Title:
GLYCYRRHETINIC ACID-PRODUCING RECOMBINANT SACCHAROMYCES CEREVISIAE, CONSTRUCTION METHOD FOR SAME, AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/120983
Kind Code:
A1
Abstract:
Provided are a Glycyrrhetinic acid-producing recombinant yeast strain, specifically the strain of deposit number CGMCC 13126, a preparation method for the strain, and uses of the strain in producing Glycyrrhetinic acid. The strain overexpresses yeast genes ERG10, ERG12, ERG13, ERG19, ERG20, ERG9, ERG1, ERG8, IDI1, and tHMG, expresses β-AS, CYP88D6, and CYP72A154 genes in a Glycyrrhetinic acid synthesis pathway and a cytochrome P450 reductase gene of Arabidopsis thaliana, and deletes BTS1 at the same time.
Inventors:
CHEN SHILIN (CN)
WANG CAIXIA (CN)
SUN WEI (CN)
SU XINYAO (CN)
ZHANG MENGTING (CN)
SUN MENGCHU (CN)
WANG CAIXIA (CN)
SUN WEI (CN)
SU XINYAO (CN)
ZHANG MENGTING (CN)
SUN MENGCHU (CN)
Application Number:
PCT/CN2017/105678
Publication Date:
July 05, 2018
Filing Date:
October 11, 2017
Export Citation:
Assignee:
INST OF CHINESE MATERIA MEDICA CHINA ACADEMY OF CHINESE MEDICAL SCIENCE (CN)
International Classes:
C12N1/19; C12N15/81; C12P33/00; C12R1/865
Domestic Patent References:
| WO2012106405A1 | 2012-08-09 |
Foreign References:
| CN106635853A | 2017-05-10 |
Other References:
31 October 2012 (2012-10-31)
31 May 2014 (2014-05-31)
MOURBOUL ABLISE: "Synthesis and in Vitro Antioxidant Activity of Glycyrr- hetinic Acid Derivatives Tested with the Cytochrome P450/NADPH System", CHEMICAL & PHARMACEUTICAL BULLETIN, vol. 52, no. 12, 31 December 2004 (2004-12-31), pages 1436 - 1439, XP055294678, ISSN: 0009-2363, Retrieved from the Internet
31 May 2014 (2014-05-31)
MOURBOUL ABLISE: "Synthesis and in Vitro Antioxidant Activity of Glycyrr- hetinic Acid Derivatives Tested with the Cytochrome P450/NADPH System", CHEMICAL & PHARMACEUTICAL BULLETIN, vol. 52, no. 12, 31 December 2004 (2004-12-31), pages 1436 - 1439, XP055294678, ISSN: 0009-2363, Retrieved from the Internet
Attorney, Agent or Firm:
GE CHENG & CO., LTD. (CN)
Download PDF:
Previous Patent: DOUBLE-LAYERED DRAINAGE TUBE
Next Patent: METHOD AND TERMINAL FOR PERFORMING SYNCHRONIZATION
Next Patent: METHOD AND TERMINAL FOR PERFORMING SYNCHRONIZATION