Title:
FLUORESCENT SOLVATOCHROMIC PIGMENT
Document Type and Number:
WIPO Patent Application WO/2010/090265
Kind Code:
A1
Abstract:
Provided is a novel fluorescent solvatochromic pigment that (1) has ionic terminals readily enabling use on a hydrophilic surface or in a polar solvent; (2) can be efficiently excited by an ordinary argon laser (488 nm); (3) shifts emission wavelengths with changes in polarity; and (4) can effectively stain a biological tissue, such as a cell. When pyridinium groups were introduced to the electron-withdrawing groups of a neutral fluorescent solvatochromic pigment (JP 2008-291210 A) to synthesize a fluorescent solvatochromic pigment, it was discovered that this fluorescent solvatochromic pigment showed a strong fluorescent wavelength response to changes in the polarity of a hydrophilic surface and that a fluorescent solvatochromic pigment modified to become a cationic pigment can be used to stain a cell and monitor the behavior thereof.
Inventors:
YAMADA KOJI
YAMAGISHI YUTAKA
AYABE TOKIYOSHI
SON SANG-HYUN
AOYAGI MAIKO
TAIRA TOSHIO (JP)
YAMAGISHI YUTAKA
AYABE TOKIYOSHI
SON SANG-HYUN
AOYAGI MAIKO
TAIRA TOSHIO (JP)
Application Number:
PCT/JP2010/051624
Publication Date:
August 12, 2010
Filing Date:
February 04, 2010
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
YAMADA KOJI
YAMAGISHI YUTAKA
AYABE TOKIYOSHI
SON SANG-HYUN
AOYAGI MAIKO
TAIRA TOSHIO (JP)
YAMADA KOJI
YAMAGISHI YUTAKA
AYABE TOKIYOSHI
SON SANG-HYUN
AOYAGI MAIKO
TAIRA TOSHIO (JP)
International Classes:
C09B57/00; C07D409/04; C09K9/02; C09K11/06; C12N5/00; C12Q1/02
Foreign References:
| JP2008291210A | 2008-12-04 | |||
| JP2003506014A | 2003-02-18 |
Other References:
O.KARTHAUS ET AL.: "MESOSCOPIC AGGREGATION CONTROL OF ORGANIC FLUOROPHORES IN DEWETTED THIN FILMS", ORGANIC NANOPHOTONICS, 2003, pages 265 - 277, XP009159930
SERGEY F.YAN ET AL.: "SWITCHABLE FLUORESCENT AND SOLVATOCHROMIC MOLECULAR PROBES BASED ON 4-AMINO-N-METHYLPHTHALIMIDE AND A PHOTOCHROMIC DIARYLETHENE", EUR.J.ORG.CHEM, 2008, pages 2531 - 2538, XP055093685
AMELIE LANSIAUX ET AL.: "DISTRIBUTION OF FURAMIDINE ANALOGUES IN TUMOR CELLS: TARGETING OF THE NUCLEUS OR MITOCHONDRIA DEPENDING ON THE AMIDINE SUBSTITUTION", CANCER RESEARCH, vol. 62, 2002, pages 7219 - 7229, XP055093692
See also references of EP 2395055A4
PHOTOCHEMISTRY AND PHOTOBIOLOGY, vol. 66, no. 4, 1997, pages 424 - 43
BIOCHIMICA ET BIOPHYSICA ACTA, vol. 423, 1976, pages 1 - 14
SERGEY F.YAN ET AL.: "SWITCHABLE FLUORESCENT AND SOLVATOCHROMIC MOLECULAR PROBES BASED ON 4-AMINO-N-METHYLPHTHALIMIDE AND A PHOTOCHROMIC DIARYLETHENE", EUR.J.ORG.CHEM, 2008, pages 2531 - 2538, XP055093685
AMELIE LANSIAUX ET AL.: "DISTRIBUTION OF FURAMIDINE ANALOGUES IN TUMOR CELLS: TARGETING OF THE NUCLEUS OR MITOCHONDRIA DEPENDING ON THE AMIDINE SUBSTITUTION", CANCER RESEARCH, vol. 62, 2002, pages 7219 - 7229, XP055093692
See also references of EP 2395055A4
PHOTOCHEMISTRY AND PHOTOBIOLOGY, vol. 66, no. 4, 1997, pages 424 - 43
BIOCHIMICA ET BIOPHYSICA ACTA, vol. 423, 1976, pages 1 - 14
Attorney, Agent or Firm:
SHIMODA Akira et al. (JP)
Shimoda Showa (JP)
Shimoda Showa (JP)
Download PDF: