 78, no. 6, December 2003 (2003-12-01), & 75TH ANNUAL MEETING OF THE GENETICS SOCIETY OF JAPAN; SENDAI, JAPAN; SEPTEMBER 24-26, 2003, pages 463, XP009054253, ISSN: 1341-7568
Download PDF: