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Patent Searching and Data


Title:
GENOME-MODIFIED MICROBIAL STRAIN
Document Type and Number:
WIPO Patent Application WO/2022/239871
Kind Code:
A1
Abstract:
The present disclosure relates to the following: a genetically modified microbial strain that metabolizes a sugar to alcohol, into which mutation has been introduced into two or more genes by a serial breeding procedure; the use of this genetically modified microbial strain; and a method for producing a genetically modified microorganism, comprising a step of introducing mutation, in a microorganism that metabolizes a sugar to alcohol, into at least two genes by a serial breeding procedure.

Inventors:
OHYA YOSHIKAZU (JP)
CHADANI TOMOYA (JP)
AKAO TAKESHI (JP)
GOSHIMA TETSUYA (JP)
Application Number:
PCT/JP2022/020282
Publication Date:
November 17, 2022
Filing Date:
May 13, 2022
Export Citation:
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Assignee:
UNIV TOKYO (JP)
NATIONAL RES INSTITUTE OF BREWING (JP)
International Classes:
C12N15/09; C12G3/022; C12N1/19; C12N15/10; C12N15/31; C12N15/55
Foreign References:
JP2013169191A2013-09-02
JP2018011553A2018-01-25
Other References:
MITSUI RYOSUKE; YAMADA RYOSUKE; OGINO HIROYASU: "CRISPR system in the yeastSaccharomyces cerevisiaeand its application in the bioproduction of useful chemicals", WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER NETHERLANDS, DORDRECHT, vol. 35, no. 7, 6 July 2019 (2019-07-06), Dordrecht, pages 1 - 9, XP036828213, ISSN: 0959-3993, DOI: 10.1007/s11274-019-2688-8
VAN WYK NIËL, KROUKAMP HEINRICH, ESPINOSA MONICA I., VON WALLBRUNN CHRISTIAN, WENDLAND JÜRGEN, PRETORIUS ISAK S.: "Blending wine yeast phenotypes with the aid of CRISPR DNA editing technologies", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, ELSEVIER BV, NL, vol. 324, 1 July 2020 (2020-07-01), NL , pages 108615, XP093004020, ISSN: 0168-1605, DOI: 10.1016/j.ijfoodmicro.2020.108615
OHNUKI SHINSUKE, KASHIMA MAO, YAMADA TOSHIKAZU, GHANEGOLMOHAMMADI FARZAN, ZHOU YAN, GOSHIMA TETSUYA, MARUYAMA JUN-ICHI, KITAMOTO K: "Genome editing to generate nonfoam-forming sake yeast strains", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, JP, vol. 83, no. 8, 3 August 2019 (2019-08-03), JP , pages 1583 - 1593, XP093004019, ISSN: 0916-8451, DOI: 10.1080/09168451.2019.1631146
CHIN YOUNG-WOOK; KANG WOO-KYUNG; JANG HAE WON; TURNER TIMOTHY L.; KIM HYO JIN: "CAR1deletion by CRISPR/Cas9 reduces formation of ethyl carbamate from ethanol fermentation bySaccharomyces cerevisiae", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 43, no. 11, 29 August 2016 (2016-08-29), GB , pages 1517 - 1525, XP036078060, ISSN: 1367-5435, DOI: 10.1007/s10295-016-1831-x
MAKIMOTO JUN; WAKABAYASHI KOU; INOUE TOYOHISA; IKEDA YURIKO; KANDA RYOKO; ISOGAI ATSUKO; FUJII TSUTOMU; NAKAE TAKASHI: "Mutagenesis, breeding, and characterization of sake yeast strains with low production of dimethyl trisulfide precursor", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 130, no. 6, 13 August 2020 (2020-08-13), NL , pages 610 - 615, XP086350766, ISSN: 1389-1723, DOI: 10.1016/j.jbiosc.2020.07.011
TRINDADE DE CARVALHO ET AL: "Identification of novel alleles conferring superior production of rose flavor phenylethyl acetate using polygenic analysis in yeast", MBIO, vol. 8, 7 November 2017 (2017-11-07), pages 1 - 21, XP002786449
CHADANI TOMOYA, OHNUKI SHINSUKE, ISOGAI ATSUKO, GOSHIMA TETSUYA, KASHIMA MAO, GHANEGOLMOHAMMADI FARZAN, NISHI TOMOYUKI, HIRATA DAI: "Genome Editing to Generate Sake Yeast Strains with Eight Mutations That Confer Excellent Brewing Characteristics", CELLS, vol. 10, no. 6, 24 May 2021 (2021-05-24), pages 1299, XP093004016, DOI: 10.3390/cells10061299
CHADANI TOMOYA: "Breeding using genome editing of sake yeast with multiple excellent brewing characteristics", SUMMARY OF MASTER'S THESIS. THE UNIVERSITY OF TOKYO, 24 March 2022 (2022-03-24), XP093004015, Retrieved from the Internet [retrieved on 20221201]
Attorney, Agent or Firm:
ONO Makoto et al. (JP)
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