Title:
GLUCOSE DEHYDROGENASE
Document Type and Number:
WIPO Patent Application WO/2013/051704
Kind Code:
A1
Abstract:
Provided are a glucose dehydrogenase originating in Botryotinia fuckeliana, a method for producing the same, and an assay method, a reagent composition and a biosensor each using the aforesaid enzyme.
A biosensor for assaying glucose without being affected by dissolved oxygen, characterized by using a flavin-bound glucose dehydrogenase that comprises glycopolypeptide (e) or (f):
(e) a glycopolypeptide comprising an amino acid sequence represented by SEQ ID NO:5; and
(f) a glycopolypeptide comprising an amino acid sequence that is derived from the amino acid sequence represented by SEQ ID NO:5 by substitution, deletion or addition of one to several amino acids and having glucose dehydrogenase activity.
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Inventors:
HONDA MICHINARI (JP)
TAKI SAYAKA (JP)
TAKENAKA RYO (JP)
TAKI SAYAKA (JP)
TAKENAKA RYO (JP)
Application Number:
PCT/JP2012/075999
Publication Date:
April 11, 2013
Filing Date:
October 05, 2012
Export Citation:
Assignee:
IKEDA FOOD RES CO LTD (JP)
International Classes:
C12N15/09; C12M1/40; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/32; G01N33/66
Domestic Patent References:
| WO2012001976A1 | 2012-01-05 |
Foreign References:
| JP2006502810A | 2006-01-26 | |||
| JP2004264121A | 2004-09-24 |
Other References:
DATABASE DDBJ/EMBL/GENBANK 5 January 2004 (2004-01-05), ROLKE Y. ET AL.: "Definition: Botryotinia fuckeliana god1 gene for glucose oxidase, specimen voucher SAS 56.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/aj555871 accession no. J555871
SUNG YUN-MO ET AL.: "Immobilization of Glucose Oxidase Using Pyrrole-Titania Nanohybrids for Electrochemical Biosensor Applications", ELECTROCHEM SOLID-STATE LETT, vol. 8, no. 2, 2005, pages H24 - H26
YVONNE ROLKE ET AL.: "Functional analysis of H202-generating systems in Botrytis cinerea: the major Cu-Zn-superoxide dismutase (BCSOD1) contributes to virulence on French bean, whereas a glucose oxidase (BCGOD1) is dispensable", MOLECULAR PLANT PATHOLOGY, vol. 5, no. 1, 2004, pages 17 - 27, XP055084402, DOI: doi:10.1111/j.1364-3703.2004.00201.x
SUNG YUN-MO ET AL.: "Immobilization of Glucose Oxidase Using Pyrrole-Titania Nanohybrids for Electrochemical Biosensor Applications", ELECTROCHEM SOLID-STATE LETT, vol. 8, no. 2, 2005, pages H24 - H26
YVONNE ROLKE ET AL.: "Functional analysis of H202-generating systems in Botrytis cinerea: the major Cu-Zn-superoxide dismutase (BCSOD1) contributes to virulence on French bean, whereas a glucose oxidase (BCGOD1) is dispensable", MOLECULAR PLANT PATHOLOGY, vol. 5, no. 1, 2004, pages 17 - 27, XP055084402, DOI: doi:10.1111/j.1364-3703.2004.00201.x
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
Patent business corporation Alga patent firm (JP)
Patent business corporation Alga patent firm (JP)
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