Title:
GENOME EDITING METHOD
Document Type and Number:
WIPO Patent Application WO/2020/111028
Kind Code:
A1
Abstract:
A method in which gold particles are coated with protein, the method being characterized by using either of the following buffering solutions. 1) A buffering solution that has a final salt concentration, excluding MgCl2 and DTT, of greater than 0 mM but no greater than 3.5 mM. 2) A buffering solution that does not include a salt.
Inventors:
HAMADA HARUYASU (JP)
NAGIRA YOZO (JP)
MIKI RYUJI (JP)
TAOKA NAOAKI (JP)
IMAI RYOZO (JP)
NAGIRA YOZO (JP)
MIKI RYUJI (JP)
TAOKA NAOAKI (JP)
IMAI RYOZO (JP)
Application Number:
PCT/JP2019/046068
Publication Date:
June 04, 2020
Filing Date:
November 26, 2019
Export Citation:
Assignee:
KANEKA CORP (JP)
NAT AGRICULTURE & FOOD RES ORG (JP)
NAT AGRICULTURE & FOOD RES ORG (JP)
International Classes:
A01K67/00; A01H5/00; C07K17/14; C12N5/00; C12N11/14; C12N15/09; C12N15/87; C12N9/16
Domestic Patent References:
| WO2017150733A1 | 2017-09-08 | |||
| WO2017195906A1 | 2017-11-16 |
Foreign References:
| JP2004532850A | 2004-10-28 | |||
| JP2001337065A | 2001-12-07 | |||
| US20180186842A1 | 2018-07-05 | |||
| JP2009516199A | 2009-04-16 | |||
| CN108498460A | 2018-09-07 |
Other References:
THOBHANI, S. ET AL.: "Bioconjugation and characterisation of gold colloid-labelled proteins", J. IMMUNOL. METHODS, vol. 356, no. 1-2, 2010, pages 60 - 69, XP027015644
MIZUTA, YOKO ET AL.: "Genome editing of pollen", RESEARCH PRESENTATION RECORD OF THE 82ND ANNUAL MEETING OF THE BOTANICAL SOCIETY OF JAPAN, vol. 82, September 2018 (2018-09-01), pages 256
MIZUTA, YOKO ET AL.: "Genome editing of pollen", RESEARCH PRESENTATION RECORD OF THE 82ND ANNUAL MEETING OF THE BOTANICAL SOCIETY OF JAPAN, vol. 82, September 2018 (2018-09-01), pages 256
Attorney, Agent or Firm:
HIROTA, Koichi et al. (JP)
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