Title:
GENETICALLY ENGINEERED BACTERIA AND APPLICATION THEREOF, AND METHOD FOR PRODUCING PROSTAGLANDIN E2
Document Type and Number:
WIPO Patent Application WO/2021/120566
Kind Code:
A1
Abstract:
Provided are a recombinant Escherichia coli and an application thereof in producing prostaglandin E2. The recombinant Escherichia coli overexpresses a coding gene of prostaglandin H synthase and a coding gene of prostaglandin E synthase. Also provided is a method for producing prostaglandin E2, in which arachidonic acid is used as a substrate to produce prostaglandin E2 using the recombinant Escherichia coli.
Inventors:
WANG WEI (CN)
WANG KANGLIN (CN)
YUAN ZHECHENG (CN)
JIN YONGHONG (CN)
WANG QITONG (CN)
JIA HEPING (CN)
HU ZHIHAO (CN)
WANG KANGLIN (CN)
YUAN ZHECHENG (CN)
JIN YONGHONG (CN)
WANG QITONG (CN)
JIA HEPING (CN)
HU ZHIHAO (CN)
Application Number:
PCT/CN2020/097211
Publication Date:
June 24, 2021
Filing Date:
June 19, 2020
Export Citation:
Assignee:
HEFEI KNATURE BIO PHARM CO LTD (CN)
International Classes:
C12N1/21; C12N9/02; C12N9/90; C12N15/53; C12N15/61; C12N15/70; C12P31/00; C12R1/19
Domestic Patent References:
| WO2007104019A2 | 2007-09-13 | |||
| WO2007104019A2 | 2007-09-13 |
Foreign References:
| CN110951814A | 2020-04-03 | |||
| US7169580B1 | 2007-01-30 | |||
| US7169580B1 | 2007-01-30 |
Other References:
DATABASE NUCLEOTIDE 19 April 2014 (2014-04-19), ANONYMOUS: "Synthetic construct prostaglandin endoperoxide H synthase gene, complete cds", XP055822174, retrieved from GENBANK Database accession no. KJ415281
DATABASE PROTEIN 1 October 2019 (2019-10-01), ANONYMOUS: "prostaglandin E synthase [Homo sapiens]", XP055822585, retrieved from GENBANK Database accession no. NP_004869
DATABASE NUCLEOTIDE 24 February 2019 (2019-02-24), ANONYMOUS: "Macaca fascicularis prostaglandin E synthase 2 (PTGES2), mRNA", XP055822591, retrieved from GENBANK Database accession no. NM_001284110
DATABASE PROTEIN 1 October 2019 (2019-10-01), ANONYMOUS: "prostaglandin E synthase [Homo sapiens]", XP055822585, retrieved from GENBANK Database accession no. NP_004869
DATABASE NUCLEOTIDE 24 February 2019 (2019-02-24), ANONYMOUS: "Macaca fascicularis prostaglandin E synthase 2 (PTGES2), mRNA", XP055822591, retrieved from GENBANK Database accession no. NM_001284110
Attorney, Agent or Firm:
CHENGGUO INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
Download PDF: