Title:
GCGR ANTIBODY AND GLP-1 FUSION PROTEIN THEREOF, PHARMACEUTICAL COMPOSITION THEREOF AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/196603
Kind Code:
A1
Abstract:
Provided herein are a GCGR antibody, a GLP-1 fusion protein thereof, and a pharmaceutical composition thereof. Further provided herein is a method for using the GCGR antibody and the GLP-1 fusion protein thereof for treating, preventing or ameliorating one or more symptoms of hyperglycemia, type 2 diabetes, metabolic syndrome, or dyslipidemia.
Inventors:
ZHANG CHENG (CN)
ZHANG HUA (CN)
WANG XIAOFENG (CN)
YAO CHENJIANG (CN)
JIANG YAN (CN)
BI LIANGLIANG (CN)
JING SHUQIAN (CN)
ZHANG HUA (CN)
WANG XIAOFENG (CN)
YAO CHENJIANG (CN)
JIANG YAN (CN)
BI LIANGLIANG (CN)
JING SHUQIAN (CN)
Application Number:
PCT/CN2019/078674
Publication Date:
October 17, 2019
Filing Date:
March 19, 2019
Export Citation:
Assignee:
GMAX BIOPHARM LLC (CN)
International Classes:
C07K16/26; A61K39/395; A61P3/10; C07K14/605; C12N15/13
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LEE ET AL., DIABETOLOGIA, vol. 59, pages 1372 - 5
HOLLINGERHUDSON, NATURE BIOTECHNOLOGY, vol. 23, 2005, pages 1126 - 1136
HARRIS, JOURNAL OF CHROMATOGRAPHY, vol. 705, 1995, pages 129 - 134
BAINES ET AL.: "Methods in Molecular Biology", vol. 10, 1992, THE HUMANA PRESS, INC., article "Purification of Immunoglobulin G (IgG", pages: 79 - 104
THOMPSON ET AL., J. MOL. BIOL., vol. 256, 1996, pages 350 - 368
URLAUB ET AL., PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216 - 20
HUSTON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 5879
YANG ET AL., J. MOL. BIOL., vol. 254, 1995, pages 392 - 403
MARKS ET AL., BIOLTECHNOLOGY, vol. 10, 1992, pages 779 - 783
PATTEN ET AL., CURR. OPIN. BIOTECHNOL., vol. 8, 1997, pages 724 - 733
VAUGHAN ET AL., NATURE BIOTECHNOLOGY, vol. 16, 1998, pages 535 - 539
LUNDE ET AL., BIOCHEM. SOC. TRANS., vol. 30, 2002, pages 500 - 06
KORTT ET AL., PROT. ENG., vol. 10, 1997, pages 423
KRIANGKUM ET AL., BIOMOL. ENG., vol. 18, 2001, pages 31 - 108
DE GRAAF ET AL., METHODS MOL. BIOL., vol. 178, 2002, pages 379 - 87
NISONOFFET ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 89, 1960, pages 230
PORTER, BIOCHEM. J., vol. 73, 1959, pages 119
EDELMAN ET AL.: "Methods in Enzymology", vol. 1, 1967, ACADEMIC PRESS, pages: 422
ANDREWS, S.M.TITUS, J. A. ET AL.: "Current Protocols in Immunology", 2003, JOHN WILEY & SONS, pages: 2.8.1 - 2.8.10,2.10A.1-2.10A.5
LARRICK ET AL., METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, vol. 2, 1991, pages 106
COURTENAY-LUCK ET AL.: "Monoclonal Antibodies: Production, Engineering and Clinical Application", 1995, CAMBRIDGE UNIVERSITY PRESS, article "Genetic Manipulation of Monoclonal Antibodies", pages: 166
WARD ET AL.: "Monoclonal Antibodies: Principles and Applications", 1995, WILEY-LISS, INC., article "Genetic Manipulation and Expression or Antibodies"
WALDER ET AL., GENE, vol. 42, 1986, pages 133
BAUER ET AL., GENE, vol. 37, 1985, pages 73
CRAIK, BIOTECHNIQUES, vol. 3, 1985, pages 12 - 19
SMITH ET AL.: "Genetic Engineering: Principles and Methods", 1981, PLENUM PRESS
HOPP ET AL., BIO/TECHNOLOGY, vol. 6, 1988, pages 1204
ASHKENAZI ET AL., PNAS USA, vol. 88, 1991, pages 10535
HOLLENBAUGH ET AL.: "Construction of Immunoglobulin Fusion Proteins", CURRENT PROTOCOLS IN IMMUNOLOGY, 1992, pages 10.19.1 - 10.19.11
BAUM ET AL., EMBO J., vol. 13, 1994, pages 3992 - 4001
HOPPE ET AL., FEBS LETTERS, vol. 344, 1994, pages 191
FANSLOW ET AL., SEMIN. IMMUNOL., vol. 6, 1994, pages 267 - 78
MOULT, CURR. OP. BIOTECH., vol. 7, 1996, pages 422 - 427
CHOU ET AL., BIOCHEMISTRY, vol. 113, 1974, pages 211 - 222
CHOU ET AL., ADV. ENZYMOL. RELAT. AREAS MOL. BIOL., vol. 47, 1978, pages 45 - 148
CHOU ET AL., ANN. REV. BIOCHEM., vol. 47, 1979, pages 251 - 276
CHOU ET AL., BIOPHYS. J., vol. 26, pages 367 - 384
HOLM ET AL., NUCL. ACID. RES., vol. 27, 1999, pages 244 - 247
BRENNER ET AL., CURR. OP. STRUCT. BIOL., vol. 7, 1997, pages 369 - 376
JONES, CURR. OPIN. STRUCT. BIOL., vol. 7, 1997, pages 377 - 87
SIPPL ET AL., STRUCTURE, vol. 4, 1996, pages 15 - 19
BOWIE ET AL., SCIENCE, vol. 253, 1991, pages 164 - 170
GRIBSKOV ET AL., METH. ENZYM., vol. 183, 1990, pages 146 - 159
GRIBSKOV ET AL., PROC. NAT. ACAD. SCI., vol. 84, 1987, pages 4355 - 4358
"Proteins, Structures and Molecular Principles", 1984, W. H. FREEMAN AND COMPANY
MCMAHAN ET AL., EMBO J., vol. 10, 1991, pages 2821
NYGRENUHLEN, CURRENT OPINION IN STRUCTURAL BIOLOGY, vol. 7, 1997, pages 463 - 469
SAMBROOKFRITSCHMANIATIS: "Current Protocols in Molecular Biology", 1995, COLD SPRING HARBOR LABORATORY PRESS, pages: 6.3.1 - 6.3.6
VOSS ET AL., TRENDS BIOCHEM. SCI., vol. 11, 1986, pages 287
MANIATIS ET AL., SCIENCE, vol. 236, 1987, pages 1237
RASMUSSEN ET AL., CYTOTECHNOLOGY, vol. 28, 1998, pages 31
POUWELS ET AL.: "Cloning Vectors: A Laboratory Manual", 1985, ELSEVIER
"Remington's Pharmaceutical Sciences", 1980, MACK PUBLISHING COMPANY
LI ET AL., BIOCHEM PHARMACOL., vol. 150, 2018, pages 46 - 53
See also references of EP 3778634A4
WAKELAM ET AL., NATURE, vol. 323, 1986, pages 68 - 71
PITTNERFAIN, BIOCHEM J., vol. 277, 1991, pages 371 - 8
BRAND ET AL., DIABETES, vol. 45, 1996, pages 1076
KOSTIC ET AL., DIABETES OBES METAB, vol. 20, 2018, pages 283 - 91
GU ET AL., JPET, vol. 331, 2009, pages 871 - 881
MARI, DIABETES OBES METAB, vol. 18, 2016, pages 834 - 9
KORNDORFER ET AL., PROTEINS, vol. 53, 2003, pages 121 - 129
ROQUE ET AL., BIOTECHNOL. PROG., vol. 20, 2004, pages 639 - 654
WARD ET AL., NATURE, vol. 334, 1989, pages 544 - 546
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, U.S. DEPT. OF HEALTH AND HUMAN SERVICES, PHS, NIH, NIH, article "Publication No. 91-3242"
LANDSCHULZ ET AL., SCIENCE, vol. 240, 1988, pages 1759 - 426
HUSTON ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 85, 1988, pages 5879 - 83
HOLLIGER ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 90, 1993, pages 6444 - 48
POLJAK ET AL., STRUCTURE, vol. 2, 1994, pages 1121 - 23
BYRN ET AL., NATURE, vol. 344, 1990, pages 677
BARON ET AL., NUCLEIC ACIDS RES., vol. 23, pages 3605 - 06
JELINEK ET AL., SCIENCE, vol. 259, 1993, pages 1614 - 16
AHREN ET AL., PEPTIDES, vol. 67, 2015, pages 74 - 81
THORNTON ET AL., NATURE, vol. 354, 1991, pages 105 - 291
LEE ET AL., DIABETOLOGIA, vol. 59, pages 1372 - 5
HOLLINGERHUDSON, NATURE BIOTECHNOLOGY, vol. 23, 2005, pages 1126 - 1136
HARRIS, JOURNAL OF CHROMATOGRAPHY, vol. 705, 1995, pages 129 - 134
BAINES ET AL.: "Methods in Molecular Biology", vol. 10, 1992, THE HUMANA PRESS, INC., article "Purification of Immunoglobulin G (IgG", pages: 79 - 104
THOMPSON ET AL., J. MOL. BIOL., vol. 256, 1996, pages 350 - 368
URLAUB ET AL., PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216 - 20
HUSTON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 5879
YANG ET AL., J. MOL. BIOL., vol. 254, 1995, pages 392 - 403
MARKS ET AL., BIOLTECHNOLOGY, vol. 10, 1992, pages 779 - 783
PATTEN ET AL., CURR. OPIN. BIOTECHNOL., vol. 8, 1997, pages 724 - 733
VAUGHAN ET AL., NATURE BIOTECHNOLOGY, vol. 16, 1998, pages 535 - 539
LUNDE ET AL., BIOCHEM. SOC. TRANS., vol. 30, 2002, pages 500 - 06
KORTT ET AL., PROT. ENG., vol. 10, 1997, pages 423
KRIANGKUM ET AL., BIOMOL. ENG., vol. 18, 2001, pages 31 - 108
DE GRAAF ET AL., METHODS MOL. BIOL., vol. 178, 2002, pages 379 - 87
NISONOFFET ET AL., ARCH. BIOCHEM. BIOPHYS., vol. 89, 1960, pages 230
PORTER, BIOCHEM. J., vol. 73, 1959, pages 119
EDELMAN ET AL.: "Methods in Enzymology", vol. 1, 1967, ACADEMIC PRESS, pages: 422
ANDREWS, S.M.TITUS, J. A. ET AL.: "Current Protocols in Immunology", 2003, JOHN WILEY & SONS, pages: 2.8.1 - 2.8.10,2.10A.1-2.10A.5
LARRICK ET AL., METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, vol. 2, 1991, pages 106
COURTENAY-LUCK ET AL.: "Monoclonal Antibodies: Production, Engineering and Clinical Application", 1995, CAMBRIDGE UNIVERSITY PRESS, article "Genetic Manipulation of Monoclonal Antibodies", pages: 166
WARD ET AL.: "Monoclonal Antibodies: Principles and Applications", 1995, WILEY-LISS, INC., article "Genetic Manipulation and Expression or Antibodies"
WALDER ET AL., GENE, vol. 42, 1986, pages 133
BAUER ET AL., GENE, vol. 37, 1985, pages 73
CRAIK, BIOTECHNIQUES, vol. 3, 1985, pages 12 - 19
SMITH ET AL.: "Genetic Engineering: Principles and Methods", 1981, PLENUM PRESS
HOPP ET AL., BIO/TECHNOLOGY, vol. 6, 1988, pages 1204
ASHKENAZI ET AL., PNAS USA, vol. 88, 1991, pages 10535
HOLLENBAUGH ET AL.: "Construction of Immunoglobulin Fusion Proteins", CURRENT PROTOCOLS IN IMMUNOLOGY, 1992, pages 10.19.1 - 10.19.11
BAUM ET AL., EMBO J., vol. 13, 1994, pages 3992 - 4001
HOPPE ET AL., FEBS LETTERS, vol. 344, 1994, pages 191
FANSLOW ET AL., SEMIN. IMMUNOL., vol. 6, 1994, pages 267 - 78
MOULT, CURR. OP. BIOTECH., vol. 7, 1996, pages 422 - 427
CHOU ET AL., BIOCHEMISTRY, vol. 113, 1974, pages 211 - 222
CHOU ET AL., ADV. ENZYMOL. RELAT. AREAS MOL. BIOL., vol. 47, 1978, pages 45 - 148
CHOU ET AL., ANN. REV. BIOCHEM., vol. 47, 1979, pages 251 - 276
CHOU ET AL., BIOPHYS. J., vol. 26, pages 367 - 384
HOLM ET AL., NUCL. ACID. RES., vol. 27, 1999, pages 244 - 247
BRENNER ET AL., CURR. OP. STRUCT. BIOL., vol. 7, 1997, pages 369 - 376
JONES, CURR. OPIN. STRUCT. BIOL., vol. 7, 1997, pages 377 - 87
SIPPL ET AL., STRUCTURE, vol. 4, 1996, pages 15 - 19
BOWIE ET AL., SCIENCE, vol. 253, 1991, pages 164 - 170
GRIBSKOV ET AL., METH. ENZYM., vol. 183, 1990, pages 146 - 159
GRIBSKOV ET AL., PROC. NAT. ACAD. SCI., vol. 84, 1987, pages 4355 - 4358
"Proteins, Structures and Molecular Principles", 1984, W. H. FREEMAN AND COMPANY
MCMAHAN ET AL., EMBO J., vol. 10, 1991, pages 2821
NYGRENUHLEN, CURRENT OPINION IN STRUCTURAL BIOLOGY, vol. 7, 1997, pages 463 - 469
SAMBROOKFRITSCHMANIATIS: "Current Protocols in Molecular Biology", 1995, COLD SPRING HARBOR LABORATORY PRESS, pages: 6.3.1 - 6.3.6
VOSS ET AL., TRENDS BIOCHEM. SCI., vol. 11, 1986, pages 287
MANIATIS ET AL., SCIENCE, vol. 236, 1987, pages 1237
RASMUSSEN ET AL., CYTOTECHNOLOGY, vol. 28, 1998, pages 31
POUWELS ET AL.: "Cloning Vectors: A Laboratory Manual", 1985, ELSEVIER
"Remington's Pharmaceutical Sciences", 1980, MACK PUBLISHING COMPANY
LI ET AL., BIOCHEM PHARMACOL., vol. 150, 2018, pages 46 - 53
See also references of EP 3778634A4
Attorney, Agent or Firm:
AFD CHINA INTELLECTUAL PROPERTY LAW OFFICE (CN)
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