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Title:
GENE MARKER LINKED TO GENETIC SEX OF YELLOWTAIL, SEX DETERMINATION METHOD FOR YELLOWTAIL, AND PRIMER FOR USE IN SEX DETERMINATION METHOD
Document Type and Number:
WIPO Patent Application WO2010110373
Kind Code:
A1
Abstract:
Genomic DNA from a yellowtail is subjected to a PCR method or a nucleic acid amplification reaction method using an yellowtail-derived nucleic acid as a template, wherein the PCR method or the nucleic acid amplification reaction method is carried out using, as primers, oligonucleotides comprising the nucleotide sequences: 5'-TTTCATTGTGGCGCTCAG-3' and 5'-GGTTGTAATGTGTCCCAG-3' both of which are produced based on a gene marker for genetic linkage group (LG) 12 in an yellowtail genetic linkage map. A product of the method is subjected to a gel electrophoresis method to analyze the product. In this manner, the genetic sex of the yellowtail can be determined. This technique enables the determination of the genetic sex of an immature yellowtail with reliability without killing the yellowtail.

Inventors:
SAKAMOTO TAKASHI (JP)
FUJI KANAKO (JP)
OZAKI AKIYUKI (JP)
OKAUCHI MASANORI (JP)
ARAKI KAZUO (JP)
HATTORI KEITA (JP)
YOSHIDA KAZUNORI (JP)
Application Number:
PCT/JP2010/055236
Publication Date:
September 30, 2010
Filing Date:
March 25, 2010
Export Citation:
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Assignee:
UNIV TOKYO NAT UNIV CORP (JP)
INC ADMINISTRATIVE AGENCY FISH (JP)
SAKAMOTO TAKASHI (JP)
FUJI KANAKO (JP)
OZAKI AKIYUKI (JP)
OKAUCHI MASANORI (JP)
ARAKI KAZUO (JP)
HATTORI KEITA (JP)
YOSHIDA KAZUNORI (JP)
International Classes:
C12N15/09; C12Q1/68
Foreign References:
JP2008237129A2008-10-09
Other References:
OHARA, E. ET AL.: "Genetic linkage maps of two yellowtails(Seriola quinqueradiata and Seriola lalandi)", AQUACULTURE, vol. 244, 2005, pages 41 - 48
HIROYASU SATO ET AL.: "Heisei 20 nendo Abstracts for the spring meeting of the Japanese Society of Fisheries Science", 2008, article "Buri Microsatellite Marker no Kaihatsu", pages: 247
KAZUO ARAKI: "Tokushu Suisan Yoshoku ni okeru Iden Ikushu Bunya no Sentan Gijutsu Gyorui ni okeru Bunshi Seibutsugakuteki Kenkyu no Genjo", RESEARCH JOURNAL OF FOOD AND AGRICULTURE, vol. 15, no. 2, 1992, pages 21 - 25
Attorney, Agent or Firm:
AIWA INTERNATIONAL PATENT AGENCY (JP)
It suits and is わ特許業務法人. (JP)
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