Title:
GENES AND PROTEINS INVOLVED IN THE BIOSYNTHESIS OF ENEDIYNE RING STRUCTURES
Document Type and Number:
WIPO Patent Application WO2002094861
Kind Code:
A3
Abstract:
Five protein families cooperate to form the warhead structure that characterizes enediyne compounds, both chromoprotein enediynes and non-chromoprotein enediynes. The protein families include a polyketide synthase and thioesterase protein which form a polyketide synthase catalytic complex involved in warhead formation in enediynes. Genes encoding a member of each of the five protein families are found in all enediyne biosynthetic loci. The genes and proteins may be used in genetic engineering applications to design new enediyne compounds and in methods to identify new enediyne biosynthetic loci.
Inventors:
FARNET CHRIS M (CA)
ZAZOPOULOS EMMANUEL (CA)
STAFFA ALFREDO (CA)
ZAZOPOULOS EMMANUEL (CA)
STAFFA ALFREDO (CA)
Application Number:
PCT/CA2002/000744
Publication Date:
July 03, 2003
Filing Date:
May 21, 2002
Export Citation:
Assignee:
ECOPIA BIOSCIENCES INC (CA)
FARNET CHRIS M (CA)
ZAZOPOULOS EMMANUEL (CA)
STAFFA ALFREDO (CA)
FARNET CHRIS M (CA)
ZAZOPOULOS EMMANUEL (CA)
STAFFA ALFREDO (CA)
International Classes:
C12N15/09; A61K31/27; A61K31/4748; A61K31/7048; A61P35/00; A61P35/02; C07D491/08; C07H17/04; C07H17/08; C07K14/36; C07K16/40; C12N9/00; C12N15/31; C12N15/52; C12P21/02; C12Q1/26; C12Q1/68; G06F17/30; (IPC1-7): C12N15/52; C07K14/36; C07K16/40; C12N15/31; C12Q1/68
Domestic Patent References:
| WO2000040596A1 | 2000-07-13 | |||
| WO2000037608A2 | 2000-06-29 |
Foreign References:
| US4259319A | 1981-03-31 |
Other References:
WEN LIU ET AL.: "Genes for production of the enedyine antitumor antibiotic C-1027 in Streptomyces globisporus are clustered with the cagA gene that encodes the C-1027 apoprotein", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 44, no. 2, February 2000 (2000-02-01), pages 382 - 392, XP002926776
JON S. THORSON ET AL.: "Enedyine biosynthesis and self-resistance: A progress report", BIOORGANIC CHEMISTRY, vol. 27, no. 2, 1999, pages 172 - 188, XP002222216
JON S. THORSON ET AL.: "Enedyine biosynthesis and self-resistance: A progress report", BIOORGANIC CHEMISTRY, vol. 27, no. 2, 1999, pages 172 - 188, XP002222216
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