FUSED PYRIMIDINE COMPOUNDS AS INHIBITORS OF MENIN-MLL INTERACTION

CROSS-REFERENCE

[0001] The present application claims the benefit of U.S. provisional application no. 63/126,505, filed December 16, 2020, the content of which is hereby incorporated by reference in its entirety.

FIELD

[0002] Described herein are compounds, methods of making such compounds, pharmaceutical compositions, and medicaments containing such compounds, and methods of using such compounds and compositions to inhibit the activity of menin-MLL.

BACKGROUND

[0003] The Histone-lysine N-methyltransferase 2 (KMT2) family of proteins, which currently consists of at least 5 members, methylate lysine 4 on the histone H3 tails at important regulatory regions in the genome and thereby impart crucial functions through the modulation of chromatin structures and DNA accessibility (Morera, Lubbert, and Jung., Clin. Epigenetics 8, 57- (2016)). These enzymes are known to play an important role in the regulation of gene expression during early development and hematopoiesis (Rao & Dou, Nat.Rev. Cancer 15, 334-346 (2015)).

[0004] The human KMT2 family was initially named the mixed-lineage leukaemia (MLL) family, owing to the role of the first-found member in this disease, KMT2A which is still commonly referred to as MLL1 or MLL in routine clinical practice.

[0005] KMT2A (MLL1) is frequently found to be cytogenetically targeted in several types of leukemia (e.g., ALL and AML), and in those cases where balanced chromosomal translocations are found, these typically target KMT2A (MLL1) and one of over 80 translocation partner genes that have been described to date (Winters and Bemt, Front. Pediatr. 5, 4 (2017)). These chromosomal anomalies often result in the formation of fusion genes that encode fusion proteins which are believed to be causally related to the onset and/or progression of the disease. Inhibition of menin may be a promising strategy for treating MLL related diseases, including leukemia.

[0006] M-525 is a highly potent, irreversible small molecule inhibitor of the menin-MLL proteinprotein interaction. It forms a covalent bond with Cys329 residue in menin. M-525 demonstrate high cellular specificity over non-MLL leukemia cells and is >30 times more potent that the corresponding reversible inhibitors. See S. Xu et al. Angewandte Chemie International Ed. 57(6), 1601-1605 (2017).

SUMMARY

[0007] Described herein are inhibitors of menin-MLL interaction. Also described herein are specific heterocyclic inhibitors of menin-MLL or MLL fusion proteins interaction. In some embodiments, the inhibitors of menin-MLL interaction are irreversible inhibitors. In some embodiments, the inhibitors of menin-MLL interaction are reversible inhibitors.

[0008] Also, described herein are irreversible inhibitors of menin-MLL interaction. Also described herein are specific heterocyclic irreversible inhibitors of menin-MLL or MLL fusion proteins interaction.

[0009] Also described herein are methods for synthesizing such irreversible inhibitors, methods for using such irreversible inhibitors in the treatment of diseases (including diseases wherein inhibition of menin-MLL interaction provides therapeutic benefit to a patient having the disease). Further described are pharmaceutical compositions that include an inhibitor of menin-MLL interaction. Specifically, described herein are compounds and methods of use thereof to inhibit interaction of menin with MLL oncoproteins (e.g., MLL1, MLL2, MLL-fusion oncoproteins).

[0010] Specifically described herein are irreversible inhibitors of menin-MLL interaction that form a covalent bond with a cysteine residue on menin. Further described herein are irreversible inhibitors of menin-MLL interaction that form a covalent bond with a Cys329 residue on menin. Also described are pharmaceutical formulations that include an irreversible inhibitor of menin.

[0011] Thus, in some embodiments, provided herein are methods for preventing, treating or ameliorating in a mammal a disease or condition that is causally related to the aberrant activity of a menin-MLL interaction in vivo, which comprises administering to the mammal an effective diseasetreating or condition-treating amount of a compound according to Formula (L-I) having the structure:

Cy ¹ — Cy ² -X-W-Y-Cy ³ — L — Cy ⁴ — R ¹

(L-I) or a pharmaceutically acceptable salt thereof, wherein:

Cy ¹ is substituted or unsubstituted

Cy ² is substituted or unsubstituted

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(R ^3b ) ₂ -, -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b )2-, or -O-; or X-W-Y is -N(H)-, or -S(O) ₂ -N(H)C(R ^3b ) ₂ -;

Cy ³ is substituted or unsubstituted phenylene, pyridylene, pyrimidinylene, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

L is a single bond, substituted or unsubstituted -N(H)-, -C(F)2-O-, or substituted or unsubstituted Ci-4 alkylene; Cy ⁴ is absent, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

R ¹ is -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-C(O)-C(O)- C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-C(O)-C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -O-C(R ^8a ) ₂ - C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), - N(R ^3c )-S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), -S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), each R ^8a is independently H or Ci-4 alkyl; each R ^3a , R ^3b , and R ^3c is independently H or substituted or unsubstituted Ci-4 alkyl; each R ^6a and R ^6b is independently H, CN, halo, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond;

R ^6C is H, CN, halo, or Ci-6 alkyl, unsubstituted or substituted with substituted or unsubstituted amino, or substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each R ^6e and R ^6f is independently H, CN, halo, or Ci-6 alkyl;

R ⁷ is H, an optionally substituted group selected from alkyl, a 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, phenyl, an 8-10 membered bicyclic aryl ring, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; and provided that: i. when Cy ¹ is substituted or unsubstituted -X-W-Y- is -NH-C(O)-; Cy ³ is substituted or unsubstituted phenyl, or substituted or unsubstituted pyrid-2-yl; R ^3c is H; L is -CH2-; Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene; R ¹ is N(H)-C(O)-CR ^6a =CR ^6b R ^6c ; each R ^6a and R ^6b is independently H, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; and R ^6c is Ci-6 alkyl, substituted with substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur; then Cy ² is other than substituted or unsubstituted phenylene; ii. when Cy ¹ is

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b )2-, or -O-;

Cy ³ is substituted or unsubstituted phenylene, or substituted or unsubstituted pyridylene, L is a single bond or -CH2-; and Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene; R ¹ is N(H)-C(O)-CR ^6a =CR ^6b R ^6c ; each R ^6a and R ^6b is independently H, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; and R ^6c is H, or substituted or unsubstituted Ci-6 alkyl; then Cy ² is other than substituted or unsubstituted phenylene, or R ⁷ is other than morpholinyl; or iii) the compound is:

[0012] In certain embodiments, when R ^6a and R ^6b are joined together to form a bond, they form, or otherwise indicate, a triple bond between the adjacent atoms.

[0013] In some embodiments, the target site is a cavity in which the compound or the moiety binds to the MLL site on the menin. In some embodiments, the active site is MEN1 at the MLL binding site.

[0014] In some embodiments, the the disease or condition is an autoimmune disease, a heteroimmune disease, a cancer, mastocytosis, osteoporosis or bone resorption disorder, or an inflammatory disease.

[0015] In some embodiments, the compounds provided herein may also serve as an anti-tumor agents through off-target activity by impacting other protein-protein interactions as well as kinases. [0016] In some embodiments, provided herein are pharmaceutical compositions comprising a therapeutically effective amount of a compound of Formula (I) and a pharmaceutically acceptable excipient. In some embodiments, the pharmaceutical composition comprising the compound of Formula (I) is formulated for a route of administration selected from oral administration, parenteral administration, buccal administration, nasal administration, topical administration, or rectal administration. In some embodiments, provided herein are methods for treating an autoimmune disease or condition comprising administering to a patient in need a therapeutically effective amount of a compound of Formula (I). In some embodiments the autoimmune disease is selected from rheumatoid arthritis or lupus. In some embodiments, provided herein is a method for treating a heteroimmune disease or condition comprising administering to a patient in need a therapeutically effective amount of a compound of Formula (I). In some embodiments provided herein is a method for treating a cancer comprising administering to a patient in need a therapeutically effective amount of a compound of Formula (I). In some embodiments, the cancer is a myeloid line of blood cells. In some embodiments, the cancer is a lymphoid line of blood cell. In some embodiments, the cancer is a B-cell proliferative disorder. In some embodiments, the cancer is a lymphoid line of blood cells. [0017] In some embodiments the myeloid line of blood cells is acute myeloid leukemia. In some embodiments the lymphoid line of blood cells is acute lymphoblastic leukemia. In some embodiments the B-cell proliferative disorder is diffuse large B cell lymphoma, follicular lymphoma or chronic lymphocytic leukemia. In some embodiments the cancer (soft tissue) is glioblastoma and pancreatic cancer. In some embodiments the cancer is renal cell carcinoma.

[0018] In some embodiments, provided herein is a method for treating mastocytosis comprising administering to a patient in need a therapeutically effective amount of a compound of Formula (I). [0019] In some embodiments, provided herein is a method for treating osteoporosis or bone resorption disorders comprising administering to a patient in need a therapeutically effective amount of a compound of Formula (I).

[0020] In some embodiments, provided herein is a method for treating an inflammatory disease or condition comprising administering to a patient in need a therapeutically effective amount of a compound of Formula (I).

[0021] Any combination of the groups described above for the various variables is contemplated herein. It is understood that substituents and substitution patterns on the compounds provided herein can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be synthesized by techniques known in the art, as well as those set forth herein.

[0022] In some embodiments, provided herein are pharmaceutical compositions, which include a therapeutically effective amount of at least one of any of the compounds herein, or a pharmaceutically acceptable salt, pharmaceutically active metabolite, pharmaceutically acceptable prodrug, or pharmaceutically acceptable solvate. In certain embodiments, compositions provided herein further include a pharmaceutically acceptable diluent, excipient and/or binder.

[0023] Pharmaceutical compositions formulated for administration by an appropriate route and means containing effective concentrations of one or more of the compounds provided herein, or pharmaceutically effective derivatives thereof, that deliver amounts effective for the treatment, prevention, or amelioration of one or more symptoms of dieases, disorders or conditions that are modulated or otherwise affected by Menin-MLL activity, or in which Menin-MLL activity is implicated, are provided. The effective amounts and concentrations are effective for ameliorating any of the symptoms of any of the diseases, disorders or conditions disclosed herein. [0024] In certain embodiments, provided herein is a pharmaceutical composition containing: i) a physiologically acceptable carrier, diluent, and/or excipient; and ii) one or more compounds provided herein.

[0025] In some embodiments, provided herein are methods for treating a patient by administering a compound provided herein. In some embodiments, provided herein is a method of inhibiting the activity of Menin-MLL, or of treating a disease, disorder, or condition, which would benefit from inhibition of Menin-MLL activity, in a patient, which includes administering to the patient a therapeutically effective amount of at least one of any of the compounds herein, or pharmaceutically acceptable salt, pharmaceutically active metabolite, pharmaceutically acceptable prodrug, or pharmaceutically acceptable solvate.

[0026] In some embodiments, provided herein is the use of a compound disclosed herein for inhibiting Menin-MLL activity or for the treatment of a disease, disorder, or condition, which would benefit from inhibition of Menin-MLL activity.

[0027] In some embodiments, compounds provided herein are administered to a human.

[0028] In some embodiments, compounds provided herein are orally administered.

[0029] In some embodiments, compounds provided herein are used for the formulation of a medicament for the inhibition of Menin-MLL activity. In some embodiments, compounds provided herein are used for the formulation of a medicament for the inhibition of Menin-MLL activity.

[0030] Articles of manufacture including packaging material, a compound or composition or pharmaceutically acceptable derivative thereof provided herein, which is effective for inhibiting the activity of Menin-MLL, within the packaging material, and a label that indicates that the compound or composition, or pharmaceutically acceptable salt, pharmaceutically active metabolite, pharmaceutically acceptable prodrug, or pharmaceutically acceptable solvate thereof, is used for inhibiting the activity of Menin-MLL, are provided.

[0031] In some embodiments, provided herein is a method for inhibiting Menin-MLL activity in a subject in need thereof by administering to the subject thereof a composition containing a therapeutically effective amount of at least one compound having the structure of Formula (I). In some embodiments, the subject in need is suffering from an autoimmune disease, e.g., inflammatory bowel disease, arthritis, lupus, rheumatoid arthritis, psoriatic arthritis, osteoarthritis, Still’s disease, juvenile arthritis, diabetes, myasthenia gravis, Hashimoto’s thyroiditis, Ord’s thyroiditis, Graves’ disease Sjogren’s syndrome, multiple sclerosis, Guillain-Barre syndrome, acute disseminated encephalomyelitis, Addison’s disease, opsoclonus-myoclonus syndrome, ankylosing spondylitis, antiphospholipid antibody syndrome, aplastic anemia, autoimmune hepatitis, coeliac disease, Goodpasture’s syndrome, idiopathic thrombocytopenic purpura, optic neuritis, scleroderma, primary biliary cirrhosis, Reiter’s syndrome, Takayasu’s arteritis, temporal arteritis, warm autoimmune hemolytic anemia, Wegener’s granulomatosis, psoriasis, alopecia universalis, Behget’s disease, chronic fatigue, dysautonomia, endometriosis, interstitial cystitis, neuromyotonia, scleroderma, or vulvodynia.

[0032] In some embodiments, the subject in need is suffering from a heteroimmune condition or disease, e.g., graft versus host disease, transplantation, transfusion, anaphylaxis, allergy, type I hypersensitivity, allergic conjunctivitis, allergic rhinitis, or atopic dermatitis.

[0033] In certain embodiments, the subject in need is suffering from an inflammatory disease, e.g., asthma, appendicitis, blepharitis, bronchiolitis, bronchitis, bursitis, cervicitis, cholangitis, cholecystitis, colitis, conjunctivitis, cystitis, dacryoadenitis, dermatitis, dermatomyositis, encephalitis, endocarditis, endometritis, enteritis, enterocolitis, epicondylitis, epididymitis, fasciitis, fibrositis, gastritis, gastroenteritis, hepatitis, hidradenitis suppurativa, laryngitis, mastitis, meningitis, myelitis myocarditis, myositis, nephritis, oophoritis, orchitis, osteitis, otitis, pancreatitis, parotitis, pericarditis, peritonitis, pharyngitis, pleuritis, phlebitis, pneumonitis, pneumonia, proctitis, prostatitis, pyelonephritis, rhinitis, salpingitis, sinusitis, stomatitis, synovitis, tendonitis, tonsillitis, uveitis, vaginitis, vasculitis, or vulvitis.

[0034] In some embodiments, the subject in need is suffering from a cancer. In some embodiments, the cancer is a B-cell proliferative disorder, e.g., diffuse large B cell lymphoma, follicular lymphoma, chronic lymphocytic lymphoma, chronic lymphocytic leukemia, B-cell prolymphocytic leukemia, lymphoplasmacytic lymphoma/Waldenstrbm macroglobulinemia, splenic marginal zone lymphoma, plasma cell myeloma, plasmacytoma, extranodal marginal zone B cell lymphoma, nodal marginal zone B cell lymphoma, mantle cell lymphoma, mediastinal (thymic) large B cell lymphoma, intravascular large B cell lymphoma, primary effusion lymphoma, burkitt lymphoma/leukemia, or lymphomatoid granulomatosis. In some embodiments, where the subject is suffering from a cancer, an anti-cancer agent is administered to the subject in addition to one of the above-mentioned compounds.

[0035] In some embodiments, the subject in need is suffering from a thromboembolic disorder, e.g., myocardial infarct, angina pectoris, reocclusion after angioplasty, restenosis after angioplasty, reocclusion after aortocoronary bypass, restenosis after aortocoronary bypass, stroke, transitory ischemia, a peripheral arterial occlusive disorder, pulmonary embolism, or deep venous thrombosis. [0036] In some embodiments, provided herein is a method for treating an autoimmune disease by administering to a subject in need thereof a composition containing a therapeutically effective amount of at least one compound having the structure of Formula (L-I), (L-II), and (I). In some embodiments, the autoimmune disease is arthritis. In some embodiments, the autoimmune disease is lupus. In some embodiments, the autoimmune disease is inflammatory bowel disease (including Crohn’s disease and ulcerative colitis), rheumatoid arthritis, psoriatic arthritis, osteoarthritis, Still’s diseasejuvenile arthritis, lupus, diabetes, myasthenia gravis, Hashimoto’s thyroiditis, Ord’s thyroiditis, Graves’ disease Sjogren’s syndrome, multiple sclerosis, Guillain-Barre syndrome, acute disseminated encephalomyelitis, Addison’s disease, opsoclonus-myoclonus syndrome, ankylosing spondylitis, antiphospholipid antibody syndrome, aplastic anemia, autoimmune hepatitis, coeliac disease, Goodpasture’s syndrome, idiopathic thrombocytopenic purpura, optic neuritis, scleroderma, primary biliary cirrhosis, Reiter’s syndrome, Takayasu’s arteritis, temporal arteritis, warm autoimmune hemolytic anemia, Wegener’s granulomatosis, psoriasis, alopecia universalis, Behget’s disease, chronic fatigue, dysautonomia, endometriosis, interstitial cystitis, neuromyotonia, scleroderma, or vulvodynia.

[0037] In some embodiments, provided herein is a method for treating a heteroimmune condition or disease by administering to a subject in need thereof a composition containing a therapeutically effective amount of at least one compound having the structure Formula (L-I), (L-II), and (I). In some embodiments, the heteroimmune condition or disease is graft versus host disease, transplantation, transfusion, anaphylaxis, allergy, type I hypersensitivity, allergic conjunctivitis, allergic rhinitis, or atopic dermatitis.

[0038] In some embodiments, provided herein is a method for treating an inflammatory disease by administering to a subject in need thereof a composition containing a therapeutically effective amount of at least one compound having the structure of Formula (L-I), (L-II), and (I). In some embodiments, the inflammatory disease is asthma, inflammatory bowel disease (including Crohn’s disease and ulcerative colitis), appendicitis, blepharitis, bronchiolitis, bronchitis, bursitis, cervicitis, cholangitis, cholecystitis, colitis, conjunctivitis, cystitis, dacryoadenitis, dermatitis, dermatomyositis, encephalitis, endocarditis, endometritis, enteritis, enterocolitis, epicondylitis, epididymitis, fasciitis, fibrositis, gastritis, gastroenteritis, hepatitis, hidradenitis suppurativa, laryngitis, mastitis, meningitis, myelitis myocarditis, myositis, nephritis, oophoritis, orchitis, osteitis, otitis, pancreatitis, parotitis, pericarditis, peritonitis, pharyngitis, pleuritis, phlebitis, pneumonitis, pneumonia, proctitis, prostatitis, pyelonephritis, rhinitis, salpingitis, sinusitis, stomatitis, synovitis, tendonitis, tonsillitis, uveitis, vaginitis, vasculitis, or vulvitis.

[0039] In some embodiments, provided herein is a method for treating a cancer by administering to a subject in need thereof a composition containing a therapeutically effective amount of at least one compound having the structure of Formula (L-I), (L-II), and (I). In some embodiments, the cancer is a B-cell proliferative disorder, e.g., diffuse large B cell lymphoma, follicular lymphoma, chronic lymphocytic lymphoma, chronic lymphocytic leukemia, B-cell prolymphocytic leukemia, lymphoplasmacytic lymphoma/Waldenstrbm macroglobulinemia, splenic marginal zone lymphoma, plasma cell myeloma, plasmacytoma, extranodal marginal zone B cell lymphoma, nodal marginal zone B cell lymphoma, mantle cell lymphoma, mediastinal (thymic) large B cell lymphoma, intravascular large B cell lymphoma, primary effusion lymphoma, burkitt lymphoma/leukemia, or lymphomatoid granulomatosis. In some embodiments, where the subject is suffering from a cancer, an anti-cancer agent is administered to the subject in addition to one of the above-mentioned compounds.

[0040] In some embodiments, provided herein is a method for treating a thromboembolic disorder by administering to a subject in need thereof a composition containing a therapeutically effective amount of at least one compound having the structure of Formula (L-I), (L-II), and (I). In some embodiments, the thromboembolic disorder is myocardial infarct, angina pectoris, reocclusion after angioplasty, restenosis after angioplasty, reocclusion after aortocoronary bypass, restenosis after aortocoronary bypass, stroke, transitory ischemia, a peripheral arterial occlusive disorder, pulmonary embolism, or deep venous thrombosis.

[0041] In some embodiments are methods for treating inflammation comprising administering to the mammal at least once an effective amount of at least one compound having the structure of Formula (L-I), (L-II), and (I).

[0042] In some embodiments, provided herein are methods for the treatment of cancer comprising administering to the mammal at least once an effective amount of at least one compound having the structure of Formula (L-I), (L-II), and (I). The type of cancer may include, but is not limited to, pancreatic cancer and other solid or hematological tumors.

[0043] In some embodiments, provided herein are methods for treating respiratory diseases comprising administering to the mammal at least once an effective amount of at least one compound having the structure Formula (L-I), (L-II), and (I). In asome embodiments, the respiratory disease is asthma. In some embodiments, the respiratory disease includes, but is not limited to, adult respiratory distress syndrome and allergic (extrinsic) asthma, non-allergic (intrinsic) asthma, acute severe asthma, chronic asthma, clinical asthma, nocturnal asthma, allergen-induced asthma, aspirinsensitive asthma, exercise-induced asthma, isocapnic hyperventilation, child-onset asthma, adultonset asthma, cough-variant asthma, occupational asthma, steroid-resistant asthma, and seasonal asthma.

[0044] In some embodiments, provided herein are methods for preventing rheumatoid arthritis and osteoarthritis comprising administering to the mammal at least once an effective amount of at least one compound having the structure of Formula (L-I), (L-II), and (I).

[0045] In some embodiments, provided herein are methods for treating inflammatory responses of the skin comprising administering to the mammal at least once an effective amount of at least one compound having the structure of Formula (L-I), (L-II), and (I). Such inflammatory responses of the skin include, by way of example, dermatitis, contact dermatitis, eczema, urticaria, rosacea, and scarring. In another aspect are methods for reducing psoriatic lesions in the skin, joints, or other tissues or organs, comprising administering to the mammal an effective amount of a first compound having the structure of Formula (L-I), (L-II), and (I)

[0046] In certain embodiments, provided herein are methods for treating the following diseases or conditions comprising administering to the mammal a compound provided herein. In some embodiments, the disease or condition is ALL (Acute Lymphoblastic Lymphoma), DLBCL (Diffuse Large B-Cell Lymphoma), FL (Follicular Lymphoma), RCC (Renal Cell Carcinoma), Childhoon Medulloblastoma, Glioblastoma, Pancreatic tumor or cancer, Liver cancer (Hepatocellular Carcinoma), Prostate Cancer (Myc), Triple Negative Breast (Myc), AML (Acute Myeloid Leukemia), or MDS (Myelo Dyslplastic Syndrome). In some embodiments, the disease or condition is Early-onset Dystonia. In yet some embodiments, the disease or condition is Kabuki Syndrome. [0047] In some embodiments, the disease or condition is p53 driven tumor. RUNX2 signaling pathway is one of survival signals specific to p53 defective cancer cells. RUNX2 recruits the Menin/MLLl epigenetic complex to induce the expression of MYC. Using small molecule irreversible inhibitors of the Menin/MLLl complex, targeting RUNX2/Menin/MLL1/MYC axis is a feasible strategy for killing p53 defective cancer cells (Shih, et al., A RUNX2 -Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. PLOS Genetics, https://doi.org/10.1371/journal.pgen.1005884, 2016).

[0048] In some embodiments, the disease or condition is MYC driven tumor. MYC is documented to be involved broadly in many cancers, in which its expression is estimated to be elevated or deregulated in up to 70% of human cancers. High levels of MYC expression have been linked to aggressive human prostate cancer and triple negative breast cancer (Gurel et al., Mod Pathol. 2008 Sep; 21(9): 1156-67; Palaskas et al., Cancer Res. 2011 Aug 1; 71(15):5164-74). Experimental models of Myc-mediated tumori genesis suggest that established tumors are addicted to Myc and that deregulated expression of Myc result in an addiction not only to Myc but also to nutrients. These Myc-induced changes provide a unique opportunity for new therapeutic strategies. Notwithstanding the fact that normal proliferating cells (stem cell compartments and immune cells) also use MYC for renewal, many studies have focused on targeting Myc for cancer therapeutics. Strategies have emerged to inhibit MYC expression, to interrupt Myc-Max dimerization, to inhibit Myc-Max DNA binding, and to interfere with key Myc target genes (Dang et al. Cell. 2012, 149(1): 22-35).

[0049] In any of the aforementioned embodiments are some embodiments in which administration is enteral, parenteral, or both, and wherein (a) an effective amount of a provided compound is systemically administered to the mammal; (b) an effective amount of a provided compound is administered orally to the mammal; (c) an effective amount of a provided compound is intravenously administered to the mammal; (d) an effective amount of a provided compound is administered by inhalation; (e) an effective amount of a provided compound is is administered by nasal administration; or (f) an effective amount of a provided compound is is administered by injection to the mammal; (g) an effective amount of a provided compound is is administered topically (dermal) to the mammal; (h) an effective amount of a provided compound is is administered by ophthalmic administration; or (i) an effective amount of a provided compound is is administered rectally to the mammal.

[0050] In any of the aforementioned embodiments are some embodiments comprising single administrations of an effective amount of a provided compound, including some embodiments in which (i) a provided compound is administered once; (ii) a provided compound is administered to the mammal multiple times over the span of one day; (iii) continually; or (iv) continuously.

[0051] In any of the aforementioned embodiments are some embodiments comprising multiple administrations of an effective amount of a provided compound, including some embodiments in which (i) a provided compound is administered in a single dose; (ii) the time between multiple administrations is every 6 hours; (iii) a provided compound is administered to the mammal every 8 hours. In some embodiments, the method comprises a drug holiday, wherein the administration of the compound is temporarily suspended or the dose of the compound being administered is temporarily reduced; at the end of the drug holiday, dosing of the compound is resumed. The length of the drug holiday can vary from 2 days to 1 year.

[0052] In any of the aforementioned embodiments involving the treatment of proliferative disorders, including cancer, are some embodiments comprising administering at least one additional agent selected from the group consisting of alemtuzumab, arsenic trioxide, asparaginase (pegylated or non-), bevacizumab, cetuximab, platinum-based compounds such as cisplatin, cladribine, daunorubicin/doxorubicin/idarubicin, irinotecan, fludarabine, 5 -fluorouracil, gemtuzumab, methotrexate, Paclitaxel™, taxol, temozolomide, thioguanine, or classes of drugs including hormones (an antiestrogen, an antiandrogen, or gonadotropin releasing hormone analogues, interferons such as alpha interferon, nitrogen mustards such as busulfan or melphalan or mechlorethamine, retinoids such as tretinoin, topoisomerase irreversible inhibitors such as irinotecan or topotecan, tyrosine kinase irreversible inhibitors such as gefinitinib or imatinib, or agents to treat signs or symptoms induced by such therapy including allopurinol, filgrastim, granisetron/ondansetron/palonosetron, dronabinol.

[0053] In some embodiments, the compounds of Formula (L-I), (L-II), and (I) are irreversible inhibitors of Menin-MLL activity. In certain embodiments, such irreversible inhibitors have an IC50 below 10 microM in enzyme assay. In some embodiments, a menin-MLL inhibitor has an IC50 of less than 1 microM, and in some embodiments, less than 0.25 microM.

[0054] Other objects, features, and advantages of the methods and compositions described herein will become apparent from the following detailed description. It should be understood, however, that the detailed description and the specific examples, while indicating specific embodiments, are given by way of illustration only, since various changes and modifications within the spirit and scope of the present disclosure will become apparent to those skilled in the art from this detailed description. The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described. All documents, or portions of documents, cited in the application including, but not limited to, patents, patent applications, articles, books, manuals, and treatises are hereby expressly incorporated by reference in their entirety for any purpose.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Certain Terminology

[0055] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which the claimed subject matter belongs. In the event that there are a plurality of definitions for terms herein, those in this section prevail. Where reference is made to a URL or other such identifier or address, it is understood that such identifiers can change and particular information on the internet can come and go, but equivalent information can be found by searching the internet. Reference thereto evidences the availability and public dissemination of such information.

[0056] It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of any subject matter claimed. In this application, the use of the singular includes the plural unless specifically stated otherwise. It must be noted that, as used in the specification and the appended claims, the singular forms “a,” “an” and “the” include plural referents unless the context clearly dictates otherwise. Use of the term “including” as well as other forms, such as “include”, “includes,” and “included,” is not limiting.Defmition of standard chemistry terms may be found in reference works, including Carey and Sundberg “ADVANCED ORGANIC CHEMISTRY 4 ^TH ED.” Vols. A (2000) and B (2001), Plenum Press, New York. Unless otherwise indicated, conventional methods of mass spectroscopy, NMR, HPLC, protein chemistry, biochemistry, recombinant DNA techniques and pharmacology, within the skill of the art are employed. Unless specific definitions are provided, the nomenclature employed in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those known in the art. Standard techniques can be used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients. Standard techniques can be used for recombinant DNA, oligonucleotide synthesis, and tissue culture and transformation (e.g., electroporation, lipofection). Reactions and purification techniques can be performed e.g., using kits of manufacturer’s specifications or as commonly accomplished in the art or as described herein. The foregoing techniques and procedures can be generally performed of conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification.

[0057] It is to be understood that the methods and compositions described herein are not limited to the particular methodology, protocols, cell lines, constructs, and reagents described herein and as such may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the methods and compositions described herein, which will be limited only by the appended claims. [0058] All publications and patents mentioned herein are incorporated herein by reference in their entirety for the purpose of describing and disclosing, for example, the constructs and methodologies that are described in the publications, which might be used in connection with the methods, compositions and compounds described herein. The publications discussed herein are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the inventors described herein are not entitled to antedate such disclosure by virtue of prior invention or for any other reason.

[0059] “Alkyl” refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing no unsaturation, having from one to fifteen carbon atoms (e.g., C1-C15 alkyl). In certain embodiments, an alkyl comprises one to thirteen carbon atoms (e.g., C1-C13 alkyl). In certain embodiments, an alkyl comprises one to eight carbon atoms (e.g., Ci-Cs alkyl). In some embodiments, an alkyl comprises five to fifteen carbon atoms (e.g., C5-C15 alkyl). In certain embodiments, an alkyl comprises five to eight carbon atoms (e.g., Cs-Cs alkyl). The alkyl is attached to the rest of the molecule by a single bond, for example, methyl (Me), ethyl (Et), n-propyl (n-pr), 1 -methyl ethyl (iso-propyl or i-Pr), n-butyl (n-Bu), n-pentyl, 1,1 -dimethyl ethyl (t-butyl, or t- Bu), 3 -methylhexyl, 2-methylhexyl, and the like. Unless stated otherwise specifically in the specification, an alkyl group is optionally substituted as defined and described below and herein. [0060] The alkyl group could also be a “lower alkyl” having 1 to 6 carbon atoms.

[0061] As used herein, Ci-C _x includes C1-C2, C1-C3 . . . Ci-C _x

[0062] “Alkenyl” refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one double bond, and having from two to twelve carbon atoms. In certain embodiments, an alkenyl comprises two to eight carbon atoms. In some embodiments, an alkenyl comprises two to four carbon atoms. The alkenyl is attached to the rest of the molecule by a single bond, for example, ethenyl (i.e., vinyl), prop-l-enyl (i.e., allyl), but-l-enyl, pent-l-enyl, penta- 1,4-dienyl, and the like. Unless stated otherwise specifically in the specification, an alkenyl group is optionally substituted as defined and described below and herein.

[0063] “Alkynyl” refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one triple bond, having from two to twelve carbon atoms. In certain embodiments, an alkynyl comprises two to eight carbon atoms. In some embodiments, an alkynyl has two to four carbon atoms. The alkynyl is attached to the rest of the molecule by a single bond, for example, ethynyl, propynyl, butynyl, pentynyl, hexynyl, and the like. Unless stated otherwise specifically in the specification, an alkynyl group is optionally substituted as defined and described below and herein.

[0064] “Alkylene” or “alkylene chain” refers to a straight or branched divalent hydrocarbon chain linking the rest of the molecule to a radical group, consisting solely of carbon and hydrogen, containing no unsaturation and having from one to twelve carbon atoms, for example, methylene, ethylene, propylene, n-butylene, and the like. The alkylene chain is attached to the rest of the molecule through a single bond and to the radical group through a single bond. The points of attachment of the alkylene chain to the rest of the molecule and to the radical group can be through one carbon in the alkylene chain or through any two carbons within the chain. Unless stated otherwise specifically in the specification, an alkylene chain is optionally substituted as defined and described below and herein.

[0065] “Alkenylene” or “alkenylene chain” refers to a straight or branched divalent hydrocarbon chain linking the rest of the molecule to a radical group, consisting solely of carbon and hydrogen, containing at least one double bond and having from two to twelve carbon atoms, for example, ethenylene, propenylene, n-butenylene, and the like. The alkenylene chain is attached to the rest of the molecule through a double bond or a single bond and to the radical group through a double bond or a single bond. The points of attachment of the alkenylene chain to the rest of the molecule and to the radical group can be through one carbon or any two carbons within the chain. Unless stated otherwise specifically in the specification, an alkenylene chain is optionally substituted as defined and described below and herein. “Aryl” refers to a radical derived from an aromatic monocyclic or multicyclic hydrocarbon ring system by removing a hydrogen atom from a ring carbon atom. The aromatic monocyclic or multicyclic hydrocarbon ring system contains only hydrogen and carbon from six to eighteen carbon atoms, where at least one of the rings in the ring system is fully unsaturated, i.e., it contains a cyclic, delocalized (4n+2) rr-electron system in accordance with the Hiickel theory. Aryl groups include, but are not limited to, groups such as phenyl (Ph), fluorenyl, and naphthyl. Unless stated otherwise specifically in the specification, the term “aryl” or the prefix “ar-“ (such as in “aralkyl”) is meant to include aryl radicals optionally substituted as defined and described below and herein.

[0066] “Aralkyl” refers to a radical of the formula -R ^c -aryl where R ^c is an alkylene chain as defined above, for example, benzyl, diphenylmethyl and the like. The alkylene chain part of the aralkyl radical is optionally substituted as described above for an alkylene chain. The aryl part of the aralkyl radical is optionally substituted as described above for an aryl group. [0067] “Aralkenyl” refers to a radical of the formula -R ^d -aryl where R ^d is an alkenylene chain as defined above. The aryl part of the aralkenyl radical is optionally substituted as described above for an aryl group. The alkenylene chain part of the aralkenyl radical is optionally substituted as defined above for an alkenylene group.

[0068] “Aralkynyl” refers to a radical of the formula -R ^e -aryl, where R ^e is an alkynylene chain as defined above. The aryl part of the aralkynyl radical is optionally substituted as described above for an aryl group. The alkynylene chain part of the aralkynyl radical is optionally substituted as defined above for an alkynylene chain.

[0069] “Carbocyclyl” refers to a stable non-aromatic monocyclic or polycyclic hydrocarbon radical consisting solely of carbon and hydrogen atoms, which includes fused or bridged ring systems, having from three to fifteen carbon atoms. In certain embodiments, a carbocyclyl comprises three to ten carbon atoms. In some embodiments, a carbocyclyl comprises five to seven carbon atoms. The carbocyclyl is attached to the rest of the molecule by a single bond. Carbocyclyl is optionally saturated, (i.e., containing single C-C bonds only) or unsaturated (i.e., containing one or more double bonds or triple bonds.) A fully saturated carbocyclyl radical is also referred to as “cycloalkyl.” Examples of monocyclic cycloalkyls include, e.g., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. An unsaturated carbocyclyl is also referred to as “cycloalkenyl.” Examples of monocyclic cycloalkenyls include, e.g., cyclopentenyl, cyclohexenyl, cycloheptenyl, and cyclooctenyl. Polycyclic carbocyclyl radicals include, for example, adamantyl, norbomyl (i.e., bicyclo[2.2.1]heptanyl), norbomenyl, decalinyl, 7,7-dimethyl-bicyclo[2.2.1]heptanyl, and the like. Unless otherwise stated specifically in the specification, the term “carbocyclyl” is meant to include carbocyclyl radicals that are optionally substituted as defined and described below and herein.

“Halo” or “halogen” refers to bromo, chloro, fluoro or iodo substituents.

[0070] The terms “haloalkyl,” “haloalkenyl,” “haloalkynyl” and “haloalkoxy” include alkyl, alkenyl, alkynyl and alkoxy structures in which at least one hydrogen is replaced with a halogen atom. In certain embodiments in which two or more hydrogen atoms are replaced with halogen atoms, the halogen atoms are all the same as one another. In some embodiments in which two or more hydrogen atoms are replaced with halogen atoms, the halogen atoms are not all the same as one another.

[0071] “Fluoroalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more fluoro radicals, as defined above, for example, trifluoromethyl, difluoromethyl, 2,2,2-trifluoroethyl, l-fluoromethyl-2-fluoroethyl, and the like. The alkyl part of the fluoroalkyl radical is optionally substituted as defined above for an alkyl group.

[0072] As used herein, the term “non-aromatic heterocycle”, “heterocycloalkyl” or “heteroalicyclic” refers to a non-aromatic ring wherein one or more atoms forming the ring is a heteroatom. A “non-aromatic heterocycle” or “heterocycloalkyl” group refers to a cycloalkyl group that includes at least one heteroatom selected from nitrogen, oxygen and sulfur. The radicals may be fused with an aryl or heteroaryl. Heterocycloalkyl rings can be formed by three to 14 ring atoms, such as three, four, five, six, seven, eight, nine, or more than nine atoms. A “non-aromatic heterocycle” or “heterocycloalkyl” can be saturated or unsaturated. Heterocycloalkyl rings can be optionally substituted. In certain embodiments, non-aromatic heterocycles contain one or more carbonyl or thiocarbonyl groups such as, for example, oxo- and thio-containing groups. Examples of heterocycloalkyls include, but are not limited to, lactams, lactones, cyclic imides, cyclic thioimides, cyclic carbamates, tetrahydrothiopyran, 4H-pyran, tetrahydropyran, piperidine, 1,3 -di oxin, 1,3- dioxane, 1,4-dioxin, 1,4-dioxane, piperazine, 1,3-oxathiane, 1,4-oxathiin, 1,4-oxathiane, tetrahydro- 1,4-thiazine, 2H-l,2-oxazine, mal eimide, succinimide, barbituric acid, thiobarbituric acid, dioxopiperazine, hydantoin, dihydrouracil, morpholine, trioxane, hexahydro-1, 3, 5-triazine, tetrahydrothiophene, tetrahydrofuran, pyrroline, pyrrolidine, pyrrolidone, pyrrolidione, pyrazoline, pyrazolidine, imidazoline, imidazolidine, 1,3-dioxole, 1,3 -di oxolane, 1,3-dithiole, 1,3 -di thiolane, isoxazoline, isoxazolidine, oxazoline, oxazolidine, oxazolidinone, thiazoline, thiazolidine, and 1,3- oxathiolane. Illustrative examples of heterocycloalkyl groups, also referred to as non-aromatic heterocycles, include: and the like. The term heteroalicyclic also includes all ring forms of the carbohydrates, including but not limited to the monosaccharides, the disaccharides and the oligosaccharides. Depending on the structure, a heterocycloalkyl group can be a monoradical or a diradical (i.e., a heterocycloalkylene group). The term heterocycloalkyl also includes fused, bicyclics, tricyclics, bridged, spiro and other ring forms.

[0073] “Heteroaryl” refers to a radical derived from a 3- to 18-membered aromatic ring radical that comprises two to seventeen carbon atoms and from one to six heteroatoms selected from nitrogen, oxygen and sulfur. As used herein, the heteroaryl radical is a monocyclic, bicyclic, tricyclic or tetracyclic ring system, wherein at least one of the rings in the ring system is fully unsaturated, i.e., it contains a cyclic, delocalized (4n+2) rr-electron system in accordance with the Hiickel theory. Heteroaryl includes fused or bridged ring systems. In some embodiments, heteroaryl rings have five, six, seven, eight, nine, or more than nine ring atoms. The heteroatom(s) in the heteroaryl radical is optionally oxidized. One or more nitrogen atoms, if present, are optionally quatemized. The heteroaryl is attached to the rest of the molecule through any atom of the ring(s). Examples of heteroaryls include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzindolyl, 1,3-benzodioxolyl, benzofuranyl, benzooxazolyl, benzo[d]thiazolyl, benzothiadiazolyl, benzo[b][l,4]dioxepinyl, benzo[b][l,4]oxazinyl, 1,4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranonyl, benzothienyl (benzothiophenyl), benzothieno[3,2-d]pyrimidinyl, benzotriazolyl, benzo[4,6]imidazo[l,2-a]pyridinyl, carbazolyl, cinnolinyl, cyclopenta[d]pyrimidinyl,

6.7-dihydro-5H-cyclopenta[4,5]thieno[2,3-d]pyrimidinyl, 5,6-dihydrobenzo[h]quinazolinyl, 5,6-dihydrobenzo[h]cinnolinyl, 6,7-dihydro-5H-benzo[6,7]cyclohepta[l,2-c]pyridazinyl, dibenzofuranyl, dibenzothiophenyl, furanyl, furan onyl, furo[3,2-c]pyridinyl,

5.6.7.8.9.10-hexahydrocycloocta[d]pyrimidinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyridazinyl,

5.6.7.8.9.10-hexahydrocycloocta[d]pyridinyl,isothiazolyl, imidazolyl, indazolyl, indolyl, indazolyl, isoindolyl, indolinyl, isoindolinyl, isoquinolyl, indolizinyl, isoxazolyl,

5.8-methano-5,6,7,8-tetrahydroquinazolinyl, naphthyridinyl, 1,6-naphthyri dinonyl, oxadiazolyl, 2-oxoazepinyl, oxazolyl, oxiranyl, 5,6,6a,7,8,9,10,10a-octahydrobenzo[h]quinazolinyl,

1 -phenyl- IH-pyrrolyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, pyrrolyl, pyrazolyl, pyrazolo[3,4-d]pyrimidinyl, pyridinyl, pyrido[3,2-d]pyrimidinyl, pyrido[3,4-d]pyrimidinyl, pyrazinyl, pyrimidinyl, pyridazinyl, pyrrolyl, quinazolinyl, quinoxalinyl, quinolinyl, isoquinolinyl, tetrahydroquinolinyl, 5,6,7,8-tetrahydroquinazolinyl,

5.6.7.8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidinyl,

6.7.8.9-tetrahydro-5H-cyclohepta[4,5]thieno[2,3-d]pyrimid inyl, 5,6,7,8-tetrahydropyrido[4,5-c]pyridazinyl, thiazolyl, thiadiazolyl, triazolyl, tetrazolyl, triazinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-d]pyrimidinyl, thieno[2,3-c]pridinyl, and thiophenyl (i.e. thienyl). Unless stated otherwise specifically in the specification, the term “heteroaryl” is meant to include heteroaryl radicals as defined above which are optionally substituted as defined and described below and herein.

[0074] “N-heteroaryl” refers to a heteroaryl radical as defined above containing at least one nitrogen and where the point of attachment of the heteroaryl radical to the rest of the molecule is through a nitrogen atom in the heteroaryl radical. An N-heteroaryl radical is optionally substituted as described above for heteroaryl radicals.

[0075] “C-heteroaryl” refers to a heteroaryl radical as defined above and where the point of attachment of the heteroaryl radical to the rest of the molecule is through a carbon atom in the heteroaryl radical. A C-heteroaryl radical is optionally substituted as described above for heteroaryl radicals.

[0076] “Heteroarylalkyl” refers to a radical of the formula -R ^c -heteroaryl, where R ^c is an alkylene chain as defined above. If the heteroaryl is a nitrogen-containing heteroaryl, the heteroaryl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heteroarylalkyl radical is optionally substituted as defined above for an alkylene chain. The heteroaryl part of the heteroarylalkyl radical is optionally substituted as defined above for a heteroaryl group.

[0077] “Sulfanyl” refers to the -S- radical.

[0078] “Sulfinyl” refers to the -S(=O)- radical.

[0079] “Sulfonyl” refers to the -S(=O)2- radical.

[0080] “Amino” refers to the -NH2 radical.

[0081] “Cyano” refers to the -CN radical. [0082] “Nitro” refers to the -NO2 radical. [0083] “Oxa” refers to the -O- radical. [0084] “Oxo” refers to the =0 radical. [0085] “Imino” refers to the =NH radical.

[0086] “Thioxo” refers to the =S radical.

[0087] An “alkoxy” group refers to a (alkyl)O- group, where alkyl is as defined herein. [0088] An “aryloxy” group refers to an (aryl)O- group, where aryl is as defined herein.

[0089] “Carbocyclylalkyl” means an alkyl radical, as defined herein, substituted with a carbocyclyl group. “Cycloalkylalkyl” means an alkyl radical, as defined herein, substituted with a cycloalkyl group. Non-limiting cycloalkylalkyl groups include cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl, cyclohexylmethyl, and the like.

[0090] As used herein, the terms “heteroalkyl” “heteroalkenyl” and “heteroalkynyl” include optionally substituted alkyl, alkenyl and alkynyl radicals in which one or more skeletal chain atoms is a heteroatom, e.g., oxygen, nitrogen, sulfur, silicon, boron, phosphorus or combinations thereof. The heteroatom(s) may be placed at any interior position of the heteroalkyl group or at the position at which the heteroalkyl group is attached to the remainder of the molecule. Examples include, but are not limited to, -CH2-O-CH3, -CH2-CH2-O-CH3, -CH2-NH-CH3, -CH2-CH2-NH-CH3, -CH ₂ - N(CH ₃ )-CH ₃ , -CH2-CH2-NH-CH3, -CH ₂ -CH ₂ -N(CH3)-CH3, -CH2-S-CH2-CH3, -CH ₂ -CH ₂ ,-S(O)- CH ₃ , -CH ₂ -CH ₂ -S(O)2-CH3, -CH=CH-O-CH ₃ , -Si(CH ₃ )3, -CH ₂ -CH=N-OCH ₃ , and -CH=CH- N(CH3)-CH3. In addition, up to two heteroatoms may be consecutive, such as, by way of example, - CH2-NH-OCH3 and -CH ₂ -O-Si(CH ₃ )3.

[0091] The term “heteroatom” refers to an atom other than carbon or hydrogen. Heteroatoms are typically independently selected from among oxygen, sulfur, nitrogen, silicon and phosphorus, but are not limited to these atoms. In embodiments in which two or more heteroatoms are present, the two or more heteroatoms can all be the same as one another, or some or all of the two or more heteroatoms can each be different from the others.

[0092] The term “bond,” “direct bond” or “single bond” refers to a chemical bond between two atoms, or two moi eties when the atoms joined by the bond are considered to be part of larger substructure.

[0093] An “isocyanate” group refers to a -NCO group.

[0094] An “isothiocyanate” group refers to a -NCS group.

[0095] The term “moiety” refers to a specific segment or functional group of a molecule. Chemical moieties are often recognized chemical entities embedded in or appended to a molecule.

[0096] A “thioalkoxy” or “alkylthio” group refers to a -S-alkyl group.

[0097] A “alkylthioalkyl” group refers to an alkyl group substituted with a -S-alkyl group. [0098] As used herein, the term “acyloxy” refers to a group of formula RC(=O)O-.

[0099] “Carboxy” means a -C(O)OH radical.

[00100] As used herein, the term “acetyl” refers to a group of formula -C(=O)CH3.

[00101] “Acyl” refers to the group -C(O)R.

[00102] As used herein, the term “trihalomethanesulfonyl” refers to a group of formula X3CS(=O)2- where X is a halogen.

[00103] “Cyanoalkyl” means an alkyl radical, as defined herein, substituted with at least one cyano group.

[00104] As used herein, the term “N-sulfonamido” or “sulfonylamino” refers to a group of formula RS(=O) ₂ NH-.

[00105] As used herein, the term “O-carbamyl” refers to a group of formula -OC(=O)NR2.

[00106] As used herein, the term “N-carbamyl” refers to a group of formula ROC(=O)NH-.

[00107] As used herein, the term “O-thiocarbamyl” refers to a group of formula -OC(=S)NR2. [00108] As used herein, “N-thiocarbamyl” refers to a group of formula ROC(=S)NH-.

[00109] As used herein, the term “C-amido” refers to a group of formula -C(=O)NR ₂ .

[00110] “Aminocarbonyl” refers to a -CONH2 radical.

[00111] As used herein, the term “N-amido” refers to a group of formula RC(=O)NH-.

[00112] “Hydroxyalkyl” refers to an alkyl radical, as defined herein, substituted with at least one hydroxy group. Non-limiting examples of a hydroxyalkyl include, but are not limited to, hydroxymethyl, 2-hydroxyethyl, 2-hydroxypropyl, 3 -hydroxypropyl, l-(hydroxymethyl)-

2-methylpropyl, 2-hydroxybutyl, 3 -hydroxybutyl, 4-hydroxybutyl, 2,3 -dihydroxypropyl, l-(hydroxymethyl)-2 -hydroxyethyl, 2,3 -dihydroxybutyl, 3,4-dihydroxybutyl and 2-(hydroxymethyl)-

3 -hydroxypropyl.

[00113] “Alkoxyalkyl” refers to an alkyl radical, as defined herein, substituted with an alkoxy group, as defined herein.

[00114] An “alkenyloxy” group refers to a (alkenyl)O- group, where alkenyl is as defined herein. [00115] The term “alkylamine” refers to the -N(alkyl) _x H _y group, where x and y are selected from among x=l, y=l and x=2, y=0. When x=2, the alkyl groups, taken together with the N atom to which they are attached, can optionally form a cyclic ring system.

[00116] “Alkylaminoalkyl” refers to an alkyl radical, as defined herein, substituted with an alkylamine, as defined herein. [00117] An “amide” is a chemical moiety with the formula -C(O)NHR or -NHC(O)R, where R is selected from among alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon). An amide moiety may form a linkage between an amino acid or a peptide molecule and a compound described herein, thereby forming a prodrug. Any amine, or carboxyl side chain on the compounds described herein can be amidified. The procedures and specific groups to make such amides are known to those of skill in the art and can readily be found in reference sources such as Greene and Wuts, Protective Groups in Organic Synthesis, 3 ^rd Ed., John Wiley & Sons, New York, NY, 1999, which is incorporated herein by reference in its entirety.

[00118] The term “ester” refers to a chemical moiety with formula -COOR, where R is selected from among alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon). Any hydroxy, or carboxyl side chain on the compounds described herein can be esterified. The procedures and specific groups to make such esters are known to those of skill in the art and can readily be found in reference sources such as Greene and Wuts, Protective Groups in Organic Synthesis, 3 ^rd Ed., John Wiley & Sons, New York, NY, 1999, which is incorporated herein by reference in its entirety.

[00119] As used herein, the term “ring” refers to any covalently closed structure. Rings include, for example, carbocycles (e.g., aryls and cycloalkyls), heterocycles (e.g., heteroaryls and non-aromatic heterocycles), aromatics (e.g. aryls and heteroaryls), and non-aromatics (e.g., cycloalkyls and non- aromatic heterocycles). Rings can be optionally substituted. Rings can be monocyclic or polycyclic. [00120] As used herein, the term “ring system” refers to one, or more than one ring.

[00121] The term “membered ring” can embrace any cyclic structure. The term “membered” is meant to denote the number of skeletal atoms that constitute the ring. Thus, for example, cyclohexyl, pyridine, pyran and thiopyran are 6-membered rings and cyclopentyl, pyrrole, furan, and thiophene are 5-membered rings.

[00122] The term “fused” refers to structures in which two or more rings share one or more bonds. [00123] As described herein, compounds provided herein may be “optionally substituted”. In general, the term “substituted,” whether preceded by the term “optionally” or not, means that one or more hydrogens of a designated moiety are replaced with a suitable substituent. Unless otherwise indicated, an “optionally substituted” group may have a suitable substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position. Combinations of substituents provided herein are preferably those that result in the formation of stable or chemically feasible compounds. The term “stable,” as used herein, refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and, in certain embodiments, their recovery, purification, and use for one or more of the purposes disclosed herein.

[00124] Suitable monovalent substituents on a substitutable carbon atom of an “optionally substituted” group are independently halogen; -(CH2)o-4R°; -(CH2)o-40R°; -0(CH2)o-4R°, -0-(CH2)o- 4C(O)OR°; -(CH2)O-4CH(OR°)2; -(CH2)O-4SR°; -(CH2)o-4Ph, which may be substituted with R°; - (CH2)o-40(CH2)o-iPh which may be substituted with R°; -CH=CHPh, which may be substituted with R°; -(CH2)o-40(CH2)o-i-pyridyl which may be substituted with R°; -NO2; -CN; -N ₃ ; -(CH2)o-4N(R°)2; -(CH ₂ )O-4N(R ⁰ )C(0)R°; -N(R°)C(S)R°; -(CH ₂ )O-4N(R°)C(0)NR ⁰ 2; -N(R°)C(S)NR ^O ₂ ; -(CH ₂ )O- ₄ N(R ^O )C(O)OR°; -N(R°)N(R°)C(O)R°; -N(R ^O )N(R°)C(O)NR°2; -N(R°)N(R°)C(O)OR°; -(CH ₂ )O- ₄ C(O)R°; -C(S)R°; -(CH ₂ )O-4C(0)OR°; -(CH ₂ )O-4C(0)SR°; -(CH ₂ )o-4C(0)OSiR° ₃ ; -(CH ₂ )o-40C(0)R°; -OC(0)(CH ₂ )O-4SR-, -SC(S)SR°; -(CH ₂ )O-4SC(0)R°; -(CH ₂ )O-4C(0)NR°2; -C(S)NR ^O ₂ ; - C(S)SR°; -(CH ₂ )O-40C(0)NR°2; -C(O)N(OR°)R°; -C(O)C(O)R°; -C(O)CH ₂ C(O)R ^O ; - C(NOR°)R°; -(CH ₂ )O-4SSR°; -(CH ₂ )O-4S(0)2R°; -(CH ₂ )O-4S(0)20R°; -(CH ₂ )O-40S(0)2R°; - S(O) ₂ NR°2; -(CH ₂ )O-4S(0)R°; -N(R ^O )S(O) ₂ NR°2; -N(R ^O )S(O) ₂ R°; -N(OR°)R°; -C(NH)NR ^O ₂ ; - P(O)2R°; -P(O)R ^O 2; -OP(O)R ^O 2; -OP(O)(OR ^O )2; SiR° ₃ ; -(C1-4 straight or branched alkylene)O- N(R°)2; or -(C1-4 straight or branched alkylene)C(O)O-N(R°)2, wherein each R° may be substituted as defined below and is independently hydrogen, C1-6 aliphatic, -CH2PF1, -0(CH2)o-iPh, -CH2-(5-6 membered heteroaryl ring), or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0- 4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R°, taken together with their intervening atom(s), form a 3-12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, which may be substituted as defined below.

[00125] Suitable monovalent substituents on R° (or the ring formed by taking two independent occurrences of R° together with their intervening atoms), are independently halogen, -(CH2)o-2R*, - (haloR*), -(CH ₂ )O- ₂ OH, -(CH ₂ )O-20R*, -(CH ₂ )O-2CH(OR*)2; -O(haloR’), -CN, -N ₃ , -(CH ₂ )o-2C(0)R*, -(CH ₂ )O-2C(0)OH, -(CH ₂ )O- ₂ C(0)OR*, -(CH ₂ )O- ₂ SR*, -(CH ₂ )O- ₂ SH, -(CH ₂ )O-2NH ₂ , -(CH ₂ )O-2NHR*, - (CH2)O-2NR*2, -NO2, -SiR* ₃ , -OSiR* ₃ , -C(O)SR* -(C1-4 straight or branched alkylene)C(O)OR*, or - SSR* wherein each R* is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently selected from C1-4 aliphatic, -CH ₂ Ph, -0(CH2)o-iPh, or a 5-6- membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. Suitable divalent substituents on a saturated carbon atom of R° include =0 and =S.

[00126] Suitable divalent substituents on a saturated carbon atom of an “optionally substituted” group include the following: =0, =S, =NNR* ₂ , =NNHC(O)R*, =NNHC(O)OR*, =NNHS(O) ₂ R*, =NR*, =N0R*, -O(C(R* ₂ )) ₂ -3O-, or -S(C(R* ₂ )) ₂ -3S-, wherein each independent occurrence of R* is selected from hydrogen, Ci-6 aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. Suitable divalent substituents that are bound to vicinal substitutable carbons of an “optionally substituted” group include: -O(CR* ₂ ) ₂ -3O-, wherein each independent occurrence of R* is selected from hydrogen, Ci-6 aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

[00127] Suitable substituents on the aliphatic group of R* include halogen, -R*, -(haloR*), -OH, - OR", -O(haloR’), -CN, -C(O)OH, -C(O)OR*, -NH ₂ , -NHR*, -NR* ₂ , or -NO ₂ , wherein each R* is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently Ci-4 aliphatic, -CH ₂ Ph, -0(CH ₂ )o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

[00128] Suitable substituents on a substitutable nitrogen of an “optionally substituted” group include -R', -NR' ₂ , -C(O)R ^t , -C(O)OR ^t , -C(O)C(O)R ^t , -C(O)CH ₂ C(O)R ^t , -S(O) ₂ R ^t , -SCO^NR^, - C^NR^, -C(NH)NR^ ₂ , or -N(R^)S(O) ₂ R^; wherein each R ¹ ' is independently hydrogen, Ci-6 aliphatic which may be substituted as defined below, unsubstituted -OPh, or an unsubstituted 5-6- membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R', taken together with their intervening atom(s) form an unsubstituted 3-12- membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

[00129] Suitable substituents on the aliphatic group of R' are independently halogen, -R*, -(haloR*), -OH, -OR*, -O(haloR*), -CN, -C(O)OH, -C(O)OR*, -NH ₂ , -NHR*, -NR* ₂ , or -NO ₂ , wherein each R* is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently Ci-4 aliphatic, -CFFPh, -0(CH2)o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

[00130] The term “nucleophile” or “nucleophilic” refers to an electron rich compound, or moiety thereof.

[00131] The term “electrophile”, or “electrophilic” refers to an electron poor or electron deficient molecule, or moiety thereof. Examples of electrophiles include, but in no way are limited to, Michael acceptor moieties.

[00132] The term “acceptable” or “pharmaceutically acceptable”, with respect to a formulation, composition or ingredient, as used herein, means having no persistent detrimental effect on the general health of the subject being treated or does not abrogate the biological activity or properties of the compound, and is relatively nontoxic.

[00133] As used herein, “amelioration” of the symptoms of a particular disease, disorder or condition by administration of a particular compound or pharmaceutical composition refers to any lessening of severity, delay in onset, slowing of progression, or shortening of duration, whether permanent or temporary, lasting or transient that can be attributed to or associated with administration of the compound or composition.

[00134] “Bioavailability” refers to the percentage of the weight of compounds disclosed herein, such as, compounds of any of Formula (L-I), (L-II), and (I) dosed that is delivered into the general circulation of the animal or human being studied. The total exposure (AUC(o-oo)) of a drug when administered intravenously is usually defined as 100% bioavailable (F%). “Oral bioavailability” refers to the extent to which compounds disclosed herein, such as, compounds of any of Formula (L- I), (L-II), and (I) are absorbed into the general circulation when the pharmaceutical composition is taken orally as compared to intravenous injection.

[00135] “Blood plasma concentration” refers to the concentration of compounds disclosed herein, such as, compounds of any of Formula (L-I), (L-II), and (I) in the plasma component of blood of a subject. It is understood that the plasma concentration of compounds of any of Formula (L-I), (L-II), and (I) may vary significantly between subjects, due to variability with respect to metabolism and/or possible interactions with other therapeutic agents. In accordance with some embodiments disclosed herein, the blood plasma concentration of the compounds of any of Formula (L-I), (L-II), and (I) may vary from subject to subject. Likewise, values such as maximum plasma concentration (Cmax) or time to reach maximum plasma concentration (Tmax), or total area under the plasma concentration time curve (AUC(o-oo)) may vary from subject to subject. Due to this variability, the amount necessary to constitute “a therapeutically effective amount” of a compound of any of Formula (L-I), (L-II), and (I) may vary from subject to subject.

[00136] The terms “co-administration” or the like, as used herein, are meant to encompass administration of the selected therapeutic agents to a single patient, and are intended to include treatment regimens in which the agents are administered by the same or different route of administration or at the same or different time.

[00137] The terms “effective amount” or “therapeutically effective amount,” as used herein, refer to a sufficient amount of an agent or a compound being administered which will relieve to some extent one or more of the symptoms of the disease or condition being treated. The result can be reduction and/or alleviation of the signs, symptoms, or causes of a disease, or any other desired alteration of a biological system. For example, an “effective amount” for therapeutic uses is the amount of the composition including a compound as disclosed herein required to provide a clinically significant decrease in disease symptoms without undue adverse side effects. An appropriate “effective amount” in any individual case may be determined using techniques, such as a dose escalation study. The term “therapeutically effective amount” includes, for example, a prophylactically effective amount. An “effective amount” of a compound disclosed herein is an amount effective to achieve a desired pharmacologic effect or therapeutic improvement without undue adverse side effects. It is understood that “an effect amount” or “a therapeutically effective amount” can vary from subject to subject, due to variation in metabolism of the compound of any of Formula (L-I), (L-II), and (I), age, weight, general condition of the subject, the condition being treated, the severity of the condition being treated, and the judgment of the prescribing physician. By way of example only, therapeutically effective amounts may be determined by routine experimentation, including but not limited to a dose escalation clinical trial.

[00138] The terms “enhance” or “enhancing” means to increase or prolong either in potency or duration a desired effect. By way of example, “enhancing” the effect of therapeutic agents refers to the ability to increase or prolong, either in potency or duration, the effect of therapeutic agents on during treatment of a disease, disorder or condition. An “enhancing-effective amount,” as used herein, refers to an amount adequate to enhance the effect of a therapeutic agent in the treatment of a disease, disorder or condition. When used in a patient, amounts effective for this use will depend on the severity and course of the disease, disorder or condition, previous therapy, the patient’s health status and response to the drugs, and the judgment of the treating physician.

[00139] The term “identical,” as used herein, refers to two or more sequences or subsequences which are the same. In addition, the term “substantially identical,” as used herein, refers to two or more sequences which have a percentage of sequential units which are the same when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using comparison algorithms or by manual alignment and visual inspection. By way of example only, two or more sequences may be “substantially identical” if the sequential units are about 60% identical, about 65% identical, about 70% identical, about 75% identical, about 80% identical, about 85% identical, about 90% identical, or about 95% identical over a specified region. Such percentages to describe the “percent identity” of two or more sequences. The identity of a sequence can exist over a region that is at least about 75-100 sequential units in length, over a region that is about 50 sequential units in length, or, where not specified, across the entire sequence. This definition also refers to the complement of a test sequence. By way of example only, two or more polypeptide sequences are identical when the amino acid residues are the same, while two or more polypeptide sequences are “substantially identical” if the amino acid residues are about 60% identical, about 65% identical, about 70% identical, about 75% identical, about 80% identical, about 85% identical, about 90% identical, or about 95% identical over a specified region. The identity can exist over a region that is at least about 75-100 amino acids in length, over a region that is about 50 amino acids in length, or, where not specified, across the entire sequence of a polypeptide sequence. In addition, by way of example only, two or more polynucleotide sequences are identical when the nucleic acid residues are the same, while two or more polynucleotide sequences are “substantially identical” if the nucleic acid residues are about 60% identical, about 65% identical, about 70% identical, about 75% identical, about 80% identical, about 85% identical, about 90% identical, or about 95% identical over a specified region. The identity can exist over a region that is at least about 75-100 nucleic acids in length, over a region that is about 50 nucleic acids in length, or, where not specified, across the entire sequence of a polynucleotide sequence.

[00140] The term “isolated,” as used herein, refers to separating and removing a component of interest from components not of interest. Isolated substances can be in either a dry or semi-dry state, or in solution, including but not limited to an aqueous solution. The isolated component can be in a homogeneous state or the isolated component can be a part of a pharmaceutical composition that comprises additional pharmaceutically acceptable carriers and/or excipients. By way of example only, nucleic acids or proteins are “isolated” when such nucleic acids or proteins are free of at least some of the cellular components with which it is associated in the natural state, or that the nucleic acid or protein has been concentrated to a level greater than the concentration of its in vivo or in vitro production. Also, by way of example, a gene is isolated when separated from open reading frames which flank the gene and encode a protein other than the gene of interest.

[00141] A “metabolite” of a compound disclosed herein is a derivative of that compound that is formed when the compound is metabolized. The term “active metabolite” refers to a biologically active derivative of a compound that is formed when the compound is metabolized. The term “metabolized,” as used herein, refers to the sum of the processes (including, but not limited to, hydrolysis reactions and reactions catalyzed by enzymes, such as, oxidation reactions) by which a particular substance is changed by an organism. Thus, enzymes may produce specific structural alterations to a compound. For example, cytochrome P450 catalyzes a variety of oxidative and reductive reactions while uridine diphosphate glucuronyl transferases catalyze the transfer of an activated glucuronic-acid molecule to aromatic alcohols, aliphatic alcohols, carboxylic acids, amines and free sulfhydryl groups. Further information on metabolism may be obtained from The Pharmacological Basis of Therapeutics, 9th Edition, McGraw-Hill (1996). Metabolites of the compounds disclosed herein can be identified either by administration of compounds to a host and analysis of tissue samples from the host, or by incubation of compounds with hepatic cells in vitro and analysis of the resulting compounds. Both methods are well known in the art. In some embodiments, metabolites of a compound are formed by oxidative processes and correspond to the corresponding hydroxy-containing compound. In some embodimets, a compound is metabolized to pharmacologically active metabolites.

[00142] The term “modulate,” as used herein, means to interact with a target either directly or indirectly so as to alter the activity of the target, including, by way of example only, to enhance the activity of the target, to inhibit the activity of the target, to limit the activity of the target, or to extend the activity of the target.

[00143] As used herein, the term “modulator” refers to a compound that alters an activity of a molecule. For example, a modulator can cause an increase or decrease in the magnitude of a certain activity of a molecule compared to the magnitude of the activity in the absence of the modulator. In certain embodiments, a modulator is an inhibitor, which decreases the magnitude of one or more activities of a molecule. In certain embodiments, an inhibitor completely prevents one or more activities of a molecule. In certain embodiments, a modulator is an activator, which increases the magnitude of at least one activity of a molecule. In certain embodiments the presence of a modulator results in an activity that does not occur in the absence of the modulator.

[00144] The term “irreversible inhibitor,” as used herein, refers to a compound that, upon contact with a target protein (e.g., menin) causes the formation of a stable covalent bond with or within the protein. In certain embodiments, one or more of the target protein’s biological activities (e.g., phosphotransferase activity) is diminished or abolished notwithstanding the subsequent presence or absence of the irreversible inhibitor. In certain embodiments, the covalent bond is not reversible under physiological conditions. In certain embodiments, the covalent bond is not reversible in vivo. In contrast, a reversible inhibitor compound upon contact with a target protein does not cause the formation of a new covalent bond with or within the protein and therefore can associate and dissociate from the target potein.

[00145] The term “irreversible inhibitor of menin-MLL protein-proten interaction” as used herein, refers to an inhibitor of menin that can form a covalent bond with an amino acid residue of menin. In one embodiment, the irreversible inhibitor of menin can form a covalent bond with a Cys residue of menin; in particular embodiments, the irreversible inhibitor can form a covalent bond with a Cys 329 residue (or a homolog thereof) of menin.

[00146] The term “prophylactically effective amount,” as used herein, refers that amount of a composition applied to a patient that will relieve to some extent one or more of the symptoms of a disease, condition or disorder being treated. In such prophylactic applications, such amounts may depend on the patient’s state of health, weight, and the like. It is considered well within the skill of the art for one to determine such prophylactically effective amounts by routine experimentation, including, but not limited to, a dose escalation clinical trial.

[00147] As used herein, the term “selective binding compound” refers to a compound that selectively binds to any portion of one or more target proteins.

[00148] As used herein, the term “selectively binds” refers to the ability of a selective binding compound to bind to a target protein, such as, for example, menin, with greater affinity than it binds to a non-target protein. In certain embodiments, specific binding refers to binding to a target with an affinity that is at least 10, 50, 100, 250, 500, 1000 or more times greater than the affinity for a nontarget.

[00149] As used herein, the term “selective modulator” refers to a compound that selectively modulates a target activity relative to a non-target activity. In certain embodiments, specific modulater refers to modulating a target activity at least 10, 50, 100, 250, 500, 1000 times more than a non-target activity.

[00150] The term “substantially purified,” as used herein, refers to a component of interest that may be substantially or essentially free of other components which normally accompany or interact with the component of interest prior to purification. By way of example only, a component of interest may be “substantially purified” when the preparation of the component of interest contains less than about 30%, less than about 25%, less than about 20%, less than about 15%, less than about 10%, less than about 5%, less than about 4%, less than about 3%, less than about 2%, or less than about 1% (by dry weight) of contaminating components. Thus, a “substantially purified” component of interest may have a purity level of about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 96%, about 97%, about 98%, about 99% or greater.

[00151] The term “subject” or “patient” as used herein, refers to an animal which is the object of treatment, observation, or experiment. By way of example only, a subject may be, but is not limited to, a mammal including, but not limited to, a human.

[00152] As used herein, the term “target activity” refers to a biological activity capable of being modulated by a selective modulator. Certain exemplary target activities include, but are not limited to, binding affinity, signal transduction, enzymatic activity, tumor growth, inflammation, or inflammation-related processes, and amelioration of one or more symptoms associated with a disease or condition.

[00153] As used herein, the term “target protein” refers to a molecule or a portion of a protein capable of being bound by a selective binding compound. In certain embodiments, a target protein is menin.

[00154] The terms “treat,” “treating” or “treatment”, as used herein, include alleviating, abating or ameliorating a disease or condition symptoms, preventing additional symptoms, ameliorating or preventing the underlying metabolic causes of symptoms, inhibiting the disease or condition, e.g., arresting the development of the disease or condition, relieving the disease or condition, causing regression of the disease or condition, relieving a condition caused by the disease or condition, or stopping the symptoms of the disease or condition. The terms “treat,” “treating” or “treatment”, include, but are not limited to, prophylactic and/or therapeutic treatments.

[00155] As used herein, the IC50 refers to an amount, concentration, or dosage of a particular test compound that achieves a 50% inhibition of a maximal response, such as inhibition of menin-MLL, in an assay that measures such response. [00156] As used herein, ECso refers to a dosage, concentration, or amount of a particular test compound that elicits a dose-dependent response at 50% of maximal expression of a particular response that is induced, provoked or potentiated by the particular test compound.

[00157] Methods described herein include administering to a subject in need a composition containing a therapeutically effective amount of one or more Menin-MLL inhibitor compounds described herein.

[00158] In some embodiments, methods described herein can be used to treat an autoimmune disease, which includes, but is not limited to, rheumatoid arthritis, psoriatic arthritis, osteoarthritis, Still’s diseasejuvenile arthritis, lupus, diabetes, myasthenia gravis, Hashimoto’s thyroiditis, Ord’s thyroiditis, Graves’ disease Sjogren’s syndrome, multiple sclerosis, Guillain-Barre syndrome, acute disseminated encephalomyelitis, Addison’s disease, opsoclonus-myoclonus syndrome, ankylosing spondylitis, antiphospholipid antibody syndrome, aplastic anemia, autoimmune hepatitis, coeliac disease, Goodpasture’s syndrome, idiopathic thrombocytopenic purpura, optic neuritis, scleroderma, primary biliary cirrhosis, Reiter’s syndrome, Takayasu’s arteritis, temporal arteritis, warm autoimmune hemolytic anemia, Wegener’s granulomatosis, psoriasis, alopecia universalis, Behget’s disease, chronic fatigue, dysautonomia, endometriosis, interstitial cystitis, neuromyotonia, scleroderma, and vulvodynia.

[00159] In some embodiments, methods described herein can be used to treat heteroimmune conditions or diseases, which include, but are not limited to graft versus host disease, transplantation, transfusion, anaphylaxis, allergies (e.g., allergies to plant pollens, latex, drugs, foods, insect poisons, animal hair, animal dander, dust mites, or cockroach calyx), type I hypersensitivity, allergic conjunctivitis, allergic rhinitis, and atopic dermatitis.

[00160] In some embodiments, methods described herein can be used to treat an inflammatory disease, which includes, but is not limited to asthma, inflammatory bowel disease, appendicitis, blepharitis, bronchiolitis, bronchitis, bursitis, cervicitis, cholangitis, cholecystitis, colitis, conjunctivitis, cystitis, dacryoadenitis, dermatitis, dermatomyositis, encephalitis, endocarditis, endometritis, enteritis, enterocolitis, epicondylitis, epididymitis, fasciitis, fibrositis, gastritis, gastroenteritis, hepatitis, hidradenitis suppurativa, laryngitis, mastitis, meningitis, myelitis myocarditis, myositis, nephritis, oophoritis, orchitis, osteitis, otitis, pancreatitis, parotitis, pericarditis, peritonitis, pharyngitis, pleuritis, phlebitis, pneumonitis, pneumonia, proctitis, prostatitis, pyelonephritis, rhinitis, salpingitis, sinusitis, stomatitis, synovitis, tendonitis, tonsillitis, uveitis, vaginitis, vasculitis, and vulvitis. [00161] In some embodiments, methods described herein can be used to treat a cancer, e.g., B-cell proliferative disorders, which include, but are not limited to diffuse large B cell lymphoma, follicular lymphoma, chronic lymphocytic lymphoma, chronic lymphocytic leukemia, B-cell prolymphocytic leukemia, lymphoplasmacytic lymphoma/Waldenstrbm macroglobulinemia, splenic marginal zone lymphoma, plasma cell myeloma, plasmacytoma, extranodal marginal zone B cell lymphoma, nodal marginal zone B cell lymphoma, mantle cell lymphoma, mediastinal (thymic) large B cell lymphoma, intravascular large B cell lymphoma, primary effusion lymphoma, burkitt lymphoma/leukemia, and lymphomatoid granulomatosis.

[00162] In some embodiments, methods described herein can be used to treat thromboembolic disorders, which include, but are not limited to myocardial infarct, angina pectoris (including unstable angina), reocclusions or restenoses after angioplasty or aortocoronary bypass, stroke, transitory ischemia, peripheral arterial occlusive disorders, pulmonary embolisms, and deep venous thromboses.

[00163] Symptoms, diagnostic tests, and prognostic tests for each of the above-mentioned conditions are known in the art. See, e.g., Harrison’s Principles of Internal Medicine®,” 16th ed., 2004, The McGraw-Hill Companies, Inc. Dey et al. (2006), Cytojournal 3(24), and the “Revised European American Lymphoma” (REAL) classification system (see, e.g., the website maintained by the National Cancer Institute).

[00164] A number of animal models of are useful for establishing a range of therapeutically effective doses of Menin-MLL inhibitor compounds for treating any of the foregoing diseases.

[00165] For example, dosing of Menin-MLL inhibitor compounds for treating an autoimmune disease can be assessed in a mouse model of rheumatoid arthitis. In this model, arthritis is induced in Balb/c mice by administering anti-collagen antibodies and lipopolysaccharide. See Nandakumar et al. (2003), Am. J. Pathol 163: 1827-1837.

[00166] In another example, dosing of Menin-MLL irreversible inhibitors for the treatment of B-cell proliferative disorders can be examined in, e.g., a human-to-mouse xenograft model in which human B-cell lymphoma cells (e.g. Ramos cells) are implanted into immunodefficient mice (e.g., “nude” mice) as described in, e.g., Pagel et al. (2005), Clin Cancer Res l l(13):4857-4866.

[00167] Animal models for treatment of thromboembolic disorders are also known.

[00168] The therapeutic efficacy of a provided compound for one of the foregoing diseases can be optimized during a course of treatment. For example, a subject being treated can undergo a diagnostic evaluation to correlate the relief of disease symptoms or pathologies to inhibition of in vivo menin-MLL activity achieved by administering a given dose of an Menin-MLL inhibitor.

Compounds

[00169] In the following description of Menin-MLL inhibitor compounds suitable for use in the methods described herein, definitions of referred-to standard chemistry terms may be found in reference works (if not otherwise defined herein), including Carey and Sundberg “Advanced Organic Chemistry 4th Ed.” Vols. A (2000) and B (2001), Plenum Press, New York. Unless otherwise indicated, conventional methods of mass spectroscopy, NMR, HPLC, protein chemistry, biochemistry, recombinant DNA techniques and pharmacology, within the ordinary skill of the art are employedUnless specific definitions are provided, the nomenclature employed in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those known in the art. Standard techniques can be used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients.

[00170] Menin-MLL inhibitor compounds can be used for the manufacture of a medicament for treating any of the foregoing conditions (e.g., autoimmune diseases, inflammatory diseases, allergy disorders, B-cell proliferative disorders, Myeloid cell proliferative disorder, Lymphoid cell proliferative disorder, or thromboembolic disorders).

[00171] In some embodiments, the Menin-MLL inhibitor compound used for the methods described herein inhibits menin-MLL activity with an in vitro IC50 of less than about 10 pM (e.g., less than about 1 pM, less than about 0.5 pM, less than about 0.4 pM, less than about 0.3 pM, less than about 0.1 pM, less than about 0.08 pM, less than about 0.06 pM, less than about 0.05 pM, less than about 0.04 pM, less than about 0.03 pM, less than about 0.02 pM, less than about 0.01 pM, less than about 0.008 pM, less than about 0.006 pM, less than about 0.005 pM, less than about 0.004 pM, less than about 0.003 pM, less than about 0.002 pM, less than about 0.001 pM, less than about 0.00099 pM, less than about 0.00098 pM, less than about 0.00097 pM, less than about 0.00096 pM, less than about 0.00095 pM, less than about 0.00094 pM, less than about 0.00093 pM, less than about 0.00092 pM, or less than about 0.00090 pM).

[00172] In some embodiments, the Menin-MLL inhibitor compound selectively inhibits an activated form of its target menin.

[00173] Also described herein are methods for synthesizing such irreversible inhibitors, methods for using such irreversible inhibitors in the treatment of diseases (including diseases wherein inhibition of menin-MLL interaction provides therapeutic benefit to a patient having the disease). Further described are pharmaceutical compositions that include an inhibitor of menin-MLL interaction. Specifically, described herein are compounds and methods of use thereof to inhibit interaction of menin with MLL oncoproteins (e.g., MLL1, MLL2, MLL-fusion oncoproteins).

[00174] Specifically described herein are irreversible inhibitors of menin-MLL interaction that form a covalent bond with a cysteine residue on menin. Further described herein are irreversible inhibitors of menin-MLL interaction that form a covalent bond with a Cys329 residue on menin. Also described are pharmaceutical formulations that include a irreversible inhibitor of menin.

[00175] The menin inhibitor compounds described herein are selective for menin having a cysteine residue in an amino acid sequence position of the menin protein that is homologous to the amino acid sequence position of cysteine 329 in menin. Irreversible inhibitor compounds described herein include a Michael acceptor moiety.

[00176] Generally, a reversible or irreversible inhibitor compound of menin used in the methods described herein is identified or characterized in an in vitro assay, e.g., an acellular biochemical assay or a cellular functional assay. Such assays are useful to determine an in vitro IC50 for a reversible or irreversible menin inhibitor compound.

[00177] Further, covalent complex formation between menin and a candidate irreversible menin inhibitor is a useful indicator of irreversible inhibition of menin that can be readily determined by a number of methods known in the art (e.g., mass spectrometry). For example, some irreversible menin-inhibitor compounds can form a covalent bond with Cys 329 of menin (e.g., via a Michael reaction). See S. Xu et al. Angewandte Chemie International Ed. 57(6), 1601-1605 (2017) (incorporated by reference in its entirety).

[00178] Described herein are compounds of any of Formulae (L-I), (L-II), and (I). Also described herein are pharmaceutically acceptable salts, pharmaceutically acceptable solvates, pharmaceutically active metabolites, and pharmaceutically acceptable prodrugs of such compounds. Pharmaceutical compositions that include at least one such compound or a pharmaceutically acceptable salt, pharmaceutically acceptable solvate, pharmaceutically active metabolite or pharmaceutically acceptable prodrug of such compound, are provided. In some embodiments, when compounds disclosed herein contain an oxidizable nitrogen atom, the nitrogen atom can be converted to an N- oxide by methods well known in the art. In certain embodiments, isomers and chemically protected forms of compounds having a structure represented by any of Formula (L-I), (L-II), and (I) are also provided. [00179] In some embodiments, provided herein are menin-MLL irreversible inhibitors according to compounds of formula (L-I).

[00180] In some embodiments, provided herein is a compound according to Formula (L-I) having the structure: or a pharmaceutically acceptable salt thereof, wherein:

Cy ¹ is substituted or unsubstituted

Cy ² is substituted or unsubstituted

Cy ² is substituted or unsubstituted X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(R ^3b ) ₂ -, -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b ) ₂ -, or -O-; or X-W-Y is -N(H)-, or -S(O) ₂ -N(H)-C(R ^3b ) ₂ -;

Cy ³ is substituted or unsubstituted phenylene, pyridylene, pyrimidinylene, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

L is a single bond, substituted or unsubstituted -N(H)-, -C(F) ₂ -O-, or substituted or unsubstituted Ci-4 alkylene;

Cy ⁴ is absent, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

R ¹ is -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-C(O)-C(O)- C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-C(O)-C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -O-C(R ^8a ) ₂ - C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), -N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), - N(R ^3c )-S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), -S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), each R ^8a is independently H or Ci-4 alkyl; each R ^3a , R ^3b , and R ^3c is independently H or substituted or unsubstituted Ci-4 alkyl; each R ^6a and R ^6b is independently H, CN, halo, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond;

R ^6C is H, CN, halo, or Ci-6 alkyl, unsubstituted or substituted with substituted or unsubstituted amino, or substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each R ^6e and R ^6f is independently H, CN, halo, or Ci-6 alkyl;

R ⁷ is H, an optionally substituted group selected from alkyl, a 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, phenyl, an 8-10 membered bicyclic aryl ring, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; and provided that: i. when Cy ¹ is substituted or unsubstituted

-X-W-Y- is -NH-C(O)-; Cy ³ is substituted or unsubstituted phenyl, or substituted or unsubstituted pyrid-2-yl; R ^3c is H; L is -CH2-; Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene; R ¹ is N(H)-C(O)-CR ^6a =CR ^6b R ^6c ; each R ^6a and R ^6b is independently H, or C1-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; and R ^6c is C1-6 alkyl, substituted with substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur; then Cy ² is other than substituted or unsubstituted phenylene; ii. when Cyl is

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b )2-, or -O-;

Cy ³ is substituted or unsubstituted phenylene, or substituted or unsubstituted pyridylene, L is a single bond or -CH2-; and Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene; R ¹ is N(H)-C(O)-CR ^6a =CR ^6b R ^6c ; each R ^6a and R ^6b is independently H, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; and R ^6c is H, or substituted or unsubstituted Ci-6 alkyl; then Cy ² is other than substituted or unsubstituted phenylene, or R ⁷ is other than morpholinyl; or

[00181] In some embodiments, provided herein is a compound according to formula (L-II):

Cy ¹ — Cy ² -X-W-Y-Cy ³ — L — Cy ⁴ — R ¹

(L-ll) or a pharmaceutically acceptable salt thereof, wherein:

Cy ¹ is substituted or unsubstituted

Cy ² is substitute! or unsubstituted

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(R ^3b ) ₂ -, -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b )2-, or -O-; or X-W-Y is -N(H)-, or -S(O) ₂ -N(H)-C(R ^3b ) ₂ -;

Cy ³ is substituted or unsubstituted phenylene, pyridylene, pyrimidinylene, or piperidinylene;

L is a single bond, substituted or unsubstituted -N(H)-, -C(F)2-O-, substituted or unsubstituted Ci-4 alkylene, substituted or unsubstituted C2-4 alkenyl ene, substituted or unsubstituted C2-4 alkynylene, - C(O)-, substituted or unsubstituted cyclopropylene, substituted or unsubstituted cyclobutylene;

Cy ⁴ is absent, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

R ¹ is -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(O)- C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), O-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O) ₂ - C(R ^6a )=C(R ^6b )(R ^6c ), -S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), -S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), each R ^8a is independently H or Ci-4 alkyl; each R ^3a , R ^3b , and R ^3c is independently H or substituted or unsubstituted Ci-4 alkyl; each R ^6a and R ^6b is independently H, CN, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond;

R ^6C is H, CN, or Ci-6 alkyl, unsubstituted or substituted with substituted or unsubstituted amino, or substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each R ^6e and R ^6f is independently H, CN, halo, or Ci-6 alkyl;

R ⁷ is H, an optionally substituted group selected from alkyl, a 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, phenyl, an 8-10 membered bicyclic aryl ring, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; and provided that: i. when Cy ¹ is substituted or unsubstituted

-X-W-Y- is -NH-C(O)-; Cy ³ is substituted or unsubstituted phenyl, or substituted or unsubstituted pyrid-2-yl; R ^3c is H; L is -CH2-; Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene; R ¹ is N(H)-C(O)-CR ^6a =CR ^6b R ^6c ; each R ^6a and R ^6b is independently H, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; and R ^6c is Ci-6 alkyl, substituted with substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur; then Cy ² is other than substituted or unsubstituted phenylene; ii. when Cyl is

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b )2-, or -O-;

Cy ³ is substituted or unsubstituted phenylene, or substituted or unsubstituted pyridylene, L is a single bond or -CH2-; and Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene; R ¹ is N(H)-C(O)-CR ^6a =CR ^6b R ^6c ; each R ^6a and R ^6b is independently H, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; and R ^6c is H, or substituted or unsubstituted Ci-6 alkyl; then Cy ² is other than substituted or unsubstituted phenylene, or R ⁷ is other than morpholinyl; [00182] In some embodiments, provided herein is a compound according to Formula (I) having the structure: or a pharmaceutically acceptable salt thereof, wherein:

Cy ¹ is substituted or unsubstituted

Cy ² is substituted or unsubstituted

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(R ^3b ) ₂ -, -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b )2-, or -O-;

Cy ³ is substituted or unsubstituted phenylene, pyridylene, pyrimidinylene, or piperidinylene;

L is a single bond, substituted or unsubstituted -N(H) -, -C(F)2-O-, or substituted or unsubstituted Ci- 4 alkylene; Cy ⁴ is absent, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

R ¹ is N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-

C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), O-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(R ^8a ) ₂ -

C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), -S(O)-

C(R ^6a )=C(R ^6b )(R ^6c ), -S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), each R ^8a is independently H or Ci-4 alkyl; each R ^3a , R ^3b , and R ^3c is independently H or substituted or unsubstituted Ci-4 alkyl; each R ^6a and R ^6b is independently H, CN, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond;

R ^6C is H, CN, or Ci-6 alkyl, substituted with substituted or unsubstituted amino, or substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ⁷ is an optionally substituted group selected from alkyl, a 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, phenyl, an 8-10 membered bicyclic aryl ring, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; and provided that: i. when Cy ¹ is substituted or unsubstituted

-X-W-Y- is -NH-C(O)-; Cy ³ is substituted or unsubstituted phenyl, or substituted or unsubstituted pyrid-2-yl; R ^3c is H; L is -CH ₂ -; Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene; R1 is N(H)-C(O)-CR ^6a =CR ^6b R ^6c ; each R ^6a and R ^6b is independently H, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; and R ^6c is Ci-6 alkyl, substituted with substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur; then Cy ² is other than substituted or unsubstituted phenylene; ii. when Cy ¹ is

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b )2-, or -O-;

Cy ³ is substituted or unsubstituted phenylene, or substituted or unsubstituted pyridylene, L is a single bond or -CH2-; and Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene; R ¹ is N(H)-C(O)-CR ^6a =CR ^6b R ^6c ; each R ^6a and R ^6b is independently H, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; and R ^6c is H, or substituted or unsubstituted Ci-6 alkyl; then Cy ² is other than substituted or unsubstituted phenylene or R ⁷ is other than morpholinyl; or iii. the compound is:

[00183] In some embodiments, provided herein is a compound according to Formula (I) having the structure: or a pharmaceutically acceptable salt thereof, wherein:

Cy ¹ is substituted or unsubstituted

Cy ² is substituted or unsubstituted

-X-W-Y- is -NH-C(O);

Cy ³ is substituted or unsubstituted phenyl, or substituted or unsubstituted pyridyl;

L is -CH ₂ -;

Cy ⁴ is absent, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene; R ¹ is N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)- C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), O-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(R ^8a ) ₂ - C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), -S(O)-

C(R ^6a )=C(R ^6b )(R ^6c ), -S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), each R ^8a is independently H or C1-4 alkyl; each R ^3a , R ^3b , and R ^3c is independently H or substituted or unsubstituted C1-4 alkyl; each R ^6a and R ^6b is independently H, CN, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond;

R ^6C is H, CN, or Ci-6 alkyl, substituted with substituted or unsubstituted amino, or substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur; and

R ⁷ is an optionally substituted group selected from alkyl, a 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, phenyl, an 8-10 membered bicyclic aryl ring, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

[00184] In some embodiments, provided herein is a compound according to Formula (I) having the structure: or a pharmaceutically acceptable salt thereof, wherein:

Cy ¹ is substituted or unsubstituted

Cy ² is substituted or unsubstituted phenylene;

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(R ^3b ) ₂ -, -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

Cy ³ is substituted or unsubstituted phenylene, or substituted or unsubstituted pyridylene;

L is a single bond or -CH ₂ -;

Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

R ¹ is N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-

C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), O-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(R ^8a ) ₂ -

C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), -S(O)-

C(R ^6a )=C(R ^6b )(R ^6c ), -S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), each R ^8a is independently H or Ci-4 alkyl; each R ^3a , R ^3b , and R ^3c is independently H or substituted or unsubstituted Ci-4 alkyl; each R ^6a and R ^6b is independently H, CN, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond;

R ^6C is Ci-6 alkyl, substituted with substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur;

R ⁷ is an optionally substituted group selected from alkyl, a 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, phenyl, an 8-10 membered bicyclic aryl ring, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

[00185] In some embodiments, i) R ¹ is -C(O)-CH=CH ₂ , -N(H)-C(O)-CH=CH ₂ , -N(Me)-C(O)-CH=CH ₂ , or -N(H)- C(O)-CH=CH-CH ₂ -R ^6C ; or ii) Cy ¹ is v) Cy ⁴ is absent,

vi) L is a single bond, -CH2-, -CH2-CH2-, -C(F2)-O-, or -N(H)-; or vii) -X-W-Y- is -N(H)-C(O)-, -N(H)-C(H)(CF ₃ )-, -N(H)-C(O)-CH ₂ -, -N(H)-C(O)-

N(H)-, -N(H)-C(H)(CF ₃ )-CH ₂ -, or -N(H)-CH ₂ -; or viii) R ⁷ is

A , wherein each A ¹ , A ² , A ³ , A ⁴ , and A ⁵ is independently CR ⁸ R ⁹ , NR ⁸ , CR ⁸ , N, O, or SO ₂ ;

A ⁶ is CR ⁸ , C, or N; each R ⁸ and R ⁹ is independently H, alkyl, or F, and any pair of R ⁸ or R ⁹ may join to form one or more additional rings including fused, bridged, and bicyclic rings; the R ⁷ ring comprises zero, one, or two double bonds, or is aromatic; provided that when A ³ is O and A ⁶ is N, then at least one of A ¹ , A ² , A ⁴ , and A ⁵ is other than -CH2-.

[00186] In some embodiments, Cy ¹ is substituted or unsubstituted [00187] In some embodiments, Cy ¹ is substituted or unsubstituted

[00188] In some embodiments, Cy ¹ is substituted or unsubstituted

[00189] In some embodiments, Cy ¹ is substituted or unsubstituted

[00190] In some embodiments, Cy ¹ is substituted or unsubstituted

[00191] In some embodiments, Cy ² is substituted or unsubstituted

[00192] In some embodiments, Cy ² is substituted or unsubstituted

[00193] In some embodiments, Cy ² is substituted or unsubstituted

[00194] In some embodiments, Cy ² is substituted or unsubstituted

[00195] In some embodiments, Cy ² is substituted or unsubstituted

[00196] In some embodiments, Cy ² is substituted or unsubstituted phenylene, pyridylene, or pyrazinyl.

[00197] In some embodiments, Cy ² is substituted or unsubstituted phenylene.

[00198] In some embodiments, Cy ² is substituted or unsubstituted

[00199] When a bond is depicted between the atoms of one or more rings, it can be bonded to any atom of the ring with a free valence, for instance substituting for a hydrogen atom. When there are two or more rings, fused, spiro, bridging, or otherwise, the bond can be bonded to any atom of any ring with a free valence, for instance substituting for a hydrogen atom, unless specified otherwise. [00200] In some embodiments, Cy ² is substituted or unsubstituted

[00201] In some embodiments, Cy ² is substituted or unsubstituted

[00202] In some embodiments, Cy ² is substituted or unsubstituted

[00203] In some embodiments, Cy ² is substituted or unsubstituted

[00204] In some embodiments, Cy ² is substituted or unsubstituted [00205] In some embodiments, X is N(H) or C(H)2.

[00206] In some embodiments, X is C(H)2.

[00207] In some embodiments, X is N(H).

[00208] In some embodiments, W is C(O), C(H)2, or C(H)(CF3).

[00209] In some embodiments, W is C(O).

[00210] In some embodiments, W is C(H)2

[00211] In some embodiments, W is C(H)(CF3).

[00212] In some embodiments, Y is absent, N(H), or C(H)2.

[00213] In some embodiments, Y is C(H)2.

[00214] In some embodiments, Y is N(H).

[00215] In some embodiments, Y is absent.

[00216] In some embodiments, -X-W-Y- is -N(H)-C(O)-N(H)-, -N(H)-C(O)-CH ₂ -, -CH ₂ -C(O)-

N(H)-, -N(H)-S(O)-N(H)-, -N(H)-S(O)-CH ₂ -, -CH ₂ -S(O)-N(H)-, -N(H)-S(O) ₂ -N(H)-, -N(H)-S(O) ₂ - CH ₂ -, -CH ₂ -S(O) ₂ -N(H)-, -N(H)-C(O)-, -N(H)-C(H) ₂ -, -C(H) ₂ -N(H) ₂ -, or -N(H)-C(H)(CF ₃ )-C(H) ₂ -. [00217] T In some embodiments, -X-W-Y- is -N(H)-C(O)-N(H)-.

[00218] In some embodiments, -X-W-Y- is -N(H)-C(O)-CH2-.

[00219] In some embodiments, -X-W-Y- is -N(H)-C(O)-.

[00220] In some embodiments, -X-W-Y- is -N(H)-C(H)(CF3)-C(H)2-.

[00221] In some embodiments, -X-W-Y- is -N(H)-, or - S(O)2-N(H)-C(R ^3b )2-.

[00222] In some embodiments, -X-W-Y- is -N(H)-.

[00223] In some embodiments, -X-W-Y- is -S(O)2-N(H)-C(H)2-..

[00224] In some embodiments, Cy ³ is substituted or unsubstituted phenyl, pyridyl, or pyrimidinyl.

[00225] In some embodiments, Cy ³ is substituted or unsubstituted piperidinyl.

[00226] In some embodiments, Cy ³ is substituted or unsubstituted pyridyl, or pyrimidinyl, or piperidinyl.

[00227] In some embodiments, Cy ³ is substituted or unsubstituted phenyl, or pyridyl.

[00228] In some embodiments, Cy ³ is substituted or unsubstituted phenyl.

[00229] In some embodiments, Cy ³ is substituted or unsubstituted

[00230] In some embodiments, Cy ³ is substituted or unsubstituted

[00231] In some embodiments, Cy ³ is substituted or unsubstituted

[00232] In some embodiments, Cy ³ is substituted or unsubstituted

[00233] In some embodiments, Cy ³ is substituted or unsubstituted

[00234] In some embodiments, the compound is according to formula (II): or a pharmaceutically acceptable salt thereof; wherein R ¹ , R ⁷ , Cy ² , Cy ³ , Cy ⁴ , X, W, and Y are as described for formula (L-I).

[00235] In some embodiments, the compound is according to formula (II): or a pharmaceutically acceptable salt thereof, wherein:

Cy ² is substituted or unsubstituted

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

W is -C(R ^3b ) ₂ -, -C(O)-, -S(O)-, or -S(O) ₂ -;

Y is absent, -NR ^3a -, -C(R ^3b )2-, or -O-;

Cy ³ is substituted or unsubstituted phenylene, pyridylene, pyrimidinylene, or piperidinylene;

L is a single bond, substituted or unsubstituted -N(H)-, or substituted or unsubstituted Ci-4 alkylene;

Cy ⁴ is absent, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

R ¹ is N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-

C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), O-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(R ^8a ) ₂ -

C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), -S(O)-

C(R ^6a )=C(R ^6b )(R ^6c ), -S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), each R ^8a is independently H or Ci-4 alkyl; each R ^3a , R ^3b , and R ^3c is independently H or substituted or unsubstituted Ci-4 alkyl; each R ^6a and R ^6b is independently H, CN, or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond;

R ^6C is H, CN, or Ci-6 alkyl, substituted with substituted or unsubstituted amino, or substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur; and

R ⁷ is an optionally substituted group selected from alkyl, a 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur, phenyl, an 8-10 membered bicyclic aryl ring, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

[00236] In some embodiments, L is a single bond, or substituted or unsubstituted Ci-4 alkylenyl.

[00237] In some embodiments, L is substituted or unsubstituted -N(H)-.

[00238] In some embodiments, L is a single bond.

[00239] In some embodiments, L is substituted or unsubstituted Ci-4 alkyl enyl.

[00240] In some embodiments, L is -CH2-CH2-.

[00241] In some embodiments, L is -CH2-.

[00242] In some embodiments, L is -C(F2)-O-.

[00243] In some embodiments, L is -N(H)-.

[00244] In some embodiments, Cy ⁴ is substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene.

[00245] In some embodiments, Cy ⁴ is unsubstituted azetidinylene, unsubstituted pyrrolidinylene, unsubstituted piperidinylene, or unsubstituted piperazinylene.

[00246] In some embodiments, Cy ⁴ is azetidinylene, pyrrolidinylene, piperidinylene, or piperazinylene, each substituted with one or more alkylene, halo, or alkoxy. [00247] In some embodiments, Cy ⁴ is azetidinylene, pyrrolidinylene, piperidinylene, or piperazinylene, each substituted with Me, Et, F, (F)i, or Cl.

[00248] In some embodiments, Cy ⁴ is absent.

[00249] In some embodiments, Cy ⁴ is

[00250] In some embodiments, Cy ⁴ is

[00251] In some embodiments, Cy ⁴ is

[00252] In some embodiments, Cy ⁴ is

[00253] In some embodiments, Cy ⁴ is

[00254] In particular embodiments, the compound is according to formula (I): or a pharmaceutically acceptable salt thereof, wherein:

Cy ¹ is substituted or unsubstituted

Cy ² is substituted or unsubstituted phenylene;

X is -NR ^3a -, -C(R ^3b ) ₂ -, or -O-;

Y is a single bond, -NR ^3a -, -C(R ^3b )2-, or -O-;

W is -C(O)-, -S(O)-, or -S(O) ₂ -;

Cy ³ is substituted or unsubstituted phenylene, or pyridylene;

L is a single bond, or -CH ₂ -;

Cy ⁴ is absent, substituted or unsubstituted azetidinylene, substituted or unsubstituted pyrrolidinylene, substituted or unsubstituted piperidinylene, or substituted or unsubstituted piperazinylene;

R ¹ is N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)- C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), O-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ),

N(R ^3c )-S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), or each R ^8a is independently H or Ci-4 alkyl; each R ^6a and R ^6b is independently H or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond;

R ^6C is Ci-6 alkyl, substituted with substituted or unsubstituted amino, or substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur;

R ⁷ is an optionally substituted group selected from alkyl, a 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur, phenyl, an 8-10 membered bicyclic aryl ring, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; provided that -X-W-Y- is other than -NH-C(O)-.

[00255] In some embodiments, i) R ¹ is -C(O)-CH=CH ₂ , -N(H)-C(O)-CH=CH ₂ , -N(Me)-C(O)-CH=CH ₂ , or -N(H)- C(O)-CH=CH-CH ₂ -R ^6C ; or ii) Cy ¹ is iv) Cy ³ is

vi) L is a single bond, -CH2-, -CH2-CH2-, -C(F2)-O-, or -N(H)-; vii) -X-W-Y- is -N(H)-C(O)-, -N(H)-C(H)(CF ₃ )-, -N(H)-C(O)-CH ₂ -, -N(H)-C(O)- N(H)-, -N(H)-C(H)(CF ₃ )-CH ₂ -, or -N(H)-CH ₂ -; or viii) R ⁷ is , wherein each A ¹ , A ² , A ³ , A ⁴ , and A ⁵ is independently CR ⁸ R ⁹ , NR ⁸ , CR ⁸ , N, O, or SO ₂ ;

A ⁶ is CR ⁸ , C, or N; each R ⁸ and R ⁹ is independently H, alkyl, or F, and any pair of R ⁸ or R ⁹ may join to form one or more additional rings including fused, bridged, and bicyclic rings; the R ⁷ ring comprises zero, one, or two double bonds, or is aromatic; provided that when A ³ is O and A ⁶ is N, then at least one of A ¹ , A ² , A ⁴ , and A ⁵ is other than -CH2-.

[00256] In some embodiments, Cy ¹ is substituted or unsubstituted

-X-W-Y- is -NH-C(O); Cy ³ is substituted or unsubstituted phenylene, or pyridylene; and L is -CH2-; and Cy ² is

[00257] In some particular embodiments, R ⁷ -Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00258] In some particular embodiments, R ⁷ -Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00259] In some particular embodiments, R ⁷ -Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00260] In some particular embodiments, R ⁷ -Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00261] In some particular embodiments, R ⁷ -Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00262] In some particular embodiments, R ⁷ -Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00263] In some particular embodiments, R ⁷ -Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00264] In some particular embodiments, R ⁷ -Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00276] In some embodiments, R ⁷ is 4-10 membered heterocycloalkyl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur substituted with Me, Et, or i-Pr. In some embodiments, R ⁷ is substituted with methyl. In some embodiments, R ⁷ is substituted with cyclopropyl. In some embodiments, R ⁷ is fused with cyclopropyl.

[00277] In some embodiments, R ⁷ is a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

[00278] In some embodiments, R ⁷ is , wherein each A ¹ , A ² , A ³ , A ⁴ , and A ⁵ is independently CR ⁸ R ⁹ , NR ⁸ , CR ⁸ , N, O, or SO2; A ⁶ is CR ⁸ , C, or N; each R ⁸ and R ⁹ is independently H or alkyl, and any pair of R ⁸ or R ⁹ may join to form one or more additional rings including fused, bridged, and bicyclic rings; the R ⁷ ring comprises zero, one, or two double bonds, or is aromatic. In some embodiments, when A ³ is O and A ⁶ is N, then at least one of A ¹ , A ² , A ⁴ , and A ⁵ is other than - CH2-. In some embodiments, A ³ is NR ⁸ and A ⁶ is N. In some embodiments, A ³ is O and A ⁶ is CR ⁸ . In some embodiments, A ³ is CR ⁸ and A ⁶ is N. In some embodiments, one or more of A ¹ , A ² , A ³ , A ⁴ , and A ⁵ is CF2. In some embodiments, A ¹ is CF2. In some embodiments, A ³ is CF2. In some embodiments, one or more of A ¹ , A ² , A ³ , A ⁴ , and A ⁵ is CF2. In some embodiments, A ¹ is SO2. In some embodiments, A ³ is SO2.

[00279] In some embodiments, R ⁷ is pyrrolidinyl, piperidinyl, piperazinyl, or morpholinyl.

[00280] In some embodiments, R ⁷ is morpholinyl.

[00281] In some embodiments, R ⁷ is substituted or unsubstituted heteroaryl.

[00282] In some embodiments, R ⁷ is substituted or unsubstituted pyridyl or pyrimidyl.

[00283] In some embodiments, R ⁷ is unsubstituted pyridyl.

[00284] In some embodiments, R ⁷ is pyridyl substituted with halo, hydroxyl, CN, substituted or unsubstituted C1-6 alkyl, substituted or unsubstituted amino, or substituted or unsubstituted alkoxy. [00285] In some embodiments, R ⁷ is pyridyl substituted with Me, Et, i-Pr, OH, Cl, F, CF3, CN, or NH ₂ .

[00286] In some embodiments, R ⁷ is pyridyl substituted with Me, Et, i-Pr, Cl, F, CF3, or CN.

[00287] In some embodiments, R ⁷ is substituted or unsubstituted pyrrolyl, pyrazolyl, imidazolyl, oxazolyl, triazolyl, thiazolyl, oxadiazolyl, or thiadiazolyl.

[00288] In some embodiments, R ⁷ is substituted or unsubstituted imidazolyl.

[00289] In some embodiments, R ⁷ is imidazoyl substituted with Me, Et, i-Pr, Cl, F, CF3, or CN.

[00290] In some embodiments, R ⁷ is imidazoyl substituted with Me.

[00291] In some embodiments, R ⁷ is substituted or unsubstituted alkyl.

[00292] In some embodiments, R ⁷ is Me, Et, n-Pr, i-Pr, or n-Bu.

[00293] In some embodiments, R ⁷ is Me, or Et substituted with halo or hydroxyl.

[00294] In some embodiments, R ⁷ is Me, or Et substituted with Cl or F.

[00295] In some embodiments, R ⁷ is Me, or CF3.

[00296] In some embodiments, R ⁷ is 4-10 membered heterocycloalkyl ring; and the heterocyclic ring is a spiro ring. In some embodiments, the spiro ring is 6:3, 6:4, or 6:5 spiro ring; wherein 6:3 sprio ring is a spiro ring where one ring is 6-membered and other is 3-membered ring; 6:4 sprio ring is a spiro ring where one ring is 6-membered and other is 4-membered ring; and 6:5 sprio ring is a spiro ring where one ring is 6-membered and other is 5-membered ring.

[00297] In some embodiments, R ⁷ is

[00298] In some embodiments, R ⁷ is a spiro ring, and the spiro ring is:

[00299] In some embodiments, R ⁷ is 4-10 membered heterocycloalkyl ring; and the heterocyclic ring is a fused ring. In some embodiments, the fused ring is 6:3, 6:4, or 6:5 ring.

[00300] In some embodiments, R ⁷ is a fused ring, and the fused ring is:

[00301] In some embodiments, R ⁷ is 4-10 membered heterocycloalkyl ring; and the heterocyclic ring is a partially unsaturated ring. In some embodiments, the partially saturated ring has a double bond. In some embodiments, R ⁷ is an partially saturated ring, and the partially saturated ring is:

[00302] In some embodiments, when present the substituted of substituted or unsubstituted group is the group independently substituted with one or more of alkyl, hydroxyl, alkoxy, trifluroalkyl, trifluoroalkoxy, or halo

[00303] In some embodiments, when present substituted Cy ¹ , Cy ² , Cy ³ , or Cy ⁴ is Cy ¹ , Cy ² , Cy ³ , or Cy ⁴ independently substituted with one or more of alkyl, hydroxyl, alkoxy, trifluroalkyl, trifluoroalkoxy, or halo.

[00304] In some embodiments, when present substituted Cy ¹ , Cy ² , Cy ³ , or Cy ⁴ is Cy ¹ , Cy ² , Cy ³ , or Cy ⁴ independently substituted with one or more of Me, Et, i-Pr, CF3, OMe, OEt, OCF3, F, or Cl. [00305] In one embodiment, R ^3c is H. In another embodiment, R ^3c is Me.

[00306] In some particular embodiments, Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00307] In some particular embodiments, Cy ¹ -Cy ² -X-W-Y-Cy ³ is:

[00342] In some embodiments, R ¹ is N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(O)- C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(O)-C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), O-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ), or N(R ^3c )-S(O) ₂ - C(R ^6a )=C(R ^6b )(R ^6c ).

[00343] In some embodiments, R ¹ is N(R ^3c )-S(O) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ).

[00344] In some embodiments, R ¹ is N(R ^3c )-S(O)-C(R ^6a )=C(R ^6b )(R ^6c ).

[00345] In some embodiments, R ¹ is O-C(R ^8a ) ₂ -C(R ^6a )=C(R ^6b )(R ^6c ), or N(R ^3c )-C(R ^8a ) ₂ - C(R ^6a )=C(R ^6b )(R ^6c ); and each R ^8a is H, one of R ^8a is H, and other is Me, or each R ^8a is Me.

[00346] In some embodiments, R ¹ is N(R ^3c )-C(O)-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ), or N(R ^3c )-C(O)- C(R ^8a ) ₂ -C(O)-C(R ^6a )=C(R ^6b )(R ^6c ); and each R ^8a is H, one of R ^8a is H, and other is Me, or each R ^8a is Me.

[00347] In some embodiments, R ¹ is N(R ^3c )-C(O)-C(R ^6a )=C(R ^6b )(R ^6c ).

[00348] In some embodiments, R ^3c is H. [00349] In some embodiments, R ¹ is

[00350] In some embodiments, R ^3c is H, Me, Et, or i-Pr.

[00351] In some embodiments, R ^3c is H.

[00352] In some embodiments, each R ^6a and R ^6b is independently H, CN or Ci-6 alkyl; or R ^6a and R ^6b are joined together to form a bond; R ^6c is H, CN, or Ci-6 alkyl, substituted with substituted or unsubstituted amino, or substituted or unsubstituted heterocycloalkyl, having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

[00353] In some embodiments, each R ^6a and R ^6b is independently H, halo, CN or Ci-6 alkyl.

[00354] In some embodiments, each of R ^6a , R ^6b , and R ^6c is H.

[00355] In some embodiments, R ^6a is F.

[00356] In some embodiments, R ^6a is F, and each of R ^6b , and R ^6c is H

[00357] In some embodiments, R ^6a is F, R ^6b is H; and R ^6c is substituted or unsubstituted alkyl.

[00358] In some embodiments, one of R ^6a , and R ^6b is CN, the other is H; and R ^6c is H, or substituted or unsubstituted alkyl.

[00359] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is unsubstituted alkyl.

[00360] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is substituted or unsubstituted alkyl.

[00361] In some embodiments, one of R ^6a , and R ^6b is CN, the other is H; and R ^6c is H, or substituted or unsubstituted alkyl.

[00362] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is unsubstituted alkyl.

[00363] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is Me, or Et.

[00364] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is alkyl substituted with amino, alkylamino or dialkylamino. [00365] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is alkyl substituted with dimethylamino.

[00366] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is -CH2NMe2.

[00367] In some embodiments, R ^6a , and R ^6b form a bond; and R ^6c is H or substituted or unsubstituted alkyl.

[00368] In some embodiments, R ^6a , and R ^6b form a bond; and R ^6c is Me.

[00369] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is -(CH2) _q -heterocycloalkyl; and q is 1, 2, 3, or 4.

[00370] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is -(CH2) _q -heterocycloalkyl; and q is 1.

[00371] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is -(CH2) _q -heterocycloalkyl; and q is 2.

[00372] In some embodiments, each of R ^6a , and R ^6b is H; and R ^6c is -(CH2) _q -heterocycloalkyl; and q is 3.

[00373] In some embodiments, heterocycloalkyl is substituted or unsubstitued azetidinyl, pyrrolidinyl, piperidinyl, or azepinyl.

[00374] In some embodiments, heterocycloalkyl is azetidin-l-yl, pyrrolidin-l-yl, piperidin-l-yl, or azepin- 1-yl.

[00375] In some embodiments, each of R ^6a , and R ^6b is H or Me; and and R ^6c is -CIL-azetidin-l-yl, - CH2-pyrrolidin-l-yl, or -CIL-piperidin-l-yl.

[00376] In some embodiments, R ¹ is

[00377] In some embodiments, R ¹ is N(R ^3c )-S(O)2-C(R ^6a )=C(R ^6b )(R ^6c ); wherein each of R ^6a and R ^6b is H. In some embodiments, R ¹ is N(R ^3c )-S(O)2-C(H)=C(H)(R ^6c ); and R ^3c is H or Me. In some embodiments, R ¹ is N(H)-S(O)2-C(H)=C(H)(R ^6c ); and R ^6c is H, Me, or alkyl substituted with heterocycloalkyl. In some embodiments, R ¹ is NH-S(O)2-CH=CH2

[00378] In some embodiments, R ¹ is -C(O)-CH=CH ₂ , -N(H)-C(O)-CH=CH ₂ , or -N(Me)-C(O)- CH=CH ₂ . [00379] In some embodiments, R ¹ is -N(H)-C(O)-CH=CH-CH2-R ^6c .

[00380] In some embodiments, each of R ^6a , and R ^6b is H.

[00381] In some embodiments, one of R ^6a , and R ^6b is CN, Me, Et, F, the other is H.

[00382] In some embodiments, R ^6a is H; and R ^6b is alkyl substituted with amino, alkylamino or dialkylamino.

[00383] In some embodiments, R ^6a is H; and R ^6b is -(CH2) _q -heterocycloalkyl; and q is 1, 2, 3, or 4.

[00384] In some embodiments, R ^6a is H; and R ^6b is -(CH2) _q -heterocycloalkyl; and q is 1, 2, 3, or 4; and heterocycloalkyl is azetidin-l-yl, pyrrolidin-l-yl, piperidin-l-yl, or azepin-l-yl.

[00385] In some embodiments, R ^6a is halo.

[00386] In some embodiments, R ^6a is F.

[00387] In some particular embodiments, R ^6a , and R ^6b form a bond; and and R ^6c is Me.

[00388] In some particular embodiments, R ^6c is alkyl, or heterocycloalkylalkyl.

[00389] In some particular embodiments, L-Cy'kR ¹ is -C(O)-CH=CH2, -C(O)-CH=CH-CH2- heterocycloalkyl, -N(H)-C(O)-CH=CH2, or -N(H)-C(O)-CH=CH-CH2-heterocycloalkyl.

[00390] In some particular embodiments, heterocycloalkyl is substituted or unsubstitued azetidine, pyrrolidinyl, or piperidinyl.

[00391] In some particular embodiments, R ^6c is substituted alkyl.

[00392] In some particular embodiments, R ^6c is alkyl substituted with substituted or unsubstituted amino.

[00393] In some particular embodiments, R ^6c is -CH2-NR ¹⁰ R ⁿ , wherein R ¹⁰ is substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heteroalkyl, or substituted or unsubstituted cylcoheteroalkyl, and R ¹¹ is alkyl, substituted alkyl or hydrogen.

[00394] In some particular embodiments, R ^6c is -CH2-NR ¹⁰ R ⁿ , wherein R ¹⁰ is -heteroalkyl-R ¹² , R ¹¹ is alkyl, substituted alkyl or hydrogen, and R ¹² is a label.

[00395] In some particular embodiments, R ^6c is -CH2-NR ¹⁰ R ⁿ , wherein R ¹⁰ is -heteroalkyl-R ¹² , R ¹¹ is alkyl, substituted alkyl or hydrogen, and R ¹² is biotin or BODIPY.

[00396] In some particular embodiments, the compound is selected from any one of compounds listed in Table 1.

[00397] In certain embodiments, the compound is according to formula (I) or any one of the compounds described herein, and wherein at least one of H is replaced with D (deuterium). [00398] In particular embodiments, the compound is

[00399] In particular embodiments, the compound is

[00400] In particular embodiments, the compound is

[00401] Embodiments of the compounds of Formula (I) display improved potency against menin- MLL with IC50 values of as low as less than 1 nM or less than 0.1 nM, and/or high occupancy of active site of menin (e.g., more than 50 %, 70 % or 90% occupancy) at low dosages of below 5 mg/kg (e.g., at or below 3 mg/kg) when administered in vivo (e.g., in rats).

[00402] In some embodiments, provided herein is a pharmaceutical composition comprising a compound according to formula (L-I), (L-II), or (I).

[00403] In some embodiments, provided herein is a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula (L-I), (L-II), or (I), and a pharmaceutically acceptable excipient.

[00404] In some embodiments, the pharmaceutical composition is formulated for a route of administration selected from oral administration, parenteral administration, buccal administration, nasal administration, topical administration, or rectal administration. [00405] In some embodiments, provided herein are methods for treating an autoimmune disease or condition comprising administering to a patient in need the pharmaceutical composition provided herein.

[00406] In some embodiments, the autoimmune disease is selected from rheumatoid arthritis or lupus.

[00407] In some embodiments, provided herein are methods for treating a heteroimmune disease or condition comprising administering to a patient in need the pharmaceutical composition provided herein.

[00408] In some embodiments, provided herein are methods for treating a cancer comprising administering to a patient in need the pharmaceutical composition provided herein.

[00409] In some embodiments, the cancer is a B-cell proliferative disorder.

[00410] In some embodiments, the B-cell proliferative disorder is diffuse large B cell lymphoma, follicular lymphoma, or chronic lymphocytic leukemia. In some embodiments, the disorder is myeloid leukemia. In some embodiments, the disorder is acute myeloid leukemia (AML). In some embodiments, the B-cell proliferative disorder is lymphoid leukemia. In some embodiments, the disorder is acute lymphocytic leukemia (ALL). In some embodiments, the disorder is soft tissue tumors. In some embodiments, the tumor is glioblastoma. In some embodiments, the tumor is pancreatic tumor. In some embodiments, the disorder is renal cell cancer.

[00411] In some embodiments, the disorder is KRas mutated solid tumors.

[00412] In some embodiments, the disorder is multiple myeloma.

[00413] In some embodiments, the disorder is Triple-Hit Lymphoma (THL).

[00414] In some embodiments, the disorder is Double Expresser Lymphoma (DEL). In some embodiments, the disorder is DLBCL.

[00415] In some embodiments, provided herein are methods for treating mastocytosis comprising administering to a patient in need the pharmaceutical composition provided herein.

[00416] In some embodiments, provided herein are methods for treating osteoporosis or bone resorption disorders comprising administering to a patient in need the pharmaceutical composition provided herein.

[00417] In some embodiments, provided herein are methods for treating an inflammatory disease or condition comprising administering to a patient in need the pharmaceutical composition provided herein. [00418] In some embodiments, provided herein are methods for treating lupus comprising administering to a subject in need thereof a composition containing a therapeutically effective amount of a compound of formula (L-I), (L-II), or (I)that is inhibitor of menin-MLL interaction. [00419] In some embodiments, provided herein are methods for treating a heteroimmune disease or condition comprising administering to a subject in need thereof a composition containing a therapeutically effective amount of a compound of formula (L-I), (L-II), or (I) that is inhibitor of menin-MLL interaction.

[00420] In some embodiments provided herein are methods for treating diffuse large B cell lymphoma, follicular lymphoma or chronic lymphocytic leukemia comprising administering to a subject in need thereof a composition containing a therapeutically effective amount of a compound of formula (L-I), (L-II), or (I) that is inhibitor of the menin-MLL interaction.

[00421] In some embodiments, provided herein are methods for treating mastocytosis, comprising administering to a subject in need thereof a composition containing a therapeutically effective amount of a compound of formula (L-I), (L-II), or (I) that is inhibitor of menin-MLL interaction. [00422] In some embodiments, provided herein are methods for treating osteoporosis or bone resorption disorders comprising administering to a subject in need thereof a composition containing a therapeutically effective amount of a compound of formula (L-I), (L-II), or (I) that is inhibitor of menin-MLL interaction.

[00423] In some embodiments, provided herein are methods for treating an inflammatory disease or condition comprising administering to a subject in need thereof a composition containing a therapeutically effective amount of a compound of formula (L-I), (L-II), or (I) that is inhibitor of menin-MLL interaction.

[00424] In some embodiments, provided herein is a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a pharmaceutically effective amount of a compound according to any one of the formulas described herein. In some embodiments, the compound is according to any one of Formula (L-I), (L-II), or (I).

[00425] In some embodiments, the pharmaceutical composition is formulated for a route of administration selected from oral administration, parenteral administration, buccal administration, nasal administration, topical administration, or rectal administration.

[00426] In some embodiments, the carrier is a parenteral carrier.

[00427] In some embodiments, the carrier is an oral carrier. [00428] In some embodiments, the carrier is a topical carrier. [00429] Any combination of the groups described above for the various variables is contemplated herein. It is understood that substituents and substitution patterns on the compounds provided herein can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be synthesized by techniques known in the art, as well as those set forth herein.

[00430] Further representative embodiments of compounds of Formula (I), include compounds listed in Table 1, or a pharmaceutically acceptable salt, solvate, hydrate, or stereoisomer thereof.

[00431] Throughout the specification, groups and substituents thereof can be chosen by one skilled in the field to provide stable moieties and compounds.

[00432] In some embodiments, the compounds of Formula (L-I), (L-II), and (I) inhibit menin-MLL. In some embodiments, the compounds of Formula (L-I), (L-II), and (I) are used to treat patients suffering from menin-MLL-dependent or menin-MLL interaction mediated conditions or diseases, including, but not limited to, cancer, autoimmune and other inflammatory diseases.

[00433] In some embodiments, the compounds of Formula (L-I), (L-II), and (I) inhibit menin-MLL interaction. In some embodiments, the compounds of Formula (L-I), (L-II), and (I) are used to treat patients suffering from menin-MLL interaction-dependent or menin-MLL interaction mediated conditions or diseases, including, but not limited to, cancer, autoimmune and other inflammatory diseases.

Preparation of Compounds

[00434] Compounds of any of Formula (L-I), (L-II), and (I) may be synthesized using standard synthetic reactions known to those of skill in the art or using methods known in the art. The reactions can be employed in a linear sequence to provide the compounds or they may be used to synthesize fragments which are subsequently joined by the methods known in the art. Exemplary methods are provided in the Examples herein.

[00435] Described herein are compounds that inhibit the activity of menin-MLL, and processes for their preparation. Also described herein are pharmaceutically acceptable salts, pharmaceutically acceptable solvates, pharmaceutically active metabolites, and pharmaceutically acceptable prodrugs of such compounds. Pharmaceutical compositions that include at least one such compound or a pharmaceutically acceptable salt, pharmaceutically acceptable solvate, pharmaceutically active metabolite or pharmaceutically acceptable prodrug of such compound, are provided.

[00436] The starting material used for the synthesis of the compounds described herein may be synthesized or can be obtained from commercial sources, such as, but not limited to, Aldrich Chemical Co. (Milwaukee, Wisconsin), Bachem (Torrance, California), or Sigma Chemical Co. (St. Louis, Mo.). The compounds described herein, and other related compounds having different substituents can be synthesized using techniques and materials known to those of skill in the art, such as described, for example, in March, ADVANCED ORGANIC CHEMISTRY 4 ^th Ed., (Wiley 1992); Carey and Sundberg, ADVANCED ORGANIC CHEMISTRY 4 ^th Ed., Vols. A and B (Plenum 2000, 2001); Green and Wuts, PROTECTIVE GROUPS IN ORGANIC SYNTHESIS 3 ^rd Ed., (Wiley 1999); Fieser and Fieser’s Reagents for Organic Synthesis, Volumes 1-17 (John Wiley and Sons, 1991); Rodd’s Chemistry of Carbon Compounds, Volumes 1-5 and Suppiementals (Elsevier Science Publishers, 1989); Organic Reactions, Volumes 1-40 (John Wiley and Sons, 1991); and Larock’s Comprehensive Organic Transformations (VCH Publishers Inc., 1989). (all of which are incorporated by reference in their entirety). Additional methods for the synthesis of compounds described herein may be found in International Patent Publication No. WO 01/01982901, Arnold et al. Bioorganic & Medicinal Chemistry Letters 10 (2000) 2167-2170; Burchat et al. Bioorganic & Medicinal Chemistry Letters 12 (2002) 1687-1690. General methods for the preparation of compound as disclosed herein may be derived from known reactions in the field, and the reactions may be modified by the use of appropriate reagents and conditions, as would be recognized by the skilled person, for the introduction of the various moieties found in the formulae as provided herein. [00437] The products of the reactions may be isolated and purified, if desired, using conventional techniques, including, but not limited to, filtration, distillation, crystallization, chromatography, and the like. Such materials may be characterized using conventional means, including physical constants and spectral data.

[00438] Compounds described herein may be prepared as a single isomer or a mixture of isomers. [00439] In some embodiments, representative compounds of Formula (I) are prepared according to synthetic schemes depicted herein.

Further Forms of Compounds

[00440] Compounds disclosed herein have a structure of Formula (L-I), (L-II), and (I). It is understood that when reference is made to compounds described herein, it is meant to include compounds of any of Formula (L-I), (L-II), and (I) as well as to all of the specific compounds that fall within the scope of these generic formulae, unless otherwise indicated.

[00441] Compounds described herein may possess one or more stereocenters and each center may exist in the R or S configuration. Compounds presented herein include all diastereomeric, enantiomeric, and epimeric forms as well as the appropriate mixtures thereof. Stereoisomers may be obtained, if desired, by methods known in the art as, for example, the separation of stereoisomers by chiral chromatographic columns.

[00442 J Diastereomeric mixtures can be separated into their individual diastereomers on the basis of their physical chemical differences by methods known, for example, by chromatography and/or fractional crystallization. In some embodiments, enantiomers can be separated by chiral chromatographic columns. In some embodiments, enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g., alcohol), separating the diastereomers and converting (e.g., hydrolyzing) the individual diastereomers to the corresponding pure enantiomers. All such isomers, including diastereomers, enantiomers, and mixtures thereof are considered as part of the compositions described herein.

[00443] Methods and formulations described herein include the use of N-oxides, crystalline forms (also known as polymorphs), or pharmaceutically acceptable salts of compounds described herein, as well as active metabolites of these compounds having the same type of activity. In some situations, compounds may exist as tautomers. All tautomers are included within the scope of the compounds presented herein. In addition, compounds described herein can exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like. Solvated forms of compounds presented herein are also considered to be disclosed herein.

[00444] Compounds of any of Formula (L-I), (L-II), and (I) in unoxidized form can be prepared from N-oxides of compounds of any of Formula (L-I), (L-II), and (I) by treating with a reducing agent, such as, but not limited to, sulfur, sulfur dioxide, triphenyl phosphine, lithium borohydride, sodium borohydride, phosphorus trichloride, tribromide, or the like in a suitable inert organic solvent, such as, but not limited to, acetonitrile, ethanol, aqueous dioxane, or the like at 0 to 80°C. [00445] In some embodiments, compounds described herein are prepared as prodrugs. A “prodrug” refers to an agent that is converted into the parent drug in vivo. Prodrugs are often useful because, in some situations, they may be easier to administer than the parent drug. They may, for instance, be bioavailable by oral administration whereas the parent is not. The prodrug may also have improved solubility in pharmaceutical compositions over the parent drug. An example, without limitation, of a prodrug would be a compound described herein, which is administered as an ester (the “prodrug”) to facilitate transmittal across a cell membrane where water solubility is detrimental to mobility but which then is metabolically hydrolyzed to the carboxylic acid, the active entity, once inside the cell where water-solubility is beneficial. A further example of a prodrug might be a short peptide (polyaminoacid) bonded to an acid group where the peptide is metabolized to reveal the active moiety. In certain embodiments, upon in vivo administration, a prodrug is chemically converted to the biologically, pharmaceutically, or therapeutically active form of the compound. In certain embodiments, a prodrug is enzymatically metabolized by one or more steps or processes to the biologically, pharmaceutically, or therapeutically active form of the compound. To produce a prodrug, a pharmaceutically active compound is modified such that the active compound will be regenerated upon in vivo administration. The prodrug can be designed to alter the metabolic stability or the transport characteristics of a drug, to mask side effects or toxicity, to improve the flavor of a drug or to alter other characteristics or properties of a drug. By virtue of knowledge of pharmacodynamic processes and drug metabolism in vivo, those of skill in this art, once a pharmaceutically active compound is known, can design prodrugs of the compound, (see, for example, Nogrady (1985) Medicinal Chemistry A Biochemical Approach, Oxford University Press, New York, pages 388-392; Silverman (1992), The Organic Chemistry of Drug Design and Drug Action, Academic Press, Inc., San Diego, pages 352-401, Saulnier et al., (1994), Bioorganic and Medicinal Chemistry Letters, Vol. 4, p. 1985).

[00446] Prodrug forms of the herein described compounds, wherein the prodrug is metabolized in vivo to produce a derivative as set forth herein are included within the scope of the claims. In some cases, some of the herein-described compounds may be a prodrug for another derivative or active compound.

[00447] Prodrugs are often useful because, in some situations, they may be easier to administer than the parent drug. They may, for instance, be bioavailable by oral administration whereas the parent is not. The prodrug may also have improved solubility in pharmaceutical compositions over the parent drug. Prodrugs may be designed as reversible drug derivatives, for use as modifiers to enhance drug transport to site-specific tissues. In some embodiments, the design of a prodrug increases the effective water solubility. See, e.g., Fedorak et al., Am. J. Physiol., 269:G210-218 (1995); McLoed et al., Gastroenterol, 106:405-413 (1994); Hochhaus et al., Biomed. Chrom., 6:283-286 (1992); J. Larsen and H. Bundgaard, Int. J. Pharmaceutics, 37, 87 (1987); J. Larsen et al., Int. J. Pharmaceutics, 47, 103 (1988); Sinkula et al., J. Pharm. Sci., 64: 181-210 (1975); T. Higuchi and V. Stella, Pro-drugs as Novel Delivery Systems, Vol. 14 of the A.C.S. Symposium Series; and Edward B. Roche, Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987, all incorporated herein in their entirety. [00448] Sites on the aromatic ring portion of compounds of any of Formula (L-I), (L-II), and (I) can be susceptible to various metabolic reactions, therefore incorporation of appropriate substituents on the aromatic ring structures, such as, by way of example only, halogens can reduce, minimize or eliminate this metabolic pathway.

[00449] Compounds described herein include isotopically-labeled compounds, which are identical to those recited in the various formulas and structures presented herein, but for the fact that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into the present compounds include isotopes of hydrogen, carbon, nitrogen, oxygen, fluorine and chlorine, such as ² H, ³ H, ¹³ C, ¹⁴ C, ¹⁵ N, ¹⁸ O, ¹⁷ 0, ³⁵ S, ¹⁸ F, ³⁶ C1, respectively. Certain isotopically-labeled compounds described herein, for example those into which radioactive isotopes such as ³ H and ¹⁴ C are incorporated, are useful in drug and/or substrate tissue distribution assays. Further, substitution with isotopes such as deuterium, i.e., ² H, can afford certain therapeutic advantages resulting from greater metabolic stability, for example increased in vivo half-life or reduced dosage requirements. [00450] In additional or some embodiments, the compounds described herein are metabolized upon administration to an organism in need to produce a metabolite that is then used to produce a desired effect, including a desired therapeutic effect.

[00451] Compounds described herein may be formed as, and/or used as, pharmaceutically acceptable salts. The type of pharmaceutical acceptable salts, include, but are not limited to: (1) acid addition salts, formed by reacting the free base form of the compound with a pharmaceutically acceptable: inorganic acid such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, metaphosphoric acid, and the like; or with an organic acid such as acetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, trifluoroacetic acid, tartaric acid, citric acid, benzoic acid, 3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid, toluenesulfonic acid, 2-naphthalenesulfonic acid, 4-methylbicyclo-[2.2.2]oct-2-ene-l- carboxylic acid, glucoheptonic acid, 4,4’-methylenebis-(3-hydroxy-2-ene-l -carboxylic acid), 3- phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid, lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylic acid, stearic acid, muconic acid, and the like;

(2) salts formed when an acidic proton present in the parent compound either is replaced by a metal ion, e.g., an alkali metal ion (e.g. lithium, sodium, potassium), an alkaline earth ion (e.g. magnesium, or calcium), or an aluminum ion; or coordinates with an organic base. Acceptable organic bases include ethanolamine, diethanolamine, triethanolamine, tromethamine, N-methylglucamine, and the like. Acceptable inorganic bases include aluminum hydroxide, calcium hydroxide, potassium hydroxide, sodium carbonate, sodium hydroxide, and the like.

[00452] The corresponding counterions of the pharmaceutically acceptable salts may be analyzed and identified using various methods including, but not limited to, ion exchange chromatography, ion chromatography, capillary electrophoresis, inductively coupled plasma, atomic absorption spectroscopy, mass spectrometry, or any combination thereof.

[00453] The salts are recovered by using at least one of the following techniques: filtration, precipitation with a non-solvent followed by filtration, evaporation of the solvent, or, in the case of aqueous solutions, lyophilization.

[00454] It should be understood that a reference to a pharmaceutically acceptable salt includes the solvent addition forms or crystal forms thereof, particularly solvates or polymorphs. Solvates contain either stoichiometric or non-stoichiometric amounts of a solvent, and may be formed during the process of crystallization with pharmaceutically acceptable solvents such as water, ethanol, and the like. Hydrates are formed when the solvent is water, or alcoholates are formed when the solvent is alcohol. Solvates of compounds described herein can be conveniently prepared or formed during the processes described herein. In addition, the compounds provided herein can exist in unsolvated as well as solvated forms. In general, the solvated forms are considered equivalent to the unsolvated forms for the purposes of the compounds and methods provided herein.

[00455] It should be understood that a reference to a salt includes the solvent addition forms or crystal forms thereof, particularly solvates or polymorphs. Solvates contain either stoichiometric or non-stoichiometric amounts of a solvent, and are often formed during the process of crystallization with pharmaceutically acceptable solvents such as water, ethanol, and the like. Hydrates are formed when the solvent is water, or alcoholates are formed when the solvent is alcohol. Polymorphs include the different crystal packing arrangements of the same elemental composition of a compound. Polymorphs usually have different X-ray diffraction patterns, infrared spectra, melting points, density, hardness, crystal shape, optical and electrical properties, stability, and solubility. Various factors such as the recrystallization solvent, rate of crystallization, and storage temperature may cause a single crystal form to dominate.

[00456] Compounds described herein may be in various forms, including but not limited to, amorphous forms, milled forms and nano-particulate forms. In addition, compounds described herein include crystalline forms, also known as polymorphs. Polymorphs include the different crystal packing arrangements of the same elemental composition of a compound. Polymorphs usually have different X-ray diffraction patterns, infrared spectra, melting points, density, hardness, crystal shape, optical and electrical properties, stability, and solubility. Various factors such as the recrystallization solvent, rate of crystallization, and storage temperature may cause a single crystal form to dominate. [00457] The screening and characterization of the pharmaceutically acceptable salts, polymorphs, and/or solvates may be accomplished using a variety of techniques including, but not limited to, thermal analysis, x-ray diffraction, spectroscopy, vapor sorption, and microscopy. Thermal analysis methods address thermo chemical degradation or thermo physical processes including, but not limited to, polymorphic transitions, and such methods are used to analyze the relationships between polymorphic forms, determine weight loss, to find the glass transition temperature, or for excipient compatibility studies. Such methods include, but are not limited to, Differential scanning calorimetry (DSC), Modulated Differential Scanning Calorimetry (MDCS), Thermogravimetric analysis (TGA), and Thermogravi-metric and Infrared analysis (TG/IR). X-ray diffraction methods include, but are not limited to, single crystal and powder diffractometers and synchrotron sources. The various spectroscopic techniques used include, but are not limited to, Raman, FTIR, UVIS, and NMR (liquid and solid state). The various microscopy techniques include, but are not limited to, polarized light microscopy, Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDX), Environmental Scanning Electron Microscopy with EDX (in gas or water vapor atmosphere), IR microscopy, and Raman microscopy.

[00458] Throughout the specification, groups and substituents thereof can be chosen by one skilled in the field to provide stable moieties and compounds.

Pharmaceutical Composition/Formulation

[00459] Pharmaceutical compositions may be formulated in a conventional manner using one or more physiologically acceptable carriers including excipients and auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutically. Proper formulation is dependent upon the route of administration chosen. Any of the well-known techniques, carriers, and excipients may be used as suitable and as understood in the art. A summary of pharmaceutical compositions described herein may be found, for example, in Remington: The Science and Practice of Pharmacy, Nineteenth Ed (Easton, Pa.: Mack Publishing Company, 1995); Hoover, John E., Remington’s Pharmaceutical Sciences, Mack Publishing Co., Easton, Pennsylvania 1975; Liberman, H.A. and Lachman, L., Eds., Pharmaceutical Dosage Forms, Marcel Decker, New York, N.Y., 1980; and Pharmaceutical Dosage Forms and Drug Delivery Systems, Seventh Ed. (Lippincott Williams & Wilkins 1999), herein incorporated by reference in their entirety.

[00460] A pharmaceutical composition, as used herein, refers to a mixture of a compound described herein, such as, for example, compounds of any of Formula (L-I), (L-II), and (I) with other chemical components, such as carriers, stabilizers, diluents, dispersing agents, suspending agents, thickening agents, and/or excipients. The pharmaceutical composition facilitates administration of the compound to an organism. In practicing the methods of treatment or use provided herein, therapeutically effective amounts of compounds described herein are administered in a pharmaceutical composition to a mammal having a disease, disorder, or condition to be treated. Preferably, the mammal is a human. A therapeutically effective amount can vary widely depending on the severity of the disease, the age and relative health of the subject, the potency of the compound used and other factors. The compounds can be used singly or in combination with one or more therapeutic agents as components of mixtures.

[00461] In certain embodiments, compositions may also include one or more pH adjusting agents or buffering agents, including acids such as acetic, boric, citric, lactic, phosphoric and hydrochloric acids; bases such as sodium hydroxide, sodium phosphate, sodium borate, sodium citrate, sodium acetate, sodium lactate and tris-hydroxymethylaminomethane; and buffers such as citrate/dextrose, sodium bicarbonate and ammonium chloride. Such acids, bases and buffers are included in an amount required to maintain pH of the composition in an acceptable range.

[00462] In some embodiments, compositions may also include one or more salts in an amount required to bring osmolality of the composition into an acceptable range. Such salts include those having sodium, potassium or ammonium cations, and chloride, citrate, ascorbate, borate, phosphate, bicarbonate, sulfate, thiosulfate or bisulfite anions; suitable salts include sodium chloride, potassium chloride, sodium thiosulfate, sodium bisulfite and ammonium sulfate.

[00463] The term “pharmaceutical combination” as used herein, means a product that results from the mixing or combining of more than one active ingredient and includes both fixed and non-fixed combinations of the active ingredients. The term “fixed combination” means that the active ingredients, e.g. a compound described herein and a co-agent, are both administered to a patient simultaneously in the form of a single entity or dosage. The term “non-fixed combination” means that the active ingredients, e.g. a compound described herein and a co-agent, are administered to a patient as separate entities either simultaneously, concurrently or sequentially with no specific intervening time limits, wherein such administration provides effective levels of the two compounds in the body of the patient. The latter also applies to cocktail therapy, e.g. the administration of three or more active ingredients.

[00464] The pharmaceutical compositions described herein can be administered to a subject by multiple administration routes, including but not limited to, oral, parenteral (e.g., intravenous, subcutaneous, intramuscular), intranasal, buccal, topical, rectal, or transdermal administration routes. The pharmaceutical compositions described herein include, but are not limited to, aqueous liquid dispersions, self-emulsifying dispersions, solid solutions, liposomal dispersions, aerosols, solid dosage forms, powders, immediate release formulations, controlled release formulations, fast melt formulations, tablets, capsules, pills, delayed release formulations, extended release formulations, pulsatile release formulations, multiparticulate formulations, and mixed immediate and controlled release formulations.

[00465] Pharmaceutical compositions including a compound described herein may be manufactured in a conventional manner, such as, by way of example only, by means of conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping or compression processes.

[00466] The pharmaceutical compositions will include at least one compound described herein, such as, for example, a compound of any of Formula (L-I), (L-II), and (I) as an active ingredient in free- acid or free-base form, or in a pharmaceutically acceptable salt form. In addition, the methods and pharmaceutical compositions described herein include the use of N-oxides, crystalline forms (also known as polymorphs), as well as active metabolites of these compounds having the same type of activity. In some situations, compounds may exist as tautomers. All tautomers are included within the scope of the compounds presented herein. Additionally, the compounds described herein can exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like. The solvated forms of the compounds presented herein are also considered to be disclosed herein.

[00467] “Antifoaming agents” reduce foaming during processing which can result in coagulation of aqueous dispersions, bubbles in the finished film, or generally impair processing. Exemplary antifoaming agents include silicon emulsions or sorbitan sesquoleate.

[00468] “Antioxidants” include, for example, butylated hydroxytoluene (BHT), sodium ascorbate, ascorbic acid, sodium metabisulfite, and tocopherol. In certain embodiments, antioxidants enhance chemical stability where required. [00469] In certain embodiments, compositions provided herein may also include one or more preservatives to inhibit microbial activity. Suitable preservatives include mercury-containing substances such as merfen and thiomersal; stabilized chlorine dioxide; and quaternary ammonium compounds such as benzalkonium chloride, cetyltrimethylammonium bromide and cetylpyridinium chloride.

[00470] Formulations described herein may benefit from antioxidants, metal chelating agents, thiol containing compounds and other general stabilizing agents. Examples of such stabilizing agents, include, but are not limited to: (a) about 0.5% to about 2% w/v glycerol, (b) about 0.1% to about 1% w/v methionine, (c) about 0.1% to about 2% w/v monothioglycerol, (d) about 1 mM to about 10 mM EDTA, (e) about 0.01% to about 2% w/v ascorbic acid, (f) 0.003% to about 0.02% w/v polysorbate 80, (g) 0.001% to about 0.05% w/v. polysorbate 20, (h) arginine, (i) heparin, (j) dextran sulfate, (k) cyclodextrins, (1) pentosan polysulfate and other heparinoids, (m) divalent cations such as magnesium and zinc; or (n) combinations thereof.

[00471] “Binders” impart cohesive qualities and include, e.g., alginic acid and salts thereof; cellulose derivatives such as carboxymethylcellulose, methylcellulose (e.g., Methocel®), hydroxypropylmethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose (e.g., Klucel®), ethylcellulose (e.g., Ethocel®), and microcrystalline cellulose (e.g., Avicel®); microcrystalline dextrose; amylose; magnesium aluminum silicate; polysaccharide acids; bentonites; gelatin; polyvinylpyrrolidone/vinyl acetate copolymer; crosspovidone; povidone; starch; pregelatinized starch; tragacanth, dextrin, a sugar, such as sucrose (e.g., Dipac®), glucose, dextrose, molasses, mannitol, sorbitol, xylitol (e.g., Xylitab®), and lactose; a natural or synthetic gum such as acacia, tragacanth, ghatti gum, mucilage of isapol husks, polyvinylpyrrolidone (e.g., Polyvidone® CL, Kollidon® CL, Polyplasdone® XL- 10), larch arabogalactan, Veegum®, polyethylene glycol, waxes, sodium alginate, and the like.

[00472] A “carrier” or “carrier materials” include any commonly used excipients in pharmaceutics and should be selected on the basis of compatibility with compounds disclosed herein, such as, compounds of any of Formula (L-I), (L-II), and (I) and the release profile properties of the desired dosage form. Exemplary carrier materials include, e.g., binders, suspending agents, disintegration agents, filling agents, surfactants, solubilizers, stabilizers, lubricants, wetting agents, diluents, and the like. “Pharmaceutically compatible carrier materials” may include, but are not limited to, acacia, gelatin, colloidal silicon dioxide, calcium glycerophosphate, calcium lactate, maltodextrin, glycerine, magnesium silicate, polyvinylpyrrollidone (PVP), cholesterol, cholesterol esters, sodium caseinate, soy lecithin, taurocholic acid, phosphotidylcholine, sodium chloride, tricalcium phosphate, dipotassium phosphate, cellulose and cellulose conjugates, sugars sodium stearoyl lactylate, carrageenan, monoglyceride, diglyceride, pregelatinized starch, and the like. See, e.g., Remington: The Science and Practice of Pharmacy, Nineteenth Ed (Easton, Pa.: Mack Publishing Company, 1995); Hoover, John E., Remington’s Pharmaceutical Sciences, Mack Publishing Co., Easton, Pennsylvania 1975; Liberman, H.A. and Lachman, L., Eds., Pharmaceutical Dosage Forms, Marcel Decker, New York, N.Y., 1980; and Pharmaceutical Dosage Forms and Drug Delivery Systems, Seventh Ed. (Lippincott Williams & Wilkinsl999).

[00473] “Dispersing agents,” and/or “viscosity modulating agents” include materials that control the diffusion and homogeneity of a drug through liquid media or a granulation method or blend method. In some embodiments, these agents also facilitate the effectiveness of a coating or eroding matrix. Exemplary diffusion facilitators/dispersing agents include, e.g., hydrophilic polymers, electrolytes, Tween ® 60 or 80, PEG, polyvinylpyrrolidone (PVP; commercially known as Plasdone®), and the carbohydrate-based dispersing agents such as, for example, hydroxypropyl celluloses (e.g., HPC, HPC-SL, and HPC-L), hydroxypropyl methylcelluloses (e.g., HPMC K100, HPMC K4M, HPMC K15M, and HPMC K100M), carboxymethylcellulose sodium, methylcellulose, hydroxyethylcellulose, hydroxypropyl cellulose, hydroxypropylmethylcellulose phthalate, hydroxypropylmethylcellulose acetate stearate (HPMCAS), noncrystalline cellulose, magnesium aluminum silicate, triethanolamine, polyvinyl alcohol (PVA), vinyl pyrrolidone/vinyl acetate copolymer (S630), 4-(l,l,3,3-tetramethylbutyl)-phenol polymer with ethylene oxide and formaldehyde (also known as tyloxapol), poloxamers (e.g., Pluronics F68®, F88®, and F108®, which are block copolymers of ethylene oxide and propylene oxide); and poloxamines (e.g., Tetronic 908®, also known as Poloxamine 908®, which is a tetrafunctional block copolymer derived from sequential addition of propylene oxide and ethylene oxide to ethylenediamine (BASF Corporation, Parsippany, N.J.)), polyvinylpyrrolidone K12, polyvinylpyrrolidone K17, polyvinylpyrrolidone K25, or polyvinylpyrrolidone K30, polyvinylpyrrolidone/vinyl acetate copolymer (S-630), polyethylene glycol, e.g., the polyethylene glycol can have a molecular weight of about 300 to about 6000, or about 3350 to about 4000, or about 7000 to about 5400, sodium carboxymethylcellulose, methylcellulose, polysorbate-80, sodium alginate, gums, such as, e.g., gum tragacanth and gum acacia, guar gum, xanthans, including xanthan gum, sugars, cellulosics, such as, e.g., sodium carboxymethylcellulose, methylcellulose, sodium carboxymethylcellulose, polysorbate-80, sodium alginate, polyethoxylated sorbitan monolaurate, polyethoxylated sorbitan monolaurate, povidone, carbomers, polyvinyl alcohol (PVA), alginates, chitosans and combinations thereof. Plasticizcers such as cellulose or triethyl cellulose can also be used as dispersing agents. Dispersing agents particularly useful in liposomal dispersions and self-emulsifying dispersions are dimyristoyl phosphatidyl choline, natural phosphatidyl choline from eggs, natural phosphatidyl glycerol from eggs, cholesterol and isopropyl myristate.

[00474] Combinations of one or more erosion facilitator with one or more diffusion facilitator can also be used in the present compositions.

[00475] The term “diluent” refers to chemical compounds that are used to dilute the compound of interest prior to delivery. Diluents can also be used to stabilize compounds because they can provide a more stable environment. Salts dissolved in buffered solutions (which also can provide pH control or maintenance) are utilized as diluents in the art, including, but not limited to a phosphate buffered saline solution. In certain embodiments, diluents increase bulk of the composition to facilitate compression or create sufficient bulk for homogenous blend for capsule filling. Such compounds include e.g., lactose, starch, mannitol, sorbitol, dextrose, microcrystalline cellulose such as Avicel®; dibasic calcium phosphate, dicalcium phosphate dihydrate; tricalcium phosphate, calcium phosphate; anhydrous lactose, spray-dried lactose; pregelatinized starch, compressible sugar, such as Di-Pac® (Amstar); mannitol, hydroxypropylmethyl cellulose, hydroxypropylmethylcellulose acetate stearate, sucrose-based diluents, confectioner’s sugar; monobasic calcium sulfate monohydrate, calcium sulfate dihydrate; calcium lactate trihydrate, dextrates; hydrolyzed cereal solids, amylose; powdered cellulose, calcium carbonate; glycine, kaolin; mannitol, sodium chloride; inositol, bentonite, and the like.

[00476] The term “disintegrate” includes both the dissolution and dispersion of the dosage form when contacted with gastrointestinal fluid. “Disintegration agents or disintegrants” facilitate the breakup or disintegration of a substance. Examples of disintegration agents include a starch, e.g., a natural starch such as corn starch or potato starch, a pregelatinized starch such as National 1551 or Amijel®, or sodium starch glycolate such as Promogel® or Explotab®, a cellulose such as a wood product, methylcrystalline cellulose, e.g., Avicel®, Avicel® PH101, Avicel®PH102, Avicel® PH105, Elcema® P100, Emcocel®, Vivacel®, Ming Tia®, and Solka-Floc®, methylcellulose, croscarmellose, or a cross-linked cellulose, such as cross-linked sodium carboxymethylcellulose (Ac-Di-Sol®), crosslinked carboxymethylcellulose, or cross-linked croscarmellose, a cross-linked starch such as sodium starch glycolate, a cross-linked polymer such as crosspovidone, a cross-linked polyvinylpyrrolidone, alginate such as alginic acid or a salt of alginic acid such as sodium alginate, a clay such as Veegum® HV (magnesium aluminum silicate), a gum such as agar, guar, locust bean, Karaya, pectin, or tragacanth, sodium starch glycolate, bentonite, a natural sponge, a surfactant, a resin such as a cation-exchange resin, citrus pulp, sodium lauryl sulfate, sodium lauryl sulfate in combination starch, and the like.

[00477] “Drug absorption” or “absorption” typically refers to the process of movement of drug from site of administration of a drug across a barrier into a blood vessel or the site of action, e.g., a drug moving from the gastrointestinal tract into the portal vein or lymphatic system.

[00478] An “enteric coating” is a substance that remains substantially intact in the stomach but dissolves and releases the drug in the small intestine or colon. Generally, the enteric coating comprises a polymeric material that prevents release in the low pH environment of the stomach but that ionizes at a higher pH, typically a pH of 6 to 7, and thus dissolves sufficiently in the small intestine or colon to release the active agent therein.

[00479] “Erosion facilitators” include materials that control the erosion of a particular material in gastrointestinal fluid. Erosion facilitators are generally known to those of ordinary skill in the art. Exemplary erosion facilitators include, e.g., hydrophilic polymers, electrolytes, proteins, peptides, and amino acids.

[00480] “Filling agents” include compounds such as lactose, calcium carbonate, calcium phosphate, dibasic calcium phosphate, calcium sulfate, microcrystalline cellulose, cellulose powder, dextrose, dextrates, dextran, starches, pregelatinized starch, sucrose, xylitol, lactitol, mannitol, sorbitol, sodium chloride, polyethylene glycol, and the like.

[00481] “Flavoring agents” and/or “sweeteners” useful in the formulations described herein, include, e.g., acacia syrup, acesulfame K, alitame, anise, apple, aspartame, banana, Bavarian cream, berry, black currant, butterscotch, calcium citrate, camphor, caramel, cherry, cherry cream, chocolate, cinnamon, bubble gum, citrus, citrus punch, citrus cream, cotton candy, cocoa, cola, cool cherry, cool citrus, cyclamate, cylamate, dextrose, eucalyptus, eugenol, fructose, fruit punch, ginger, glycyrrhetinate, glycyrrhiza (licorice) syrup, grape, grapefruit, honey, isomalt, lemon, lime, lemon cream, monoammonium glyrrhizinate (MagnaSweet®), maltol, mannitol, maple, marshmallow, menthol, mint cream, mixed berry, neohesperidine DC, neotame, orange, pear, peach, peppermint, peppermint cream, Prosweet® Powder, raspberry, root beer, rum, saccharin, safrole, sorbitol, spearmint, spearmint cream, strawberry, strawberry cream, stevia, sucralose, sucrose, sodium saccharin, saccharin, aspartame, acesulfame potassium, mannitol, talin, sylitol, sucralose, sorbitol, Swiss cream, tagatose, tangerine, thaumatin, tutti fruitti, vanilla, walnut, watermelon, wild cherry, wintergreen, xylitol, or any combination of these flavoring ingredients, e.g., anise-menthol, cherryanise, cinnamon-orange, cherry-cinnamon, chocolate-mint, honey-lemon, lemon-lime, lemon-mint, menthol-eucalyptus, orange-cream, vanilla-mint, and mixtures thereof.

[00482] “Lubricants” and “glidants” are compounds that prevent, reduce or inhibit adhesion or friction of materials. Exemplary lubricants include, e.g., stearic acid, calcium hydroxide, talc, sodium stearyl fumerate, a hydrocarbon such as mineral oil, or hydrogenated vegetable oil such as hydrogenated soybean oil (Sterotex®), higher fatty acids and their alkali-metal and alkaline earth metal salts, such as aluminum, calcium, magnesium, zinc, stearic acid, sodium stearates, glycerol, talc, waxes, Stearowet®, boric acid, sodium benzoate, sodium acetate, sodium chloride, leucine, a polyethylene glycol (e.g., PEG-4000) or a methoxypolyethylene glycol such as Carbowax™, sodium oleate, sodium benzoate, glyceryl behenate, polyethylene glycol, magnesium or sodium lauryl sulfate, colloidal silica such as Syloid™, Cab-O-Sil®, a starch such as corn starch, silicone oil, a surfactant, and the like.

[00483] A “measurable serum concentration” or “measurable plasma concentration” describes the blood serum or blood plasma concentration, typically measured in mg, pg, or ng of therapeutic agent per ml, dl, or 1 of blood serum, absorbed into the bloodstream after administration. As used herein, measurable plasma concentrations are typically measured in ng/ml or pg/ml.

[00484] “Pharmacodynamics” refers to the factors which determine the biologic response observed relative to the concentration of drug at a site of action.

[00485] “Pharmacokinetics” refers to the factors which determine the attainment and maintenance of the appropriate concentration of drug at a site of action.

[00486] “Plasticizers” are compounds used to soften the microencapsulation material or film coatings to make them less brittle. Suitable plasticizers include, e.g., polyethylene glycols such as PEG 300, PEG 400, PEG 600, PEG 1450, PEG 3350, and PEG 800, stearic acid, propylene glycol, oleic acid, triethyl cellulose and triacetin. In some embodiments, plasticizers can also function as dispersing agents or wetting agents.

[00487] “Solubilizers” include compounds such as triacetin, tri ethyl citrate, ethyl oleate, ethyl caprylate, sodium lauryl sulfate, sodium doccusate, vitamin E TPGS, dimethylacetamide, N- methylpyrrolidone, N-hydroxyethylpyrrolidone, polyvinylpyrrolidone, hydroxypropylmethyl cellulose, hydroxypropyl cyclodextrins, ethanol, n-butanol, isopropyl alcohol, cholesterol, bile salts, polyethylene glycol 200-600, glycofurol, transcutol, propylene glycol, and dimethyl isosorbide and the like. [00488] “Stabilizers” include compounds such as any antioxidation agents, buffers, acids, preservatives and the like.

[00489] “Steady state,” as used herein, is when the amount of drug administered is equal to the amount of drug eliminated within one dosing interval resulting in a plateau or constant plasma drug exposure.

[00490] “Suspending agents” include compounds such as polyvinylpyrrolidone, e.g., polyvinylpyrrolidone K12, polyvinylpyrrolidone K17, polyvinylpyrrolidone K25, or polyvinylpyrrolidone K30, vinyl pyrrolidone/vinyl acetate copolymer (S630), polyethylene glycol, e.g., the polyethylene glycol can have a molecular weight of about 300 to about 6000, or about 3350 to about 4000, or about 7000 to about 5400, sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, hydroxymethylcellulose acetate stearate, polysorbate-80, hydroxyethylcellulose, sodium alginate, gums, such as, e.g., gum tragacanth and gum acacia, guar gum, xanthans, including xanthan gum, sugars, cellulosics, such as, e.g., sodium carboxymethylcellulose, methylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose, hydroxyethylcellulose, polysorbate-80, sodium alginate, polyethoxylated sorbitan monolaurate, polyethoxylated sorbitan monolaurate, povidone and the like. [00491] “Surfactants” include compounds such as sodium lauryl sulfate, sodium docusate, Tween 60 or 80, triacetin, vitamin E TPGS, sorbitan monooleate, polyoxyethylene sorbitan monooleate, polysorbates, polaxomers, bile salts, glyceryl monostearate, copolymers of ethylene oxide and propylene oxide, e.g., Pluronic® (BASF), and the like. Some other surfactants include polyoxyethylene fatty acid glycerides and vegetable oils, e.g., polyoxyethylene (60) hydrogenated castor oil; and polyoxyethylene alkylethers and alkylphenyl ethers, e.g., octoxynol 10, octoxynol 40. In some embodiments, surfactants may be included to enhance physical stability or for other purposes.

[00492] “Viscosity enhancing agents” include, e.g., methyl cellulose, xanthan gum, carboxymethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, hydroxypropylmethyl cellulose acetate stearate, hydroxypropylmethyl cellulose phthalate, carbomer, polyvinyl alcohol, alginates, acacia, chitosans and combinations thereof.

[00493] “Wetting agents” include compounds such as oleic acid, glyceryl monostearate, sorbitan monooleate, sorbitan monolaurate, triethanolamine oleate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan monolaurate, sodium docusate, sodium oleate, sodium lauryl sulfate, sodium doccusate, triacetin, Tween 80, vitamin E TPGS, ammonium salts and the like. Dosage Forms

[00494] The compositions described herein can be formulated for administration to a subject via any conventional means including, but not limited to, oral, parenteral (e.g., intravenous, subcutaneous, or intramuscular), buccal, intranasal, rectal or transdermal administration routes. As used herein, the term “subject” is used to mean an animal, preferably a mammal, including a human or non-human. The terms patient and subject may be used interchangeably.

[00495] Moreover, the pharmaceutical compositions described herein, which include a compound of any of Formula (L-I), (L-II), and (I) can be formulated into any suitable dosage form, including but not limited to, aqueous oral dispersions, liquids, gels, syrups, elixirs, slurries, suspensions and the like, for oral ingestion by a patient to be treated, solid oral dosage forms, aerosols, controlled release formulations, fast melt formulations, effervescent formulations, lyophilized formulations, tablets, powders, pills, dragees, capsules, delayed release formulations, extended release formulations, pulsatile release formulations, multiparticulate formulations, and mixed immediate release and controlled release formulations.

[00496] Pharmaceutical preparations for oral use can be obtained by mixing one or more solid excipient with one or more of the compounds described herein, optionally grinding the resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients include, for example, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methylcellulose, microcrystalline cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose; or others such as: polyvinylpyrrolidone (PVP or povidone) or calcium phosphate. If desired, disintegrating agents may be added, such as the cross-linked croscarmellose sodium, polyvinylpyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate.

[00497] Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may optionally contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.

[00498] Pharmaceutical preparations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers may be added. All formulations for oral administration should be in dosages suitable for such administration. [00499] In some embodiments, the solid dosage forms disclosed herein may be in the form of a tablet, (including a suspension tablet, a fast-melt tablet, a bite-disintegration tablet, a rapiddisintegration tablet, an effervescent tablet, or a caplet), a pill, a powder (including a sterile packaged powder, a dispensable powder, or an effervescent powder) a capsule (including both soft or hard capsules, e.g., capsules made from animal-derived gelatin or plant-derived HPMC, or “sprinkle capsules”), solid dispersion, solid solution, bioerodible dosage form, controlled release formulations, pulsatile release dosage forms, multiparticulate dosage forms, pellets, granules, or an aerosol. In some embodiments, the pharmaceutical composition is in the form of a powder. In some embodiments, the pharmaceutical composition is in the form of a tablet, including but not limited to, a fast-melt tablet. Additionally, pharmaceutical compositions described herein may be administered as a single capsule or in multiple capsule dosage form. In some embodiments, the pharmaceutical composition is administered in two, or three, or four, capsules or tablets.

[00500] In some embodiments, solid dosage forms, e.g., tablets, effervescent tablets, and capsules, are prepared by mixing particles of a compound of any of Formula (L-I), (L-II), and (I) with one or more pharmaceutical excipients to form a bulk blend composition. When referring to these bulk blend compositions as homogeneous, it is meant that the particles of the compound of any of Formula (L-I), (L-II), and (I) are dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms, such as tablets, pills, and capsules. The individual unit dosages may also include film coatings, which disintegrate upon oral ingestion or upon contact with diluent. These formulations can be manufactured by conventional pharmacological techniques.

[00501] Conventional pharmacological techniques include, e.g., one or a combination of methods: (1) dry mixing, (2) direct compression, (3) milling, (4) dry or non-aqueous granulation, (5) wet granulation, or (6) fusion. See, e.g., Lachman et al., The Theory and Practice of Industrial Pharmacy (1986). Other methods include, e.g., spray drying, pan coating, melt granulation, granulation, fluidized bed spray drying or coating (e.g., wurster coating), tangential coating, top spraying, tableting, extruding and the like. [00502] The pharmaceutical solid dosage forms described herein can include a compound described herein and one or more pharmaceutically acceptable additives such as a compatible carrier, binder, filling agent, suspending agent, flavoring agent, sweetening agent, disintegrating agent, dispersing agent, surfactant, lubricant, colorant, diluent, solubilizer, moistening agent, plasticizer, stabilizer, penetration enhancer, wetting agent, anti-foaming agent, antioxidant, preservative, or one or more combination thereof. In some embodiments, using standard coating procedures, such as those described in Remington’s Pharmaceutical Sciences, 20th Edition (2000), a film coating is provided around the formulation of the compound of any of Formula (L-I), (L-II), and (I). In some embodiments, some or all of the particles of the compound of any of Formula (L-I), (L-II), and (I) are coated. In some embodiments, some or all of the particles of the compound of any of Formula (L-I), (L-II), and (I), are microencapsulated. In still some embodiments, the particles of the compound of any of Formula (L-I), (L-II), and (I) are not microencapsulated and are uncoated. [00503] Suitable carriers for use in the solid dosage forms described herein include, but are not limited to, acacia, gelatin, colloidal silicon dioxide, calcium glycerophosphate, calcium lactate, maltodextrin, glycerine, magnesium silicate, sodium caseinate, soy lecithin, sodium chloride, tricalcium phosphate, dipotassium phosphate, sodium stearoyl lactylate, carrageenan, monoglyceride, diglyceride, pregelatinized starch, hydroxypropylmethylcellulose, hydroxypropylmethylcellulose acetate stearate, sucrose, microcrystalline cellulose, lactose, mannitol and the like.

[00504] Suitable filling agents for use in the solid dosage forms described herein include, but are not limited to, lactose, calcium carbonate, calcium phosphate, dibasic calcium phosphate, calcium sulfate, microcrystalline cellulose, cellulose powder, dextrose, dextrates, dextran, starches, pregelatinized starch, hydroxypropylmethycellulose (HPMC), hydroxypropylmethycellulose phthalate, hydroxypropylmethylcellulose acetate stearate (HPMCAS), sucrose, xylitol, lactitol, mannitol, sorbitol, sodium chloride, polyethylene glycol, and the like.

[00505] In order to release the compound of any of Formula (L-I), (L-II), and (I) from a solid dosage form matrix as efficiently as possible, disintegrants are often used in the formulation, especially when the dosage forms are compressed with binder. Disintegrants help rupturing the dosage form matrix by swelling or capillary action when moisture is absorbed into the dosage form. Suitable disintegrants for use in the solid dosage forms described herein include, but are not limited to, natural starch such as corn starch or potato starch, a pregelatinized starch such as National 1551 or Amijel®, or sodium starch glycolate such as Promogel® or Explotab®, a cellulose such as a wood product, methylcrystalline cellulose, e.g., Avicel®, Avicel® PH101, Avicel®PH102, Avicel® PH105, Elcema® P100, Emcocel®, Vivacel®, Ming Tia®, and Solka-Floc®, methylcellulose, croscarmellose, or a cross-linked cellulose, such as cross-linked sodium carboxymethylcellulose (Ac-Di-Sol®), crosslinked carboxymethylcellulose, or cross-linked croscarmellose, a cross-linked starch such as sodium starch glycolate, a cross-linked polymer such as crospovidone, a cross-linked polyvinylpyrrolidone, alginate such as alginic acid or a salt of alginic acid such as sodium alginate, a clay such as Veegum® HV (magnesium aluminum silicate), a gum such as agar, guar, locust bean, Karaya, pectin, or tragacanth, sodium starch glycolate, bentonite, a natural sponge, a surfactant, a resin such as a cation-exchange resin, citrus pulp, sodium lauryl sulfate, sodium lauryl sulfate in combination starch, and the like.

[00506] Binders impart cohesiveness to solid oral dosage form formulations: for powder filled capsule formulation, they aid in plug formation that can be filled into soft or hard shell capsules and for tablet formulation, they ensure the tablet remaining intact after compression and help assure blend uniformity prior to a compression or fill step. Materials suitable for use as binders in the solid dosage forms described herein include, but are not limited to, carboxymethylcellulose, methylcellulose (e.g., Methocel®), hydroxypropylmethylcellulose (e.g. Hypromellose USP Pharmacoat-603, hydroxypropylmethylcellulose acetate stearate (Aqoate HS-LF and HS), hydroxyethylcellulose, hydroxypropyl cellulose (e.g., Klucel®), ethylcellulose (e.g., Ethocel®), and microcrystalline cellulose (e.g., Avicel®), microcrystalline dextrose, amylose, magnesium aluminum silicate, polysaccharide acids, bentonites, gelatin, polyvinylpyrrolidone/vinyl acetate copolymer, crospovidone, povidone, starch, pregelatinized starch, tragacanth, dextrin, a sugar, such as sucrose (e.g., Dipac®), glucose, dextrose, molasses, mannitol, sorbitol, xylitol (e.g., Xylitab®), lactose, a natural or synthetic gum such as acacia, tragacanth, ghatti gum, mucilage of isapol husks, starch, polyvinylpyrrolidone (e.g., Povidone® CL, Kollidon® CL, Polyplasdone® XL-10, and Povidone® K- 12), larch arabogalactan, Veegum®, polyethylene glycol, waxes, sodium alginate, and the like. [00507] In general, binder levels of 20-70% are used in powder-filled gelatin capsule formulations. Binder usage level in tablet formulations varies whether direct compression, wet granulation, roller compaction, or usage of other excipients such as fillers which itself can act as moderate binder. Formulators skilled in art can determine the binder level for the formulations, but binder usage level of up to 70% in tablet formulations is common.

[00508] Suitable lubricants or glidants for use in the solid dosage forms described herein include, but are not limited to, stearic acid, calcium hydroxide, talc, com starch, sodium stearyl fumerate, alkali- metal and alkaline earth metal salts, such as aluminum, calcium, magnesium, zinc, stearic acid, sodium stearates, magnesium stearate, zinc stearate, waxes, Stearowet®, boric acid, sodium benzoate, sodium acetate, sodium chloride, leucine, a polyethylene glycol or a methoxypolyethylene glycol such as Carbowax™, PEG 4000, PEG 5000, PEG 6000, propylene glycol, sodium oleate, glyceryl behenate, glyceryl palmitostearate, glyceryl benzoate, magnesium or sodium lauryl sulfate, and the like.

[00509] Suitable diluents for use in the solid dosage forms described herein include, but are not limited to, sugars (including lactose, sucrose, and dextrose), polysaccharides (including dextrates and maltodextrin), polyols (including mannitol, xylitol, and sorbitol), cyclodextrins and the like. [00510] The term “non water-soluble diluent” represents compounds typically used in the formulation of pharmaceuticals, such as calcium phosphate, calcium sulfate, starches, modified starches and microcrystalline cellulose, and microcellulose (e.g., having a density of about 0.45 g/cm ³ , e.g. Avicel, powdered cellulose), and talc.

[00511] Suitable wetting agents for use in the solid dosage forms described herein include, for example, oleic acid, glyceryl monostearate, sorbitan monooleate, sorbitan monolaurate, triethanolamine oleate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan monolaurate, quaternary ammonium compounds (e.g., Polyquat 10®), sodium oleate, sodium lauryl sulfate, magnesium stearate, sodium docusate, triacetin, vitamin E TPGS and the like.

[00512] Suitable surfactants for use in the solid dosage forms described herein include, for example, sodium lauryl sulfate, sorbitan monooleate, polyoxyethylene sorbitan monooleate, polysorbates, polaxomers, bile salts, glyceryl monostearate, copolymers of ethylene oxide and propylene oxide, e.g., Pluronic® (BASF), and the like.

[00513] Suitable suspending agents for use in the solid dosage forms described here include, but are not limited to, polyvinylpyrrolidone, e.g., polyvinylpyrrolidone K12, polyvinylpyrrolidone K17, polyvinylpyrrolidone K25, or polyvinylpyrrolidone K30, polyethylene glycol, e.g., the polyethylene glycol can have a molecular weight of about 300 to about 6000, or about 3350 to about 4000, or about 7000 to about 5400, vinyl pyrrolidone/vinyl acetate copolymer (S630), sodium carboxymethylcellulose, methylcellulose, hydroxy-propylmethylcellulose, polysorbate-80, hydroxyethylcellulose, sodium alginate, gums, such as, e.g., gum tragacanth and gum acacia, guar gum, xanthans, including xanthan gum, sugars, cellulosics, such as, e.g., sodium carboxymethylcellulose, methylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose, hydroxyethylcellulose, polysorbate-80, sodium alginate, polyethoxylated sorbitan monolaurate, polyethoxylated sorbitan monolaurate, povidone and the like. [00514] Suitable antioxidants for use in the solid dosage forms described herein include, for example, e.g., butylated hydroxytoluene (BHT), sodium ascorbate, and tocopherol.

[00515] It should be appreciated that there is considerable overlap between additives used in the solid dosage forms described herein. Thus, the above-listed additives should be taken as merely exemplary, and not limiting, of the types of additives that can be included in solid dosage forms described herein. The amounts of such additives can be readily determined by one skilled in the art, according to the particular properties desired.

[00516] In some embodiments, one or more layers of the pharmaceutical composition are plasticized. Illustratively, a plasticizer is generally a high boiling point solid or liquid. Suitable plasticizers can be added from about 0.01% to about 50% by weight (w/w) of the coating composition. Plasticizers include, but are not limited to, diethyl phthalate, citrate esters, polyethylene glycol, glycerol, acetylated glycerides, triacetin, polypropylene glycol, polyethylene glycol, triethyl citrate, dibutyl sebacate, stearic acid, stearol, stearate, and castor oil.

[00517] Compressed tablets are solid dosage forms prepared by compacting the bulk blend of the formulations described above. In various embodiments, compressed tablets which are designed to dissolve in the mouth will include one or more flavoring agents. In some embodiments, the compressed tablets will include a film surrounding the final compressed tablet. In some embodiments, the film coating can provide a delayed release of the compound of of any of Formula (L-I), (L-II), and (I) from the formulation. In some embodiments, the film coating aids in patient compliance (e.g., Opadry® coatings or sugar coating). Film coatings including Opadry® typically range from about 1% to about 3% of the tablet weight. In some embodiments, the compressed tablets include one or more excipients.

[00518] A capsule may be prepared, for example, by placing the bulk blend of the formulation of the compound of any of Formula (L-I), (L-II), and (I), described above, inside of a capsule. In some embodiments, the formulations (non-aqueous suspensions and solutions) are placed in a soft gelatin capsule. In some embodiments, the formulations are placed in standard gelatin capsules or nongelatin capsules such as capsules comprising HPMC. In some embodiments, the formulation is placed in a sprinkle capsule, wherein the capsule may be swallowed whole or the capsule may be opened and the contents sprinkled on food prior to eating. In some embodiments, the therapeutic dose is split into multiple (e.g., two, three, or four) capsules. In some embodiments, the entire dose of the formulation is delivered in a capsule form.

[00519] In various embodiments, the particles of the compound of any of Formula (L-I), (L-II), and (I) and one or more excipients are dry blended and compressed into a mass, such as a tablet, having a hardness sufficient to provide a pharmaceutical composition that substantially disintegrates within less than about 30 minutes, less than about 35 minutes, less than about 40 minutes, less than about 45 minutes, less than about 50 minutes, less than about 55 minutes, or less than about 60 minutes, after oral administration, thereby releasing the formulation into the gastrointestinal fluid.

[00520] In some embodiments, dosage forms may include microencapsulated formulations. In some embodiments, one or more other compatible materials are present in the microencapsulation material. Exemplary materials include, but are not limited to, pH modifiers, erosion facilitators, antifoaming agents, antioxidants, flavoring agents, and carrier materials such as binders, suspending agents, disintegration agents, filling agents, surfactants, solubilizers, stabilizers, lubricants, wetting agents, and diluents.

[00521] Materials useful for the microencapsulation described herein include materials compatible with compounds of any of Formula (L-I), (L-II), and (I) which sufficiently isolate the compound of any of Formula (L-I), (L-II), and (I) from other non-compatible excipients. Materials compatible with compounds of any of Formula (L-I), (L-II), and (I) are those that delay the release of the compounds of of any of Formula (L-I), (L-II), and (I), in vivo.

[00522] Exemplary microencapsulation materials useful for delaying the release of the formulations including compounds described herein, include, but are not limited to, hydroxypropyl cellulose ethers (HPC) such as Klucel® or Nisso HPC, low-substituted hydroxypropyl cellulose ethers (L- HPC), hydroxypropyl methyl cellulose ethers (HPMC) such as Seppifilm-LC, Pharmacoat®, Metolose SR, Methocel ®-E, Opadry YS, PrimaFlo, Benecel MP824, and Benecel MP843, methylcellulose polymers such as Methocel®-A, hydroxypropylmethylcellulose acetate stearate Aqoat (HF-LS, HF-LG,HF-MS) and Metolose®, Ethylcelluloses (EC) and mixtures thereof such as E461, Ethocel®, Aquaion ®-EC, Surelease®, Polyvinyl alcohol (PVA) such as Opadry AMB, hydroxyethylcelluloses such as Natrosol®, carboxymethylcelluloses and salts of carboxymethylcelluloses (CMC) such as Aqualon®-CMC, polyvinyl alcohol and polyethylene glycol co-polymers such as Kollicoat IR®, monoglycerides (Myverol), triglycerides (KLX), polyethylene glycols, modified food starch, acrylic polymers and mixtures of acrylic polymers with cellulose ethers such as Eudragit® EPO, Eudragit® L30D-55, Eudragit® FS 30D Eudragit® L100-55, Eudragit® L100, Eudragit® S100, Eudragit® RD100, Eudragit® E100, Eudragit® L12.5, Eudragit® S12.5, Eudragit® NE30D, and Eudragit® NE 40D, cellulose acetate phthalate, sepifilms such as mixtures of HPMC and stearic acid, cyclodextrins, and mixtures of these materials.

[00523] In some embodiments, plasticizers such as polyethylene glycols, e.g., PEG 300, PEG 400, PEG 600, PEG 1450, PEG 3350, and PEG 800, stearic acid, propylene glycol, oleic acid, and triacetin are incorporated into the microencapsulation material. In some embodiments, the microencapsulating material useful for delaying the release of the pharmaceutical compositions is from the USP or the National Formulary (NF). In some embodiments, the microencapsulation material is Klucel. In some embodiments, the microencapsulation material is methocel.

[00524] Microencapsulated compounds of any of Formula (L-I), (L-II), and (I) may be formulated by methods known by one of ordinary skill in the art. Such known methods include, e.g., spray drying processes, spinning disk-solvent processes, hot melt processes, spray chilling methods, fluidized bed, electrostatic deposition, centrifugal extrusion, rotational suspension separation, polymerization at liquid-gas or solid-gas interface, pressure extrusion, or spraying solvent extraction bath. In addition to these, several chemical techniques, e.g., complex coacervation, solvent evaporation, polymer-polymer incompatibility, interfacial polymerization in liquid media, in situ polymerization, in-liquid drying, and desolvation in liquid media could also be used. Furthermore, other methods such as roller compaction, extrusion/spheronization, coacervation, or nanoparticle coating may also be used.

[00525] In some embodiments, the particles of compounds of any of Formula (L-I), (L-II), and (I) are microencapsulated prior to being formulated into one of the above forms. In still some embodiments, some or most of the particles are coated prior to being further formulated by using standard coating procedures, such as those described in Remington’s Pharmaceutical Sciences, 20th Edition (2000).

[00526] In some embodiments, the solid dosage formulations of the compounds of any of Formula (L-I), (L-II), and (I) are plasticized (coated) with one or more layers. Illustratively, a plasticizer is generally a high boiling point solid or liquid. Suitable plasticizers can be added from about 0.01% to about 50% by weight (w/w) of the coating composition. Plasticizers include, but are not limited to, diethyl phthalate, citrate esters, polyethylene glycol, glycerol, acetylated glycerides, triacetin, polypropylene glycol, polyethylene glycol, triethyl citrate, dibutyl sebacate, stearic acid, stearol, stearate, and castor oil. [00527] In some embodiments, a powder including the formulations with a compound of any of Formula (I)-(XIc), described herein, may be formulated to include one or more pharmaceutical excipients and flavors. Such a powder may be prepared, for example, by mixing the formulation and optional pharmaceutical excipients to form a bulk blend composition. Additional embodiments also include a suspending agent and/or a wetting agent. This bulk blend is uniformly subdivided into unit dosage packaging or multi-dosage packaging units.

[00528] In still some embodiments, effervescent powders are also prepared in accordance with the present disclosure. Effervescent salts have been used to disperse medicines in water for oral administration. Effervescent salts are granules or coarse powders containing a medicinal agent in a dry mixture, usually composed of sodium bicarbonate, citric acid, and/or tartaric acid. When salts of the compositions described herein are added to water, the acids and the base react to liberate carbon dioxide gas, thereby causing “effervescence.” Examples of effervescent salts include, e.g., the following ingredients: sodium bicarbonate or a mixture of sodium bicarbonate and sodium carbonate, citric acid and/or tartaric acid. Any acid-base combination that results in the liberation of carbon dioxide can be used in place of the combination of sodium bicarbonate and citric and tartaric acids, as long as the ingredients were suitable for pharmaceutical use and result in a pH of about 6.0 or higher.

[00529] In some embodiments, the formulations described herein, which include a compound of Formula (I), are solid dispersions. Methods of producing such solid dispersions are known in the art and include, but are not limited to, for example, U.S. Pat. Nos. 4,343,789, 5,340,591, 5,456,923, 5,700,485, 5,723,269, and U.S. Pub. Appl 2004/0013734, each of which is specifically incorporated by reference. In some embodiments, the formulations described herein are solid solutions. Solid solutions incorporate a substance together with the active agent and other excipients such that heating the mixture results in dissolution of the drug and the resulting composition is then cooled to provide a solid blend which can be further formulated or directly added to a capsule or compressed into a tablet. Methods of producing such solid solutions are known in the art and include, but are not limited to, for example, U.S. Pat. Nos. 4,151,273, 5,281,420, and 6,083,518, each of which is specifically incorporated by reference.

[00530] The pharmaceutical solid oral dosage forms including formulations described herein, which include a compound of any of Formula (L-I), (L-II), and (I) can be further formulated to provide a controlled release of the compound of Formula (I). Controlled release refers to the release of the compound of any of Formula (L-I), (L-II), and (I) from a dosage form in which it is incorporated according to a desired profile over an extended period of time. Controlled release profiles include, for example, sustained release, prolonged release, pulsatile release, and delayed release profiles. In contrast to immediate release compositions, controlled release compositions allow delivery of an agent to a subject over an extended period of time according to a predetermined profile. Such release rates can provide therapeutically effective levels of agent for an extended period of time and thereby provide a longer period of pharmacologic response while minimizing side effects as compared to conventional rapid release dosage forms. Such longer periods of response provide for many inherent benefits that are not achieved with the corresponding short acting, immediate release preparations. [00531] In some embodiments, the solid dosage forms described herein can be formulated as enteric coated delayed release oral dosage forms, i.e., as an oral dosage form of a pharmaceutical composition as described herein which utilizes an enteric coating to affect release in the small intestine of the gastrointestinal tract. The enteric coated dosage form may be a compressed or molded or extruded tablet/mold (coated or uncoated) containing granules, powder, pellets, beads or particles of the active ingredient and/or other composition components, which are themselves coated or uncoated. The enteric coated oral dosage form may also be a capsule (coated or uncoated) containing pellets, beads or granules of the solid carrier or the composition, which are themselves coated or uncoated.

[00532] The term “delayed release” as used herein refers to the delivery so that the release can be accomplished at some generally predictable location in the intestinal tract more distal to that which would have been accomplished if there had been no delayed release alterations. In some embodiments the method for delay of release is coating. Any coatings should be applied to a sufficient thickness such that the entire coating does not dissolve in the gastrointestinal fluids at pH below about 5, but does dissolve at pH about 5 and above. It is expected that any anionic polymer exhibiting a pH-dependent solubility profile can be used as an enteric coating in the methods and compositions described herein to achieve delivery to the lower gastrointestinal tract. In some embodiments the polymers described herein are anionic carboxylic polymers. In some embodiments, the polymers and compatible mixtures thereof, and some of their properties, include, but are not limited to:

[00533] Shellac, also called purified lac, a refined product obtained from the resinous secretion of an insect. This coating dissolves in media of pH >7;

[00534] Acrylic polymers. The performance of acrylic polymers (primarily their solubility in biological fluids) can vary based on the degree and type of substitution. Examples of suitable acrylic polymers include methacrylic acid copolymers and ammonium methacrylate copolymers. The Eudragit series E, L, S, RL, RS, and NE (Rohm Pharma) are available as solubilized in organic solvent, aqueous dispersion, or dry powders. The Eudragit series RL, NE, and RS are insoluble in the gastrointestinal tract but are permeable and are used primarily for colonic targeting. The Eudragit series E dissolve in the stomach. The Eudragit series L, L-30D and S are insoluble in stomach and dissolve in the intestine;

[00535] Cellulose Derivatives. Examples of suitable cellulose derivatives are: ethyl cellulose; reaction mixtures of partial acetate esters of cellulose with phthalic anhydride. The performance can vary based on the degree and type of substitution. Cellulose acetate phthalate (CAP) dissolves in pH >6. Aquateric (FMC) is an aqueous based system and is a spray dried CAP psuedolatex with particles <1 pm. Other components in Aquateric can include pluronics, Tweens, and acetylated monoglycerides. Other suitable cellulose derivatives include: cellulose acetate trimellitate (Eastman); methylcellulose (Pharmacoat, Methocel); hydroxypropylmethyl cellulose phthalate (HPMCP); hydroxypropylmethyl cellulose succinate (HPMCS); and hydroxypropylmethylcellulose acetate succinate (e.g., AQOAT (Shin Etsu)). The performance can vary based on the degree and type of substitution. For example, HPMCP such as, HP-50, HP-55, HP-55S, HP-55F grades are suitable. The performance can vary based on the degree and type of substitution. For example, suitable grades of hydroxypropylmethylcellulose acetate succinate include, but are not limited to, AS-LG (LF), which dissolves at pH 5, AS-MG (MF), which dissolves at pH 5.5, and AS-HG (HF), which dissolves at higher pH. These polymers are offered as granules, or as fine powders for aqueous dispersions;

[00536] Poly Vinyl Acetate Phthalate (PVAP). PVAP dissolves in pH >5, and it is much less permeable to water vapor and gastric fluids.

[00537] In some embodiments, the coating can, and usually does, contain a plasticizer and possibly other coating excipients such as colorants, talc, and/or magnesium stearate, which are well known in the art. Suitable plasticizers include triethyl citrate (Citroflex 2), triacetin (glyceryl triacetate), acetyl triethyl citrate (Citroflec A2), Carbowax 400 (polyethylene glycol 400), diethyl phthalate, tributyl citrate, acetylated monoglycerides, glycerol, fatty acid esters, propylene glycol, and dibutyl phthalate. In particular, anionic carboxylic acrylic polymers usually will contain 10-25% by weight of a plasticizer, especially dibutyl phthalate, polyethylene glycol, triethyl citrate, and triacetin. Conventional coating techniques such as spray or pan coating are employed to apply coatings. The

- Ill - coating thickness must be sufficient to ensure that the oral dosage form remains intact until the desired site of topical delivery in the intestinal tract is reached.

100538] Colorants, detackifiers, surfactants, antifoaming agents, lubricants (e.g., carnuba wax or PEG) may be added to the coatings besides plasticizers to solubilize or disperse the coating material, and to improve coating performance and the coated product.

[00539] In some embodiments, the formulations described herein, which include a compound of Formula (I), are delivered using a pulsatile dosage form. A pulsatile dosage form is capable of providing one or more immediate release pulses at predetermined time points after a controlled lag time or at specific sites. Pulsatile dosage forms including the formulations described herein, which include a compound of any of Formula (L-I), (L-II), and (I) may be administered using a variety of pulsatile formulations known in the art. For example, such formulations include, but are not limited to, those described in U.S. Pat. Nos. 5,011,692, 5,017,381, 5,229,135, and 5,840,329, each of which is specifically incorporated by reference. Other pulsatile release dosage forms suitable for use with the present formulations include, but are not limited to, for example, U.S. Pat. Nos. 4,871,549, 5,260,068, 5,260,069, 5,508,040, 5,567,441 and 5,837,284, all of which are specifically incorporated by reference. In some embodiments, the controlled release dosage form is pulsatile release solid oral dosage form including at least two groups of particles, (i.e. multiparticulate) each containing the formulation described herein. The first group of particles provides a substantially immediate dose of the compound of any of Formula (L-I), (L-II), and (I) upon ingestion by a mammal. The first group of particles can be either uncoated or include a coating and/or sealant. The second group of particles includes coated particles, which includes from about 2% to about 75%, from about 2.5% to about 70%, or from about 40% to about 70%, by weight of the total dose of the compound of any of Formula (L-I), (L-II), and (I) in said formulation, in admixture with one or more binders. The coating includes a pharmaceutically acceptable ingredient in an amount sufficient to provide a delay of from about 2 hours to about 7 hours following ingestion before release of the second dose.

Suitable coatings include one or more differentially degradable coatings such as, by way of example only, pH sensitive coatings (enteric coatings) such as acrylic resins (e.g., Eudragit® EPO, Eudragit® L30D-55, Eudragit® FS 30D Eudragit® L100-55, Eudragit® L100, Eudragit® S100, Eudragit® RD 100, Eudragit® El 00, Eudragit® LI 2.5, Eudragit® S12.5, and Eudragit® NE30D, Eudragit® NE 40D® ) either alone or blended with cellulose derivatives, e.g., ethylcellulose, or non-enteric coatings having variable thickness to provide differential release of the formulation that includes a compound of any of Formula (I). [00540] Many other types of controlled release systems known to those of ordinary skill in the art and are suitable for use with the formulations described herein. Examples of such delivery systems include, e.g., polymer-based systems, such as polylactic and polyglycolic acid, plyanhydrides and polycaprolactone; porous matrices, nonpolymer-based systems that are lipids, including sterols, such as cholesterol, cholesterol esters and fatty acids, or neutral fats, such as mono-, di- and triglycerides; hydrogel release systems; silastic systems; peptide-based systems; wax coatings, bioerodible dosage forms, compressed tablets using conventional binders and the like. See, e.g., Liberman et al., Pharmaceutical Dosage Forms, 2 Ed., Vol. 1, pp. 209-214 (1990); Singh et al., Encyclopedia of Pharmaceutical Technology, 2 ^nd Ed., pp. 751-753 (2002); U.S. Pat. Nos. 4,327,725, 4,624,848, 4,968,509, 5,461,140, 5,456,923, 5,516,527, 5,622,721, 5,686,105, 5,700,410, 5,977,175, 6,465,014, and 6,932,983, each of which is specifically incorporated by reference.

[00541] In some embodiments, pharmaceutical compositions are provided that include particles of the compounds of any of Formula (L-I), (L-II), and (I), described herein and at least one dispersing agent or suspending agent for oral administration to a subject. The formulations may be a powder and/or granules for suspension, and upon admixture with water, a substantially uniform suspension is obtained.

[00542] Liquid formulation dosage forms for oral administration can be aqueous suspensions selected from the group including, but not limited to, pharmaceutically acceptable aqueous oral dispersions, emulsions, solutions, elixirs, gels, and syrups. See, e.g., Singh et al., Encyclopedia of Pharmaceutical Technology, 2 ^nd Ed., pp. 754-757 (2002). In addition to the particles of compound of Formula (I), the liquid dosage forms may include additives, such as: (a) disintegrating agents; (b) dispersing agents; (c) wetting agents; (d) at least one preservative, (e) viscosity enhancing agents, (f) at least one sweetening agent, and (g) at least one flavoring agent. In some embodiments, the aqueous dispersions can further include a crystalline inhibitor.

[00543] The aqueous suspensions and dispersions described herein can remain in a homogenous state, as defined in The USP Pharmacists’ Pharmacopeia (2005 edition, chapter 905), for at least 4 hours. The homogeneity should be determined by a sampling method consistent with regard to determining homogeneity of the entire composition. In some embodiments, an aqueous suspension can be re-suspended into a homogenous suspension by physical agitation lasting less than 1 minute. In some embodiments, an aqueous suspension can be re-suspended into a homogenous suspension by physical agitation lasting less than 45 seconds. In yet some embodiments, an aqueous suspension can be re-suspended into a homogenous suspension by physical agitation lasting less than 30 seconds. In still some embodiments, no agitation is necessary to maintain a homogeneous aqueous dispersion.

[00544] Examples of disintegrating agents for use in the aqueous suspensions and dispersions include, but are not limited to, a starch, e.g., a natural starch such as com starch or potato starch, a pregelatinized starch such as National 1551 or Amijel®, or sodium starch glycolate such as Promogel® or Explotab®; a cellulose such as a wood product, methylcrystalline cellulose, e.g., Avicel®, Avicel® PH101, Avicel®PH102, Avicel® PH105, Elcema® P100, Emcocel®, Vivacel®, Ming Tia®, and Solka-Floc®, methylcellulose, croscarmellose, or a cross-linked cellulose, such as cross-linked sodium carboxymethylcellulose (Ac-Di-Sol®), cross-linked carboxymethylcellulose, or cross-linked croscarmellose; a cross-linked starch such as sodium starch glycolate; a cross-linked polymer such as crospovidone; a cross-linked polyvinylpyrrolidone; alginate such as alginic acid or a salt of alginic acid such as sodium alginate; a clay such as Veegum® HV (magnesium aluminum silicate); a gum such as agar, guar, locust bean, Karaya, pectin, or tragacanth; sodium starch glycolate; bentonite; a natural sponge; a surfactant; a resin such as a cation-exchange resin; citrus pulp; sodium lauryl sulfate; sodium lauryl sulfate in combination starch; and the like.

[00545] In some embodiments, the dispersing agents suitable for the aqueous suspensions and dispersions described herein are known in the art and include, for example, hydrophilic polymers, electrolytes, Tween ® 60 or 80, PEG, polyvinylpyrrolidone (PVP; commercially known as Plasdone®), and the carbohydrate-based dispersing agents such as, for example, hydroxypropyl cellulose and hydroxypropyl cellulose ethers (e.g., HPC, HPC-SL, and HPC-L), hydroxypropyl methylcellulose and hydroxypropyl methylcellulose ethers (e.g. HPMC K100, HPMC K4M, HPMC K15M, and HPMC KI OOM), carboxymethylcellulose sodium, methylcellulose, hydroxyethylcellulose, hydroxypropylmethyl-cellulose phthalate, hydroxypropylmethyl-cellulose acetate stearate, noncrystalline cellulose, magnesium aluminum silicate, triethanolamine, polyvinyl alcohol (PVA), polyvinylpyrrolidone/vinyl acetate copolymer (Plasdone®, e.g., S-630), 4-(l, 1,3,3- tetramethylbutyl)-phenol polymer with ethylene oxide and formaldehyde (also known as tyloxapol), poloxamers (e.g., Pluronics F68®, F88®, and F108®, which are block copolymers of ethylene oxide and propylene oxide); and poloxamines (e.g., Tetronic 908®, also known as Poloxamine 908®, which is a tetrafunctional block copolymer derived from sequential addition of propylene oxide and ethylene oxide to ethylenediamine (BASF Corporation, Parsippany, N.J.)). In some embodiments, the dispersing agent is selected from a group not comprising one of the following agents: hydrophilic polymers; electrolytes; Tween ® 60 or 80; PEG; polyvinylpyrrolidone (PVP); hydroxypropyl cellulose and hydroxypropyl cellulose ethers (e.g., HPC, HPC-SL, and HPC-L); hydroxypropyl methylcellulose and hydroxypropyl methylcellulose ethers (e.g. HPMC K100, HPMC K4M, HPMC K15M, HPMC KI OOM, and Pharmacoat® USP 2910 (Shin-Etsu)); carboxymethylcellulose sodium; methylcellulose; hydroxyethyl cellulose; hydroxypropylmethylcellulose phthalate; hydroxypropylmethyl-cellulose acetate stearate; non-crystalline cellulose; magnesium aluminum silicate; triethanolamine; polyvinyl alcohol (PVA); 4-(l, 1,3,3- tetramethylbutyl)-phenol polymer with ethylene oxide and formaldehyde; poloxamers (e.g., Pluronics F68®, F88®, and F108®, which are block copolymers of ethylene oxide and propylene oxide); or poloxamines (e.g., Tetronic 908®, also known as Poloxamine 908®).

[00546] Wetting agents suitable for the aqueous suspensions and dispersions described herein are known in the art and include, but are not limited to, cetyl alcohol, glycerol monostearate, polyoxyethylene sorbitan fatty acid esters (e.g., the commercially available Tweens® such as e.g., Tween 20® and Tween 80® (ICI Specialty Chemicals)), and polyethylene glycols (e.g., Carbowaxs 3350® and 1450®, and Carbopol 934® (Union Carbide)), oleic acid, glyceryl monostearate, sorbitan monooleate, sorbitan monolaurate, triethanolamine oleate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan monolaurate, sodium oleate, sodium lauryl sulfate, sodium docusate, triacetin, vitamin E TPGS, sodium taurocholate, simethicone, phosphotidylcholine and the like [00547] Suitable preservatives for the aqueous suspensions or dispersions described herein include, for example, potassium sorbate, parabens (e.g., methylparaben and propylparaben), benzoic acid and its salts, other esters of parahydroxybenzoic acid such as butylparaben, alcohols such as ethyl alcohol or benzyl alcohol, phenolic compounds such as phenol, or quaternary compounds such as benzalkonium chloride. Preservatives, as used herein, are incorporated into the dosage form at a concentration sufficient to inhibit microbial growth.

[00548] Suitable viscosity enhancing agents for the aqueous suspensions or dispersions described herein include, but are not limited to, methyl cellulose, xanthan gum, carboxymethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, Plasdon® S-630, carbomer, polyvinyl alcohol, alginates, acacia, chitosans and combinations thereof. The concentration of the viscosity enhancing agent will depend upon the agent selected and the viscosity desired.

[00549] Examples of sweetening agents suitable for the aqueous suspensions or dispersions described herein include, for example, acacia syrup, acesulfame K, alitame, anise, apple, aspartame, banana, Bavarian cream, berry, black currant, butterscotch, calcium citrate, camphor, caramel, cherry, cherry cream, chocolate, cinnamon, bubble gum, citrus, citrus punch, citrus cream, cotton candy, cocoa, cola, cool cherry, cool citrus, cyclamate, cylamate, dextrose, eucalyptus, eugenol, fructose, fruit punch, ginger, glycyrrhetinate, glycyrrhiza (licorice) syrup, grape, grapefruit, honey, isomalt, lemon, lime, lemon cream, monoammonium glyrrhizinate (Magna Sweet®), maltol, mannitol, maple, marshmallow, menthol, mint cream, mixed berry, neohesperidine DC, neotame, orange, pear, peach, peppermint, peppermint cream, Prosweet® Powder, raspberry, root beer, rum, saccharin, safrole, sorbitol, spearmint, spearmint cream, strawberry, strawberry cream, stevia, sucralose, sucrose, sodium saccharin, saccharin, aspartame, acesulfame potassium, mannitol, talin, sucralose, sorbitol, swiss cream, tagatose, tangerine, thaumatin, tutti fruitti, vanilla, walnut, watermelon, wild cherry, wintergreen, xylitol, or any combination of these flavoring ingredients, e.g., anise-menthol, cherry-anise, cinnamon-orange, cherry-cinnamon, chocolate-mint, honey-lemon, lemon-lime, lemon-mint, menthol-eucalyptus, orange-cream, vanilla-mint, and mixtures thereof. In some embodiments, the aqueous liquid dispersion can comprise a sweetening agent or flavoring agent in a concentration ranging from about 0.001% to about 1.0% the volume of the aqueous dispersion. In some embodiments, the aqueous liquid dispersion can comprise a sweetening agent or flavoring agent in a concentration ranging from about 0.005% to about 0.5% the volume of the aqueous dispersion. In yet some embodiments, the aqueous liquid dispersion can comprise a sweetening agent or flavoring agent in a concentration ranging from about 0.01% to about 1.0% the volume of the aqueous dispersion.

[00550] In addition to the additives listed above, the liquid formulations can also include inert diluents commonly used in the art, such as water or other solvents, solubilizing agents, and emulsifiers. Exemplary emulsifiers are ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propyleneglycol, 1,3-butyleneglycol, dimethylformamide, sodium lauryl sulfate, sodium doccusate, cholesterol, cholesterol esters, taurocholic acid, phosphotidylcholine, oils, such as cottonseed oil, groundnut oil, corn germ oil, olive oil, castor oil, and sesame oil, glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols, fatty acid esters of sorbitan, or mixtures of these substances, and the like.

[00551] In some embodiments, the pharmaceutical compositions described herein can be selfemulsifying drug delivery systems (SEDDS). Emulsions are dispersions of one immiscible phase in another, usually in the form of droplets. Generally, emulsions are created by vigorous mechanical dispersion. SEDDS, as opposed to emulsions or microemulsions, spontaneously form emulsions when added to an excess of water without any external mechanical dispersion or agitation. An advantage of SEDDS is that only gentle mixing is required to distribute the droplets throughout the solution. Additionally, water or the aqueous phase can be added just prior to administration, which ensures stability of an unstable or hydrophobic active ingredient. Thus, the SEDDS provides an effective delivery system for oral and parenteral delivery of hydrophobic active ingredients. SEDDS may provide improvements in the bioavailability of hydrophobic active ingredients. Methods of producing self-emulsifying dosage forms are known in the art and include, but are not limited to, for example, U.S. Pat. Nos. 5,858,401, 6,667,048, and 6,960,563, each of which is specifically incorporated by reference.

[00552] It is to be appreciated that there is overlap between the above-listed additives used in the aqueous dispersions or suspensions described herein, since a given additive is often classified differently by different practitioners in the field, or is commonly used for any of several different functions. Thus, the above-listed additives should be taken as merely exemplary, and not limiting, of the types of additives that can be included in formulations described herein. The amounts of such additives can be readily determined by one skilled in the art, according to the particular properties desired.

Intranasal Formulations

[00553] Intranasal formulations are known in the art and are described in, for example, U.S. Pat. Nos. 4,476,116, 5,116,817, and 6,391,452, each of which is specifically incorporated by reference. Formulations that include a compound of any of Formula (L-I), (L-II), and (I) which are prepared according to these and other techniques well-known in the art are prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, fluorocarbons, and/or other solubilizing or dispersing agents known in the art. See, for example, Ansel, H. C. et al., Pharmaceutical Dosage Forms and Drug Delivery Systems, Sixth Ed. (1995). Preferably these compositions and formulations are prepared with suitable nontoxic pharmaceutically acceptable ingredients. These ingredients are known to those skilled in the preparation of nasal dosage forms and some of these can be found in REMINGTON: THE SCIENCE AND PRACTICE OF PHARMACY, 21st edition, 2005, a standard reference in the field. The choice of suitable carriers is highly dependent upon the exact nature of the nasal dosage form desired, e.g., solutions, suspensions, ointments, or gels. Nasal dosage forms generally contain large amounts of water in addition to the active ingredient. Minor amounts of other ingredients such as pH adjusters, emulsifiers or dispersing agents, preservatives, surfactants, gelling agents, or buffering and other stabilizing and solubilizing agents may also be present. The nasal dosage form should be isotonic with nasal secretions. [00554] For administration by inhalation, the compounds of any of Formula (L-I), (L-II), and (I), described herein may be in a form as an aerosol, a mist or a powder. Pharmaceutical compositions described herein are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol, the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of, such as, by way of example only, gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound described herein and a suitable powder base such as lactose or starch.

Buccal Formulations

[00555] Buccal formulations that include compounds of any of Formula (L-I), (L-II), and (I) may be administered using a variety of formulations known in the art. For example, such formulations include, but are not limited to, U.S. Pat. Nos. 4,229,447, 4,596,795, 4,755,386, and 5,739,136, each of which is specifically incorporated by reference. In addition, the buccal dosage forms described herein can further include a bioerodible (hydrolysable) polymeric carrier that also serves to adhere the dosage form to the buccal mucosa. The buccal dosage form is fabricated so as to erode gradually over a predetermined time period, wherein the delivery of the compound of any of Formula (L-I), (L-II), and (I), is provided essentially throughout. Buccal drug delivery, as will be appreciated by those skilled in the art, avoids the disadvantages encountered with oral drug administration, e.g., slow absorption, degradation of the active agent by fluids present in the gastrointestinal tract and/or first-pass inactivation in the liver. With regard to the bioerodible (hydrolysable) polymeric carrier, it will be appreciated that virtually any such carrier can be used, so long as the desired drug release profile is not compromised, and the carrier is compatible with the compound of any of Formula (L- I), (L-II), and (I), and any other components that may be present in the buccal dosage unit. Generally, the polymeric carrier comprises hydrophilic (water-soluble and water-swellable) polymers that adhere to the wet surface of the buccal mucosa. Examples of polymeric carriers useful herein include acrylic acid polymers and co, e.g., those known as “carbomers” (Carbopol®, which may be obtained from B.F. Goodrich, is one such polymer). Other components may also be incorporated into the buccal dosage forms described herein include, but are not limited to, disintegrants, diluents, binders, lubricants, flavoring, colorants, preservatives, and the like. For buccal or sublingual administration, the compositions may take the form of tablets, lozenges, or gels formulated in a conventional manner. Transdermal Formulations

[00556] Transdermal formulations described herein may be administered using a variety of devices which have been described in the art. For example, such devices include, but are not limited to, U.S. Pat. Nos. 3,598,122, 3,598,123, 3,710,795, 3,731,683, 3,742,951, 3,814,097, 3,921,636, 3,972,995, 3,993,072, 3,993,073, 3,996,934, 4,031,894, 4,060,084, 4,069,307, 4,077,407, 4,201,211, 4,230,105, 4,292,299, 4,292,303, 5,336,168, 5,665,378, 5,837,280, 5,869,090, 6,923,983, 6,929,801 and 6,946,144, each of which is specifically incorporated by reference in its entirety.

[00557] The transdermal dosage forms described herein may incorporate certain pharmaceutically acceptable excipients which are conventional in the art. In some embodiments, the transdermal formulations described herein include at least three components: (1) a formulation of a compound of any of Formula (I); (2) a penetration enhancer; and (3) an aqueous adjuvant. In addition, transdermal formulations can include additional components such as, but not limited to, gelling agents, creams and ointment bases, and the like. In some embodiments, the transdermal formulation can further include a woven or non-woven backing material to enhance absorption and prevent the removal of the transdermal formulation from the skin. In some embodiments, the transdermal formulations described herein can maintain a saturated or supersaturated state to promote diffusion into the skin. [00558] Formulations suitable for transdermal administration of compounds described herein may employ transdermal delivery devices and transdermal delivery patches and can be lipophilic emulsions or buffered, aqueous solutions, dissolved and/or dispersed in a polymer or an adhesive. Such patches may be constructed for continuous, pulsatile, or on demand delivery of pharmaceutical agents. Still further, transdermal delivery of the compounds described herein can be accomplished by means of iontophoretic patches and the like. Additionally, transdermal patches can provide controlled delivery of the compounds of any of Formula (L-I), (L-II), and (I). The rate of absorption can be slowed by using rate-controlling membranes or by trapping the compound within a polymer matrix or gel. Conversely, absorption enhancers can be used to increase absorption. An absorption enhancer or carrier can include absorbable pharmaceutically acceptable solvents to assist passage through the skin. For example, transdermal devices are in the form of a bandage comprising a backing member, a reservoir containing the compound optionally with carriers, optionally a rate controlling barrier to deliver the compound to the skin of the host at a controlled and predetermined rate over a prolonged period of time, and means to secure the device to the skin. Injectable Formulations

[00559] Formulations that include a compound of any of Formula (L-I), (L-II), and (I), suitable for intramuscular, subcutaneous, or intravenous injection may include physiologically acceptable sterile aqueous or non-aqueous solutions, dispersions, suspensions or emulsions, and sterile powders for reconstitution into sterile injectable solutions or dispersions. Examples of suitable aqueous and nonaqueous carriers, diluents, solvents, or vehicles including water, ethanol, polyols (propyleneglycol, polyethylene-glycol, glycerol, cremophor and the like), suitable mixtures thereof, vegetable oils (such as olive oil) and injectable organic esters such as ethyl oleate. Proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants. Formulations suitable for subcutaneous injection may also contain additives such as preserving, wetting, emulsifying, and dispensing agents. Prevention of the growth of microorganisms can be ensured by various antibacterial and antifungal agents, such as parabens, chlorobutanol, phenol, sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like. Prolonged absorption of the injectable pharmaceutical form can be brought about by the use of agents delaying absorption, such as aluminum monostearate and gelatin.

[00560] For intravenous injections, compounds described herein may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hank’s solution, Ringer’s solution, or physiological saline buffer. For transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art. For other parenteral injections, appropriate formulations may include aqueous or nonaqueous solutions, preferably with physiologically compatible buffers or excipients. Such excipients are generally known in the art.

[00561] Parenteral injections may involve bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g., in ampoules or in multi-dose containers, with an added preservative. The pharmaceutical composition described herein may be in a form suitable for parenteral injection as a sterile suspensions, solutions, or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Pharmaceutical compositions for parenteral administration include aqueous solutions of the active compounds in water-soluble form. Additionally, suspensions of the active compounds may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Optionally, the suspension may also contain suitable stabilizers or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.

Formulations

[00562] In certain embodiments, delivery systems for pharmaceutical compounds may be employed, such as, for example, liposomes and emulsions. In certain embodiments, compositions provided herein can also include an mucoadhesive polymer, selected from among, for example, carboxymethylcellulose, carbomer (acrylic acid polymer), poly(methylmethacrylate), polyacrylamide, polycarbophil, acrylic acid/butyl acrylate copolymer, sodium alginate and dextran. [00563] In some embodiments, the compounds described herein may be administered topically and can be formulated into a variety of topically administrable compositions, such as solutions, suspensions, lotions, gels, pastes, medicated sticks, balms, creams, or ointments. Such pharmaceutical compounds can contain solubilizers, stabilizers, tonicity enhancing agents, buffers, and preservatives.

[00564] The compounds described herein may also be formulated in rectal compositions such as enemas, rectal gels, rectal foams, rectal aerosols, suppositories, jelly suppositories, or retention enemas, containing conventional suppository bases such as cocoa butter or other glycerides, as well as synthetic polymers such as polyvinylpyrrolidone, PEG, and the like. In suppository forms of the compositions, a low-melting wax such as, but not limited to, a mixture of fatty acid glycerides, optionally in combination with cocoa butter is first melted.

Examples of Methods of Dosing and Treatment Regimens

[00565] The compounds described herein can be used in the preparation of medicaments for the inhibition of menin or a homolog thereof, or for the treatment of diseases or conditions that would benefit, at least in part, from inhibition of menin or a homolog thereof. In addition, a method for treating any of the diseases or conditions described herein in a subject in need of such treatment, involves administration of pharmaceutical compositions containing at least one compound of any of Formula (L-I), (L-II), and (I), described herein, or a pharmaceutically acceptable salt, pharmaceutically acceptable N-oxide, pharmaceutically active metabolite, pharmaceutically acceptable prodrug, or pharmaceutically acceptable solvate thereof, in therapeutically effective amounts to said subject.

[00566] The compositions containing the compound(s) described herein can be administered for prophylactic and/or therapeutic treatments. In therapeutic applications, the compositions are administered to a patient already suffering from a disease or condition, in an amount sufficient to cure or at least partially arrest the symptoms of the disease or condition. Amounts effective for this use will depend on the severity and course of the disease or condition, previous therapy, the patient’s health status, weight, and response to the drugs, and the judgment of the treating physician. It is considered well within the skill of the art for one to determine such therapeutically effective amounts by routine experimentation (including, but not limited to, a dose escalation clinical trial).

[00567] In prophylactic applications, compositions containing the compounds described herein are administered to a patient susceptible to or otherwise at risk of a particular disease, disorder, or condition. Such an amount is defined to be a “prophylactically effective amount or dose.” In this use, the precise amounts also depend on the patient’s state of health, weight, and the like. It is considered well within the skill of the art for one to determine such prophylactically effective amounts by routine experimentation (e.g., a dose escalation clinical trial). When used in a patient, effective amounts for this use will depend on the severity and course of the disease, disorder or condition, previous therapy, the patient’s health status and response to the drugs, and the judgment of the treating physician.

[00568] In the case wherein the patient’s condition does not improve, upon the doctor’s discretion the administration of the compounds may be administered chronically, that is, for an extended period of time, including throughout the duration of the patient’s life in order to ameliorate or otherwise control or limit the symptoms of the patient’s disease or condition.

[00569] In the case wherein the patient’s status does improve, upon the doctor’s discretion the administration of the compounds may be given continuously; alternatively, the dose of drug being administered may be temporarily reduced or temporarily suspended for a certain length of time (i.e., a “drug holiday”). The length of the drug holiday can vary between 2 days and 1 year, including by way of example only, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 10 days, 12 days, 15 days, 20 days, 28 days, 35 days, 50 days, 70 days, 100 days, 120 days, 150 days, 180 days, 200 days, 250 days, 280 days, 300 days, 320 days, 350 days, or 365 days. The dose reduction during a drug holiday may be from 10%-100%, including, by way of example only, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100%. [00570] Once improvement of the patient’s conditions has occurred, a maintenance dose is administered if necessary. Subsequently, the dosage or the frequency of administration, or both, can be reduced, as a function of the symptoms, to a level at which the improved disease, disorder or condition is retained. Patients can, however, require intermittent treatment on a long-term basis upon any recurrence of symptoms.

[00571] The amount of a given agent that will correspond to such an amount will vary depending upon factors such as the particular compound, disease or condition and its severity, the identity (e.g., weight) of the subject or host in need of treatment, but can nevertheless be routinely determined in a manner known in the art according to the particular circumstances surrounding the case, including, e.g., the specific agent being administered, the route of administration, the condition being treated, and the subject or host being treated. In general, however, doses employed for adult human treatment will typically be in the range of 0.02-5000 mg per day, or from about 1-1500 mg per day. The desired dose may conveniently be presented in a single dose or as divided doses administered simultaneously (or over a short period of time) or at appropriate intervals, for example as two, three, four or more sub-doses per day.

[00572] The pharmaceutical composition described herein may be in unit dosage forms suitable for single administration of precise dosages. In unit dosage form, the formulation is divided into unit doses containing appropriate quantities of one or more compound. The unit dosage may be in the form of a package containing discrete quantities of the formulation. Non-limiting examples are packaged tablets or capsules, and powders in vials or ampoules. Aqueous suspension compositions can be packaged in single-dose non-reclosable containers. Alternatively, multiple-dose reclosable containers can be used, in which case it is typical to include a preservative in the composition. By way of example only, formulations for parenteral injection may be presented in unit dosage form, which include, but are not limited to ampoules, or in multi-dose containers, with an added preservative.

[00573] The foregoing ranges are merely suggestive, as the number of variables in regard to an individual treatment regime is large, and considerable excursions from these recommended values are not uncommon. Such dosages may be altered depending on a number of variables, not limited to the activity of the compound used, the disease or condition to be treated, the mode of administration, the requirements of the individual subject, the severity of the disease or condition being treated, and the judgment of the practitioner. [00574] Toxicity and therapeutic efficacy of such therapeutic regimens can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, including, but not limited to, the determination of the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between the toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio between LD50 and ED50. Compounds exhibiting high therapeutic indices are preferred. The data obtained from cell culture assays and animal studies can be used in formulating a range of dosage for use in human. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED50 with minimal toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized.

Combination Treatments

[00575] The Menin-MLL inhibitor compositions described herein can also be used in combination with other well known therapeutic reagents that are selected for their therapeutic value for the condition to be treated. In general, the compositions described herein and, in embodiments where combinational therapy is employed, other agents do not have to be administered in the same pharmaceutical composition, and may, because of different physical and chemical characteristics, have to be administered by different routes. The determination of the mode of administration and the advisability of administration, where possible, in the same pharmaceutical composition, is well within the knowledge of the skilled clinician. The initial administration can be made according to established protocols known in the art, and then, based upon the observed effects, the dosage, modes of administration and times of administration can be modified by the skilled clinician.

[00576] In certain instances, it may be appropriate to administer at least one Menin-MLL inhibitor compound described herein in combination with another therapeutic agent. By way of example only, if one of the side effects experienced by a patient upon receiving one of the Menin-MLL inhibitor compounds described herein is nausea, then it may be appropriate to administer an anti-nausea agent in combination with the initial therapeutic agent. Or, by way of example only, the therapeutic effectiveness of one of the compounds described herein may be enhanced by administration of an adjuvant (i.e., by itself the adjuvant may have minimal therapeutic benefit, but in combination with another therapeutic agent, the overall therapeutic benefit to the patient is enhanced). Or, by way of example only, the benefit experienced by a patient may be increased by administering one of the compounds described herein with another therapeutic agent (which also includes a therapeutic regimen) that also has therapeutic benefit. In any case, regardless of the disease, disorder or condition being treated, the overall benefit experienced by the patient may simply be additive of the two therapeutic agents or the patient may experience a synergistic benefit.

[00577] The particular choice of compounds used will depend upon the diagnosis of the attending physicians and their judgment of the condition of the patient and the appropriate treatment protocol. The compounds may be administered concurrently (e.g., simultaneously, essentially simultaneously or within the same treatment protocol) or sequentially, depending upon the nature of the disease, disorder, or condition, the condition of the patient, and the actual choice of compounds used. The determination of the order of administration, and the number of repetitions of administration of each therapeutic agent during a treatment protocol, is well within the knowledge of the skilled physician after evaluation of the disease being treated and the condition of the patient.

[00578] It is known to those of skill in the art that therapeutically-effective dosages can vary when the drugs are used in treatment combinations. Methods for experimentally determining therapeutically-effective dosages of drugs and other agents for use in combination treatment regimens are described in the literature. For example, the use of metronomic dosing, i.e., providing more frequent, lower doses in order to minimize toxic side effects, has been described extensively in the literature Combination treatment further includes periodic treatments that start and stop at various times to assist with the clinical management of the patient.

[00579] For combination therapies described herein, dosages of the co-administered compounds will of course vary depending on the type of co-drug employed, on the specific drug employed, on the disease or condition being treated and so forth. In addition, when co-administered with one or more biologically active agents, the compound provided herein may be administered either simultaneously with the biologically active agent(s), or sequentially. If administered sequentially, the attending physician will decide on the appropriate sequence of administering protein in combination with the biologically active agent(s).

[00580] In any case, the multiple therapeutic agents (one of which is a compound of Formula (L-I), (L-II), and (I), described herein) may be administered in any order or even simultaneously. If simultaneously, the multiple therapeutic agents may be provided in a single, unified form, or in multiple forms (by way of example only, either as a single pill or as two separate pills). One of the therapeutic agents may be given in multiple doses, or both may be given as multiple doses. If not simultaneous, the timing between the multiple doses may vary from more than zero weeks to less than four weeks. In addition, the combination methods, compositions and formulations are not to be limited to the use of only two agents; the use of multiple therapeutic combinations are also envisioned.

[00581] It is understood that the dosage regimen to treat, prevent, or ameliorate the condition(s) for which relief is sought, can be modified in accordance with a variety of factors. These factors include the disorder from which the subject suffers, as well as the age, weight, sex, diet, and medical condition of the subject. Thus, the dosage regimen actually employed can vary widely and therefore can deviate from the dosage regimens set forth herein.

[00582] The pharmaceutical agents which make up the combination therapy disclosed herein may be a combined dosage form or in separate dosage forms intended for substantially simultaneous administration. The pharmaceutical agents that make up the combination therapy may also be administered sequentially, with either therapeutic compound being administered by a regimen calling for two-step administration. The two-step administration regimen may call for sequential administration of the active agents or spaced-apart administration of the separate active agents. The time period between the multiple administration steps may range from, a few minutes to several hours, depending upon the properties of each pharmaceutical agent, such as potency, solubility, bioavailability, plasma half-life, and kinetic profile of the pharmaceutical agent. Circadian variation of the target molecule concentration may also determine the optimal dose interval.

[00583] In addition, the compounds described herein also may be used in combination with procedures that may provide additional or synergistic benefit to the patient. By way of example only, patients are expected to find therapeutic and/or prophylactic benefit in the methods described herein, wherein pharmaceutical composition of a compound dislcosed herein and /or combinations with other therapeutics are combined with genetic testing to determine whether that individual is a carrier of a mutant gene that is known to be correlated with certain diseases or conditions.

[00584] The compounds described herein and combination therapies can be administered before, during or after the occurrence of a disease or condition, and the timing of administering the composition containing a compound can vary. Thus, for example, the compounds can be used as a prophylactic and can be administered continuously to subjects with a propensity to develop conditions or diseases in order to prevent the occurrence of the disease or condition. The compounds and compositions can be administered to a subject during or as soon as possible after the onset of the symptoms. The administration of the compounds can be initiated within the first 48 hours of the onset of the symptoms, within the first 6 hours of the onset of the symptoms, or within 3 hours of the onset of the symptoms. The initial administration can be via any route practical, such as, for example, an intravenous injection, a bolus injection, infusion over 5 minutes to about 5 hours, a pill, a capsule, transdermal patch, buccal delivery, and the like, or combination thereof. A compound should be administered as soon as is practicable after the onset of a disease or condition is detected or suspected, and for a length of time necessary for the treatment of the disease, such as, for example, from about 1 month to about 3 months. The length of treatment can vary for each subject, and the length can be determined using the known criteria. For example, the compound or a formulation containing the compound can be administered for at least 2 weeks, between about 1 month to about 5 years, or from about 1 month to about 3 years.

Exemplary Therapeutic Agents for Use in Combination with a Menin-MLL inhibitor Compound

[00585] Where the subject is suffering from or at risk of suffering from an autoimmune disease, an inflammatory disease, or an allergy disease, an Menin-MLL inhibitor compound can be used in with one or more of the following therapeutic agents in any combination: immunosuppressants (e.g., tacrolimus, cyclosporin, rapamicin, methotrexate, cyclophosphamide, azathioprine, mercaptopurine, mycophenolate, or FTY720), glucocorticoids (e.g., prednisone, cortisone acetate, prednisolone, methylprednisolone, dexamethasone, betamethasone, triamcinolone, beclometasone, fludrocortisone acetate, deoxycorticosterone acetate, aldosterone), non-steroidal anti-inflammatory drugs (e.g., salicylates, arylalkanoic acids, 2-arylpropionic acids, N-arylanthranilic acids, oxicams, coxibs, or sulphonanilides), Cox-2-specific irreversible inhibitors (e.g., valdecoxib, celecoxib, or rofecoxib), leflunomide, gold thioglucose, gold thiomalate, aurofin, sulfasalazine, hydroxychloroquinine, minocycline, TNF-a binding proteins (e.g., infliximab, etanercept, or adalimumab), abatacept, anakinra, interferon-[3, interferon-y, interleukin-2, allergy vaccines, antihistamines, antileukotrienes, beta-agonists, theophylline, or anticholinergics.

[00586] Where the subject is suffering from or at risk of suffering from a B-cell proliferative disorder (e.g., plasma cell myeloma), the subjected can be treated with a Menin-MLL inhibitor compound in any combination with one or more other anti-cancer agents. In some embodiments, one or more of the anti-cancer agents are proapoptotic agents. Examples of anti-cancer agents include, but are not limited to, any of the following: gossyphol, genasense, polyphenol E, Chlorofusin, all trans-retinoic acid (ATRA), bryostatin, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), 5-aza-2’-deoxycytidine, all trans retinoic acid, doxorubicin, vincristine, etoposide, gemcitabine, imatinib (Gleevec®), geldanamycin, 17-N-Allylamino-17-Demethoxygeldanamycin (17-AAG), flavopiridol, LY294002, bortezomib, trastuzumab, BAY 11-7082, PKC412, or PD184352, Taxol™, also referred to as “paclitaxel”, which is a well-known anti-cancer drug which acts by enhancing and stabilizing microtubule formation, and analogs of Taxol™, such as Taxotere™. Compounds that have the basic taxane skeleton as a common structure feature, have also been shown to have the ability to arrest cells in the G2-M phases due to stabilized microtubules and may be useful for treating cancer in combination with the compounds described herein.

[00587] Other anti-cancer agents that can be employed in combination with an Menin-MLL inhibitor compound include Adriamycin, Dactinomycin, Bleomycin, Vinblastine, Cisplatin, acivicin; aclarubicin; acodazole hydrochloride; acronine; adozelesin; aldesleukin; altretamine; ambomycin; ametantrone acetate; aminoglutethimide; amsacrine; anastrozole; anthramycin; asparaginase; asperlin; azacitidine; azetepa; azotomycin; batimastat; benzodepa; bicalutamide; bisantrene hydrochloride; bisnafide dimesylate; bizelesin; bleomycin sulfate; brequinar sodium; bropirimine; busulfan; cactinomycin; calusterone; caracemide; carbetimer; carboplatin; carmustine; carubicin hydrochloride; carzelesin; cedefingol; chlorambucil; cirolemycin; cladribine; crisnatol mesylate; cyclophosphamide; cytarabine; dacarbazine; daunorubicin hydrochloride; decitabine; dexormaplatin; dezaguanine; dezaguanine mesylate; diaziquone; doxorubicin; doxorubicin hydrochloride; droloxifene; droloxifene citrate; dromostanolone propionate; duazomycin; edatrexate; eflornithine hydrochloride; elsamitrucin; enloplatin; enpromate; epipropidine; epirubicin hydrochloride; erbulozole; esorubicin hydrochloride; estramustine; estramustine phosphate sodium; etanidazole; etoposide; etoposide phosphate; etoprine; fadrozole hydrochloride; fazarabine; fenretinide; floxuridine; fludarabine phosphate; fluorouracil; flurocitabine; fosquidone; fostriecin sodium; gemcitabine; gemcitabine hydrochloride; hydroxyurea; idarubicin hydrochloride; ifosfamide; iimofosine; interleukin II (including recombinant interleukin II, or rlL2), interferon a-2a; interferon a-2b; interferon a-nl; interferon a-n3; interferon 0-la; interferon y-lb; iproplatin; irinotecan hydrochloride; lanreotide acetate; letrozole; leuprolide acetate; liarozole hydrochloride; lometrexol sodium; lomustine; losoxantrone hydrochloride; masoprocol; maytansine; mechlorethamine hydrochloride; megestrol acetate; melengestrol acetate; melphalan; menogaril; mercaptopurine; methotrexate; methotrexate sodium; metoprine; meturedepa; mitindomide; mitocarcin; mitocromin; mitogillin; mitomalcin; mitomycin; mitosper; mitotane; mitoxantrone hydrochloride; mycophenolic acid; nocodazoie; nogalamycin; ormaplatin; oxisuran; pegaspargase; peliomycin; pentamustine; peplomycin sulfate; perfosfamide; pipobroman; piposulfan; piroxantrone hydrochloride; plicamycin; plomestane; porfimer sodium; porfiromycin; prednimustine; procarbazine hydrochloride; puromycin; puromycin hydrochloride; pyrazofurin; riboprine; rogletimide; safingol; safingol hydrochloride; semustine; simtrazene; sparfosate sodium; sparsomycin; spirogermanium hydrochloride; spiromustine; spiroplatin; streptonigrin; streptozocin; sulofenur; talisomycin; tecogalan sodium; tegafur; teloxantrone hydrochloride; temoporfin; teniposide; teroxirone; testolactone; thiamiprine; thioguanine; thiotepa; tiazofurin; tirapazamine; toremifene citrate; trestolone acetate; triciribine phosphate; trimetrexate; trimetrexate glucuronate; triptorelin; tubulozole hydrochloride; uracil mustard; uredepa; vapreotide; verteporfin; vinblastine sulfate; vincristine sulfate; vindesine; vindesine sulfate; vinepidine sulfate; vinglycinate sulfate; vinleurosine sulfate; vinorelbine tartrate; vinrosidine sulfate; vinzolidine sulfate; vorozole; zeniplatin; zinostatin; zorubicin hydrochloride.

[00588] Other anti-cancer agents that can be employed in combination with an Menin-MLL inhibitor compound include: 20-epi-l, 25 dihydroxyvitamin D3; 5-ethynyluracil; abiraterone; aclarubicin; acylfulvene; adecypenol; adozelesin; aldesleukin; ALL-TK antagonists; altretamine; ambamustine; amidox; amifostine; aminolevulinic acid; amrubicin; amsacrine; anagrelide; anastrozole; andrographolide; angiogenesis irreversible inhibitors; antagonist D; antagonist G; antarelix; anti- dorsalizing morphogenetic protein- 1; antiandrogen, prostatic carcinoma; antiestrogen; antineoplaston; antisense oligonucleotides; aphidicolin glycinate; apoptosis gene modulators; apoptosis regulators; apurinic acid; ara-CDP-DL-PTBA; arginine deaminase; asulacrine; atamestane; atrimustine; axinastatin 1; axinastatin 2; axinastatin 3; azasetron; azatoxin; azatyrosine; baccatin III derivatives; balanol; batimastat; BCR/ABL antagonists; benzochlorins; benzoylstaurosporine; beta lactam derivatives; beta-alethine; betaclamycin B; betulinic acid; bFGF inhibitor; bicalutamide; bisantrene; bisaziridinylspermine; bisnafide; bistratene A; bizelesin; breflate; bropirimine; budotitane; buthionine sulfoximine; calcipotriol; calphostin C; camptothecin derivatives; canarypox IL-2; capecitabine; carboxamide-amino-triazole; carboxyamidotriazole; CaRest M3; CARN 700; cartilage derived inhibitor; carzelesin; casein kinase irreversible inhibitors (ICOS); castanospermine; cecropin B; cetrorelix; chlorins; chloroquinoxaline sulfonamide; cicaprost; cis-porphyrin; cladribine; clomifene analogues; clotrimazole; collismycin A; collismycin B; combretastatin A4; combretastatin analogue; conagenin; crambescidin 816; crisnatol; cryptophycin 8; cryptophycin A derivatives; curacin A; cyclopentanthraquinones; cycloplatam; cypemycin; cytarabine ocfosfate; cytolytic factor; cytostatin; dacliximab; decitabine; dehydrodidemnin B; deslorelin; dexamethasone; dexifosfamide; dexrazoxane; dexverapamil; diaziquone; didemnin B; didox; diethylnorspermine; dihydro-5- azacytidine; 9- dioxamycin; diphenyl spiromustine; docosanol; dolasetron; doxifluridine; droloxifene; dronabinol; duocarmycin SA; ebselen; ecomustine; edelfosine; edrecolomab; eflornithine; elemene; emitefur; epirubicin; epristeride; estramustine analogue; estrogen agonists; estrogen antagonists; etanidazole; etoposide phosphate; exemestane; fadrozole; fazarabine; fenretinide; filgrastim; finasteride; flavopiridol; flezelastine; fluasterone; fludarabine; fluorodaunorunicin hydrochloride; forfenimex; formestane; fostriecin; fotemustine; gadolinium texaphyrin; gallium nitrate; galocitabine; ganirelix; gelatinase irreversible inhibitors; gemcitabine; glutathione irreversible inhibitors; hepsulfam; heregulin; hexamethylene bisacetamide; hypericin; ibandronic acid; idarubicin; idoxifene; idramantone; ilmofosine; ilomastat; imidazoacridones; imiquimod; immunostimulant peptides; insulin-like growth factor- 1 receptor inhibitor; interferon agonists; interferons; interleukins; iobenguane; iododoxorubicin; ipomeanol, 4-; iroplact; irsogladine; isobengazole; isohomohalicondrin B; itasetron; jasplakinolide; kahalalide F; lamellarin- N triacetate; lanreotide; leinamycin; lenograstim; lentinan sulfate; leptolstatin; letrozole; leukemia inhibiting factor; leukocyte alpha interferon; leuprolide+estrogen+progesterone; leuprorelin; levamisole; liarozole; linear polyamine analogue; lipophilic disaccharide peptide; lipophilic platinum compounds; lissoclinamide 7; lobaplatin; lombricine; lometrexol; lonidamine; losoxantrone; lovastatin; loxoribine; lurtotecan; lutetium texaphyrin; lysofylline; lytic peptides; maitansine; mannostatin A; marimastat; masoprocol; maspin; matrilysin irreversible inhibitors; matrix metalloproteinase irreversible inhibitors; menogaril; merbarone; meterelin; methioninase; metoclopramide; MIF inhibitor; mifepristone; miltefosine; mirimostim; mismatched double stranded RNA; mitoguazone; mitolactol; mitomycin analogues; mitonafide; mitotoxin fibroblast growth factor-saporin; mitoxantrone; mofarotene; molgramostim; monoclonal antibody, human chorionic gonadotrophin; monophosphoryl lipid A+myobacterium cell wall sk; mopidamol; multiple drug resistance gene inhibitor; multiple tumor suppressor 1 -based therapy; mustard anticancer agent; mycaperoxide B; mycobacterial cell wall extract; myriaporone; N-acetyldinaline; N-substituted benzamides; nafarelin; nagrestip; naloxone+pentazocine; napavin; naphterpin; nartograstim; nedaplatin; nemorubicin; neridronic acid; neutral endopeptidase; nilutamide; nisamycin; nitric oxide modulators; nitroxide antioxidant; nitrullyn; O6-benzylguanine; octreotide; okicenone; oligonucleotides; onapristone; ondansetron; ondansetron; oracin; oral cytokine inducer; ormaplatin; osaterone; oxaliplatin; oxaunomycin; palauamine; palmitoylrhizoxin; pamidronic acid; panaxytriol; panomifene; parabactin; pazelliptine; pegaspargase; peldesine; pentosan polysulfate sodium; pentostatin; pentrozole; perflubron; perfosfamide; perillyl alcohol; phenazinomycin; phenylacetate; phosphatase irreversible inhibitors; picibanil; pilocarpine hydrochloride; pirarubicin; piritrexim; placetin A; placetin B; plasminogen activator inhibitor; platinum complex; platinum compounds; platinum-triamine complex; porfimer sodium; porfiromycin; prednisone; propyl bis-acridone; prostaglandin J2; proteasome irreversible inhibitors; protein A-based immune modulator; protein kinase C inhibitor; protein kinase C irreversible inhibitors, microalgal; protein tyrosine phosphatase irreversible inhibitors; purine nucleoside phosphorylase irreversible inhibitors; purpurins; pyrazoloacridine; pyridoxylated hemoglobin polyoxyethylene conjugate; raf antagonists; raltitrexed; ramosetron; ras famesyl protein transferase irreversible inhibitors; ras irreversible inhibitors; ras- GAP inhibitor; retelliptine demethylated; rhenium Re 186 etidronate; rhizoxin; ribozymes; RII retinamide; rogletimide; rohitukine; romurtide; roquinimex; rubiginone B 1 ; ruboxyl; safingol; saintopin; SarCNU; sarcophytol A; sargramostim; Sdi 1 mimetics; semustine; senescence derived inhibitor 1; sense oligonucleotides; signal transduction irreversible inhibitors; signal transduction modulators; single chain antigen-binding protein; sizofiran; sobuzoxane; sodium borocaptate; sodium phenyl acetate; solverol; somatomedin binding protein; sonermin; sparfosic acid; spicamycin D; spiromustine; splenopentin; spongistatin 1; squalamine; stem cell inhibitor; stem-cell division irreversible inhibitors; stipiamide; stromelysin irreversible inhibitors; sulfinosine; superactive vasoactive intestinal peptide antagonist; suradista; suramin; swainsonine; synthetic glycosaminoglycans; tallimustine; tamoxifen methiodide; tauromustine; tazarotene; tecogalan sodium; tegafur; tellurapyrylium; telomerase irreversible inhibitors; temoporfin; temozolomide; teniposide; tetrachlorodecaoxide; tetrazomine; thaliblastine; thiocoraline; thrombopoietin; thrombopoietin mimetic; thymalfasin; thymopoietin receptor agonist; thymotrinan; thyroid stimulating hormone; tin ethyl etiopurpurin; tirapazamine; titanocene bichloride; topsentin; toremifene; totipotent stem cell factor; translation irreversible inhibitors; tretinoin; triacetyluridine; triciribine; trimetrexate; triptorelin; tropisetron; turosteride; tyrosine kinase irreversible inhibitors; tyrphostins; UBC irreversible inhibitors; ubenimex; urogenital sinus-derived growth inhibitory factor; urokinase receptor antagonists; vapreotide; variolin B; vector system, erythrocyte gene therapy; velaresol; veramine; verdins; verteporfin; vinorelbine; vinxaltine; vitaxin; vorozole; zanoterone; zeniplatin; zilascorb; and zinostatin stimalamer.

[00589] Yet other anticancer agents that can be employed in combination with an Menin-MLL inhibitor compound include alkylating agents, antimetabolites, natural products, or hormones, e.g., nitrogen mustards (e.g., mechloroethamine, cyclophosphamide, chlorambucil, etc.), alkyl sulfonates (e.g., busulfan), nitrosoureas (e.g., carmustine, lomusitne, etc.), or triazenes (decarbazine, etc.). Examples of antimetabolites include but are not limited to folic acid analog (e.g., methotrexate), or pyrimidine analogs (e.g., Cytarabine), purine analogs (e.g., mercaptopurine, thioguanine, pentostatin).

[00590] Examples of natural products useful in combination with an Menin-MLL inhibitor compound include but are not limited to vinca alkaloids (e.g., vinblastin, vincristine), epipodophyllotoxins (e.g., etoposide), antibiotics (e.g., daunorubicin, doxorubicin, bleomycin), enzymes (e.g., L-asparaginase), or biological response modifiers (e.g., interferon alpha).

[00591] Examples of alkylating agents that can be employed in combination an Menin-MLL inhibitor compound include, but are not limited to, nitrogen mustards (e.g., mechloroethamine, cyclophosphamide, chlorambucil, meiphalan, etc.), ethylenimine and methylmelamines (e.g., hexamethlymelamine, thiotepa), alkyl sulfonates (e.g., busulfan), nitrosoureas (e.g., carmustine, lomusitne, semustine, streptozocin, etc.), or triazenes (decarbazine, etc.). Examples of antimetabolites include, but are not limited to folic acid analog (e.g., methotrexate), or pyrimidine analogs (e.g., fluorouracil, floxouridine, Cytarabine), purine analogs (e.g., mercaptopurine, thioguanine, pentostatin.

[00592] Examples of hormones and antagonists useful in combination with an Menin-MLL inhibitor compound include, but are not limited to, adrenocorticosteroids (e.g., prednisone), progestins (e.g., hydroxyprogesterone caproate, megestrol acetate, medroxyprogesterone acetate), estrogens (e.g., diethlystilbestrol, ethinyl estradiol), antiestrogen (e.g., tamoxifen), androgens (e.g., testosterone propionate, fluoxymesterone), antiandrogen (e.g., flutamide), gonadotropin releasing hormone analog (e.g., leuprolide). Other agents that can be used in the methods and compositions described herein for the treatment or prevention of cancer include platinum coordination complexes (e.g., cisplatin, carboblatin), anthracenedione (e.g., mitoxantrone), substituted urea (e.g., hydroxyurea), methyl hydrazine derivative (e.g., procarbazine), adrenocortical suppressant (e.g., mitotane, aminoglutethimide).

[00593] Examples of anti-cancer agents which act by arresting cells in the G2-M phases due to stabilized microtubules and which can be used in combination with an Menin-MLL inhibitor compound include without limitation the following marketed drugs and drugs in development: Erbulozole (also known as R-55104), Dolastatin 10 (also known as DLS-10 and NSC-376128), Mivobulin isethionate (also known as CI-980), Vincristine, NSC-639829, Discodermolide (also known as NVP-XX-A-296), ABT-751 (Abbott, also known as E-7010), Altorhyrtins (such as Altorhyrtin A and Altorhyrtin C), Spongistatins (such as Spongistatin 1, Spongistatin 2, Spongistatin 3, Spongistatin 4, Spongistatin 5, Spongistatin 6, Spongistatin 7, Spongistatin 8, and Spongistatin 9), Cemadotin hydrochloride (also known as LU-103793 and NSC-D-669356), Epothilones (such as Epothilone A, Epothilone B, Epothilone C (also known as desoxyepothilone A or dEpoA), Epothilone D (also referred to as KOS-862, dEpoB, and desoxyepothilone B ), Epothilone E, Epothilone F, Epothilone B N-oxide, Epothilone A N-oxide, 16-aza-epothilone B, 21- aminoepothilone B (also known as BMS-310705), 21 -hydroxy epothilone D (also known as Desoxyepothilone F and dEpoF), 26-fluoroepothilone), Auristatin PE (also known as NSC-654663), Soblidotin (also known as TZT-1027), LS-4559-P (Pharmacia, also known as LS-4577), LS-4578 (Pharmacia, also known as LS-477-P), LS-4477 (Pharmacia), LS-4559 (Pharmacia), RPR-112378 (Aventis), Vincristine sulfate, DZ-3358 (Daiichi), FR-182877 (Fujisawa, also known as WS-9885B), GS-164 (Takeda), GS-198 (Takeda), KAR-2 (Hungarian Academy of Sciences), BSF-223651 (BASF, also known as ILX-651 and LU-223651), SAH-49960 (Lilly/Novartis), SDZ-268970 (Lilly/Novartis), AM-97 (Armad/Kyowa Hakko), AM- 132 (Armad), AM-138 (Armad/Kyowa Hakko), IDN-5005 (Indena), Cryptophycin 52 (also known as LY-355703), AC-7739 (Ajinomoto, also known as AVE-8063A and CS-39.HCI), AC-7700 (Ajinomoto, also known as AVE-8062, AVE-8062A, CS-39-L-Ser.HCI, and RPR-258062A), Vitilevuamide, Tubulysin A, Canadensol, Centaureidin (also known as NSC-106969), T-138067 (Tularik, also known as T-67, TL-138067 and TI-138067), COBRA-1 (Parker Hughes Institute, also known as DDE-261 and WHI-261), H10 (Kansas State University), Hl 6 (Kansas State University), Oncocidin Al (also known as BTO-956 and DIME), DDE-313 (Parker Hughes Institute), Fijianolide B, Laulimalide, SPA-2 (Parker Hughes Institute), SPA-1 (Parker Hughes Institute, also known as SPIKET-P), 3-IAABU (Cytoskeleton/Mt. Sinai School of Medicine, also known as MF-569), Narcosine (also known as NSC-5366), Nascapine, D-24851 (Asta Medica), A-105972 (Abbott), Hemiasterlin, 3-BAABU (Cytoskeleton/Mt. Sinai School of Medicine, also known as MF-191), TMPN (Arizona State University), Vanadocene acetyl acetonate, T-138026 (Tularik), Monsatrol, Inanocine (also known as NSC-698666), 3-1AABE (Cytoskeleton/Mt. Sinai School of Medicine), A-204197 (Abbott), T-607 (Tuiarik, also known as T-900607), RPR- 115781 (Aventis), Eleutherobins (such as Desmethyleleutherobin, Desaetyleleutherobin, Isoeleutherobin A, and Z-Eleutherobin), Caribaeoside, Caribaeolin, Halichondrin B, D-64131 (Asta Medica), D-68144 (Asta Medica), Diazonamide A, A-293620 (Abbott), NPI-2350 (Nereus), Taccalonolide A, TUB-245 (Aventis), A- 259754 (Abbott), Diozostatin, (-)-Phenylahistin (also known as NSCL-96F037), D-68838 (Asta Medica), D-68836 (Asta Medica), Myoseverin B, D-43411 (Zentaris, also known as D-81862), A- 289099 (Abbott), A-318315 (Abbott), HTI-286 (also known as SPA-110, trifluoroacetate salt) (Wyeth), D-82317 (Zentaris), D-82318 (Zentaris), SC-12983 (NCI), Resverastatin phosphate sodium, BPR-OY-007 (National Health Research Institutes), and SSR-250411 (Sanofi).

[00594] Where the subject is suffering from or at risk of suffering from a thromboembolic disorder (e.g., stroke), the subject can be treated with an Menin-MLL inhibitor compound in any combination with one or more other anti-thromboembolic agents. Examples of anti-thromboembolic agents include, but are not limited any of the following: thrombolytic agents (e.g., alteplase anistreplase, streptokinase, urokinase, or tissue plasminogen activator), heparin, tinzaparin, warfarin, dabigatran (e.g., dabigatran etexilate), factor Xa irreversible inhibitors (e.g., fondaparinux, draparinux, rivaroxaban, DX-9065a, otamixaban, LY517717, or YM150), ticlopidine, clopidogrel, CS-747 (prasugrel, LY640315), ximelagatran, or BIBR 1048.

Kits/ Articles of Manufacture

[00595] For use in the therapeutic applications described herein, kits and articles of manufacture are also described herein. Such kits can include a carrier, package, or container that is compartmentalized to receive one or more containers such as vials, tubes, and the like, each of the container(s) including one of the separate elements to be used in a method described herein. Suitable containers include, for example, bottles, vials, syringes, and test tubes. The containers can be formed from a variety of materials such as glass or plastic.

[00596] The articles of manufacture provided herein contain packaging materials. Packaging materials for use in packaging pharmaceutical products are well known to those of skill in the art. See, e.g., U.S. Patent Nos. 5,323,907, 5,052,558 and 5,033,252. Examples of pharmaceutical packaging materials include, but are not limited to, blister packs, bottles, tubes, inhalers, pumps, bags, vials, containers, syringes, bottles, and any packaging material suitable for a selected formulation and intended mode of administration and treatment. A wide array of formulations of the compounds and compositions provided herein are contemplated as are a variety of treatments for any disease, disorder, or condition that would benefit by inhibition of menin, or in which menin is a mediator or contributor to the symptoms or cause.

[00597] For example, the contained s) can include one or more compounds described herein, optionally in a composition or in combination with another agent as disclosed herein. The container(s) optionally have a sterile access port (for example the container can be an intravenous solution bag or a vial having a stopper pierceable by a hypodermic injection needle). Such kits optionally comprising a compound with an identifying description or label or instructions relating to its use in the methods described herein. [00598] A kit will typically may include one or more additional containers, each with one or more of various materials (such as reagents, optionally in concentrated form, and/or devices) desirable from a commercial and user standpoint for use of a compound described herein. Non-limiting examples of such materials include, but not limited to, buffers, diluents, filters, needles, syringes; carrier, package, container, vial and/or tube labels listing contents and/or instructions for use, and package inserts with instructions for use. A set of instructions will also typically be included.

[00599] A label can be on or associated with the container. A label can be on a container when letters, numbers or other characters forming the label are attached, molded or etched into the container itself; a label can be associated with a container when it is present within a receptacle or carrier that also holds the container, e.g., as a package insert. A label can be used to indicate that the contents are to be used for a specific therapeutic application. The label can also indicate directions for use of the contents, such as in the methods described herein.

[00600] In certain embodiments, the pharmaceutical compositions can be presented in a pack or dispenser device which can contain one or more unit dosage forms containing a compound provided herein. The pack can for example contain metal or plastic foil, such as a blister pack. The pack or dispenser device can be accompanied by instructions for administration. The pack or dispenser can also be accompanied with a notice associated with the container in form prescribed by a governmental agency regulating the manufacture, use, or sale of pharmaceuticals, which notice is reflective of approval by the agency of the form of the drug for human or veterinary administration. Such notice, for example, can be the labeling approved by the U.S. Food and Drug Administration for prescription drugs, or the approved product insert. Compositions containing a compound provided herein formulated in a compatible pharmaceutical carrier can also be prepared, placed in an appropriate container, and labeled for treatment of an indicated condition.

EXAMPLES

[00601] The following specific and non-limiting examples are to be construed as merely illustrative, and do not limit the present disclosure in any way whatsoever. Without further elaboration, it is believed that one skilled in the art can, based on the description herein, utilize the present disclosure to its fullest extent. All publications cited herein are hereby incorporated by reference in their entirety. Where reference is made to a URL or other such identifier or address, it is understood that such identifiers can change and particular information on the internet can come and go, but equivalent information can be found by searching the internet. Reference thereto evidences the availability and public dissemination of such information.

[00602] The examples below as well as throughout the application, the following abbreviations have the following meanings. If not defined, the terms have their generally accepted meanings. aq = aqueous

Boc = tert-butyl oxy carbonyl Z-BuOH = tertiary butanol

DCE = 1,2-di chloroethane DCM = dichloromethane

DIAD = diisopropyl azodi carboxyl ate

DIEA or DIPEA = N,N-diisopropylethylamine

DMAP = dimethylaminopyridine DMF = dimethylformamide

DMSO = dimethylsulfoxide ESI = electron spray ionization EA = ethyl acetate g = gram

HC1 = hydrogen chloride

HPLC = high performance liquid chromatography hr = hour = proton nuclear magnetic resonance IPA = isopropyl alcohol

KOAc = potassium acetate

LC-MS = liquid chromatography mass spectroscopy M = molar MeCN = acetonitrile MeOH = methanol mg = milligram min = minute ml = milliliter mM = millimolar mmol = millimole m.p. = melting point

MS = mass spectrometry mlz = mass-to-charge ratio

N = normal

NIS = N-iodosuccinimide nM = nanomolar nm = nanometer

Pd(dppf)C12 = [1 ,r-Bis(diphenylphosphino)ferrocene]dichloropalladium(II)

PE = petroleum ether

PyBOP = benzotriazol- 1-yl-oxytripyrrolidinophosphonium hexafluorophosphate quant. = quantitative

RP = reverse phase rt or r.t. = room temperature

Sat. = saturated

TEA = triethylamine

TFA = trifluoroacetic acid p.L = microliter pM = Micromolar

[00603] A solution of morpholine (3.12 g, 35.7 mmol, 3.15 mL, 2 eq) and Intermediate 1A (5.00 g, 17.8 mmol, 1 eq) in n-butanol (25.0 mL) was heated at 100°C for 12 h. The color of the solution became white. TLC (Dichloromethane/Methanol = 10/1, Rf = 0.60) indicated the starting material was consumed completely. The reaction mixture was diluted with H2O (200.0 mL) and extracted with EtOAc (100.0 mL x 3). The combined organic layers were washed with brine (100.0 mL), dried over NazSCh, filtered, and concentrated under reduced pressure to give a residue. The compound was used for the next step without further purification. Intermediate 3A (5.08 g, 15.3 mmol, 86.0% yield) was obtained as a white solid

General procedure for preparation of Intermediate 5A

[00604] A solution of (4-aminophenyl)boronic acid (2.49 g, 18.1 mmol, 1.5 eq), Intermediate 3 A (4.00 g, 12.1 mmol, 1 eq) and K2CO3 (10.0 g, 72.7 mmol, 6 eq) in dioxane (20.0 mL) and H2O (4.00 mL) was degassed with argon 30 min. Cyclopentyl(diphenyl)phosphane; dichloropalladium;iron (886.5 mg, 1.21 mmol, 0.1 eq) was added to the reactor. The mixture was refluxed at 100°C for 12 h. The color of the solution become black. TLC (Dichloromethane/Methanol = 10/1, Rf = 0.57) indicated the starting material was consumed completely. The reaction mixture was diluted with H2O (300.0 mL) and extracted with EtOAc (300.0 mL x 3). The combined organic layers were washed with brine (300.0 mL), dried over Na2SO4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO2, Petroleum ether/Ethyl acetate = 50/1 to 5/1) Intermediate 5 A (3.00 g, 10.1 mmol, 83.8% yield) was obtained as a white solid. Synthesis of Intermediate - D

[00605] To a stirred solution of Intermediate 1 (3.00 g, 17.9 mmol, 1 eq) in CHCh (20.0 mL) were added TEA (2.74 g, 27.1 mmol, 3.77 mL, 1.51 eq) and methanesulfonyl chloride (2.32 g, 20.2 mmol, 1.57 mL, 1.13 eq) at 0°C. The mixture was stirred at 0°C for 2 h. TLC (Dichloromethane : Methanol = 10 : 1, Rf = 0.62) showed the reaction was complete. The mixture was poured into ice / H2O (40.0 mL) and extracted with DCM (30.0 mL x 3). Then the organic phases were washed with brine (50.0 mL) then dried over Na2SO4, filtered and concentrated under vacuum. The crude was used in the next step without purification. Intermediate 2 (3.63 g, crude) was obtained as a yellow solid.

'H NMR : CDCL _400MHz 8.80 (d, J= 4.85 Hz, 1H), 8.15 (d, J= 0.66 Hz, 1H), 7.53 (dt, J= 4.91, 0.85 Hz, 1H), 5.27 - 5.34 (m, 2H), 4.00 - 4.08 (m, 3H), 3.11 (s, 3H)

General procedure for preparation of Intermediate 4 -

[00606] A mixture of Intermediate 2 (2.50 g, 10.1 mmol, 1 eq) and 3 (4.08 g, 20.3 mmol, 2 eq), K2CO3 (7.04 g, 50.9 mmol, 5 eq) in DMF (25.0 mL) were degassed and purged with N2 for 3 times, and then the mixture was stirred at 120°C for 5 h under N2 atmosphere. TLC (Dichloromethane:Methanol = 10 : 1, Rf = 0.55) showed the reaction was complete. The mixture was poured into H2O (70.0 mL) and extracted with DCM (40.0 mL x 3). Then the organic phases were washed with brine (100.0 mL) dried over Na2SO4, filtered and concentrated under vacuum. The residue was purified by silica gel chromatography eluted with Petroleum ether : Ethyl acetate = 100/1 ~ 20/1 ~ 10/1-1/1. Intermediate 4 (1.85 g, 5.29 mmol, 51.9% yield) was obtained as a yellow solid.

'H NMR: CDCL _400MHz 8.68 (d, J= 5.07 Hz, 1H), 8.07 (s, 1H), 7.46 - 7.49 (m, 1H), 4.90 (br s, 1H), 4.01 (s, 3H), 3.54 (s, 2H), 2.61 (br d, J= 8.82 Hz, 1H), 2.20 - 2.43 (m, 3H), 1.69 (br s, 2H), 1.52 - 1.62 (m, 1H), 1.44 (s, 9H)

General procedure for preparation of Intermediate 5 -

[00607] To a solution of Intermediate 4 (1.50 g, 4.29 mmol, 1 eq) in THF (7.00 mL) was added LiOH.H2O (540.3 mg, 12.8 mmol, 3 eq) in H2O (7.00 mL). The mixture was stirred at 25 °C for 3 h. TLC (Dichloromethane : Methanol = 10 : 1, Rf= 0) showed the reaction was complete. The mixture was poured into H2O (20.0 mL) and extracted with DCM (10.0 mL x 3). Then the organic phases dried over ISfeSCL, filtered and concentrated under vacuum. The crude was used without purification. Intermediate 5 (1.20 g, crude) was obtained as a yellow solid.

'H NMR : DMSO _400MHz 8.47 (br s, 1H), 7.86 (br s, 1H), 7.20 - 7.37 (m, 1H), 6.71 (br d, J= 7.50 Hz, 1H), 3.48 (br d, J= 13.01 Hz, 3H), 2.65 - 2.78 (m, 1H), 1.74 - 1.87 (m, 2H), 1.68 (br d, J= 7.94 Hz, 2H), 1.58 (br d, J= 11.91 Hz, 1H), 1.37 (br d, J= 7.06 Hz, 3H), 1.35 (s, 9H).

General procedure for preparation of Intermediate 6 -

[00608] To a solution of Intermediate 5 (0.80 g, 2.39 mmol, 1 eq), Intermediate 3 A (704.4 mg, 2.39 mmol, 1 eq), and TEA (1.69 g, 16.7 mmol, 2.32 mL, 7 eq) in DCM (10.0 mL) was added HATU (1.36 g, 3.58 mmol, 1.5 eq). The mixture was stirred at 20°C for 12 h. LCMS showed the reaction was complete. The mixture was poured into H2O (40.0 mL) and extracted with DCM (20.0 mL x 3). Then the organic phases were washed with brine (50.0 mL) dried over Na2SO4, filtered and concentrated under vacuum. The crude for next step without purification. Intermediate 6 (0.60 g, crude) was obtained as a yellow solid.

General procedure for preparation of Intermediate 7 -

[00609] To a solution of Intermediate 6 (0.50 g, 816.0 umol, 1 eq) in MeOH (5.00 mL) was added HCI/MeOH (4 M, 5.00 mL, 24.51 eq). The mixture was stirred at 20°C for 12 h. LCMSshowed the reaction was complete. The mixture was concentrated under vacuum. The crude for next step without purification. Intermediate 7 (0.50 g, crude, HC1 salt) was obtained as a yellow solid. General procedure for preparation of Intermediate D -

Procedure 1

[00610] To a solution of Intermediate 7 (0.50 g, 910.6 umol, 1 eq, HC1) in DMF (10.0 mL) was added TEA (645.0 mg, 6.37 mmol, 887.2 uL, 7 eq} and 4-bromobut-2-enoyl chloride (910.6 umol). Then the mixture was stirred at 20°C for 12 h. LCMS showed the reaction is complete. The mixture was poured into H2O (50.0 mL), then was filtered and filter cake was concentrated in vacuum. The crude product was purified by reversed-phase HPLC (column : Phenomenex Luna C18 200*40mm*10um; mobile phase : (water(0.05%HCl)-ACN); B%: 10%-30%, lOmin) and (column : Xtimate C18 150*25mm*5um; mobile phase : [water(10mM NH4HCO3)-ACN]; B%: 30%-60%, lOmin). Intermediate D was obtained as a brown solid.

Procedure 2

[00611] To a solution of compound 7 (4.60 g, 9.00 mmol) and DIEA (9.30 g, 71.9 mmol) in DMF (46.0 mL) was added 4-bromobut-2-enoyl chloride (3.30 g, 17.9 mmol) at 25 °C. The mixture was stirred at 25 °C for 12 hrs. LCMS showed the starting material was consumed completely. The reaction mixture was quenched by addition H2O (50.0 mL) at 25 °C, filtered and concentrated under reduced pressure to give a residue. The crude product was used for the next step without further purification. Compound D (4.00 g, 6.06 mmol, 67.4% yield) was obtained as a brown solid.

Intermediate 8: Synthesis of 4-(4-chloro-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)aniline (8b):

[00612] To a stirred solution of 4-chloro-6-iodo-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H- pyrrolo[2,3-d]pyrimidine 8a (20.0 g, 48.8 mmol) in 1,4-Dioxane (120 mL) and H2O (24.0 mL), were added (4-aminophenyl)boronic acid hydrochloride (10.20 g, 58.6 mmol) and NazCOs (15.5 g, 146 mmol) at room temperature. The reaction mixture was degassed with N2 for 15 min. Then PdC12(dppf). DCM (3.57 g, 4.88 mmol) was added and heated to 80°C for 16h. After the reaction was complete (TLC monitoring), the resulting reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (3 x 500 mL). The combined organic layer was washed with brine (2 x 200 mL), dried over ISfeSCU , filtered and evaporated under reduced pressure to get the crude residue which was purified by flash column chromatography (silica gel, 120 g SNAP) by using eluent 10% ethyl acetate in heptane to get the desired product as brown solid 8b (8.20 g, 44%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8.63 (s, 1H), 7.51-7.49 (d, J= 8.4 Hz, 2H), 6.69-6.67 (d, J= 8.4 Hz, 2H), 6.63 (s, 1H), 5.59-5.58 (m, 4H), 3.66-3.62 (q, J= 7.6 Hz, 15.5Hz, 2H), 0.88-0.84 (t, J= 8.0 Hz, 2H) and 0.08 (s, 9H). LCMS= [M+H] ⁺ : (375.22), Purity= 96%

Synthesis of 4-(4-methyl-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)aniline (8c):

[00613] To a stirred solution of 4-(4-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[ 2,3- d]pyrimidin-6-yl)aniline 8b (6.00 g, 16.0 mmol) in THF (80.0 mL) was added Palladium- tetrakis(triphenylphosphine) (1.85 g, 1.60 mmol). The resultant reaction mixture was degassed with N2 for 15 min and trimethyl aluminum (2.0 M in Toluene; 16.0 mL, 32.0 mmol) was added in drop wise manner. The resulting reaction mixture was heated to 80°C for 2h. After completion of reaction (TLC monitoring), the reaction mixture was cooled and poured into ice cold aqueous NH4CI solution followed by extraction with CHCk (3 x 250 mL). The combined organic layer was washed with brine solution (2 x 200 mL), dried over Na2SO4 , filtered and evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40g SNAP) using eluent 30% ethyl acetate in heptane to get the desired product as viscous liquid 8c (3.60 g, 63%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8.80 (s, 1H), 8.63 (s, 1H), 7.46-7.44 (d, J= 8.4 Hz, 2H), 6.68-6.66 (m, 3H), 5.54-5.46 (m, 3H), 3.64-3.58 (q, J= 7.6 Hz, 15.5Hz, 2H), 2.65 (s, 3H), 0.88-0.84 (t, J= 8.0 Hz, 2H) and 0.08 (s, 9H). LCMS= [M+H] ⁺ : (355.23), Purity= 87% Synthesis of tert-butyl (R)-(l-((2-((4-(4-methyl-7-((2-(trimethylsilyl)ethoxy)methyl )-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)m ethyl)piperidin-3-yl)carbamate (8d):

[00614] To a stirred solution of 4-(4-methyl-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[ 2,3- d]pyrimidin-6-yl)aniline 8c (250 mg, 705 mmol) in DMF (10 mL), were added and 4-{[(3R)-3- {[(tert-butoxy)carbonyl]amino}piperidin-l-yl]methyl}pyridine -2-carboxylic acid, intermediate 6 (284 mg, 846 mmol), DIPEA (0.626 mL, 3.53 mmol) and HATU (322 mg, 846 mmol) at room temperature. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (20 mL) and extracted with ethyl acetate (3 x 150 mL). The combined organics was washed brine solution (3 x 100 mL), dried over ISfeSCU, filtered and evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) using eluent 50% ethyl acetate in heptane to get the desired product as viscous liquid 8d (300 mg, 63%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 10.87 (s, 1H), 8.80 (s, 1H), 8.12-8.09 (m, 2H), 7.79-7.72 (m, 2H), 7.63-7.61 (m, 1H), 6.93 (s, 1H), 6.76-6.66 (m, 1H), 5.67 (s, 2H), 3.66-3.60 (m, 4H), 3.50-3.48 (m, 1H), 2.82-2.80 (m, 1H), 2.75-2.65 (m, 4H), 2.08-1.50 (m, 6H), 1.49 (s, 7H), 1.60-1.26 (br s, 4H), 0.88- 0.84 (m, 2H) and -0.09 (s, 9H). LCMS= [M+H] ⁺ : (672.25), Purity= 82%

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-methyl-7H-pyrr olo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide (intermediate 8):

[00615] To an ice cold solution of tert-butyl N-[(3R)-l-[(2-{[4-(4-methyl-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl]carbamoyl}pyri din-4- yl)methyl]piperidin-3-yl]carbamate 8d (300 mg, 446 mmol) in DCM (2.00 mL), was added TFA (5.00 mL) in drop wise manner. The resulting reaction mixture was allowed to stir at room temperature for 4 days. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue which was triturated with di-ethyl ether (50 mL). The sticky solids so obtained was treated with aqueous NH4OH solution, solid precipitated out, filtered off and washed with water (2 x 25 mL) to get the desired product as yellow solid intermediate 8 (180 mg, 91%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 10.80 (s, 1H), 8.69-8.68 (m, 1H), 8.59-8.58 (m, 1H), 8.12-7.91 (m, 5H), 7.61-7.60 (m, 1H), 7.08-7.07 (m, 1H), 3.64-3.60 (m, 2H), 2.68-2.66 (m, 6H), 2.08-1.77 (m, 5H), 1.55-1.50 (m, 2H) and 1.16-1.04 (m, 2H). LCMS= [M+H]+: (442.25), Purity = 94%. REAGENTS

Synthesis of RG1:

Synthesis of tert-butyl (E)-4-bromobut-2-enoate (RGlb):

[00616] To a stirred solution of tert-butyl (E)-but-2-enoate RGla (5.00 g, 35.2 mmol) in CCI4 was added NBS (6.26 g, 35.2 mmol) and Benzoyl peroxide (256.0 mg, 1.05 mmol) at room temperature under N2 atmosphere. The reaction mass was refluxed 80°C for 12h. After completion of reaction (TLC monitoring), the resulting reaction mixture was cooled to room temperature, filtered through celite bed followed by washing with DCM (2 x 20 mL). The combined organic layer was evaporated under reduced pressure to get the crude residue which was purified over silica gel (100-200M) column chromatography using eluents 10% ethyl acetate in heptane to get the desired product as yellow liquid RGlb (5.00 g, 64%).

'H NMR (400 MHz, CDCh): 6.93-6.85 (m, 1H), 5.98-5.96 (d, J= 15.2 Hz, 1H), 3.99-3.98 (d, J= 7.2 Hz, 2H) and 1.48 (s, 9H). MS= [M+H]+: (221.27)

Synthesis of tert-butyl-4-(3-fluoroazetidin-l-yl)but-2-enoate (RGlc):

[00617] To a stirred solution of tert-butyl (E)-4-bromobut-2-enoate RGlb (1.00 g, 4.52 mmol) and 3 -fluoroazetidine hydrochloride 14 (505 mg, 4.52 mmol) in THF (30. 0 mL) was added triethylamine (1.61 mL, 9.04 mmol) at 0°C under N2 atmosphere. The reaction mixture was stirred at room temperature for 16h. After completion of reaction progress (TLC monitoring), the resulting solid suspension was filtered followed by washing with THF (2 x 5 mL). The filtrate was evaporated under reduced pressure to get the crude residue which was purified over silica gel (100-200M) column chromatography using eluents 10% ethyl acetate in heptane to get desired product as colorless sticky compound RGlc (700 mg, 72%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 6.67-6.60 (m, 1H), 3.60-3.52 (m, 2H), 3.32-3.30 (d, J= 9.6 Hz, 2H), 3.23-3.21 (d, J= 4.8 Hz, 2H), 3.16-3.06 (m, 2H) and 1.42 (s, 9H). MS= [M+H]+: (216.27) Synthesis of (E)-4-(3-fluoroazetidin-l-yl)but-2-enoic acid (RG1):

[00618] To an ice cold solution of tert-butyl (E)-4-(3-fluoroazetidin-l-yl)but-2-enoate RGlc (700 mg, 3.25 mmol) in DCM (10 mL) was added 4N-HC1 (7.00 mL). The resultant reaction mixture was stirred at room temperature for 16h. After completion of reaction progress (TLC monitoring), the reaction mixture was concentrated under reduced pressure and azeotrope with toluene (10 mL) to get crude residue as white solid 11 (450 mg, 87%). ¹ HNMR (400 MHz, DMSO-t/ ₆ ): 12.07 (brs, 1H), 6.71-6.49 (m, 1H), 6.24-6.17 (m, 1H), 5.45-5.23 (m, 1H), 4.29-4.20 (m, 4H) and 4.06-4.04 (d, J= 4.8 Hz, 2H). MS= [M+H]+: (160.11)

Reagent RG2

Synthesis of tert-butyl (E)-4-(3,3-difluoroazetidin-l-yl)but-2-enoate (RG2a):

[00619] To an ice cold solution of tert-butyl (E)-4-bromobut-2-enoate RGlb (1.0 g, 4.52 mmol) and 3,3-difluoroazetidine hydrochloride (702 mg, 5.42 mmol) in THF (30 mL) was added triethylamine (1.57 mL, 11.3 mmol) at room temperature. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting solid suspension was filtered off followed by washing with THF (2 x 10 mL). The combined organic was evaporated under reduced pressure to get the crude oil which was purified by flash chromatography (silica gel, 40 g SNAP) using eluent 20% ethyl acetate in heptane to get the desired product as light brown oil RG2a (450.0 mg, 42%).

'H NMR (400 MHz, CDCh): 6.76-6.70 (m, 1H), 5.90-5.86 (d, J= 15.6Hz, 1H), 3.66-3.38 (m, 4H), 3.32-3.30 (m, 2H) and 1.47 (s, 9H).

MS= [M+H]+: (234.11).

Synthesis of (E)-4-(3,3-difluoroazetidin-l-yl)but-2-enoic acid (RG2)

[00620] To an ice cold stirred solution of tert-butyl (E)-4-(3,3-difluoroazetidin-l-yl)but-2-enoate RG2a (450 mg, 1.93 mmol) in DCM (10 mL), was added 4N-HC1 (6.0 mL). The resultant reaction mixture was stirred at room temperature for 16h. After completion of reaction s (TLC monitoring), solvent was evaporated under reduced pressure and thus obtained crude residue was co-evaporated with toluene (10 mL) to get desired product as white solid RG2 (341 mg, quantitative).

^X H NMR (400 MHz, MeOD- d ₄ y. 6.81-6.76 (m, 1H), 6.27-6.23 (m, 1H), 4.84-4.81 (m, 2H) and 4.22- 4.20 (d, ./=6,4 Hz, 4H). LCMS= [M+H]+: (178.27), Purity= 95%. Reagent RG3

Synthesis of tert-butyl (E)-4-(azetidin-l-yl)but-2-enoate (RG3a):

[00621] To an ice cold solution of tert-butyl (2E)-4-bromobut-2-enoate RGlb (2.60 g, 11.75 mmol) and azetidine hydrochloride (1.00 g, 0.69 mmol) in THF (50 mL) was added triethylamine (3.72 mL, 26.7 mmol) under N2 atmosphere. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting solid suspension was filtered off followed by washing with THF (2 x 10 mL). The combined organic layer was dried over ISfeSCU, filtered and evaporated under reduced pressure to get the crude oil which was purified (silica gel, 40 g SNAP) using eluent 40% ethyl acetate in heptane to get the desired light brown oil RG3a (560 mg, 26%).

'H NMR (400 MHz, DMSO-t/ ₆ : 7.81-7.79 (m, 1H), 6.76-6.74 (m, 1H), 3.64-3.60 (m, 4H), 2.68- 2.62(m, 2H), 2.32-2.30 (m, 2H) and 1.42 (s, 9H) MS= [M+H] ⁺ : (198.14), Purity= 90%

Synthesis of (E)-4-(azetidin-l-yl)but-2-enoic acid (RG3):

[00622] To an ice cold solution of tert-butyl (2E)-4-(azetidin-l-yl)but-2-enoate RG3a (500 mg, 2.53 mmol) in DCM (10 mL) was added 4N-HC1 (5.0 mL) and resultant reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure and azeotrope with toluene (10 mL) to get crude residue (2E)- 4-(azetidin-l-yl)but-2-enoic acid RG3 (320 mg, 89%) as white solid.

^X H NMR (400 MHz, MeODWv): 6.81-6.76 (m, 1H), 6.27-6.23 (m, 1H), 4.84-4.82 (m, 4H) and 4.22- 4.20 (m, 4H) MS= [M+H] ⁺ : (142.19), Purity= 90%.

[00623] To a solution of compound D (1.00 g, 1.52 mmol, 1.00 eq) in DMF (5.00 mL) were added K2CO3 (1.05 g, 7.58 mmol, 5.00 eq) and compound E (259 mg, 2.77 mmol, 306 uL, 1.83 eq, HC1). The mixture was stirred at 25 °C for 12 hrs. LCMS showed compound D was consumed completely and one main peak with desired m/z was detected. The reaction mixture was quenched by addition H2O (60.0 mL) at 20°C, filtered and was concentrated under reduced pressure to give a residue. The mixture was further purified by pre-HPLC to yield Compound 101 column: Phenomenex luna Cl 8 80*40mm*3 um; mobile phase: [water (0.04%HCl)-ACN]; B%: 13%-20%,3.5 min. Compound 1 (140 mg, 220 umol, 14.5% yield) was obtained as an orange solid.

'H NMR: (400 MHz, D ₂ O) 8 8.84 (d, J = 5.0 Hz, 1H), 8.33 - 8.18 (m, 2H), 7.84 - 7.66 (m, 5H), 7.12 - 7.06 (m, 1H), 6.77 - 6.56 (m, 1H), 6.44 - 6.26 (m, 1H), 4.61 - 4.52 (m, 2H), 4.34 - 4.06 (m, 4H), 4.04 - 3.93 (m, 10H), 3.74 - 3.46 (m, 2H), 3.31 - 2.96 (m, 2H), 2.93 - 2.84 (m, 2H), 2.67 - 2.43 (m, 1H), 2.22 - 1.49 (m, 4H).

[00624] To a solution of D (2.00 g, 3.03 mmol) in DMF (20.0 mL) was added K2CO3 (1.26 g, 9.10 mmol) and pyrrolidine (646 mg, 9.10 mmol). The mixture was stirred at 25 °C for 12 hrs. LCMS showed the starting material was consumed completely. The reaction mixture was quenched by addition H2O (50.0 mL) at 25°C, filtered and concentrated under reduced pressure to give a residue. The mixture was further purified by pre-HPLC (column: Phenomenex luna C18 80*40mm*3 um; mobile phase: (water (0.04%HCl)-ACN); B% 5%-23%, 7min. Compound 102 (0.1 g, 0.15 mmol, 5.1% yield) was obtained as an orange solid.

'H NMR: (400 MHz, D ₂ O) 8 8.73 (d, J = 4.88 Hz, 1H), 8.17 (s, 1H), 7.95 (s, 1H), 7.69 (d, J= 4.63 Hz, 1H), 7.26 - 7.51 (m, 4H), 6.75 (s, 1H), 6.59 - 6.71 (m, 1H), 6.30 (d, J=15.4 Hz, 1H), 4.48 (s, 2H) 4.15 (br s, 1H), 3.83 - 4.03 (m, 6H), 3.76 (br s, 4H), 3.41 - 3.64 (m, 4H), 2.82 - 3.18 (m, 4H), 1.48 - 2.18 (m, 8H).

[00625] To a solution of D (2.00 g, 3.03 mmol) in DMF (20.0 mL) were added K2CO3 (1.26 g, 9.10 mmol) and piperidine (646 mg, 9.10 mmol). The mixture was stirred at 25 °C for 12 hrs. LCMS showed the starting material was consumed completely. The reaction mixture was quenched by addition H2O (50.0 mL) at 25°C, filtered and concentrated under reduced pressure to give a residue. The mixture was further purified by pre-HPLC (column: Phenomenex luna C18 80*40mm*3 um; mobile phase: [water(0.04%HCl)-ACN]; B% 5%-23%,7min). Compound 103 (0.1 g, 0.15 mmol, 5.1% yield) was obtained as a light orange solid.

'H NMR: (400 MHz, D ₂ O ) <5 8.77 (d, J= 4.88 Hz, 1H), 8.22 (s, 1H), 8.10 (s, 1H), 7.73 (d, J= 4.8 Hz, 1H), 7.50 - 7.67 (m, 4H), 6.97 (s, 1H), 6.64 (dt, J= 15.2, 7.35 Hz, 1H), 6.30 (d, J=15.2 Hz, 1H), 4.51 (s, 2H), 4.16 (s, 1H), 3.77 - 4.00 (m, 11H), 3.42 (s, 3H), 2.66 - 3.05 (m, 3H), 1.52 - 2.13 (m, 10H), 1.38 (d, J=12 Hz, 1H).

Synthesis of (R,E)-4-((3-(4-(3-fluoroazetidin-l-yl)but-2-enamido)piperidi n-l-yl)methyl)-N-(4- (4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolin amide (Example 4):

[00626] To an ice cold stirred solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4- morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinami de 7 (150 mg, 0.29 mmol) in DMF (1.00 mL) were added (E)-4-(3-fluoroazetidin-l-yl)but-2-enoic acid RG1 (56 mg, 0.35 mmol) and DIPEA (0.26 mL, 1.46 mmol). Then T3P (50% in ethyl acetate; 0.65mL, 1.02 mmol) was added drop wise at 0°C. The resulting reaction mixture was stirred at room temperature for next 2h. After completion of reaction (TLC monitoring), the resulting reaction mixture was concentrated to dryness and crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) using 2-3% MeOH in DCM as eluent to get the desired product as white solid 104 (40 mg, 20%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 12.2 (s, 1H), 10.74 (s, 1H), 8.68-8.66 (d, J= 5.2 Hz, 1H), 8.18 (s, 1H), 8.11 (s, 1H), 8.01-7.98 (d, J= 8.8 Hz, 2H), 7.92-7.90 (m, 3H), 7.62-7.61 (d, J= 4.4 Hz, 1H), 7.16 (s, 1H), 6.47- 6.41 (m, 1H), 6.03-5.99 (d,J= 15.6 Hz, 1H), 5.24-5.08 (m, 1H), 3.89-3.88 (m, 4H), 3.76- 3.74 (m, 6H), 3.65 (s, 2H), 3.57 (brs, 2H), 3.20-3.13 (m, 4H), 2.77-2.75 (m, 1H), 2.66-2.58 (m, 1H), 2.06-2.01 (m, 1H), 1.90-1.85 (m, 1H), 1.75-1.67 (m, 2H) and 1.54-1.51 (m, 1H) LCMS= [M+H] ⁺ : (654.27), Purity = 98%.

Compound 105

Synthesis of (R,E)-4-((3-(4-(3,3-difluoroazetidin-l-yl)but-2-enamido)pipe ridin-l-yl)methyl)-N- (4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)pico linamide (Example 5):

[00627] To an ice cold stirred solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4- morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinami de hydrochloride 7 (150 mg, 273 mmol) in DMF (1 mL) were added (E)-4-(3,3-difluoroazetidin-l-yl)but-2-enoic acid RG2 (48 mg, 273 mmol), DIPEA (0.24 mL, 1.36 mmol) and T3P (50% in ethyl acetate ; 520 mL, 819 mmol). The reaction mixture was stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated to dryness and purified by flash column chromatography (silica gel, 12 g SNAP) using 2-3% MeOH in DCM as eluent to get the desired product as white solid 105 (35 mg, 19%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 12.2 (s, 1H), 10.74 (s, 1H), 8.68-8.67 (d, J= 4.0 Hz, 1H), 8.18 (s, 1H), 8.11 (s, 1H), 8.01-7.99 (d, J= 8.4 Hz, 2H), 7.92-7.90 (m, 3H), 7.62-7.61 (d, J= 4.0 Hz, 1H), 7.16 (s, 1H), 6.50- 6.44 (m, 1H), 6.05-6.01 (m, 1H), 3.88-3.87 (m, 4H), 3.75-3.69 (m, 6H), 3.66-3.64 (m, 2H), 3.62-3.55 (m, 4H), 3.32-3.30 (m, 1H), 2.77-2.75 (m, 1H), 2.62-2.58 (m, 1H), 2.07-2.04 (m, 1H), 1.90-1.86 (m, 1H), 1.72-1.68 (m, 2H), 1.54-1.52 (m, 1H) and 1.23-1.20 (m, 1H).

LCMS= [M+H] ⁺ : (672.56), Purity = 96%

Synthesis of (R,E)-4-((3-(4-(azetidin-l-yl)but-2-enamido)piperidin-l-yl)m ethyl)-N-(4-(4-methyl- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (106):

[00628] To an ice cold stirred solution of (R)-4-((3-aminopiperi din- l-yl)methyl)-N-(4-(4-m ethyl - 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide 7 (150 mg, 340 mmol) in DMF (1.00 mL), were added (E)-4-(azetidin-l-yl)but-2-enoic acid RG3 (144 mg, 1.02 mmol), DIPEA (0.3 mL, 1.70 mmol) and T3P (50% in ethyl acetate; 0.65 mL, 1.02 mmol) in drop wise manner. The resulting reaction mixture was stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mass was evaporated to dryness and the crude residue was purified through RP-HPLC purification using eluent as 5mM Ammonium Bicarbonate in water/ Acetonitrile and column using Waters Xselect Phenyl-Hexyl(19*250mm,5pm to get desired product as an off-white solid 106 (26 mg, 13% yield).

¹ H NMR (400 MHz, DMSO-t/ ₆ ): 12.4 (s, 1H), 10.80 (s, 1H), 8.69-8.68 (m, 1H), 8.59 (s, 1H), 8.11 (s, 1H), 8.06-8.03 (d, J= 8.8 Hz, 2H), 7.97-7.95 (d, J= 8.8 Hz, 2H), 7.87-7.85 (d, J= 8.0 Hz, 1H), 7.63- 7.62 (m, 1H), 7.09(s, 1H), 6.45- 6.38 (m, 1H), 6.01-5.97 (m, 1H), 3.80-3.79(m, 1 H), 3.65 (s, 1H), 3.11-3.05 (m, 5H), 2.77-2.75 (m, 2H), 2.66-2.63 (m, 5H), 2.06-2.03 (m, 1H), 2.01-1.98 (m, 3H), 1.75- 1.72 (m, 2H), 1.57-1.48 (m, 1H) and 1.20-1.15 (m, 1H). LCMS= [M+H]+: (565.30), Purity = 95%. wherein A, R ⁷ , R ^6b , R ^6c , and p are as described herein, and PG is a protecting group as described herein.

wherein A, R ⁷ , R ^6a , R ^6b , R ^6c , and p are as described herein.

Example B0600-1

Synthesis of Compound B0600-201

(R)-4-((3-(2-fluoroacrylamido)piperidin-l-yl)methyl)-N-(4 -(4-morpholino-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide

[00629] To a stirred solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide 10 (400 mg, 0.780 mmol) and 2-fluoroacrylic acid (84 mg, 0.936 mmol) in DMSO (8.0 mL) was added DIPEA (0.69 mL, 3.90 mmol) followed by addition of HATU (556 mg, 1.463 mmol) at 10°C. The resulting reaction mixture was stirred at room temperature for next Ih. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice cold water (80 mL), solid precipitation observed. Solid thus obtained was filtered through buchner funnel and washed with cold water (2 x 20 mL). The purity of solid material was close to 90% by LCMS which was further purified by flash column chromatography (silica gel, 12 g SNAP) using 1-2% MeOH in DCM as eluent to get the desired product as light yellow solid 201 (235 mg, 52%). ^X H NMR (400 MHz, DMSO-d6): 12.19 (s, 1H), 10.73 (s, 1H), 8.69-8.68 (d, J= 4.80 Hz, 1H), 8.27-8.25 (d, J= 8.40 Hz, 1H), 8.18 (s, 1H), 8.11 (s, 1H), 8.01-7.98 (m, 2H), 7.92-7.90 (m, 2H), 7.62-7.60 (d, J= 4.40 Hz, 1H), 7.16 (s, 1H), 5.56-5.43 (dd, J= 3.20 Hz, 1H), 5.25-5.20 (dd, J= 3.20 Hz, 1H), 3.90-3.87 (m, 5H), 3.76-3.74 (m, 4H), 3.66 (s, 2H), 2.78-2.76 (m, 1H), 2.70-2.66 (m, 1H), 2.01-1.96 (m, 2H), 1.75-1.66 (m, 2H), 1.58-1.48 (m, 1H) and 1.40-1.34 (m, 1H). LCMS= [M+H]+: (585.16), Purity= 97.50%.

B0700 Compounds

Example B0700-1

Synthesis of Compound B0700-301

[00630] Preparation of (R)-4-((3-(N-methylacrylamido)piperidin-l-yl)methyl)-N-(4-(4 -morpholino- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (Compound B0700-301):

Synthesis of tert-butyl (R)-(l-((2-cyanopyridin-4-yl)methyl)piperidin-3-yl)(methyl)c arbamate (32):

[00631] To an ice cold stirred solution of 4-(bromomethyl)picolinonitrile 17 (2 g, 10.15 mmol) in DCM (20 mL) was added tert-butyl (R)-methyl(piperidin-3-yl)carbamate 31 (2.60 g, 12.18 mmol) and DIPEA (5.40 mL, 30.45 mmol) at room temperature. The resulting reaction mass was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with DCM (50 mL) and washed with ice-cold water (2 x 25 mL). The combined organic layer was washed with brine solution (100 mL), dried over Na2SO4, filtered and evaporated under reduced pressure to get crude residue, which was purified over flash column chromatography (silica gel, 12 g SNAP) using eluent as 40% ethyl acetate in heptane to get the desired product as viscous liquid 32 (1.05 g). LCMS= [M+H]+: 331.33, Purity= 80.06%

Synthesis of (R)-4-((3-((tert-butoxycarbonyl)(methyl)amino)piperidin-l-yl )methyl)picolinic acid (33):

[00632] To an ice-cold stirred solution of tert-butyl (R)-(l-((2-cyanopyridin-4-yl)methyl)piperidin- 3-yl)(methyl)carbamate 32 (1 g, 3.03 mmol) in 1,4-dioxane (10 mL) was added LiOH.PLO (1.28 g, 30.26 mmol, dissolved in 4 mL H2O) in drop wise manner. The resulting reaction mixture was stirred at 80°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure to get crude residue which was diluted with water (25 mL) and aqueous layer was washed with EtOAc (2 x 50 mL) which was discarded. The pH of aqueous layer was bought up to ~ 4-6 using 6N HC1 followed by extraction of compound using 20% IPA: CHCL (4 x 50 mL) and concentrated under reduced pressure to get the desired product as off-white solid 33 (750 mg, 72%).

LCMS= [M+H] ⁺ : 350.02, Purity= 80.69%.

Synthesis of tert-butyl (R)-methyl(l-((2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidi n-6- yl)phenyl)carbamoyl)pyridin-4-yl)methyl)piperidin-3-yl)carba mate (34):

[00633] To a stirred solution of (R)-4-((3-((tert-butoxycarbonyl)(methyl)amino)piperidin-l- yl)methyl)picolinic acid 33 (250 mg, 0.715 mmol) and 4-(4-morpholino-7H-pyrrolo[2,3- d]pyrimidin-6-yl)aniline 7 (253 mg, 0.858 mmol) in DMF (10 mL) was added EtsN (0.298 mL, 2.15 mmol) and HATU (407 mg, 1.07 mmol) at room temperature. The resulting reaction mixture was stirred at room temperature for next 16h. After completion of reaction (TLC monitoring), the resulting reaction mixture was poured into ice-cold water (20 mL) and extracted with ethyl acetate (3 x 15 mL). The combined organic layer was washed with brine solution (3 x 25 mL), dried over Na2SO4 filtered and evaporated under reduced pressure to get the crude residue. The crude was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 5% MeOH in DCM to get the desired product as viscous liquid 34 (150 mg, 33%).

LCMS= [M+H] ⁺ : 627.25, Purity= 82%. Synthesis of (R)-4-((3-(methylamino)piperidin-l-yl)methyl)-N-(4-(4-morpho lino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (35):

[00634] To an ice cold stirred solution of tert-butyl (R)-methyl(l-((2-((4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)m ethyl)piperidin-3-yl)carbamate 34 (90 mg, 0.143 mmol) in DCM (2 mL) was added TFA (0.50 mL, diluted in 2 mL of DCM) in drop wise manner. The resulting reaction mixture was stirred at room temperature for 2h. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure to get the crude residue as TFA salt which was neutralized with Aq. NFUOH extracted with 10% MeOH in DCM (3 x 10 mL). The combined organic layer was dried over lSfeSCU, filtered and solvnet evaporated under reduced pressure to get the crude residue. The crude so obtained was purified over column chromatography (100-200 mesh silica gel) eluted with 15% MeOH in DCM to get the desired product as yellow solid 35 (70.0 mg, 92%).

'H NMR (400 MHz, DMSO-d6): 12.20 (s, 1H), 10.75 (s, 1H), 8.70 (d, J= 4.8 Hz, 1H), 8.18 (d, J= 13.6 Hz, 2H), 8.01 (d, J= 8.0 Hz, 2H), 7.92 (d, J= 8.0 Hz, 2H), 7.64 (s, 1H), 7.16 (s, 1H), 3.88 (m, 4H), 3.75 (m, 4H), 3.70 (s, 2H), 3.07 (m, 1H), 2.32-2.28 (m, 2H), 1.90-1.73 (m, 4H) and 1.50-1.23 (m, 6H). LCMS= [M+H]+: (527.41), Purity= 89.17%

Synthesis of (R)-4-((3-(N-methylacrylamido)piperidin-l-yl)methyl)-N-(4-(4 -morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (Compound B0700-1):

[00635] To an ice cold stirred solution of (R)-4-((3-(methylamino)piperidin-l-yl)methyl)-N-(4-(4- morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinami de 35 (70 mg, 0.133 mmol) in DMF (2 mL) was added EtsN (67.30 mg, 0.665 mmol) and Acryloyl chloride 27 (10.8 pL, 0.133 mmol). The resulting reaction mixture was stirred at room temperature for next 15 minutes. After completion of reaction (TLC and LCMS monitoring), the reaction mass was directly purified by RP- HPLC using 5mM Ammonium Bicarbonate in water/ Acetonitrile (column: Waters Xselect Phenyl- Hexyl(19*250mm, 5 pm) to get desired product as off white solid 301 (17 mg, 22%).

'H NMR (400 MHz, DMSO-t/ ₆ : 12.20 (s, 1H), 10.74 (s, 1H), 8.68 (d, J= 4.8 Hz, 1H), 8.18 (s, 1H), 8.11 (s, 1H), 8.01 (d, J= 8.4 Hz, 2H), 7.92 (d, J= 8.8 Hz, 2H), 7.62 (d, J = 4.0 Hz, 1H), 7.16 (s, 1H),

6.76- 6.66 (m, 1H), 6.09-5.99 (m, 1H), 5.65 (d, J= 9.6 Hz, 1H), 4.47 (br, s, 1H), 3.88-3.87 (m, 4H),

3.76-3.75 (m, 4H), 3.68-3.66 (m, 2H), 2.90 (s, 3H), 2.76-2.64 (m, 2H), 2.24-2.08 (m, 2H) and 1.72- 1.49 (m, 4H). LCMS= [M+H] ⁺ : (581.20), Purity= 99.61%. [00636] The following compound was or can be prepared using the appropriate reagents and the corresponding starting materials, and following the procedure described in Example B0700-1 :

Example B0700-1A

Synthesis of Compound B0700-324

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(6-(4-morpholin o-7H- pyrrolo [2, 3-d] pyrimidin-6-yl)pyridin-3-yl)picolinam336 ide (324):

Synthesis of 4-(6-(5-chloropyridin-2-yl)-7-((2-(trimethylsilyl)ethoxy)met hyl)-7H-pyrrolo [2,3- d]pyrimidin-4-yl)morpholine (134):

[00637] To a stirred solution of 4-(6-iodo-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2, 3- d]pyrimidin-4-yl)morpholine 5 (3.0 g, 6.52 mmol) in N,N-dimethylformamide (45 mL) was added (5- chloropyridin-2-yl)boronic acid 133 (4.10 g, 26.1 mmol) and CS2CO3 (4.25 g, 13.0 mmol). The resulting reaction mixture was degassed with argon for 15 minutes followed by addition of Cui (1.24 g, 6.52 mmol), palladium acetate (73.1 mg, 326 pmol) and dppf (361 mg, 652 pmol). The resulting reaction mixture was stirred at 100°C for 16h. After the completion of reaction (TLC and LCMS monitoring), the reaction mixture was filtered through celite bed and washed with ethyl acetate (50 mL). The organic layer was diluted with water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 120 g SNAP) elution with 60% EtOAc in heptane to get the desired product as brown liquid 134 (1.40 g, 48%). LCMS: [M+H] ⁺ : 446.22 Purity= 60%.

Synthesis of tert-butyl (6-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyr rolo[2,3- d]pyrimidin-6-yl)pyridin-3-yl)carbamate (136):

[00638] To a stirred solution of 4-[6-(5-chloropyridin-2-yl)-7-{[2-(trimethylsilyl)ethoxy]met hyl}- 7H-pyrrolo[2,3-d]pyrimidin-4-yl]morpholine 134 (1.20 g, 2.69 mmol) in 1,4-dioxane (20 mL) was added CS ₂ CO ₃ (1.75 g, 5.38 mmol) and tert-butyl carbamate 135 (946 mg, 8.07 mmol). The reaction mixture was degassed with argon for 15 minutes followed by addition of Pd(OAc)2 (36.2 mg, 161 pmol) and X-phos (256 mg, 538 pmol). The resulting reaction mixture was stirred at 100°C for 16h. After the completion of reaction (TLC and LCMS monitoring), the reaction mixture was filtered through celite bed, washed with ethyl acetate (50 mL). The organic layer diluted with water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer washed with brine solution dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified over flash column chromatography (silica gel, 120 g SNAP) eluted with 2% MeOH in DCM to get the desired product as colourless liquid 136 (900 mg, 63%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 5 9.72 (s, 1H), 8.66 (s, 1H), 8.26 (s, 1H), 8.00 (d, J= 4.0 Hz, 1H), 7.88-7.86 (m, 1H), 7.22 (s, 1H), 6.09 (s, 2H), 3.91-3.89 (m, 4H), 3.75-3.73 (m, 4H), 3.36-3.34 (m, 2H), 1.49 (s, 9H), 0.70-0.66 (m, 2H) and 0.21 (s, 9H).

Synthesis of 6-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyrr olo[2,3-d]pyrimidin- 6-yl)pyridin-3-amine (137):

[00639] To an ice-cold solution of tert-butyl N-{6-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]pyridin-3-yl (carbamate 136 (900 mg, 1.71 mmol) in DCM (20.0 mL) was added p-TSA (1.18 g, 6.83 mmol) portion wise. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), reaction mixture was diluted with saturated solution of NaHCOs and extracted with DCM (3 x 100 mL). The combined organic layer washed with brine solution (3 x 100 mL), dried over Na2SO4, filtered and solvent was evaporated under reduced pressure to get the get desired product as light blackish solid 137 (700 mg, 96%). 'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.23 (s, 1H), 8.0 (s, 1H), 7.62-7.60 (m, 1H), 7.02-6.99 (m, 2H), 6.05 (s, 1H), 5.59 (br s, 1H), 6.09 (s, 2H), 3.89-3.88 (m, 4H), 3.75-3.74 (m, 4H), 3.40-3.37 (m, 2H), 0.71-0.67 (m, 2H) and 0.18 (s, 9H). LCMS: [M+H] ⁺ : 427.29, Purity= 81%.

Synthesis of tert-butyl (R)-(l-((2-((6-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)me thyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)pyridin-3-yl)carbamoyl)pyridin- 4-yl)methyl)piperidin-3- yl)carbamate (138):

[00640] To a stirred solution of 4-{[(3R)-3-{[(tert-butoxy)carbonyl]amino}piperidin-l- yl]methyl}pyridine-2-carboxylic acid 8 (826 mg, 2.46 mmol) in DMF (15 mL) was added 6-[4- (morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyr rolo[2,3-d]pyrimidin-6-yl]pyri din-3- amine 137 (700 mg, 1.64 mmol), DIPEA (1.43 mL, 8.20 mmol) and HATU (936 mg, 2.46 mmol). The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting reaction mass was diluted with ice-cold water (20 mL) and extracted with ethyl acetate (3 x 150 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and solvent was evaporated under reduced pressure. The crude was purified over flash column chromatography (silica gel, 40 g SNAP) eluted with 50% ethyl acetate in heptane to get desired product as light brown solid 138 (700 mg, 57%). LCMS [M+H] ⁺ : 743.99, Purity= 70%

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(6-(4-morpholino-7H- pyrrolo[2,3- d]pyrimidin-6-yl)pyridin-3-yl)picolinamide (139):

[00641] To an ice cold solution of tert-butyl N-[(3R)-l-{[2-({6-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]pyridin-3-yl}carbamoyl)pyri din-4- yl]methyl}piperi din-3 -yl]carbamate 138 (700 mg, 941 pmol) in DCM (10 mL) was added TFA (5 mL) in drop wise. The resulting reaction mixture was allowed to stirred at room temperature for 24h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude residue was triturated with diethyl ether to get desired product 139 as brown sticky solid (600 mg, quantitative). LCMS: [M+H] ⁺ : 514.35, Purity= 70%.

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(6-(4-morpholin o-7H-pyrrolo[2,3- d]pyrimidin-6-yl)pyridin-3-yl)picolinamide (324):

[00642] To an ice cold stirred solution of 4-{[(3R)-3-aminopiperidin-l-yl]methyl}-N-{6-[4- (morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]pyridin-3-y l}pyridine-2-carboxamide 139 (370 mg, 720 pmol) in DMF (5 mL) was added EtsN (0.25 mL, 2.16 mmol) and acryloyl chloride 27 (0.08 mL, 792 pmol) in drop wise. The resulting reaction mixture was stirred at 0°C for 30 min. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was evaporated up to complete dryness and the crude residue was purified through RP-HPLC purification using eluent as 5mM ammonium bicarbonate in water/acetonitrile and column using Waters Xselect Phenyl-Hexyl(19*250mm,5pm to get desired product as white solid 324 (8.0 mg, 2%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 5 12.31 (s, 1H), 11.03 (s, 1H), 9.15 (d, J = 4.0 Hz 1H), 8.70 (d, J = 4.0 Hz, 1H), 8.42-8.39 (m, 1H), 8.19 (s, 1H), 8.12 (s, 1H), 8.04-7.99 (m, 2H), 7.65-7.64 (m, 1H), 7.35 (s, 1H), 6.25-6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57-5.54 (m, 1H), 3.91-3.89 (m, 4H), 3.82-3.81 (m, 1H), 3.77-3.74 (m, 4H), 3.64 (s, 1H), 2.79-2.77 (m, 1H), 2.66-2.64 (m, 2H) 2.06-2.01 (m, 1H), 1.91- 1.86 (m, 1H), 1.77-1.65 (m, 2H), 1.59-1.52 (m, 1H) and 1.25-1.23 (m, 1H).

LCMS: [M+H] ⁺ : 568.42, Purity= 99%.

Example B0700-2

Synthesis of Compound B0700-302

[00643] Preparation of 4-((l-acryloylpiperidin-3-yl)amino)-N-(4-(4-morpholino-7H-py rrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide (Compound B0700-302):

Synthesis of 4-((l-(tert-butoxycarbonyl)piperidin-3-yl)amino)picolinic acid (38):

To a stirred solution of methyl 4-chloropicolinate 36 (1.0 g, 5.83 mmol) in toluene (20 mL) was added tert-butyl 3 -aminopiperidine- 1 -carboxylate 37 (2.33 g, 11.7 mmol) and CS2CO3 (5.70 g, 17.50 mmol) at room temperature. The resulting reaction mixture was degassed with argon for next 15 minutes followed by addition of Pd(OAc)2 (262 mg, 1.17 mmol) and Xantphos (674 mg, 1.17 mmol). The resulting reaction mixture was stirred at 100° C for next 16h. After the completion of reaction (TLC and LCMS monitoring), the reaction mixture was filtered through celite bed followed by washing with ethyl acetate (50 mL). Mother liquor was diluted with water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (2 x 100 mL), dried over ISfeSCU filtered and evaporated under reduced pressure to get crude residue, which was purified by flash column chromatography (silicagel,120 g SNAP) using eluents 2% MeOH in DCM to get the desired product as beige coloured gummy solid 38 (400 mg, 21%). LCMS= [M+H] ⁺ : 322.17, Purity = 81%.

Synthesis of tert-butyl 3-((2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl) -7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)a mino)piperidine-l-carboxylate (39):

To a stirred solution of 4-((l-(tert-butoxycarbonyl)piperidin-3-yl)amino)picolinic acid 38 (400 mg, 1.25 mmol) in DMF (10 mL) was added 4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)aniline 7 (528 mg, 1.25 mmol) followed by the addition of DIPEA (1.30 mL, 7.46 mmol) and HATU (709 mg, 1.87 mmol) at room temperature. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (20 mL) and extracted with ethyl acetate (3 x 150 mL). The combined organic layer was washed with brine solution (3 x 100 mL), dried over Na2SO4, filtered and solvent was evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 50% ethyl acetate in heptane to get desired product as gummy liquid 39 (250 mg, 28%). LCMS [M+H]+: 729.38, Purity = 65%

Synthesis of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-4 -(piperidin-3- ylamino)picolinamide (40):

[00644] To an ice cold solution of tert-butyl 3-((2-((4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyri din-4- yl)amino)piperidine-l -carboxylate 39 (250 mg, 0.343 mmol) in DCM (16 mL), was added TFA (4 mL) in drop wise manner. The resulting reaction mixture was allowed to stir at room temperature for 24h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue which was triturated with di-ethyl ether (30 mL). The sticky solids so obtained was treated with Aq NH4OH solution, solid precipitated out, filtered off and washed with water (2 x 25 mL) to get the desired product as yellow solid 40 (150 mg, 88%).

¹ H NMR (400 MHz, DMSO-d6): 12.18 (s, 1H), 10.58 (s, 1H), 8.20-8.18 (m, 2H), 8.27 (s, 1H), 7.93- 7.87 (m, 4H), 7.43-7.42 (d, J= 4.0 Hz, 1H), 7.05 (s, 1H), 6.78-6.76 (m, 3H), 3.91-3.89 (m, 4H), 3.78-3.76 (m, 4H), 3.51 (br s, 1H), 3.24-3.18 (m, 1H), 2.05 (s, 1H), 1.76 (s, 1H) and 1.55-1.45 (m, 2H). LCMS= [M+H] ⁺ : 499.25, Purity= 92%.

Synthesis of 4-((l-acryloylpiperidin-3-yl)amino)-N-(4-(4-morpholino-7H-py rrolo [2,3- d]pyrimidin-6-yl)phenyl)picolinamide (Compound B0700-2):

[00645] To an ice cold stirred solution of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-4-(piperidin-3-ylamino)picolinamide 40 (150 mg, 0.30 mmol) in DMF (5 mL), was added EtsN (0.13 mL, 0.90 mmol) and acryloyl chloride 27 (32 pL, 0.36 mmol) in drop wise manner. The resulting reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was evaporated up to complete dryness and the crude residue was purified through RP-HPLC purification using 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column: Waters Xselect Phenyl-Hexyl(19*250mm,5pm) to get desired product as off-white solid Compound B0700-302 (50 mg, Yield: 30%).

¹ H NMR (400 MHz, DMSO-t/ ₆ : 12.18 (s, 1H), 10.58 (s, 1H), 8.20 (m, 2H), 7.98 (d, 8.40 Hz,

1H), 7.90 (d, 8.80 Hz, 1H), 7.40 (s, 1H), 7.15 (s, 1H), 6.99 (d, J= 7.60 Hz, 1H), 6.87-6.60 (m,

2H), 6.16-6.02 (m, 1H), 5.71-5.58 (m, 1H), 4.39 (m, 1H), 3.91-3.89 (m, 6H), 3.78-3.76 (m, 5H), 3.51 (s, 1H), 3.24-3.18 (m, 1H), 2.68 (m, 1H), 2.01 (s, 1H), 1.81(m, 1H) and 1.52-1.50 (m, 2H). LCMS= [M+H] ⁺ : 553.34, Purity= 96.81%.

Example B0700-3

Synthesis of Compound B0700-303

[00646] Preparation of 4-(2-(4-acryloylpiperazin-l-yl)ethyl)-N-(4-(4-morpholino-7H- pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)picolinamide (Compound B0700-303): Synthesis of methyl 4-vinylpicolinate (42):

[00647] To a stirred solution of methyl 4-bromopyridine-2-carboxylate 41 (5.00 g, 23.10 mmol) in THF (60 mL) was added Tributyl vinyl tin (14.90 mL, 50.90 mmol) and triphenylphosphine (1.82 g, 6.94 mmol) follwed by the addition of Pd(OAc)2 (779 mg, 3.47 mmol) under inert and anhydrous atmosphere. The resulting reaction reaction mixture was stirred at 80 °C for 6h. After completion of reaction ( TLC monitoring), solvent was evaported under reduced pressure to get the crude residue which was diluted with water (100 mL) and extracted with EtOAc (2 x 200 mL). Combined organic layer was washed with brine (100 mL), dried over anhydrous Na2SO4, filteretd and solvent was evaporated under reduced pressure to get the residue which was purified by flash silica-gel column chromatography (5-15% EtOAc/Heptane) to get desired product as a yellow liquid 42 (2.00 g, 53%). 'H NMR (400 MHz, DMSO-t/ ₆ ) 8.67-8.65 (d, J= 8.0 Hz, 1H), 8.08 (s, 1H), 7.73-7.72 (d, J= 4.0 Hz, 1H), 6.26-6.21 (d, J= 20.0 Hz, 1H), 5.62-5.60 (d, J= 8.0 Hz, 1H), 3.88 (s, 3H).

LCMS = [M+H] ⁺ : 164.06, Purity = 93%.

Synthesis of tert-butyl 4-(2-(2-(methoxycarbonyl)pyridin-4-yl)ethyl)piperazine-l-car boxylate (44):

[00648] To a stirred solution of tert-butyl piperazine- 1 -carboxylate 43 (2.28 g, 12.30 mmol) in ethanol (20 mL) added DIPEA (6.42 mL, 36.80 mmol) and methyl 4-ethenylpyridine-2-carboxylate 42 (2.00 g, 12.30 mmol) and raised the temarature to 120°C for 16 h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue which was diluted with water (50 mL) and extracted with EtOAc (2 x 100 mL). The combined organic layer was washed with brine (100 mL), dried over anhydrous Na2SO4, filtered and solvent was evaporated under reduced pressure to get crude residue which was purified by silica-gel column chromatography eluted with 5-40% EtOAc in Heptane to get desired product tert-butyl 4-{2- [2-(methoxycarbonyl)pyridin-4-yl]ethyl [piperazine- 1 -carboxylate as yellow gel 44 (1.10 g, 29%).

^X H NMR (400 MHz, DMSO-d6) 8.58 (d, J= 4.0 Hz, 1H), 7.94 (s, 1H), 7.53 (d, J= 4.0 Hz, 1H), 3.87 (s, 3H), 3.46(m, 4H), 2.87-2.83 (m, 2H), 2.59-256 (m, 2H), 2.22 (m, 4H), 1.38 (s, 9H).

LCMS = [M+H] ⁺ : 350.20, Purity = 89.7%.

Synthesis of 4-(2-(4-(tert-butoxycarbonyl)piperazin-l-yl)ethyl)picolinic acid (45):

[00649] To a stirred solution of tert-butyl 4-{2-[2-(methoxycarbonyl)pyridin-4-yl]ethyl[piperazine- 1-carboxylate 44 (500 mg, 1.43 mmol) in THF (5.00 mL) added lithium hydroxide (171 mg, 7.15 mmol) in water (5.00 mL) at 0 °C. The resultant reaction mixture was stirred at room temperature for 4 h. After completion of reaction (TLC Monitoring), the reaction mixture was diluted with water (10 mL) and acidified with cone, acetic acid up to pH 5 then extracted with 15% MeOH:DCM (4 x 50 mL). The combined organic layer was washed with brine (20 mL), dried over anhydrous TsfeSCh, concentrated under reduced pressure to get 4-(2-{4-[(tert-butoxy)carbonyl]piperazin-l- yl}ethyl)pyridine-2-carboxylic acid as a brown oil 45 (400 mg, 83%).

¹ H NMR (400 MHz, DMSO-d6) 11.73 (s, 1H), 8.58-857 (d, J= 4.0 Hz, 1H), 7.94 (s, 1H), 7.53-7.52 (d, J= 4.0 Hz, 1H), 3.46 (m, 4H), 2.87-2.83 (m, 2H), 2.59-256 (m, 2H), 2.22 (m, 4H) and 1.38 (s, 9H). LCMS= [M+H] ⁺ : 336.18, Purity= 98%.

Synthesis of tert-butyl 4-(2-(2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methy l)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)e thyl)piperazine-l-carboxylate (46):

[00650] To a stirred solution of 4-(2-(4-(tert-butoxycarbonyl)piperazin-l-yl)ethyl)picolinic acid 45 (300 mg, 0.89 mmol) in DMF (5 mL) was added 4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)aniline 7 (266 mg, 0.626 mmol) and DIPEA (0.781 mL, 4.47 mmol) followed by the addition of HATU (316 mg, 1.34 mmol) at room temperature. The resulting reaction mixture was stirred at same temperature for next 16h. After completion of reaction (TLC monitoring), reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed brine solution (3 x 50 mL), dried over ISfeSCL, filtered and solvent was evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 40% ethyl acetate in heptane to get the desired product as gummy solid 46 (170 mg, 25%).

'H NMR (400 MHz, DMSO-d6): 10.79 (s, 1H), 8.63-8.62 (d, J= 8.0 Hz, 1H), 8.10 (s, 1H), 8.08 (s, 1H), 7.96-7.94 (d, J = 8.0 Hz, 2H), 7.90-7.88 (d, J = 8.0 Hz, 2H), 7.56-7.55 (d, J = 4.0 Hz, 1H), 7.06 (s, 1H), 6.76- 6.69 (m, 1H), 3.88-3.86 (m, 4H), 3.75-3.72 (m, 4H), 3.51 (m, 4H), 2.92-2.89 (t, 2H), 2.65-2.62 (t, 2H), 2.40 (m, 4H), 1.39 (s, 9H), 0.88 (t, 4H) and 0.07 (s, 9H).

LCMS = [M+H] ⁺ : 744.0, Purity = 90%

Synthesis of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-4 -(2-(piperazin-l- yl)ethyl)picolinamide (47):

To an ice cold solution of tert-butyl 4-(2-(2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methy l)- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-y l)ethyl)piperazine-l-carboxylate 46 (170 mg, 0.23 mmol) in DCM (5 mL) was added TFA (5.0 mL) in drop wise manner. The resulting reaction mixture was allowed to stir at room temperature for 24h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue which was triturated with di-ethyl ether (50 mL). The sticky solids so obtained was treated with NH4OH solution, and washed with water (2 x 25 mL) to get the desired product as brown solid (90 mg, 76%). LCMS= [M+H] ⁺ : 513.26, Purity= 90%.

Synthesis of 4-(2-(4-acryloylpiperazin-l-yl)ethyl)-N-(4-(4-morpholino-7H- pyrrolo [2,3- d]pyrimidin-6-yl)phenyl)picolinamide (Compound B0700-303):

[00651] To an ice cold stirred solution of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-4-(2-(piperazin-l-yl)ethyl)picolinamide 47 (90 mg, 0.18 mmol) in DMF (5.0 mL) was added EtsN (0.07 mL, 0.527 mmol) and acryloyl chloride 27 (18 pL, 0.210 mmol) in drop wise manner. The resulting reaction mixture was stirred at same temperature for next 30 minutes. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure up to dryness to get crude residue. The crude was purified through RP-HPLC purification using 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column: Waters Xselect Phenyl- Hexyl(19*250mm,5pm) to get desired product as white solid Compound B0700-3 (22 mg, 22%).

'H NMR (400 MHz, DMSO-</ ₆ : 12.20 (s, 1H), 10.72 (s, 1H), 8.63 (d, J= 8.0 Hz, 1H), 8.18 (s, 1H), 8.08 (s, 1H), 7.96-7.94 (d, J = 8.0 Hz, 2H), 7.90-7.88 (d, J = 8.0 Hz, 2H), 7.59-7.58 (d, J = 4.0 Hz, 1H), 7.16 (s, 1H), 6.83- 6.77 (m, 1H), 6.12-6.08 (m, 1H), 5.69-5.65 (m, 1H), 3.88-3.86 (m, 4H), 3.75-3.72 (m, 4H), 3.53 (m, 4H), 2.94-2.91(t, 2H), 2.66-2.65 (t, 2H) and 2.45 (m, 4H).

LCMS= [M+H] ⁺ : 567.29, Purity= 99.70%.

Example B0700-4

Synthesis of Compound B0700-308

[00652] Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(l-(6-morpholin o-9H-purin-8- yl)piperidin-4-yl)picolinamide (Compound B0700-308):

Synthesis of 4-(9H-purin-6-yl)morpholine (49):

[00653] To a stirred solution of 6-Chloro-9H-Purine 48 (1.80 g, 11.6 mmol) in n-BuOH (25 mL) was added DIPEA (6.20 mL, 34.90 mmol) and morpholine (3.0 mL, 34.90 mmol) sequentially at room temperature. The resulting reaction mixture was stirred at 90°C for 4h. After completion of reaction (TLC monitoring), the reaction mixture was cooled to room temperature quenched with ice cold- water (90 mL), the slurry was stirred at room temperature for Ih then the precipitate was collected by filtration and dried under reduced pressure to get the desired product as Off white solid 49 (2.10 g, 88%).

'H NMR (400 MHz, DMSO-d6): 13.07 (s, IH), 8.22 (s, IH), 8.13 (s, IH), 4.20 (m, 4H) and 3.72- 3.69 (m, 4H). LCMS = [M+H] ^+: 206.10, Purity = 99.90%.

Synthesis of 4-(8-bromo-9H-purin-6-yl)morpholine (50):

[00654] To a stirred solution of 4-(9H-purin-6-yl)morpholine 49 (2.10 g, 10.20 mmol) in ACN (40 mL) added NBS (2.19 g, 12.30 mmol) and stirred at 60°C for 4h. After completion of reaction (TLC monitoring), the reaction mixture was cooled to room temperature diluted with ice cold-water (90 mL), the slurry was stirred at room temperature for Ih, the precipitate was collected by filtration and dried under reduced pressure to get the desired product as pale yellow solid 50 (1.70 g, 59%). 'H NMR (400 MHz, DMSO-d6): 13.91 (s, 1H), 8.23 (s, 1H), 4.14 (m, 4H), 3.71 (m, 4H). LCMS= [M+H] ⁺ : 284.01, Purity = 95%.

Synthesis of 4-(8-bromo-9-((2-(trimethylsilyl)ethoxy)methyl)-9H-purin-6-y l)morpholine (51): [00655] To an ice cold stirred solution of 4-(8-bromo-9H-purin-6-yl)morpholine 50 (1.70 g, 5.98 mmol) in DMF (20 mL), added NaH (0.36 g, 8.97 mmol) in portion wise manner and stirred for 30 minutes at same temperature followed by addition of SEM-C1 (1.27 mL, 7.17 mmol). The resulting reaction mixture was stirred at RT for 2h. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice-cold water (100 mL) followed by extraction with EtOAc (2 x 200 mL). The combined organic layer was washed with brine solution (2 x 50 mL), dried over Na2SO4, filtered and solvent was evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40g SNAP) using eluent 40% ethyl acetate in heptane to get the desired product as gummy solid 51 (mix of regio-isomers; 2.0 g, 81%).

^X H NMR (400 MHz, DMSO-d6): 8.50 (s, 1H), 5.41 (s, 1H), 4.14 (m, 4H ), 3.70 (m, 5H), 3.23 (t, 2H), 0.87 (t, 2H), 0.08 (s, 9H). LCMS= [M+H] ⁺ : 414.01, Purity= 97%.

Synthesis of tert-butyl (l-(6-morpholino-9-((2-(trimethylsilyl)ethoxy)methyl)-9H-pur in-8- yl)piperidin-4-yl)carbamate (53):

[00656] To a stirred solution of 4-(8-bromo-9-((2-(trimethylsilyl)ethoxy)methyl)-9H-purin-6- yl)morpholine 51 (2.0 g, 4.83 mmol) in 1,4-dioxane (20 mL) was added tert-butyl piperidin-4- ylcarbamate 52 (1.45 g, 7.24 mmol) and CS2CO3 (4.72 g, 14.49 mmol) at room temperature. The resulting reaction mixture was degassed with argon for 15 minutes followed by addition of Pd2(dba)3 (0.44 g, 0.48 mmol) and Xantphos (0.56 g, 0.97 mmol) at room temperature. The resulting reaction mixture was stirred at 100°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed followed by washing with ethyl acetate (50 mL). The reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (2 x 50 mL), dried over Na2SO4, filtered and evaporated under reduced pressure to get crude residue, which was purified by flash column chromatography (silicagel,120 g SNAP) eluted with 10% ethyl acetate in heptane to get desired product as gummy solid 53 (1.0 g, 39%).

'H NMR (400 MHz, DMSO-d6): 8.23 (s, 1H), 7.32 (s, 1H), 5.58 (s, 2H), 4.30 (m, 2H), 4.10 (m, 4H), 3.70-7.30 (m, 4H), 3.41 (m, 1H), 3.23 (m, 2H), 2.90-2.84 (m, 2H), 1.75-1.72 (m, 2H), 1.38 (s, 9H), 0.09 (m, 2H) and 0.05 (s, 9H). LCMS= [M+H] ⁺ : 534.31, Purity= 80%. Synthesis of l-(6-morpholino-9H-purin-8-yl)piperidin-4-amine (54):

[00657] To an ice cold solution of tert-butyl (l-(6-morpholino-9-((2-(trimethylsilyl)ethoxy)methyl)- 9H-purin-8-yl)piperidin-4-yl)carbamate 53 (1.0 g, 446 mmol) in DCM (5 mL), was added TFA (5 mL). The resulting reaction mixture was allowed to stir at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue, which was neutralized with Aq. NH4OH solution, the solid so precipitated was collected by filtration and washed with water (2 x 25 mL) to get the desired product as brown solid 54 (500 mg, 87%).

'H NMR (400 MHz, DMSO-d6 ): 8.02 (s, 1H), 4.26-4.10 (m, 4H), 3.77-3.67 (m, 4H), 4.10-3.96 (m, 1H), 3.12-2.66 (m, 4H) and 1.78-1.16 (m, 4H). LCMS= [M+H] ⁺ : (304.18), Purity= 80% Synthesis of tert-butyl (R)-(l-((2-((l-(6-morpholino-9H-purin-8-yl)piperidin-4- yl)carbamoyl)pyridin-4-yl)methyl)piperidin-3-yl)carbamate (55):

To a stirred solution of l-(6-morpholino-9H-purin-8-yl)piperidin-4-amine 54 (500 mg, 1.67 mmol) in DMF (10 mL) was added (R)-4-((3-((tert-butoxycarbonyl)amino)piperidin-l-yl)methyl) picolinic acid 8 (662 mg, 1.98 mmol) followed by the addition of DIPEA (0.88 mL, 4.94 mmol) and HATU (983 mg, 2.47 mmol) at room temperature. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organics was washed brine solution (3 x 50 mL), dried over ISfeSCU, filtered and evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 50% ethyl acetate in heptane to get the desired product as viscous liquid 55 (400 mg, 40%). LCMS= [M+H] ⁺ : 621.35, Purity= 60%.

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(l-(6-morpholino-9H- purin-8- yl)piperidin-4-yl)picolinamide (56):

To an ice cold solution of tert-butyl (R)-(l-((2-((l-(6-morpholino-9H-purin-8-yl)piperidin-4- yl)carbamoyl)pyridin-4-yl)methyl)piperidin-3-yl)carbamate 55 (400 mg, 0.65 mmol) in DCM (3 mL), was added TFA (3 mL) and stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue which was neutralized with Aq. NH4OH solution, the solid so precipitated was collected by filtration and washed with water (2 x 25 mL) to get the desired product as brown solid 56 (200 mg, 59%). LCMS= [M+H] ⁺ : (520.12), Purity= 64% Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(l-(6-morpholin o-9H-purin-8- yl)piperidin-4-yl)picolinamide (Compound B0700-308):

[00658] To an ice cold stirred solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(l-(6- morpholino-9H-purin-8-yl)piperidin-4-yl)picolinamide 56 (200 mg, 0.38 mmol) in DMF (5 mL), was added EtsN (0.16 mL, 1.15 mmol) and acryloyl chloride 27 (41 pL, 0.47 mmol). The resulting reaction mass was stirred at same temperature for next 30 minutes at same temperature. After completion of reaction (TLC monitoring), the resulting reaction mass was evaporated to dryness and the crude residue was purified through RP-HPLC purification using 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column: Waters Xselect Phenyl-Hexyl(19*250mm,5pm) to get desired product as white solid Compound B0700-308 (19 mg, 9%).

¹ H NMR (400 MHz, DMSO-d6) 12.10 (s, 1H), 8.69-8.66 (d, J= 12 Hz, 1H), 8.56-8.54 (d, J= 8Hz, 1H), 8.03 (s, 1H), 8.56-8.54 (d, J= 8Hz, 1H), 7.53-7.52 (d, J = 4Hz, 1H), 6.24-6.17 (m, 1H), 6.06- 6.01 (dd, 1H), 5.59-5.53 (dd, 1H), 4.11-4.05 (m, 6H), 3.80-3.78 (br s, 1H), 3.69-3.60 (m, 7H), 3.06- 3.01 (t, 2H), 2.75-2.61 (m, 2H), 2.02-1.97(m, 1H), 1.58-1.53 (m, 3H), 1.76-1.69 (m, 5H), 1.52-1.47 (m, 1H), 1.20-1.14 (m, 1H). LCMS= [M+H] ⁺ : 575.31, Purity= 99.33%

[00659] The following compounds were or can be prepared using the appropriate reagents and the corresponding starting materials, and following the procedure described in Example B0700-4:

[00660] (S)- isomer, (S)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(l-(6-morpholin o-9H-purin-8- yl)piperidin-4-yl)picolinamide (Compound B0700-9), was synthesized similar to compound 8 in Example B0700-4 using the enantiomer of intermediate 8 in the amide bond forming step and further pursuing the sequence as described for compound 8. Example B0700-4A

Synthesis of Compound B0700-310

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(l-(7-morpholin othiazolo[5,4- d]pyrimidin-2-yl)piperidin-4-yl)picolinamide (310):

Synthesis of 4,6-dichloro-5-nitropyrimidine (95):

To an ice cold stirred solution of 5-nitropyrimidine-4,6-diol 94 (6.0 g, 38.2 mmol) in POCL (24.0 mL) was added DIPEA (33.4 mL, 191 mmol) dropwise. The reaction mixture was stirred at 100 °C for 5h. After completion of reaction (TLC monitoring), the resulting mixture was diluted with cold water (200 mL) and extracted with EtOAc (2 X 200 mL). The combined organic layer was neutralized with aqueous NaHCCL solution (200 mL), washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The resulting crude was purified by flash column chromatography (silca gel, 80 g SNAP) eluted with 15% EtOAc in heptane to get 4,6-dichloro-5- nitropyrimidine as brown solid 95 (1.8 g, 34%). 'H NMR (400 MHz, CDCL): 5 8.92 (s, 1H).

Synthesis of 4-(6-chloro-5-nitropyrimidin-4-yl)morpholine (96):

To a stirred solution of 4,6-dichloro-5-nitropyrimidine 95 (7.0 g, 36.1 mmol) and morpholine 4 (3.11 mL, 36.1 mmol) in toluene (100 mL) was added K2CO3 (4.99 g, 36.1 mmol). The resulting reaction mixture was degassed with argon for 15 min, followed by addition of rac-BINAP (1.35 g, 2.17 mmol) and Pd2(dba)3 (661 mg, 722 pmol). The resulting mixture was stirred at room temperature for 16h. After the completion of reaction (monitored by TLC), the reaction mixture was filtered through celite bed followed by washing with EtOAc (200 mL). The organic layer was concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, 80 g SNAP) eluted with 5-10% EtOAc in heptane to get desired product as yellow solid 96 (6.0 g, 68%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.53 (s, 1H), 3.67 (t, J= 4.4 Hz, 4H) and 3.53 (t, J= 4.8 Hz, 4H). LCMS: [M+H] ⁺ : 245.05, Purity= 99.94%.

Synthesis of 4-(5-nitro-6-thiocyanatopyrimidin-4-yl)morpholine (97):

To an ice cold stirred solution of 4-(6-chloro-5-nitropyrimidin-4-yl)morpholine 96 (2.70 g, 11.0 mmol) in acetic acid (20.0 mL) was added potassium thiocyanate (2.15 g, 22.1 mmol). The resulting reaction mixture was stirred at room temperature for 3h. After completion of reaction (monitored by TLC), the reaction mass was poured ice cold water (100 mL). The resulting solid precipitates was filtered, washed with diethyl ether (100 mL) and dried under reduced pressure to get desired product as yellow solid 97 (2.70 g, 92%). 'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.58 (s, 1H) and 3.68 -3.59 (m 8H). LCMS: [M+H] ⁺ : 268.01, Purity= 99.0%.

Synthesis of 7-morpholinothiazolo[5,4-d]pyrimidin-2-amine (98):

To a stirred solution of 4-(5-nitro-6-thiocyanatopyrimidin-4-yl)morpholine 97 (2.70 g, 10.1 mmol) in acetic acid (20.0 mL) was heated at 120°C followed by addition of iron powder (2.82 g, 50.5 mmol). The resulting reaction mixture was stirred at 120°C for 30 min. After completion of reaction (TLC monitoring), the reaction mixture was cooled at room temperature, filtered through sintered funnel and washed with EtOAc (2x 100 mL). The combined organic layer was concentrated under reduced pressure. The crude residue was dissolved in ethyl acetate (200 mL) and basify with aqueous NaHCOs solution (100 mL). The organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get desired compound 98 (1.90 g, 76%). LCMS: [M+H] ⁺ : 238.11, Purity= 80%.

Synthesis of 4-(2-bromothiazolo[5,4-d]pyrimidin-7-yl)morpholine (99):

To an ice cold stirred solution of 7-(morpholin-4-yl)-[l,3]thiazolo[5,4-d]pyrimidin-2-amine 98 (1.90 g, 8.01 mmol) in acetonitrile (40.0 mL) was added CuBn (2.68 g, 12.0 mmol) and tert-butyl nitrite (1.90 mL, 16.0 mmol). The resulting reaction mixture was stirred at 80°C for 16h. After completion of reaction (TLC monitoring), reaction mixture was cooled at room temperature, filtered through sintered funnel and washed with EtOAc (2 x1 00 mL). The combined organic layer was concentrated under reduced pressure and crude residue was purified by flash column chromatography in silica gel (230-400M, 40g SNAP) using 25% EtOAc in heptane to get the desired product as white solid 99 (1.90 g, 79%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.41 (s, 1H), 4.20 (br s, 4H) and 3.74 (m, 4H). LCMS: [M+H]+: (300.97), Purity= 92%.

Synthesis of tert-butyl (l-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)piperidin-4-yl )carbamate (100):

To a stirred solution of 4-{2-bromo-[l,3]thiazolo[5,4-d]pyrimidin-7-yl}morpholine 99 (900 mg, 2.99 mmol) and tert-butyl N-(piperidin-4-yl)carbamate 52 (718 mg, 3.59 mmol) in N,N- dimethylformamide (15.0 mL) was added DIPEA (2.61 mL, 14.9 mmol). The resulting reaction mixture was stirred at 80°C for 16h. After completion of reaction (TLC monitoring), reaction mixture was poured into ice cold water (100 mL). The resulting precipitates was filtered and washed with pentane (2 x 50 mL). The solid was dried under reduced pressure to get desired product 100 as white solid (1.0 g, 80%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.18 (s, 1H), 6.94-6.92 (m, 1H), 4.14 (s, 4H), 3.93-3.89 (m, 2H), 3.70-3.68 (m, 4H), 3.54 (br, s, 1H), 3.24-3.18 (m, 2H), 1.85-1.82 (m, 2H), 1.47-1.46 (m, 2H), and 1.37 (s, 9H). LCMS: [M+H]+: (421.12), Purity= 99%.

Synthesis of l-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)piperidin-4-ami ne (101):

To an ice cold solution of tert-butyl (l-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)piperidin-4- yl)carbamate 100 (250 mg, 0.83 mmol) in DCM (5 mL) was added 4N-HC1 in dioxane (2.50 mL) in drop wise. The resulting reaction mixture was stirred at room temperature for 3h. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure. The crude was triturated with toluene (10 mL) to get desired product 101 as off white solid (250 mg, quantitative, HC1 salt). ¹ HNMR (400 MHz, DMSO-t/ ₆ ): 3 8.24 (s, 1H), 8.19 (br, s, 2H), 4.10 (s, 4H), 4.05-4.02 (m, 2H), 3.71- 3.70 (m, 4H), 3.32 (br s, 1H), 3.26-3.20 (m, 2H), 2.06-2.03 (m, 2H) and 1.62-1.60 (m, 2H), LCMS: [M+H] ⁺ : 321.19, Purity= 99%.

Synthesis of tert-butyl (R)-(l-((2-((l-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)pi peridin-4- yl)carbamoyl)pyridin-4-yl)methyl)piperidin-3-yl)carbamate (102) :

To a stirred solution of 4-{[(3R)-3-{[(ter/-butoxy)carbonyl]amino}piperidin-l-yl]meth yl}pyridine-2- carboxylic acid 8 (314 mg, 936 pmol) in DMF (5 mL) was added l-[7-(morpholin-4-yl)- [l,3]thiazolo[5,4-d]pyrimidin-2-yl]piperidin-4-amine 101 (250 mg, 780 pmol), DIPEA (681 pL, 3.90 mmol) and HATU (275 mg, 1.17 mmol). The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (100 mL). The resulting precipitates was filtered and dried under reduced pressure to get as off white solid 102 (260 mg, 52%). 'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.75 (d, J = 8.0 Hz, 1H), 8.55 (d, J= 8.0 Hz, 1H), 8.19 (s, 1H), 7.97 (s, 1H), 7.52-7.51 (d, J= 4.0 Hz, 1H), 6.74-6.73 (m, 1H), 4.11 (s, 4H), 4.03-3.99 (m, 2H), 3.71- 3.69 (m, 4H), 3.58-3.56 (m, 1H), 3.41-3.32 (m, 3H), 2.73-2.71 (m, 1H), 1.90-1.88 (m, 4H), 1.82-1.72 (m, 5H), 1.50-1.45 (m, 1H), 1.38 (s, 9H), 1.23-1.11 (m, 2H) and 0.93 (m, 1H).

LCMS: [M+H] ⁺ : 638.12, Purity= 97%

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(l-(7-morpholinothia zolo[5,4- d]pyrimidin-2-yl)piperidin-4-yl)picolinamide (103):

To an ice cold solution of tert-butyl N-[(3R)-l-{[2-({ l-[7-(morpholin-4-yl)-[l,3]thiazolo[5,4- d]pyrimidin-2-yl]piperidin-4-yl}carbamoyl)pyridin-4-yl]methy l}piperidin-3-yl]carbamate 102 (260 mg, 0.40 mmol) in DCM (5 mL) was added 4N-HC1 in dioxane (2.5 mL) in drop wise. The resulting reaction mixture was stirred at room temperature for 3h. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure. The crude residue was triturated with diethyl ether get desired product 103 as pale yellow solid (240 mg, quantitative, HC1 salt). LCMS: [M+H] ⁺ : 538, Purity= 94%

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(l-(7-morpholin othiazolo[5,4- d]pyrimidin-2-yl)piperidin-4-yl)picolinamide (310):

To an ice cold stirred solution of 4-{[(3R)-3-aminopiperidin-l-yl]methyl}-N-{ l-[7-(morpholin-4-yl)- [l,3]thiazolo[5,4-d]pyrimidin-2-yl]piperidin-4-yl}pyridine-2 -carboxamide 103 (240 mg, 446 pmol) in DMF (5 mL) was added triethylamine (183 pL, 3 eq., 1.34 mmol) and prop-2-enoyl chloride 27 (40.4 mg, 1 eq., 446 pmol) in (1 mL DCM). The resulting reaction mass was stirred at same temperature for 30 minutes. After completion of reaction (TLC monitoring), poured into ice cold water (50 mL) and extracted with 20% MeOH/DCM (3 x 50 mL). The combined organic layer was washed with brine, dried over anhydrous TsfeSCU and concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel 230-400M, 12g SNAP) using 2-10% MeOH in DCM to get white solid 310 (50 mg, 19%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.75-8.73 (d, J = 8.0 Hz, 1H), 8.56 (d, J= 4.0 Hz, 1H), 8.19 (s, 1H), 7.98 (s, 1H), 7.95 (s, 1H), 7.54-7.53 (m, 1H), 6.24-6.17 (m, 1H), 6.06 (s, 1H), 5.57-5.53 (m, 1H), 4.11 (s, 5H), 4.03-3.99 (m, 2H), 3.79 (br s, 1H), 3.71-3.68 (m, 4H), 3.60 (s, 2H), 3.37-3.27 (m, 2H), 2.75-2.73 (m, 2H), 2.63-2.61 (m, 2H), 2.00-1.66 (m, 6H), 1.97 (m, 1H) and 1.10-1.07 (m, 1H). LCMS: [M+H] ⁺ : 592.33, Purity= 97%. Example B0700-5

Synthesis of Compound B0700-312

Preparation of (E)-4-(3-fluoroazetidin-l-yl)-N-(4-(2-((4-(4-morpholino-7H-p yrrolo[2,3-d]pyrimidin- 6-yl)phenyl)amino)-2-oxoethyl)phenyl)but-2-enamide (Compound B0700-312):

Synthesis of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-2 -(4- nitrophenyl)acetamide (58):

[00661] To a stirred solution of 4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)aniline 7 (450 mg, 1.523 mmol) in DMF (10 mL) was added 2-(4-nitrophenyl)acetic acid 57 (331 mg, 1.83 mmol) and EtsN (0.64 mL, 4.57 mmol) followed by the addition of BOP reagent (1.01 g, 2.28 mmol) at room temperature. The resulting reaction mixture was stirred at the same temperature for next 16h. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (3 x 50 mL), dried over Na2SO4, filtered and solvent was evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) eluted 40% ethyl acetate in heptane to get the desired product as viscous liquid 58 (300 mg, 43%).

¹ H NMR (400 MHz, DMSO-d6): 12.17 (s, 1H), 10.38 (s, 1H), 8.22-8.17 (d, J= 8.0 Hz, 2H), 8.17 (s, 1H), 8.1 (s, 1H), 7.86-7.84 (m, 4H), 7.65-7.63 (d, J= 8.0 Hz, 2H), 7.10 (s, 2H), 3.74 (m, 4H) and 3.86 (m, 4H). LCMS= [M+H] ⁺ : 459.48, Purity= 69%.

Synthesis of 2-(4-aminophenyl)-N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)acetamide (59):

To a stirred solution of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-2 -(4- nitrophenyl)acetamide 58 (300 mg, 0.87 mmol) in IPA:THF (10 mL) added Pd(OH)2 (300 mg, w/w) under nitogen atmosphere. The resulting reaction mixture was stirred under hydrogen atmosphere for 16h. After completion of reaction (TLC monitoring), the resulting reaction mixture was filtered through celite bed and solvent was evaporated under reduced pressure to get desired product as yellow solid 59 (150 mg, 53%). LCMS= [M+H] ⁺ : 429.20, Purity= 74%

Synthesis of (E)-4-(3-fluoroazetidin-l-yl)-N-(4-(2-((4-(4-morpholino-7H-p yrrolo[2,3-d]pyrimidin-6- yl)phenyl)amino)-2-oxoethyl)phenyl)but-2-enamide (Compound B0700-12):

[00662] To an ice cold stirred solution of 2-(4-aminophenyl)-N-(4-(4-morpholino-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)acetamide 59 (150 mg, 0.35 mmol) in DMF (5 mL) was added (E)-4-(3- fhioroazetidin-l-yl)but-2-enoic acid 11 (56 mg, 0.35 mmol) and DIPEA (0.31 mL, 1.75 mmol) followed by the addition of T3P (0.66 mL, 1.05 mmol). The resulting reaction mixture was stirred at room temperature for 30 min. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed brine solution (3 x 50 mL), dried over Na2SO4, filtered and evaporated under reduced pressure to get crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 2-4% MeOH in DCM to get the desired product as Off white solid Compound B0700-312 (15 mg, 8%).

¹ H NMR (400 MHz, DMSO-d6): 11.97 (s, 1H), 9.98-9.93 (m, 2H), 8.10 (s, 1H), 7.85-7.83 (d, J= 8Hz, 2H), 7.09 (s, 1H), 6.66-6.661 (m, 3H), 6.39-6.35 (m, 3H), 5.42-5.27 (m, 2H), 4.21 (m, 3H), 3.90-3.88 (m, 4H), 3.77-3.75 (m, 4H), 3.63 (s, 4H) and 3.53-3.42 (m, 2H). LCMS= [M+H] ⁺ : 570.26, Purity= 96%.

Example B0700-6

Synthesis of Compound B0700-313

[00663] Preparation of N-(4-(2-((4-(4-methyl-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl )amino)-2- oxoethyl)phenyl)acrylamide (Compound B0700-313): Synthesis of N-(4-(4-methyl-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyrro lo[2,3-d]pyrimidin- 6-yl)phenyl)-2-(4-nitrophenyl)acetamide (60):

[00664] To an ice cold stirred solution of 4-(4-methyl-7-((2-(trimethylsilyl)ethoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)aniline 24 (250 mg, 0.71 mmol) and 2-(4-nitrophenyl)acetic acid 57 (153 mg, 0.85 mmol) in DMF (3 mL) was added DIPEA (0.62 mL, 3.53 mmol) and T3P (1.35 mL, 2.12 mmol, 50% in EtOAc) in drop wise manner. The resulting reaction mixture was allowed to stir at room temperature for next 2h. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was poured into ice cold water followed by extraction with EtOAc (3 x 25 mL). The combined organic layer was washed with brine solution (3 x 20 mL), dried over Na2SO4, filtered and solvnet was evaporated under reduced pressure to get the crude which was purified by flash column chromatography over silica gel 12 g snap by using eluent 70% EtOAc in heptane to get the desired product as brown viscous liquid 60 (260 mg, 71%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 10.51 (s, 1H), 8.69 (s, 1H), 8.23 (d, J= 8.0 Hz, 2H), 7.57 (s, 2H), 7.56 (d, J= 8.0 Hz, 2H), 6.86 (s, 1H), 5.79 (s, 2H), 3.88 (s, 2H), 3.61 (t, J= 8.0 Hz, 2H), 3.17 (d, J= 5.2 Hz, 2H), 2.68 (s, 3H), 0.85 (d, J= 8.0 Hz, 2H) and 0.10 (s, 9H).

LCMS= 518.37 (M+H) ⁺ Purity: 91.23%.

Synthesis of 2-(4-aminophenyl)-N-(4-(4-methyl-7-((2-(trimethylsilyl)ethox y)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)acetamide (61):

[00665] To the stirred solution of N-[4-(4-methyl-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl]-2-(4-nitrophenyl)acetam ide 60 (260 mg, 0.50 mmol) in EtOH (9 mL) and H2O (3 mL) was added Fe powder (280 mg, 10 eq., 5.02 mmol) and NH4CI (269 mg, 5.02 mmol) at room temperature. The resulting reaction mass was stirred at 60°C for 6h. After completion of reaction (TLC monitoring), reaction mass was filtered through celite bed and solvent was evaporated under reduced pressure to get the crude residue which was diluted with water (40 mL) followed by extraction with EtOAc (3 x 50 mL). Combined organic layer was dried over Na2SO4, filtered and solvnet was evaporated under reduced pressure to get the crude as brown solid 61 (180 mg, 74%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 10.23 (s, 1H), 8.69 (s, 1H), 7.74-7.72 (m, 4H), 7.62-7.56 (m, 1H), 7.00 (d, J= 8.4 Hz, 2H), 6.86 (s, 1H), 6.53 (d, J= 7.6 Hz, 2H), 5.59 (s, 2H), 4.92 (s, 1H), 3.62 (t, J= 7.6 Hz, 2H), 3.45 (s, 2H), 1.23 (s, 2H), 0.86 (t, J= 7.2 Hz, 3H) and (s, 9H).

LCMS= [M+H] ⁺ : 488.27, Purity= 87%. Synthesis of N-(4-(2-((4-(4-methyl-7-((2-(trimethylsilyl)ethoxy)methyl)-7 H-pyrrolo [2,3- d] pyrimidin-6-yl)phenyl)amino)-2-oxoethyl)phenyl)acrylamide (62) :

[00666] To an ice cold stirred solution of 2-(4-aminophenyl)-N-(4-(4-methyl-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)acetamide 61 (175 mg, 0.36 mmol) in DMF (4 mL) was added EtsN (249 pL, 1.79 mmol) and Acryloyl chloride 27 (29.20 pL, 0.36 mmol). The resulting reaction mixture was stirred at same temperature for next 30 minutes. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was quenched with water (20 mL) followed by extraction with EtOAc (3 x 25 mL). The combined organics was washed with chilled water and brine solution (3 x 20 mL). The combined organic layer was dried over ISfeSCh, filtered and solvent was evaporated under reduced pressure to get crude which was purified by 12 g silica column eluted with 70% EtOAc in heptane to get yellow solid 62 (130 mg, 67%). LCMS= [M+H] ⁺ : 542.32, Purity= 86.15%.

Synthesis of N-(4-(2-((4-(4-methyl-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl )amino)-2- oxoethyl)phenyl)acrylamide (Compound B0700-313):

[00667] To an ice cold stirred solution of N-^-^-^-^-methyl^-^- ^rimethylsily^ethoxy^ethyl^H-pyrroloP^-dJpyrimidin^-y^phenyP amino)^- oxoethyl)phenyl)acrylamide 62 (130 mg, 0.24 mmol) in DCM (5 mL) was added TFA (5 mL) in drop wise manner. The resulting reaction mass was stirred at room temperature for next 24h. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated under reduced pressure to get the crude residue which was further basified with h Aq. NH4OH. The resulting suspension was extracted with 20% MeOH in DCM (2 x 25 mL). The combined organics was dried over Na2SO4, filtered and solvent was evaported under reduced pressure to get the crude residue, which was purified by silica column, eluted with 15% MeOH in DCM to get the desired product as off-white 313 (25 mg, 25%).

¹ H NMR (400 MHz, DMSO-t/ ₆ ): 12.40 (s, 1H), 10.30 (s, 1H), 10.12 (s, 1H), 8.57 (s, 1H), 7.90 (d, J = 8.4 Hz, 2H), 7.71 (d, J= 8.4 Hz, 2H), 7.63 (d, J= 8.4 Hz, 2H), 7.30 (d, J= 7.2 Hz, 2H), 7.02 (s, 1H), 6.45-6.39 (m, 1H), 6.25- 6.22 (m, 1H), 5.75 (d, J= 10.0 Hz, 1H), 3.62 (s, 2H) and 2.65 (s, 3H). LCMS= [M+H] ⁺ : 412.12, Purity= 98.41%. Example B0700-7

Synthesis of Compound B0700-314

[00668] Preparation of N-(6-(2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)ph enyl)amino)-

2-oxoethyl)pyridin-3-yl)acrylamide (Compound B0700-14):

Synthesis of methyl 2-(5-aminopyridin-2-yl)acetate (64):

[00669] To a stirred solution of methyl 2-(5-nitropyridin-2-yl)acetate 63 (700 mg, 3.57 mmol) in methanol (10 mL) was added Palladium on Carbon (140 mg, 1.32 mmol, 10% w/w). The resulting reaction mixture was stirred at room temperature under hydrogen atmosphere for 16h. After completion of reaction (TLC Monitoring), reaction mixture was filtered over celite pad and washed with EtOAc (100 mL). The filtrate so collected was concentrated under reduced pressure and the residue was purified by silica-gel flash column chromatography eluted with 5-50% EtOAc/Heptane to get methyl 2-(5-aminopyridin-2-yl)acetate 64 as brown solid (500 mg, 84%).

¹ H NMR (400 MHz, DMSO-t/ ₆ ): 7.82 (s, 1H), 6.97-6.95 (d, J= 8Hz, 1H), 6.88-6.87 (d, J= 4.0 Hz, 1H), 5.19 (s, 2H) and 3.60 (s, 3H). LCMS= [M+H] ⁺ : 167.18, Purity= 94%.

Synthesis of methyl 2-(5-acrylamidopyridin-2-yl)acetate (65):

[00670] To an ice cold stirred solution of methyl 2-(5-aminopyri din-2 -yl)acetate (220 mg, 1.32 mmol) 64 in DCM (5.0 mL), was added EtsN (0.54 mL, 3.97 mmol) and acryloyl chloride 27 (0.235 mL, 2.65 mmol) in drop wise manner. The resulting reaction mass was stirred at same temperature for next 15 minutes. After completion of reaction (TLC monitoring), solvent was evaporated up to dryness to get crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) eluted 2-4% MeOH in DCM to get the desired product as yellow solid 65 (210 mg, 72%). 'H NMR (400 MHz, DMSO-t/ ₆ : 3 10.34 (s, 1H), 8.69 (s, 1H), 8.07-8.05 (d, J= 8.0 Hz, 1H), 7.33- 7.31(d, J = 8.0 Hz, 1H), 6.46-6.40 (m, 1H), 6.30-6.26 (d, J= 16 Hz, 1H), 5.81-5.78 (d, J= 9.20 Hz, 1H), 3.80 (s, 2H), 3.61 (s, 3H) and 2.60 (s, 1H). LCMS= [M+H] ⁺ : 221.08, Purity= 84%. Synthesis of 2-(5-acrylamidopyridin-2-yl)acetic acid (66):

To a stirred solution of methyl 2-[5-(prop-2-enamido)pyridin-2-yl]acetate 65 (210 mg, 954 pmol) in 1,2-di chloroethane (1.00 mL) then a trimethyl stannanol (349 mg, 1.91 mmol) was added at 0 °C. The resultant reaction mixture was stirred at 80 °C for 16 h. After completion of reaction (TLC Monitoring), all volatile were removed under reduced pressure and the residues was taken in water (10 mL) and acidified with cone, acetic acid up to pH 5 then extracted with EtOAc (5 x 20 mL). Combined organic layers dried over anhydrous Na2SO4, concentrated under reduced pressure to get crude product 2-[5-(prop-2-enamido)pyridin-2-yl]acetic acid 66 as yellow solid (150 mg, Yield: 76 %). LCMS= [M+H] ⁺ : 207.07, Purity= 64%.

Synthesis of N-(6-(2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)ph enyl)amino)-2- oxoethyl)pyridin-3-yl)acrylamide (Compound B0700-314):

[00671] To a stirred solution of 2-(5-acrylamidopyridin-2-yl)acetic acid 66 (150 mg, 0.73 mmol) in DMF (10 mL) was added 4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)aniline 7 (215 mg, 0.727 mmol) DIPEA (0.381 mL, 2.18 mmol) and HATU (257 mg, 1.09 mmol) at room temperature. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed brine solution (3 x 50 mL), dried over ISfeSCL, filtered and evaporated under reduced pressure to get the crude residue. The crude residue was purified through RP-HPLC purification using 5mM Ammonium bicarbonate in water/ Acetonitrile (column: Waters Xselect Phenyl-Hexyl(19*250mm,5pm) to get desired product as white solid 314 (15 mg, 4%).

¹ H NMR (400 MHz, DMSO-d6): 5 12.15 (s, 1H), 10.34 (s, 1H), 8.75 (s, 1H), 8.17 (s, 1H), 8.05-8.03 (m, 1H), 7.83-7.81 (d, J= 8.0 Hz, 2H), 7.67-7.65 (d, J = 8.0 Hz, 2H), 7.39-7.36 (d, J = 12.0 Hz, 1H), 7.00 (s, 1H), 6.43-6.41 (m, 1H), 6.31 (dd, 1H), 5.79-5.76 (dd, 1H), 3.90-3.38 (m, 4H), 3.83 (s, 2H), 3.77-3.75 (m, 4H). LCMS= [M+H] ⁺ : 484.20, Purity= 96.40%.

Example B0700-8

Synthesis of Compound B0700-315

[00672] Preparation of N-(4-(2-((l-(6-morpholino-9H-purin-8-yl)piperidin-4-yl)amino )-2- oxoethyl)phenyl)acrylamide (Compound B0700-315):

Synthesis of N-(l-(6-morpholino-9H-purin-8-yl)piperidin-4-yl)-2-(4-nitrop henyl)acetamide (67):

To an ice cold stirred solution of N-(l-(6-morpholino-9H-purin-8-yl)piperidin-4-yl)-2-(4- nitrophenyl)acetamide 67 (300 mg, 0.990 mmol) in DMF (10 mL) were added 2-(4- nitrophenyl)acetic acid 57 (215 mg, 1.188 mmol), DIPEA (0.879 mL, 4.95 mmol) and T3P (50% in ethyl acetate ; 1.88 mL, 2.97 mmol) and stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated to dryness and purified by flash column chromatography (silica gel, 12 g SNAP) eluted with 2-3% MeOH in DCM to get the desired product as Off white solid 67 (200 mg). LCMS= [M+H] ⁺ : 467.21, Purity= 44%.

Synthesis of 2-(4-aminophenyl)-N-(l-(6-morpholino-9H-purin-8-yl)piperidin -4-yl)acetamide (68): [00673] To a stirred solution of N-(l-(6-morpholino-9H-purin-8-yl)piperidin-4-yl)-2-(4- nitrophenyl)acetamide 67 (200 mg, 0.43 mmol) in EtOH and water (12 mL, 3: 1 ratio) was added Fe powder (119 mg, 2.143 mmol), NH4CI (114 mg, 2.143 mmol). The resulting reaction mixture was stirred at 70°C for 2h. After completion of reaction (TLC monitoring), the reaction mixture was filtered over celite bed and washed with ethanol (2 x 10 mL). Solvent was evaporated under reduced pressure to get crude 68 as brown solid (174 mg, 90%).

¹ H NMR (400 MHz, DMSO-d6)): 5 12.06 (s, 1H), 8.03 (s, 1H), 7.85-7.83 (d, J = 8.40 Hz, 1H), 6.89- 6.87 (d, J= 8Hz, 2H), 6.48-6.46 (d, J = 8Hz, 2H), 5.83 (s, 2H), 4.85 (s, 2H), 4.0 (m, 2H), 3.98-3.95 (m, 5H), 3.75 (m, 4H), 3.06 (m, 2H), 1.78-1.66 (m, 2H), 1.45-1.35 (m, 2H).

LCMS= [M+H] ⁺ : 437.23, Purity= 74%. Synthesis of N-(4-(2-((l-(6-morpholino-9H-purin-8-yl)piperidin-4-yl)amino )-2- oxoethyl)phenyl)acrylamide (Compound B0700-315):

[00674] To an ice cold stirred solution of 2-(4-aminophenyl)-N-(l-(6-morpholino-9H-purin-8- yl)piperidin-4-yl)acetamide 68 (174 mg, 0.399 mmol) in DMF (5.0 mL) was added EtsN (0.162 mL, 1.197 mmol) and acryloyl chloride 27 (43 pL, 0.478 mmol) in drop wise manner. The resulting reaction mixture was stirred at same temperature for next 30 minutes. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated up to dryness and the crude residue was purified through RP-HPLC purification eluted with 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column: Waters Xselect Phenyl-Hexyl(19*250mm,5pm) get desired product as white solid 315 (15 mg, 7%).

¹ H NMR (400 MHz, DMSO-d6): 5 11.79 (s, 1H), 9.83 (s, 1H), 8.03 (s, 1H), 7.74-7.72 (d, J= 8.0 Hz, 1H), 7.57-7.55 (d, J= 8.0 Hz, 1H), 7.21-7.19 (d, J= 8.0 Hz, 2H), 6.46-6.39 (m, 1H), 6.26-6.21 (dd, 1H), 5.72-5.69 (dd, 1H), 4.00 (m, 4H), 3.96 (m, 2H), 3.82-3.80 (m, 1H), 3.70-3.68 (m, 4H), 3.36 (s, 2H), 3.13-3.00 (m, 3H), 1.84-1.80 (m, 2H), 1.51-1.42 (m, 2H)

LCMS= [M+H] ⁺ : 491.24, Purity= 97.77%.

Example B0700-9

Synthesis of Compound B0700-316

Preparation of N-(6-(2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)ph enyl)amino)-2- oxoethyl)phenyl)acrylamide (Compound B0700-316):

Synthesis of methyl 2-(4-aminopyridin-2-yl)acetate (64a):

[00675] To a stirred solution of methyl 2-(4-nitrophenyl)acetate 63a (700 mg) in methanol (10 mL) is added Palladium on Carbon (140 mg, 1.32 mmol, 10% w/w). The resulting reaction mixture is stirred at room temperature under hydrogen atmosphere for 16h. After completion of reaction (TLC Monitoring), reaction mixture is filtered over celite pad and washed with EtOAc (100 mL). The filtrate so collected is concentrated under reduced pressure and the residue is purified by silica-gel flash column chromatography eluted with 5-50% EtOAc/Heptane to get methyl 2-(4- aminophenyl)acetate 64a as solid.

Synthesis of methyl 2-(4-acrylamidophenyl)acetate (65a):

[00676] To an ice cold stirred solution of methyl 2-(4-aminophenyl)acetate (220 mg) 64a in DCM (5.0 mL), is added EtsN (0.54 mL, 3.97 mmol) and acryloyl chloride 27 (0.235 mL, 2.65 mmol) in drop wise manner. The resulting reaction mass is stirred at same temperature for next 15 minutes. After completion of reaction (TLC monitoring), solvent is evaporated up to dryness to get crude residue, which is purified by flash column chromatography (silica gel, 40 g SNAP) eluted 2-4% MeOH in DCM to get the desired product as yellow solid 65a.

Synthesis of 2-(4-acrylamidophenyl)acetic acid (66a):

[00677] To a stirred solution of methyl 2-[4-(prop-2-enamido)phenyl]acetate 65a (210 mg) in 1,2- di chloroethane (1.00 mL) then a trimethyl stannanol (349 mg, 1.91 mmol) is added at 0 °C. The resultant reaction mixture is stirred at 80 °C for 16 h. After completion of reaction (TLC Monitoring), all volatile were removed under reduced pressure and the residues is taken in water (10 mL) and acidified with cone, acetic acid up to pH 5 then extracted with EtOAc (5 x 20 mL). Combined organic layers dried over anhydrous Na2SO4, concentrated under reduced pressure to get crude product 2-[5-(prop-2-enamido)pyridin-2-yl]acetic acid 66a as yellow solid.

Synthesis of N-(6-(2-((4-(4-morpholino(t/8)-7H-pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)amino)-2- oxoethyl)phenyl)acrylamide (Compound B0700-316):

[00678] To a stirred solution of 2-(4-acrylamidophenyl)acetic acid 66a (150 mg, 0.73 mmol) in DMF (10 mL) is added 4-(4-morpholino(d8)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)aniline 7 (215 mg) DIPEA (0.381 mL, 2.18 mmol) and HATU (257 mg, 1.09 mmol) at room temperature. The resulting reaction mixture is stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting reaction mass is poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer is ished brine solution (3 x 50 mL), dried over Na2SO4, filtered and evaporated under reduced pressure to get the crude residue. The crude residue is purified through RP-HPLC purification using 5mM Ammonium bicarbonate in water/ Acetonitrile (column: Waters Xselect Phenyl-Hexyl(19*250mm,5pm) to get desired product as white solid 316. Example B0700-9A

Synthesis of Compound B0700-317

Preparation of N-(l-(2-(3-(l-(6-morpholino-9H-purin-8-yl)piperidin-4-yl)ure ido)pyridin-4- yl)azetidin-3-yl)acrylamide (317):

A solution of l-[4-(3-aminoazetidin-l-yl)pyridin-2-yl]-3-{ l-[6-(morpholin-4-yl)-9H-purin-8- yl]piperidin-4-yl}urea 121 (140 mg, 284 pmol) in THF: water (2: 1, 15.0 mL) was heated at 55°C for 20 min (clear solution observed), followed by addition of K3PO4 (120 mg, 567 pmol) portion wise and stirred the reaction mass at same temperature for 30 min (clear solution observed). The resulting reaction mixture was cooled to 10°C and added 3 -chloropropionyl chloride 122 (0.05 mL, 340 pmol) in drop-wise. The resulting reaction mixture was stirred at rt for 2h. After completion of reaction (TLC monitoring), cooled the reaction mass and added 2M solution of NaOH dropwise. The resulting reaction mass was stirred for 16h at room temperature. After completion of reaction (progress of reaction monitored by LC-MS), the resulting reaction mass was diluted with water (50 mL) and extracted with EtOAc (2 x 100 mL). The combined organic layer was concentrated under reduced pressure. The crude residue was purified by RP-HPLC purification using 5mM Ammonium Bicarbonate in Water/ Acetonitrile and column Waters XSelect Phenyl-Hexyl(19*250mm,5pm) to get desired product as white solid 317 (18 mg, 10%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 8 11.93 (s, 1H), 8.80 (s, 1H), 8.68 (br s, 1H), 8.01 (s, 1H), 7.78 (d, J = 6.0 Hz, 1H), 7.43 (d, J= 7.6 Hz, 1H), 6.84-6.78 (m, 1H), 6.22 (br s, 1H), 6.11-6.04 (m, 2H), 5.67- 5.64 (m, 1H), 4.63-4.61 (m, 1H), 4.19-4.12 (m, 3H), 4.00-3.91 (m, 9H), 3.78 (m, 1H), 3.62-3.60 (m, 2H), 3.25 (m, 1H), 3.00-2.94 (m, 1H), 1.90-1.86 (m, 2H) and 1.31-1.23 (m, 2H).

LCMS: [M+H] ⁺ : 548.08, Purity= 97%. Example B0700-9B

Synthesis of Compound B0700-320

Preparation of (E)-4-(azetidin-l-yl)-N-(l-(2-(3-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin- 6-yl)phenyl)ureido)pyridin-4-yl)azetidin-3-yl)but-2-enamide (320):

Synthesis of tert-butyl (l-(2-nitropyridin-4-yl)azetidin-3-yl)carbamate (106):

[00679] To a stirred solution of 4-chloro-2-nitropyridine 104 (3.0 g, 18.9 mmol) in 1,4-dioxane (10 mL) was added DIPEA (19.8 mL, 114 mmol) and tert-butyl N-(azetidin-3-yl)carbamate 105 (4.89 g, 28.4 mmol). The resulting reaction mixture was stirred at 140°C in microwave for 2h. After completion of the reaction (TLC monitoring), the reaction mixture was diluted with water (100 mL) and extracted with EtOAc (2 x 100 mL ). The combined organic layer was dried over anhydrous Na2SO4, filtered and solvent was evaporated under reduced pressure. The crude was purified by flash column chromatoghaphy using silica gel (230-400M, 40g SNAP) eluted with 10-20% EtOAc in hexane to get the desired product as yellow solid 106 (3.0 g, 54%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.12 (d, J = 5.6 Hz, 1H), 7.66 (d, J= 6.8 Hz, 1H), 7.12 (s, 1H), 6.70-6.69 (m, 1H), 4.48-4.46 (m, 1H), 4.30-4.26 (m, 2H), 3.87-3.85 (m, 2H) and 1.39 (s, 9H).

LCMS = [M+H] ⁺ : 295.14, Purity= 97%.

Synthesis of tert-butyl (l-(2-aminopyridin-4-yl)azetidin-3-yl)carbamate (107):

[00680] To a stirred solution of tert-butyl N-[l-(2-nitropyridin-4-yl)azetidin-3-yl]carbamate 106 (2.20 g, 7.48 mmol) in IPA:THF (10 mL, 2: 1) was added Pd/C (0.22 g, 10 % w/w) under nitogen atmosphere. The resulting reaction mixture was stirred under hydrogen atmosphere for 4h. After completion of reaction (TLC monitoring), the resulting reaction mixture was filtered through celite bed and solvent was evaporated under reduced pressure to get desired product as pale yellow solid 107 (1.80 g, 91%). LCMS: [M+H] ⁺ : 265.13, Purity= 97%

Synthesis of tert-butyl (l-(2-(3-(4-bromophenyl)ureido)pyridin-4-yl)azetidin-3-yl)ca rbamate (109):

[00681] To the stirred solution of tert-butyl N-[l-(2-aminopyridin-4-yl)azetidin-3-yl]carbamate 107 (2.50 g, 9.46 mmol) in toluene (10 mL) was added l-bromo-4-isocyanatobenzene 108 (1.87 g, 9.46 mmol). The resulting reaction mixture was stirred at 110°C for 16h. After completion of the reaction (TLC monitoring ), the reaction mixture was cooled and poured into ice water (50 mL). The solid precipitates was filtered and washed with diethyl ether (2 x 50mL). The solid was dried under reduced pressure to get desired product as white solid 109 (2.0 g, 46%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 11.36 (s, 1H), 9.22 (s, 1H), 7.87-7.86 (m, 1H), 7.58 (br s, 1H), 7.58-7.55 (m, 4H), 6.26-6.23 (m, 1H), 6.08-6.07 (m, 1H), 4.43 (m, 1H), 4.14-4.10 (m, 2H), 3.70-3.67 (m, 2H) and 1.38 (s, 9H).

Synthesis of tert-butyl (l-(2-(3-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)phenyl)ureido)pyridin-4-yl)azetidin-3-yl)carbamate (111):

[00682] To a stirred solution of tert-butyl N-[l-(2-{[(4-bromophenyl)carbamoyl]amino}pyridin-4- yl)azeti din-3 -yl] carbamate 109 (2.0 g, 4.33 mmol) in DMSO (20 mL) was added 4, 4, 5, 5- tetramethyl-2-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)- l,3,2-dioxaborolane 110 (1.65 g, 6.49 mmol) and the reaction mixture was degassed with nitrogen for 15 min, followed by addition of potassium acetate (1.27 g, 13.0 mmol). The resulting reaction mixture was degassed again with nitrogen for additional 10 min, then Pd(dppf)C12.DCM complex (353 mg, 0.43 mmol) was added and resulting reaction mass was stirred at 100°C for 16h. After the completion of the reaction (TLC monitoring), reaction mixture was cooled at room temperature, diluted with ice cold water (50 mL ) and extracted with EtOAc (2 X 100 mL). The combined organic layer was washed with brine, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure to get crude residue 111 (2.0 g, 48%). The crude was used for further next step without purification.

Synthesis of tert-butyl (l-(2-(3-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methy l)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)ureido)pyridin-4-yl)azet idin-3-yl)carbamate (112):

[00683] To a stirred solution of terLbutyl N-{ l-[2-({[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)phenyl]carbamoyl}amino)pyridin-4-yl]azetidin-3-yl}carbama te 111 (800 mg, 1.57 mmol) in 1,4- dioxane (18.0 mL) was added 4-(6-iodo-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2, 3- d]pyrimidin-4-yl)morpholine 5 (1.08 g, 2.36 mmol). The reaction mixture was degassed with nitrogen for 15 min, followed by addition of aqueous solution sodium carbonate (499 mg, 4.71 mmol) in water (2.0 mL) and degassed again with nitrogen for additional 10 min, then PdC12(dppf).DCM complex (128 mg, 157 pmol) was added. The reaction mixture was stirred at 100°C for 16h. After the completion of the reaction (monitored by TLC), reaction mixture was cooled at room temperature and diluted with ice cold water (50 mL ) and extracted with EtOAc (2 x 50 mL). The combined organic layer was washed with brine), dried over anhydrous Na2SO4 and evapourated under reduced pressure. The crude was purified by silica-gel combi-flash column chromatography eluted with 9% EtOAc/heptane to get desired product as a yellow solid 112 (400 mg, 35.58%).

'H NMR (400 MHz, DMSO-t/ ₆ : 3 11.11 (s, 1H), 9.19 (s, 1H), 8.26 (s, 1H), 7.90.7.89 (m, 1H), 7.70- 7.68 (m, 2H), 7.64-7.62 (m, 2H), 7.57-7.55 (m, 1H), 7.48-7.46 (m, 1H), 6.88 (s, 1H), 6.29 (s, 1H), 6.10-6.08 (m, 1H), 5.54 (s, 2H), 4.43 (m, 1H), 4.16-4.14 (m, 2H), 3.88 (m, 4H), 3.73-3.72 (m, 6H), 3.63-3.61 (m, 2H), 1.39 (s, 8H), 0.88-0.84 (m, 2H) and -0.04- -0.07 (s, 9H) LCMS: [M+H] ⁺ : (716.18), Purity= 89%

Synthesis of l-(4-(3-aminoazetidin-l-yl)pyridin-2-yl)-3-(4-(4-morpholino- 7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)urea (113):

[00684] An ice cold stirred solution of tert-butyl N-(l-{2-[({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)amino]pyridin- 4-yl}azetidin-3-yl)carbamate 112 (300 mg, 419 pmol) in 25% TFA in DCM (10 mL) was stirred for 2h. After completion of the reaction (monitored by TLC), the reaction mixture was concentrated under reduced pressure to get crude and used as such for further next step without purification.

Synthesis of (E)-4-(azetidin-l-yl)-N-(l-(2-(3-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)ureido)pyridin-4-yl)azetidin-3-yl)but-2-enamide (320):

[00685] To an ice cold stirred solution of l-[4-(3-aminoazetidin-l-yl)pyridin-2-yl]-3-{4-[4- (morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}urea 113 (170 mg, 350 pmol) in DMF (4.0 mL) was added DIPEA (136 mg, 1.05 mmol), (2E)-4-(azetidin-l-yl)but-2-enoic acid 30 (49.4 mg, 350 pmol) and T3P (50% in ethyl acetate, 0.15 mL, 1.05 mmol). The resulting reaction mixture was stirred at room temperature for Ih. After completion of the reaction (monitored by TLC), the reaction mixture was diluted with ice cold water (50 mL) and extracted with 10% MeOH in DCM (3 x 50 mL). The combined organic layer was dried over anhydrous ISfeSCU, filtered and evaporated under reduced pressure. The crude was purified by RP-HPLC eluted with 5mM ammonium bicarbonate in water/acetonitrile in waters XSelect CSH C-18(19*250mm,10pm) flow rate acetonitrile(%)-35-70%, Flow-18. Oml/min, 16 min to get as white solid 320 (30 mg, 14%). 'H NMR (400 MHz, DMSO-t/ ₆ : 5 12.15 (s, 1H), 11.84 (s, 1H), 9.21 (s, 1H), 8.68-8.66 (m, 1H), 8.17 (s, 1H), 7.92-7.91 (m, 1H), 7.84 (m, J = 8.0 Hz, 2H), 7.58 (d, J = 8.0 Hz, 2H), 7.07 (s, 1H), 6.54-6.41 (m, 1H), 6.31-6.29 (m, 1H), 6.13-6.11 (m, 1H), 6.00-5.96 (m, 1H), 4.70-4.63 (m, 1H), 4.22-4.18 (m, 2H), 3.89-3.86 (m, 4H), 3.76-3.74 (m, 4H), 3.13-3.08 (m, 3H), 2.00-1.93 (m, 2H), 1.92-1.88 (m, 1H), 1.77-1.68 (m, 2H), 1.55-1.52 (m, 1H) and 1.25-1.14 (m, 1H). LCMS: [M+H] ⁺ : (609.16), Purity= 98.36%

Example B0700-9C

Synthesis of Compound B0700-321

Preparation of (E)-4-(azetidin-l-yl)-N-(l-(2-(3-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin- 6-yl)phenyl)ureido)pyridin-4-yl)azetidin-3-yl)but-2-enamide (321):

Synthesis of benzyl 4-(chlorocarbonyl)piperidine-l-carboxylate (115):

[00686] To an ice cold stirred solution of l-[(benzyloxy)carbonyl]piperidine-4-carboxylic acid 114 (3.0 g, 11.4 mmol) in dichloromethane (30 mL) was added oxalylchloride (1.95 mL, 22.8 mmol) and N,N-dimethylformamide (cat.). The resulting reaction mixture was stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure (under nitrogen atmosphere) to get crude product 115 (3.0 g, colourless gel). The crude was used as such for next step.

Synthesis of benzyl 4-(azidocarbonyl)piperidine-l-carboxylate (116):

[00687] To an ice-cold stirred solution of benzyl 4-(chlorocarbonyl)piperidine-l -carboxylate 115 (3.0 g, 10.4 mmol) in acetone (30 mL) was addded a solution of NaNs (1.48 g, 22.8 mmol) in water (30 mL) dropwise. The resultant reaction mixture was stirred at 0°C for 2h. After completion of reaction (TLC monitoring), organic part was concentrated under reduced pressure and aqueous part was extracted with DCM (3 x 100 mL). The combined organic layer was washed with brine (50 mL), dried over anhydrous Na2SO4 and concentrated under reduced pressure to get desired product as a viscous liquid 116 (3.0 g, 91% after two steps). The cude was used directly for the next step. Synthesis of benzyl 4-(3-(4-(3-((tert-butoxycarbonyl)amino)azetidin-l-yl)pyridin -2- yl)ureido)piperidine-l-carboxylate (118):

[00688] A solution of benzyl 4-(azidocarbonyl)piperidine-l -carboxylate 116 (1.80 g, 6.24 mmol) in toluene (30.0 mL) was heated at 90°C for 2h. After completion of starting material, (TLC monitoring), reaction mixture was cooled to 0°C and tert-butyl N-[l-(2-aminopyridin-4-yl)azetidin- 3-yl]carbamate 107 (1.65 g, 6.24 mmol) was added. The resultant reaction mixture was heated at 120°C for 16h. After completion of reaction (monitored by TLC), reaction mixture was cooled to room temperature, diluted with water (100 mL) and extracted with 20% IP A: Chloroform (3 x 100 mL). The combined organic layer washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified by silica-gel flash column chromatography (silica 230-400M) eluted with 2-5% MeOH:DCM to get the desired product as white solid 118 (1.30 g, 40% after two steps).

LCMS: [M+H] ⁺ : 525.31, Purity= 75%.

Synthesis of tert-butyl (l-(2-(3-(piperidin-4-yl)ureido)pyridin-4-yl)azetidin-3-yl)c arbamate (119):

[00689] To a stirred solution of benzyl 4-({[4-(3-{[(tert-butoxy)carbonyl]amino}azetidin-l- yl)pyridin-2-yl]carbamoyl}amino)piperidine-l-carboxylate 118 (1.30 g, 2.48 mmol) in IPA:THF (40 mL) was added Pd(OH)2 (650 mg, 50% w/w) under nitogen atmosphere. The resulting reaction mixture was stirred under hydrogen atmosphere for 16h. After completion of reaction (TLC monitoring), the resulting reaction mixture was filtered through celite bed and solvent was evaporated under reduced pressure to get desired product 119 as white solid (800 mg, 83%).

LCMS: [M+H] ⁺ : 391.23, Purity= 93%

Synthesis of tert-butyl (l-(2-(3-(l-(6-morpholino-9-((2-(trimethylsilyl)ethoxy)methy l)-9H-purin- 8-yl)piperidin-4-yl)ureido)pyridin-4-yl)azetidin-3-yl)carbam ate (120):

[00690] To a stirred solution of tert-butyl N-[l-(2-{[(piperidin-4-yl)carbamoyl]amino}pyridin-4- yl)azeti din-3 -yl] carbamate 119 (300 mg, 768 pmol) in 1,4-dioxane (20 mL) was added CS2CO3 (751 mg, 2.30 mmol) and 8-bromo-6-(morpholin-4-yl)-9-{[2-(trimethylsilyl)ethoxy]meth yl}-9H-purine 51 (318 mg, 768 pmol). The resulting reaction mixture was degassed with nitrogen for 15 minutes, followed by addition of Pd2(dba)3 (70.4 mg, 76.8 pmol) and Xantphos (88.9 mg, 154 pmol). The resulting reaction mixture was heated at 100°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed and washed with ethyl acetate (3 x 50 mL). The combined organic layer was washed with water and brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 120 g SNAP) eluted with 2-3% MeOH in DCM to get desired product as yellow solid 120 (200 mg, 36%).

LCMS: [M+H] ⁺ : 724.36, Purity= 89%.

Synthesis of l-(4-(3-aminoazetidin-l-yl)pyridin-2-yl)-3-(l-(6-morpholino- 9H-purin-8- yl)piperidin-4-yl)urea (121):

[00691] To an ice cold solution of tert-butyl N-(l-{2-[({ l-[6-(morpholin-4-yl)-9-{[2- (trimethylsilyl)ethoxy]methyl}-9H-purin-8-yl]piperidin-4-yl} carbamoyl)amino]pyri din-4- yljazeti din-3 -yl)carbamate 120 (280 mg, 387 pmol) in DCM (5.00 mL) was added TFA (4.00 mL) and the reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude was neutralized with aqueous solution of NH4OH, the solid precipitates was filtered and drided under vaccum to get the desired product as brown solid 121 (200 mg, quantitative).

LCMS: [M+H] ⁺ : (494.04), Purity= 65%.

Synthesis of (E)-4-(azetidin-l-yl)-N-(l-(2-(3-(l-(6-morpholino-9H-purin-8 -yl)piperidin-4- yl)ureido)pyridin-4-yl)azetidin-3-yl)but-2-enamide (321):

[00692] To an ice cold stirred solution of (2E)-4-(azetidin-l-yl)but-2-enoic acid 30 (57.2 mg, 405 pmol) in N,N-dimethylformamide (1.00 mL) was added DIPEA (34.1 pL, 195 pmol) and T3P (50% solution in ethyl acetate, 0.4 mL, 1.22 mmol). The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure. The crude residue was purified by RP-HPLC purification using 0.1% ammonium hydroxide in water/acetonitrile and column Waters XSelect CSH C-18(19*250mm,10pm) to get the desired product as white solid 321 (25 mg, 10%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8.52 (s, 1H), 7.99 (s, 1H), 7.79 (d, J= 4.0 Hz, 1H), 7.01-7.00 (m, 1H), 6.46 (s, 2H), 6.29-6.28 (m, 1H), 6.03-6.01 (m, 1H), 4.67 (m, 1H), 4.22-4.11 (m, 3H), 4.00- 3.89 (m, 7H), 3.80 (m, 1H), 3.64-3.62 (m, 4H), 3.16-3.11 (m, 8H), 2.03-1.96 (m, 4H), 1.89-1.86 (m, 2H) and 1.38-1.31 (m, 2H). LCMS: [M+H] ⁺ : 617.13, Purity= 97%. Example B0700-9D

Synthesis of Compound B0700-323

Preparation of of N-(l-(2-(2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)amino)-2-oxoethyl)pyrimidin-4-yl)azetidin-3-yl)acr ylamide (323):

Synthesis of tert-butyl (l-(2-methylpyrimidin-4-yl)azetidin-3-yl)carbamate (129):

[00693] To a stirred solution of 4-chloro-2-methylpyrimidine 128 (3.00 g, 23.3 mmol) in 1,4- dioxane (10 mL) was added DIPEA (24.5 mL, 140 mmol) followed by addtion of tert-butyl N- (azeti din-3 -yl)carbamate 105 (6.03 g, 35.0 mmol) and resulting reaction mixture was heated at 140°C for 2h. After completion of the reaction (monitored by TLC ), the reaction mixture was diluted with water (100 mL) and extracted with EtOAc (2 x 100 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude was purified by combi-flash column chromatography (silica gel, 40 g SNAP) eluted with 5% MeOH in DCM to get the desired product as white solid 129 (3.0 g, 47%).

'H NMR (400 MHz, DMSO-t/ ₆ : 8 8.05 (d, J= 4.0 Hz, 1H), 7.60-7.58 (m, 1H), 6.19 (d, J = 8.0 Hz, 1H), 4.43-4.41 (m, 1H), 4.22-4.17 (m, 2H), 3.81-3.79 (m, 2H), 2.33 (s, 3H), and 1.38 (s, 9H) LCMS: [M+H] ⁺ : 265.17, Purity= 97%

Synthesis of 2-(4-(3-((tertebutoxycarbonyl)amino)azetidin-l-yl)pyrimidin- 2-yl)acetic acid (130): [00694] To a solution of tert-butyl N-[l-(2-methylpyrimidin-4-yl)azeti din-3 -yl]carbamate 129 (2.0 g, 7.57 mmol) in THF (50 mL) was cooled to -78°C, followed by addition of LDA (22.7 mL, 45.4 mmol) and resulting reaction mixture stirred at same temperature for 45 min, the reaction mass was purged with CO2 gas at -78°C for 20 min. The resulting reaction mixture was stirred at room temperature for 30 min. After completion of the reaction (TLC monitoring ), the reaction mixture was diluted with water (50 mL) and extracted with EtOAc (2 x 100 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and concentrated under reduced pressure to get desired product as white solid 130 (2.0 g, 68%). flT NMR (400 MHz, DMSO-t/ ₆ ): 5 8.01-8.00 (m, 1H), 7.65-7.64 (m, 1H), 6.15 (s, 1H), 4.41 (m, 1H), 4.21-4.19 (m, 2H), 3.79 (m, 2H), 3.23 (s, 2H) and 1.38 (s, 9H). LCMS: [M+H] ⁺ : 309.10, Purity= 80% Synthesis of tert-butyl N-(l-{2-[({4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethox y]methyl}- 7H-pyrrolo [2, 3-d] pyrimidin-6-yl] phenyl} carbamoyl)methyl] pyrimidin-4-yl} azetidin-3- yl)carbamate (131):

[00695] To an ice cold stirred solution of 4-[4-(morpholin-4-yl)-7-{[2-

(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin -6-yl]aniline 7 (1.00 g, 2.35 mmol) in DMF (10 mL) was added 2-[4-(3-{[(tert-butoxy)carbonyl]amino}azetidin-l-yl)pyrimidi n-2-yl]acetic acid 130 (941 mg, 3.05 mmol) and DIPEA (2.43 mL, 14.09 mmol) followed by the addition of T3P (50% in EtOAc, 7.47 mL, 11.75 mmol). The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed with brine solution, dried over ISfeSCU, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 10-20% EtOAc in heptane to get the desired product as brown semi-solid 131 (500 mg, 28%). LCMS: [M+H] ⁺ : 716.48, Purity= 97%.

Synthesis of 2-(4-(3-aminoazetidin-l-yl)pyrimidin-2-yl)-N-(4-(4-morpholin o-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)acetamide (132):

[00696] To an ice cold solution of tert-butyl N-(l-{2-[({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl} carbarn oyl)methyl]pyrimidin-4-yl}azeti din-3 -yl)carbamate 131 (500 mg, 0.69 mmol) in DCM (10 mL) was added TFA (5.0 mL) in drop wise. The resulting reaction mixture was stirred at room temperature for 6h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude was triturated with diethyl ether (50 mL) to get the desired product 132 as off white solid (330 mg, 74%). LCMS: [M+H] ⁺ : 486.26, Purity= 76%.

Synthesis of N-(l-(2-(2-((4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)amino)-2- oxoethyl)pyrimidin-4-yl)azetidin-3-yl)acrylamide (323) :

[00697] To an ice cold stirred solution of 2-[4-(3-aminoazetidin-l-yl)pyrimidin-2-yl]-N-{4-[4- (morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}acet amide 132 (228 mg, 0.47 mmol) in DMF (5.0 mL) was added EtsN (0.20 mL, 1.41 mmol) and acryloyl chloride 27 (42.5 pL, 470 pmol) in drop wise. The resulting reaction mixture was stirred at same temperature for 30 minutes. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure. The crude was purified through flash column chromatography (silica gel, 12g SNAP) using eluents 2-3% MEOH in DCM to get desired product as white solid 323 (25 mg, 10%).

¹ H NMR (400 MHz, DMSO-t/ ₆ : 8 12.15 (s, IH), 10.28 (s, IH), 8.82 (d, J = 7.2 Hz, IH), 8.17-8.14 (m, 2H), 7.85-7.83 (m, 2H), 7.67-7.64 (m, 2H), 7.09 (s, IH), 6.33-6.31 (m, IH), 6.23-6.09 (m, 2H), 5.66- 5.63 (m, IH), 4.72-4.68 (m, IH), 4.33-4.29 (m, 2H), 3.88-3.86 (m, 6H) and 3.75-3.72 (m, 6H). LCMS: [M+H] ⁺ : 540.29, Purity= 96%.

Example B0700-10

Synthesis of Compound B0700-330

[00698] Preparation of l-(l-(l-acryloylazetidin-3-yl)piperidin-4-yl)-3-(4-(4-morpho lino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)urea (Compound B0700-330):

Synthesis of tert-butyl 3-(4-(3-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)ureido)piperidin-l-yl)azetidine-l-carboxylate (77):

|00699] To an ice cold stirred solution of l-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-3-(piperidin-4-yl)urea 71 (300 mg, 0.712 mmol) and tert-butyl 3 -oxoazetidine- 1- carboxylate 76 (122 mg, 0.712 mmol) in MeOH (10 mL) was added AcOH (0.081 mL, 1.43 mmol) and resulting reaction mass was stirred for Ih at same temperature followed by addition of NaCNBHa (89 mg, 1.43 mmol) in portion wise manner. The reaction was allowed to stir at 80°C for 16 h. After completion of reaction (TLC monitoring), solvent was evaporated up to dryness to get crude residue. The crude was further purified by flash column chromatography (silica gel, 40 g SNAP) using eluent 70% ethyl acetate in heptane to get the desired product as Off white solid 77 (350 mg, 85%) 'H NMR (400 MHz, DMSO-d6): 5 12.09 (s, 1H), 8.40 (s, 1H), 8.15 (m, 1H), 7.77-7.75 (d, J= 8.0 Hz, 2H), 7.43-7.41 (d, J= 8 Hz, 2H), 7.02 (s, 1H), 6.19-6.17 (d, J= 8Hz, 1H), 3.91-3.85 (m, 4H), 3.75-3.72 (m, 4H), 3.43 (s, 1H), 3.49 (s, 1H), 3.01 (m, 1H), 2.66-2.62 (m, 4H), 1.91-1.65 (m, 4H), 1.38 (s, 9H), 1.25-1.14 (m, 3H). LCMS= [M+H] ⁺ : 577.32, Purity= 89%.

Synthesis of l-(l-(azetidin-3-yl)piperidin-4-yl)-3-(4-(4-morpholino-7H-py rrolo[2,3-d]pyrimidin-6- yl)phenyl)urea (78):

To an ice cold stirred solution of tert-butyl 3-(4-(3-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl )ureido)piperi din- l-yl)azeti dine- 1 -carboxylate 77 (350 mg, 0.609 mmol) in DCM (10 mL), was added TFA (2.50 mL) in drop wise manner. The resulting reaction mass was stirred at room temperature for 2h. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure to get the crude residue, which was neutralized with Aq. NH4OH solution. The solid so precipitated out was collected by filtration and washed with water (2 x 25 mL) to get the desired product as yellowish solid 78 (250 mg, 86%). LCMS= [M+H] ⁺ : 477.20, Purity= 82.60%. Synthesis of l-(l-(l-acryloylazetidin-3-yl)piperidin-4-yl)-3-(4-(4-morpho lino-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)urea (Compound B0700-330):

[00700] To an ice cold stirred solution of l-(l-(azetidin-3-yl)piperidin-4-yl)-3-(4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)urea 78 (250 mg, 0.524 mmol) in DMF (5 mL) was added EtsN (0.21 mL, 1.57 mmol) and acryloyl chloride 27 (56 pL, 0.629 mmol). The resulting reaction mass was stirred at same temperature for next 30 minutes. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated up to dryness and the crude residue was purified through RP- HPLC purification eluted with 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column: Waters Xselect Phenyl-Hexyl(19*250mm,5pm) to get desired product as off white solid 330 (60 mg, 22%). 'H NMR (400 MHz, DMSO-d6 ): 8 11.85 (s, 1H), 8.29 (s, 1H), 8.16 (m, 1H), 7.75-7.73 (d, J= 8.0 Hz, 2H), 7.43-7.41 (d, J= 8Hz, 2H), 7.02 (s, 1H), 6.36-6.27 (m, 1H), 6.15-6.07 (m, 2H), 5.66-5.63 (m 1H), 3.91-3.85 (m, 7H), 3.75-3.72 (m, 4H), 3.62 (s, 1H), 3.56 (s, 1H), 3.40 (m, 1H), 2.71-2.66 (m, 2H), 2.07-2.02 (t, 2H), 1.88-1.85 (m, 2H) and 1.48-1.39 (m, 2H).

LCMS= [M+H] ⁺ : 531.28, Purity= 98.82%.

[00701] The following compound was or can be prepared using the appropriate reagents and the corresponding starting materials, and following the procedure described in Example B0700-10:

Example B0700-11

Synthesis of Compound B0700-331

Preparation of 2-(l-(l-acryloylazetidin-3-yl)piperidin-4-yl)-N-(4-(4-morpho lino-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)acetamide (Compound B0700-331):

Synthesis of tert-butyl 4-(2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)- 7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)amino)-2-oxoethyl)piperi dine-l-carboxylate (80):

[00702] To an ice cold stirred solution of 4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)aniline 7 (900 mg, 2.11 mmol) in DMF (10 mL) was added 2-(l-(tert- butoxycarbonyl)piperidin-4-yl)acetic acid 79 (875 mg, 3.59 mmol) and DIPEA (2.25 mL, 12.7 mmol) followed by the addition of T3P (6.72 mL, 10.60 mmol, 50% in ethyl acetate). The resulting reaction mixture was stirred at room temperature for 3h. After completion of reaction (TLC monitoring), solvent was concentrated up to dryness and purified by flash column chromatography (silica gel, 12 g SNAP) using 70% EtOAc in Hexane as eluent to get the desired product as off white solid 80 (600 mg, 44%).

'H NMR (400 MHz, DMSO-d6 ): 5 10.06 (s, 1H), 8.20 (s, 1H), 7.72-767 (m, 4H), 6.89 (s, 1H), 5.53 (s, 2H), 4.23-4.00 (m, 2H), 3.91-386 (m, 4H), 3.75-3.72 (m, 5H), 3.73-3.67 (m, 2H), 3.66-3.55 (t, 2H), 2.28-2.29 (m, 2H), 1.94-164 (m, 2H), 1.39 (s, 9H), 1.26-1.18 (m, 4H) and 0.07 (s, 9H). LCMS= [M+H] ⁺ : 651.36, Purity= 92%. Synthesis of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-2 -(piperidin-4- yl)acetamide (81):

[00703] To an ice cold solution of tert-butyl 4-(2-((4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)amino)-2- oxoethyl)piperidine-l -carboxylate 80 (600 mg, 0.799 mmol) in DCM (20 mL), added TFA (5.0 mL) and stirred at room temperature for 6h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue, which was neutralized with Aq. NH4OH solution. The solid so precipitated was collected by filtration and washed with water (2 x 25 mL) to get the desired product as yellowish solid 81 (250 mg, 66%).

'H NMR (400 MHz, DMSO-d6): 5 10.06 (s, 1H), 8.20 (s, 1H), 7.72-767 (m, 4H), 6.89 (s, 2H), 4.23- 4.00 (m, 2H), 3.91-3.86 (m, 4H), 3.75-3.72 (m, 4H), 2.73-2.68 (m, 4H), 1.3-1.24 (m, 4H).

LCMS= [M+H] ⁺ : 421.23, Purity= 80%.

Synthesis of tert-butyl 3-(4-(2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)amino)-2-oxoethyl)piperidin-l-yl)azetidine-l-carbo xylate (82):

[00704] To an ice cold stirred solution of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-2-(piperidin-4-yl)acetamide 81 (250 mg, 0.595 mmol) and tert-butyl 3 -oxoazetidine- 1- carboxylate 76 (153 mg, 0.892 mmol) in MeOH (10 mL) was added AcOH (0.067 mL, 1.19 mmol) and resulting reaction mixture was stirred at same temperature for Ih followed by addition of NaCNBHs (112 mg, 1.78 mmol). The reaction mass was allowed to stir at 80°C for 16h. After completion of reaction (TLC monitoring), solvent was evaporated up to dryness and the crude residue was diluted with ice-cold water (50 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (3 x 50 mL), dried over ISfeSCU, filtered and solvent was evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) using eluent 70% ethyl acetate in heptane to get the desired product as Off white solid 82 (250 mg, 73%)

LCMS= [M+H] ⁺ : 576.32) Purity= 80%.

Synthesis of 2-(l-(azetidin-3-yl)piperidin-4-yl)-N-(4-(4-morpholino-7H-py rrolo [2,3- d]pyrimidin-6-yl)phenyl)acetamide (83):

To an ice cold solution of tert-butyl 3-(4-(2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)amino)-2-oxoethyl )piperi din- l-yl)azeti dine- 1 -carboxylate 82 (250 mg, 0.434 mmol) in DCM (9 mL) was added TFA (1 mL) in drop wise manner. The resulting reaction mixture was allowed to stir at room temperature for 4h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue which was treated with Aq. NH4OH. The solid so precipitated out was collected by filtration and washed with water (2 x 20 mL) to get the desired product as yellowish solid 83 (250 mg, quantitative). LCMS= [M+H] ⁺ : 476.27, Purity= 49%.

Synthesis of 2-(l-(l-acryloylazetidin-3-yl)piperidin-4-yl)-N-(4-(4-morpho lino-7H-pyrrolo [2,3- d]pyrimidin-6-yl)phenyl)acetamide (Compound B0700-331):

[00705] To an ice cold stirred solution of 2-(l-(azetidin-3-yl)piperidin-4-yl)-N-(4-(4-morpholino- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)acetamide 83 (250 mg, 0.525 mmol) in DMF (5.0 mL), was added TEA (0.219 mL, 1.575 mmol) and acryloyl chloride 27 (56 pL, 0.629 mmol) and stirred for 30 min at same temperature. After completion of reaction (TLC monitoring), the resulting reaction mass was evaporated to dryness and the crude residue was purified through RP-HPLC purification eluted with 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column: Waters Xselect Phenyl- Hexyl(19*250mm,5pm) to get desired product as white solid 331 (160 mg, 58%).

¹ H NMR (400 MHz, DMSO-d6): 8 12.10 (s, 1H), 9.91 (s, 1H), 8.16 (m, 1H), 7.84-7.81 (d, J= 8.0 Hz, 2H), 7.65-7.63 (d, J= 8Hz, 2H), 7.02 (s, 1H), 6.36-6.27 (m, 1H), 6.15-6.07 (dd, 1H), 5.66-5.63 (dd, 1H), 4.23-4.00 (m, 1H), 3.91-3.85 (m, 2H), 3.91-386 (m, 4H), 3.75-3.72 (m, 4H), 3.73-3.67 (m, 1H), 3.10 (m, 1H), 2.80-2.73 (m, 2H), 2.26-2.24 (m, 2H), 1.82-1.66 (m, 5H), 1.26-1.18 (m, 2H). LCMS= [M+H] ⁺ : 530.28, Purity= 98%.

Example B0700-12

Synthesis of Compound B0700-332

[00706] Preparation of (S)-l-(l-(l-acryloylazetidin-3-yl)piperidin-3-yl)-3-(4-(4-mo rpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)urea (Compound B0700-332): Synthesis of tert-butyl (S)-3-(3-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methy l)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)ureido)piperidine-l-carb oxylate (85):

To a stirred solution of tert-butyl (3S)-3-aminopiperidine-l-carboxylate 84 (1.00 g, 4.99 mmol) in Dichloromethane (15 mL) was added CDI (1.62 g, 9.99 mmol) at room temperature and stirred for 16 h. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure to get crude (600 mg, colourless gel, -97% LCMS purity). The crude product was then added to a mixture of 4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7 H-pyrrolo[2,3- d]pyrimidin-6-yl]aniline 7 (2.13 g, 4.99 mmol) and triethylamine (683 pL, 4.99 mmol) in N,N- dimethylformamide (5 mL) at room temperature. The resultant reaction mixture was stirred at 90 °C for 16 h. After completion of reaction (TLC monitoring), reaction mixture was cooled at room temperature and quanched with ice cold water (50 mL) followed by extraction using EtOAc (3 X 50 mL). Combined organic layer was washed with brine (50 mL), dried over anhydrous Na2SO4 filtered and concentrated under reduced pressure and the residue was purified by silica-gel flash column chromatography (5-60% EtOAc/Heptane) to get desired product 85 (1.50 g, 99% ) as yellow solid. LCMS= [M+H] ⁺ : 652.36, Purity= 91%.

Synthesis of (S)-l-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)pheny l)-3-(piperidin-3- yl)urea (86):

[00707] To an ice cold solution of tert-butyl (S)-3-(3-(4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)ureido)piperidine-l- carboxylate 85 (1.50 g, 2.30 mmol) in DCM (40 mL) was added TFA (10 mL) in drop wise manner. The reaction mass was allowed to stir at room temperature for 4h. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure to get the crude residue which was neutralized with Aq. NH4OH solution. The solid so precipitated was collected by filtration and washed with water (2 x 25 mL) to get the desired product as yellowish solid 86 (900 mg, 92%).

'H NMR (400 MHz, DMSO-d6): 5 12.09 (s, 1H), 8.60 (s, 1H), 8.16 (s, 1H), 7.84 (d, J= 8Hz, 2H), 7.44 (d, J = 8Hz, 2H), 7.01 (s, 1H), 6.31 (s, 1H), 3.86 (m, 5H), 3.55 (m, 5H), 3.06 (m, 1H), 2.92 (m, 1H), 2.58-2.50 (m, 2H), 1.81-1.38 (m, 4H). LCMS= [M+H] ⁺ : 422.22, Purity= 75%

Synthesis of tert-butyl (S)-3-(3-(3-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)ureido)piperidin-l-yl)azetidine-l-carboxylate (87):

[00708] To an ice cold stirred solution of (S)-l-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-3-(piperi din-3 -yl)urea 86 (500 mg, 1.19 mmol) and tert-butyl 3 -oxoazetidine- 1- carboxylate 76 (305 mg, 1.78 mmol) in MeOH (15 mL) was added AcOH (0.136 mL, 2.37 mmol) and stirred for Ih at same temperature followed by addition of NaCNBHs (149 mg, 2.37 mmol). The resulting reaction mass was allowed to stir at 80°C for 16h. After completion of reaction (TLC monitoring), the resulting reaction mixture was evaporated up to dryness and the crude residue was diluted with ice-cold water (50 mL) followed by extraction using ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (3 x 50 mL), dried over ISfeSCU, filtered and solvent evaporated under reduced pressure to get the crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) using eluent 70% ethyl acetate in heptane to get the desired product as Off white solid 87 (550 mg, 66%).

LCMS= [M+H] ⁺ : 576.32, Purity= 83%.

Synthesis of (S)-l-(l-(azetidin-3-yl)piperidin-3-yl)-3-(4-(4-morpholino-7 H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)urea (88):

[00709] To an ice cold solution of tert-butyl (S)-3-(3-(3-(4-(4-morpholino-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)ureido)piperidin-l-yl)azetidine-l-ca rboxylate 87 (550 mg, 0.96 mmol) in DCM (18 mL) was added TFA (2 mL) in drop wise manner. The resulting reaction mass was stirred at room temperature for 6h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure to get the crude residue which was neutralized with Aq. NH4OH solution. The solid so precipitated was collected by filtration and washed with water (2 x 25 mL) to get the desired product as yellowish solid 88 (400 mg, 96.85%).

'H NMR (400 MHz, DMSO-d6): 512.09 (s, IH), 8.60 (bs, 2H), 8.16 (s, IH), 7.77 (d, J= 8Hz, 2H), 7.44 (d, J=8Hz, 2H), 7.01 (s, IH), 6.28 (m, IH), 3.92-3.68 (m, 13H), 3.40 (m, IH), 2.02-1.87 (m, 2H), 1.67-1.48 (m, 3H) and 1.10-0.83 (m, 3H). LCMS= [M+H] ⁺ : 477.26, Purity= 93%.

Synthesis of (S)-l-(l-(l-acryloylazetidin-3-yl)piperidin-3-yl)-3-(4-(4-mo rpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)urea (Compound B0700-332):

[00710] To an ice cold stirred solution of (S)-l-(l-(azetidin-3-yl)piperidin-3-yl)-3-(4-(4-morpholino- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)urea 88 (400 mg, 0.84 mmol) in DMF (10 mL), was added EtsN (0.35 mL, 2.517 mmol) and acryloyl chloride 27 (89 pL, 1.01 mmol) in drop wise manner. The resulting reaction mixture was stirred at room temperature for 30 minutes. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated up to dryness and the crude residue was purified through RP-HPLC purification eluted with 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column using Waters Xselect Phenyl-Hexyl(19*250mm,5pm) to get desired product as white solid 332 (190 mg, 43%). ¹ H NMR (400 MHz, DMSO-d6): 512.08 (s, 1H), 8.66 (d, J= 5.60 Hz), 8.15 (s, 1H), 7.77 (d, J= 8.0 Hz, 2H), 7.44 (d, J= 8.0 Hz, 2H), 7.01 (s, 1H), 6.35-6.26 (m, 2H), 6.12-6.07 (m, 1H), 5.60-5.64 (m, 1H), 4.26 (m, 1H), 4.02-3.92 (m, 2H), 3.87-3.86 (m, 5H), 3.75-3.69 (m, 6H), 3.17-3.11 (m, 1H), 2.32-2.13 (m, 3H), and 1.64-1.41 (m, 4H). LCMS= [M+H] ⁺ : 531.26, Purity= 97.84%.

Example B0700-12A

Synthesis of Compound B0700-333

Preparation of (R)-N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)pheny l)-4-((3- (vinylsulfonamido)piperidin-l-yl)methyl)picolinamide (333):

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7-( (2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)picolinamide (159): [00711] To a solution of tert-butyl N-[(3R)-l-{[2-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)pyridin-4- yl]methyl}piperi din-3 -yl]carbamate 9 (1.20 g, 1.62 mmol) in dichloromethane (20 mL) was added 4- m ethylbenzene- 1 -sulfonic acid hydrate (1.23 g, 6.46 mmol). The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring) the reaction mass was diluted with saturated solution of NaHCCh (50 mL) and extracted with DCM (3 x 50 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was triturated with diethyl ether and pentane to get desired product as pale yellow solid 159 (800 mg, 77%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 5 10.86 (s, 1H), 8.69 (d, J= 4.0 Hz, 1H), 8.27 (s, 1H), 8.12 (s, 1H), 8.08-8.05 (m, 2H), 7.78 (d, J= 8.0 Hz, 1H), 7.61-7.60 (m, 2H), 6.96 (s, 1H), 5.57 (s, 2H), 3.90-3.89 (m, 4H), 3.75-7.71 (m, 4H), 3.60-3.52 (m, 4H), 2.67-2.62 (m, 3H), 1.97 (m, 3H), 1.74-1.69 (m, 4H), 1.22 (m, 1H), 0.89-0.85 (m, 2H) and 0.06- 0.01 (m, 9H). LCMS: [M+H] ⁺ : 643.45, Purity= 98.55%.

Synthesis of (R)-N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)- 7H-pyrrolo [2,3- d]pyrimidin-6-yl)phenyl)-4-((3-(vinylsulfonamido)piperidin-l -yl)methyl)picolinamide (161): [00712] To an ice cold solution of 4-{[(3R)-3-aminopiperidin-l-yl]methyl}-N-{4-[4-(morpholin-4- yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]py rimidin-6-yl]phenyl}pyridine-2- carboxamide 159 (500 mg, 778 pmol) in N,N-dimethylformamide (5 mL) was added tri ethylamine (236 mg, 2.33 mmol) and ethenesulfonyl chloride 160 (118 mg, 933 pmol) in drop wise. The resulting reaction mixture was stirred for 16h at room temperature. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was evaporated up to complete dryness. The crude residue was purified through column chromatography (silica gel, 12 g SNAP) eluted with 2-3% MeOH:DCM to get desired product as lighy brown solid 161 (150 mg, 26%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 8 10.83 (s, 1H), 8.70 (d, J= 8.0 Hz, 1H), 8.27 (s, 1H), 8.11 (s, 1H), 8.08 (d, J= 8.0 Hz, 2H), 7.79 (d, J= 8.0 Hz, 2H), 7.62 (d, J= 4.0 Hz, 1H), 7.40 (d, J= 8.0 Hz, 2H), 6.96 (s, 1H), 6.75-6.69 (m, 1H), 6.03-5.99 (m, 1H), 5.88-5.86 (m, 1H), 5.58 (s, 2H), 3.90-3.88 (m, 4H), 3.75-3.73 (m, 4H), 3.65-6.61 (m, 3H), 3.15-3.12 (m, 2H), 2.85-2.83 (m, 3H), 2.50-2.48 (m, 2H), 1.98-1.93 (m, 1H), 1.81-1.79 (m, 1H), 1.65-1.62 (m, 1H), 0.87-0.85 (m, 1H) and 0.069 (s, 9H). LCMS: [M+H] ⁺ : 733.40, Purity= 99.44%.

Synthesis of (R)-N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)-4-((3-

(vinylsulfonamido)piperidin-l-yl)methyl)picolinamide (333):

[00713] An ice cold solution of (R)-N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)- 7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-4-((3 -(vinyl sulfonamido)piperidin-l-yl)methyl)picolinamide 161 (80 mg, 109 pmol) in 25% TFA in di chloromethane (5.0 mL) was allowed to stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude was neutralized with aqueous NH4OH solution and extracted with 20% IPA: CHCL (2 x 25 mL). The combined organic layer was dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by column chromatography (silica gel, 12 g SNAP) using eluents 3% MeOH in DCM to get the desired product as light green solid 333 (35 mg, 27%).

¹ H NMR (400 MHz, DMSO-t/ ₆ ): 8 12.21 (s, 1H), 10.75 (s, 1H), 8.69 (d, J = 4.8 Hz, 1H), 8.18 (s, 1H), 8.10 (s, 1H), 8.01 (d, J= 8.4 Hz, 2H), 7.92 (d, J= 8.8 Hz, 2H), 7.61-7.60 (m, 1H), 7.40 (d, J= 8.4 Hz, 1H), 7.16 (s, 1H), 6.75-6.69 (m, 1H), 6.03 (d, J= 16.8 Hz, 1H), 5.88 (d, J= 9.6 Hz, 1H), 3.88-3.87 (m, 4H), 3.76-3.75 (m, 4H), 3.64 (s, 2H), 3.15 (m, 1H), 2.85-2.83 (m, 1H), 2.62-2.59 (m, 1H), 1.93 (m, 2H), 1.81-179 (m, 1H), 1.70-1.68 (m, 1H), 1.48-1.45 (m, 1H) and 1.09-1.01(m,lH). LCMS: [M+H] ⁺ : 603.30, Purity= 97.96%.

Example B0700-12B

Synthesis of Compound B0700-335

Preparation of (R)-4-((3-(2,5-dioxo-2,5-dihydro-lH-pyrrol-l-yl)piperidin-l- yl)methyl)-N-(4-(4- morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinami de (335):

[00714] A solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide 10 (400 mg, 780 pmol) and 2, 5 -dihydrofuran -2, 5-dione 162 (115 mg, 1.5 eq., 1.17 mmol) in acetic acid (10 mL) was heated at 100°C for 16h. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated up to dryness. The crude residue was purified through RP-HPLC purification using 5mM ammonium bicarbonate in water/ acetonitrile (Waters XB ridge C-8(19*250mm,5pm) flow rate Acetonitrile (%)-35-70%, Flow-18. Oml/min, 16 min to get desired product as yellow solid 335 (40 mg, 8.3%).

¹ H NMR (400 MHz, DMSO-t/ ₆ : > 12.20 (s, 1H), 10.74 (s, 1H), 8.68 (d, J = 4.8 Hz, 1H), 8.18 (s, 1H), 8.11 (s, 1H), 8.01 (d, J = 8.8 Hz, 2H) 7.92 (d, J = 8.4 Hz, 2H), 7.61 (d, J = 4.0 Hz, 1H), 7.16 (s, 1H), 6.95 (s, 2H), 4.02-3.96 (m, 1H), 3.90-3.87 (m, 4H), 3.76-3.74 (m, 4H), 3.69-3.64 (m, 2H), 2.80-2.77 (m, 1H), 2.72-2.69 (m, 1H), 2.55-2.53 (m, 1H), 2.01-1.93 (m, 2H), 1.74-1.71 (m, 2H) and 1.57-1.54 (m, 1H). LCMS: [M+H] ⁺ : 593.03, Purity= 96.97%. Example B0700-12C

Synthesis of Compound B0700-336

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(piperidi n-l-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (336):

Synthesis of tert-butyl (R)-(l-((2-((4-(4-(piperidin-l-yl)-7-((2-(trimethylsilyl)eth oxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)m ethyl)piperidin-3-yl)carbamate (89):

[00715] To a solution of tert-butyl (R)-(l-((2-((4-(4-chloro-7-((2-(trimethylsilyl)ethoxy)methyl )-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)m ethyl)piperidin-3-yl)carbamate 89 (300 mg, 0.43 mmol) and piperidine (129 pL, 1.30 mmol) in DMSO (2.00 mL) was added DIPEA (227 pL, 1.30 mmol). The reaction mixture was stirred at 120°C for Ih. After completion of reaction (TLC and LCMS), reaction mixture was diluted with ice cold water (50 m) and extracted with ethyl acetate (2x 50 mL). The combined organic layer was washed with brine solution, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude was purified over flash column chromatography in silica gel (230-400M) eluted with 50% EtOAc in heptane to get desired product as light brown solid 164 (285 mg, 86.09%).

LCMS: [M+H] ⁺ : 741.53, Purity= 99.15%.

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-(piperidin-l-y l)-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide (165):

[00716] To an ice cold stirred solution of tert-butyl N-[(3R)-l-{[2-({4-[4-(piperidin-l-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}carbamoyl)pyridin-4-yl]m ethyl}piperidin-3-yl]carbamate 164 (280 mg, 458 pmol) in DCM (15.0 mL) was added TFA (10.0 mL). The resulting reaction mixture was stirred at room temperature for 16h. After completion reaction (TLC and LCMS monitoring), reaction mixture was concentrated under reduced pressure. The crude was triturated with diethyl ether to get desired product as off white solid 165 (248 mg, quantitative).

LCMS: [M+H] ⁺ : 511.03, Purity= 83.78%.

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(piperidi n-l-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (336):

[00717] To an ice cold stirred solution of 4-{[(3R)-3-aminopiperidin-l-yl]methyl}-N-{4-[4- (piperidin-l-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}pyri dine-2-carboxamide 165 (160 mg, 313 pmol) in DMF (5.0 mL) was added triethylamine (131 pL, 940 pmol) and prop-2-enoyl chloride 27 (20.3 pL, 251 pmol) in drop wise. The resulting reaction mixture was stirred at same temperature for 30 min. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was concentrated under reduced pressure. The crude residue was purified through flash column chromatography eluted with 3-4% MeOH in DCM to get desired product as pale yellow solid 336 (35 mg, 22%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 12.10 (br s, 1H), 10.75 (br s, 1H), 8.68 (d, J= 8.0 Hz, 1H), 8.13 (s, 1H), 8.11 (s, 1H), 8.00-7.97 (m, 2H), 7.92-7.89 (m, 2H), 7.63 (d, J= 8.0 Hz, 2H), 7.07 (s, 1H), 6.25- 6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57-5.54 (m, 1H), 3.91-3.89 (m, 4H), 3.82 (m, 1H), 3.65 (s, 2H), 2.79-2.77 (m, 1H), 2.66-2.64 (m, 1H), 2.06-2.01 (m, 1H), 1.92-1.87 (m, 1H), 1.86-1.80 (m, 8H), 1.55- 1.52 (m, 1H) and 1.33-1.31 (m, 1H). LCMS: [M+H] ⁺ : 565.12, Purity= 99.78%.

Example B0700-12D

Synthesis of Compound B0700-337

Preparation of (R)-N-(4-(4-(4,7-diazaspiro[2.5]octan-7-yl)-7H-pyrrolo[2,3-d ]pyrimidin-6- yl)phenyl)-4-((3-acrylamidopiperidin-l-yl)methyl)picolinamid e (337): Synthesis of tert-butyl (R)-(l-((2-((4-(4-(4,7-diazaspiro[2.5]octan-7-yl)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3-yl)carbamate (167):

[00718] To a solution of tert-butyl (R)-(l-((2-((4-(4-chloro-7-((2-(trimethylsilyl)ethoxy)methyl )-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)m ethyl)piperidin-3-yl)carbamate 89 (320 mg, 0.46 mmol) and 4,7-diazaspiro[2.5]octane dihydrochloride 166 (171 mg, 0.93 mmol) in DMSO (2.0 mL) was added DIPEA (404 pL, 2.31 mmol). The reaction mixture was heated at 120°C for Ih. After completion of reaction (TLC and LCMS), reaction mixture was diluted with ice cold water (50 mL) and extracted with ethyl acetate (2 x 50 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude was purified by flash column chromatography in silica gel (230-400M) eluted with 50% EtOAc in heptane to get desired product as light brown solid 167 (260 mg, 72.09%).

'H NMR (400 MHz, DMSO-t/ ₆ ): > 10.81 (s, IH), 8.69-8.68 (m, IH), 8.20 (s, IH), 8.11 (s, IH), 8.07- 8.05 (d, J = 8.0 Hz, 2H), 7.77 (d, J = 8.0 Hz, 2H), 7.62-7.61 (m, 2H), 6.86 (s, IH), 6.76-6.74 (m, IH), 5.55 (s, 2H), 3.91 (m, 2H), 3.68 (s, 2H), 3.63-3.61 (m, 4H), 3.43-3.41 (m, 2H), 2.87 (m, 2H), 2.77- 2.75 (m, 2H), 2.53 (m, 4H), 1.97-1.92 (m, 2H), 1.84-1.83 (m, 2H), 1.69-1.62 (m, 2H), 1.48 (m, 2H), 1.20-1.19 (m, 2H), 0.88-0.84 (m, 2H), 0.55 (s, 2H), 0.44 (s, 2H) and -0.076 (s, 9H).

LCMS: [M+H] ⁺ : 768.10, Purity= 98.00%.

Synthesis of (R)-N-(4-(4-(4,7-diazaspiro [2.5] octan-7-yl)-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)-4-((3-aminopiperidin-l-yl)methyl)picolinamide (168):

[00719] To an ice cold solution of tert-butyl N-[(3R)-l-[(2-{[4-(4-{4,7-diazaspiro[2.5]octan-7-yl}- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl]carbamoyl}pyridin-4-y l)methyl]piperidin-3-yl]carbamate 167 (250 mg, 0.392 mmol) in DCM (10.0 mL) was added TFA (15.0 mL). The resulting reaction mixture was stirred at room temperature for 16h. After completion reaction (TLC and LCMS monitoring), reaction mixture was concentrated under reduced pressure to get crude and triturated with diethyl ether to get desired product as off white solid 167 (220 mg, 98%). LCMS: [M+H] ⁺ : 538.22, Purity= 87.70%.

Synthesis of (R)-N-(4-(4-(4,7-diazaspiro [2.5] octan-7-yl)-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)-4-((3-acrylamidopiperidin-l-yl)methyl)picolinamid e (337):

[00720] To an ice cold solution of (R)-N-(4-(4-(4,7-diazaspiro[2.5]octan-7-yl)-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)-4-((3-aminopiperidin-l-yl)methyl)pi colinamide 168 (140 mg, 260 pmol) in DMF (15.0 mL) was added triethylamine (109 pL, 781 pmol) and prop-2-enoyl chloride 27 (16.8 pL, 208 pmol) drop wise. The resulting reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was evaporated under reduced pressure. The crude residue was purified through column chromatography in silica gel (230-400M, 12g SNAP) using eluents 3-4% MeOH in DCM to get desired product as pale yellow solid 337 (12.0 mg, 9%).

¹ H NMR (400 MHz, DMSO-t/ ₆ ): 8 12.13 (s, 1H), 10.73 (s, 1H), 8.69 (d, J = 5.2 Hz, 1H), 8.11 (s, 2H), 8.00-7.98 (m, 2H), 7.92 (d, J = 8.4 Hz, 2H), 7.63 (d, J = 9.2 Hz, 1H), 7.05 (s, 1H), 6.25-6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57 (dd, J= 2.0 & 1.0 Hz, 1H), 3.91-3.90 (m, 2H), 3.90-3.86 (m, 1H), 3.76 (s, 2H), 3.66 (s, 2H), 2.90-2.89 (m, 2H), 2.79-2.77 (m, 1H), 2.66-2.64 (m, 1H), 2.06-2.01 (m, 1H), 1.92- 1.87 (m, 1H), 1.77-1.70 (m, 3H), 1.55-1.52 (m, 1H), 1.24-1.19 (m, 2H), 0.59 (s, 2H) and 0.47 (s, 2H). LCMS: [M+H] ⁺ : 592.44, Purity= 95.87%.

Example B0700-12E

Synthesis of Compound B0700-338

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(4-cyclop ropylpiperazin-l- yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (338): Preparation of tert-butyl (R)-(l-((2-((4-(4-(4-cyclopropylpiperazin-l-yl)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3-yl)carbamate (170):

[00721] To a solution of tert-butyl (R)-(l-((2-((4-(4-chloro-7-((2-(trimethylsilyl)ethoxy)methyl )-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)m ethyl)piperidin-3-yl)carbamate

89 (300 mg, 433 pmol) and 1 -cyclopropylpiperazine 169 (164 mg, 1.30 mmol) in DMSO (2.0 mL) was added DIPEA (227 pL, 1.30 mmol). The reaction mixture was stirred at 120°C for Ih. After completion of reaction (TLC and LCMS), reaction mixture was diluted with ice cold water (50 mL) and extracted with ethyl acetate (2 x 50 mL). The combined organic layer was washed with brine solution, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude was purified by flash column chromatography in silica gel (230-400M) eluted with 50% EtOAc in heptane to get desired product as light brown solid 170 (305 mg, 88%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): > 10.82 (s, IH), 8.69-8.68 (m, IH), 8.24 (s, IH), 8.11 (s, IH), 8.07 (d, J = 8.0 Hz, 2H), 7.78 (d, J = 8.0 Hz, 2H), 7.62 (d, J = 4.0 Hz, 2H), 6.92 (s, IH), 6.76-6.74 (m, IH), 5.56 (s, 2H), 3.88-3.86 (m, 4H), 3.65-3.61 (m, 4H), 3.40 (m, 4H), 3.75 (m, 2H), 2.67-2.65 (m, 4H), 1.97-1.92 (m, 3H), 1.84-1.83 (m, 2H), 1.69-1.62 (m, 3H), 1.48 (m, 2H), 1.20-1.19 (m, 2H), 0.88- 0.84 (m, 2H), 0.55 (s, 2H), 0.44 (s, 2H) and -0.076 (s, 9H).

LCMS: [M+H] ⁺ : 782.02, Purity= 98.00%.

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-(4-cyclopropyl piperazin-l-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (171):

[00722] To an ice cold solution of tert-butyl (R)-(l-((2-((4-(4-(4-cyclopropylpiperazin-l-yl)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyri din-4- yl)methyl)piperidin-3-yl)carbamate 170 (300 mg, 460 pmol) in DCM (10.0 mL) was added TFA (5.0 mL). The resulting reaction mixture was stirred at room temperature for 16h. After completion reaction (TLC and LCMS monitoring), reaction mixture was concentrated under reduced pressure. The crude was triturated with diethyl ether to get desired product as off white solid 171 (180 mg, 85%). LCMS: [M+H] ⁺ : 552.40, Purity= 99%.

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(4-cyclop ropylpiperazin-l-yl)- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (338):

[00723] To an ice-cold solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-(4- cyclopropylpiperazin-l-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)p henyl)picolinamide 171 (180 mg, 326 pmol) in DMF (15.0 mL) was added EtsN (136 pL, 979 pmol) and prop-2-enoyl chloride 27 (21.1 pL, 261 pmol). The resulting reaction mixture was stirred at 0°C for 10 min. After completion of reaction (TLC monitoring). The reaction mixture was diluted with ice cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crdue purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 4% MeOH in DCM to get desired product as pale yellow solid 338 (25 mg, 13%).

¹ H NMR (400 MHz, DMSO-t/ ₆ ): 5 12.16 (br s, 1H), 10.74 (br s, 1H), 8.69 (d, J = 8.8 Hz, 1H), 8.15 (s, 1H), 8.11 (s, 1H), 8.01-7.98 (m, 2H), 7.92 (d, J= 8.8 Hz, 2H), 7.63 (d, J= 4.4 Hz, 1H), 7.13 (s, 1H), 6.25-6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57 (dd, J = 2.0 & 10.0 Hz, 1H), 3.86 (m, 5H), 3.66 (s, 2H), 2.79-2.77 (m, 1H), 2.70-2.66 (m, 5H), 2.06-2.01 (m, 1H), 21.92-1.87 (m, 1H), 1.78-1.68 (m, 3H), 1.55-1.49 (m, 1H), 1.22-1.16 (m, 2H), 0.47-0.45 (m, 2H) and 0.41-0.39 (m, 2H).

LCMS: [M+H] ⁺ : 606.14, Purity= 95.14%.

Example B0700-12F

Synthesis of Compound B0700-341

Preparation of 4-(((R)-3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-((S)-3-me thylmorpholino)- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (341):

Preparation of tert-butyl ((R)-l-((2-((4-(4-((S)-3-methylmorpholino)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3-yl)carbamate (173): [00724] To a solution of tert-butyl N-[(3R)-l-[(2-{[4-(4-chloro-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl]carbamoyl}pyri din-4- yl)methyl]piperidin-3-yl]carbamate 89 (300 mg, 433 pmol) in DMSO (2.0 mL) was added (3S)-3- methylmorpholine 172 (131 mg, 1.30 mmol) and DIPEA (227 pL, 1.30 mmol). The reaction mixture was stirred at 120°C for Ih. After completion of reaction (TLC and LCMS), reaction mixture was diluted with ice cold water (50 mL) and extracted with ethyl acetate (2x 50 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude was purified by flash column chromatography in silica gel (230-400 M) eluted with 50% EtOAc in heptane to get desired product as light brown solid 173 (320 mg, 98%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 8 10.82 (s, IH), 8.70-8.68 (m, IH), 8.24 (s, IH), 8.26 (s, IH), 8.08- 8.05 (m, 2H), 7.78 (d, J = 8.0 Hz, 2H), 7.62-7.61 (d, J = 4.0 Hz, 2H), 6.92 (s, IH), 6.76-6.74 (m, IH), 5.57 (s, 2H), 4.79 (m, IH), 4.44-4.41 (m, IH), 3.99 (m, IH), 3.83-3.81 (m, 2H), 3.81-3.60 (m, 4H), 3.55 (m, IH), 3.45 (m, IH), 2.79-2.75 (m, 2H), 2.66-2.64 (m, IH), 1.94-1.73 (m, 4H), 1.55 (m, IH), 1.35 (s, 9H), 1.29-1.28 (m, 3H), 0.89-0.87 (m, 2H) and -0.077 (s, 9H).

LCMS: [M+H] ⁺ : 757.24, Purity= 92.66%.

Synthesis of 4-(((R)-3-aminopiperidin-l-yl)methyl)-N-(4-(4-((S)-3-methylm orpholino)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (174):

[00725] To an ice cold solution of tert-butyl N-[(3R)-l-({2-[(4-{4-[(3R)-3-methylmorpholin-4-yl]- 7H-pyrrolo[2,3-d]pyrimidin-6-yl}phenyl)carbamoyl]pyridin-4-y l}methyl)piperidin-3-yl]carbamate 173 (350 mg, 558 pmol) in DCM (10.0 mL) was added TFA (15.0 mL). The resulting reaction mixture was stirred at room temperature for 5h. After completion reaction (TLC and LCMS monitoring), reaction mixture was concentrated under reduced pressure. The crude was triturated with diethyl ether to get desired product as brown viscous liquid 174 (260 mg, 91%). LCMS: [M+H] ⁺ : 527.21, Purity= 87%.

Synthesis of 4-(((R)-3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-((S)-3-me thylmorpholino)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (341):

[00726] To an ice-cold solution of 4-(((R)-3-aminopiperidin-l-yl)methyl)-N-(4-(4-((S)-3- methylmorpholino)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)pic olinamide 174 (220 mg, 418 pmol) in DMF (15.0 mL) was added EtsN (175 pL, 3 eq., 1.25 mmol). The resulting solution was stirred at same temperature for 10 min, followed by addition of prop-2-enoyl chloride 27 (27.0 pL, 0.8 eq., 334 pmol). The resulting reaction mixture was stirred at 0°C for 20 min. After completion of reaction (TLC monitoring), the reaction mass was diluted with ice cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude was purified by RP-HPLC using 5mM ammonium bicarbonate in water/acetonitrile and column Waters XBridge C- 8(19*250mm,5pm) to get desired product as yellow solid 341 (46 mg, 18%).

¹ H NMR (400 MHz, DMSO-t/r,): 8 12.18 (s, 1H), 10.74 (s, 1H), 8.68 (d, J = 4.0 Hz, 1H), 8.17 (s, 1H), 8.11 (s, 1H), 8.01-7.98 (m, 2H), 7.92 (d, J= 8.0 Hz, 2H), 7.63 (d, J= 4.0 Hz, 1H), 7.12 (s, 1H), 6.25- 6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57-5.54 (m, 1H), 4.81-4.79 (m, 2H), 4.44-4.40 (m, 1H), 3.98-3.96 (m, 1H), 3.89 (m, 1H), 3.81-3.79 (m, 2H), 3.66 (s, 2H), 3.55-3.51 (m, 1H), 3.40-3.47 (m, 1H), 2.79- 2.77 (m, 1H), 2.66-2.64 (m, 1H), 2.06-2.01 (m, 1H), 1.92-1.87 (m, 1H), 1.78-1.78 (m, 2H), 1.55-1.52 (m, 1H), 1.29-1.28 (m, 3H) and 1.23-1.19 (m, 1H). LCMS: [M+H] ⁺ : 581.09, Purity= 99.78%.

Example B0700-12G

Synthesis of Compound B0700-342

Preparation of (R)-4-((3-(but-2-ynamido)piperidin-l-yl)methyl)-N-(4-(4-morp holino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (342):

[00727] To an ice-cold solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide 10 (525 mg, 1.02 mmol) in DMF (10.0 mL) was added but-2-ynoic acid 175 (103 mg, 1.23 mmol), DIPEA (397 mg, 3.07 mmol) and HATU (779 mg, 2.05 mmol). The resulting reaction mixture was stirred at RT for Ih. After the completion of the reaction (monitored by TLC), reaction mixture was diluted with cold water (100 mL) and extracted with EtOAc (2 x 100 mL). The combined organic part was dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified over flash column chromatography (silica gel, 230-400 M, 40 g SNAP) eluted with 3-6% MeOH in DCM to get desired product 342 as light yellow solid (370 mg, 62%). ^X H NMR (400 MHz, DMSO-t/ ₆ ): 5 12.20 (s, 1H), 10.74 (s, 1H), 8.68 (d, J= 4.0 Hz, 1H), 8.43 (d, J= 8.0 Hz, 1H), 8.18 (s, 1H), 8.10 (s, 1H), 8.01-7.98 (m, 2H), 7.92 (d, J = 8.0 Hz, 2H), 7.61 (d, J= 4.0 Hz, 1H), 7.16 (s, 1H), 3.89-3.86 (m, 4H), 3.76-3.74 (m, 5H), 3.64 (s, 1H), 2.76-2.74 (m, 2H), 2.66- 2.64 (m, 1H), 2.10-1.97 (m, 1H), 1.93 (s, 3H), 1.74-1.63 (m, 2H), 1.56-1.51 (m, 1H) and 1.30-1.26 (m, 2H). LCMS: [M+H] ⁺ : 579.11, Purity= 99.56%.

Example B0700-13

Synthesis of Compound B0700-304

[00728] Compound B0700-304 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein: Example B0700-14

Synthesis of Compound B0700-305

[00729] Compound B0700-305 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein: Example B0700-15

Synthesis of Compound B0700-307

[00730] Compound B0700-307 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein:

Example B0700-16

Synthesis of Compound B0700-322

[00731] Compound B0700-322 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein:

Example B0700-17

Synthesis of Compound B0700-326

[00732] Compound B0700-326 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein:

Example B0700-18

Synthesis of Compound B0700-327

[00733] Compound B0700-327 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein: Example B0700-22

Synthesis of Compounds B0700-339 and B0700-340

[00734] Compounds 339 and 340 were prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein: Example B0700-25

Synthesis of Compound B0700-343

[00735] Compound B0700-343 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein: Example B0700-27C

Synthesis of Compound B0700-507

Preparation of N-(l-(2-((7-(6-morpholino-9H-purin-8-yl)-7-azaspiro[3.5]nona n-2- yl)amino)pyridin-4-yl)azetidin-3-yl)acrylamide (507):

Synthesis of 7-azaspiro[3.5]nonan-2-one (124):

[00736] To an ice cold stirred solution of tert-butyl 2-oxo-7-azaspiro[3.5]nonane-7-carboxylate 123 (3.20 g, 13.4 mmol) in 1,4-dioxane (20 mL) was added 4 M HC1 in dioxane (16.7 mL, 66.9 mmol). The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring) the reaction mixture was concentrated under reduced pressure and the crude residue was triturated with diethyl ether (2 x 50 mL) to get the desired product 124 (2.10 g as HC1 salt) as white solid.

'H NMR (400 MHz, DMSO-t/ ₆ ): 5 11.2 (br s, 2H), 3.00 (s, 4H), 2.89 (s, 4H) and 1.89-1.86 (m, 4H).

Synthesis of 7-(6-morpholino-9-((2-(trimethylsilyl)ethoxy)methyl)-9H-puri n-8-yl)-7- azaspiro[3.5]nonan-2-one (125):

[00737] To a solution of 8-bromo-6-(morpholin-4-yl)-9-{[2-(trimethylsilyl)ethoxy]meth yl}-9H- purine 51 (1.00 g, 2.41 mmol) in 1,4-dioxane (30 mL) was added 7-azaspiro[3.5]nonan-2-one 124 (504 mg, 1.5 eq., 3.62 mmol) and CS2CO3 (2.36 g, 3 eq., 7.24 mmol). The resulting reaction mixture was degassed with nitrogen for 15 minutes followed by addition of Pd2(dba)3 (221 mg, 241 pmol) and Xantphos (279 mg, 483 pmol). The reaction mixture was heated at 100°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed and washed with ethyl acetate (50 mL). The organic part was diluted with water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 120 g SNAP) eluted with 10% ethyl acetate in heptane to get desired product as red solid 125 (150 mg, 13%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.24 (s, 1H), 5.59 (s, 2H), 4.19 (br s, 4H), 3.69-3.65 (m, 6H), 3.56 (m, 4H), 2.84 (s, 4H), 1.70-1.61 (m, 4H), 0.91-0.87 (m, 2H) and 0.05 (s, 9H).

Synthsis of tert-butyl (l-(2-((7-(6-morpholino-9-((2-(trimethylsilyl)ethoxy)methyl) -9H-purin-8- yl)-7-azaspiro [3.5] nonan-2-yl)amino)pyridin-4-yl)azetidin-3-yl)carbamate (126) :

[00738] To an ice-cold solution of 7-[6-(morpholin-4-yl)-9-{[2-(trimethylsilyl)ethoxy]methyl}-9 H- purin-8-yl]-7-azaspiro[3.5]nonan-2-one 125 (150 mg, 317 pmol) and tert-butyl N-[l-(2- aminopyridin-4-yl)azetidin-3-yl]carbamate 107 (83.9 mg, 317 pmol) in THF (15.0 mL) was added titanium (IV) isopropoxide (282 pL, 952 pmol) dropwise. The reaction mixture was stirred at 90 °C for 16h. After completion of starting material, reaction mixture was cooled at 0°C and sodium cyanoborohydride (40 mg, 635 pmol) was added. The resulting reaction mixture was stirred at room temperature for 3h. After completion of reaction (TLC monitoring), reaction mixture was cooled to 0°C, diluted with water (100 mL) and extracted with 20% MeOH:DCM (5 x 100 mL). The combined organic layer was washed with brine, dried over anhydrous Na2SC>4, filtered and concentrated under reduced pressure. The crude residue was purified by silica-gel (100-200M) flash column chromatography, elution with 2-3% MeOH in DCM to get desired product as yellow solid 126 (80 mg, 35%). LCMS: [M+H] ⁺ : 721.31, Purity= 58%.

Synthsis of N-(4-(3-aminoazetidin-l-yl)pyridin-2-yl)-7-(6-morpholino-9H- purin-8-yl)-7- azaspiro[3.5]nonan-2-amine (127):

[00739] To an ice cold solution of tert-butyl N-{ l-[2-({7-[6-(morpholin-4-yl)-9-{[2- (trimethylsilyl)ethoxy]methyl}-9H-purin-8-yl]-7-azaspiro[3.5 ]nonan-2-yl}amino)pyri din-4- yl]azetidin-3-yl}carbamate 126 (80 mg, 111 pmol) in DCM (5.0 mL) was added TFA (2.0 mL). The resulting reaction mixture was allowed to stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude residue was neutralized with aqueous NH4OH solution and extracted with 20% IPA in CHCL (2 x 50 mL). The combined organic layer was dried over anhydrous Na2SC>4, filtered and concentrated under reduced pressure to get the desired product as brown solid 127 (60 mg, quantitative). LCMS: [M+H] ⁺ : (491.08), Purity= 50%. Synthesis of N-(l-(2-((7-(6-morpholino-9H-purin-8-yl)-7-azaspiro[3.5]nona n-2- yl)amino)pyridin-4-yl)azetidin-3-yl)acrylamide (507):

[00740] To an ice cold stirred solution of N-[4-(3-aminoazetidin-l-yl)pyridin-2-yl]-7-[6- (morpholin-4-yl)-9H-purin-8-yl]-7-azaspiro[3.5]nonan-2-amine 127 (60 mg, 122 pmol) in DMF (2.0 mL) was added EtsN (50.2 pL, 3 eq., 367 pmol) and acryloyl chloride 27 (4.43 mg, 0.4 eq., 48.9 pmol) drop wise. The resulting reaction mixture was stirred at same temperature for 30 minutes. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated up to dryness and the crude residue was purified through RP-HPLC purification eluted with 5mM ammonium bicarbonate in water/acetonitrile (Waters XSelect CSH C-18(19*250mm,10pm) get desired product as white solid 507 (4 mg, 6%).

¹ H NMR (400 MHz, DMSO-t/ ₆ ): 5 12.10 (s, 1H), 8.77-8.75 (m, 1H), 8.00 (s, 1H), 7.62 (d, J= 4.0 Hz, 1H), 6.27-6.08 (m, 3H), 5.71-5.69 (m, 1H), 5.65-5.62 (m, 1H), 5.31 (s, 1H), 4.66-4.65 (m, 1H), 4.19- 4.17 (m, 1H), 4.13-4.10 (m, 2H), 4.02 (br s, 4H), 3.69-3.66 (m, 4H), 3.62-3.61 (m, 2H), 3.44 (s, 2H), 3.35-3.34 (m, 2H), 2.26-2.22 (m, 2H) and 1.62-1.59 (m, 6H).

LCMS: [M+H] ⁺ : 545.12, Purity= 90%.

Example B0700-28

Synthesis of Compound B0700-509

[00741] Compound B0700-509 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-N-(l-(3-(N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6 - yl)phenyl)sulfamoyl)benzyl)piperidin-3-yl)acrylamide (509): Synthesis of methyl 3-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7 H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)sulfamoyl)benzoate (141):

[00742] To an ice cold solution of 4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7 H- pyrrolo[2,3-d]pyrimidin-6-yl] aniline 7 (1.80 g, 4.23 mmol) in DCM (20 mL) was added pyridine (1.02 mL, 12.7 mmol). The resulting reaction mixture was stirred for 15 minutes at same temperature followed by addition of a solution of methyl 3-(chlorosulfonyl)benzoate 140 (992 mg, 4.23 mmol) in DCM (1.0 mL). The resulting reaction mixture was stirred at RT for 3h. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice-cold water (100 mL) and extracted with DCM (2 x 200 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and solvent was evaporated under reduced pressure to get the desired product as brown viscous 141 (2.0 g, 67%).

'H NMR (400 MHz, DMSO-d6): 5 10.67 (s, 1H), 8.35 (s, 1H), 8.24 (s, 1H), 8.06-8.04 (m, 1H), 7.76- 7.72 (m, 1H), 7.64-7.62 (m, 2H), 7.19-7.17 (m, 2H), 6.87 (s, 1H), 5.48 (s, 2H), 3.88 (s, 3H), 3.85 (m, 4H), 3.71-3.70 (m, 4H), 3.55-3.51 (m, 2H), 0.82-0.78 (m, 3H) and 0.13 (s, 9H).

LCMS: [M+H] ⁺ : 624.09, Purity= 87%.

Synthesis of 3-(hydroxymethyl)-N-(4-(4-morpholino-7-((2-(trimethylsilyl)e thoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)benzenesulfonamide (142):

[00743] To an ice-cold solution of methyl 3-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}sulfamoyl)benzoate 141 (1.0 g, 1.60 mmol) in THF (10.0 mL) was added LAH (2.0 M in THF, 1.20 mL, 2.40 mmol) dropwise. The resulting reaction mixture was stirred at 0°C for next 2h under N2 atmosphere. After completion of reaction (TLC monitoring), the reaction was quenched with aqueous solution of NH4CI (50 mL) and extracted with ethyl acetate (2 x 100 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get desired product as light brwon viscous 142 (800 mg, 79%).

'H NMR (400 MHz, DMSO-d6): 5 10.54 (s, 1H), 8.24 (s, 1H), 7.83 (s, 1H), 7.68-7.66 (m, 1H), 7.62- 7.60 (m, 2H), 7.52-7.50 (m, 2H), 7.20-7.18 (m, 2H), 6.85 (s, 1H), 5.48 (s, 2H), 5.44-5.42 (m, 1H), 4.55-4.53 (s, 2H), 3.85 (s, 4H), 3.70 (m, 4H), 3.57-3.53 (m, 2H), 0.82-0.78 (m, 2H) and 0.11 (s, 9H). LCMS: [M+H] ⁺ : 596.30, Purity= 94%. Synthesis of 3-formyl-N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)met hyl)-7H-pyrrolo [2,3- d]pyrimidin-6-yl)phenyl)benzenesulfonamide (143):

[00744] To a stirred solution of 3-(hydroxymethyl)-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl (benzene- 1 -sulfonamide 142 (950 mg, 1.59 mmol) in DCM (15 mL) was added MnOz (2.08 g, 23.9 mmol). The resulting reaction mixture was stirred at RT for 5h under N2 atmosphere. After completion of (TLC monitoring), the reaction mixture was filtered through celite bed and washed with DCM (50 mL). The combined organic layer was washed with brine solution (50 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get desired product as brown viscous liquid 143 (720 mg, 76%). The crude was used as such for further next step without purification.

Synthesis of tert-butyl (R)-(l-(3-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)m ethyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)benzyl)piperid in-3-yl)carbamate (144):

[00745] To an ice-cold solution of 3-formyl-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl (benzene- 1 -sulfonamide 143 (756 mg, 1.27 mmol) in DCE (6.0 mL) was added tert-butyl N-[(3R)-piperidin-3-yl]carbamate 18 (510 mg, 2.55 mmol) and sodium acetate (313 mg, 3.82 mmol). The resulting reaction mass was stirred at same temperature for next 30 min, followed by addition of STAB (802 mg, 3.82 mmol) portion wise and stirred the reaction mass at RT for 2h. After completion of reaction (TLC monitoring), the reaction mixture was poured into water (100 mL) and extracted with DCM (2 x 100 mL). The combined organic layer was washed with brine solution, dried over anhydrous NazSCU. filtered and solvent was evaporated under reduced pressure to get the desired product as gummy 144 (600 mg, 37%). LCMS: [M+H] ⁺ : 778.45, Purity= 67%.

Synthesis of (R)-3-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)benzenesulfonamide (145):

[00746] An ice cold solution of tert-butyl N-[(3R)-l-{[3-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl(-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl(sulfamoyl)phenyl]methyl(piperi din-3 -yl]carbamate 144 (600 mg, 771 pmol) in 25% TFA in DCM (8 mL) was stirred at room temperature for Ih. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude was neutralized with aqueous solution of NH4OH and extracted with 20% IPAiCHCh (2 x 100 mL). The combined organic layer was dried over anhydrous NazSCL, filtered and concentrated under reduced pressure to get desired product as brown solid 145 (560 mg, 71%). LCMS: [M+H] ⁺ : (548.17), Purity= 85%.

Synthesis of (R)-N-(l-(3-(N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)sulfamoyl)benzyl)piperidin-3-yl)acrylamide (509):

[00747] To a solution of (R)-3-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)benzenesulfonamide 145 (460 mg, 840 pmol) in THF: water (2:1, 15 mL) was heated at 55°C for 20 min, followed by addition of K3PO4 (178 mg, 840 pmol) portion wise. The resulting reaction mass was stirred at same temperature for 30 min (until clear solution). After completion of starting material, cooled the reaction mass at 10°C, added a solution of 3 -chloropropionyl chloride 122 (40.1 pL, 420 pmol) drop-wise. The resulting reaction mixture was allowed to stirred at 25°C for 2h. After completion of reaction (TLC and LC-MS monitoring), cooled the reaction mass at 0°C, followed by addition of 2M solution of NaOH. The reaction mass was stirred at RT for 16h. The progress on reaction monitored by LC-MS (complete elimination monitored by LCMS). After completion of reaction, the reaction mass was diluted with water (100 mL) and extracted with EtOAc (2 x 100 mL). The combined organic layer was evaporated under reduced pressure to afford the crude product. The crude residue was purified by RP-HPLC purification using 5mM ammonium bicarbonate in water/acetonitrile and column Waters XSelect Phenyl-Hexyl(19*250mm,5pm) to get desired product as white solid 509 (15 mg, 3%).

'H NMR (400 MHz, DMSO-d6)): 8 12.11 (s, 1H), 10.35 (s, 1H), 8.15 (s, 1H), 7.95 (d, J = 7.6 Hz, 1H), 7.75-7.72 (m, 3H), 7.65-7.64 (m, 1H), 7.50-7.48 (m, 2H), 7.12 (d, J= 8.4 Hz, 2H), 7.03 (s, 1H), 6.24-6.17 (m, 1H), 6.09-6.04 (m, 1H), 5.56-5.54 (m, 1H), 3.85-3.83 (m, 4H), 3.78 (m, 1H), 3.73-7.70 (m, 4H), 3.57-3.43 (m, 2H), 2.74-2.72 (m, 1H),2.5O (m, 1H), 1.83-1.78 (m, 2H), 1.68-1.66 (m, 1H), 1.53 (m, 1H), 1.41-1.38 (m, 1H) and 1.12-1.10 (m, 1H). LCMS: [M+H] ⁺ : 602.33, Purity= 99%.

Example B0700-29

Synthesis of Compound B0700-510

[00748] Compound B0700-510 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-N-(l-(4-((N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)sulfamoyl)methyl)phenyl)pyrrolidin-3-yl)acrylamide (510):

Synthesis of l-(4-bromophenyl)-N-(4-(4-morpholino-7-((2-(trimethylsilyl)e thoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)methanesulfonamide (147):

[00749] To a solution of 4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7 H-pyrrolo[2,3- d]pyrimidin-6-yl]aniline 7 (1.0 g, 2.35 mmol) in pyridine (10 mL) was added (4- bromophenyl)methanesulfonyl chloride 146 (1.90 g, 7.05 mmol) and DMAP (144 mg, 1.17 mmol). The resulting reaction mixture was stirred at room temperature for 5h. After completion of reaction (TLC monitoring), solvent was concentrated under reduced pressure. The crude residue was diluted with water (100 mL) and extracted with DCM (2 x 100 mL). The combined organic layer was washed with brine solution, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude was purified by flash chromatography (silca gel, 40 g SNAP) eluted with 35% EtOAc in heptane to get desired product 147 as off-white solid (1.00 g, 68%). LCMS: [M+H] ⁺ : 658.33, Purity= 99%.

Synthesis of N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-p yrrolo [2,3- d]pyrimidin-6-yl)phenyl)-l-(4-(4,4,5,5-tetramethyl-l,3,2-dio xaborolan-2- yl)phenyl)methanesulfonamide (148):

[00750] To a solution of l-(3-bromophenyl)-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}methanesulfonamide 147 (1.0 g, 1.52 mmol) in 1,4-dioxane (20 mL) was added 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl)-l,3,2-dioxaborolane 110 (463 mg, 1.82 mmol) and potassium acetate (223 mg, 2.28 mmol) at room temperature. The resulting reaction mixture was degassed with argon for 15 min, followed by addition of l,r-bis(diphenylphosphino)ferrocene]dichloropalladium(II), DCM complex (124 mg, 152 pmol). The resulting reaction mixture was heated at 100° C for 16h. After the completion of reaction (TLC and LCMS monitoring), the reaction mixture was filtered through celite bed and washed with with ethyl acetate (3 x 50 mL). The combined organic layer washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 2% MeOH in DCM to get the desired product as gummy solid 148 (600 mg, 45%). LCMS: [M+H] ⁺ : 706.41, Purity= 81%.

Synthesis of (4-((N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl) -7H-pyrrolo [2,3- d]pyrimidin-6-yl)phenyl)sulfamoyl)methyl)phenyl)boronic acid (149):

[00751] To a solution of N-{4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl }-7H- pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}-l-[4-(4,4,5,5-tetrameth yl-l,3,2-dioxaborolan-2- yl)phenyl]methanesulfonamide 148 (650 mg, 921 pmol) in acetone (15 mL) was added sodium periodate (985 mg, 4.61 mmol) and ammonium acetate (355 mg, 4.61 mmol) in portion wise. The reaction mixture was stirred at rt for 16h. After completion of reaction (TLC monitoring), solvent was concentrated under reduced pressure. The crude was diluted with water (100 mL) and extracted with 20% IPA: CHCL (3 x 100 mL). The combined organic layer dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get the desired compound 149 as off-white solid (500 mg, 74%). LCMS: [M+H] ⁺ : 624.31, Purity= 86%.

Synthesis of tert-butyl (R)-(l-(4-((N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy) methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)methyl)phenyl) pyrrolidin-3-yl)carbamate (151):

[00752] To a solution of {4-[({4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]met hyl}-7H- pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}sulfamoyl)methyl]phenyl} boronic acid 149 (500 mg, 802 pmol) in ACN (4.00 mL) and ethanol (200 pL) was added tert-butyl N-[(3R)-pyrrolidin-3-yl]carbamate 150 (299 mg, 1.60 mmol) followed by addition of EtsN (300 pL, 1.60 mmol). The resulting reaction mixture was degassed with O2 gas for next 15 minutes and Cu(OAc)2 (146 mg, 802 pmol) was added to the reaction mixture. The resulting reaction mass was heated at 80°C for 4h. After the completion of reaction (TLC and LCMS monitoring), the reaction mixture was filtered through celite bed and washed with ethyl acetate (50 mL). The filtrate was diluted with water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 2% MeOH in DCM to get the desired product as semi solid 151 (130 mg, 16%) . LCMS: [M+H] ⁺ : 764.06, Purity= 72%.

Synthesis of (R)-l-(4-(3-aminopyrrolidin-l-yl)phenyl)-N-(4-(4-morpholino- 7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)methanesulfonamide (152):

[00753] To an ice cold solution of tert-butyl N-[(3R)-l-{4-[({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}sulfamoyl)methyl]phenyl}pyrrolidin-3-yl]carbamate 151 (200 mg, 262 pmol) in DCM (5 mL) was added TFA (2 mL). The resulting reaction mixture was stirred at room temperature for Ih. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude was neutralized with aqueous solution of NH4OH and extracted with 20% IPA:CHC13 (2 x 100 mL). The combined organic layer dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get desired product as brown solid 152 (65 mg, 26%). LCMS: [M+H] ⁺ : (534.22), Purity= 60%.

Synthesis of (R)-N-(l-(4-((N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)sulfamoyl)methyl)phenyl)pyrrolidin-3-yl)acrylamide (510):

[00754] To an ice cold solution of l-{4-[(3R)-3-aminopyrrolidin-l-yl]phenyl}-N-{4-[4-(morpholin - 4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}methanesulfonam ide 152 (120 mg, 225 pmol) in DMF (3.0 mL) was added triethylamine (68.3 mg, 675 pmol) and prop-2-enoyl chloride 27 (22.4 mg, 247 pmol) drop wise. The resulting reaction mixture was stirred at same temperature for 30 min. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was evaporated under reduced pressure. The crude residue was purified through RP-HPLC purification using eluent as 5mM Ammonium Bicarbonate in Water/ Acetonitrile and column using Waters XSelect Phenyl-Hexyl(19*250mm,5pm) to get desired product as white solid 510 (6.0 mg, 4.5%). fiT NMR (400 MHz, DMSO-d6)): 5 12.18 (s, IH), 9.37 (br s, IH), 8.35 (d, J = 7.6 Hz, IH), 8.17 (s, IH), 7.84 (d, J= 8.4 Hz, 2H), 7.21 (d, J= 8.8 Hz, 2H), 7.10 (s, IH), 7.03 (d, J= 7.6 Hz, 2H), 6.48 (d, J= 8.4 Hz, 2H), 6.25-6.18 (m, IH), 6.12-6.07 (m, IH), 5.60-5.55 (m, IH), 4.43-4.40 (m, IH), 4.32 (s, 2H), 3.89-3.87 (m, 4H), 3.76-3.74 (m, 4H), 3.50-3.46 (m, IH), 3.37-3.32 (m, IH), 3.26-3.22 (m, IH), 3.10-3.06 (m, IH), 2.21-2.15 (m, IH) and 1.93-1.88 (m, IH). LCMS: [M+H] ⁺ : 587.95, Purity= 96.44%. Example B0700-30

Synthesis of Compound B0700-511

[00755] Compound B0700-511 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (S)-N-(l-(3-((N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)sulfamoyl)methyl)phenyl)pyrrolidin-3-yl)acrylamide (511):

Synthesis of l-(3-bromophenyl)-N-(4-(4-morpholino-7-((2-(trimethylsilyl)e thoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)methanesulfonamide (154):

[00756] To a solution of 4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7 H- pyrrolo[2,3-d]pyrimidin-6-yl] aniline 7 (15.0 g, 35.2 mmol) in pyridine (30 mL) was added (3- bromophenyl)methanesulfonyl chloride 153 (19.0 g, 70.5 mmol) and DMAP (2.15 g, 17.6 mmol) portion wise. The resulting reaction mixture was stirred at room temperature for 5h. After completion of reaction (TLC monitoring), solvent was concentrated under reduced pressure. The crude residue was diluted with water (100 mL) and extracted with DCM (2 x 100 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get crude. The crude was purified by flash chromatography (silca gel, 40g SNAP) using eluents 35% EtOAc in heptane to get desired compound as brick solid 154 (18.0 g, 64%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 5 12.10 (s, 1H), 8.26 (s, 1H), 7.72 (d, J= 8.0 Hz, 2H), 7.56 (d, J= 8.0 Hz, 2H), 7.47 (s, 1H), 7.34-7.26 (m, 3H), 6.91 (s, 1H), 5.55 (s, 2H), 4.58 (s, 2H), 4.05-3.39 (m, 4H), 3.88-3.73 (m, 4H), 3.64-3.60 (m, 2H), 0.88-0.84 (m, 2H) and 0.079 (s, 9H).

LCMS: [M+H] ⁺ : 658.26, Purity= 88%. Synthesis of te/7-butyl (S)-(l-(3-((N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy) methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)methyl)phenyl) pyrrolidin-3-yl)carbamate (156):

[00757] To a solution of l-(4-bromophenyl)-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl]-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}methanesulfonamide 154 (3.0 g, 4.55 mmol) in 1,4-dioxane (50 mL) was added tert-butyl N-[(3R)-pyrrolidin-3-yl]carbamate 155 (2.83 g, 15.2 mmol) and CS2CO3 (4.45 g, 13.7 mmol). The resulting reaction mixture was degassed with argon for 15 minutes followed by addition of RuPhos (425 mg, 911 pmol) and Pd2(dba)3 (417 mg, 455 pmol) at room temperature. The resulting reaction mixture was stirred at 100°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed and washed with ethyl acetate (50 mL). The filtrate was diluted with water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution, dried over ISfeSCU, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 120 g SNAP) eluted with 2% MeOH in DCM to get desired product as off white solid 156 (2.80 g, 73%).

'H NMR (400 MHz, DMSO-d6): 5 10.04 (s, 1H), 8.27 (s, 1H), 7.72 (d, J = 8 Hz, 2H), 7.32 (d, J = 8 Hz, 2H), 7.19-7.18 (m, 1H), 7.14-7.10 (m, 3H), 6.90 (s, 1H), 6.53-6.46 (m, 2H), 6.32 (s, 1H), 5.55 (s, 2H), 4.42 (s, 2H), 4.08-4.03 (m, 1H), 4.03-4.01 (m, 4H), 3.73-3.70 (m, 4H), 3.62-3.58 (m, 2H), 3.38- 3.37 (m, 1H), 3.28-3.27 (m, 1H), 2.13-2.10 (m, 1H), 1.87-1.85 (m, 1H), 1.3 (s, 9H), 0.087-0.083 (m, 2H) and -0.08 (s, 9H). LCMS: [M+H] ⁺ : 764.44, Purity= 92%.

Synthesis of (S)-l-(3-(3-aminopyrrolidin-l-yl)phenyl)-N-(4-(4-morpholino- 7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)methanesulfonamide (157):

[00758] To an ice cold solution of tert-butyl (S)-(l-(3-((N-(4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)sulfamoyl)methyl)phenyl)pyrrolidin-3-yl)carbamate 156 (6.0 g, 7.85 mmol) in DCM (100 mL) was added TFA (20 mL). The resulting reaction mixture was allowed to stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude residue was neutralized with aqueous NH4OH solution, the solid precipitates was collected by filtration and dried under vaccum to get the desired product as light brown solid 157 (4.0 g, 67%). LCMS: [M+H] ⁺ : 534.30, Purity= 70%. Synthesis of (S)-N-(l-(3-((N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)sulfamoyl)methyl)phenyl)pyrrolidin-3-yl)acrylamide (511):

[00759] A solution of l-{3-[(3S)-3-aminopyrrolidin-l-yl]phenyl}-N-{4-[4-(morpholin -4-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}methanesulfonamide 157 (3.0 g, 5.62 mmol) in THF:H2O (2:1, 45 mL) was heated at 55°C for 20 min (clear solution observed), followed by addition of K3PO4 (2.39 g, 11.2 mmol) portion wise and stirred at same temperature for 30 min (clear solution observed). The resulting reaction mass was cooled at 10°C added solution of 3 -chloropropionyl chloride 122 (856 mg, 1.2 eq., 6.75 mmol) drop-wise. The resulting reaction mixture was stirred at 25°C for 2h. After completion of starting material, cooled the reaction mass and added 2M solution of NaOH. The resulting mixture was stirred at RT for 16h. After completion of reaction, the resulting reaction mass was poured into water (100 mL) and extracted with EtOAc (2 x 100 mL). The combined organic layer evaporated under reduced pressure. The crude residue was purified by column chromatography (silica gel, 100-200M) using eluents 2-2.5% MeOH in DCM to get desired product as light green solid 511 (1.0 g, 30%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 8 12.20 (s, 1H), 9.91 (s, 1H), 8.40 (d, J= 8.0 Hz, 1H), 8.10 (s, 1H), 7.86 (d, J= 8.0 Hz, 2H), 7.25 (d, J= 8.0 Hz, 2H), 7.15-7.11 (m, 2H), 6.53-6.49 (m, 2H), 6.38 (s, 1H), 6.25-6.19 (m, 1H), 6.13-6.09 (m, 1H), 5.60-5.57 (m, 1H), 4.41-4.38 (m, 3H), 3.88-3.83 (m, 4H), 3.74- 7.71 (m, 4H), 3.46-3.42 (m, 1H), 3.21-3.20 (m, 1H), 3.06-3.03 (m, 1H), 3.02-2.98 (m, 1H), 2.21-2.17 (m, 1H) and 1.91-1.88 (m, 1H). LCMS: [M+H] ⁺ : 588.48, Purity= 96.55%.

Example B0700-31

Synthesis of Compound B0700-514

[00760] Compound B0700-514 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of N-((3R)-l-((2-(l-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidi n-6- yl)phenyl)amino)ethyl)pyridin-4-yl)methyl)piperidin-3-yl)acr ylamide (514):

Synthesis of tert-butyl (R)-(l-((2-acetylpyridin-4-yl)methyl)piperidin-3-yl)carbamat e (192): [00761] To an ice-cold solution of tert-butyl N-[(3R)-l-[(2-cyanopyridin-4-yl)methyl]piperidin-3- yl]carbamate 19 (0.5 g, 1.58 mmol) in tetrahydrofuran (10 mL) was added bromo(methyl)magnesium (2.0 M in THF, 3.95 mL, 7.9 mmol). The resulting reaction mixture was stirred at rt for 2h. The progress of reaction monitored by (TLC & LCMS), the resulting reaction mass was quenched with water (20 mL) followed by extraction with EtOAc (3 x 25 mL). The combined organic was washed with brine solution, dried over ISfeSCU, filtered and evaoprated under reduced pressure. The crude residue was purified by column chromatography in silica gel (100- 200M, 12 g, SNAP) eluted with 25-30% ethyl acetate in heptane to get desired product as off white solid 192 (0.4 g, 76%).

¹ H NMR ( 400 MHz, DMSO-d ₆ ): 8 8.66-8.64 (m, 1H), 7.87 (s, 1H), 7.59-7.58 (m, 1H), 6.73-6.71 (m, 1H), 3.58-3.56 (m, 2H), 3.39 (s, 1H), 2.72-2.71 (m, 1H), 2.63 (s, 3H), 2.59-2.56 (m, 1H), 1.89-1.81 (m, 2H), 1.70-1.60 (m, 2H), 1.45-1.42 (m, 1H), 1.39 (s, 9H) and 1.41-1.40 (m, 1H).

LCMS: [M+H] ⁺ : 334.13, Purity= 91%. Synthesis of tert-butyl ((3R)-l-((2-(l-((4-(4-morpholino-7-((2-(trimethylsilyl)ethox y)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)amino)ethyl)pyridin-4-yl )methyl)piperidin-3- yl)carbamate (193):

[00762] To an ice-cold stirred solution of tert-butyl N-[(3R)-l-[(2-acetylpyridin-4- yl)methyl]piperidin-3-yl]carbamate 192 (0.4 g, 1.2 mmol) and 4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 7 (1.02 g, 2.4 mmol) in methanol (5 mL) was added acetic acid (0.14 mL, 2.4 mmol). The resulting reaction mixture was stirred at rt for 30 minute, followed by addition of sodium cyanob or ohydri de (151 mg, 2.4 mmol) portion wise. The resulting reaction mixture was stirred at 80°C for 16h. After completion of reaction (TLC monitoring), the reaction mass was concentrated under reduced preesure. The crude material was poured into ice-cold water (50 mL) and extracted with ethyl acetate (2 x 50 mL) . The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography in silica gel (12 g, SNAP) using eluent 35-40% EtOAc in heptane to get the desired product as light brown solid 193 (0.4 g, 45%). LCMS: [M+H] ⁺ : 743.37, Purity= 79%.

Synthesis of (3R)-l-((2-(l-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6 - yl)phenyl)amino)ethyl)pyridin-4-yl)methyl)piperidin-3-amine (194):

[00763] To an ice-cold solution of tert-butyl N-[(3R)-l-({2-[l-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}amino)ethyl]pyri din-4- yl}methyl)piperidin-3-yl]carbamate 193 (0.3 g, 404 pmol) in dichloromethane (10 mL) was added TFA (2.5 mL). The reaction mixture was stirred at rt for 16h. After completion of reaction (TLC monitoring), solvent was concentrated under reduced pressure to get crude as brown oil 194 (0.3 g TFA salt). The crude used as such for next step.

Synthesis of N-((3R)-l-((2-(l-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidi n-6- yl)phenyl)amino)ethyl)pyridin-4-yl)methyl)piperidin-3-yl)acr ylamide (514):

[00764] To an ice cold stirred solution of (3R)-l-({2-[l-({4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3- d]pyrimidin-6-yl]phenyl}amino)ethyl]pyridin-4-yl}methyl)pipe ridin-3-amine 194 (0.3 g, 585 pmol) in DMF (5.0 mL) was added triethylamine (234 pL, 1.76 mmol) and prop-2-enoyl chloride 27 (47.3 pL, 585 pmol) in drop wise. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC monitoring), the reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude residue was purified by flash column chromatography to get the desired product as white solid 514 (50 mg, 15%).

¹ H NMR ( 400 MHz, DMSO-d ₆ ): 5 11.86 (s, 1H), 8.44-8.43 (m, 1H), 8.10 (s, 1H), 7.92-7.91 (m, 1H), 7.54-7.52 (m, 2H), 7.34-7.33 (m, 1H), 7.17-7.12 (m, 1H), 6.79 (s, 1H), 6.57-6.52 (m, 2H), 6.25-6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.58-5.55 (m, 2H), 4.58-4.54 (m, 1H), 3.81-3.80 (m, 4H) 3.72-3.70 (m, 4H), 3.53-3.47 (m, 1H), 3.41-3.34 (m, 1H), 2.72-2.70 (m, 1H), 2.40-2.38 (m, 1H), 1.92-1.61 (m, 4H), 1.47-1.44 (m, 4H), 1.35-1.33 (m, 1H) and 0.87-0.86 (m, 1H). LCMS: [M+H] ⁺ : 567.17, Purity= 95.11%.

Example B0700-31A

Synthesis of Compound B0700-515

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(3,6-dihy dro-2H-pyran-4- yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (515):

Synthesis of tert-butyl (R)-(l-((2-((4-(4-(3,6-dihydro-2H-pyran-4-yl)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3-yl)carbamate (177):

To a solution of tert-butyl N-[(3R)-l-[(2-{[4-(4-chloro-7-{[2-(trimethylsilyl)ethoxy]met hyl}-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl]carbamoyl}pyridin-4-yl)m ethyl]piperidin-3-yl]carbamate 89 (1.00 g, 1.44 mmol) in 1,4-dioxane (10 mL) and H2O (2.50 mL) was added 2-(3,6-dihydro-2H-pyran- 4-yl)-4,4,5,5-tetramethyl-l,3,2-dioxaborolane 176 (364 mg, 1.73 mmol) and K2CO3 (598 mg, 4.33 mmol) under nitrogen atmosphere. The resulting reaction mixture was degassed with N2 for 15 minutes, followed by addition of PdCh (dtbpf) (94.1 mg, 144 pmol) and reaction mass was heated at 80°C for 16h. After completetion of reaction (TLC monitoring), the resulting reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (3 x 500 mL). The combined organic layer was washed with brine, dried over Na2SO4 , filtered and evaporated under reduced pressure. The crude was was purified by flash column chromatography (silica gel, 120 g SNAP) by using eluent 50% ethyl acetate in heptane to get the desired product as greenish yellow solid 177 (300 mg, 28%). LCMS: [M+H] ⁺ : (740.18), Purity= 94%

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-(3,6-dihydro-2 H-pyran-4-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (178):

To an ice cold stirred solution of tert-butyl N-[(3R)-l-{[2-({4-[4-(3,6-dihydro-2H-pyran-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)pyridin-4- yl]methyl}piperi din-3 -yl]carbamate 177 (250 mg, 338 pmol) in DCM (4.0 mL) was added TFA (2.0 mL). The resulting reaction mixture was stirred at RT for 3h. After completion reaction (TLC and LCMS monitoring), reaction mixture was concentrated under reduced pressure. The crude was triturated with diethyl ether to get desired product as brown solid 178 (170 mg, 88%).

LCMS: [M+H] ⁺ : 510.32, Purity= 90%

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(3,6-dihy dro-2H-pyran-4-yl)- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (515):

To an ice-cold solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-(3,6-dihydro-2 H-pyran-4- yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide 178 (150 mg, 294 pmol) in DMSO (3.0 mL) was added triethylamine (89.4 mg, 883 pmol) and prop-2-enoyl chloride 27 (26 mg, 294 pmol). The resulting reaction mixture was stirred at 0°C for 10 min. After completion of reaction (TLC monitoring). The reaction mixture was quenched with ice cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined layer was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude was purified by RP-HPLC using 5mM ammonium bicarbonate in water/acetonitrile and column waters XBridge C-8(19*250mm,5pm) to get desired product as pale yellow solid 515 (20 mg, 18%).

¹ H NMR (400 MHz, DMSO-t/ ₆ ): 8 12.57 (s, 1H), 10.81 (s, 1H), 8.69 (d, J = 4.8 Hz, 1H), 8.12 (s, 1H), 8.07-7.97 (m, 5H), 7.64 (d, J= 4.4 Hz, 1H), 7.32 (s, 1H), 7.09 (s, 1H), 6.25-6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57 (dd, J= 2.4 & 10.4 Hz, 1H), 4.39-4.38 (m, 2H), 3.90-3.87 (m, 2H), 3.83-3.81 (m, 1H), 3.66 (s, 2H), 2.79-2.74 (m, 3H), 2.66-2.64 (m, 1H), 2.06-2.01 (m, 1H), 1.92-1.87 (m 1H), 1.75-1.68 (m, 2H), 1.58-1.49 (m, 1H) and 1.22-1.10 (m, 2H). LCMS: [M-H] ⁺ : 561.97, Purity= 96.0%. Example B0700-31B

Synthesis of Compound B0700-516

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(tetrahyd ro-2H-pyran-4- yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (516):

Synthesis of tert-butyl (R)-(l-((2-((4-(4-(tetrahydro-2H-pyran-4-yl)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3-yl)carbamate (179):

To a solution of tert-butyl (R)-(l-((2-((4-(4-(3,6-dihydro-2H-pyran-4-yl)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyri din-4- yl)methyl)piperidin-3-yl)carbamate 177 (500 mg, 676 pmol) in propan-2-ol (25.0 mL) and THF (25.0 mL) was added palladium on carbon (50 mg, 10% w/w). The resulting reaction mixture was stirred at room temperature under hydrogen atmosphere at 60 psi for 12h. After completion of reaction (TLC Monitoring), reaction mixture was filtered over celite pad and washed with EtOAc (100 mL). The filtrate was concentrated under reduced pressure and the residue was purified by silica-gel (100-200M) flash column chromatography eluted with 50% EtOAc/Heptane to get desired product as brown solid 179 (380 mg, 33%).

LCMS: [M+H] ⁺ : 742.35, Purity= 60%

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-(tetrahydro-2H -pyran-4-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (180):

To an ice cold solution of tert-butyl (R)-(l-((2-((4-(4-(tetrahydro-2H-pyran-4-yl)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3-yl)carbamate 179 (350 mg, 472 pmol) in DCM (5.0 mL) was added TFA (2.0 mL). The resulting reaction mixture was stirred at room temperature for 3h. After completion reaction (TLC and LCMS monitoring), reaction mixture was concentrated under reduced pressure. The crude was triturated with diethyl ether to get desired product as brown solid 180 (80 mg, 33%).

LCMS: [M+H] ⁺ : 512.25, Purity= 80%.

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(tetrahyd ro-2H-pyran-4-yl)- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (516):

To an ice cold solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-(tetrahydro-2H -pyran-4- yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide 180 (80 mg, 0.156 mmol) in DMF (2 mL) was added EtsN (0.01 mL, 0.469 mmol) and acryloyl chloride 27 (11.0 pL, 0.125 mmol) in drop wise. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC monitoring), the resulting reaction mass was evaporated to dryness and the crude residue was purified through RP-HPLC purification using eluent as 5mM Ammonium Bicarbonate in Water/ Acetonitrile and column using Waters XBridge C-8(19*250mm,5pm) to get desired product as white solid 516 (18 mg, 23%).

'H NMR (400 MHz, DMSO-t/ ₆ : 5 12.49 (s, 1H), 10.81 (s, 1H), 8.69-8.68 (m, 1H), 8.67 (s, 1H), 8.12 (s, 1H), 8.06-8.04 (m, 2H), 8.00-7.96 (m, 3H), 7.64-7.62 (m, 1H), 7.20 (s, 1H), 6.25- 6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57-5.54 (m, 1H), 4.02-3.99 (m, 2H), 3.83-3.81 (m, 1H), 3.66 (s, 2H), 3.57-3.52 (m, 1H), 3.44-3.34 (m, 1H), 2.79-2.78 (m, 1H), 2.67-2.66 (m, 1H), 2.06-1.87 (m, 4H), 1.82-1.78 (m, 4H), 1.58-1.52 (m, 1H) and 1.19-1.17 (m, 2H). LCMS: [M+H] ⁺ : (566.35), Purity= 96.19%

Example B0700-32

Synthesis of Compound B0700-517

[00765] Compound B0700-517 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of N-(4-(4-(3-oxabicyclo[4.1.0]heptan-6-yl)-7H-pyrrolo[2,3-d]py rimidin-6- yl)phenyl)-4-(((R)-3-acrylamidopiperidin-l-yl)methyl)picolin amide (517):-

Synthsis of tert-butyl ((3R)-l-((2-((4-(4-(3-oxabicyclo[4.1.0]heptan-6-yl)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3-yl)carbamate (181):

[00766] To an ice-cold solution of Trimethylsulfoxonium iodide (327 mg, 1.49 mmol) in DMSO (40 mL) was added sodium hydride (60% dispersion in mineral oil, 35.7 mg, 1.49 mmol) portion wise and reaction mixture was stirred at RT for Ih, followed by addition of tert-butyl (R)-(l-((2-((4-(4- (3,6-dihydro-2H-pyran-4-yl)-7-((2-(trimethylsilyl)ethoxy)met hyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyridin-4-yl)methyl)piperidin-3-yl)carba mate 177 (550 mg, 743 pmol). The resulting reaction mixture was stirred at room temperature for Ih and heated at 70°C for 3h. After the completion of reaction (LCMS monitoring), the reaction mixture was poured into ice cold water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was dried over sodium sulphate, filtered and concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 120 g SNAP) eluted wuth 1-2% MeOH in DCM to get the desired product as brown gummy solid 181 (300 mg, 49%)

^X H NMR (400 MHz, DMSO-d6): 5 10.87 (s, IH), 8.81-8.77 (m, 2H), 8.12-8.10 (m, 3H), 7.87-7.84 (m, 2H), 7.63-7.62 (m, IH), 6.91 (s, IH), 6.76-6.74 (m, IH), 5.67 (s, 2H), 3.97-3.92 (m, 3H), 3.65- 3.61 (m, 4H), 3.43-3.41 (m, IH), 2.66-2.61 (m, 2H), 2.40-2.35 (m, IH), 2.12-2.04 (m, 4H), 1.98-1.60 (m, 4H), 1.35 (s, 9H), 1.23-1.19 (m, 1H), 1.15-1.10 (m, 2H) 0.88-0.84 (m, 2H), and 0.08 (s, 9H). LCMS: [M+H+: (754.51), Purity=92%.

Synthsis of N-(4-(4-(3-oxabicyclo[4.1.0]heptan-6-yl)-7H-pyrrolo[2,3-d]py rimidin-6-yl)phenyl)- 4-(((R)-3-aminopiperidin-l-yl)methyl)picolinamide (182):

[00767] To an ice cold solution of tert-butyl N-[(3R)-l-[(2-{[4-(4-{3-oxabicyclo[4.1.0]heptan-6- yl}-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]py rimidin-6- yl)phenyl]carbamoyl}pyridin-4-yl)methyl]piperidin-3-yl]carba mate 181 (800 mg, 1.06 mmol) in DCM (16 mL) was added TFA (4 mL) in drop wise manner. The resulting reaction mixture was allowed to stir at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude residue was neutralized with aqueous NH4OH solution to get solid precipitates, filtered and dried under vaccum to get the desired product as pale yellow solid 182 (140 mg, 25%).

¹ H NMR (400 MHz, DMSO-d6): 8 10.81 (s, 1H), 8.69-8.68 (m, 1H), 8.58 (s, 1H), 8.12 (s, 1H), 8.06- 8.04 (m, 2H), 8.01-7.99 (m, 2H), 7.61-7.60 (m, 2H), 7.09 (s, 1H), 3.98-3.93 (m, 2H), 3.67-3.59 (m, 3H), 3.46-3.43 (m, 2H), 2.70-2.62 (m, 4H), 2.29-2.28 (m, 3H), 1.98-1.95 (m, 2H), 1.74 (m, 2H), 1.62 (m, 1H), 1.47-1.45 (m, 2H) and 1.10 (m, 1H). LCMS: [M+H] ⁺ : 524.23, Purity= 92%.

Synthesis of N-(4-(4-(3-oxabicyclo[4.1.0]heptan-6-yl)-7H-pyrrolo[2,3-d]py rimidin-6-yl)phenyl)- 4-(((R)-3-acrylamidopiperidin-l-yl)methyl)picolinamide (517):

[00768] To an ice cold solution of N-(4-(4-(3-oxabicyclo[4.1.0]heptan-6-yl)-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)-4-(((R)-3-aminopiperidin-l-yl)methy l)picolinamide 182 (140 mg, 0.267 mmol) in DMF (5 mL) was added EtsN (0.11 mL, 0.267 mmol) and acryloyl chloride 27 (24 mg, 0.267 mmol) in drop wise. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC monitoring), the resulting reaction mass was evaporated under reduced pressure. The crude residue was purified by flash chromatography in silca gel (silica gel, 12g, SNAP) eluted with 2-3% MeOH in DCM to get desired product as light yellow solid 517 (40 mg, 25%).

^X H NMR (400 MHz, DMSO-d6): 8 12.50 (s, 1H), 10.80 (s, 1H), 8.69-8.68 (m, 1H), 8.58 (s, 1H), 8.12 (s, 1H), 8.06-8.04 (m, 2H), 8.04-7.97 (m, 3H), 7.64-7.62 (m, 1H), 7.09 (s, 1H), 6.25-6.19 (m, 1H), 6.07-6.02 (m, 1H), 5.57-5.54 (m, 1H), 3.98-3.97 (m, 1H), 3.94 (s, 1H), 3.82-3.81 (m, 1H), 3.66 (m, 3H), 3.47-3.42 (m, 1H), 2.79-2.77 (m, 1H), 2.67-2.62 (m, 2H), 2.32-2.23 (m, 1H), 2.06-1.88 (m, 3H), 1.78-1.69 (m, 2H), 1.58-1.49 (m, 1H), 1.45-1.45 (m, 1H), 1.26-1.23 (m, 1H), and 1.09-1.08 (m, 1H). LCMS: [M+H] ⁺ : (578.42), Purity= 97.16%. Example B0700-35

Synthesis of Compound B0700-524

[00769] Compound B0700-524 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R,E)-4-((3-(4-(cyclopropyl(methyl)amino)but-2-enamido)piper idin-l- yl)methyl)-N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-y l)phenyl)picolinamide (524):

Synthesis of tert-butyl (E)-4-(cyclopropyl(methyl)amino)but-2-enoate (190):

[00770] To an ice-cold solution of N-methylcyclopropanamine 189 (354 mg, 4.98 mmol) in THF (15.0 mL) were added triethylamine (1.24 mL, 9.05 mmol) and tert-butyl (2E)-4-bromobut-2-enoate 13 (1.0 g, 4.52 mmol). The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) using eluents 10% EtOAc in heptane to get brown solid 190 (230 mg, 24%).

^X HNMR (400 MHz, CDCh): 5 6.90-6.83 (m, 1H), 5.89 (d, J= 15.6 Hz, 1H), 3.30 (d, J = 6.4 Hz, 1H), 2.75 (s, 3H), 2.34 (s, 1H), 1.73 (m, 1H), 1.47 (s, 9H), 0.49-0.47 (m, 4H). LCMS: [M+H] ⁺ : 212.21, Purity= 99%.

Synthesis of (E)-4-(cyclopropyl(methyl)amino)but-2-enoic acid (191):

[00771] An ice cold solution of tert-butyl (2E)-4-[cyclopropyl(methyl)amino]but-2-enoate 190 (230 mg, 1.09 mmol) in 6N-HC1 (5.00 mL) was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure and azeotroped with toluene (10 mL) to get desired product 191 as white solid (180 mg, quantitative).

^X H NMR (400 MHz, MeOD): 8 6.96-6.66 (m, 1H), 6.31 (d, J = 15.2 Hz, 1H), 4.11 (d, J =7.6 Hz, 2H), 2.97 (s, 3H), 2.90-2.87 (m, 1H) and 1.02-1.01 (m, 4H). MS= [M+H]+: (156.08)

Synthesis of (R,E)-4-((3-(4-(cyclopropyl(methyl)amino)but-2-enamido)piper idin-l-yl)methyl)- N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)pi colinamide (524):

[00772] To an ice cold solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide 10 (450 mg, 878 pmol) in N,N- dimethylformamide (10.0 mL) was added (2E)-4-[cyclopropyl(methyl)amino]but-2-enoic acid 191 (163 mg, 1.2 eq., 1.05 mmol), DIPEA (767 pL, 5 eq., 4.39 mmol) and T3P (50% in ethyl acetate, 0.9 mL, 2.63 mmol). The resulting reaction mixture was stirred at room temperature for 2h. After completion of reaction (TLC monitoring), solvent was evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) using 2-3% MeOH in DCM as eluent to get the desired product as white solid 524 (60 mg, 11%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 5 12.2 (s, 1H), 10.74 (s, 1H), 8.68 (d, J= 4.4 Hz, 1H), 8.31 (s, 1H), 8.18 (s, 1H), 8.01-7.99 (m, 2H), 7.92-7.90 (m, 3H), 7.62 (d, J= 4.0 Hz, 1H), 7.16 (s, 1H), 6.58- 6.52 (m, 1H), 6.02-5.98 (m, 1H), 3.89-3.88 (m, 4H), 3.81-3.74 (m, 5H), 3.65 (s, 1H), 3.19 (m, 2H), 3.12- 3.05 (m, 2H), 2.77-2.75 (m, 1H), 2.66-2.63 (m, 1H), 2.20 (s, 3H), 2.03 (m, 1H), 1.90-1.88 (m, 1H), 1.76-1.51 (m, 1H), 1.23-1.15 (m, 4H) 0.41 (s, 2H) and 0.28 (s, 1H).

LCMS: [M+H] ⁺ : 650.49, Purity= 98%.

Example B0700-36

Synthesis of Compound B0700-526

[00773] Compound B0700-526 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-morpholin o-7H- pyrrolo [2, 3-d] pyrimidin-6-yl)cyclohexyl)picolinamide (526) :

Synthesis of tert-butyl (4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyr rolo[2,3- d]pyrimidin-6-yl)cyclohex-3-en-l-yl)carbamate (196):

[00774] To a stirred solution of 4-(6-iodo-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2, 3- d]pyrimidin-4-yl)morpholine 5 (1.00 g, 2.17 mmol) and tert-butyl N-[4-(4,4,5,5-tetramethyl-l,3,2- dioxaborolan-2-yl)cyclohex-3-en-l-yl] carbamate 195 (843 mg, 2.61 mmol) in 1,4-dioxane (12.0 mL) was added K2CO3 (901 mg, 6.52 mmol) in water (8.0 mL) at room temperature under nitrogen atmosphere. The resulting reaction mixture was degassed with nitrogen for 10 min, followed by addition of PdC12(dppf) (141 mg, 217 pmol) and heated at 100°C for 16h . After completion of reaction (TLC monitoring) the reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (3 x 250 mL). The combined organic layer was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 12g SNAP) by using eluent 20% ethyl acetate in heptane to get the desired product as white solid 196 (950 mg, 78%).

¹ H NMR ( 400 MHz, DMSO-de): 5 8.21 (s, 1H), 6.87-6.85 (m, 1H), 6.69 (s, 1H), 6.18 (s, 1H), 5.54 (s, 2H), 3.83-3.82 (m, 4H), 3.72-3.71 (m, 4H), 3.62-3.58 (m, 2H), 2.39 (m, 2H), 2.13-2.10 (m, 2H), 1.90-1.87 (m, 2H), 1.60-1.58 (m, 1H), 1.39 (s, 9H), 0.87-0.83 (m, 2H) and -0.07 (s, 9H).

LCMS: [M+H] ⁺ : 530.37, Purity= 96%. Synthesis of tert-butyl (4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyr rolo[2,3- d]pyrimidin-6-yl)cyclohexyl)carbamate (197):

[00775] To a stirred solution of tert-butyl N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl(-7H-pyrrolo[2, 3-d]pyrimidin-6-yl]cy cl ohex-3-en-l-yl (carbamate 196 (500 mg, 944 pmol) in methanol (10.0 mL) was added Pd/C (50 mg, 10% w/w) and the resulting reaction mixture was stirred at room temperature under H2 atmosphere. After completion of reaction (LCMS monitoring) the reaction mixture was passed through celite bed and washed with methanol. The filtrate was concentrated under reduced pressure to get desired product as viscous liquid 197 (480 mg, 96%). LCMS: [M+H] ⁺ : 532.39, Purity= 98%.

Synthesis of 4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyrr olo[2,3-d]pyrimidin- 6-yl)cyclohexan-l-amine (198):

[00776] To an ice-cold solution of tert-butyl N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl(-7H-pyrrolo[2,3-d]pyrimidin-6- yl]cyclohexyl (carbamate 197 (480 mg, 903 pmol) in DCM (10.0 mL) was added p-TSA (687 mg, 3.61 mmol). The resulting reaction mixture was stirred at RT for 16h under N2 atmosphere. After completion of reaction (TLC monitoring), the reaction mixture was basified with saturated solution of NaHCOs and extracted with DCM (2 x 100 mL). The combined organic layer was washed with brine solution, dried over anhydrous ISfeSCL, filtered and concentrated under reduced pressure to get brown viscous 198 (360 mg, 92%).

'H NMR (400 MHz, DMSO-d ₆ ): 5 8.19 (s, 1H), 6.52-6.48 (m, 1H), 5.56 (s, 2H), 3.79 (s, 4H), 3.73- 7.71 (m, 4H), 3.49-3.45 (m, 2H), 2.80-2.74 (m, 2H), 1.98-1.94 (m, 2H), 1.88-1.86 (m, 3H), 1.68-1.64 (m, 2H), 1.62 (m, 2H), 1.23-1.20 (m, 1H), 0.85-0.82 (m, 2H) and -0.093 (s, 9H).

LCMS: [M+H] ⁺ : 432.24, Purity= 95%.

Synthesis of tert-butyl (R)-(l-((2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)me thyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)cyclohexyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3- yl)carbamate (199):

[00777] To an ice-cold solution of 4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl(-7 H- pyrrolo[2,3-d]pyrimidin-6-yl]cyclohexan-l -amine 198 (360 mg, 834 pmol) in N,N- dimethylformamide (5.0 mL) was added 4-{[(3R)-3-{[(tert-butoxy)carbonyl]amino(piperidin-l- yl]methyl(pyridine-2-carboxylic acid 8 (336 mg, 1.00 mmol), HATU (476 mg, 1.25 mmol) and DIPEA (431 pL, 2.50 mmol). The resulting reaction mixture was stirred at RT for Ih. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice-cold water (100 mL). The resultant solid was filtered and dried under high vacuum to get desired product as brown solid 199 (400 mg, 64%). LCMS: [M+H] ⁺ : 749.56, Purity= 80%.

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3- d]pyrimidin-6-yl)cyclohexyl)picolinamide (200):

[00778] To an ice-cold stirred solution of tert-butyl N-[(3R)-l-{[2-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]cy cl ohexyl} carbarn oyl)pyri din-4- yl]methyl}piperi din-3 -yl]carbamate 199 (400 mg, 534 pmol) in DCM (10 mL) was added TFA (5.00 mL) dropwise. The resulting reaction mixture was stirred at RT for 3h. After completion of starting material (LCMS monitoring), the reaction mixture was concentrated under reduced pressure. The crude was dissolved in 1,4-dioxane (5.00 mL) added ethane- 1,2-diamine (80.2 mg, 1.34 mmol). The reaction mixture was stirred at 80°C for 30 min and the reaction mixture was concentrated under reduced pressure. The crude was diluted with water (50 mL) and extracted with 25% nhACHCL (3 X 100 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get desired product as brown solid 200 (200 mg, 72%).

LCMS: [M+H] ⁺ : 519.34, Purity= 70%.

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-morpholin o-7H-pyrrolo[2,3- d]pyrimidin-6-yl)cyclohexyl)picolinamide (526):

[00779] To an ice-cold stirred solution of 4-{[(3R)-3-aminopiperidin-l-yl]methyl}-N-{4-[4- (morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]cycl ohexyl }pyridine-2-carboxami de 200 (200 mg, 386 pmol) in N,N-dimethylformamide (3.00 mL) was added EtsN (160 pL, 1.16 mmol) and prop-2-enoyl chloride 27 (34.9 mg, 386 pmol). The resulting reaction mixture was stirred for next 15 min at 0°C. After completion of reaction (TLC & LCMS monitoring) the reaction mixture was poured into ice-cold water (100 mL) and extracted with 25% DLACHCL (2x 100 mL). The organic layer was washed with brine solution, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude was purified through RP-HPLC purification to get desired product as white solid 526 (16 mg, 7.25%).

'H NMR (400 MHz, DMSO-d ₆ @HT): 5 11.61 (br s, 1H), 8.56 (d, J= 4.8 Hz, 1H), 8.30 (d, J= 8.0 Hz, 1H), 8.11 (s, 1H), 7.99 (s, 2H), 7.69 (d, J= 7.6 Hz, 1H), 7.52 (d, J= 3.6 Hz, 1H), 6.34-6.31 (m, 1H), 6.24-6.19 (m, 1H), 6.07-6.02 (m, 1H), 5.54 (d, J= 10.0 Hz, 1H), 3.81-3.80 (m, 6H), 3.77-3.74 (s, 4H), 3.61 (s, 2H), 2.77-2.75 (m, 1H), 2.63-2.61 (m, 1H), 2.10-2.07 (m, 2H), 1.99-1.96 (m, 2H), 1.84- 1.77 (m, 2H), 1.67-1.61 (m, 3H) and 1.57-1.55 (m, 5H). LCMS: [M+H] ⁺ : 573.62, Purity= 92% Example B0700-35

Synthesis of Compound B0700-529

[00780] Compound B0700-529 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(3-fluoro-4-(4- morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (529):

Synthesis of 3-fluoro-4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl )-7H-pyrrolo [2,3- d]pyrimidin-6-yl)aniline (202):

[00781] To a stirred solution of 4-(6-iodo-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2, 3- d]pyrimidin-4-yl)morpholine 6 (500 mg, 1.09 mmol) in 1,4-dioxane: acetonitrile (1 : 1, 6.0 mL) was added 4-fluoro-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyri din-2-amine 201 (282 mg, 1.18 mmol) and aqueous solution of K3PO4 (461 mg, 2.17 mmol) in water (2.5 mL). The resulting reaction mixture was degassed with argon gas for 10 min, followed by addition of bis(4-(di-/c/7- butylphosphanyl)-N,N-dimethylaniline); dichloropalladium (61.5 mg, 86.9 pmol). The resulting reaction mixture was stirred at 90°C for 3h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (50 mL) and extracted with EtOAc (3 x 50 mL). The combined organic layer was washed with brine, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude was purified through flash column chromatography (silica gel, 12g SNAP) eluted with 15% EtOAc:Heptane to get desired product 202 as light brown solid (300 mg, 62%). LCMS: [M+H] ⁺ : 444.21, Purity= 92%. Synthesis of tert-butyl (R)-(l-((2-((3-fluoro-4-(4-morpholino-7-((2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)carbamoyl)pyridin-4- yl)methyl)piperidin-3-yl)carbamate (203):

[00782] To an ice-cold stirred solution of 3-fluoro-4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 202 (300 mg, 2.98 mmol) and 4-{[(3R)-3-{[(tert-butoxy)carbonyl]amino}piperidin-l-yl]meth yl}pyridine-2-carboxylic acid 8 (227 mg, 2.98 mmol) in acetonitrile (5.0 mL) was added 1 -methyl- IH-imidazole (166 mg, 8.94 mmol) and [chloro(dimethylamino)methylidene]dimethylazanium; hexafluoro-V-phosphanuide (378 mg, 5.96 mmol). The resulting reaction mixture was stirred at RT for 16h . After completion of reaction (TLC monitoring ), the reaction mixture was diluted with water (25 mL) and extracted with DCM (3 x 25 mL). The combined organic layer was washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude was purified through flash column chromatography (silica gel, 12 g SNAP) eluted with 20% EtOAc:Heptane to get desired product as brown viscous liquid 203 (350 mg, ).

LCMS: [M+H] ⁺ : 761.55, Purity=80%

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(3-fluoro-4-(4-morph olino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (204):

[00783] To an ice-cold stirred solution of tert-butyl N-[(3R)-l-{[2-({3-fhioro-4-[4-(morpholin-4-yl)- 7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimi din-6-yl]phenyl}carbamoyl)pyri din-4- yl]methyl}piperi din-3 -yl]carbamate 203 (350 mg, 131 pmol) in dichloromethane (6.0 mL) was added trifluoroacetic acid (6.0 mL) dropwise. The resulting reaction mixture was stirred at RT for 3h. After completion of reaction ( TLC and LCMS monitoring), the reaction mixture was concentrated under reduced pressure. The crude was triturated with pentane to get the desired product as brown solid 204 (200 mg, crude as TFA salt). LCMS: [M+H] ⁺ : 531.39.

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(3-fluoro-4-(4- morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (529):

[00784] To an ice-cold stirred solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(3-fluoro-4-(4- morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinami de 204 (200 mg, 94.2 pmol) in N,N- dimethylformamide (2.0 mL) was added tri ethylamine (156 pL, 848 pmol) and prop-2-enoyl chloride 27 (34 mg, 283 pmol). The reaction mixture was stirred at same temperature for 15 minute. After completion of reaction (TLC and LCMS monitoring), the reaction mixture was poured into ice-cold water (50 mL) and extracted with DCM (2 x 50 mL). The combined organic layer was washed with brine solution, dried over anhydrous NaiSCh, filtered and concentrated under reduced pressure. The crude was purified through flash column chromatography ( silica gel, 12 g SNAP) eluted with 1 % MeOH:DCM to get the desired product as white solid 529 (19 mg, 34%).

NMR (400 MHz, DMSO-d ₆ ): 6 12.18 (s, 1H), 10.99 (s, 1H), 8.70-8.69 (m, 1H), 8.21 (s, 1H), 8.11 (s, 1H), 8.08-8.04 (m, 1H), 8.00-7.98 (m, 2H), 7.91-7.88 (m, 2H), 7.64-7.63 (m, 1H), 7.00 (s, 1H), 6.25-6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57-5.54 (m, 1H), 3.89-3.88 (m, 4H), 3.83-3.81 (m, 1H), 3.76- 3.73 (m, 4H), 3.66 (s, 1H), 2.79-2.77 (m, 1H), 2.66-2.64 (m, 1H), 2.06-2.03 (m, 1H), 1.92-1.87 (m, 1H), 1.77-1.68 (m, 2H), 1.55-1.50 (m, 1H) and 1.19-1.17 (m, 1H). LCMS: [M+H] ⁺ : 585.55 , Purity = 98.62%.

Example B0700-38

Synthesis of Compound B0700-535

[00785] Compound B0700-535 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-N-(l-(3-((N-(-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6 - yl)phenyl)sulfamoyl)methyl)phenyl)pyrrolidin-3-yl)acrylamide (535):

Synthesis of tert-butyl (R)-(l-(3-((N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy) methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)methyl)phenyl) pyrrolidin-3-yl)carbamate (206):

[00786] To a stirred solution of l-(4-bromophenyl)-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}methanesulfonamide 154 (0.5 g, 0.75 mmol) in toluene (5 mL) was added tert-butyl (R)-pyrrolidin-3-ylcarbamate 205 (0.28

- 249 -

RECTIFIED SHEET (RULE 91 ) ISA/EP g, 1.51 mmol) and CS2CO3 (0.74 g, 2.27 mmol). The resulting reaction mixture was degassed with nitrogen for 15 minutes followed by addition of RuPhos (70 mg, 0.15 mmol) and Pd2(dba)3 (70 mg, 0.075 mmol). The resulting reaction mixture was stirred at 100°C for 16h under nitrogen atmosphere. After completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed and washed with ethyl acetate (2x 50 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 12 g SNAP) eluted with 45% EtOA in heptane to get desired product 206 as off white solid (0.55 g, 94%).

LCMS: [M+H] ⁺ : 764.45, Purity= 85%.

Synthesis of (R)-l-(3-(3-aminopyrrolidin-l-yl)phenyl)-N-(4-(4-morpholino- 7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)methanesulfonamide (207):

[00787] To an ice cold solution of tert-butyl (R)-(l-(3-((N-(4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)sulfamoyl)methyl)phenyl)pyrrolidin-3-yl)carbamate 206 (0.45 g, 0.58 mmol) in DCM (6 mL) was added TFA (3 mL). The resulting reaction mixture was stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude was dissolved in 1,4-dioxane (5.0 mL) was added ethane-l,2-diamine (88 mg, 1.47 mmol) and reaction mixture was refluxed for 2h. The reaction mixture was concentrated under reduced pressure. The crude was diluted with water (50 mL) and extracted with 20% IPA CHCL (2 x 50 mL). The combined organic layer was dried over Na2SO4, filtered and concentrated under reduced pressureto get desired compound as yellow solid 207 (0.35 g, 92%). LCMS: [M+H] ⁺ : 534.29, Purity= 82%.

Synthesis of (R)-N-(l-(3-((N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)sulfamoyl)methyl)phenyl)pyrrolidin-3-yl)acrylamide (535):

[00788] A solution of l-{3-[(3R)-3-aminopyrrolidin-l-yl]phenyl}-N-{4-[4-(morpholin -4-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}methanesulfonamide 207 (200 mg, 375 pmol) in THFiLEO (2:1, 6.0 mL) was heated at 55°C for 20 minute (clear solution observed), followed by addition of K3PO4 (159 mg, 750 pmol) portion wise and stirred at same temperature for next 30 min (until clear solution). The resulting reaction mass was cooled at 10°C, added 3 -chloropropionyl chloride 122 (57 mg, 450 pmol) in drop-wise. The resulting reaction mixture was allowed to stirred at rt for 2h. After completion of starting material (TLC monitoring), cooled to 0°C added 2M solution of NaOH and stirred at RT for 16h. The progress of reaction (complete elimination) monitored by LCMS. After completion of reaction, the resulting reaction mass was poured into water (100 mL) and extracted with EtOAc (2 x 100 mL). The combined organic layer was dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by combi flash (silica gel, 12g SNAP) using 2-3% MeOH in DCM to get desired product as white solid 535 (40 mg, 18%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 12.20 (br s, 1H), 9.90 (s, 1H), 8.39 (d, J = 8.0 Hz, 1H), 8.18 (s, 1H), 7.86 (d, J = 4.0 Hz, 2H), 7.25 (d, J = 8.0 Hz, 1H), 7.15 (d, J = 8.0 Hz, 1H), 6.53-6.49 (m, 2H), 6.39 (s, 1H), 6.26-6.19 (m, 1H), 6.13-6.09 (m, 1H), 5.60-5.57 (m, 1H), 4.43-4.41 (m, 1H), 4.38 (s, 2H), 3.89-3.87 (m, 4H), 3.76-3.73 (m, 4H), 3.46-3.42 (m, 2H), 3.32 (m, 2H), 3.21-3.20 (m, 1H), 3.06- 3.03 (m, 1H), 2.21-2.17 (m, 1H) and 1.92-1.88 (m, 1H). LCMS: [M+H] ⁺ : 588.51, Purity= 97%.

Example B0700-39

Synthesis of Compound B0700-542

[00789] Compound B0700-542 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-N-(l-(3-((N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)sulfamoyl)methyl)phenyl)piperidin-3-yl)ethenesulfo namide (542):

Synthesis of tert-butyl (R)-(l-(3-((N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy) methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)methyl)phenyl) piperidin-3-yl)carbamate (208):

[00790] To a solution of l-(3-bromophenyl)-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}methanesulfonamide 154 (500 mg, 759 pmol) and tert-butyl (R)-piperi din-3 -ylcarbamate 18 (532 mg, 2.66 mmol) in toluene (10.0 mL) was added CS2CO3 (742 mg, 2.28 mmol). The reaction mixture was degassed with nitrogen for 10 min, followed by addition of [2',6'-bis(propan-2-yloxy)-[l,l'-biphenyl]-2- yl]dicyclohexylphosphane (106 mg, 0.3 eq., 228 pmol) and tris((lE,4E)-l,5-diphenylpenta-l,4-dien- 3-one) palladium (92.1 mg, 114 pmol). The resulting reaction mixture was stirred at 100°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer washed with brine solution, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude was purified by flash column chromatography ( silica gel, 12 g SNAP) eluted with 37% EtOAc:Heptane to get desired product as light brown solid 208 (340 mg, 38.57%). LCMS: [M+H] ⁺ : 778.52, Purity=89%.

Synthesis of (R)-l-(3-(3-aminopiperidin-l-yl)phenyl)-N-(4-(4-morpholino-7 H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)methanesulfonamide (209):

[00791] To an ice-cold solution of tert-butyl N-[(3R)-l-{3-[({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}sulfamoyl)methyl]phenyl}piperidin-3-yl]carbamate 208 (340 mg, 437 pmol) in dichloromethane (6.0 mL) was added trifluoroacetic acid (2.0 mL) dropwise . The reaction mixture was stiired at RT for 3h. After completion of reaction (LCMS monitoring), the reaction mixture was concentrated under reduced pressure. The crude was dissolved in 1,4-dioxane (5.00 mL) added ethane- 1,2-diamine (65.7 mg, 1.09 mmol). The resulting reaction mixture was stirred at 80°C for Ih. After completion of reaction (LCMS monitoring) the reaction mixture was concentrated under reduced pressure. The crude was diluted with water (50.0 mL) and extracted with 25% IPA:CHC13 (2 x 50 mL). The combined organic layer was washed with brine solution (50 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get brown viscous mass 209 (240 mg, 82%). LCMS: [M+H] ⁺ : 548.35, Purity= 82%.

Synthesis of (R)-N-(l-(3-((N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)sulfamoyl)methyl)phenyl)piperidin-3-yl)ethenesulfo namide (542):

[00792] To a stirred solution of l-{3-[(3R)-3-aminopiperidin-l-yl]phenyl}-N-{4-[4-(morpholin- 4- yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}methanesulfonamid e 209 (230 mg, 420 pmol) in DMF (2.50 mL) was added triethylamine (15.6 pL, 112 pmol) and ethenesulfonyl chloride 160 (5.69 mg, 45.0 pmol) dropwise. The resulting reaction mixture was stirred at RT for 15 min . After completion of reaction (TLC and LCMS monitoring) the reaction mixture was poured into ice-cold water (20.0 mL) and extracted with 10% DLACHCL (2 x 50 mL). The combined orgainc layer was washed with brine, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude was purified through RP-HPLC purification to get 542 (16 mg, 5.79%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 5 12.30 (br s, 1H), 9.95 (s, 1H), 8.20 (s, 1H), 7.88-7.85 (m, 2H), 7.53-7.51 (m, 1H), 7.24-7.22 (m, 2H), 7.20-7.18 (m, 1H), 6.88-6.86 (m, 1H), 6.81-6.74 (m 2H), 6.68-

6.66 (m, 1H), 6.08-6.03 (m, 1H), 5.95-5.93 (m, 1H), 4.42 (s, 2H), 3.90-3.88 (m, 4H), 3.76-3.74 (m, 4H), 3.61-3.59 (m, 1H), 3.43 (m, 2H), 3.15-3.13 (m, 1H), 2.58-2.56 (m, 2H), 1.88-1.84 (m, 1H), 1.72-

1.67 (m, 1H), 1.48-1.45 (m, 1H) and 1.34-1.30 (m, 1H). LCMS: [M+H] ⁺ : 638.48, Purity= 97.08%.

Example B0700-42

Synthesis of Compound B0700-557

[00793] Compound B0700-557 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-4-((3-(acrylamido-2,3,3-d3)piperidin-l-yl)methyl)-N-(4-( 4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (557):

Synthesis of (R)-4-((3-(acrylamido-2,3,3-d3)piperidin-l-yl)methyl)-N-(4-( 4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (557):

[00794] To a stirred solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide 10 (200 mg, 0.39 mmol) and acrylic-2, 3, 3-d3 acid 222 (30 mg, 0.39 mmol) in N,N-dimethylformamide (3.0 mL) was added DIPEA (0.35 mL, 1.95 m mol) and T3P (50% in EtOAc, 0.74 mL , 1.17 mol) at same temperature. The reaction mixture was stirred at RT for 3h. After the completion of reaction (TLC monitoring) reaction mixture was diluted with ice cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was dried over Na2SO4, filtered and evaporated under reduced pressure. The crude residue was purified by RP-HPLC purification to get yellow solid 557 (19 mg, 8.5%). ¹ H NMR (400 MHz, DMSO-t/e): 012.21 (s, 1H), 10.75 (s, 1H), 8.69 (d, J = 5.2 Hz, 1H), 8.18 (s, 1H), 8.11 (s, 1H), 8.01-7.99 (m, 3H), 7.92 (d, J = 8.8 Hz, 2H), 7.63 (d, J = 4.4 Hz, 1H), 7.16 (s, 1H), 3.88- 3.87 (m, 4H), 3.82-3.81 (m, 1H), 3.76-3.75 (m, 4H), 3.65 (s, 2H), 2.79-2.77 (m, 1H), 2.65 (m, 1H), 2.06-2.03 (m, 1H), 1.92-1.87 (m, 1H), 1.78-1.68 (m, 2H), 1.54-1.52 (m, 1H) and 1.22-1.19 (m, 1H). LCMS: [M+H] ⁺ : 570.59, Purity= 98.80%.

Example B0700-44

Synthesis of Compound B0700-559

[00795] Compound B0700-559 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of N-((3R)-l-(3-(l-(N-(2-fluoro-4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)sulfamoyl)ethyl)phenyl)piperidin-3-yl)acrylamide (559):

Synthesis of 2-fluoro-4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl )-7H-pyrrolo [2,3- d]pyrimidin-6-yl)aniline (229):

[00796] To a stirred solution of 4-(6-iodo-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2, 3- d]pyrimidin-4-yl)morpholine 5 (2.0 g, 4.34 mmol) in 1,4-dioxane (21 mL) was added 2-fluoro-4- (4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)aniline 228 (1.03 g, 4.34 mmol) and CS2CO3 (1.38 g, 13.0 mmol) in water (9.0 mL). The resulting reaction mixture was degassed with nitrogen for 15 min, followed by addition of Pd(PPh3)4 (502 mg, 434 pmol) and the resulting reaction mixture was stirred at 100°C for 16h under nitrogen atmosphere. After the completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed and washed with ethyl acetate (100 mL). The organic layer was diluted with water (50 mL) and extracted with ethyl acetate (2 x 50 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 30% ethyl acetate in heptane to get the desired product as brown solid 229 (1.70 g, 88%). LCMS: [M+H] ⁺ : 444.29, Purity= 93%.

Synthesis of l-(3-bromophenyl)-N-(2-fluoro-4-(4-morpholino-7-((2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)methanesulfonamide (230):

[00797] To a stirred solution of 2-fluoro-4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy] methyl}- 7H-pyrrolo[2,3-d]pyrimidin-6-yl]aniline 229 (1.60 g, 3.61 mmol) in dichloromethane (32.0 mL) was added DIPEA (3.11 mL, 18.0 mmol) and DMAP (44.1 mg, 361 pmol). The resulting reaction mixture was stirred for 20 min at rt, followed by addition of (3-bromophenyl)methanesulfonyl chloride 153 (2.92 g, 10.8 mmol) portion wise. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (50 mL) and extracted with DCM (2 x 100 mL). The combined combined organic layer was dried over Na2SC>4, filtered and concentrated under reduced pressure. The crude residue was purified by column chromatography in silica gel (100-200M) eluted with 20% EtOAc in heptane to get desired product 230 yellow solid (850 mg, 24%). LCMS: [M+H] ⁺ : 676.38, Purity= 70%.

Synthesis of l-(3-bromophenyl)-N-(2-fluoro-4-(4-morpholino-7-((2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)-N- (methoxymethyl)methanesulfonamide (231):

[00798] To an ice cold stirred solution of l-(3-bromophenyl)-N-(2-fluoro-4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)methanesulfonamide 230 (740 mg, 1.09 mmol) in dichloromethane (8 mL) was added DIPEA (565 pL, 3.28 mmol) and chloro(methoxy)methane (168 pL, 2.19 mmol) drop wise. The resulting reaction mixture was stirred at room temperature for 3h. After completion of reaction (TLC monitoring), the reaction mass was diluted with with water (50 mL) and extracted with DCM ( 3 x 50 mL). The combined organic layer washed with brine solution, dried over Na2SO4, filtered and concentrated under reduced pressure to get desired compound 231 as yellow solid (710 mg, 90%).

LCMS: [M+H] ⁺ : 720.35, Purity= 75%. Synthesis of l-(3-bromophenyl)-N-(2-fluoro-4-(4-morpholino-7-((2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)-N- (methoxymethyl)ethane-l-sulfonamide (232):

[00799] To a solution of l-(3-bromophenyl)-N-(2-fluoro-4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-N-

(methoxymethyl)methanesulfonamide 231 (730 mg, 1.01 mmol) in tetrahydrofuran (30 mL) was cooled to -78°C, followed by addition of LiHMDS (1.0 M in THF, 2.03 mL, 2.03 mmol) dropwise. The reaction mixture was stirred at same temperature for Ih and added iodomethane (0.070 mL, 1.11 mmol) drop wise. The reaction mixture was stirred at rt for 2h. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice cold water (100 mL) and extracted with ethyl acetate (3 x 200 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified over silica gel (100- 200M) eluted with 35% ethyl acetate in heptane to get the desired product as brown foamy solid 232 (330 mg, 44%). LCMS: [M+H] ⁺ : 734.09, Purity= 70%.

Synthesis of tert-butyl ((3R)-l-(3-(l-(N-(2-fluoro-4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-N- (methoxymethyl)sulfamoyl)ethyl)phenyl)piperidin-3-yl)carbama te (233):

[00800] To a stirred solution of l-(3-bromophenyl)-N-{2-fhioro-4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-N-

(methoxymethyl)ethane-l -sulfonamide 232 (330 mg, 449 pmol) and tert-butyl N-[(3R)-piperidin-3- yl]carbamate 8 (180 mg, 898 pmol) in toluene (13 mL) was added CS2CO3 (439 mg, 1.35 mmol). The resulting mixture was degassed with argon for 10 min, followed by addition of Pd2(dba)3 (41.1 mg, 44.9 pmol) and RuPhos (41.9 mg, 89.8 pmol). The reaction mixture was heated at 100°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was cooled and filtered through celite bed, washed with ethyl acetate (2 x 50 mL). The combined organic part was washed with water, brine solution respectively, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) eluted with 45% EtOAc in heptane to get desired product as pale yellow solid 233 (0.22 g, 57%). LCMS: [M+H]+: 854.62, Purity = 80%. Synthesis of l-(3-((R)-3-aminopiperidin-l-yl)phenyl)-N-(2-fluoro-4-(4-mor pholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)ethane-l-sulfonamide (235):

[00801] To an ice cold solution of tert-butyl ((3R)-l-(3-(l-(N-(2-fluoro-4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-N-

(methoxym ethyl)sulfamoyl)ethyl)phenyl)piperi din-3 -yl)carbamate 233 (220 mg, 258 pmol) in ethyl acetate (2 mL) was added hydrogen chloride (12N, 0.25 mL) and reaction mixture was stirred at room temperature for 6h. After completion of starting material (TLC monitoring), reaction mixture was concentrated under reduced pressure. The crude was basify with saturated solution of NaHCOs and extracted with 20% IPAiCHCh (2 x 100 mL). The combined organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get crude 234. The crude was again dissolved in dichloromethane (5 mL) was cooled to 0°C followed by addition of trifluoroacetic acid (2 mL) and the reaction mixture was stirred at room temperature for 3h. After completion of reaction (TLC monitoring), the reaction mass was concentrated under reduced pressure. The crude was dissolved in 25% IPA:CHC13 (100 mL) and washed with saturated solution of NaHCOs (2 x 50 mL). The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get desired product as light brown solid 235 (120 mg, 80%). LCMS: [M+H]+: 580.35, Purity= 74%.

Synthesis of N-((3R)-l-(3-(l-(N-(2-fluoro-4-(4-morpholino-7H-pyrrolo[2,3- d]pyrimidin-6- yl)phenyl)sulfamoyl)ethyl)phenyl)piperidin-3-yl)acrylamide (559):

[00802] To an ice cold stirred solution of l-(3-((R)-3-aminopiperidin-l-yl)phenyl)-N-(2-fluoro-4-(4- morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)ethane-l-s ulfonamide 235 (0.1 g, 173 pmol) in dimethylformamide (2 mL) was added triethylamine (72.7 pL, 518 pmol) and prop-2-enoyl chloride 27 (15.6 mg, 173 pmol) and stirred reaction mixture for 10 min at same temperature. After completion of reaction (TLC and LCMS monitoring), the resulting reaction mass was evaporated up to complete dryness. The crude residue was purified through RP-HPLC purification to get the desired product as off white solid 559 (13 mg, Yield: 12%).

¹ H NMR (400 MHz, DMSO-d6): 5 12.23 (s, 1H), 9.67 (s, 1H), 8.18 (s, 1H), 8.10 (d, J= 7.6 Hz, 1H), 7.81 (d, J= 12.4 Hz, 1H), 7.64 (d, J = 8.4 Hz, 1H), 7.35-7.22 (m, 2H), 7.17-7.13 (m, 1H), 6.93-6.87 (m, 2H), 6.81-6.79 (m, 1H), 6.28-6.21 (m, 1H), 6.13-6.08 (m, 1H), 5.59 (dd, J= 2.4 & 10.0 Hz, 1H), 4.43-4.39 (m, 1H), 3.88-3.85 (m, 4H), 3.82 (m, 1H), 3.75-3.74 (m, 4H), 3.57-3.55 (m, 1H), 3.45-3.42 (m, 1H), 2.73 (m, 1H), 1.82-1.80 (m, 2H), 1.75-1.72 (m, 2H), 1.68-1.66 (m, 2H), 1.56-1.50 (m, 1H) and 1.42-1.39 (m, 1H). LCMS: [M+H] ⁺ : 634.45, Purity= 98.70%. Example B0700-46

Synthesis of Compound B0700-561

[00803] Compound B0700-561 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-N-(l-(2-(((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)amino)methyl)isonicotinoyl)piperidin-3-yl)acrylami de (561):

Synthesis of tert-butyl (R)-(l-(2-bromoisonicotinoyl)piperidin-3-yl)carbamate (242):

[00804] To an ice-cold stirred solution of 2-bromoisonicotinic acid 241 (1.00 g, 4.95 mmol) and tertbutyl (R)-piperi din-3 -ylcarbamate 8 (991 mg, 4.95 mmol) in N,N-dimethylformamide (10 mL) was added DIPEA (4.21 mL, 24.8 mmol) and T3P (50% EtOAc, 4.46 mL, 14.9 mmol). The resulting reaction mixture was stirred at room temperature for 16h under N2 atmosphere. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice-cold water (200 mL) and extracted with EtOAc (2 x 200 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 12 g SNAP) eluted with 70% ethyl acetate in heptane to get the desired product as white solid 242 (1.30 g, 68%).

LCMS: [M+H] ⁺ : 384, Purity= 99%. Synthesis of tert-butyl (R)-(l-(2-vinylisonicotinoyl)piperidin-3-yl)carbamate (244):

[00805] To a stirred solution of tert-butyl (R)-(l-(2-bromoisonicotinoyl)piperidin-3-yl)carbamate 242 (1.30 g, 3.38 mmol) in 1,4-dioxane (12.0 mL) was added 2-ethenyl-4,4,5,5-tetramethyl-l,3,2- dioxaborolane 243 (521 mg, 3.38 mmol) and Na2CO3(717 mg, 6.77 mmol) in water (3.00 mL). The resulting reaction mixture was purged with argon for 15 min, followed by addition of PdC12(dppf) (237 mg, 338 pmol). The resulting reaction mixture was stirred at 100°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with ethyl acetate (200 mL) and passed through celite bed. The filtrate was washed with water (400 mL), organic layer was dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) eluted with 70% ethyl acetate in heptane to get the desired product as white solid 244 (540 mg, 48%). LCMS: [M+H] ⁺ : 332.28, Purity= 95%.

Synthesis of tert-butyl (R)-(l-(2-formylisonicotinoyl)piperidin-3-yl)carbamate (245):

[00806] To a stirred solution of tert-butyl (R)-(l-(2-vinylisonicotinoyl)piperidin-3-yl)carbamate 244 (540 mg, 1.45 mmol) in DCM (10.0 mL) was cooled to at -78°C and passed the ozone gas for 15 min. After completion of reaction (TLC monitoring), the reaction mixture was quenched with dimethyl sulfide, diluted with water (50 mL) and extracted with DCM (2 x 30 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and concentrated under reduced pressure to get crude 245, which was used as such for next step without purification (380 mg).

Synthesis of tert-butyl (R)-(l-(2-(((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)me thyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)amino)methyl)isonicotino yl)piperidin-3-yl)carbamate (246):

[00807] To an ice-cold solution of tert-butyl N-[(3R)-l-(2-formylpyridine-4-carbonyl)piperidin-3- yl]carbamate 245 (350 mg, 1.05 mmol) in 1,2-di chloroethane (5.0 mL) was added 4-[4-(morpholin-4- yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]py rimidin-6-yl]aniline 7 (223 mg, 525 pmol) and sodium acetate (172 mg, 2.10 mmol). The resulting reaction mixture was stirred at rt for 3h under N2 atmosphere. After completion of reaction (TLC monitoring), cooled the reaction mass, at 0°C, followed by addition of STAB (445 mg, 2.10 mmol). The reaction mixture was stirred at RT for 16h. After completion of reaction (TLC and LCMS monitoring ), the reaction mixture was diluted with water (50 mL) and extracted with DCM (2 x 50 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica-gel, 12 g SNAP) eluted with 5% MeOH in DCM to get desired product as yellow solid 246 (200 mg, 25%). LCMS: [M+H] ⁺ : 743.40, Purity= 82%.

Synthesis of (R)-(3-aminopiperidin-l-yl)(2-(((4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)amino)methyl)pyridin-4-yl)methanone (247):

[00808] To an ice cold solution of tert-butyl (R)-(l-(2-(((4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)amino)methyl)isonicotinoyl )piperi din-3 -yl)carbamate 246 (200 mg, 283 pmol) in dichloromethane (5.0 mL) was added trifluoroacetic acid (1.00 mL) dropwise. The resulting reaction mixture was stirred at room temp for 3h under N2 atmosphere. After completion of reaction (TLC and LCMS monitoring) the reaction mixture was concentrated under reduced pressure. The crude residue was dissolved in 1,4-dioxane (5.00 mL), added ethane-l,2-diamine (54.2 mg, 902 pmol) and reaction mixture was heated at 80°C for Ih. After completion of reaction (LCMS monitoring) the reaction mixture was concentrated under reduced pressure. The crude was diluted with water (30.0 mL) and extracted with 25% IPAiCHCh (2 x 30 mL). The combined organic layer was dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get brown viscous liquid 247 (120 mg, 87%). LCMS: [M+H] ⁺ : 513.25 , Purity= 72%.

Synthesis of (R)-N-(l-(2-(((4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)amino)methyl)isonicotinoyl)piperidin-3-yl)acrylami de (561):

[00809] To an ice-cold stirred solution of (R)-(3-aminopiperidin-l-yl)(2-(((4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)amino)methyl)pyridin-4-y l)methanone 247 (80 mg, 156 pmol) in tetrahydrofuran (2.0 mL) was added potassium phosphate tribasic (33.1 mg, 156 pmol) in water (0.5 mL) dropwise. The reaction mixture was stirred for 15 min at same temperature under N2 atmosphere, followed by addition of a stock solution of 3-chloropropanoyl chloride (5.94 mg, 46.8 pmol) in tetrahydrofuran (0.5 mL) to the reaction mixture and stirred for 15 min. After completion of reaction (LCMS monitoring) the reaction mixture was diluted with water (10 mL) and extracted with 25% IPA:CHC13 (3 x 10 mL). The combined organic layer was washed with brine solution, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get crude which was purified through RP-HPLC purification to get desired product 561 (4.8 mg, 5.4%).

¹ H NMR (400 MHz, DMSO-d6): 5 11.90 (s, IH), 8.63-8.55 (m, IH), 8.12 (s, 2H), 7.61-7.59 (m, 2H), 7.32 (s, IH), 7.26-7.20 (m, IH), 6.83 (s, IH), 6.65-6.63 (m, 2H), 6.27-6.21 (m, IH), 6.11-6.07 (m, 2H), 5.60-5.55 (m, IH), 4.44-4.43 (m, 2H), 3.83-3.82 (m, 4H), 3.73-3.72 (m, 6H), 3.32 (m, IH), 2.98- 2.94 (m, 1H), 1.83-1.81 (m, 1H), 1.58 (m, 3H) and 1.37 (m, 1H). LCMS: [M+H] ⁺ : 567.40 , Punty= 92%.

Example B0700-48

Synthesis of Compound B0700-563

[00810] Compound B0700-563 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (S)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-morpholin o-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide (563):

Methods of normal phase separation of 563 from 248 (racemic):

Column name: Chiralpak IG (21x250mm) 5 pm.

Mobile phase and flow rate: Ethyl acetate:IPA (50:50), flow rate=18.0 mL/min.

^X H NMR (400 MHz, DMSO-d6): 5 12.19 (br s, 1H), 10.73 (s, 1H), 8.68 (d, J= 4.8 Hz, 1H), 8.18 (s, 1H), 8.11 (s, 1H), 8.00-7.96 (m, 3H), 7.92 (d, J= 8.8 Hz, 1H), 7.63 (d, J= 4.0 Hz, 1H), 7.16 (d, J = 1.6 Hz, 1H), 7.25-7.18 (m, 1H), 6.07-6.02 (m, 1H), 5.57 (d, J = 2.0 & 10.0 Hz, 1H), 3.90-3.89 (m, 4H), 3.83-3.82 (m, 1H), 3.76-3.74 (m, 4H), 3.66 (s, 2H), 2.80-2.77 (m, 1H), 2.65-2.64 (m, 1H), 2.07- 2.01 (m, 1H), 1.90-1.88 (m, 1H), 1.78-1.68 (m, 2H), 1.55-1.52 (m, 1H) and 1.23-1.14 (m, 2H). LCMS: [M-H] ⁺ : 565.18, Purity: 99.30%, ee purity: 99.18%

- 261 -

RECTIFIED SHEET (RULE 91 ) ISA/EP Example B0700-49

Synthesis of Compound B0700-567

[00811] Compound B0700-567 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of 5-((3-acrylamidoazetidin-l-yl)methyl)-6-chloro-N-(4-(4-morph olino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (567):

Synthesis of methyl 6-chloro-5-formylpicolinate (250):

[00812] To an ice cold solution of triphosgene (4.13 g, 13.98 mmol) in N,N-dimethylformamide (7.6 mL, 97.86 mmol) was added methyl 2-acetamidoacrylate 249 (2.0 g, 13.98 mmol). The reaction mixture was stirred at room temperature for 2h. The reaction mixture was refluxed at 75°C for 5h. After completion of starting material (TLC monitoring), reaction mixture was poured into ice water (50 mL) and extracted with DCM (2 x 100 mL). The combined organic layer washed with brine solution, dried over Na2SO4, filtered and concentrated under reduced pressure. The crude was purified by column chromatography silica gel (40 g SNAP) eluted with 10-15% EtOAc in heptane to get desired product as off white solid 250 (560 mg, 20%). ^X H NMR (400 MHz, CDCh): 5 10.49 (s, 1H), 8.37 (d, J = 8.0 Hz, 1H), 8.20 (d, J = 8.0 Hz, 1H) and 4.04 (s, 3H).

Synthesis of methyl 5-((3-((tert-butoxycarbonyl)amino)azetidin-l-yl)methyl)-6-ch loropicolinate

(251):

[00813] To a stirred solution of methyl 6-chloro-5-formylpyridine-2-carboxylate 250 (560 mg, 2.81 mmol) in di chloroethane (8 mL) was added tert-butyl N-(azetidin-3-yl)carbamate 105 (725 mg, 4.21 mmol) and sodium acetate (460 mg, 5.61 mmol). The reaction mixture was stirred for 2h at room temperature, followed by the addition of STAB (1.19 g, 5.61 mmol) and reaction mixture was stirred at same temperature for 16h. After the completion of reaction (TLC monitoring), the reaction mixture was diluted with water (100 mL) and extracted with DCM (2 x 100 mL). The combined organic layer was dried over Na2SO4, filtered and evaporated under reduced pressure to get desired product as yellow solid 251 (550 mg, 56%). LCMS: [M+H] ⁺ : 356.22, Purity=97.04%.

Synthesis of 5-((3-((tert-butoxycarbonyl)amino)azetidin-l-yl)methyl)-6-ch loropicolinic acid

(252):

[00814] To an ice cold stirred solution of methyl 5-[(3-{[(tert-butoxy)carbonyl]amino}azetidin-l- yl)methyl]-6-chloropyridine-2-carboxylate 251 (550 mg, 1.55 mmol) in THF (7.0 mL) and H2O (3.5 mL) was added LiOH.H2O (148 mg, 6.18 mmol). The reaction mixture was stirred at room temperature for 16h. After the completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure to get desired product as off white solid as lithium salt 252 (730 mg, qunatitative). LCMS: [M-H]': 340.13, Purity=97.79%.

Synthesis of tert-butyl (l-((2-chloro-6-((4-(4-morpholino-7-((2-(trimethylsilyl)etho xy)methyl)- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-3-y l)methyl)azetidin-3- yl)carbamate (253):

[00815] To an ice cold stirred solution of 5-((3-((tert-butoxycarbonyl)amino)azetidin-l-yl)methyl)- 6-chloropicolinic acid 252 (730 mg, 2.14 mmol) and 4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)aniline 7 (545 mg, 1.28 mmol) in N,N-dimethylformamide (5 mL) was added DIPEA (1.10 mL, 6.41 mmol) and HATU (1.06 g, 2.78 mmol). The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mass was poured into ice-cold water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 50% ethyl acetate in heptane to get the desired product as brown gummy solid 253 (700 mg, 62%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 5 10.55 (s, 1H), 8.27 (s, 1H), 8.14-8.12 (s, 1H), 8.08-8.06 (m, 1H), 8.03-8.01 (m, J= 8 Hz, 2H), 7.79 (d, J= 8.0 Hz, 2H), 7.69 (m, 1H), 6.96 (s, 1H), 5.57 (s, 2H), 4.06- 4.04 (m, 3H), 3.97-3.89 (m, 4H), 3.81-3.74 (m, 6H), 3.61-3.64 9 (m, 4H), 1.37 (s, 9H), 0.89-0.85 (m, 2H) and -0.06 (s, 9H). LCMS: [M+H] ⁺ : 749.29, Purity= 88%.

Synthesis of 5-((3-aminoazetidin-l-yl)methyl)-6-chloro-N-(4-(4-morpholino -7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide (254):

[00816] To an ice cold solution of tert-butyl (l-((2-chloro-6-((4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyri din-3- yl)methyl)azetidin-3-yl)carbamate 253 (300 mg, 0.4 mmol) in DCM (6 mL) was added TFA (3.0 mL) in drop wise. The resulting reaction mixture was allowed to stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The crude residue was treated with aqeous NH4OH solution to get solid precipitates, filtered and dried under vaccum to get the desired product as brown solid 254 (195 mg; 93%). LCMS: [M+H] ⁺ : 519.21.

Synthesis of 5-((3-acrylamidoazetidin-l-yl)methyl)-6-chloro-N-(4-(4-morph olino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (567):

[00817] To an ice-cold stirred solution of 5-((3-aminoazetidin-l-yl)methyl)-6-chloro-N-(4-(4- morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinami de 254 (160 mg, 308 pmol) in 1,4- dioxane (4 mL) was added triethylamine (93.6 mg, 925 pmol) and prop-2-enoyl chloride 27 (30.7 mg, 339 pmol) in drop wise. The reaction mixture was stirred at same temperature for 30 min. After completion of reaction (TLC monitoring), the resulting reaction mass was evaporated to dryness and the crude residue was purified through RP-HPLC purification using eluent as 5mM ammonium bicarbonate in water/acetonitrile and column using Waters X-select Phenyl -Hexyl(19*250mm, 5pm to get desired product as pale yellow solid 567 (4.60 mg, 3%).

^X H NMR (400 MHz, DMSO-d6): 8 12.21 (br, s, 1H), 10.48 (s, 1H), 8.61 (d, J = 4.0 Hz, 1H), 8.18 (s, 1H), 8.16-8.08 (m, 2H), 7.97-7.91 (m, 4H), 7.17 (s, 1H), 6.24- 6.21 (m, 1H), 6.11-6.07 (m, 1H), 5.63- 5.60 (m, 1H), 4.45-4.37 (m, 1H), 3.89-3.88 (m, 4H), 3.79 (s, 2H), 2.76-2.75 (m, 4H), 3.68-3.65 (m, 2H) and 3.08-3.05 (m, 2H). LCMS: [M+H] ⁺ : 573.39, Purity= 95.30% Example B0700-50

Synthesis of Compound B0700-532

[00818] Compound B0700-532 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-N-(l-(3-(N-(4-(7-morpholinothiazolo[5,4-d]pyrimidin-2- yl)phenyl)sulfamoyl)benzyl)piperidin-3-yl)acrylamide (532):

Synthesis of 4-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)aniline (255):

[00819] To a stirred solution of 4-(2-bromothiazolo[5,4-d]pyrimidin-7-yl)morpholine 99 (2.50 g, 8.30 mmol) and (4-aminophenyl)boronic acid 6 (1.36 g, 9.96 mmol) in N,N-dimethylformamide (40 mL) was added K3PO4 (4.34 g, 24.9 mmol) in water (10.0 mL) under nitrogen degassing, followed by addition of palladium bis(triphenylphosphane) dichloride (408 mg, 581 pmol). The resultant reaction mixture was stirred at 100°C for 16h. After the completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed and washed with ethyl acetate (200 mL). The organic part was diluted with water (200 mL) and extracted with ethyl acetate (2 x 200 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 50% Ethyl acetate in heptane to get the desired product as yellow solid 255 (2.0 g, 76.88%). 'H NMR (400 MHz, DMSO-t/ ₆ ): 8.34 (s, 1H), 7.70 (d, J= 8.0 H _Z , 2H), 6.66 (d, J= 8.0 H _Z , 2H), 4.30 (s, 2H), 3.77-3.76 (m, 4H) and 3.17-3.16 (m, 4H). LCMS: [M+H] ⁺ : 314.14, Purity= 73%.

Synthesis of methyl 3-(N-(4-(7-morpholinothiazolo[5,4-d]pyrimidin-2- yl)phenyl)sulfamoyl)benzoate (256):

[00820] To an ice cold solution of 4-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)aniline 255 (2.0 g, 6.38 mmol) in dichloromethane (50 mL) was added pyridine (2.52 g, 31.9 mmol), followed by addition of methyl 3-(chlorosulfonyl)benzoate 140 (1.50 g, 6.38 mmol). The resultant reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mass diluted with water (100 mL) and extracted with DCM (3 X 100 mL). The combined organic layer was washed with brine solution (100 mL), dried over anhydrous Na2SO4 and concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 60% ethyl acetate in heptane to get desired product as a white solid 256 (2 g, 61.26%.

'H NMR (400 MHz, DMSO-</ ₆ ): 8 10.94 (s, 1H), 8.39 (s, 1H), 8.39 (d, J= 8.0 Hz, 1H), 8.18 (d, J= 8.0 H _z , 1H), 8.08 (d, J= 6.8 Hz, 1H), 7.94 (d, J = 8.8 H _Z , 2H), 7.76 (t, J= 8 Hz, 1H), 7.28 (d, J = 8.4 H _Z , 2H), 4.45-4.38 (m, 4H), 3.87 (s, 3H) and 3.74 (m, 4H). LCMS: [M+H] ⁺ : 512.11, Purity= 93.33%. Synthesis of 3-(hydroxymethyl)-N-(4-(7-morpholinothiazolo[5,4-d]pyrimidin -2- yl)phenyl)benzenesulfonamide (257):

[00821] To an ice-cold solution of methyl 3-(N-(4-(7-morpholinothiazolo[5,4-d]pyrimidin-2- yl)phenyl)sulfamoyl)benzoate 256 (2.0 g, 3.91 mmol) in THF (20 mL) was added LAH (2 M in THF, 2.93 mL, 5.86 mmol). The resultant reaction mixture was stirred at room temperature for 5h. After completion of starting material (TLC monitoring), reaction mixture was cooled at 0°C added ice cold water (10 mL) and 15% NaOH solution (10 mL) dropwise. The reaction mixture was filtered through celite bed and washed with EtOAc (3 x 100 mL). The combined filterate was dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get desired product as white solid 257 (1.8 g, 95.21%). LCMS: [M+H] ⁺ : 484.16, Purity= 81.70%.

Synthesis of 3-formyl-N-(4-(7-morpholinothiazolo [5,4-d] pyrimidin-2- yl)phenyl)benzenesulfonamide (258):

[00822] To an ice-cold solution of 3-(hydroxymethyl)-N-(4-(7-morpholinothiazolo[5,4-d]pyrimidin - 2-yl)phenyl)benzenesulfonamide 257 (1.00 g, 2.07 mmol) in acetonitrile (30 mL) was added PCC (669 mg, 3.10 mmol). The reaction mixture was stirred at 80°C for 2h under nitrogen atmosphere. After completion of starting material (TLC monitoring), the reaction mixture was filtered through sintered funnel through celite bed and washed with 10% MeOH:DCM (250 mL). The filtrate was concentrated under reduced pressure. The crude residue was dissolved in water (100 mL) and extracted with 10% MeOH:DCM (2 x 200 mL). The combined organic layer were washed with brine (100 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get desired product as yellow solid 258 (900 mg, 90.38%). LCMS: [M+H] ⁺ : 482.21, Purity= 74.07%.

Synthesis of tert-butyl (R)-(l-(3-(N-(4-(7-morpholinothiazolo[5,4-d]pyrimidin-2- yl)phenyl)sulfamoyl)benzyl)piperidin-3-yl)carbamate (259):

[00823] To an ice cold solution of 3-formyl-N-(4-(7-morpholinothiazolo[5,4-d]pyrimidin-2- yl)phenyl)benzenesulfonamide 258 (900 mg, 1.87 mmol) and tert-butyl (R)-piperi din-3 -ylcarbamate 9 (749 mg, 3.74 mmol) in 1,2-di chloroethane (10 mL) was added sodium acetate (460 mg, 5.61 mmol). The resulting reaction mixture was stirred at same temperature for Ih, followed by addition of STAB (1.18 g, 5.61 mmol). The reaction mixture was allowed to stirred at room temperature for 16h. After completion of reaction (TLC monitoring), solvent was evaporated up to dryness under reduced pressure. The crude residue was diluted with ice-cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed with brine solution, dried over ISfeSCU, filtered and solvent was evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12g SNAP) eluted with 70% ethyl acetate in heptane to get the desired product as light yellow solid 259 (700 mg, 56%). LCMS: [M+H] ⁺ : 666.39, Purity= 93.48%.

Synthesis of (R)-3-((3-aminopiperidin-l-yl)methyl)-N-(4-(7-morpholinothia zolo[5,4- d]pyrimidin-2-yl)phenyl)benzenesulfonamide (260):

[00824] To an ice cold stirred solution of tert-butyl (R)-(l-(3-(N-(4-(7-morpholinothiazolo[5,4- d]pyrimidin-2-yl)phenyl)sulfamoyl)benzyl)piperidin-3-yl)carb amate 259 (700 mg, 1.05 mmol) in dichloromethane (10.0 mL) was added trifluoroacetic acid (5.00 mL). The resulting reaction mixture was stirred at room temperature for 4h. After completion of reaction (TLC monitoring), the reaction mass was concentrated under reduced pressure. The crude residue was triturated with diethyl ether to get desired product 260 (1.0 g, TFA salt). LCMS: [M+H] ⁺ : 566.28, Purity= 88.86%.

Synthesis of (R)-N-(l-(3-(N-(4-(7-morpholinothiazolo [5,4-d] pyrimidin-2- yl)phenyl)sulfamoyl)benzyl)piperidin-3-yl)acrylamide (532):

[00825] To an ice cold stirred solution of (R)-3-((3-aminopiperidin-l-yl)methyl)-N-(4-(7- morpholinothiazolo[5,4-d]pyrimidin-2-yl)phenyl)benzenesulfon amide 260 (600 mg, 1.06 mmol) in DMF (6.00 mL) was added triethylamine (870 pL, 6.36 mmol) and a solution of prop-2-enoyl chloride 27 (0.1 mL, 1.06 mmol) in DCM (1 mL). The resulting reaction mixture was stirred at 0°C for 10 min. After completion of reaction (TLC monitoring) added ice cold water (50 mL) and extracted with 10% MeOH in DCM (3 x 100 mL). The combined organic layer was washed with brine, dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude residue was purified by silica-gel flash column chromatography (10% MeOH/DCM) to get desired product as a white solid 532 (160 mg, 24.34%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 5 10.47 (s, 1H), 8.39 (s, 1H), 7.95-7.89 (m, 3H), 7.76 (s, 1H), 7.71- 7.69 (m, 1H), 7.52-7.50 (m, 2H), 7.27-7.25 (m, 2H), 6.23-6.16 (m, 1H), 6.07-6.02 (m, 1H), 5.55-5.52 (m, 1H), 4.32-4.29 (m, 4H), 3.78-3.75 (m, 5H), 3.55-3.52 (m, 2H), 2.74-2.73 (m, 2H), 1.98-1.92 (m, 2H), 1.72-1.68 (m, 2H), 1.58-1.49 (m, 1H) and 1.36-1.32 (m, 1H). LCMS: [M+H] ⁺ : 620.47, Purity= 96%.

Example B0700-51

Synthesis of Compound B0700-534

[00826] Compound B0700-534 was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-N-(l-(3-(N-(6-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl )pyridin-3- yl)sulfamoyl)benzyl)piperidin-3-yl)acrylamide (534):

Synthesis of 4-(2-(5-chloropyridin-2-yl)thiazolo[5,4-d]pyrimidin-7-yl)mor pholine (261):

[00827] To a stirred solution of 4-(2-bromothiazolo[5,4-d]pyrimidin-7-yl)morpholine 99 (1.0 g, 3.32 mmol) and (5-chloropyridin-2-yl)boronic acid 133 (2.09 g, 13.3 mmol) in N,N-dimethylformamide (20.0 mL) was added CS2CO3 (2.16 g, 6.64 mmol) and copper iodide (632 mg, 3.32 mmol). The reaction mass was degassed with nitrogen for 10 min, followed by addition of Pd(OAc)2 (37.3 mg, 166 pmol) and dppf (184 mg, 332 pmol). The resulting reaction mixture was stirred at 100°C for 16h. After the completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed followed by washing with ethyl acetate (100 mL). The filtrate was diluted with water (200 mL) and extracted with ethyl acetate (3 x 200 mL). The combined organic layer was washed with brine solution, dried over TsfeSCU, filtered and evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) eluted with 50% ethyl acetate in heptane to get the desired product as yellow solid 261 (700 mg, 63.16%).

^X H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.79 (s, 1H), 8.45 (s, 1H), 8.31 (d, J= 8.4 Hz, 1H), 8.18 (d, J = 8 Hz, 1H), 4.34 (s, 4H) and 3.78 (s, 4H). LCMS: [M+H] ⁺ : 334.12, Purity= 86%.

Synthesis of tert-butyl (6-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)pyridin-3-yl)c arbamate (262):

[00828] To a stirred solution of 4-(2-(5-chloropyridin-2-yl)thiazolo[5,4-d]pyrimidin-7-yl)mor pholine 261 (700 mg, 2.10 mmol) and tert-butyl carbamate 135 (737 mg, 6.29 mmol) in 1,4-dioxane (15.0 mL) was added CS2CO3 (957 mg, 2.94 mmol). The reaction mass was degassed with nitrogen for 10 min followed by addition of Pd(OAc)2 (28.2 mg, 126 pmol) and X-phos (200 mg, 419 pmol). The resultant reaction mixture was stirred at 100°C for 16h. After the completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed followed by washing with ethyl acetate (200 mL). The filtrate was diluted with water (200 mL) and extracted with ethyl acetate (3 x 200 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12g SNAP) eluted with 50% ethyl acetate in heptane to get the desired product as a white solid 262 (700 mg, 29.8%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 9.99 (s, 1H), 8.71 (s, 1H), 8.42 (s, 1H), 8.22 (d, J= 8.8 Hz, 1H), 8.13 (d, J= 8.8 Hz, 1H), 4.34 (s, 4H), 3.77-3.76 (m, 4H) and 1.50 (s, 9H).

Synthesis of 6-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)pyridin-3-amine (263):

[00829] To an ice cold stirred solution of tert-butyl (6-(7-morpholinothiazolo[5,4-d]pyrimidin-2- yl)pyridin-3-yl)carbamate 262 (700 mg, 1.69 mmol) in dichloromethane (10 mL) was added trifluoroacetic acid (5.0 mL). The resultant reaction mixture was stirred at room temperature for 4h. After completion of reaction (monitored by TLC), concentrated the reaction mass under reduced pressure. The crude was diluted with water (25 mL), basified with NaHCCL solution and extracted with 10% MeOH:DCM (3 x 100 mL). The combined organic layer was washed with brine solution, dried over NazSCL, filtered and evaporated under reduced pressure to get desired product brown viscous 263 (600 mg,).

'H NMR (400 MHz, DMSO-t/ ₆ ): 5 8.37 (s, 1H), 7.99 (s, 1H), 7.95-7.93 (m, 1H), 7.06-7.05 (m, 1H), 6.16 (s, 2H), 4.30 (s, 4H) and 3.77-3.76 (m, 4H). LCMS: [M+H] ⁺ : 315.14, Purity= 96%.

Synthesis of methyl 3-(N-(6-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)pyridin-3 - yl)sulfamoyl)benzoate (264):

[00830] To an ice cold stirred solution of 6-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)pyridin-3- amine 263 (600 mg, 1.91 mmol) in dichloromethane (15 mL) was added pyridine (755 mg, 9.54 mmol) and methyl 3-(chlorosulfonyl)benzoate 140 (0.55 mL, 2.29 mmol). The resultant reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), reaction mass diluted with water (100 mL) and extracted with DCM (3 X 100 mL). The combined organic layer was washed with brine solution, dried over anhydrous NazSCU filtered and concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) eluted with 70% ethyl acetate in heptane to get desired product as a white solid 264 (200 mg, 20.44%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 11.19 (s, 1H), 8.41 (s, 1H), 8.40 (s, 1H), 8.39 (d, J= 8.4 Hz, 1H), 8.21 (d, J= 8.4 Hz,2H), 8.10-8.08 (m, 1H), 7.77-7.75 (m, 2H), 4.31 (s, 4H), 3.87 (s, 3H) and 3.75 (m, 4H). LCMS: [M+H] ⁺ : 513.18, Purity= 93.78%.

Synthesis of 3-(hydroxymethyl)-N-(6-(7-morpholinothiazolo[5,4-d]pyrimidin -2-yl)pyridin-3- yl)benzenesulfonamide (265):

[00831] To an ice-cold stirred solution of methyl 3-(N-(6-(7-morpholinothiazolo[5,4-d]pyrimidin-2- yl)pyridin-3-yl)sulfamoyl)benzoate 264 (200 mg, 390 pmol) in THF (5.0 mL) was added LAH (2 M in THF) (390 pL, 780 pmol) dropwise. The resultant reaction mixture was stirred at room temperature for 5h. After completion of reaction (monitored by TLC), reaction mixture was cooled at 0°C quenched with 10% NaOH solution (10 mL) and ice cold water (10 mL). The solid precipitates was filtered through celite bed and washed with EtOAc (2 x 100 mL). The combined filterate was dried over anhydrous NazSCL. filtered and concentrated under reduced pressure to get desired product as a white solid 265 (200 mg, quantitative).

LCMS: [M+H] ⁺ : 485.20, Purity= 94%. Synthesis of 3-formyl-N-(6-(7-morpholinothiazolo [5,4-d] pyrimidin-2-yl)pyridin-3- yl)benzenesulfonamide (266):

[00832] To an ice-cold stirred solution of 3-(hydroxymethyl)-N-(6-(7-morpholinothiazolo[5,4- d]pyrimidin-2-yl)pyridin-3-yl)benzenesulfonamide 265 (200 mg, 413 pmol) in acetonitrile (5.00 mL) was added PCC (178 mg, 826 pmol). The reaction mixture was stirred at 80°C for 2h. After completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed and washed with 10% MeOH:DCM (200 mL). The filtrate was concentrated under reduced pressure. The crude residue was taken in water (50 mL) and extracted with 10% MeOH:DCM (2 x 100 mL). The combined organic layer was washed with brine (50 mL), dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure to get desired product 266 (180 mg, 90.38%). LCMS: [M+H] ⁺ : 483.13, Purity = 77%.

Synthesis of tert-butyl (R)-(l-(3-(N-(6-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl)p yridin-3- yl)sulfamoyl)benzyl)piperidin-3-yl)carbamate (267):

[00833] To an ice-cold stirred solution of 3-formyl-N-(6-(7-morpholinothiazolo[5,4-d]pyrimidin-2- yl)pyridin-3-yl)benzenesulfonamide 266 (180 mg, 373 pmol) and tert-butyl N-[(3R)-piperi din-3 - yl]carbamate 18 (149 mg, 746 pmol) in 1,2-di chloroethane (5.0 mL) was added sodium acetate (91.8 mg, 1.12 mmol). The resulting reaction mixture was stirred at same temperature for Ih, followed by addition of STAB (235 mg, 1.12 mmol) portion wise. The resulting reaction was allowed to stirred at room temperature for 16h. After completion of reaction (TLC monitoring) diluted with water (50 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (50 mL), dried over ISfeSCU, filtered and solvent was evaporated under reduced pressure. The crude crude residue was purified by flash column chromatography (silica gel, 12g SNAP) eluted with 70% ethyl acetate in heptane to get the desired product as yellow solid 267 (140 mg, 56.28%). LCMS: [M-H]’: 665.20, Purity= 77.15%.

Synthesis of (R)-3-((3-aminopiperidin-l-yl)methyl)-N-(6-(7-morpholinothia zolo[5,4- d]pyrimidin-2-yl)pyridin-3-yl)benzenesulfonamide (268):

[00834] To an ice cold stirred solution of tert-butyl (R)-(l-(3-(N-(6-(7-morpholinothiazolo[5,4- d]pyrimidin-2 -yl)pyri din-3 -yl)sulfam oyl)benzyl)piperi din-3 -yl)carbamate 267 (140 mg, 210 pmol) in dichloromethane (4.0 mL) was added trifluoroacetic acid (1.0 mL). The resultant reaction mixture was stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mass was concentrated under reduced pressure. The crude was triturated with diethyl ether and dried under vaccuo to get desired product 268 (120 mg, with TFA salt). LCMS: [M+H] ⁺ : 567.25, Purity= 75%. Synthesis of (R)-N-(l-(3-(N-(6-(7-morpholinothiazolo[5,4-d]pyrimidin-2-yl )pyridin-3- yl)sulfamoyl)benzyl)piperidin-3-yl)acrylamide (534):

[00835] To an ice cold stirred solution of (R)-3-((3-aminopiperidin-l-yl)methyl)-N-(6-(7- morpholinothiazolo[5,4-d]pyrimidin-2-yl)pyridin-3-yl)benzene sulfonamide 268 (120 mg, 212 pmol) in DMF (5.0 mL) was added triethylamine (174 pL, 1.27 mmol) and prop-2-enoyl chloride 27 (15.3 mg, 169 pmol) in DCM (1 mL). The resultant reaction mixture was stirred at 0°C for 10 min. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated under reduced pressure. The crude was basified with NH4OH solution and extracted with 20% MeOH in DCM (2 x 25 mL). The combined organic part was dried over ISfeSCU, filtered and solvent was evaported under reduced pressure. The crude was purified by silica gel column (100-200M), eluted with 15% MeOH in DCM to get the desired product as white solid 534 (20 mg, 15.22%).

¹ H NMR (400 MHz, DMSOWr,): 5 11.98 (s, 1H), 11.09 (s, 1H), 8.41 (s, 1H), 8.37 (s 1H), 8.16 (d, J= 4.0 Hz, 1H), 7.95 (d, J= 8.0 Hz, 1H), 7.75-7.66 (m, 3H), 7.53 (s, 2H), 6.22-6.16 (m, 1H), 6.06-6.02 (m, 1H), 5.55-5.52 (m, 1H), 4.30 (s, 4H), 3.75 (s, 5H), 3.59-3.45 (m, 2H), 2.74 (m, 2H), 1.80-1.75 (m, 2H), 1.65 (m, 1H), 1.57 (m, 1H) and 1.40-1.38 (m, 1H). LCMS: [M+H] ⁺ : 621.48, Purity= 97%.

Example B0700-52

Synthesis of Compound B0700-601

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(morpholi no-d8)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (601): Synthesis of tert-butyl (R)-(l-((2-((4-(4-chloro-7-((2-(trimethylsilyl)ethoxy)methyl )-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)m ethyl)piperidin-3- yl)carbamate(89):

[00836] To an ice-cold stirred solution of 4-(4-chloro-7-((2-(trimethylsilyl)ethoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)aniline 23 (1.00 g, 2.67 mmol) and 4-{[(3R)-3-{[(tert- butoxy)carbonyl]amino}piperidin-l-yl]methyl}pyridine-2-carbo xylic acid 8 (1.34 g, 4.00 mmol) in DMF (10 mL) was added DIPEA (2.33 mL, 13.3 mmol) and HATU (1.52 g, 3.92 mmol). The resulting reaction mixture was stirred at room temperature for 3h. After completion of reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and solvent was evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 30% EtOAc in Hexane to get the desired product as yellow solid 89 (1.10 g, 58%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 10.90 (s, 1H), 8.70 (t, J= 5.2 Hz, 2H), 8.14 (d, J= 8.8 Hz, 3H), 7.85 (d, J= 8.8 Hz, 2H), 7.63-7.62 (m, 1H), 6.89 (s, 1H), 6.76 (d, J= 8.0 Hz, 1H), 5.66 (s, 2H), 3.65- 3.62 (m, 2H), 3.43-3.29 (m, 1H), 2.77-2.49 (m, 2H), 1.95-1.85 (m, 2H), 1.72-1.49 (m, 1H), 1.46 (s, 9H), 1.35-.1.13 (m, 4H), 0.88-0.80 (m, 3H) and 0.08 (s, 9H). LCMS: [M+H] ⁺ : 692.3, Purity= 98.39% Synthesis of tert-butyl (R)-(l-((2-((4-(4-(morpholino-d8)-7-((2-(trimethylsilyl)etho xy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)m ethyl)piperidin-3-yl)carbamate (91): [00837] To an ice cold stirred solution of tert-butyl N-[(3R)-l-[(2-{[4-(4-chloro-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl]carbamoyl}pyri din-4- yl)methyl]piperidin-3-yl]carbamate 89 (300 mg, 433 pmol) and (2,2,3,3,5,5,6,6- ² Hs)morpholine hydrochloride 90 (171 mg, 1.30 mmol) in DMSO (5.00 mL) was added DIPEA (227 pL, 1.30 mmol). The resulting Reaction mixture was stirred at room temperature for 2h. After completion of Reaction (TLC monitoring), the resulting reaction mass was poured into ice-cold water (100 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed with brine solution, dried over Na2SO4, filtered and solvent was evaporated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 40 g SNAP) eluted with 50% EtOAc in Hexane to get the desired product as yellow solid 91 (310 mg, 95%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 10.82 (s, 1H), 8.69 (d, J= 4.8 Hz, 1H), 8.26 (s, 1H), 8.11 (s, 1H), 8.07 (d, J= 8.8 Hz, 2H), 7.78 (d, J= 8.4 Hz, 2H), 7.62 (d, J= 4.4 Hz, 1H), 6.95 (s, 1H), 6.76 (d, J= 8.0 Hz, 1H), 5.57 (s, 2H), 3.65-3.58 (m, 3H), 3.43-3.33 (m, 1H), 2.73-2.53 (m, 1H), 2.49 (s, 1H), 1.95- 1.90 (m, 2H), 1.82-1.46 (m, 2H), 1.35 (s, 9H), 1.22-1.15 (m, 3H), 0.89 (t, J= 8.0 Hz, 2H) and 0.07 (s, 9H). LCMS: [M+H] ⁺ : 751.05, Purity= 96.0%

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-(morpholino-d8 )-7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide ( 93):

[00838] To an ice cold stirred solution of tert-butyl (R)-(l-((2-((4-(4-(morpholino-d8)-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)carbamoyl)pyri din-4- yl)methyl)piperidin-3-yl)carbamate 91 (300 mg, 483 pmol) in DCM (10 mL) was added TFA (2.50 mL) in drop wise. The resulting reaction mixture was stirred at room temperature for 6h. After completion of starting material (TLC monitoring), solvent was evaporated under reduced pressure. The crude residue was dissolved in 1,4-di oxane, added ethane- 1,2-diamine 92 (0.50 mL) and the resulting reaction mixture was heated at 90°C for Ih. After completion of reaction (TLC and LCM monitoring), solvent was evaporated under reduced pressure. Thee crude residue was diluted with water (100 mL) and extracted with EtOAc (3 x 100 mL). The combined organic layer was washed with brine solution, dried over ISfeSCU, filtered and solvent was evaporated under reduced pressure to get desired product as beige solid 93 (250 mg, 93%). LCMS: [M+H] ⁺ : 521.37, Purity= 97%

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-(morpholi no-d8)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (601):

[00839] To an ice cold stirred solution of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4- (morpholino-d8)-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picol inamide 93 (250 mg, 0.48 mmol) in DMF (10 mL) was added EtsN (0.20 mL, 1.44 mmol) and acryloyl chloride 27 (51 pL, 0.58 mmol). The resulting reaction mass was stirred at same temperature for 30 minutes. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated up to dryness and the crude residue was purified through RP-HPLC purification eluted with 5mM ammonium bicarbonate in water/acetonitrile (Column: Waters Xselect Phenyl-Hexyl(19*250mm,5pm) to get desired product as white solid 601 (53 mg, 21%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 12.19 (s, IH), 10.73 (s, IH), 8.69 (d, J= 4.80 Hz, IH), 8.17 (s, IH), 8.11 (s, IH), 8.01-7.97 (m, 3H), 7.92 (d, J= 8.8 Hz, 2H), 7.63 (d, J= 4.4 Hz, IH), 7.15 (s, IH), 6.25-6.18 (m, IH), 6.07-6.02 (m, IH), 5.57-5.54 (m, IH), 3.83-3.81 (m, IH), 3.66 (s, 2H), 2.79-2.64 (m, 2H), 2.07-1.88 (m, 2H), 1.78-1.68 (m, 2H), 1.58-1.49 (m, IH) and 1.23-1.14 (m, IH). LCMS: [M+H] ⁺ : 575.11, Purity= 99.09%. Example B0700-53

Synthesis of Compound B0700-602

Preparation of (R)-N-(l-((2-(((4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)cyclohexyl)amino)methyl)pyridin-4-yl)methyl)piperidin-3-y l)acrylamide (B0700-602):

Synthesis of tert-butyl (R)-(l-((2-bromopyridin-4-yl)methyl)piperidin-3-yl)carbamate (256):

[00840] To a stirred solution of 2-bromopyridine-4-carbaldehyde 255 (3.00 g, 16.1 mmol) in di chloroethane (60.0 mL) was added tert-butyl N-[(3R)-piperidin-3-yl]carbamate 18 (4.85 g, 24.2 mmol) and sodium acetate (2.65 g, 32.3 mmol) under N2 atmosphere. The resulting reaction mixture was stirred for Ih, followed by addition of sodium triacetoxy borohydride (6.84 g, 32.3 mmol). The resulting reaction mixture was allowed to stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (100 mL), dried over Na2 SO4. filtered and concentrated under reduced pressure. The crude was purified by column chromatography silica gel (40 g SNAP) eluted with 10-15% EtOAc in heptane to get desired product as off white solid 256 (4.79 g, 77%).

'H NMR (400 MHz, DMSO-t/ ₆ ): 8 8.32-8.31 (d, J = 4.4 Hz, IH), 7.56 (s, IH), 7.38-7.37 (d, J =4.8 Hz IH), 6.77-6.75 (d, J =7.2 Hz IH), 3.54-3.44 (m, 2H), 3.39 (m, IH), 2.50 (m, IH), 1.91-1.78 (m, 2H), 1.66-1.60 (m, 2H), 1.45-1.42 (m, IH), 1.35 (s, 9H), and 1.28 (m, IH). LCMS: [M+H] ⁺ : 370.17, Purity= 97%. Synthesis of tert-butyl (R)-(l-((2-vinylpyridin-4-yl)methyl)piperidin-3-yl)carbamate (257): [00841] To a stirred solution of tert-butyl N-[(3R)-l-[(2-bromopyridin-4-yl)methyl]piperidin-3- yl]carbamate 256 (2.00 g, 5.40 mmol) in 1,4-dioxane (21.0 mL) was added 2-ethenyl-4,4,5,5- tetramethyl-l,3,2-dioxaborolane 243 (749 mg, 4.86 mmol) and lSfeCCh (1.14 g, 10.8 mmol) dissolved in H2O (7.00 mL) under nitrogen atmosphere. The resulting reaction mixture was degassed with argon gas for 10 min. Then added PdC12(dppf) (198 mg, 270 pmol) to the reaction mixture. The reaction mixture was stirred at 80°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (2 x 100 mL). The combined organic layer was washed with brine solution (100 mL), dried over ISfeSCU, filtered and concentrated under reduced pressure. The crude was purified by column chromatography silica gel (100-200M, 40 g SNAP) eluted with 20-25% EtOAc in heptane to get desired product as off white solid 257 (1.50 g, 87%).

LCMS: [M+H] ⁺ : 318.13, Purity= 99%.

Synthesis of tert-butyl (R)-(l-((2-formylpyridin-4-yl)methyl)piperidin-3-yl)carbamat e (258): [00842] To a stirred solution of tert-butyl N-[(3R)-l-[(2-ethenylpyridin-4-yl)methyl]piperidin-3- yl]carbamate 257 (500 mg, 1.58 mmol) in THF (10.0 mL) was added OsCU (440 pL, 69.3 pmol) and NalCh (674 mg, 3.15 mmol) dissolved in water (3 mL) at RT under N2 atmosphere. The reaction mixture was stirred at RT for 2h. After completion of reaction (TLC monitoring) the reaction mixture was diluted with water (50 mL) and extracted with Ethyl acetate (2 x 50 mL). The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure to get the desired product as brown solid (431 mg). The crude was used as such without purification for next step.

LCMS: [M+H] ⁺ : 370.17, Purity= 97%.

Synthesis of tert-butyl (R)-(l-((2-(((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)m ethyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)cyclohexyl)amino)methyl)pyridin -4-yl)methyl)piperidin-3- yl)carbamate (259):

[00843] To a stirred solution of tert-butyl N-[(3R)-l-[(2-formylpyridin-4-yl)methyl]piperidin-3- yl]carbamate 258 (420 mg, 1.31 mmol) in methanol (10.0 mL) was added 4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)cyclohexan-l-amine 198 (397 mg, 920 pmol) and the reaction mixture was stirred at RT for Ih under N2 atmosphere. Then NaBEL (70.6 mg, 1.97 mmol) was added to the reaction mixture and stirred at RT for 2h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure. Crude residue was then dissolved in water (30 mL) and extracted with DCM (2 x 30 mL). The combined organic layer was washed with brine solution, dried over TsfeSCU, filtered and concentrated under reduced pressure to get crude. The crude was purified by column chromatography silica gel (12 g SNAP) eluted with 5-8% MeOH in DCM to get desired product as brown solid 259 (400 mg, 42%). MS: [M+H] ⁺ : 735.58.

Synthesis of (R)-l-((2-(((4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)cyclohexyl)amino)methyl)pyridin-4-yl)methyl)piperidin-3-a mine (260):

[00844] To an ice-cold stirred solution of tert-butyl N-[(3R)-l-({2-[({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]cyclohexyl}amino)methyl]pyridin- 4-yl}methyl)piperidin-3-yl]carbamate 259 (400 mg, 544 pmol) in dichloromethane (4.00 mL) was added trifluoroacetic acid (1.00 mL) under N2 atmosphere. The resulting reaction mixture was stirred at RT for 5h. After completion of reaction (LCMS monitoring) the reaction mixture was concentrated under reduced pressure to get crude. Crude was then basified with Aqueous ammonia and extracted with 10% IPA in CHCL (3 x 50 mL). The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get the desired product as blackish solid 260 (174 mg, 64%).

MS: [M+H] ⁺ : 505.43.

Synthesis of (R)-N-(l-((2-(((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6 - yl)cyclohexyl)amino)methyl)pyridin-4-yl)methyl)piperidin-3-y l)acrylamide (B0700-602): [00845] [0013] To an ice-cold stirred solution of (3R)-l-({2-[({4-[4-(morpholin-4-yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl]cyclohexyl}amino)methyl]pyridin -4-yl}methyl)piperi din-3 -amine 260 (174 mg, 345 pmol) in THF (3.00 mL) was added K3PO4 (73.2 mg, 345 pmol) dissolved in water (2.00 mL) under N2 atmosphere. The reaction mixture was stirred for 15 min at same temperature. Then added stock solution of 3-chloropropanoyl chloride 122 (16.5 pL, 172 pmol) in THF (1.00 mL) dropwise to the reaction mixture. The resulting reaction mixture was stirred for next 30 min at 0°C. After LCMS monitoring, 2N sodium hydroxide (1.03 mL, 2.07 mmol) was added to the reaction mixture. The reaction mixture was stirred at RT for 6h. After completion of reaction (LCMS monitoring) the reaction mixture was diluted with water (20 mL) and extracted with 25% IPA in CHCh (2 x 20 mL). The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. The crude residue was purified through RP-HPLC purification using eluent as 5mM ammonium bicarbonate in water/acetonitrile and column using Waters Xselect Phenyl -Hexyl(19*250mm, 5pm to get desired product as pale yellow solid B0700- 602 (11 mg, 5%).

'H NMR (400 MHz, DMSO-d6): 8 11.54 (s, 1H), 8.41-8.40 (d, J= 4.4 Hz, 1H), 8.10 (s, 1H), 7.94- 7.92 (d, J= 8.02 Hz, 1H), 7.38 (s, 1H), 7.18 (s, 1H), 6.30 (s, 1H), 6.27-6.18 (m, 1H), 6.07-6.02 (m, 1H), 5.56-5.53 (m, 1H), 3.86 (m, 3H), 3.82-3.81 (m, 4H), 3.79-3.77 (m, 4H), 3.51 (s, 1H), 3.48-3.46 (m, 1H), 2.84 (m, 1H), 2.76-2.72 (m, 2H), 2.61-2.58 (m, 2H), 2.08-2.07 (m, 1H), 1.98-1.96 (m, 4H), 1.75-1.72 (m, 4H), 1.65-1.64 (m, 1H), 1.57-1.54 (m, 2H) and 0.88 (m, 2H). LCMS: [M+H] ⁺ : 559.48, Purity= 91.82%.

Example B0700-54

Synthesis of Compound B0700-603:

Preparation of (R)-N-(l-((2-(N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)sulfamoyl)pyridin-4-yl)methyl)piperidin-3-yl)acryl amide (B0700-603):

Synthesis of 2-(benzylthio)-4-bromopyridine (263):

[00846] To an ice cold sodium hydride (2.27 g, 56.8 mmol) in tetrahydrofuran (235 mL) was added phenylmethanethiol 262 (7.06 g, 56.8 mmol) drop wise at 0°C under N2 atmosphere. The reaction mixture was allowed to stirred at 0°C for 2h. Then added 4-bromo-2-fluoropyridine 261 (10 g, 56.8 mmol) dissolved in tetrahydrofuran (235 mL) drop wise to the reaction mixture at 0°C and allowed to stirred at same temperature for 2 h. After TLC monitoring the reaction mixture was diluted with water (250 mL) and extracted with ethyl acetate (2 x 200 mL). The organic layer was dried over anhydrous ISfeSCL, filtered and concentrated under reduced pressure to get crude. Crude was loaded into combi flash using silica gel (230-400 M) and eluted in 1% EtOAc in Heptane to get desired product as transparent oily compound 263 (12.6 g, 57.77%).

^X HNMR (400 MHz, CDCh): 8 8.26-8.25 (d, J= 4.4 Hz, 1H), 7.40-7.38 (d, J= 8.04 Hz, 2H), 7.34-7.24 (m, 4H), 7.15-7.14 (d, J= 4.02 Hz, 1H) and 4.42 (s, 2H). LCMS: [M+H] ⁺ : 280.02, Purity= 96.93%.

Synthesis of 4-bromopyridine-2-sulfonyl chloride (264):

[00847] To an ice cold stirred solution of 2-(benzylsulfanyl)-4-bromopyridine 263 (2.2 g, 7.85 mmol) in dichloromethane (31 mL), acetic acid (4.4 mL) and water (8.8 mL) was added 1,3- dichloro-5,5-dimethylimidazolidine-2, 4-dione (4.64 g, 23.6 mmol) dissolved in DCM (10 mL), resulting reaction mixture was stirred at room temperature for next 16h. After reaction monitoring (TLC), reaction mass was quenched with brine water (50 mL) and extracted with DCM (2 x 100 mL). The combined organic layer was washed with aq NaHCOs (50 mL) and DM water (50 mL) sequentially, organic layer was dried over ISfeSCU, filtered and concentrated under reduced pressure below 40°C in water bath to get crude. The crude was triturated with Diethyl ether, filtered the solid material and filtrate was concentrated under reduced pressure to get milky viscous liquid desired product 264 (3 g). The crude product used as such without purification for next reaction.

Synthesis of 4-bromo-N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)meth yl)-7H-pyrrolo [2,3- d]pyrimidin-6-yl)phenyl)pyridine-2-sulfonamide (265):

[00848] To an ice cold stirred solution of 4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 7 (2.5 g, 5.87 mmol) in dichloromethane (44.4 mL) were added pyridine (2.37 mL, 29.4 mmol) and 4-bromopyridine-2- sulfonyl chloride 264 (2.26 g, 8.81 mmol) dissolved in DCM (4 mL) in dropwise at 0°C, the resulting reaction mixture was stirred at room temperature for next Ih. After completion of reaction (TLC monitoring), quenched with water (50 mL) and extracted with DCM (2x50 mL). The combined organic layer was dried over sodium sulphate, filtered and concentrated under reduced pressure to get red crude. The crude residue was purified by column chromatography in silica gel (230-400M) using eluents 40-50% EtOAc in heptane to get red viscous solid 265 (1.75 g, 36.91%). 'H NMR (400 MHz, DMSO-d6): 5 10.90 (s, 1H), 8.63-8.61 (d, J= 8.06 Hz, 1H), 8.31 (s, 1H), 8.24- 8.20 (m, 2H), 7.99-7.93 (m, 2H), 7.64-7.62 (d, J= 8.0 Hz, 2H), 7.26-7.24 (d, 2H), 6.87 (s, 1H), 5.49 (s, 2H), 3.86 (s, 4H), 3.71 (s, 4H), 0.83-0.81 (m, 2H) and -0.12 (s, 9H). LCMS: [M+H] ⁺ : 645.08, Purity= 81%.

Synthesis of N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-p yrrolo [2,3- d]pyrimidin-6-yl)phenyl)-4-vinylpyridine-2-sulfonamide (266):

[00849] To a stirred solution of 4-bromo-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}pyridine-2-sulfonamide

265 (1.25 g, 1.94 mmol) and 2-ethenyl-4,4,5,5-tetramethyl-l,3,2-dioxaborolane 243 (895 mg, 5.81 mmol) in 1,4-dioxane (20 mL) was added NazCOs (616 mg, 5.81 mmol) dissolved in water (5 mL) under nitrogen atmosphere. The resulting reaction mixture was degassed with argon gas for 10 min.

Then added l,r-Bis(diphenylphosphino)ferrocene]dichloropalladium(II) (142 mg, 194 pmol) to the reaction mixture. The reaction mixture was stirred at 80°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (2 x 100 mL). The combined organic layer was washed with brine solution (100 mL), dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. The crude was purified by column chromatography silica gel (40 g SNAP) eluted with 20-25% EtOAc in heptane to get desired product as brown oily compound 266 (0.8 g, 69.71%).

^X H NMR (400 MHz, DMSO-d6): 5 10.53 (s, 1H), 8.68-8.66 (d, J= 8.4 Hz, 1H), 8.24 (s, 1H), 8.08 (s, 1H), 7.75-7.74 (d, J= 4.0 Hz, 1H), 7.62-7.60 (d, J= 8.20 Hz, 2H), 7.26-7.24 (m, 2H), 6.85 (m, 2H), 6.29-6.25 (m, 1H), 5.67-5.65 (m, 1H), 5.48 (s, 2H), 3.85 (s, 4H), 3.70 (s, 4H), 3.56-3.52 (m, 2H), 0.82- 0.80 (m, 2H) and -0.12 (s, 9H). LCMS: [M+H] ⁺ : 593.26, Purity= 87.52%.

Synthesis of 4-formyl-N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)met hyl)-7H-pyrrolo [2,3- d]pyrimidin-6-yl)phenyl)pyridine-2-sulfonamide ( 267):

[00850] To a stirred solution of 4-ethenyl-N-{4-[4-(morpholin-4-yl)-7-{[2-

(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin -6-yl]phenyl}pyridine-2-sulfonamide

266 (0.6 g, 1.01 mmol) in THF (30 mL) and water (15 mL) was added OsO4 (643 mg, 101 pmol), stirred at room temperature for next 5h. Then sodium periodate (649 mg, 3.04 mmol) in water (10 mL) was added at same temperature and stirred at room temperature for next 16h. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (2x 100 mL), dried over Na2SO4, filtered and concentrated under reduced pressure to get crude residue (0.62 g, 99%). The crude was used without purification for next reaction.

Synthesis of tert-butyl (R)-(l-((2-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy) methyl)-7H- pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)sulfamoyl)pyridin-4-yl)methyl)piperidi n-3-yl)carbamate ( 268):

[00851] To a stirred solution of 4-formyl-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}pyridine-2-sulfonamide 267 (620 mg, 1.04 mmol) and tert-butyl N-[(3R)-piperidin-3-yl]carbamate 18 (313 mg, 1.56 mmol) in 1,2-di chloroethane (10 mL) were added sodium acetate (171 mg, 2.08 mmol) and sodium bis(acetyloxy)boranuidyl acetate (442 mg, 2.08 mmol), stirred reaction mixture at room temperature for next 16h. After completion of reaction (TLC monitoring), the reaction mixture was poured into water (50 mL) and extracted with DCM (3 x 50 mL). The combined organic layer was washed with brine solution (50 mL), dried over Na2SO4, filtered and concentrated under reduced pressure to get crude residue, which was purified over silica gel (230-400M) column chromatography using eluent 35% ethyl acetate in heptane to get the desired product as brown solid 268 (370 mg, 45%).

^X H NMR (400 MHz, DMSO-d6): 5 10.74 (s, 1H), 8.63-8.61 (d, J= 8.02 Hz, 1H), 8.24 (s, 1H), 7.95 (s, 1H), 7.61-7.58 (m, 3H), 7.22-7.20 (d, J= 8.20 Hz, 2H), 6.82 (s, 1H), 6.71 (d, 1H), 5.48 (s, 2H), 3.85 (s, 4H), 3.71-3.55 (m, 9H), 2.70 (m, 1H), 1.98-1.96 (m, 2H), 1.85-1.84 (m, 1H), 1.57-1.55 (m, 1H), 1.35 (m, 1H), 1.33 (s, 9H), 1.28 (m, 1H) 1.16-1.14 (m, 1H), 0.81 (m, 2H), and -0.115 (s, 9H). LCMS: [M+H] ⁺ : 779.31, Purity= 82.20%.

Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-7H- pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)pyridine-2-sulfonamide ( 269):

[00852] To an ice cold solution of tert-butyl N-[(3R)-l-{[2-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}sulfamoyl)pyri din-4- yl]methyl}piperi din-3 -yl]carbamate 268 (350 mg, 449 pmol) in di chloromethane (6 mL) was added trifluoroacetic acid (3 mL) and, reaction mixture was stirred at room temperature for next 2h. After completion of SM (TLC monitoring), SM was fully consumed, reaction mixture was concentrated under reduced pressure to get crude. Crude was dissolved in 1,4-di oxane (5 mL) was added ethane- 1,2-diamine (81 mg, 1.35 mmol), heated upto 80°C for next 2h. After completion of reaction (TLC monitoring), concentrated under reduced pressure to get crude. Crude was dissolved in 20% IPA in CHCh and washed with brine solution, organic layer was dried over Na2SO4, filtered and concentrated under reduced pressured to get desired product as off white solid 269 (250 mg). MS: [M+H] ⁺ : 549.17.

Synthesis of (R)-N-(l-((2-(N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)sulfamoyl)pyridin-4-yl)methyl)piperidin-3-yl)acryl amide (B0700-603):

[00853] To an ice cold stirred solution of 4-{[(3R)-3-aminopiperidin-l-yl]methyl}-N-{4-[4- (morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}pyri dine-2-sulfonamide 269 (250 mg, 456 pmol) in dimethylformamide (3 mL) was added triethylamine (192 pL, 1.37 mmol) and prop-2 - enoyl chloride 27 (20.6 mg, 228 pmol). The resulting reaction mass was stirred at same temperature for next 30 minutes. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated up to dryness and the crude residue was purified through RP-HPLC purification eluted with 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column: Waters Xselect Phenyl- Hexyl(19*250mm,5pm) to get desired product as off white solid B0700-603 (24 mg, 9%).

¹ H NMR (400 MHz, DMSO-d6): 5 12.09 (s, 1H), 10.76 (s, 1H), 8.63-8.62 (d, J= 4.4 Hz, 1H), 8.15 (s, 1H), 7.96-7.92 (m, 2H), 7.73-7.71 (m, 2H), 7.55-7.54 (d, J= 4.0 Hz, 1H), 7.16-7.14 (d, J= 8.02 Hz, 2H), 7.01 (s, 1H), 6.24-6.18 (m, 1H), 6.08-6.03 (m, 1H), 5.56-5.53 (dd, 1H), 3.84-3.83 (d, 5H), 3.73- 3.72 (d, 4H), 3.66-3.57 (m, 1H), 2.75 (m, 1H), 2.43 (m, 2H), 1.94-1.88 (m, 2H), 1.71-1.68 (m, 2H), 1.43-1.39 (m, 1H) and 1.10 (m, 1H). LCMS: [M+H] ⁺ : 603.36, Purity= 97.50%.

Example B0700-55

Synthesis of Compound B0700-604

Preparation of N-((3R)-l-(3-(l-(N-(3-fluoro-4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)sulfamoyl)ethyl)phenyl)piperidin-3-yl)acrylamide (B0700-604):

Synthesis of l-(3-bromophenyl)-N-(3-fluoro-4-(4-morpholino-7-((2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)methanesulfonamide (270):

[00854] To a stirred solution of 3-fluoro-4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 202 (1.4 g, 3.16 mmol) in dichloromethane (28 mL) was added ethylbi s(propan-2-yl)amine (2.76 mL, 15.8 mmol) and 4- (dimethylamino)pyridin-l-ium (38.9 mg, 316 pmol) at RT under N2 atmosphere. The reaction mixture was stirred for 15 min. Then (3-bromophenyl)methanesulfonyl chloride 153 (2.55 g, 9.47 mmol) was added in fractions to the reaction mixture for a period of Ih. The reaction mixture was stirred for 16h at RT. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (50 mL) and extracted with DCM (2 x 50 mL). The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 22% ethyl acetate in heptane to get the desired product as white-solid 270 (1 g, 35%). ^X H NMR (400 MHz, DMSO-d6): 8 8.31 (s, 1H), 7.59-7.57 (d, J= 8.0 Hz, 2H), 7.50 (s, 1H), 7.33-7.30 (m, 3H), 7.10-7.07 (m, 2H), 6.85 (s, 1H), 5.48 (s, 2H), 4.65 (s, 2H), 3.87 (s, 4H), 3.72 (s, 4H), 3.41- 3.37 (t, 2H), 0.76-0.72 (m, 2H) and -0.11 (s, 9H), LCMS: [M+H] ⁺ : 676.26, Purity= 73%.

Synthesis of l-(3-bromophenyl)-N-(3-fluoro-4-(4-morpholino-7-((2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)-N- (methoxymethyl)methanesulfonamide (271):

[00855] To an ice-cold stirred solution of l-(3-bromophenyl)-N-{3-fluoro-4-[4-(morpholin-4-yl)-7- {[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidi n-6-yl]phenyl}methanesulfonamide 270 (1 g, 1.48 mmol) in dichloromethane (20 mL) was added DIPEA (774 pL, 4.43 mmol) under N2 atmosphere. The reaction mixture was stirred for 15 min. Then added chloro(methoxy)methane (224 pL, 2.96 mmol) to the reaction mixture. The reaction mixture was stirred for Ih at RT. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (50 mL) and extracted with ethyl acetate (2 x 50 mL). The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. The crude residue was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 15-20% ethyl acetate in heptane to get the desired product as white-solid 271 (800 mg, 35%).

'H NMR (400 MHz, DMSO-d6): 5 8.29 (s, 1H), 7.70-7.68 (d, J= 8.04 Hz, 2H), 7.62 (s, IH), 7.60- 7.58 (m, 2H), 7.43-7.42 (d, J= 4.2 Hz, IH), 7.37-7.35 (m, 3H), 6.95 (s, IH), 5.75 (s, 2H), 4.97 (s, 2H), 4.70 (s, 2H), 3.88 (s, 4H), 3.72 (s, 4H), 3.42 (s, 5H), 0.87-0.85 (m, 2H) and -0.13 (s, 9H). LCMS: [M+H]’: 720.16, Purity= 80%.

Synthesis of l-(3-bromophenyl)-N-(3-fluoro-4-(4-morpholino-7-((2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)-N- (methoxymethyl)ethane-l-sulfonamide (272):

[00856] To an ice cold stirred solution of l-(3-bromophenyl)-N-{3-fluoro-4-[4-(morpholin-4-yl)-7- {[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidi n-6-yl]phenyl}-N- (methoxymethyl)methanesulfonamide 271 (0.8 g, 1.11 mmol) in tetrahydrofuran (9.0 mL) was added lithium(l+) bis(trimethylsilyl)azanide (1.66 mL, 1.66 mmol) dropwise at -78°C under N2 atmosphere. The reaction mixture was stirred for Ih. Then added stock solution of iodomethane (69.1 pL, 1.11 mmol) dissolved in tetrahydrofuran (0.6 mL) to the reaction mixture. The reaction mixture was stirred for another Ih at same temperature. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (25 mL) and extracted with ethyl acetate (2 x 50 mL). The organic layer was dried over ISfeSCU, filtered and concentrated under reduced pressure to get crude. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) using eluents 20% ethyl acetate in heptane to get the desired product as white-solid 272 (378 mg, 37%).

LCMS: [M+H]': 734.27, Purity= 81%.

Synthesis of tert-butyl ((3R)-l-(3-(l-(N-(3-fluoro-4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-N- (methoxymethyl)sulfamoyl)ethyl)phenyl)piperidin-3-yl)carbama te (273):

[00857] To a stirred solution of l-(3-bromophenyl)-N-{3-fluoro-4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-N-

(methoxymethyl)ethane-l -sulfonamide 272 (370 mg, 504 pmol) and tert-butyl N-[(3R)-piperidin-3- yl]carbamate 18 (202 mg, 1.01 mmol) in toluene (7 mL) was added CS2CO3 (492 mg, 1.51 mmol). The reaction mixture was degassed with argon gas for 5 min. Then added tris((lE,4E)-l,5- diphenylpenta-l,4-dien-3-one) palladium (40.8 mg, 50.4 pmol) and [2',6'-bis(propan-2-yloxy)-[l,l'- biphenyl]-2-yl]dicyclohexylphosphane (47 mg, 101 pmol) to the reaction mixture. The reaction mixture was stirred at 100°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (3 x 20 mL). The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) using eluents 24% ethyl acetate in heptane to get the desired product as white-solid 273 (350 mg, 60%).

LCMS: [M+H]': 854, Purity= 81%.

Synthesis of l-(3-((R)-3-aminopiperidin-l-yl)phenyl)-N-(3-fluoro-4-(4-mor pholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)ethane-l-sulfonamide (274):

To an ice-cold stirred solution of tert-butyl N-[(3R)-l-{3-[l-({3-fluoro-4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}(methoxymethyl)sulfamoyl)ethyl]phenyl}piperidin-3- yl]carbamate 273 (350 mg, 410 pmol) in ethyl acetate (5 mL) was added hydrogen chloride (137 pL, 1.64 mmol) dropwise under N2 atmosphere. The reaction mixture was stirred at RT for 6h. After completion of reaction (LCMS monitoring) the reaction mixture was basified with aq. NaHCCh and extracted with 10% IPA in CHCh. The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get desired product as white solid 274 (285 mg, 74%).

MS: [M+H] ⁺ : 710.26.

Synthesis of l-(3-((R)-3-aminopiperidin-l-yl)phenyl)-N-(3-fluoro-4-(4-mor pholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)ethane-l-sulfonamide (275):

[00858] To an ice-cold stirred solution of l-{3-[(3R)-3-aminopiperidin-l-yl]phenyl}-N-{3-fluoro-4- [4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H- pyrrolo[2,3-d]pyrimidin-6- yl]phenyl} ethane- 1 -sulfonamide 274 (275 mg, 387 pmol) in dichloromethane (6 mL) was added trifluoroacetic acid (2 mL) dropwise under N2 atmosphere. The reaction mixture was stirred for 3h at RT. After completion of reaction (LCMS monitoring), the reaction mixture was concentrated under reduced pressure to get crude. The crude was basified using aq. NaHCCh and extracted with 10% IPA in CHCh (2x 100 mL). The combined organic layer was dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get the desired product as brown solid 275 (190 mg, 85%).

MS: [M+H] ⁺ : 580.42.

Synthesis of N-((3R)-l-(3-(l-(N-(3-fluoro-4-(4-morpholino-7H-pyrrolo[2,3- d]pyrimidin-6- yl)phenyl)sulfamoyl)ethyl)phenyl)piperidin-3-yl)acrylamide (B0700-604):

[00859] To an ice-cold stirred solution of l-{3-[(3R)-3-aminopiperidin-l-yl]phenyl}-N-{3-fluoro-4- [4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl} ethane- 1 -sulfonamide 275 (180 mg, 311 pmol) in dimethylformamide (1.5 mL) was added triethylamine (129 pL, 932 pmol) under N2 atmosphere. The reaction mixture was stirred for 15 min. Then added stock solution of prop-2-enoyl chloride 27 (33.7 mg, 373 pmol) in dimethylformamide (0.5 mL) dropwise to the reaction mixture. The reaction mixture was stirred for Ih. After completion of reaction (LCMS monitoring) the reaction mixture was poured into ice-cold water (100 mL) and extracted with 10% IPA in CHCh (50 mL). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to get crude. The crude residue was purified through RP-HPLC purification using eluent as 5mM ammonium bicarbonate in water/acetonitrile and column using Waters Xselect Phenyl- Hexyl(19*250mm, 5pm to get desired product as pale yellow solid B0700-604 (4 mg, 2%).

^X H NMR (400 MHz, DMSO-d6): 5 12.11 (s, IH), 10.09 (s, IH), 8.20 (s, IH), 8.09-8.07 (d, J= 8.40 Hz, IH), 7.78-7.73 (t, IH), 7.17-7.13 (t, IH), 6.94-6.16 (m, 6H), 6.27-6.27 (m, IH), 6.12-6.08 (m, 1H), 5.58-5.54 (dd, 1H), 4.50 (s, 1H), 3.86-3.85 (d, 4H), 3.80 (m, 1H), 3.75-3.74 (d, 4H), 3.56-3.53 (m, 1H), 2.75 (m, 1H), 2.43 (m, 1H), 1.85-1.81 (m, 1H), 1.72-1.69 (m, 1H), 1.65-1.64 (d, 3H), 1.53- 1.50 (m, 1H), 1.41-1.38 (m, 1H), 1.11 (m, 1H) and 0.87 (m, 1H). LCMS: [M+H] ⁺ : 634.39, Purity= 99.29%.

Example B0700-56

Synthesis of Compound B0700-605

Preparation of N-((3R)-l-(3-(l-(N-(2-fluoro-4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)sulfamoyl)ethyl)phenyl)pyrrolidin-3-yl)acrylamide (B0700-605):

Synthesis of tert-butyl ((3R)-l-(3-(l-(N-(2-fluoro-4-(4-morpholino-7-((2-(trimethyls ilyl)ethoxy) methyl)-7H-pyrrolo[2,3-d] pyrimidin-6-yl)phenyl)-N-(methoxymethyl) sulfamoyl)ethyl)phenyl)pyrrolidin-3-yl) carbamate (276):

[00860] To a stirred solution of l-(3-bromophenyl)-N-{2-fluoro-4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-N- (methoxymethyl)ethane-l -sulfonamide 232 (0.4 g, 544 pmol) and tert-butyl N-[(3R)-pyrrolidin-3- yl]carbamate 205 (203 mg, 1.09 mmol) in toluene (16 mL) was added CS2CO3 (532 mg, 1.63 mmol), the resulting mixture was purged with argon for 10 min. After addition of tris(l,5-diphenylpenta-l,4- dien-3-one) dipalladium (49.9 mg, 54.4 pmol) and [2',6'-bis(propan-2-yloxy)-[l,l'-biphenyl]-2- yl]dicyclohexylphosphane (50.8 mg, 109 pmol), reaction mixture was heated at 100°C for next 16h. After reaction monitoring (TLC monitoring), the reaction mixture quenched with DM water (50 mL) and extracted with EtOAc (2x50 mL). Organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get yellow crude. Crude was purified by flash chromatography in silica using 30% EtOAc in heptane to get desired product as light brown solid 276 (310 mg, 67.78%). LCMS: [M+H] ⁺ : 840.34, Purity= 92.29%.

Synthesis of l-(3-((R)-3-aminopyrrolidin-l-yl)phenyl)-N-(2-fluoro-4-(4-mo rpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)ethane-l-sulfonamide (277):

[00861] To an ice-cold stirred solution of tert-butyl N-[(3R)-l-{3-[l-({2-fluoro-4-[4-(morpholin-4- yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]py rimidin-6- yl]phenyl}(methoxymethyl)sulfamoyl)ethyl]phenyl}pyrrolidin-3 -yl]carbamate 276 (310 mg, 369 pmol) in ethyl acetate (4 mL) was added hydrogen chloride (0.07 mL) dropwise under N2 atmosphere. The reaction mixture was stirred at RT for 5 h. After completion of reaction (LCMS monitoring), the reaction mixture was concentrated under reduced pressure to get crude. The crude was cooled to 0°C, basified with aq. NaHCOs and extracted with 10% IP A in CHCL (2 x 100 mL). The combined organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get desired product as white solid 277 (280 mg, 64%). MS: [M+H]+ : 696.41.

Synthesis of l-(3-((R)-3-aminopyrrolidin-l-yl)phenyl)-N-(2-fluoro-4-(4-mo rpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)ethane-l-sulfonamide (278):

[00862] To an ice cold stirred solution of l-{3-[(3R)-3-aminopyrrolidin-l-yl]phenyl}-N-{2-fluoro-4- [4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H- pyrrolo[2,3-d]pyrimidin-6- yl]phenyl} ethane- 1 -sulfonamide 277 (280 mg, 402 pmol) in di chloromethane (5 mL) was added trifluoroacetic acid (2.5 mL) at 0°C, stirred for at room temperature for next 2h. Reaction mixture was concentrated under reduced pressure to get crude. Crude residue was dissolved in 1,4-di oxane (5 mL) was added ethane- 1,2-diamine (72.5 mg, 1.21 mmol), after reaction monitoring (LCMS), concentrated under reduced pressured. The crude was dissolved in 20% IP A in chloroform (100 mL) and washed with water (50 mL). Organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get desired product as brown solid 278 (200 mg, 88%).

MS: [M+H]+ : 566.25. Synthesis of N-((3R)-l-(3-(l-(N-(2-fluoro-4-(4-morpholino-7H-pyrrolo[2,3- d]pyrimidin-6- yl)phenyl)sulfamoyl)ethyl)phenyl)pyrrolidin-3-yl)acrylamide (B0700-603):

[00863] To an ice-cold stirred solution of l-{3-[(3R)-3-aminopyrrolidin-l-yl]phenyl}-N-{2-fluoro- 4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl }ethane-l-sulfonamide 278 (0.2 g, 354 pmol) in N,N-dimethylformamide (3 mL) was added triethylamine (149 pL, 1.06 mmol) drop wise under N2 atmosphere. The reaction mixture was stirred for 30 min at same temperature. Then added stock solution of prop-2-enoyl chloride 27 (32 mg, 354 pmol) in dimethylformamide (0.3 mL) drop wise to the reaction mixture. The reaction mixture was stirred for next 15min at same temperature. After completion of reaction (TLC and LCMS monitoring) the reaction mixture was poured into ice-cold water and extracted with 10% IPA in CHC13 (2x 100 mL). The organic layer was washed with chilled brine solution (50 mL), dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. The crude residue was purified through RP-HPLC to get desired product aa off white solid B0700-603 (4 mg, 2%).

¹ H NMR (400 MHz, DMSO-d6): 5 12.24 (s, 1H), 9.67 (s, 1H), 8.38-8.36 (d, J= 8.06 Hz, 1H), 8.18 (s, 1H), 7.81-7.79 (d, J= 8.06 Hz, 1H), 7.65-7.63 (d, J= 8.06 Hz 1H), 7.33-7.28 (m, 1H), 7.25 (s, 1H), 7.13-7.09 (t, 1H), 6.65-6.63 (m, 1H), 6.51 (s, 1H), 6.47-6.45 (m, 1H), 6.25-6.18 (m, 1H), 6.12-6.07 (m, 1H), 5.59-5.56 (m, 1H), 4.43-4.36 (m, 2H), 3.88-3.87 (d, 4H), 3.75-3.74 (d, 4H), 3.46-3.44 (m, 1H), 3.32 (m, 1H), 3.22-3.19 (m, 1H), 3.07-3.06 (m, 1H), 2.18-2.14 (m, 1H), 1.91-1.89 (m, 1H) and 1.67-1.65 (d, 3H). LCMS: [M+H]+: 620.34, Purity= 96.27%.

Example B0700-57

Synthesis of Compound B0700-606:

Preparation of N-((3R)-l-(3-(l-(N-(3-fluoro-4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)sulfamoyl)ethyl)phenyl)pyrrolidin-3-yl)acrylamide (B0700-606): Synthesis of tert-butyl ((3R)-l-(3-(l-(N-(3-fluoro-4-(4-morpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-N- (methoxymethyl)sulfamoyl)ethyl)phenyl)pyrrolidin-3-yl)carbam ate (279):

[00864] To a stirred solution of l-(3-bromophenyl)-N-{3-fluoro-4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-N- (methoxymethyl)ethane-l -sulfonamide 272 (578 mg, 787 pmol) and tert-butyl N-[(3R)-pyrrolidin-3- yl]carbamate 205 (293 mg, 1.57 mmol) in toluene (6 mL) was added CS2CO3 (769 mg, 2.36 mmol) at RT. The reaction mixture was degassed with argon gas for 5 min. Then added tris((lE,4E)-l,5- diphenylpenta-l,4-dien-3-one) palladium (72 mg, 78.7 pmol) and [2',6'-bis(propan-2-yloxy)-[l,l'- biphenyl]-2-yl]dicyclohexylphosphane (73.4 mg, 157 pmol) to the reaction mixture. The reaction mixture was stirred at 100°C for 2h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (50 mL) and extracted with ethyl acetate (2 x 50 mL). The organic layer was dried over ISfeSCU, filtered and concentrated under reduced pressure to get crude. The crude residue was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 17% ethyl acetate in heptane to get the desired product as white-solid 279 (480 mg, 49%).

LCMS: [M+H] ⁺ : 840.33, Purity= 67%.

Synthesis of l-(3-((R)-3-aminopyrrolidin-l-yl)phenyl)-N-(3-fluoro-4-(4-mo rpholino-7-((2- (trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)ethane-l-sulfonamide (280):

[00865] To an ice-cold stirred solution of tert-butyl N-[(3R)-l-{3-[l-({3-fhioro-4-[4-(morpholin-4- yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]py rimidin-6- yl]phenyl}(methoxymethyl)sulfamoyl)ethyl]phenyl}pyrrolidin-3 -yl]carbamate 279 (450 mg, 536 pmol) in ethyl acetate (6 mL) was added hydrogen chloride (78.1 mg, 2.14 mmol) dropwise under N2 atmosphere. The reaction mixture was stirred at RT for 5 h. After completion of reaction (LCMS monitoring), the reaction mixture was concentrated under reduced pressure to get crude. Crude was cooled to 0°C, basified with aq. NaHCOs and extracted with 10% IPA in CHCL (2x100 mL). The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get desired product as white solid 280 (370 mg, 64%).

MS: [M+H] ⁺ : 696.51. Synthesis of l-(3-((R)-3-aminopyrrolidin-l-yl)phenyl)-N-(3-fluoro-4-(4-mo rpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)ethane-l-sulfonamide (281):

[00866] To an ice-cold stirred solution of l-{3-[(3R)-3-aminopyrrolidin-l-yl]phenyl}-N-{3-fluoro- 4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7 H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl} ethane- 1 -sulfonamide 280 (370 mg, 545 pmol) in dichloromethane (5 mL) was added trifluoroacetic acid (1.5 mL) dropwise under N2 atmosphere. The reaction mixture was stirred at RT for 5h. After LCMS monitoring the reaction mixture was concentrated under reduced pressure to get crude. Crude was then dissolved in 1,4-dioxane (5 mL) was added ethane- 1,2-diamine (49.1 mg, 817 pmol) dropwise at 0°C. The reaction mixture was stirred at 70°C for Ih. The reaction mixture was then concentrated under reduced pressure to get crude. Crude was then diluted with water (50 mL) and extracted with 10% IP A in CHCL (2x 100 mL). The combined organic layer was dried over Na2SC>4, filtered and concentrated under reduced pressure to get the desired product as brown solid 281 (180 mg, 31%).

MS: [M+H] ⁺ : 566.22.

Synthesis of N-((3R)-l-(3-(l-(N-(3-fluoro-4-(4-morpholino-7H-pyrrolo[2,3- d]pyrimidin-6- yl)phenyl)sulfamoyl)ethyl)phenyl)pyrrolidin-3-yl)acrylamide (B0700-606):

[00867] To an ice-cold stirred solution of l-{3-[(3R)-3-aminopyrrolidin-l-yl]phenyl}-N-{3-fluoro- 4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl }ethane-l-sulfonamide 281 (180 mg, 318 pmol) in dimethylformamide (1.2 mL) was added triethylamine (132 pL, 955 pmol) dropwise under N2 atmosphere. The reaction mixture was stirred for 30 min at same temperature. Then added stock solution of prop-2-enoyl chloride 27 (20.2 mg, 223 pmol) in dimethylformamide (0.3 mL) dropwise to the reaction mixture. The reaction mixture was stirred for next 15min at same temperature. After completion of reaction (TLC and LCMS monitoring) the reaction mixture was poured into ice-cold water and extracted with 10% IP A in CHCL. The organic layer was washed with chilled brine solution, dried over ISfeSCU, filtered and concentrated under reduced pressure to get crude. The crude residue was purified through RP-HPLC purification using eluent as 5mM ammonium bicarbonate in water/acetonitrile and column using Waters Xselect Phenyl-Hexyl (19*250mm, 5pm to get desired product as pale yellow solid B0700-606 (10 mg, 5%).

'H NMR (400 MHz, DMSO-d6): 5 12.13 (s, IH), 10.03 (s, 1H), 8.37-8.35 (d, J= 8.40 Hz IH), 8.20 (s, IH), 7.78-7.74 (d, J= 8.40 Hz IH), 7.14-7.10 (t, IH), 7.00-6.91 (m, 3H), 6.62-6.60 (d, IH), 6.48- 6.66 (t, 2H), 6.25-6.19 (m, IH), 6.13-6.07 (m, IH), 5.59-5.54 (m, IH), 4.47-4.38 (m, 2H), 3.86-3.85 (d, 4H), 3.75-3.74 (d, 4H), 3.41-3.40 (m, 1H), 3.32 (m, 1H), 3.17-3.14 (m, 1H), 3.03-3.01 (m, 1H), 2.19-2.13 (m, 1H), 1.90-1.85 (m, 1H) and 1.65-1.63 (d, 3 H). LCMS: [M+H] ⁺ : 620.34, Purity= 97.30%.

Example B0700-58

Synthesis of Compound B0700-607: Preparation of 4-(((l S,3R)-3-acrylamidocyclohexyl)amino)-N-(4-(4-morpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)picolinamide (B0700-607):

Synthesis of tert-butyl ((lR,3S)-3-((2-cyanopyridin-4-yl)amino)cyclohexyl)carbamate (284): [00868] To a stirred ice cold solution of tert-butyl N-[(lS,3R)-3-aminocyclohexyl]carbamate 282 (0.5 g, 2.33 mmol) and 4-fluoropyridine-2-carbonitrile 283 (427 mg, 3.5 mmol) in 1- methylpyrrolidin-2-one (5 mL) was added DIPEA (1.20 mL, 7 mmol) at same temperature. The resulting reaction mixture was stirred at 140°C for 16h. After the completion of reaction (TLC monitoring) The reaction mixture was diluted with ice water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (2 x 100 mL), dried over Na2SO4, filtered and evaporated under reduced pressure. The crude was purified by silica- gel flash column chromatography (50% EtOAc/Heptane) to get desired product as yellow solid 284 (0.6 g, 65%).

'H NMR (400 MHz, DMSO-d6): 8 8.07-8.05 (d, 1H), 7.05 (s, 1H), 7.01-7.00 (d, J= 4.4 Hz 1H), 6.78 (s, 1H), 6.71-6.70 (d, J= 4.2 Hz 1H), 3.31-3.28 (m, 1H), 2.19-2.15 (t, 1H), 1.98-1.67 (m, 5H), 1.36 (s, 9H) and 1.08-1.01 (m, 4H). LCMS: [M+H] ⁺ : 317.30, Purity= 92.05%. Synthesis of 4-(((lS,3R)-3-((tert-butoxycarbonyl)amino)cyclohexyl)amino)p icolinic acid (285): [00869] To a stirred solution of tert-butyl N-[(lR,3S)-3-[(2-cyanopyridin-4- yl)amino]cyclohexyl]carbamate 284 (543 mg, 1.72 mmol) in 1,4-dioxane (5 mL) was added LiOH.FEO (288 mg, 6.87 mmol) in water (2.5 mL), The reaction mixture was stirred at 80°C for next 4h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressured. Crude was dissolved in 20% IPA in CHCh (100 mL) and filtered through sintered. Filtrate was concentrated under reduced pressure to get desired product as off white solid 285 (560 mg, as Li salt, quantification not done ).

LCMS: [M+H] ⁺ : 338.28, Purity= 96.68%.

Synthesis of tert-butyl ((lR,3S)-3-((2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethox y)methyl)- 7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-y l)amino)cyclohexyl)carbamate (286):

[00870] To a stirred solution of 4-{[(lS,3R)-3-{[(tert- butoxy)carbonyl]amino}cyclohexyl]amino}pyridine-2-carboxylic acid 285 (650 mg, 1.94 mmol) ,4- [4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H- pyrrolo[2,3-d]pyrimidin-6-yl]aniline 7 (660 mg, 1.55 mmol) in N,N-dimethylformamide (10 mL) where added DIPEA (1.0 mL, 5.81 mmol) and add HATU (1.11 g, 2.91 mmol) in to the reaction mass, reaction mass was stirred at RT for next 16h. After completion of reaction (TLC monitoring), the reaction mass was poured into ice- cold water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (2 x 50 mL), dried over ISfeSCU, filtered and evaporated under reduced pressure. The crude was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 50% ethyl acetate in heptane to get the desired product as brown solid 286 (950 mg, 57.4%). LCMS: [M+H] ⁺ : 743.65, Purity= 87.08%.

Synthesis of 4-(((lS,3R)-3-aminocyclohexyl)amino)-N-(4-(4-morpholino-7H-p yrrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide (287):

[00871] To an ice cold stirred solution of tert-butyl N-[(lR,3S)-3-{[2-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)pyridin-4- yl]amino}cyclohexyl]carbamate 286 (0.9 g, 1.21 mmol) in dichloromethane (10 mL) was added trifluoroacetic acid (5 mL) dropwise under N2 atmosphere. The resulting reaction mixture was stirred at RT for next 2h. After completion of reaction (LCMS monitoring), the reaction mixture was concentrated under reduced pressure. The crude was dissolved in 1,4-di oxane (10 mL) followed by addition of ethane- 1,2-diamine (218 mg, 3.63 mmol) at 0°C and the reaction mixture was stirred at 80°C for 2h. After completion of reaction (LCMS monitoring), the reaction mixture was concentrated under reduced pressure. The crude was diluted with water (100 mL) and extracted with 25% IPA in Chloroform (3X 100 mL). Combined organic layer was dried over ISfeSCU, filtered and concentrated under reduced pressure to get desired product as brown viscous liquid 287 (320 mg, 51%).

MS: [M+H] ⁺ : 513.60.

Synthesis of 4-(((lS,3R)-3-acrylamidocyclohexyl)amino)-N-(4-(4-morpholino -7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide (B0700-607):

[00872] To an ice cold stirred solution of 4-{[(lS,3R)-3-aminocyclohexyl]amino}-N-{4-[4- (morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}pyri dine-2-carboxamide 287 (0.3 g, 585 pmol) in N,N-dimethylformamide (3.5 mL) were added triethylamine (0.32 mL, 2.34 mmol) and prop-2-enoyl chloride 7 (53 mg, 585 pmol) in drop wise manner. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS monitoring), the reaction mixture was poured into ice cold water (50 mL) and extracted with 25% IPA/Chloroform (3 X 100 mL). Combined organic layer was dried over NajSCh, filtered through cotton plug. Filtrate was concentrate under reduced pressure. The crude residue was purified through RP-HPLC purification to get desired product as yellow solid B0700-607 (53 mg, 16%).

'H NMR (400 MHz, DMSO-d6): 5 12.18 (s, 1H), 10.56 (s, 1H), 8.16-8.15 (m, 2H), 8.05-8.03 (d, 1H), 7.97-7.95 (d, J= 8.06 Hz 2H), 7.90-7.88 (d, J= 8.60 Hz 2H), 7.33 (s, 1H), 7.14 (s, 1H), 6.91-6.89 (d, 1H), 6.72-6.70 (d, 1H), 6.22-6.15 (m, 1H), 6.09-6.05 (d, 1H), 5.58-5.55 (d, 1H), 3.87 (d, 4H), 3.76- 3.74 (d, 5H), 3.49-3.47 (m, 1H), 2.16-2.13 (m, 1H), 1.98-1.95 (m, 1H), 1.88-1.78 (m, 2H), 151-1.41 (m, 1H), 1.22-1.13 (m, 3H). LCMS: [M+H] ⁺ : 567.42, Purity= 95.13%.

Example B0700-59

Synthesis of Compound B0700-608:

Preparation of (3R)-l-(l-acryloylpiperidin-3-yl)-N-(4-(4-morpholino-7H-pyrr olo[2,3- d]pyrimidin-6-yl)phenyl)pyrrolidine-3-carboxamide (B0700-608):

Synthesis of tert-butyl (R)-3-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl) -7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyrrolidine-l- carboxylate (289):

[00873] To an ice cold stirred solution of (3R)-l-[(tert-butoxy)carbonyl]pyrrolidine-3 -carboxylic acid 288 (1 g, 4.65 mmol) and 4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7 H- pyrrolo[2,3-d]pyrimidin-6-yl] aniline 7 (2.37 g, 5.57 mmol) in DMF (10 mL) were added ethylbi s(propan-2-yl)amine (2.40 mL, 13.9 mmol) and HATU (2.65 g, 6.97 mmol) at room temperature . Reaction mass was stirred at RT for next 16h. After completion of reaction monitored by TLC the reaction mass was poured into ice cold water (100 mL) and extracted by EtOAc (3 x 100 mL). Combined organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure. The crude was purified by column chromatography in silica gel (40g SNAP) by using eluents 70% EtOAC in Heptane to get desired product as brown solid as 289 (2.2 g, 73.75%).

'H NMR (400 MHz, DMSO-d6): 8 10.19 (s, 1H), 8.26 (s, 1H), 7.71 (s, 4H), 6.90 (s, 1H), 5.53 (s, 2H), 4.03-4.01 (m, 1H), 3.88 (s, 4H), 3.73 (s, 4H), 3.56 (m, 2H), 3.39-3.26 (m, 2H), 3.25 (d, 1H), 2.18-2.12 (m, 3H), 1.41 (s, 9H), 0.88-0.87 (m, 2H) and -0.07 (s, 9H). LCMS: [M+H] ⁺ : 623.28, Purity= 96.03%.

Synthesis of (R)-N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)- 7H-pyrrolo [2,3- d]pyrimidin-6-yl)phenyl)pyrrolidine-3-carboxamide (290):

[00874] To an ice cold solution of tert-butyl (3R)-3-({4-[4-(morpholin-4-yl)-7-{[2-

(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin -6-yl]phenyl}carbamoyl)pyrrolidine-l- carboxylate 289 (2.2 g, 3.53 mmol) in dichloromethane (20 mL) was added 4-m ethylbenzene- 1- sulfonic acid (2.43 g, 14.1 mmol) portion wise manner. The resulting reaction mixture was stirred at RT for next 16h. After completion of reaction (TLC and LCMS monitoring), reaction mixture was basify with aq NaHCOs and extracted with DCM (3x 100 mL). Combined organic layer was dried over Na2SO4, filtered and evaporated under reduced pressure to get the desired product brown solid 290 (2.1 g, Quantitative yield).

MS: [M+H] ⁺ : 523.60.

Synthesis of tert-butyl 3-((R)-3-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)meth yl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyrrolidin-l-y l)piperidine-l-carboxylate (292):

[00875] To a stirred solution of (3R)-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}pyrrolidine-3-carboxamide 290 (1 g, 1.91 mmol) and tert-butyl 3 -oxopiperidine- 1 -carboxylate 291 (572 mg, 2.87 mmol) in dichloromethane (10 mL) was added acetic acid (0.11 mL, 1.91 mmol) at 0°C. Stirred the reaction mixture at RT for next Ih under N2 atmosphere. Followed by addition of sodium cyanoborohydride (240 mg, 3.83 mmol) to the reaction mixture and stirred for 60°C for next 16h. After completion of reaction (TLC and LCMS monitoring), reaction mixture was diluted with water (100 mL) and extracted with DCM (3x 100 mL). The combined organic layer was dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure. The crude was further purified by flash column chromatography in silica gel (40 g, SNAP) using eluents 5% MeOH in DCM to get desired product as yellow solid 292 (0.7 g, 43.54%).

^X H NMR (400 MHz, DMSO-d6): 5 10.19 (s, IH), 8.25 (s, IH), 7.70 (m, 4H), 6.89 (s, IH), 5.53 (s, 2H), 3.87 (m, IH), 3.72 (s, 4H), 3.63 (s, 4H), 3.60 (m, 2H), 3.39-3.26 (m, 2H), 2.18-2.12 (m, 3H),1.98- 1.96 (m, 4H), 1.74 (m, 2H), 1.57-1.54 (m, 2H), 1.39 (m 9H), 1.36-1.34 (m IH), 0.88-0.86 (m, 2H) and -0.086 (s, 9H). LCMS: [M+H] ⁺ : 706.60, Purity= 84.55%.

Synthesis of (3R)-N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phen yl)-l-(piperidin-3- yl)pyrrolidine-3-carboxamide (293):

[00876] To an ice cold stirred solution of tert-butyl 3-[(3R)-3-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)pyrrolidin-l- yl]piperidine-l -carboxylate 292 (0.6 g, 850 pmol) in dichloromethane (10 mL) was added trifluoroacetic acid (2.5 mL) and stirred at room temperature for next 2h. The reaction mixture was concentrated under reduced pressure. The crude was basified by aq NH4OH and extracted with 10% MeOH in DCM (2x100 mL). Combined organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get desired product as yellow solid 293 (0.3 g, 60.86%). LCMS: [M+H] ⁺ : 476.34, Purity= 83.55%.

Synthesis of (3R)-l-(l-acryloylpiperidin-3-yl)-N-(4-(4-morpholino-7H-pyrr olo[2,3-d]pyrimidin- 6-yl)phenyl)pyrrolidine-3-carboxamide (B0700-608):

[00877] To an ice cold stirred solution of (3R)-N-{4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3- d]pyrimidin-6-yl]phenyl}-l-(piperidin-3-yl)pyrrolidine-3-car boxamide 293 (140 mg, 294 pmol) in DMF (3 mL) were added tri ethylamine (0.16 mL, 1.18 mmol) and prop-2-enoyl chloride 27 (29.3 mg, 324 pmol) in drop wise manner. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS monitoring), The reaction mixture was poured into ice cold water (100 mL) and extracted with 25% IP A in Chloroform (3 X 100 mL). Combined organic layer was dried over Na2SO4, filtered through cotton plug. Filtrate was concentrate under reduced pressure to get crude. Crude was purified through RP-HPLC purification to get desired product as off white solid B0700-608 (24 mg, 15.24%).

¹ HNMR (400 MHz, DMSO-d6): 5 12.13 (s, 1H), 10.00 (s, lH), 8.16 (s, 1H), 7.85-7.82 (m, 2H), 7.65- 7.63 (d, J= 8.0 Hz 2H), 7.09 (s, 1H), 6.83-6.76 (m, 1H), 6.09-6.05 (d, 1H), 5.67-5.62 (d, 1H), 4.23 (m, 1H), 3.99-3.96 (m, 1H), 3.93 (d, 4H), 3.76-3.74 (d, 4H), 3.16-2.79 (m, 5H), 2.62-2.60 (m, 2H), 2.09- 2.08 (m, 1H), 1.97-1.96 (m, 3H), 1.67-1.66 (m, 1H) and 1.46-1.36 (m, 2H). LCMS: [M+H] ⁺ : 530.44, Purity= 99.21%.

Example B0700-60

Synthesis of Compound B0700-609:

Preparation of l'-acryloyl-N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-[l,4'- bipiperidine]-4-carboxamide (B0700-609):

Synthesis of tert-butyl 4-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)piperidine-l-c arboxylate (295):

[00878] To an ice cold stirred solution of l-[(tert-butoxy)carbonyl]piperidine-4-carboxylic acid 294 (1 g, 4.36 mmol) in DMF (5 mL), were added 4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 7 (2.23 g, 5.23 mmol), DIPEA (2.25 mL, 13.1 mmol) and HATU (2.49 g, 6.54 mmol) at room temperature. The resulting reaction mixture was stirred at room temperature for next 16h. After completion of reaction (TLC monitoring), the reaction mass was poured into ice-cold water (200 mL), solid was precipitated and filtered through sintered. Solid product was dried under high vacuum get the desired product as brown solid 295 (2.4 g, 65%).

'H NMR (400 MHz, DMSO-d6): 5 10.07 (s, 1H), 8.25 (s, 1H), 7.73-7.70 (m, 4H), 6.88 (s, 1H), 5.53 (s, 2H), 4.01-4.00 (d, 1H), 3.99 (s, 4H), 3.73 (s, 4H), 3.63-3.59 (t, 2H), 2.94 (m, 1H), 2.73-2.68 (m, 1H), 1.80-1.77 (m, 1H), 1.53-1.50 (d, 2H), 1.41 (s, 9H), 1.25 (m, 3H), 0.87-0.83 (t, 2H) and -0.076 (s, 9H). LCMS: [M+H] ⁺ : 637.29, Purity= 84.19%.

Synthesis of N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-p yrrolo [2,3- d]pyrimidin-6-yl)phenyl)piperidine-4-carboxamide (296):

[00879] To an ice cold stirred solution of tert-butyl 4-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)piperidine-l- carboxylate 295 (1.2 g, 1.88 mmol) in di chloromethane (20 mL) was added 4-m ethylbenzene- 1- sulfonic acid hydrate (1.43 g, 7.54 mmol) at 0°C, stirred reaction mixture at rt for next 16h. After reaction completion (TLC monitoring), quenched with aq NaHCOs (100 mL) and extracted with DCM (2 x 100 mL). Organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. Crude was triturated with diethyl ether to get desired product as brown solid 296 (0.8 g, 79%).

'H NMR (400 MHz, DMSO-d6): 89.99 (s, 1H), 8.25 (s, 1H), 7.73-7.67 (m, 4H), 6.88 (s, 1H), 5.53 (s, 2H), 3.88 (s, 4H), 3.73 (s, 4H), 3.63-3.59 (t, 2H), 3.03-3.00 (d, 2H), 2.68 (m, 2H), 2.50 (m, 2H), 1.72- 1.70 (m, 2H), 1.55-1.53 (d, 2H), 1.25 (m, 3H), 0.87-0.83 (t, 2H) and -0.076 (s, 9H). LCMS: [M+H] ⁺ : 537.43, Purity= 84.19%.

Synthesis of tert-butyl 4-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)-[l,4'-bipiper idine]-l'-carboxylate (298): [00880] To an ice cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}piperidine-4-carboxamide 296 (0.6 g, 1.12 mmol) and tert-butyl 4-oxopiperidine-l -carboxylate 297 (445 mg, 2.24 mmol) in dichloromethane (10 mL) was added acetic acid (67.1 mg, 1.12 mmol) and resulting reaction mass was stirred for 15 min at same temperature. Followed by addition of NaCNBHs (211 mg, 3.35 mmol) in portion wise manner, the reaction was allowed to stirred at 65°C for 16h. After completion of reaction (TLC monitoring), solvent was evaporated up to dryness to get crude residue. Crude was purified by flash column chromatography in silica gel using (10% MeOH :DCM) to get viscous gummy solid 298 (580 mg, 43%).

LCMS: [M+H] ⁺ : 720.66, Purity= 80.19%.

Synthesis of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-[ l,4'-bipiperidine]- 4-carboxamide (299):

[00881] To an ice cold tert-butyl 4-({4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methy l}- 7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}carbamoyl)-[l,4'-bipi peridine]-r-carboxylate 298 (580 mg, 806 pmol) in dichloromethane (5 mL, 78.1 mmol) was added trifluoroacetic acid (2.5 mL) at 0°C, stirred reaction mixture for next 2h at room temperature. After LCMS monitoring the reaction mixture was concentrated under reduced pressure to get crude. Crude was then diluted with water (50 mL) and extracted with 10% IP A in CHCL (2 x 100 mL). The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get the desired product as brown solid 299 (220 mg, 56%).

MS: [M+H] ⁺ : 490.25.

Synthesis of l'-acryloyl-N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-[l,4'- bipiperidine]-4-carboxamide (B0700-609):

[00882] To an ice cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-[l,4'-bipiperidine]-4-carboxamide 299 (220 mg, 449 pmol) in dimethylformamide (7.33 mL, 94.7 mmol) were added triethylamine (189 pL, 3 eq., 1.35 mmol) and prop-2-enoyl chloride 27 (40.7 mg, 449 pmol) drop wise manner. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC monitoring), the resulting reaction mass was evaporated to dryness and the crude residue was purified through RP-HPLC purification using eluent as 5mM Ammonium Bicarbonate in water/ Acetonitrile and column using Waters Xselect Phenyl- Hexyl(19*250mm,5pm to get desired product as white solid B0700-609 ( 30 mg, 12.28%).

'H NMR (400 MHz, DMSO-d6): 5 12.13 (s, 1H), 9.91 (s, lH), 8.16 (s, 1H), 7.83-7.81 (d,J= 8.0, 2H), 7.66-7.64 (d, J= 8.0, 2H), 7.08 (s, 1H), 6.83-6.76 (m, 1H), 6.09-6.04 (d, 1H), 5.66-5.63 (d, 1H), 4.45- 4.43 (m, 1H), 4.08-4.03 (m, 1H), 3.88-3.86 (d, 4H), 3.75-3.74 (d, 4H), 3.04-2.98 (m, 1H), 2.92-2.89 (d, 2H), 2.62-2.60 (m, 2H), 2.29-2.26 (m, 1H), 2.19-2.14 (m, 2H), 1.78-1.75 (m, 4H), 1.65-1.60 (m, 2H) and 1.34-1.31 (m, 2H). LCMS: [M+H] ⁺ : 544.42, Purity= 98.52%.

Example B0700-61

Synthesis of Compound B0700-610:

Preparation of l'-acryloyl-N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-[l,4'- bipiperidine]-4-sulfonamide (B0700-610): Synthesis of tert-butyl 4-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7 H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)piperidine-l-c arboxylate (301):

[00883] To stirred solution of tert-butyl 4-(chlorosulfonyl)piperidine-l -carboxylate 300 (2 g, 7.05 mmol) in dichloromethane (20 mL) were added 4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin- 6-yl]aniline 7 (2.29 g, 7.75 mmol) and triethylamine (1.17 mL, 8.46 mmol). The resulting reaction mixture was stirred at room temp for next 16h. After completion of reaction (TLC and LCMS monitoring), reaction mixture was poured in to ice cold water (100 mL) and extracted in dichloromethane (4 x 500 mL). Combined organic layer was dried over Na2SO4, filtered and evaporated under reduced pressure to get the desired compound as off-white solid. 301 (1.1 g, 1.48 mmol).

MS: [M+H] ⁺ : 673.49.

Synthesis of N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-p yrrolo [2,3- d]pyrimidin-6-yl)phenyl)piperidine-4-sulfonamide (302):

[00884] To an ice cold solution of tert-butyl 4-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}sulfamoyl)piperidine-l- carboxylate 301 (1.1 g, 1.63 mmol) in dichloromethane (15 mL) was added 4-m ethylbenzene- 1- sulfonic acid (1.13 g, 6.54 mmol) in portion wise. The resulting reaction mixture was stirred at RT for next 16h. After completion of reaction (TLC and LCMS monitoring), reaction mixture was basify with aq NaHCOs and extracted with DCM (3x 100 mL). Combined organic layer was dried over Na2SO4, filtered and evaporated under reduced pressure to get the desired product brown solid 302 (1.20 g, 87%).

MS: [M+H] ⁺ : 573.24.

Synthesis of tert-butyl 4-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7 H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)-[l,4'-bipiper idine]-l'-carboxylate (303):

[00885] To a stirred solution of N-{4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl }-7H- pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}piperidine-4-sulfonamide 302 (1 g, 1.75 mmol) and tert-butyl 4-oxopiperidine-l -carboxylate 297 (522 mg, 2.62 mmol) in di chloromethane (10 mL) was added DIPEA (0.90 mL, 5.24 mmol) at 0°C. The reaction mixture was stirred at RT for next 2h under N2 atmosphere. Followed by addition of sodium tri acetoxyb orohydri de (740 mg, 3.49 mmol) at room temperature and stirred the reaction mixture RT for next 16h. After completion of reaction (TLC and LCMS monitoring), the reaction mixture was diluted with water (100 mL) and extracted with DCM (2 x 100 mL). The combined organic layer was dried over anhydrous ISfeSCU, filtered and concentrated under reduced pressure to get crude. Which was purified by flash column chromatography in silica gel (230-400M) using eluent 70% ethyl acetate in heptane to get desired product as yellow solid 303 (470 mg, 35% mmol).

'H NMR (400 MHz, DMSO-d6): 5 8.25 (s, 1H), 7.69-7.67 (d, J= 8.0, 2H), 7.33-7.31 (d, J= 8.0, 2H), 6.88 (s, 1H), 5.54 (s, 2H), 3.79-3.78 (m, 6H), 3.72 (s, 4H), 3.58-3.56 (t, 2H), 3.00-2.99 (m, 2H), 2.88 (d, 2H), 2.18-2.16 (m, 2H), 1.95-1.93 (m, 2H), 1.63-1.61 (m, 2H), 1.36 (s, 9H), 1.22-1.21 (m, 4H), 1.13-1.12 (m, 2H), 0.84-0.82 (m, 2H) and -0.10 (s, 9H). LCMS: [M+H] ⁺ : 756.49, Purity= 84%.

Synthesis of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-[ l,4'-bipiperidine]- 4-sulfonamide (304):

[00886] To an ice cold solution of tert-butyl 4-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl } -7H-pyrrolo[2,3 -d]pyrimidin-6-yl]phenyl } sulfamoyl)-[ 1 ,4'- bipiperidine]-l'-carboxylate 303 (470 mg, 622 pmol) in dichloromethane (5 mL) was added 0°C in trifluoroacetic acid (2.5 mL) in drop wise manner. The resulting reaction mixture was stirred at room temp for next 3h. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated under reduced pressure. Crude was basify with aq NH4OH and extraction with 20% IPA: CHCL (3 x 50 mL). The combined organic layers was washed with brine (100 mL), dried over anhydrous ISfeSCL, filtered and concentrated under reduced pressure to get desired product as brown solid 304 (0.3 mg, 68.85%).

MS: [M+H] ⁺ : 526.42.

Synthesis of l'-acryloyl-N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6- yl)phenyl)-[l,4'- bipiperidine]-4-sulfonamide (B0700-610):

[00887] To an ice cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl]-[l,4'-bipiperidine]-4-sulfonamide 304 (0.3 g, 571 pmol) in dimethylformamide (4 mL) were added triethylamine (241 pL, 1.71 mmol) and prop-2-enoyl chloride 27 (62 mg, 685 pmol) in drop wise manner. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS monitoring), the reaction mixture was diluted water (100 mL) and extracted with 25% IPA/Chloroform (3 X 100 mL). Combined organic layer was dried over Na2SO4, filtered through cotton plug. Filtrate was concentrate under reduced pressure. Crude residue was purified through RP-HPLC purification to get desired product as yellow solid B0700-610 (25 mg, 7.41%) .

'H NMR (400 MHz, DMSO-d6): 5 12.17 (s, 1H), 9.92 (s, lH), 8.17 (s, 1H), 7.84-7.82 (d,J= 8.0, 2H), 7.27-7.25 (d, J= 8.0, 2H), 7.08 (s, 1H), 6.79-6.73 (m, 1H), 6.07-6.02 (d, 1H), 5.65-5.61 (d, 1H), 4.41- 4.38 (m, 1H), 4.04-4.01 (m, 1H), 3.88-3.86 (d, 4H), 3.75-3.74 (d, 4H), 3.02-2.87 (m, 4H), 2.50 (d, 2H), 2.14-2.09 (d, 2H), 1.95-1.92 (d, 2H), 1.70-1.56 (m, 4H) and 1.27-1.20 (m, 2H). LCMS: [M+H] ⁺ : 580.20, Purity= 98.48%.

Example B0700-62

Synthesis of Compound B0700-611:

Preparation of l-(l-acryloylpiperidin-3-yl)-N-(4-(4-morpholino-7H-pyrrolo[2 ,3-d]pyrimidin-6- yl)phenyl)pyrrolidine-3-sulfonamide (B0700-611):

Synthesis of tert-butyl 3-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7 H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)pyrrolidine-l- carboxylate (306):

[00888] To a stirred solution of 4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7 H- pyrrolo[2,3-d]pyrimidin-6-yl] aniline 7 (550 mg, 1.29 mmol) in di chloromethane (8 mL) was added DIPEA (1.1 mL, 6.46 mmol) at RT under N2 atmosphere and stirred for next 5 min. Then 4- (dimethylamino)pyridin-l-ium (15.9 mg, 129 pmol) was added into reaction mixture and stirred for 10 min. Then stock solution of tert-butyl (3R)-3-(chlorosulfonyl)pyrrolidine-l -carboxylate 305 (1.05 g, 3.88 mmol) in dichloromethane (12 mL) was added at 0°C to the reaction mixture. The reaction mixture was stirred at RT for 16h. After completion of reaction (TLC monitoring), the reaction mixture was diluted with water (50 mL) and extracted with DCM (2 x 50 mL). The organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified by flash column chromatography (silica gel, 12 g SNAP) using eluents 40% ethyl acetate in heptane to get the desired product as brown solid 306 (580 mg, 46%).

MS: [M+H] ⁺ : 659.33.

Synthesis of N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-p yrrolo [2,3- d]pyrimidin-6-yl)phenyl)pyrrolidine-3-sulfonamide (307):

[00889] To an ice-cold stirred solution of tert-butyl 3-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl } -7H-pyrrolo[2,3 -d]pyrimidin-6-yl]phenyl } sulfamoyl)pyrrolidine- 1 - carboxylate 306 (230 mg, 349 pmol) in dichloromethane (10 mL) was added 4-m ethylbenzene- 1- sulfonic acid (240 mg, 1.4 mmol) under N2 atmosphere. The reaction mixture was stirred at RT for 2h. After completion of reaction (TLC monitoring), the reaction mixture was quenched with aq. NaHCOs and extracted with DCM (3 x 50 mL). The combined organic layer was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure to get the desired product as white solid 307 (160 mg, 74%).

'H NMR (400 MHz, DMSO-d6): 8 8.25 (s, 1H), 7.71-7.69 (d, J= 8.0 Hz, 2H), 7.32-7.30 (d, J= 8.06 Hz, 2H), 6.88 (s, 1H), 5.54 (s, 2H), 3.80 (s, 4H), 3.72 (s, 4H), 3.69-3.66 (m, 3H), 2.98 (d, 2H), 2.14- 2.09 (d, 2H), 2.15 (m, 2H), 2.11-1.98 (m, 2H), 1.22 (m, 1H), 0.85-0.83 (m, 2H) and -0.93 (s, 9H). LCMS: [M+H] ⁺ : 559.22, Purity= 90%.

Synthesis of tert-butyl 3-(3-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl )-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)pyrrolidin-l-y l)piperidine-l-carboxylate (308):

[00890] To a stirred solution of N-{4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl }-7H- pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}pyrrolidine-3-sulfonamid e 307 (180 mg, 322 pmol) in dichloromethane (5 mL) was added tert-butyl 3 -oxopiperidine- 1 -carboxylate 291 (96.3 mg, 483 pmol) at RT under N2 atmosphere. Followed by addition of acetic acid (19.3 mg, 322 pmol) to the reaction mixture at 0°C and stirred for 30 min at RT. Then boron(3+) sodium iminomethanide trihydride (40.5 mg, 644 pmol) was added to the reaction mixture. The reaction mixture was stirred at 60°C for next 5h. After completion of reaction (TLC monitoring), the reaction mixture was quenched with aq. NaHCCL and extracted with DCM (3x 50 mL). The combined organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get the desired product as brown solid 308 (110 mg, 20%).

MS: [M+H] ⁺ : 742.30.

Synthesis of N-(4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)-l -(piperidin-3- yl)pyrrolidine-3-sulfonamide (309):

[00891] To an ice-cold stirred solution of tert-butyl 3-[3-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl } -7H-pyrrolo[2,3 -d]pyrimidin-6-yl]phenyl } sulfamoyl)pyrrolidin- 1 - yl]piperidine-l -carboxylate 308 (0.1 g, 135 pmol) in dichloromethane (2 mL) was added trifluoroacetic acid (0.5 mL) under N2 atmosphere. The reaction mixture was stirred at RT for 1.5h. After LCMS monitoring the reaction mixture was concentrated under reduced pressure. Crude was then dissolved in 1,4-dioxane (2 mL) was added and ethane- 1,2-diamine (24.3 mg, 404 pmol) at 0°C. The reaction mixture was stirred at 70°C for 30 min. After completion of reaction (LCMS monitoring), the reaction mixture was diluted with water (20 mL) and extracted with 10% IP A in CHCL (2 x 20 mL). The organic layer was washed with brine (50 mL), dried over ISfeSCL, filtered and concentrated under reduced pressure to get the desired product as brown solid 309 (60 mg, 78% )•

MS: [M+H] ⁺ : 512.34.

Synthesis of l-(l-acryloylpiperidin-3-yl)-N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)pyrrolidine-3-sulfonamide (B0700-611):

[00892] To an ice-cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-l-(piperidin-3-yl)pyrrolidine-3-sulfonamide 309 (90 mg, 176 pmol) in N,N- dimethylformamide (0.70 mL) was added triethylamine (81 pL, 880 pmol) and stirred for 10 min. Then prop-2-enoyl chloride 27 (12.8 mg, 211 pmol) in N,N-dimethylformamide (0.30 mL) was added to the reaction mixture at same temperature. The reaction mixture was stirred for 15min. After completion of reaction (LCMS monitoring), the reaction mixture was poured into ice-cooled water (50 mL) and extracted with 10% IP A in CHCL (2 x 50 mL). The combined organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified through RP-HPLC purification using eluent as 5mM ammonium bicarbonate in water/acetonitrile and column using Waters Xselect Phenyl-Hexyl(19*250mm, 5pm to get desired product as pale yellow solid B0700-611 (1.4 mg, 2%). 'H NMR (400 MHz, DMSO-d6): 8 12.17 (s, 1H), 9.89 (s, 1H), 8.17 (s, 1H), 7.86-7.84 (d, J= 8.0, 2H), 7.27-7.25 (d, J= 8.0, 2H), 7.09 (s, 1H), 6.79-6.72 (m, 1H), 6.07-6.01 (d, 1H), 5.64-5.61 (d, 1H), 4.24-4.19 (m, 1H), 4.00 (m, 1H), 3.87-3.86 (d, 4H), 3.75-3.74 (d, 4H), 3.08-2.78 (m, 6H), 2.08-2.03 (d, 4H), 1.88-1.86 (m, 1H), 1.65 (m, 1H) and 1.38-1.31 (m, 2H). LCMS: [M+H] ⁺ : 566.38, Purity= 97.56%.

Example B0700-63

Synthesis of Compound B0700-612:

Preparation of 4-(l-acryloylpiperidin-4-yl)-N-(4-(4-morpholino-7H-pyrrolo[2 ,3-d]pyrimidin-6- yl)phenyl)piperazine-l-sulfonamide (B0700-612):

Synthesis of l-((lH-imidazol-l-yl)sulfonyl)-3-methyl-lH-imidazol-3-ium. trifluoromethanesulfonate (311):

[00893] To an ice cold stirred solution of l-(lH-imidazole-l-sulfonyl)-lH-imidazole 310 (5 g, 25.2 mmol) in dichloromethane (50 mL) was added methyl trifluoromethanesulfonate (3.73 g, 22.7 mmol) drop wise in 15 min. The reaction mass was stirred at same temperature for 2h. After completion of reaction (TLC monitoring), the solid precipitates was filtered and dried under high vacuum to get desired product as white solid 311 (7.7 g, 84%).

^X H NMR (400 MHz, D2O): 8 8.53 (s, 1H), 9.89 (s, 1H), 8.19 (s, 1H), 7.81 (s, 1H), 7.74 (s, 1H), 7.50- 7.45 (d, 1H), 7.29 (s, 1H) and 4.03 (s, 3H). Synthesis of tert-butyl 4-((lH-imidazol-l-yl)sulfonyl)piperazine-l-carboxylate (312):

[00894] To a stirred solution of tert-butyl piperazine- 1 -carboxylate 43 (3 g, 16.1 mmol) in acetonitrile (30 mL) was added l-(lH-imidazole-l-sulfonyl)-3-methyl-lH-imidazol-3-ium trifluoromethanesulfonate 311 (5.84 g, 16.1 mmol), stirred at room temperature for next 16h. After completion of SM (TLC monitoring), reaction mixture was concentrated under reduced pressure. Crude was dissolved in EtOAc (2x 100 mL) and washed with brine solution (50 mL). Organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. Which was purified by flash chromatography in silica gel (230-400M, 40g SNAP) using eluent 50% EtOAc in heptane to get desired product as white solid 312 (1.70 g, 33.36%).

'H NMR (400 MHz, CDC13): 8 8.07 (s, 1H), 7.26 (s, 1H), 7.21 (s, 1H), 3.55-3.52 (t, 4H), 3.18-3.13 (t, 4H), and 1.43 (s, 9H). LCMS: [M+H] ⁺ :317.3, Purity=88%.

Synthesis of l-((4-(tert-butoxycarbonyl)piperazin-l-yl)sulfonyl)-3-methyl -lH-imidazol-3-ium trifluoromethanesulfonate (313):

[00895] To an ice stirred solution of tert-butyl 4-(lH-imidazole-l-sulfonyl)piperazine-l-carboxylate 312 (1.7 g, 5.37 mmol) in dichloromethane (20 mL) was added methyl trifluoromethanesulfonate (970 mg, 5.91 mmol) at 0°C, stirred reaction mixture at room temperature for next 2h. After completion of SM (TLC monitoring) diluted with n-hexane and solid was filtered out. Solid was dried under high vacuum to get white solid product 313 (2.3 g, 89%).

'H NMR (400 MHz, DMSO-d6): 8 9.79 (s, 1H), 8.15 (s, 1H), 7.97 (s, 1H), 3.88 (s, 3H), 3.47 (t, 4H), 3.34 (t, 4H) and 1.38 (s, 9H). LCMS: [M+H] ⁺ :331.26.3, Purity=97%.

Synthesis of tert-butyl 4-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7 H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)piperazine-l-c arboxylate (314):

[00896] To a stirred solution of l-({4-[(tert-butoxy)carbonyl]piperazin-l-yl}sulfonyl)-3-meth yl-lH- imidazol-3-ium trifluoromethanesulfonate 313 (2.6 g, 5.41 mmol) and 4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 7 (2.3 g, 5.41 mmol) in acetonitrile (23.6 mL), reaction mixture was heated at 80°C for next 16h. After completion of SM (TLC monitoring), acetonitrile was evaporated under reduced pressure. Crude was dissolved in Ethyl acetate (100 mL) and washed with water (50 mL) and brine solution (50 mL). Organic layer was dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. Crude was purified by column chromatography in silica gel 230-400M (12g SNAP) using eluents 25-35% EtOAc:heptane to get desired product as pale yellow solid 314 (2.38 g, 65.26%).

^X H NMR (400 MHz, DMSO-d6): 5 10.20 (s, 1H), 8.21 (s, 1H), 7.69-7.67 (d, J= 8.0, 2H), 7.30-7.28 (d, J= 8.0, 2H), 6.88 (s, 1H), 5.53 (s, 2H), 4.02-4.01 (d, 4H), 3.72 (s, 4H), 3.58-3.54 (t, 2H), 3.29 (d, 4H), 3.09-308 (d, 4H), 1.35 (s, 9H), 0.84-0.80 (m, 2H) and -0.077 (s, 9H). LCMS: [M+H] ⁺ : 674.22, Purity= 86%.

Synthesis of N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-p yrrolo [2,3- d]pyrimidin-6-yl)phenyl)piperazine-l-sulfonamide (315):

[00897] To a stirred solution of tert-butyl 4-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl } -7H-pyrrolo[2,3 -d]pyrimidin-6-yl]phenyl } sulfamoyl)piperazine- 1 - carboxylate 314 (2.3 g, 3.41 mmol) in dichloromethane (53.1 mL) was added 4-m ethylbenzene- 1- sulfonic acid (2.35 g, 13.7 mmol), stirred at room temperature for next 16h. After completion of SM (TLC monitoring), reaction mixture was quenched with aq NaHCOs (50 mL) and extracted with DCM (2 x 100 mL). Organic layer dried over Na2SO4, filtered and concentrated under reduced pressure to get desired product as light brown viscous liquid 315 (1.60 g, 81.7%).

MS: [M+H] ⁺ : 574.43.

Synthesis of tert-butyl 4-(4-(N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl )-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)sulfamoyl)piperazin-l-yl )piperidine-l-carboxylate (316): [00898] To a stirred solution of N-{4-[4-(morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl }-7H- pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}piperazine-l-sulfonamide 315 (1.2 g, 2.09 mmol) and tertbutyl 4-oxopiperidine-l -carboxylate 297 (1.25 g, 6.27 mmol) in methanol (16 mL) and dimethylformamide (4 mL) was added acetic acid (0.2 mL, 209 pmol) and stirred reaction mixture at room temperature for next Ih. After addition of sodium cyanob orohydri de (394 mg, 6.27 mmol), reaction mixture was stirred at 60°C for next 4h. After reaction monitoring (TLC), quenched with ice cold water (250 mL) and extracted with ethyl acetate (2 x 250 mL). The combined organic layer was washed with brine solution (2 x 50 mL) dried over Na2SO4, filtered and concentrated under reduced pressure. The crude was purified over combi flash in silica gel (40g ,SNAP) using eluents 7.5% MeOH:DCM to get desired product as yellow viscous liquid 316 (870 mg, 42.65%).

'H NMR (400 MHz, DMSO-d6): 8 10.15 (s, IH), 8.25 (s, IH), 8.17 (s, IH), 7.69-7.67 (d, J= 8.0, 2H), 7.29-7.27 (d, J= 8.0, 2H), 6.88 (s, IH), 5.53 (s, 2H), 3.87 (s, 5H), 3.72 (d, 4H), 3.56-3.53 (t, 2H), 3.10 (s, 4H), 2.50-2.40 (d, 7H), 1.62-1.60 (d, 2H),1.35 (m, 10H), 1.18-1.16 (m, 3H), 0.85-0.83 (d, 2H), and -0.09 (s, 9H). LCMS: [M+H] ⁺ : 757.57, Purity= 88%.

Synthesis of N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)-4-(piperidin-4- yl)piperazine-l-sulfonamide (317):

[00899] To an ice cold stirred solution of tert-butyl 4-[4-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl } -7H-pyrrolo[2,3 -d]pyrimidin-6-yl]phenyl } sulfamoyl)piperazin- 1 - yl]piperidine-l -carboxylate 316 (850 mg, 1.12 mmol) in dichloromethane (20 mL) was added trifluoroacetic acid (10 mL) in 0°C, stirred reaction mixture at room temperature for next 2h. After completion of SM (TLC monitoring), reaction mixture was directly concentrated under reduced pressure to get desired product as light brown viscous liquid 317 (800 mg, as TFA salt quantification not done).

LCMS: [M+H] ⁺ : 527.27, Purity= 81%.

Synthesis of 4-(l-acryloylpiperidin-4-yl)-N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)piperazine-l-sulfonamide (B0700-612):

[00900] To an ice cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-4-(piperidin-4-yl)piperazine-l -sulfonamide 317 (550 mg, 1.04 mmol) in dimethylformamide (11 mL) were added tri ethylamine (881 pL, 6.27 mmol) and prop-2-enoyl chloride 27 (94.5 mg, 1.04 mmol) at 0°C, stirred for next 10 min at same temperature. After completion of SM (TLC monitoring), quenched with ice cold water (50 mL) and desired product was extracted with 20% IPA CHCL (3 x 100 mL). Crude was submitted for RP-HPLC purification to get desired product as white solid B0700-612 (84 mg, 13.85%).

^X H NMR (400 MHz, DMSO-d6): 6 12.16 (s, 1H), 10.02 (s, 1H), 8.17 (s, 1H), 7.84-7.82 (d, J= 8.0, 2H), 7.24-7.22 (d, J= 8.0, 2H), 7.08 (s, 1H), 6.76-6.69 (m, 1H), 6.05-6.01 (dd, 1H), 5.62-5.59 (dd, 1H), 4.38-4.35 (d, 1H), 4.01-4.39 (m, 1H), 3.88-3.87 (d, 4H), 3.75-3.74 (d, 4H), 3.08-2.78 (m, 6H), 2.98-2.92 (d, 1H), 2.43 (s, 4H), 1.69-1.66 (d, 2H) and 1.19 (m, 2H). LCMS: [M+H] ⁺ : 581.48, Purity= 98.86%. Example B0700-64

Synthesis of Compound B0700-613:

Preparation of (R)-N-(l-((2-(((4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)cyclohexyl)amino)methyl)pyridin-4-yl)methyl)piperidin-3-y l)acrylamide (B0700-613):

Synthesis of tert-butyl 4-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H- pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)carbamoyl)piperazine- 1-carboxylate (318):

[00901] To an ice cold stirred solution of 4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 7 (1 g, 2.35 mmol) in Isopropyl acetate (20 mL) was added phenyl carb onochlori date (441 mg, 2.82 mmol), ISfeCCL (299 mg, 2.82 mmol) and water (2 mL) under nitrogen atmosphere. The reaction mixture was stirred at room temperature for next 2h. Then triethylamine (0.33 mL, 2.35 mmol) and tert-butyl piperazine- 1-carboxylate 43 (438 mg, 2.35 mmol) was added to reaction mixture and stirred at 60°C for next 16h. After completion of reaction (TLC and LCMS) monitoring, the reaction mixture was diluted water (50 mL) and extracted with EtOAc (3 X 50 mL). The combined organic layer was dried over Na2SC>4, filtered through cotton plug. Filtrate was concentrate under reduced pressure. Crude was purified by column chromatography desired eluted with 20% EtOAc/Heptane to get desired product white solid 318 (1 g, 64%).

^X H NMR (400 MHz, DMSO-d6): 3 8.75 (s, 1H), 8.25 (s, 1H), 7.65-7.58 (m, 4H), 6.87 (s, 1H), 5.53 (s, 2H), 3.88 (s, 4H), 3.73 (s, 4H), 3.65-3.61 (t, 2H), 3.44 (d, 4H), 3.37 (s, 4H), 1.42 (s, 9H), 0.88- 0.84 (m, 2H) and -0.07 (s, 9H). LCMS: [M+H] ⁺ : 638.34, Purity= 96.56%. Synthesis of N-(4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)-7H-p yrrolo [2,3- d]pyrimidin-6-yl)phenyl)piperazine-l-carboxamide (319):

[00902] To an ice cold stirred solution of tert-butyl 4-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl } -7H-pyrrolo[2,3 -d]pyrimidin-6-yl]phenyl } carbamoyl)piperazine- 1 - carboxylate 318 (1 g, 1.57 mmol) in dichloromethane (30 mL) was added 4-m ethylbenzene- 1- sulfonic acid (1.08 g, 6.27 mmol) portion wise. The reaction mixture was stirred at room temperature for next 16h. After completion of reaction (LCMS and TLC) monitoring, the reaction mixture was basify with aq NaHCO3 (100 mL) and extracted with DCM (2x 200 mL). Combined organic layer was washed with brine solution (100 mL), dried over ISfeSCU, filtered and concentrated under reduced pressured to get crude. Crude was further triturated with diethyl ether to get desired product as off white solid 319 (0.7 g, 82%).

LCMS: [M+H] ⁺ : 538.21, Purity= 99.48%.

Synthesis of tert-butyl 4-(4-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl)- 7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)piperazin-l-yl )piperidine-l-carboxylate (320): [00903] To an ice cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}piperazine-l-carboxamide 319 (670 mg, 1.25 mmol) in dichloromethane (25 mL) was added DIPEA (0.42 mL, 2.49 mmol) and tert-butyl 4-oxopiperidine-l -carboxylate 297 (372 mg, 1.87 mmol) under nitrogen atmosphere. The reaction mixture was stirred at room temperature for Ih. Then sodium bis(acetyloxy)boranuidyl acetate (792 mg, 3.74 mmol) was added the reaction mixture stirred at 60°C for next 16h. After completion of reaction (TLC and LCMS) monitoring, the reaction mixture was quenched sat. NaHCOs (50 mL) and extracted with DCM (3 x 100 mL). The combined organic layer was dried over Na2SO4, filtered and concentrate under reduced pressure. Crude was purified by column chromatography in silica (230-400M) using eluents 5% MeOH in DCM to get desired product was gummy solid 320 (0.7 g, 66.23%).

LCMS: [M+H] ⁺ : 721.6, Purity= 85%.

Synthesis of N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)-4-(piperidin-4- yl)piperazine-l-carboxamide (321):

[00904] To an ice cold stirred solution of tert-butyl 4-[4-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)piperazin-l- yl]piperidine-l -carboxylate 320 (0.6 g, 832 pmol) in dichloromethane (18 mL) was added trifluoroacetic acid (6 mL), stirred at room temperature for next 2h. After completion of SM (TLC and LCMS monitoring), reaction mixture concentrated under reduced pressure. Crude residue was dissolved in 1,4-dioxane (15 mL) followed by addition of ethane- 1,2-diamine (1 mL) further stirred at 80°C for next 2h. After completion of reaction (LCMS monitoring), the reaction mixture was poured into water (50 mL) and extracted with 20% IPA in Chloroform (3 x 50 mL). Combined organic layer was dried over Na2SO4, filtered and concentrate under reduced pressure to get desired product as light brown solid 321 (495 mg, quantitative).

LCMS: [M+H] ⁺ : 491.26, Purity= 86.46%.

Synthesis of 4-(l-acryloylpiperidin-4-yl)-N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)piperazine-l-carboxamide (B0700-613):

[00905] To an ice cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-4-(piperidin-4-yl)piperazine-l -carboxamide 321 (495 mg, 1.01 mmol) in DMF (7.92 mL) was added triethylamine (567 pL, 4.04 mmol) and prop-2-enoyl chloride 27 (110 mg, 1.21 mmol) in drop wise manner. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS) monitoring, the resulting reaction mass was poured in ice cold water (40 mL) and extracted with 20% IPA in Chloroform (3 X 50 mL). Combined organic layer was dried over Na2 SCU. filtered through cotton plug. Filtrate was concentrate under reduced pressure to get crude residue. Crude residue was purified through RP-HPLC purification using eluent as 5mM Ammonium Bicarbonate in water/ Acetonitrile and column using Waters Xselect Phenyl- Hexyl (19*250mm,5pm to get desired product as white solid B0700-613 (41 mg, 7.31%).

'H NMR (400 MHz, DMSO-d6): 6 12.08 (s, 1H), 8.59 (s, 1H), 8.15 (s, 1H), 7.78-7.76 (d, J= 8.0, 2H), 7.53-7.51 (d, J= 8.0, 2H), 7.04 (s, 1H), 6.83-6.76 (m, 1H), 6.09-6.05 (dd, 1H), 5.66-5.63 (dd, 1H), 4.45-4.42 (d, 1H), 4.09-4.06 (d, 1H), 3.88-3.87 (d, 4H), 3.75-3.74 (d, 4H), 3.43 (s, 4H), 3.06- 3.00 (t, 1H), 2.60 (s, 6H), 1.81-1.79 (d, 2H) and 1.33-1.28 (m, 2H). LCMS: [M+H] ⁺ : 545.40, Purity= 97.34%. Example B0700-65

Synthesis of Compound B0700-614:

Compound was prepared or can be prepared following the synthetic scheme depicted below and using the general methods and procedures described herein.

Preparation of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-morpholin o-lH- pyrrolo[3,2-c]pyridin-2-yl)phenyl)picolinamide (B0700-614):

Synthesis of 4-chloro-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrrolo[3,2 -c]pyridine (323): [00906] To an ice cold stirred solution of 4-chloro-lH-pyrrolo[3,2-c]pyridine 322 (2 g, 13.1 mmol) in dimethylformamide (10 mL) was added sodium hydride (472 mg, 19.7 mmol) portion wise under N2 atmosphere. The resulting reaction mixture was stirred for 30 min. Then [2- (chloromethoxy)ethyl]trimethylsilane (2.62 g, 1.2 eq., 15.7 mmol) in DMF (2 mL) was added in drop wise manner and the reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice-cold water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The combined organic layer was washed with brine solution (3 x 100 mL), dried over Na2SO4, filtered and concentrated under reduced pressure to get crude residue which was purified over silica gel (100-200M) column chromatography using eluent 41 % EtOAc: heptane to get the desired product as yellow liquid 323 (2.3 g, 62.42%).

^X H NMR (400 MHz, DMSO-d6): 8 8.05-8.04 (d, J= 5.6 Hz, 1H), 7.70-7.69 (d, J= 3.2 Hz, 1H), 7.65- 7.64 (d, J= 4.0 Hz, 1H),), 6.623-6.616 (d, J= 2.80 Hz, 1H), 5.61 (s, 2H), 3.47-3.43 (t, 2H), 0.82-0.78 (t, 2H) and -0.10 (s, 9H). LCMS: [M+H] ⁺ : 283.09, Purity= 98.74%. Synthesis of 4-chloro-2-iodo-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrr olo[3,2-c]pyridine (324):

[00907] To an ice cold stirred solution of 4-chloro-l-{[2-(trimethylsilyl)ethoxy]methyl}-lH- pyrrolo[3,2-c]pyridine 323 (1.3 g, 4.6 mmol) in tetrahydrofuran (10 mL) was added LDA (6.89 mL 6.89 mmol) was added dropwise in the reaction mass and reaction was stirred at same temperature for Ih. Then iodine (875 mg, 6.89 mmol) was added and reaction was stirred at rt for 2h . After completion of reaction (TLC monitoring), the reaction mixture was quenched with aqueous NH4CI solution (50 mL) at 0°C followed by extraction with ethyl acetate (3 x 50 mL).The combined organic layer was washed with brine solution (2 x 50 mL), dried over ISfeSCU, filtered and evaporated under reduced pressure to get the crude residue which was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 5% ethyl acetate in heptane to get the desired product as off-white solid 324 (0.8 g, 34.9%).

'H NMR (400 MHz, DMSO-d6): 8 8.02-8.00 (d, J= 8.4 Hz, IH), 7.70-7.68 (d, J= 8 Hz, IH), 6.97 (s, IH), 5.59 (s, 2H), 3.52-3.50 (t, 2H), 0.83-0.79 (t, 2H) and -0.10 (s, 9H). LCMS: [M+H] ⁺ : 409.02, Purity= 81.26%.

Synthesis of 4-(2-iodo-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrrolo[3, 2-c]pyridin-4- yl)morpholinepyridine (325):

[00908] To a stirred solution of 4-chloro-2-iodo-l-{[2-(trimethylsilyl)ethoxy]methyl}-lH- pyrrolo[3,2-c]pyridine 324 (0.8 g, 1.96 mmol) in dimethyl sulfoxide (10 mL) and ethylbis(propan-2- yl)amine (1.26 g, 9.79 mmol) was added and morpholine 4 (853 mg, 9.79 mmol) was added in the reaction mass and reaction was heated at 140°C for 12h. After completion of reaction (TLC monitoring), the reaction mixture was cooled to room temperature quenched with ice cold-water (100 mL) and extracted with ethyl acetate (2 x 100 mL). The combined organic layer was washed with brine solution (3 x 50 mL), dried over Na2SO4, filtered and evaporated under reduced pressure to get crude residue which was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 10% ethyl acetate in heptane to get the desired product as yellow solid 325 (0.70 g, 75.51%).

'H NMR (400 MHz, DMSO-d6): 8 7.77-7.75 (d, J= 5.6 Hz, IH), 7.09-7.08 (d, J= 6 Hz, IH), 7.02 (s, IH), 5.48 (s, 2H), 3.73-3.72 (s, 4H), 3.51-3.49 (m, 6H), 0.83-0.79 (t, 2H) and -0.080 (s, 9H). LCMS: [M+H] ⁺ : 460.24, Purity= 97.36%. Synthesis of 4-(4-morpholino-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrr olo[3,2-c]pyridin-2- yl)aniline (326):

[00909] To a stirred solution of 4-(2-iodo-l-{[2-(trimethylsilyl)ethoxy]methyl}-lH-pyrrolo[3, 2- c]pyridin-4-yl)morpholine 325 (0.7 g, 1.52 mmol) in 1,4-dioxane (5.83 mL, 68.4 mmol) and water (1.46 mL, 80.9 mmol) were added (4-aminophenyl)boronic acid 6 (313 mg, 2.29 mmol) and ISfeCCL (484 mg, 4.57 mmol) at rt, The resulting reaction mixture was degassed with using nitrogen gas for 15 min. After 15 min PdC12(dppf).DCM (124 mg, 152 pmol) was added and reaction mixture was heated 100°C for 16h. After the completion of reaction (TLC monitoring), the reaction mixture was filtered through celite bed followed by washing with ethyl acetate (50 mL). The reaction mixture was diluted with water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed with brine solution (2 x 20 mL), dried over ISfeSCU, filtered and evaporated under reduced pressure to get crude residue, which was purified by flash column chromatography (silica gel, 40 g SNAP) using eluents 40% ethyl acetate in heptane to get the desired product as off white solid 326 (390 mg, 52.44%).

LCMS: [M+H] ⁺ : 425.21, Purity= 86.62%.

Synthesis of tert-butyl (R)-(l-((2-((4-(4-morpholino-l-((2-(trimethylsilyl)ethoxy)me thyl)-lH- pyrrolo[3,2-c]pyridin-2-yl)phenyl)carbamoyl)pyridin-4-yl)met hyl)piperidin-3-yl)carbamate (327):

[00910] To a stirred solution of 4-[4-(morpholin-4-yl)-l-{[2-(trimethylsilyl)ethoxy]methyl}-l H- pyrrolo[3,2-c]pyridin-2-yl]aniline 326 (390 mg, 918 pmol) in N,N-dimethylformamide (2 mL) and 4-{[(3R)-3-{[(tert-butoxy)carbonyl]amino}piperidin-l-yl]meth yl}pyridine-2-carboxylic acid 8 (308 mg, 918 pmol) was added DIPEA (0.47 mL, 2.76 mmol) and HATU (524 mg, 1.38 mmol), the resulting reaction mass was stirred at RT for 16h. After completion of reaction (TLC monitoring), the reaction mass was poured into ice-cold water (50 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed with brine solution (2 x 50 mL), dried over Na2SO4, filtered and evaporated under reduced pressure to get crude residue, which was purified by flash column chromatography (silica gel, 12 g SNAP) using eluents 35% ethyl acetate in heptane to get the desired product as yellow solid 327 (390 mg, 43%).

MS: [M+H] ⁺ : 742.63. Synthesis of (R)-4-((3-aminopiperidin-l-yl)methyl)-N-(4-(4-morpholino-lH- pyrrolo[3,2- c]pyridin-2-yl)phenyl)picolinamide (328):

To an ice cold stirred solution of tert-butyl N-[(3R)-l-{[2-({4-[4-(morpholin-4-yl)-l-{[2- (trimethylsilyl)ethoxy]methyl}-lH-pyrrolo[3,2-c]pyridin-2-yl ]phenyl}carbamoyl)pyridin-4-yl]methyl}piperidin-3- yl]carbamate 327 (390 mg, 0.52 mmol) in dichloromethane (2 mL) was added trifluoroacetic acid (1 mL) in dropwise manner . The resulting mixture was stirred at rt for next 2h. After completion of reaction (TLC and LCMS monitoring), solvent was evaporated under reduced pressure to get the crude residue which was triturated with di-ethyl ether (50 mL) to get the desired product as yellow colour solid 328 (250 mg, crude as TFA salt, quantification not done).

LCMS: [M+H] ⁺ : 512.70, Purity= 87.47%.

Synthesis of (R)-4-((3-acrylamidopiperidin-l-yl)methyl)-N-(4-(4-morpholin o-lH-pyrrolo[3,2- c]pyridin-2-yl)phenyl)picolinamide (B0700-614):

[00911] To a stirred solution of 4-{[(3R)-3-aminopiperidin-l-yl]methyl}-N-{4-[4-(morpholin-4- yl)- lH-pyrrolo[3,2-c]pyridin-2-yl]phenyl}pyridine-2-carboxamide 328 (220 mg, 430 pmol) in THF (5 mL) and water (2.5 mL) was added K3PO4 (91.3 mg, 430 pmol). The reaction mixture was stirred at 0°C for 15min under N2 atmosphere. Then added 3-chloropropanoyl chloride 122 (41 pL, 430 pmol) to the reaction mixture drop wise and stirred for next Ih at RT. RM was cooled to 20±5°C, added 2M solution of NaOH over a period of 20 min. RM further stirred for next 2h at 60°C, complete elimination monitored by LCMS. After completion of reaction, the resulting reaction mass was poured into brine solution (100 mL) and aqueous layer was extracted with THF (2x100 mL).

Combined organic layer evaporated under reduced pressure at 45°C, to afford the crude product. The crude was further purified by column chromatography by using silica gel (100-200M ) using eluents 7% MeOH:DCM to get the desired product as yellow solid B0700-614 (30 mg, 12%) .

¹ H NMR (400 MHz, DMSO-d6): 8 11.76 (s, IH), 10.72 (s, IH), 8.69-8.67 (d, IH), 8.11 (s, IH), 8.02- 7.96 (m, 3H), 7.89-7.87 (d, J = 8.40 Hz, 2H), 7.75-7.73 (d, IH), 6.63-6.62 (d, IH), 7.09 (s, IH), 6.89- 6.88 (d, IH), 6.25-6.19 (m, IH), 6.07-6.02 (dd, IH), 5.57-5.54 (dd, IH), 3.84-3.78 (m, 5H), 3.66 (s, 2H), 3.58-3.56 (m, 4H), 3.39 (m, IH), 2.81-2.79 (m, IH), 2.06-2.02 (m, 2H), 1.80-1.74 (m, 2H), 1.61- 1.58 (m, IH) and 1.33-1.28 (m, IH). LCMS: [M+H] ⁺ : 566.44, Purity= 98.31%. Example B0700-66

Synthesis of Compound B0700-615:

Compound was prepared or can be prepared following the synthetic scheme depicted elow and using the general methods and procedures described herein.

Preparation of 4-((l-acryloylpiperidin-4-yl)oxy)-N-(4-(4-morpholino-7H-pyrr olo[2,3- d]pyrimidin-6-yl)phenyl)picolinamide (B0700-615):

Synthesis of tert-butyl 4-((2-cyanopyridin-4-yl)oxy)piperidine-l-carboxylate (331):

[00912] To a stirred suspension of sodium hydride (121 mg, 5.05 mmol) in dimethylformamide (25 mL) was added tert-butyl 4-hydroxypiperidine-l -carboxylate 330 (872 mg, 4.33 mmol) portion wise and the resulting mixture was stirred at rt for 30 min. Then 4-chloropyridine-2-carbonitrile 329 (0.5 g, 3.61 mmol) in dissolved in DMF (5 mL) was added drop wise. The reaction mixture stirred at rt for next 16h. After TLC and LCMS monitoring, the reaction mixture was poured in to ice cold water (50 mL) and extracted with EtOAc (2 X 50 mL). The combined organic layer was dried over Na2SO4 and filtered through cotton plug. Filtrate was concentrate under reduced pressure. Crude was purified by flash column chromatography in silica gel (40 g SNAP) using eluents 2.5% EtOAc in Heptane to get desired product as white solid 331 (0.9 g, 77.28%).

'H NMR (400 MHz, DMSO-d6): 8 8.52-8.50 (d, 1H), 7.75 (s, 1H), 7.33-7.32 (d, 1H), 4.81 (m, 1H), 3.62-3.59 (m, 4), 1.95-1.93 (m, 2H), 1.55-1.51 (m, 2H) and 1.40 (s, 9H). LCMS: [M+H] ⁺ : 304.14, Purity= 94.19%.

Synthesis of 4-((l-(tert-butoxycarbonyl)piperidin-4-yl)oxy)picolinic acid (332):

[00913] To a stirred solution of tert-butyl 4-[(2-cyanopyridin-4-yl)oxy]piperidine-l -carboxylate 331

(0.9 g, 2.97 mmol) in ethanol (18 mL) was added 3N NaOH (2.8 mL). The reaction was continued at 90°C for next 4h. After completion of reaction (TLC monitoring), the reaction mixture was concentrate under reduced pressure. The reaction mixture was diluted with water (20 mL) and acidified pH 5-6 with 3N aq.HCl. Desired product was extracted with 25% IPA in Chloroform (3 x 50 mL). The combined organic layer was dried over ISfeSCU, filtrate and concentrated under reduced pressure. Crude was purified by trituration with diethyl ether to get desired product as transparent viscous liquid 332 (650 mg, 62%).

LCMS: [M+H] ⁺ : 323.21, Purity= 81.19%.

Synthesis of tert-butyl 4-((2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl) -7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)o xy)piperidine-l-carboxylate (333):

[00914] To an ice cold stirred solution of 4-({ l-[(tert-butoxy)carbonyl]piperidin-4-yl}oxy)pyridine- 2-carboxylic acid 332 (636 mg, 1.97 mmol) in dimethylformamide (10 mL) were added 4-[4- (morpholin-4-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyr rolo[2,3-d]pyrimidin-6-yl]aniline 7 (0.7 g, 1.64 mmol), DIPEA (862 pL, 4.93 mmol) and HATU (938 mg, 2.47 mmol) under nitrogen atmosphere. The reaction mixture was stirred at room temperature for next 16h. After completion of reaction (TLC and LCMS monitoring), reaction mixture was poured into cold water (50 mL) and extracted with 25% IPA/ Chloroform (3 x 100 mL). The combined organic layer was washed with sat. brine solution (50 mL), dried over NazSCU and filtered through cotton plug. Filtrate was concentrate under reduced pressure. Crude was purified by flash column chromatography in silica gel (40 g SNAP) using eluents 25% EtOAc in Heptane to get desired product as yellow solid 333 (0.9 g, 70.47%).

^X H NMR (400 MHz, DMSO-d6): 5 10.75 (s, 1H), 8.56-8.54 (d, 1H), 8.27 (s, 1H), 8.06-8.04 (d, J= 7.80 Hz, 2H), 7.78-7.76 (m, 2H), 7.69 (s, 1H), 7.28-7.27 (m, 1H), 6.95 (s, 1H), 5.57 (s, 2H), 4.89 (s, br, 1H), 3.89 (s, 4H), 3.74 (s, 4H), 3.65-3.61 (t, 2H), 3.27 (m, 2H), 1.95 (m, 2H), 1.60-1.57 (m, 2H), 1.41 (s, 9H), 1.25 (m, 1H), 0.89-0.87 (t, 2H) and -0.07 (s, 9H). LCMS: [M+H] ⁺ : 730.34, Purity= 88.06%.

Synthesis of N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)-4-(piperidin-4- yloxy)picolinamide (334):

[00915] To an ice cold stirred solution of tert-butyl 4-{[2-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)pyridin-4- yl]oxy}piperidine-l -carboxylate 334 (0.8 g, 1.1 mmol) in dichloromethane (12 mL) was added trifluoroacetic acid (3 mL) and stirred at room temperature for 2h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure to get crude intermediate. Crude was dissolved in 1,4-di oxane (10 mL) in ethane- 1,2-diamine (3 mL) and stirred at 80°C for next 2h. After completion of reaction (LCMS monitoring), the reaction mixture was poured into ice cold water (80 mL) and extracted with 25% IPA/Chloroform (3 x 100 mL). The combined organic layer was dried over ISfeSCL and filtered through cotton plug. Filtrate was concentrate under reduced pressure to get desired product was brown viscous 334 (425 mg, 70%). LCMS: [M+H] ⁺ : 500.23, Purity= 82%.

Synthesis of 4-((l-acryloylpiperidin-4-yl)oxy)-N-(4-(4-morpholino-7H-pyrr olo[2,3-d]pyrimidin- 6-yl)phenyl)picolinamide (B0700-615):

[00916] To an ice cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-4-(piperidin-4-yloxy)pyridine-2-carboxamide 334 (415 mg, 830 pmol) in dimethylformamide (6.0 mL) was added tri ethylamine (354 pL, 2.52 mmol) and acryloyl chloride 27 (91 mg, 1.01 mmol) in drop wise manner. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS monitoring), reaction mixture was poured into ice water (50 mL) and extracted with 25% IPA/Chloroform(3 X 50 mL). Combined organic layer was dried over Na2SO4, filtered and concentrate under reduced pressure. Crude residue was purified through RP-HPLC purification using 5mM Ammonium Bicarbonate in water/ Acetonitrile (Column: Waters Xselect Phenyl-Hexyl( l 9*250mm,5pm) to get desired product as yellow solid B0700-615 (94 mg, 20%). flT NMR (400 MHz, DMSO-d6): 5 12.19 (s, 1H), 10.71 (s, 1H), 8.56-8.54 (d, J= 8.03 Hz, 1H), 8.18 (s, 1H), 8.00-7.98 (d, J= 8.0 Hz, 2H), 7.92-7.90 (d, J= 8.0 Hz, 2H), 7.70 (d, 1H), 7.30-7.29 (d, 1H), 7.16 (s, 1H), 6.87-6.80 (m, 1H), 6.17-6.09 (dd, 1H), 5.70-5.67 (dd, 1H), 4.98-4.91 (br, 1H), 3.89-3.87 (t, 6H), 3.76-3.74 (t, 4H), 3.53-3.51 (m, 1H), 3.41-3.37 (m, 1H), 2.01 (br, 2H), and 1.64 (br, 2H). LCMS: [M+H] ⁺ : 554.41, Purity= 98.65% Example B0700-67

Synthesis of Compound B0700-616:

Preparation of 4-((l-acryloylpiperidin-4-yl)amino)-N-(4-(4-morpholino-7H-py rrolo [2,3- d]pyrimidin-6-yl)phenyl)picolinamide (B0700-616):

Synthesis of tert-butyl 4-((2-cyanopyridin-4-yl)amino)piperidine-l-carboxylate (335):

[00917] To a stirred solution of tert-butyl 4-aminopiperidine-l -carboxylate 88 (0.8 g, 3.99 mmol) in dimethylformamide (5 mL) was added 4-chloropyridine-2-carbonitrile 329 (553 mg, 3.99 mmol) and K2CO3 (1.66 g, 12 mmol) at RT under N2 atmosphere. The reaction mixture was stirred at 80°C for 16 h. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice- cold water (50 mL) and extracted with ethyl acetate

(2 x 50 mL). The organic layer was washed with brine, dried over ISfeSCU, filtered and concentrated under reduced pressure. Crude was purified by flash column chromatography in silica gel (12 g SNAP) using eluents 44% EtOAc in Heptane to get desired product as yellow solid 335 (450 mg, 32%).

¹ HNMR (400 MHz, DMSO-d6): 58.10-8.08 (d, 1H), 7.10 (s, 1H), 7.06-7.04 (d, J= 8.0 Hz, 1H), 6.75 (s, 1H), 3.89-3.85 (m, 2H), 3.18-3.19 (m, 1H), 2.90 (m, 2H), 1.86-1.83 (m, 2H), 1.40 (s, 9H) and 1.24-1.21 (m, 2H). LCMS: [M+H] ⁺ : 303.18, Purity= 87.12%.

Synthesis of 4-((l-(tert-butoxycarbonyl)piperidin-4-yl)amino)picolinic acid (336):

[00918] To a stirred solution of tert-butyl 4-[(2-cyanopyridin-4-yl)amino]piperidine-l -carboxylate 335 (450 mg, 1.49 mmol) in 1,4-dioxane (6 mL) was added lithium(l+) hydrate hydroxide (749 mg, 17.9 mmol) dissolved in water (3.0 mL) at RT under N2 atmosphere. The reaction mixture was stirred at 90°C for 16h. After completion of reaction (TLC monitoring), the reaction mixture was concentrated under reduced pressure to get crude. Crude was then dissolved in 10% IPA in CHCL and stirred for 15 min. The reaction mixture was filtered through sintered. The filtrate was concentrated under reduced pressured to get the desired product as yellow solid 336 (340 mg).

LCMS: [M+H] ⁺ : 322.17, Purity= 97.64%.

Synthesis of tert-butyl 4-((2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy)methyl) -7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)carbamoyl)pyridin-4-yl)a mino)piperidine-l-carboxylate (337):

[00919] To a stirred solution of 4-({ l-[(tert-butoxy)carbonyl]piperidin-4-yl}amino)pyridine-2- carboxylic acid (340 mg, 1.06 mmol) and 4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 336 (450 mg, 1.06 mmol) in

N,N-dimethylformamide (4 mL) was added HATU (603 mg, 1.59 mmol) at RT under N2 atmosphere. The reaction mixture was stirred for 10 min. Then added DIPEA (410 mg, 3.17 mmol) to the reaction mixture. The resulting reaction mixture was stirred at room temperature for 16h. After completion of reaction (TLC monitoring), the reaction mixture was poured into ice-cold water (30 mL) and extracted with ethyl acetate (2 x 30 mL). The organic layer was washed with brine solution, dried over Na2SO4, filtered and concentrated under reduced pressure. Crude was purified by flash column chromatography in silica gel (12 g SNAP) using eluents 35% Ethyl acetate in Heptane to get desired product as yellow solid 337 (300 mg, 39%).

¹ HNMR (400 MHz, DMSO-d6): 8 10.65 (s, 1H), 8.26 (s, 1H), 8.18-8.17 (d, J= 4.4 Hz, 1H), 8.03-8.01 (d, J= 8.0 Hz, 2H), 7.76-7.74 (d, J= 8.0 Hz, 2H), 7.35 (s, 1H), 6.94-6.92 (d, 2H), 6.74 (s, 1H), 5.57 (s, 2H), 4.02 (m, 6H), 3.89 (m, 5H), 3.73 (t, 2H), 2.98-2.96 (m, 2H), 1.88 (m, 2H), 1.41 (s, 9H), 0.89-

O.87 (t, 2H) and -0.07 (s, 9H). LCMS: [M+H] ⁺ : 729.5, Purity= 82%.

Synthesis of N-(4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)-4-(piperidin-4- ylamino)picolinamide (338):

[00920] To an ice-cold stirred solution of tert-butyl 4-{[2-({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)pyridin-4- yl]amino}piperidine-l -carboxylate 337 (0.3 g, 412 pmol) in dichloromethane (6 mL) was added trifluoroacetic acid (3 mL) under N2 atmosphere. The reaction mixture was stirred at RT for 4h. After LCMS monitoring the reaction mixture was concentrated under reduced pressure to get crude. Crude was then dissolved in 1,4-dioxane (3 mL) was added ethane- 1,2-diamine (447 mg, 7.44 mmol), the reaction mixture was stirred at 80°C for 30 min. After completion of reaction (LCMS monitoring), the reaction mixture was concentrated under reduced pressure, diluted with water (20 mL) and extracted with 10% IPA in CHCL (2 x 20 mL). The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure to get the desired product as brown solid 338 (150 mg, 74%).

LCMS: [M+H] ⁺ : 499.40, Purity= 86%.

Synthesis of 4-((l-acryloylpiperidin-4-yl)amino)-N-(4-(4-morpholino-7H-py rrolo [2,3- d]pyrimidin-6-yl)phenyl)picolinamide (B0700-616):

[00921] To an ice-cold stirred solution ofN-{4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}-4-[(piperidin-4-yl)amino]pyridine-2-carboxamide 338 (150 mg, 501 pmol) in THF (1.00 mL) was added K3PO4 (106 mg, 501 pmol) dissolved in DM Water (500 pL) under N2 atmosphere. The reaction mixture was stirred for 5min. Then 3-chloropropanoyl chloride 122 (21.6 pL, 226 pmol) in THF (1.00 mL) was added dropwise to the reaction mixture and stirred for 15min at RT. After TLC monitoring sodium hydroxide (679 pL, 7.52 mmol) was added to the reaction mixture. The reaction mixture was stirred at RT for Ih. After completion of reaction (LCMS monitoring), the reaction mixture was diluted with water (10 mL) and extracted with 2-methyltetrahydrofuran (3 x 10 mL). The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure to get crude. Crude residue was purified through RP-HPLC purification using eluent as 0.1% Ammonium Hydroxide in water/100%ACN and column using Waters Xtimate Phenyl-Hexyl(19*250mm,10pm) to get desired product as white solid B0700-616 (14 mg, 8%).

^X H NMR (400 MHz, DMSO-d6): 8 12.18 (s, IH), 10.57 (s, IH), 8.18-8.17 (m, 2H), 7.97-7.95 (d, J= 8.0 Hz, 2H), 7.90-7.88 (d, J= 8.0 Hz, 2H), 7.37-7.36 (d, J= 4.0 Hz, IH), 7.15 (s, IH), 6.96-6.97 (d, IH), 6.87-6.80 (m, IH), 6.13-6.08 (dd, IH), 5.69-5.66 (dd, IH), 4.31-4.28 (d, IH), 4.04-4.01 (d, IH), 3.88-3.87 (d, 4H), 3.76-3.74 (d, 5H), 3.30 (t, IH), 2.98-2.93 (m, IH), 1.96-1.94 (m, 2H) and 1.39- 1.29 (br, 2H). LCMS: [M+H] ⁺ : 553.43, Purity= 99.41%.

Example B0700-68

Synthesis of Compound B0700-617:

Preparation of (S)-N-(l-(3-(2-methyl-l-((4-(4-morpholino-7H-pyrrolo[2,3-d]p yrimidin-6- yl)phenyl)amino)-l-oxopropan-2-yl)phenyl)pyrrolidin-3-yl)acr ylamide (B0700-617):

Synthesis of 2-(3-bromophenyl)-2-methyl-N-(4-(4-morpholino-7-((2-

(trimethylsilyl)ethoxy)methyl)-7H-pyrrolo[2,3-d]pyrimidin -6-yl)phenyl)propanamide (341): [00922] To an ice cold stirred solution of 4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 339 (1.5 g, 3.52 mmol) in dimethylformamide (15 mL) was added 2-(3-bromophenyl)-2-methylpropanoic acid 340 (1.11 g, 4.58 mmol), DIPEA (1.85 mL, 10.6 mmol) and HATU (2.01 g, 5.29 mmol) under nitrogen atmosphere. The reaction mixture was stirred at room temperature for next 16h. After completion of reaction (TLC and LCMS monitoring), reaction mixture was poured into ice cold water (40 mL) and extracted with EtOAc (3 x 100 mL). The combined organic layer was washed with sat. brine solution, dried over Na2SO4, filtered and concentrate under reduced pressure. Crude was purified by column chromatography in silica gel (230-400M) using eluents 35% EtOAc/Heptane to get desired product as light brown solid 341 (1.65 g, 69.55%).

¹ HNMR (400 MHz, DMSO-d6): 59.33 (s, 1H), 8.24 (s, 1H), 7.73-7.71 (d, J= 8.0 Hz, 2H), 7.68-7.66 (d, J= 8.0 Hz, 2H), 7.52 (s, 1H), 7.46 (d, 1H), 7.33 (m, 2H), 6.88 (s, 1H), 5.52 (s, 2H), 3.87 (s, 4H), 3.72 (s, 4H), 3.61-3.57 (t, 2H), 1.57 (s, 6H), 0.84-0.80 (t, 2H) and -0.09 (s, 9H). LCMS: [M+H] ⁺ : 650.38, Purity= 96.81%. Synthesis of tert-butyl (S)-(l-(3-(2-methyl-l-((4-(4-morpholino-7-((2-(trimethylsily l) ethoxy) methyl)-7H-pyrrolo[2,3-d] pyrimidin-6-yl)phenyl)amino)-l-oxopropan-2-yl)phenyl) pyrrolidin- 3-yl)carbamate (342):

[00923] To a stirred solution of 2-(3-bromophenyl)-2-methyl-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}propanamide 341 (1.65 g, 2.54 mmol) in toluene (20 mL) was added tert-butyl N-[(3S)-pyrrolidin-3-yl]carbamate 155 (945 mg, 5.07 mmol) and CS2CO3 (2.48 g, 7.61 mmol) at room temperature. The resulting reaction mixture was degassed with argon for next 15 minutes followed by addition of [5- (diphenylphosphanyl)-9,9-dimethyl-9H-xanthen-4-yl]diphenylph osphane (293 mg, 507 pmol) and tris(l,5-diphenylpenta-l,4-dien-3-one) dipalladium (232 mg, 254 pmol). The resulting reaction mixture was stirred at 100°C for next 16h. After the completion of reaction (TLC and LCMS monitoring), the reaction mixture was filtered through celite bed followed by washing with ethyl acetate (2x 100 mL) and mother liquor was washed with water (50 mL). The combined organic layer was washed with brine solution (3 x 50 mL), dried over ISfeSCU filtered through cotton plug. Filtrate was concentrate under reduced pressure. Crude was purified by column chromatography in silica gel (230-400M) using eluents 35%EtOAc/Heptane to get desired product as off white solid 342 (1.7 g, 88.67%).

LCMS: [M+H] ⁺ : 756.31, Purity= 97.71%.

Synthesis of (S)-2-(3-(3-aminopyrrolidin-l-yl)phenyl)-2-methyl-N-(4-(4-mo rpholino-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)propanamide (343):

[00924] To an ice cold stirred solution of tert-butyl N-[(3S)-l-{3-[({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)methyl]phenyl}pyrrolidin-3-yl]carbamate 342 (0.7 g, 962 pmol) in di chloromethane (15 mL) was added trifluoroacetic acid (5 mL), stirred at room temperature for next 3h. The reaction mixture concentrated under reduced pressure. Crude was dissolved in 1,4-di oxane (10 mL) followed by addition of ethane- 1,2-diamine (0.5 mL)and stirred at 80°C for next 2h. After completion of reaction (TLC and LCMS) monitoring, the reaction mixture was poured into ice cold water (50 mL) and extracted with 25% IPA/Chloroform (3 x 50 mL). Combined organic layer was dried over ISfeSCL and filtered through cotton plug. Filtrate was concentrate under reduced pressure to get Crude. Crude was triturated by diethyl ether to get desired product as brown solid 343 (0.5 g, 95%). LCMS: [M+H] ⁺ : 526.21, Purity= 90.72%.

Synthesis of (S)-N-(l-(3-(2-methyl-l-((4-(4-morpholino-7H-pyrrolo [2, 3-d] pyrimidin-6- yl)phenyl)amino)-l-oxopropan-2-yl)phenyl)pyrrolidin-3-yl)acr ylamide (B0700-617):

[00925] To an ice cold stirred solution of 2-{3-[(3S)-3-aminopyrrolidin-l-yl]phenyl}-2-methyl-N- {4-[4-(morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]pheny l}propanamide 343 (0.3 g, 571 pmol) in dimethylformamide (4 mL) was added tri ethylamine (321 pL, 2.28 mmol) and prop-2 - enoyl chloride 27 (51.7 mg, 571 pmol) in drop wise manner. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS) monitoring, The resulting reaction mass was added ice cold water (50 mL) and extracted with 25% IPA/Chloroform (4 X 50 mL). Combined organic layer was dried over Na2SO4 filtered through cotton plug. Filtrate was concentrate under reduced pressure to get crude residue. Crude residue was purified through RP- HPLC purification using eluent as 0.1% Ammonium Hydroxide in water/100%ACN and column using Waters Xtimate Phenyl-Hexyl(19*250mm,10pm) to get desired product as white solid B0700- 617 (101 mg, 30%).

^X H NMR (400 MHz, DMSO-d6): 8 12.11 (s, 1H), 9.08 (s, 1H), 8.38-8.36 (d, J= 8.0 Hz, 2H), 8.16 (s, 1H), 7.82-7.80 (d, J= 8.0 Hz, 2H), 7.68-7.66 (d, J= 8.0 Hz, 2H), 7.16-7.12 (t, 1H), 7.08 (s, 1H), 6.63-6.61 (d, 1H), 6.51 (s, 1H), 6.43-6.41 (d, 1H), 6.25-6.18 (m, 1H), 6.12-6.07 (dd, 1H), 5.59-5.56 (dd, 1H), 4.45-4.41 (m, 1H), 3.87-3.85 (d, 4H), 3.75-3.72 (d, 4H), 3.41-3.38 (m, 1H), 3.32-3.28 (m 2H), 3.11-3.08 (m, 1H), 2.21-2.16 (m, 1H), 1.94-1.88 (m, 1H) and 1.55 (s, 9H). LCMS: [M+H] ⁺ : 580.46, Purity= 98.41%.

Example B0700-69

Synthesis of Compound B0700-618:

Preparation of (S)-N-(l-(3-(2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6- yl)phenyl)amino)-2-oxoethyl)phenyl)pyrrolidin-3-yl)acrylamid e (B0700-618) :

Synthesis of 2-(3-bromophenyl)-N-(4-(4-morpholino-7-((2-(trimethylsilyl)e thoxy)methyl)-7H- pyrrolo [2, 3-d] pyrimidin-6-yl)phenyl)acetamide (345) :

[00926] To an ice cold stirred solution of 4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]aniline 7 (2.5 g, 5.87 mmol) in dimethylformamide (25 mL) was added 2-(3-bromophenyl)acetic acid 344 (1.52 g, 7.05 mmol), DIPEA (3.08 mL, 17.6 mmol) and HATU (3.35 g, 8.81 mmol) under nitrogen atmosphere. The reaction mixture was stirred at room temperature for next 16h. After completion of reaction (TLC and LCMS monitoring), reaction mixture was poured into ice cold water (100 mL) and extracted with EtOAc (3 X 100 mL). The combined organic layer was washed with sat.brine solution, dried over Na2SO4 and filtered through cotton plug. Filtrate was concentrate under reduced pressure to get crude. Crude was purified by column chromatography in silica gel (230-400M) using eluents 45% EtOAc/Heptane to get desired product as off white solid (2.4 g, 57.75%).

^X H NMR (400 MHz, DMSO-d6): 5 10.36 (s, 1H), 8.24 (s, 1H), 7.70 (s, 4H), 7.55 (s, 1H), 7.46-7.45 (d, J= 8.0 Hz, 1H), 7.35-7.28 (m, 2H), 6.88 (s, 1H), 5.52 (s, 2H), 3.87 (s, 4H), 3.72 (s, 4H), 3.32-3.28 (m 2H), 2.68 (s, 2H), 0.85-0.83 (t, 2H), and -0.97 (s, 9H). LCMS: [M+H] ⁺ : 622.31, Purity= 88.79%. Synthesis of tert-butyl (S)-(l-(3-(2-((4-(4-morpholino-7-((2-(trimethylsilyl)ethoxy) methyl)-7H- pyrrolo[2,3-d]pyrimidin-6-yl)phenyl)amino)-2-oxoethyl)phenyl )pyrrolidin-3-yl)carbamate (346):

[00927] To a stirred solution of 2-(3-bromophenyl)-N-{4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}acetamide 345 (1 g, 1.61 mmol) in toluene (10 mL) was added tert-butyl N-[(3S)-pyrrolidin-3-yl]carbamate 155 (598 mg, 3.21 mmol) and CS2CO3 (1.57 g, 4.82 mmol) at room temperature. The resulting reaction mixture was degassed with argon for next 15 minutes followed by addition of [5-(diphenylphosphanyl)-9,9- dimethyl-9H-xanthen-4-yl]diphenylphosphane (186 mg, 321 pmol) and tris(l,5-diphenylpenta-l,4- dien-3-one) dipalladium (147 mg, 161 pmol). The resulting reaction mixture was stirred at 100° C for next 4h. After the completion of reaction (TLC and LCMS monitoring), the reaction mixture was filtered through celite bed followed by washing with ethyl acetate (60 mL). Mother liquor was diluted with water (100 mL) and extracted with ethyl acetate (3 x 50 mL). The combined organic layer was washed with brine solution (2 x 50 mL), dried over ISfeSCU filtered through cotton plug. Filtrate was concentrate under reduced pressure to get crude. Crude was purified by column chromatography in silica gel (230-400M) using eluents 30% EtOAc/Heptane to get desired product as off white solid 346 (950 mg, 68.88%).

LCMS: [M+H] ⁺ : 728.60, Purity= 86.79%.

Synthesis of (S)-2-(3-(3-aminopyrrolidin-l-yl)phenyl)-N-(4-(4-morpholino- 7H-pyrrolo[2,3- d]pyrimidin-6-yl)phenyl)acetamide (347):

[00928] To an ice cold stirred solution of tert-butyl N-[(3S)-l-{3-[({4-[4-(morpholin-4-yl)-7-{[2- (trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidin-6- yl]phenyl}carbamoyl)methyl]phenyl}pyrrolidin-3-yl]carbamate 346 (850 mg, 1.17 mmol) in dichloromethane (20 mL) was added trifluoroacetic acid (8 mL) and stirred at room temperature for 2h. The reaction mixture concentrated under reduced pressure and crude was dissolved 1,4-di oxane (15 mL) followed by addition of ethane- 1,2-diamine (0.5 mL), stirred at 80°C for next 2h. After completion of reaction LCMS monitoring, the reaction mixture was poured in to ice cold water (50 mL) and extracted with 25% IPA/Chloroform (3 X 50 mL). The combined organic layer was dried over Na2SO4 and filtered through cotton plug. Filtrate was concentrate under reduced pressure to get desired product as off white solid 347 (0.7 g).

LCMS: [M+H] ⁺ : 498.32, Purity= 87%. Synthesis of (S)-N-(l-(3-(2-((4-(4-morpholino-7H-pyrrolo[2,3-d]pyrimidin- 6-yl)phenyl)amino)- 2-oxoethyl)phenyl)pyrrolidin-3-yl)acrylamide (B0700-618):

[00929] To an ice cold stirred solution of 2-{3-[(3S)-3-aminopyrrolidin-l-yl]phenyl}-N-{4-[4- (morpholin-4-yl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]phenyl}acet amide 347 (0.3 g, 603 pmol) in dimethylformamide (6 mL) was added triethylamine (339 pL, 2.41 mmol) and prop-2-enoyl chloride 27 (54.6 mg, 603 pmol) in drop wise. The reaction mixture was stirred for 30 min at same temperature. After completion of reaction (TLC and LCMS) monitoring, the resulting reaction mixture was poured into ice cold water (50 mL) and extracted with 25% IPA/Chloroform (3 X 50 mL). The combined organic layer was dried over Na2 SCU. filtered through cotton plug. Filtrate was concentrate under reduced pressure. Crude residue was purified through RP-HPLC purification using eluent as 5mM Ammonium Bicarbonate in water/ Acetonitrile and column using Waters Xselect Phenyl-Hexyl(19*250mm,5pm to get desired product as white solid B0700-618 (52 mg, 15.48%).

'H NMR (400 MHz, DMSO-d6): 8 12.25 (s, 1H), 10.21 (s, 1H), 8.40-8.39 (d, J= 4.0 Hz, 1H), 8.18 (s, 1H), 7.85-7.82 (m, 2H), 7.66-7.64 (d, J= 8.0 Hz, 2H), 7.13-7.07 (m, 2H), 6.61-6.59 (d, 1H), 6.55 (s, 1H), 6.44-6.42 (d, 1H), 6.26-6.19 (m, 1H), 6.12-6.08 (dd, 1H), 5.60-5.571 (dd, 1H), 4.46-4.42 (m, 1H), 3.89-3.86 (d, 4H), 3.76-3.74 (d, 4H), 3.53 (s, 2H), 3.51-3.50 (m, 1H), 3.30 (m, 2H), 3.11-3.10 (m, 1H), 2.23-2.20 (m 1H) and 1.92-1.89 (m, 1H). LCMS: [M+H] ⁺ : 552.39, Purity= 99.58%.

Analytical Data

[00930] Compounds prepared as described herein provided the following NMR and mass spectrometry results.

Additional Exemplary Compounds

[00931] Other compounds provided herein have been or can be prepared according to the synthetic methods, or some variations thereof, described herein. The compounds can be prepared from readily available starting materials using the following general methods and procedures. It will be appreciated that where typical or preferred process conditions (i.e., reaction temperatures, times, mole ratios of reactants, solvents, pressures, etc.) are given; other process conditions can also be used unless otherwise stated. Optimum reaction conditions may vary with the particular reactants or solvent used, but such conditions can be determined by one skilled in the art by routine optimization procedures. [00932] The representative compounds prepared or can be prepared from readily available starting materials using the general methods and procedures described herein are depicted in Table 1.

Table 1: Representative compounds

Example Bl Menin-MLL in vitro Inhibitory Activity

[00933] The present example evaluates the ability of compounds described herein, inhibitors of Menin/MLL interaction, to inhibit cell proliferation. The proliferation inhibitory effect was investigated in human MLL-leukemia cells selected on the bases of MLL fusion proteins and listed in the table below.

[00934] ATP is present in all metabolically active cells and is considered as a marker for cell viability and proliferation. The metabolic cell activity was determined using the CellTiter-Glo kit from Promega, an ATP monitoring system based on the production of luminescence by the reaction of ATP with added UltraGio® recombinant luciferase (Kawano et al., 2016, PLOS One,

8: 1 l(7):e0158888), according to the supplier’s experimental recommendations. The assay is based on a 96 well plate format. All experiments were carried out in duplicate in one test occasion.

[00935] Test items were dissolved at 20 mM in DMSO with purity >99.9% (Sigma catalog no. D8418) and stored at -20°C. Cells were maintained in RPMI-1640 medium (Invitrogen catalog no. 618700) supplemented with 10% of Heat Inactivated FBS (Invitrogen catalog no. 10500) and 1% Pen-Strep (Invitrogen catalog no. 15140) and cultured at 37°C in a humidified incubator with 5% CO2. Cell lines were grown in suspension and the cell density was maintained in a range of 5xl0 ⁴ - IxlO ⁶ viable cells/ml where possible. Cells were pelleted at 130 g x 5 min.

[00936] Serial dilutions of compounds 1 to 3 in DMSO 100% were prepared starting from a 2.5 mM solutions (prepared by diluting 1 :8 the 20mM stock solution) to generate a 7 point concentration response curve (CRC). Seven concentrations of test compounds (e.g., 5.00 x 10' ⁶ - 1.67 x 10' ⁶ - 5.56 x 10' ⁷ - 1.85 x 10' ⁷ - 6.17 x 10' ⁸ - 2.06 x 10' ⁸ - 6.86 x 10' ⁹ M) were assessed in duplicate in an individual test occasion in parallel in the selected cell lines. KO-539 was used as a reference compound and tested in duplicate at seven similar concentrations. 100% of proliferation was represented by the untreated cells (0.2% DMSO).

[00937] Cell proliferation inhibition was monitored at both the following end points: Day 4 (T4) and Day 7 (T7). For the appropriate cell density during the exponential cell growth and re-supply with fresh compound solution at T4, cells were diluted (1 :4) with fresh medium containing the compound concentrati on ( 1 X) .

[00938] For each plate to test, one 0.4 pL copy plate and four 0.3 pL copy plates were stamped into 96-well plates not treated for cell adhesion (Sarstedt - cat. no. 82.1581.001) by acoustic liquid handling (Echo) at a concentration which was 500 fold the final assay concentration. Stamped plates were stored at -20°C.

[00939] On Day 0, the day of the experiment cell line suspensions were counted by Cell Viability Analyser, Vi-CELL (Beckman Coulter AY15292), and diluted with fresh medium to obtain the following cell densities: K-562 500 c/ml, MOLM-13 1,000 c/ml, MV4-11 10,000 c/ml, OCI-AML-3 500 c/ml, and U-937 500 - 1,000 c/ml. 200 pL/well and 150 pL/well of cell suspension were added into the 0.4pL/well and 0.3pL/well compound plates, respectively. Cell plate containing 200pL/well suspension were incubated at 37°C in a humidified incubator with 5% CO2. From each well of the 150 pL/well cell assay plate, lOOpL were harvested and transferred into a 96-well Optiplate (Perkin Elmer, cat. no. 6005290). Cell viability was measured as described below.

[00940] On Day 4, 150pL/well of fresh medium was added into a new 0.3 pL/well copy compound plates. From each well of the 200pL/well cell assay plate, 100 pL was sampled for the cell viability measurement as described below. 50pL was harvested and added to the 150pL/well compound plate prepared as described in the first point to dilute 1 :4 the cell suspension. The cell assay plate diluted and containing 200 pL/well suspension was incubated at 37°C in a humidified incubator with 5% CO2.

[00941] On Day 4, 150pL/well of fresh medium was added into a new 0.3 pL/well copy compound plates. From each well of the 200pL/well cell assay plate, 100 pL was sampled for the cell viability measurement as described below.

[00942] Cell viability measurement. Plates containing the samples to be tested were equilibrated at room temperature for approximately 30 min and then 30 pL/well of the Promega CellTiterGlo® reagent (Promega catalog no. G7572) were added. Contents were mixed for 5 min on an orbital shaker to induce cell lysis and then incubated at room temperature for an additional 10 min to stabilize the luminescent signal (in the dark). Luminescence was read by using VictorV (Perkin Elmer) multilabel plate reader using the luminescence for 96 well plate standard protocol.

[00943] Data handling. Data was expressed as % of inhibition compared to the 0.2% DMSO negative control, and is calculated as follows: % inhibition =100-[(RLLT sample) x 100/(RLLT average controls)]. CRCs were analysed by GraphPad (Prism) and IC50 values were be calculated by non-linear regression using a 4 parameter-logistic equation. IC50 (pM) values are reported in the table below.

[00944] Compounds of the disclosure provided the following IC50 values:

Example B2: Pharmaceutical Compositions

[00945] The compositions described below are presented with a compound of Formula (L-I), (L-II), and (I) for illustrative purposes.

Example B2a: Parenteral Composition

[00946] To prepare a parenteral pharmaceutical composition suitable for administration by injection, 100 mg of a water-soluble salt of a compound of Formula (L-I), (L-II), and (I) is dissolved in DMSO and then mixed with 10 mL of 0.9% sterile saline. The mixture is incorporated into a dosage unit form suitable for administration by injection.

Example B2b: Oral Composition

[00947] To prepare a pharmaceutical composition for oral delivery, 100 mg of a compound of Formula (L-I), (L-II), and (I) is mixed with 750 mg of starch. The mixture is incorporated into an oral dosage unit for, such as a hard gelatin capsule, which is suitable for oral administration.

Example B2c: Sublingual (Hard Lozenge) Composition

[00948] To prepare a pharmaceutical composition for buccal delivery, such as a hard lozenge, mix 100 mg of a compound of Formula (L-I), (L-II), and (I) with 420 mg of powdered sugar mixed, with 1.6 mL of light com syrup, 2.4 mL distilled water, and 0.42 mL mint extract. The mixture is gently blended and poured into a mold to form a lozenge suitable for buccal administration. Example Bld: Inhalation Composition

[00949] To prepare a pharmaceutical composition for inhalation delivery, 20 mg of a compound of Formula (L-I), (L-II), and (I) is mixed with 50 mg of anhydrous citric acid and 100 mL of 0.9% sodium chloride solution. The mixture is incorporated into an inhalation delivery unit, such as a nebulizer, which is suitable for inhalation administration.

Example B2e: Rectal Gel Composition

[00950] To prepare a pharmaceutical composition for rectal delivery, 100 mg of a compound of Formula (L-I), (L-II), and (I) is mixed with 2.5 g of methylcelluose (1500 mPa), 100 mg of methylparapen, 5 g of glycerin and 100 mL of purified water. The resulting gel mixture is then incorporated into rectal delivery units, such as syringes, which are suitable for rectal administration.

Example B2f: Topical Gel Composition

[00951] To prepare a pharmaceutical topical gel composition, 100 mg of a compound of Formula (L- I), (L-II), and (I) is mixed with 1.75 g of hydroxypropyl celluose, 10 mL of propylene glycol, 10 mL of isopropyl myristate and 100 mL of purified alcohol USP. The resulting gel mixture is then incorporated into containers, such as tubes, which are suitable for topicl administration.

Example B2g: Ophthalmic Solution Composition

[00952] To prepare a pharmaceutical opthalmic solution composition, 100 mg of a compound of Formula (L-I), (L-II), and (I) is mixed with 0.9 g of NaCl in 100 mL of purified water and filterd using a 0.2 micron filter. The resulting isotonic solution is then incorporated into ophthalmic delivery units, such as eye drop containers, which are suitable for ophthalmic administration.

[00953] It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein are hereby incorporated by reference in their entirety for all purposes.

[00954] At least some of the chemical names of compounds provided herein as given and set forth in this application, may have been generated on an automated basis by use of a commercially available chemical naming software program, and have not been independently verified In the instance where the indicated chemical name and the depicted structure differ, the depicted structure will control. In the chemical structures where a chiral center exists in a structure but no specific stereochemistry is shown for the chiral center, both enantiomers associated with the chiral structure are encompassed by the structure.