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Patent Searching and Data


Title:
GENE ANALYSIS METHOD, AND DEVICE AND KIT FOR ANALYSIS OF GENE
Document Type and Number:
WIPO Patent Application WO/2014/061146
Kind Code:
A1
Abstract:
A DNA sequencer is a device for analyzing a nucleotide sequence, and is not intended for the detection of a polymorphism that occurs in a trace amount. Furthermore, the crosstalk at the wavelength of a fluorescent substance to be used for the labeling of DNA interferes with the acquisition of low signals on a single coordinate. Therefore, it has been difficult to detect the mutation of a gene having a small abundance ratio, such as the mutation in a somatic cell. A gene analysis device is equipped with: multiple flow paths which respectively correspond to nucleotide species in a nucleic acid sample in which the nucleotides are labeled separately; a wave form data generation section for detecting a label signal from each of the nucleotides in the nucleic acid sample that is placed in each of the multiple flow paths, thereby generating wave form data on the intensity of the detected signal; a peak detection section for detecting a peak value of the wave form data for each of the nucleotides; and a data integration section for carrying out an integration processing of multiple sets of wave form data.

Inventors:
YOKOI TAKAHIDE (JP)
ANAZAWA TAKASHI (JP)
Application Number:
PCT/JP2012/077029
Publication Date:
April 24, 2014
Filing Date:
October 19, 2012
Export Citation:
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Assignee:
HITACHI LTD (JP)
International Classes:
G01N27/447; C12M1/00; C12N15/09; C12Q1/68
Foreign References:
JPH09318600A1997-12-12
JPH07270378A1995-10-20
JPS61269066A1986-11-28
JPH0593712A1993-04-16
Attorney, Agent or Firm:
INOUE Manabu et al. (JP)
Manabu Inoue (JP)
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