Title:
FUSION PRIMER FOR THIRD-GENERATION SEQUENCING LIBRARY CONSTRUCTION, AND LIBRARY CONSTRUCTION METHOD, SEQUENCING METHOD AND LIBRARY CONSTRUCTION KIT THEREFOR
Document Type and Number:
WIPO Patent Application WO/2020/164015
Kind Code:
A1
Abstract:
Disclosed are a fusion primer for third-generation sequencing library construction, and a library construction method, a sequencing method and a library construction kit therefor. The fusion primer, from the 5' end to the 3' end thereof, sequentially comprises a homologous recombination sequence, a tag sequence and a specific amplification primer sequence, wherein the homologous recombination sequence is used for performing homologous recombination splicing on amplification products of the fusion primer; the tag sequence is used for distinguishing different amplification products; and the specific amplification primer sequence is used for binding to a target sequence in order to perform primer extension. By means of the amplification of the target sequence by using the fusion primer, and by means of performing homologous recombination splicing on the amplification products, the number of samples on a sequencing chip is increased and the cost of sequencing is reduced.
Inventors:
HUANG BIAO (CN)
HUANG JIN (CN)
DING FEN (CN)
ZHU HUANWEN (CN)
TIAN ZHIJIAN (CN)
HUANG JIN (CN)
DING FEN (CN)
ZHU HUANWEN (CN)
TIAN ZHIJIAN (CN)
Application Number:
PCT/CN2019/074977
Publication Date:
August 20, 2020
Filing Date:
February 13, 2019
Export Citation:
Assignee:
WUHAN BGI CLINICAL LABORATORY CO LTD (CN)
International Classes:
C12N15/10; C40B50/06
Foreign References:
| CN104293783A | 2015-01-21 | |||
| CN106754870A | 2017-05-31 | |||
| CN108070643A | 2018-05-25 | |||
| CN107829146A | 2018-03-23 | |||
| CN109136222A | 2019-01-04 |
Other References:
WANG, B.: "Unveiling the complexity of the maize transcriptome by sing- le-molecule long-read sequencing", NATURE COMMUNICATIONS, 24 June 2016 (2016-06-24), XP055733535
SCHLOSS, P.D.: "Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system", PEERJ., 28 March 2016 (2016-03-28), XP055733544
SCHLOSS, P.D.: "Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system", PEERJ., 28 March 2016 (2016-03-28), XP055733544
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
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