Melatonin, N-acetyl-5-methoxytryptamine, is a hormone from the pineal gland, isolated by Lerner & al. (J. Am. chem. Soc., 80,1958,2587), which has formed the subject of numerous studies for its circadian activity, in the rhythm of sleep, for its effects on the production of testosterone, for its activity on the hypothalamus and in psychiatric disorders.

It has thus been envisaged to employ melatonin and analogues thereof especially for the treatment of depression and psychiatric disorders, in particular stress, anxiety, depression, insomnia, schizophrenia, psychoses and epilepsy, and also for the treatment of sleeping disorders associated with travelling ("jet lag"), neurodegenerative diseases of the central nervous system such as Parkinson's disease or Alzheimer's disease, for the treatment of cancers or, alternatively, as a contraceptive or as an analgesic.

However, the direct use of melatonin in vivo has not proved to be very satisfactory, on account of the facts that <BR> <BR> <BR> the first passage through the liver extracts more than 90 % of the active principle and that hypnotic activity of melatonin has not been clearly demonstrated.

Various melatonin analogues have been described, demonstrating two research routes which relate either to melatonin substituents (WO-A-89/01472, US-A-5 283 343, US-A- 5 093 352 and WO-A-93/11761) or to the aromatic ring by replacing the indolyl group by a naphthyl group (FR-A-2 658 818, FR-A-2 689 124).

We have demonstrated that melatonin does not exhibit any hypnotic activity; but is the bioprecursor of acetylated metabolites which induce sleep.

The present patent application proposes a novel route for the development of novel carboline derivatives which are analogues of endogenous acetylated metabolites of melatonin.

The present invention relates to novel carboline derivatives of general formula I (I'and I'') :

In which X represents a divalent radical of formula R1, R2, R3, and R4 represent, independently of each other, a hydrogen atom, a hydroxy radical, a lower alkyl, lower cycloalkyl, lower alkoxy, aryloxy, lower aralkyloxy, halo or nitro radical or an unsaturated aliphatic chain, formyl, lower alkylcarbonyl, lower alkylcarbonyloxy, halo (lower) alkylcarbonyl, halo (lower) alkylcarbonyloxy, halo- (lower) alkyl, halo (lower) alkyloxy, lower alkoxycarbonyl, carboxyl, optionally substituted carboxamide, two adjacent

radicals Rl, R2, R3 or R4 being able to form together a 2,3- dihydropyranyl group which is possibly bearing an oxo group, Rl, R2, R3, and R also represent, independently of each <BR> <BR> <BR> <BR> <BR> other, a perhalo (lower) alkyl, aryl, aralkyl, lower cycloalkoxy, polyhaloalkoxy, a thiol radical, lower alkylthio, lower cycloalkylthio, mono or polyhaloalkylthio, arylthio, aralkylthio, formate, a lower cycloalkyl- carbonyloxy, arylcarbonyloxy, aralkylcarbonyloxy, lower dialkylaminoalkyl, aminoalkyl, lower alkylaminoalkyl, arylaminoalkyl, diarylaminoalkyl, arylalkylaminoalkyl, amino, alkylamino, dialkylamino, arylamino, diarylamino, aralkylamino, diaralkylamino, formamide, a lower alkyl- carbonylamino, a lower cycloalkylcarbonylamino, halo (lower)- alkylcarbonylamino, polyhalo (lower) alkylcarbonylamino, aryl- carbonylamino, lower aralkylcarbonylamino, a lower cyclo- alkylcarbonyl, polyhalo (lower) alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, lower cycloalkoxycarbonyl, haloalkoxy- carbonyl, aryloxycarbonyl, aralkyloxycarbonyl, a lower alkylsulfonyl, a lower cycloalkylsulfonyl, halo (lower) alkyl- sulfonyl, perhalo (lower) alkylsulfonyl, arylsulfonyl, aralkylsulfonyl, cyano radical.

Rs represents a hydrogen atom, a lower alkyl, cycloalkyl, aryl, lower aralkyl, lower alkoxy, a lower alkylcarbonyl, halo (lower) alkylcarbonyl, lower alkyloxy- carbonyl, amino, alkylamino, dialkylamino, alkylarylamino, diarylamino, halo (lower) alkylsulfonyl, alkylsulfonyl or arylsulfonyl radical, R5 also represents a halo (lower) alkyl, perhalo (lower) alkyl, a hydroxy radical, lower cycloalkoxy, aryloxy, aralkyloxy, a thiol radical, lower alkylthio, lower cycloalkylthio, arylthio, aralkylthio, dialkylaminoalkyl, arylamino, aralkylamino, diaralkylamino, optionally

substitued carboxamide, formamide, formyl, a lower cycloalkylcarbonyl, perhalo (lower) alkylcarbonyl, aryl- carbonyl, aralkylcarbonyl, lower cycloalkoxycarbonyl, haloalkoxycarbonyl, aryloxy-carbonyl, aralkyloxycarbonyl, a lower cycloalkylsulfonyl, perhalo (lower) alkylsulfonyl, aralkylsulfonyl, cyano radical.

R6 R7 R9 Rlo, Rl2, R13, Rla and R15 represent, independently of each other, a hydrogen atom, a lower alkyl, a lower cycloalkyl, halo (lower) alkyl, perhalo (lower) alkyl, aryl, aralkyl, lower alkoxy, lower cycloalkoxy, mono or polyhaloalkoxy, aryloxy, aralkyloxy, hydroxyalkyl, alkyloxy- alkyl, lower alkylthio, lower cycloalkylthio, mono or polyhaloalkylthio, arylthio, aralkylthio, formate, lower alkylcarbonyloxy, a lower cycloalkylcarbonyloxy, halo- (lower) alkylcarbonyloxy, arylcarbonyloxy, aralkyl- carbonyloxy, dialkylaminoalkyl, dialkylamino, diarylamino, diaralkylamino, optionally substitued carboxamide, formamide, a lower alkylcarbonylamino, a lower cyclo- alkylcarbonylamino, halo (lower) alkylcarbonylamino, perhalo- (lower) alkylcarbonylamino, lower arylcarbonylamino, lower aralkylcarbonylamino, formyl, a lower alkylcarbonyl, a lower cycloalkylcarbonyl, halo (lower) alkylcarbonyl, perhalo- (lower) alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, carboxyl, lower alkoxycarbonyl, lower cycloalkoxycarbonyl, haloalkoxycarbonyl, aryloxycarbonyl, aralkyloxycarbonyl, a lower alkylsulfonyl, a lower cycloalkylsulfonyl, halo- (lower) alkylsulfonyl, perhalo (lower) alkylsulfonyl, aryl- sulfonyl, aralkylsulfonyl, halo, cyano or nitro radical.

R8 represents a lower alkyl, a lower cycloalkyl, hydroxyalkyl, alkyloxyalkyl, halo (lower) alkyl, perhalo- (lower) alkyl, aryl, lower-alkyl aryl, halo aryl, lower- alkoxy aryl, nitro aryl, amino aryl, di (lower) alkylamino

aryl, pyridyl, aralkyl, lower alkoxy, lower cycloalkoxy, mono or polyhaloalkoxy, aryloxy, aralkyloxy, lower alkylthio, lower cycloalkylthio, mono or polyhaloalkylthio, arylthio, aralkylthio, lower alkylcarbonyloxy, a lower cyclo-alkylcarbonyloxy, halo (lower) alkylcarbonyloxy, aryl- <BR> <BR> <BR> carbonyloxy, aralkylcarbonyloxy, dialkylaminpalkyl, dialkylamino, diarylamino, diaralkylamino, optionally substitued carboxamide, formamide, a lower alkylcarbonyl- amino, a lower cycloalkylcarbonylamino, halo (lower)- alkylcarbonylamino, polyhalo (lower) alkylcarbonylamino, aryl- carbonylamino, lower aralkyl-carbonylamino, formyl, a lower alkylcarbonyl, a lower cyclo-alkylcarbonyl, halo (lower)- alkylcarbonyl, perhalo (lower) alkylcarbonyl, aryl-carbonyl, aralkylcarbonyl, carboxyl, lower alkoxycarbonyl, lower cycloalkoxycarbonyl, haloalkoxycarbonyl, aryloxy-carbonyl, aralkyloxycarbonyl, a lower alkylsulfonyl, a lower cycloalkylsulfonyl, halo (lower) alkylsulfonyl, perhalo- (lower) alkylsulfonyl, arylsulfonyl, aralkylsulfonyl, halo, cyano or nitro radical.

R6, R7, Rg, Rg, Rlo, R12, R13 and R15 can represent, but not simultaneously R8 and R15, Rg and Rlo, R12 and R13 a hydroxyl radical, a thiol radical, amino, alkylamino, arylamino, aralkylamino, alkoxy, lower cycloalkoxy, mono or polyhaloalkoxy, aryloxy, aralkyloxy, lower alkylthio, lower cycloalkylthio, mono or polyhalogenoalkylthio, arylthio, aralkylthio, alkylamino, arylamino.

Furthermore can also represent a carbonyl group C=O, a thiocarbonyl group C=S, a radical C=N-R16, or a radical

Rll represents an oxygen atom, a sulfur, R16 represents a hydrogen atom, a lower alkyl, a lower cycloalkyl, aryl, aralkyl, a hydroxy radical, lower alkoxy, alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, amino, alkyl- amino, dialkylamino, arylamino, diarylamino, aralkylamino, alkylcarbonylamino, arylcarbonylamino.

R17 and R18 represent independtly of each other a hydrogen atom, a lower alkyl, a lower cycloalkyl, aryl, aralkyl, lower alkoxy, lower cycloalkoxy, aryloxy, aralkyloxy, lower alkylthio, arylthio, aralkyltio, lower alkylcarbonyloxy, lower cycloalkylcarbonyloxy, aryl- carbonyloxy, aralkyl-carbonyloxy, dialkylamino, aryl- alkylamino, diarylamino, optionnaly substitued carboxamide, formamide, lower alkylcarbonylamino, lower cyclo- alkylcarbonylamino, aryl-carbonylamino, formyl, lower alkylcarbonyl, lower cycloalkylcarbonyl, arylcarbonyl,

aralkylcarbonyl, carboxyl, lower alkoxycarbonyl, aryloxycarbonyl, aralkyloxycarbonyl, lower alkylsulfonyl, arylsulfonyl.

It is possible for Rl-It2, Ft2-R3, elrld R3-Rq part of another aromatic or non-aromatic ring with or without a hetero atom and optionally bearing a carbonyl or thiocarbonyl group.

Compounds, according to the present invention, may contain one to three assymetric centers, such compounds will exist as optical isomers (enantiomers).

The present invention concerns the racemic mixtures thereof, the pure enantiomers thereof or the mixtures thereof in all proportions, and the therapeutically acceptable salts thereof.

More particularly, the carbon atom bearing the R8 group may be an assymetric center, the present invention includes all such enantiomers and mixtures.

The expressions lower alkyl, lower alkoxy or perhalo (lower) alkyl are generally understood to refer to radicals whose alkyl residue comprises between 1 and 6 carbon atoms.

These are preferably linear or branched C1-C4 alkyl residues chosen more particularly from methyl, ethyl, n- propyl, i-propyl, n-butyl, i-butyl and t-butyl groups. The expression unsaturated aliphatic chain is preferably understood to refer to an unsaturated C2-C6 hydrocarbon chain.

The term aryl generally denotes aromatic and heteroaromatic groups, in particular aryls chosen from phenyl, thienyl, furanyl, pyridyl and naphthyl groups. The aryl radicals may also be substituted with one or more groups chosen in particular from a hydrogen atom, a lower alkyl, a lower cycloalkyl, halo (lower) alkyl, perhalo-

(lower) alkyl, aryl, aralkyl, a hydroxy radical, lower alkoxy, lower cycloalkoxy, mono or polyhaloalkoxy, aryloxy, aralkyloxy, a thiol radical, lower alkylthio, lower cycloalkylthio, mono or polyhaloalkylthio, arylthio, aralkylthio, formate, lower alkylcarbonyloxy, a lower cycloalkylcarbonyloxy, halo (lower) alkylcarbonyloxy, aryl- carbonyloxy, aralkylcarbonyloxy, dialkylaminoalkyl, amino, alkylamino, dialkylamino, arylamino, diarylamino, aralkyl- amino, diaralkylamino, optionally substitued carboxamide, formamide, a lower alkylcarbonylamino, a lower cycloalkyl- carbonylamino, halo (lower) alkylcarbonylamino, perhalo- (lower) alkylcarbonylamino, lower arylcarbonylamino, lower aralkylcarbonylamino, formyl, a lower alkylcarbonyl, a lower cycloalkylcarbonyl, halo (lower) alkylcarbonyl, perhalo- (lower) alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, carboxyl, lower alkoxycarbonyl, lower cycloalkoxycarbonyl, haloalkoxycarbonyl, aryloxycarbonyl, aralkyloxycarbonyl, a lower alkylsulfonyl, a lower cycloalkylsulfonyl, halo- (lower) alkylsulfonyl, perhalo (lower) alkylsulfonyl, aryl- sulfonyl, aralkylsulfonyl, halo, cyano or nitro radicals.

The expression lower aralkyl will be understood to refer to the combination of a lower alkyl and an aryl as defined above. This will preferably be the benzyl radical, which is optionally substituted.

The halo radicals are preferably chosen from fluorine, chlorine, bromine and iodine atoms.

The perhalo radicals are preferably perfluoro radicals.

When R1-R2 R2-R3 and R3-R4 form part of another aromatic ring, with or without a hetero atom, this is preferably another benzene ring, which is optionally substituted, or a pyridyl ring, which is optionally substituted.

When R1-R2 R2-R3 and R3-R4 form part of another non- aromatic ring, they preferably form together a divalent radical of formula-0- (CH2) m-, m being equal to 2 or 3, <BR> <BR> <BR> <BR> <BR> which is optionally substituted, or a divalent radical of formula -O-(CH2)p-O-, p being equal to 1 or 2, which is optionally substituted. When the derivatives comprise at least one asymmetric carbon, the present invention relates to the corresponding racemic mixtures, as well as to the pure enantiomers thereof or the mixtures thereof in all proportions.

The therapeutically acceptable salts of the derivatives according to the invention are the usual organic or inorganic salts of the art, in particular the hydrochlorides, the tosylates, the mesylates and the citrates, as well as solvates such as the hydrates or hemihydrates of the compounds of general formula I.

By comparison with the derivatives described in WO 96/08490, the derivatives according to the invention are characterised in that they contain a R8 group which is different from an hydrogen atom, which increases dramatically the stability of such compounds in gastric acidic medium and allows oral administration to be used.

Without being bind to any theory, it has now been found that the group responsible of the hypnotic activity of the compounds according to the present invention is the ene- amide or dihydroene-amide group. This ene-amide or dihydroene-amide group is the group constituted by the Ca-N- Cb in formula I.

Derivatives according to the present invention and being of particular interest are these wherein : Ru represents preferably an oxygen atom or sulfur atom.

R2 represents a hydroxy or lower alkoxy radical, preferably R2 represents a methoxy radical. represents a hydrogen atom or a lower alkyloxy- carbonyl group.

The present invention relates more particularly to the derivatives of general formula la (Ia'and Ia'').: When Ia represents Ia', R8 represents an alkyl radical, optionally substitued by alkyl, halide, amino, alkyloxy groups, alkyloxycarbonyl, an aryl radical optionally substitued by alkyl, halide, nitro, dialkylamino, alkyloxy group, alkyloxycarbonyl, alkylamino, this aryl radical being e. g. a phenyl group, a pyridyl group When Ia represents Ira", If R15 is an hydrogen atom, R8 represents preferably an ethyl, hexyl, isopropyl radical, a phenyl, fluorophenyl, methoxyphenyl, aminophenyl, dimethylaminophenyl, nitro- phenyl, tolyl radical, an ethoxycarbonyl radical, a pyridyl radical.

If R15 is different of an hydrogen atom, R8 and Ri.,, identical, represent lower alkyl radicals or aryl radicals

optionally substitued by alkyl, halide, amino, alkyloxy group.

X represents preferably a divalent radical of formula (R5 = R6R = H) Rll represents preferably an oxygen atom or sulfur atom.

R2 represents a hydroxyl or lower alkoxy radical and preferably a methoxy radical.

R3 represents an hydrogen atom or an alkyl radical and preferably a methyl radical.

The present invention also relates to the process for the preparation of the derivatives of general formula I as defined above.

In the particular of derivatives of general formula Ib.

For which R2, R3, R8 R14 and X are defined above.

The derivative of formula Ib may be obtained directly by reacting the compound of general formula IIa.

For which R2, R3, Rs, R14 and X are defined above, with a carboxylic acid (such as acrylic acid) in the presence or not of diphenylphosphorylazide or with acrylonitrile.

In order to obtain the derivatives of general formula IIa, a Bischler-Napieralski reaction is carried out by reacting the compounds of general formula IIIa.

In which R2, R3, R8, R14 and X are defined above, with phosphorus pentoxide (P205) or with phosphorus oxychloride (POC13) in a suitable solvent, for example toluene, xylene, dichloromethane.

These derivatives IIa may also be prepared by permanganic oxidation of the derivatives of general formula IIb.

Wherein R2, R3, R8 and R14 are defined above.

In order to obtain the derivatives of general formula IIb, a Pictet-Spengler reaction is carried out by reacting the derivatives of general formula IIIb.

For which R2, R3, Ri and X are defined above, with compounds of formula Rg-CH2-CHO or chemical equivalents thereof such as a ketal, an enol ether, an enol ester or a nitrile of formula Rg-CH2-CN under reducing conditions, R8 being defined above.

The derivatives of formula IIIa may be obtained by carrying out an acylation by an acylating agent (acid chloride, acid anhydride, ester) on the derivatives of <BR> <BR> <BR> <BR> <BR> formula IIIb for which R2, R3, R14 and X are defined above.<BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <P> Also, when X=NR5 the derivatives of formula IIIa may be obtained by Fischer reaction with the appropriate substituted phenylhydrazine of general formula IV and the suitable aldehyde or masked aldehyde such ketal of general formula V.

For which R2, R3, Rg, R14 are defined above R5 represents an hydrogen radical or a lower alkyl In the particular case of derivatives of general formula Ic For which R2, R3, Rg, Rl4 and X are defined above, the derivative of formula Ic may be obtained directly by catalytic reduction by hydrogen with Palladium on Carbon of compound of general formula Ib.

In the particular case of derivatives of general formula Id

For which R2 R3, R8 R14 and X are defined above, the derivatives of general formula Id may be obtained directly by reaction of the Lawesson's reagent or by P2S4 on the compounds of general formula Ib.

In the particular case of derivatives of general formula Ie for which R2, R3, Rg, R14 and X are defined above, the derivative of formula Ie may be obtained directly by reaction of the Lawesson's reagent or by P2S4 on the compound of general formula Ic.

In the particular case of derivatives of general formula If for which R2, R3, Rg, Rll, R14 and X are defined above, the derivative of formula If may be obtained directly with an oxidant (such 02 in alkaline medium) on the compound of general formula Ib or Id.

The derivatives according to formula Ia wherein Rg and R15 are identical may be obtained by carrying out a reaction on the derivatives of general formula IIIb with compounds of formula EtO-CO-C (Rg) (Rls)-coH or chemical equivalents thereof under reducing conditions The derivatives according to the invention may be used as medical product for the treatment of diseases associated with disorders of melatonin activity. The derivatives according to the invention present myorelaxing properties and they also may be use for the treatment of depression and psychiatric disorders, in particular stress, anxiety, depression, insomnia, schizophrenia, psychoses and epilepsy, and also for the treatment of sleeping disorders associated with travelling ("jet lag"), neurodegenerative diseases of the central nervous system such as Parkinson's disease or Alzheimer's disease, for the treatment of cancers or, alternatively, as a contraceptive or as an analgesic. They present hypnotic and sedative effects.

STABILITY IN ACIDIC MEDIUM AND PHARMACOLOGICAL ACTIVITY 1. STABILITY IN ACIDIC MEDIUM AND ORAL BIOAVAILABILITY IN BEAGLE DOG In order to study the influence of Hp group on the stability of cycle A when comparing compounds EXAMPLE A and EXAMPLE B (R8 = hydrogen atom) to compounds of Example 1, Example 4, Example 7, Example 8 and Example 11,30 pM of each of these compounds were dissolved into 5 ml of pH=l or pH=2 or pH=3 or pH=7 (reference) buffer. Solutions were stirred for 15 minutes at 37°C. Subsequent measurements of compounds amounts non hydrolyzed were measured using a high performance liquid chromatography method.

EXAMPLE A EXAMPLE B

Table I : Stability in acidic medium and oral bioavailability in beagle dogs Hydrolysis rate (%) Absolutebioavailability oral/lV in lBcaglc elogs pH=2pH=3pH=7(fastingconditions)ExamplepH=1 n° (refcrenc 1 < gastric 1) 1-1 < 2 e) A 100 100 100 0 <1% 14.58.70>35%179.5 13.05.40N.D.717.0 4 0 0 0 0 >20% 11 26. 0 27.0 30.0 0 N.D. B 95 95 90 0 <5% 8 0 0 0 0 100% N. D. : Not determined Results are presented in Table I as percentage of compounds hydrolyzed, as shown in Table I, when compared to compounds EXAMPLE A and EXAMPLE B which are totally hydrolyzed between pH=l and pH=3.

Compounds n°l, 4,7,8 and 11 are quite stable from pH=2, since less than 27 per cent of these compounds are hydrolysed at pH>2.

In conclusion, compounds wherein the Ro group (in general formula I) is different from a hydrogen atom do not undergo a total hydrolysis of cycle A. The stability in acidic gastric medium of compounds such as examples 1,4,8 allows their administration by oral route. Pharmacokinetic studies

of these compounds (Table I) have been carried out in beagle dogs; compounds exhibit absolute bioavailability of oral route relative to intravenous route respectively higher than 35% (compound 1), 20% (compound 4) and equal to 100* (compound 8), since EXAMPLE A and EXAMPLE B absoute bioavailability are lower than li. (EXAMPLE A) and 5% (EXAMPLE B).

Compounds n°l (see Table II), as well as compounds n°4 and 7 (see Table III) exhibit significant hypnotic effect in Beagle dogs when given orally.

2. HYPNOTIC ACTIVITY IN BEAGLE DOGS : EFFECTS ON WAKEFULNESS /SLEEP STATES IN BEAGLE DOGS Beagle dogs are kept in a metal cage, connected to 2 Schwartzer ED 24 Polygraph equipped with BRAINLAB software, through a flexible cable.

Test and control products are administered via the oral or intravenous routes by means of gastric tubes. Records are scored in 30 seconds epochs for active wake, drowsy, slow wave sleep (light sleep + deep sleep) and REM sleep. These criteria are according to SHELTON et al. (in SHELTON J., NISHINO S., VAUGHT J., DEMENT W. C. and MIGNOT E. Comparative effects of modafinil and amphetamine on daytime sleepiness and cataplexy of narcoleptic dogs. Sleep 19 (1); 29-38, 1996.), based upon frequency and amplitude patterns of EEG cortical tracing (fronto-frontal), EMG (upper neck muscles) and EOG (bilateral). Behaviour is also continuously monitored for each observation period of 120 minutes. Prior acclimatisation of the dogs take 5 days; and, to mimic similar stress caused by gastric tubing, a mixture water/PEG (50/50; V/V) was administered orally together with intravenous boluses.

WakE includes all episodes with low voltage mixed frequency tracing in which the EMG is not inhibited. During wakefulness dogs stand, sit, or lay down, and eyes are open.

Sleepiness : the drowsy state is scored when dogs lie quietly with eyes closed (50 p. cent or more per one epoch) and cortical EEG shows trains of relatively slow waves(4- 7 liz) without the development of sleep spindles. Synchronous waves at 4-7 Hz and 50-100 pV appear on a background of low voltage fast-wave activity.

EMG is moderately decreased compared to wake stage.

* Light deep sleep S slow wave sleep (SWS) (light sleep + deep sleep) : canines are relaxed with prone posture. EEG patterns are of higher amplitude than in the previous stage and sleep spindles (10-14 Hz) and/or K complexes must be present in light sleep, as EOG show slow eye movements or no movement. Deep sleep is scored when slow delta waves (< 4 Hz) constitute 20 p. cent of an epoch or more during deep sleep.

REM : rapid eye movement sleep is scored when dogs lay down, eyes closed with intermittent apparent fast muscle twitches. A low voltage mixed frequency EEG tracing is observed together with rapid eye movements and a drop in EMG activity.

Repartition and duration of wakefulness/sleep states in Beagle dogs have been measured after oral (and intravenous route for compoundof EXAMPLE 1) administrations of Placebo (vehicle = 10 ml of mixture ethanol/PEG 400/water; 10/

40/50; V/V/V) compounds of Example 1, Example 4 and Example 7.

Results are presented in Tables II and III as Durations and Latences of each stage.

Compounds of Example 1, Example 4 and Example 7 exhibit a potent hypnotic activity inducing a sleep characterized by a high proportion of slow wave sleep. Regarding duration and latencies of wake, drowsy and SWS stages, these 3 compounds induce a significant hypnotic effect.

TABLE II : Sleep stages and sleep latencies during a 120 minutes observation period<BR> Example 1 (test), and vehicule alone (Placebo : 10 ml of ethanol/PEG/water ; 10/40/<BR> administrations via the oral and the intravenous routes Mean (#S.D.) duration of stages in 8 dogs Mean (#S.D.) lat@ (minutes) administration Dose Route Wake Drowsy SWS REM Sleep Drowsy SWS (µmol/Kg) Placebo - oral 110.1 4.4 5.2 0.3 9.9 86.2 94.6 A B B C A (12.3) (5.8) (6.2) (0.9) (12.3) (34.9) (32.9) Compound 2.5 IV 77.4 14.9 23.9 3.8 42.6 33.0 49.4 of Example 1 C A A A C (15.9) (8.6) (12.5) (3.9) (15.9) (13.0) (18.1) 2.5 oral 81.1 12.2 23.6 3.3 39.1 49.1 56.3 C A A A B-C 1 (16.1) (5.3) (15.1) (3.4) (16.1) (25.7) (25.0) 5 oral 79.7 14.3 23.0 3.1 40.4 41.0 51.3 C A A A C (18.4) (4.6) (13.1) (3.7) (18.4) (15.4) (18.8) 10 oral 84.0 14.8 18.8 2.4 36.1 49.6 64.0 C A A A B-C @ (19.2) (9.9) (14.0) (3.5) (19.4) (24.1) (30.5) Anova Treatment p<0.001 p<0.01 p<0.001 not treated p<0.001 p<0.001 p<0.00@ Effect Student test - Newman - Keuls : means designed by the same letter (A, B or C) are not<BR> significantly different.

Table III : Sleep stages and sleep latencies during a 120 minutes observation perio@<BR> compound of example 4 (test), compound of example 7 (test) and vehicle alone (Placeb<BR> ethanol/PEG/water ; 10/40/50 ; V/V/V) administrations via the oral route Mean (#S.D.) duration of stages in 8 dogs Mean (#S.D.) la@ (minutes) administration Dose Route Wake Drowsy SWS REM Sleep Drowsy SWS (µmol/Kg) Placebo 0 oral 98.2 7.9 12.4 1.4 21.8 66.0 79.8 (13.9) (4.3) (7.5) (3.2) (13.9) (24.3) (22.2) Compound of 10 oral 73.8 10.4 31.5 4.4 46.3 50.4 58.1 Example 4 (21.3) (3.9) (17.6) (4.5) (21.3) (19.8) (27.0) Anova Treatment p<0.01 NS p<0.01 not treated p<0.01 NS p<0.0@ Effect Placebo 0 oral 98.2 7.9 12.4 1.4 21.8 66.0 79.8 (13.9) (4.3) (7.5) (3.2) (13.9) (24.3) (22.2) Compound of 5.0 oral 83.7 13.0 18.9 4.4 35.1 59.0 74.1 Example 7 (25.1) (8.6) (14.7) (6.1) (25.8) (20.0) (26.3) Anova Treatment p<0.01 NS p<0.01 not treated p<0.01 NS p<0.0 Effect

3. HYPNOTIC AND SEDATIVE EFFECTS IN CHICKS The hypnotic and sedative effects of the derivatives, according to the invention, prepared above (the test results of which are given in Table IV below) were compared with those of 3 reference products, diazepam, pentobarbital sodium and melatonin, as well as with 2 psychostimulant compounds with hallucinogenic properties : 10- methoxyharmalan and harmaline, which are 3y4-dihydro-ß- carbolines, in 10-to 14-day-old chicks of chair label JA657 strain. The animals are subjected to alternating programmes of lighting consisting of 12 h of darkness (20.00 h to 8.00 h) and 12 h of light (8.00 h to 20.00 h). The ambient temperature is 25°C during the first week of rearing of the chicks and 22°C from the second week onwards. During the day, the lighting is provided by a halogen lamp (300 W) placed 30 cm above the floor of the vivarium. During the tests, the live weights of the chicks ranged between 85 and 120 g. The tests are carried out between 14.00 and 15.00 h.

The chicks are allotted, in groups of 3, in identical 30 cm x 50 cm x 30 cm vivariums. The test products are administered intramuscularly (IM) into the pectoralis major muscle, as a solution in a 25/50/25 (V/V/V) ethanol/PEG 400/water mixture, at the rate of 0.2 ml of solution per 100 g of live weight. The doses administered for the test products (novel compounds of the invention and reference substances) ranged from 0.25 pmol to 2 pmol per 100 g of live weight. The placebo corresponds to 0.2 ml of the 25/50/25 (V/V/V) ethanol/PEG 400/water mixture.

The solutions of the tests products in the 25/50/25 (V/V/V) ethanol/PEG 400/water/mixture were prepared at the times of use by successive dilution of a stock solution, obtained from 2.5 to 20 umol of accurately weighed product,

to which were successively added 0.5 ml of pure ethanol and then 1 ml of PEG 400, agitated by ultrasound and then made up to 2 ml with 0.5 ml of distilled water for an injectable preparation. Table IV gives the results obtained after IM administration of doses of between 0.25 and 2 pmol of test products dissolved in 0.2 ml of the ethanol/PEG 400/ distilled water mixture (25/50/25; V/V/V), per 100 g of live weight. For each chick, the volume injecte is adjusted, as a function of the actual live weight, to 0.2 ml per 100 g of live weight.

The parameters observed are the locomotor activity and state of consciousness of the chicks for 2 h, i. e. the equivalent of 6 theoretical awake/asleep cycles for a chick of this age. They are recorded by video camera for 90 minutes, the first 30 minutes being the time for adaptation to the device.

The hypnotic and sedative effects of the test products on the diurnal activity of 10-to 14-day-old chicks subjected to a program of permanent lighting from birth for 48 h, and then to alternate lighting program of 12 h of daylight (8.00 h-20.00 h) and 12 h of darkness (20.00 h-8.00 h) up to the test date, are given in Table I below. The tests are carried out during the day between 14.00 h and 15.00 h.

For each test product, several series of measurements were taken on batches of 3 animals, each value indicated being the average in each batch of 3 chicks. When the number of batches is greater than or equal to 2, the figures indicated are the average limit values observed.

Table IV DoseFATSTSedationtimeCompoundDose (mg/kg)(min.)(min.)(min.)(µmol/100g) batches)NA010-35Placebo(24 1.16NA0NotdeterminedMelation0.5 1 (5 batches) 2. 32 NA 0 16-36 2 (5 batches) 4. 64NA047-) 05 (3batches)1.24NA0NotdeterminedPentobarbital0.5 1.336Notdetermined12.48 (4batches)1.423-610-50NotdeterminedDiazepam0.5 l (10 batches) 2.95 2-7 24-70 17-20 2 (3 batches) 5. 69 2-5 81-100 14-15 3NA0010-methoxyharmalan1.4 3NA00Harmaline1.4 0.250.74101615Example1 918170.501.48 1 (8 batches) 2. 96 6-14 28-101 5-20 0.752.3212231Example3 Example 4 0. 25 0. 8G t0 9 17 121180.501.72 t (4 batches) 3.44 13-15 13-27 22-32 0.752.44121010Example5 13.2481025Example6 0.51.5682810Example7 5353013.12 25.97151537Example8 13.07114316Example9 Example D. 0. 51. 809208 1 3. 60 8 25 24 13.62152923Example13 13.74101529Example14 13.826.51542Example16 13.8911738Example17 13.5851658Example18 13.4623533Example19

Ke Key : -NA: not applicable, the animals remained conscious throughout the period of observation; -FAT : falling-asleep time, equal to the time required to pass from the state of active consciousness to a non- conscious state; -ST : sleep time, equal to the duration of the period of sleep from falling asleep to waking up; -Sedation time : after waking up, period of inactivity corresponding to stage 2 defined above.

In the conditions under which the test is carried out (times of administration, in the phase during whi. ch the animals receive light, between 14.00 h and 15.00 h) melatonin has no hypnotic activity.

By successively subjecting chicks to programmes of alternats and permanent lighting, we have demonstrated experimentally that melatonin has no direct hypnotic activity which is intrinsic to its structure. Its hypnotic activity depends on the activity of the enzyme N-acetyltransferase (NAT) in the pineal gland of the chick at the time of administration of the melatonin. The NAT enzyme is an acetylation enzyme. In the presence of the NAT enzyme in the pineal gland of the chick, the IM administration of melatonin induces a hypnotic effect of strong intensity (sleep time of between 250 and 300 minutes for a dose equal to 1 pmol of melatonin/100 g of live weight). Melatonin is thus the precursor of acetylated metabolites with direct hypnotic activity. Compounds of the invention are analogues of the hypnotic acetyl metabolites of melatonin.

In contrast with melatonin, all of the derivatives of the invention described above have direct hypnotic and sedative activities, which are independent of the time of administration, i. e. of the level of N-acetyltransferase enzyme in the CNS.

The results obtained show, for the derivatives according to the invention, a hypnotic effect which is higher than that of the reference products (pentobarbital, melatonin) and equivalent or even superior to that of diazepam.

The derivatives according to the invention are thus particularly advantageous for the treatment of sleep disorders and diseases associated with disorders of melatonin activity.

EXAMPLES Example 1: ETCARB07 <BR> <BR> <BR> Formula : C18H20N2°2 M = 296,36 g. mol-1 Structure : 9-methoxy-l-ethyl-2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one Preparation: Paramethoxyphenylhydrazine sulfonate (5 g-20,7 mmol) and N (4,4-diithoxybutyl) butanamide (4,8 g-20,7 mmol) are mixed in commercial THF (85 ml) in a 500 ml flask. The medium is then heated to the THF reflux and acetic acid (25 t) is added dropwise (35 ml). The limpid yellow mixture is stirred for 6 h at a temperature comprised between 80 and 85°C. After cooling, the reacting medium is transferred in a 2 litre Erlenmeyer flask and it is basified by addition of a sodium carbonate saturated solution (-100 ml) pH >7. The organic phase is decanted, and the aqueous phase is twice extracted with ethyl acetate (2 X 100 ml). The organic phases are jointed and successively washed with a sodium carbonate saturated solution (70 ml) and with water (70 ml).

The obtained organic phase is dried over MgS04 and the solvent is evaporated under reduced pressure until crystals appear (-5 ml ethyl acetate). After dilution with diethylic ether (50 ml) this solution is left overnight in the refrigerator. The crystals are obtained by a filtration, washed with diethylic ether and dried under vacuum. Nl- (2- (5-methoxy-lH-3-indolyl) ethyl) butanamide (3,3 g-R= 61%) is then obtained.

A Bischler-Napieralski reaction on Nl- (2- (5-methoxy-lH-3- indolyl) ethyl) butanamide leads to the l-propyl-6-methoxy-3 4- dihydro-2-carboline.

Method 1: Acrylic acid (0.-11 ml, 1.1 cq.) is added to a solution of 1- propyl-6-methoxy-3,4-dihydro-2-carboline inDMF(20ml).g) Diphenylphosphoryl azide (2.1 ml, 1.06 eq.) dissolved in DMF (3 ml) is then added dropwise, followed by triethylamine (2.85 ml, 2.1 eq.). After recrystallization from ethyl acetate, 9-methoxy-1-ethyl-2,3,4,6,7,12-hexahydroindolo [2,3- a] quinolizin-4-one is recovered (1.6 g, 56%).

Method 2: Acrylic acid (1 eq.) dissolved in xylene is added to a solution of l-propyl-6-methoxy-3, 4-dihydro-2-carboline in xylene.

The reaction flask is equipped with a water separator and the medium is heated to reflux of the xylene for 24 h. The xylene is then distilled off under reduced pressure. The product is purified as above. <BR> <BR> <BR> <BR> <BR> <BR> <BR> <P>NMR: 1H (CDCl3) : 1.29 (t, 3H); 2.44 and 2. 54 (2m, 6H) ; 2.86 (t, 2H); 3.86 (s, 3H); 4.08 (t, 2H); 6.87 (dd 2.4 and 8.7 Hz, 1H); 6.95 (d 2.4 Hz, 1H); 7.27 (d 8.7 Hz, 1H); 8.04 (broad s, 1H) Mass spectrum: m/z: 296 (M+.), 281 (100) Exact mass: calculated 296.1524 found 296.1545 Melting point: 223°C

Example 2 : HECARB07 Formula : C22H2pN202 M = 352,47 g. mol-1 Structure : 9-methoxy-l-hexyl-2,3,4,6,7,12-hexahydroindolo [2,3-a]<BR> <BR> <BR> quinolizin-4-one Preparation : A Bischler-Napierals. ki reaction on Nl- (2- (5-methoxy-lH-3- <BR> <BR> <BR> indolyl) ethyl) octanamide leads to the 1-heptyl-6-methoxy-3, 4- dihydro-2-carboline.

Acrylic acid (1 eq.) dissolved in xylene is added to a <BR> <BR> <BR> solution of 1-heptyl-6-methoxy-3,4-dihydro-2-carboline in xylene.

The reaction flask is equipped with a water separator and the medium is heated to reflux of the xylene for 48 h. The xylene is then distilled off under reduced pressure. The product is recrystallized from ethyl acetate. <BR> <BR> <BR> <BR> <P>NMR: 1H (CDC13): 0.92 (t, 3H); 1.42 (m, 8H); 2.40 (t, 2H) ; 2.50 (m, 4H); 2.56 (t, 2H); 3.86 (s, 3H); 4.08 (t, 2H); 6.87 (dd 2.4 and 8.7 Hz, H); 6.94 (d 2.4 Hz, 1H); 7.23 (d 8.7 Hz, 1H); 8.04 (broad s, 1H) Mass spectrum: m/z: 352 (M+.), 281 (100) Melting point: 140°C

Example 3 : IPCARB07 : Formula : Cl9H22N202 M = 310,39 g. mol-1 Structure: 9-methoxy-l-isopropyl-2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one Preparation : A Bischler-Napieralski reaction on Nl- (2- (5-methoxy-lH-3- indolyl) ethyl)-3-methylbutanamide leads to the 1-isobutyl-6- methoxy-3, 4-dihydro-2-carboline.

Acrylic acid (1 eq.) dissolved in xylene is added to a solution of 1-isobutyl-6-methoxy-3,4-dihydro-2-carboline in xylene.

The reaction flask is equipped with a water separator and the medium is heated to reflux of the xylene for 48 h. The xylene is then distilled off under reduced pressure. The product is recrystallized from ethyl acetate.

NMR: 1H (CDCl3) : 1.20 (d, 6H); 2.35 and 2.47 (2m, 4H); 2.87 (t, 2H); 3.38 (m, 1H); 3.86 (s, 3H); 4.06 (t, 2H); 6.87 (dd 2.4 and 9 Hz, 1H); 6.95 (d 2.4 Hz, 1H); 7.32 (d 9 Hz, 1H); Mass spectrum: m/z: 310 (M+.), 295 (100) Melting point: 251-252°C Example 4 : PHCARB07 Formula : C22H20N202 M = 344,41 g. mol-1 Structure :

9-methoxy-l-phenyl-2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one Preparation : A Bischler-Napieralski reaction on Nl- (2- (5-methoxy-lH-3- indolyl) ethyl)-2-phenylacetamide leads to the 1-benzyl-6- methoxy-3,4-dihydro-2-carboline.

Acrylic acid (0.75 ml, 1.1 eq.) is added to a solution of 1- benzyl-6-methoxy-3,4-dihydro-2-carboline (2. 8 g) in DMF (20 ml).

Diphenylphosphoryl azide (2.1 ml, 1.06 eq.) dissolved in DMF (3 ml) is then added dropwise, followed by triethylamine (2.85 ml, 2.1 eq.). After separation on silica gel (chloroform/methanol eluent), 9-methoxy-1-phenyl-2,3,4,6,7, 12-hexahydroindolo [2, 3-1] quinolizin-4-one is recovered (1.6 g, 56 %).

NMR: 1H (CDCl3) : 2.71 (m, 4l {) ; 2.91 (t, 2H); 3.83 (s, 3H); 4.20 (t, 2H); 6.76 (dd 2.4 and 8.7 Hz, 1H); 6.84 (d 8.7 Hz, 1H); 6.90 (d 2.4 Hz, 1H); 6.93 (broad s, 1H); 7.42 and 7.50 (m, 5H) Mass spectrum: m/z: 344 (M+.) (100), 253 Melting point: 235°C Example 5 : C02ETCARB07 Formula: C19H20N2O4 M = 340,37 g. mol-1 Structure :

1-carbethoxy-9-methoxy-2,3,4,6,7,12-hexahydroindolo [2,3-a]<BR> <BR> <BR> <BR> <BR> <BR> quinolizin-4-one Preparation: A Bischler-Napieralski reaction on ethyl 3- ( (2- (5-methoxy-lH- 3-indolyl) ethyl) amino)-3-oxopropanoate leads to the correspon- dingcarboline.

Sodium hydroxide solution (1N, 3 ml) is added to a solution of the carboline obtained (800 mg) in benzene (10 ml), followed by tetrabutylammonium hydrogen sulphate (0.1 eq.).

Acryloyl chloride (0.27 ml) is then added at 0°C and the mixture is allowed to return to room temperature overnight.

The product is separated on silica gel (chloroform/methanol) and 1-carbethoxy-9-methoxy-2,3,4,6,7,12-hexahydroindiolo [2,3- a] quinolizin-4-one is thus obtained.

NMR: 1H (CDCl3): 1.42 (t, 3H); 2.60 (t, 2H); 2.85 (t, 2H); 2.97 (t, 2H); 3.89 (s, 3H); 4.30 (t, 2H); 4.35 (q, 2H); 6.96 (d 2.1 Hz, 1H); 7.01 (dd 2.1 and 7.5 Hz, 1H); 7.35 (d 7.5 Hz, 1H); Mass spectrum: m/z: 340 (M+#), 294 (100) Melting point: 174-175°C

Example 6: 6ETETCARB07 C20h24N2O2M=324,42g.mol-1Formula: Structure : 9-methoxy-1,10-diethyl-2,3,4,6,7,12-hexahydroindolo [2,3-a]<BR> <BR> <BR> <BR> <BR> <BR> quinolizin-4-one Preparation : A Bischler-Napieralski reaction on Nl- (2- (5-methoxy-6-ethyl- 1H-3-indolyl) ethyl) butanamide leads to the 7-ethyl-l-propyl-6- methoxy-3,4-dihydro-2-carboline.

Acrylic acid (0.22 ml, 1.1 eq.) is added to a solution of7- ethyl-l-propyl-6-methoxy-3, 4-dihydro-2-carboline (764 mg) in DMF (20 ml), acrylic acid (0.22 ml, 1.1 eq) is added.

Diphenylphosphoryl azide (1.06 eq.) dissolved in DMF (3 ml) is then added dropwise, followed by triethylamine (2.1 eq.).

After separation on silica gel (chloroform/methanol eluent) 9-methoxy-1,10-diethyl-2,3,4,6,7,12-hexahydroindolo[2,3-a] quinolizin-4-one is recovered (28%).

NMR: 1H (CDCl3) : 1.26 (m, 6H); 2.41 and 2.58 (2m, 6H); 2.70 (q, 2H); 2.87 (t, 2H); 3.87 (s, 3H); 4.07 (t, 2H); 6.88 (ls, 1H); 7.19 (s, 1H); 8.33 (broad s, 1H).

Mass spectrum: m/z: 324 (M+.), 309 (100) Melting point: 204°C

Example 7 : ETCARB07S Formula : C1gH20N20S M = 312,42 g. mol-1 Structure : 9-methoxy-l-ethyl-2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizine-4-thione Preparation: Lawesson's reagent (0.5 mmol) is added portionwise, at 110°C, to a solution of 9-methoxy-1-ethyl-2,3,4,6,7,12- hexahydroindolo [2,3-a] quinolizin-4-one (300 mg, 1.01 mmol) in anhydrous toluene (15 ml). After refluxing for 30 min. and evaporation of the toluene, the product is chromatographed on silica gel (99/1 chloroform/methanol eluent) and 9-methoxy-1-ethyl-2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizine-4-thione is thus recovered (60-yield).

NMR: 1H (CDCl3) : 1.32 (t, 3H); 2.32 (t, 2H), 2.65 (q, 2H); 2.98 (t, 2H); 3.08 (t, 2H); 3.89 (s, 3H); 4.80 (t, 2H); 6.91 (dd 2.4 and 8.7 Hz, 1H); 6.98 (d 2.4 Hz, 1H); 7.34 (d 8.7 Hz, 1H); 8.11 (broad s, 1H) Mass spectrum: m/z: 312 (M+.) (100), 297 Melting point: 118°C

Example 8 : ETDHCARB07 Formula : C18H22N2°2 M = 298,38 g. mol-1 Structure :

9-methoxy-1-ethyl-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizin-4-one Preparation: Sodium bicarbonate (500 mg) and palladium-on-charcoal are successively added to a solution of 9-methoxy-l-ethyl- 2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one (500 mg) in ethanol, and the mixture is stirred overnight under a hydrogen atmosphere. After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate. 9-Methoxy-l-ethyl-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizin-4-one is thus recovered (76.).

NMR: 1H (CDCl3) : 0.79 (t, 3H); 1.13 (2m, 2H); 1.95 (m, 2H); 2.20 (m, 1H); 2.45 (m, 2H); 2.77 (m, 2H); 3.87 (s, 3H); 4.88 (s, 1H); 5.09 (m, 1H); 6.78 (dd 2.4 and 8.7 Hz, 1H); 6.92 (d 2.4 Hz, 1H); 7.25 (d 8.7 Hz, 1H); 8.77 (broad s, 1H) Melting point: 207°C

Example 9 : ETNAPH7 <BR> <BR> Formula : C2pH21NO2 M = 307,39 g. mol-1 Structure : 11-ethyl-3-methoxy-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1- a] isoquinolin-8-one Preparation: POC13 (4.6 ml) is added to a refluxing solution of the N (2- (7- methoxy-napht-1-yl) ethyl) butanamide (2.27 g, 11.1 mmol) in toluene (80 ml). After 3 h, the toluene is removed under reduced pressure. The residue is taken up in KOH solution (40'). The mixture is then extracted with EtOAc (x 3). After drying the organic phase over MgSO4, the solvent is removed. The crude reaction product is dissolved in DMF (5 ml) and acrylic acid (0.94 ml, 1.2 eq.) is then added. Diphenylphosphoryl azide (2.7 ml, 1.1 eq.) dissolved in DMF (3 ml) is then added dropwise, followed by triethylamine (3.67 ml, 2.6 eq.). 11- ethyl-3-methoxy-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1- a] isoquinolin-8-one (329 mg) is obtained by recrystallization from a 20/80 EtOAc/P. E. mixture).

NMR: 1H (CDC13) : 1.17 (t, 3H); 2.38 (m, 4H); 2.58 (t, 2H); 3.16 (t, 2H); 3.82 (t, 2H); 3.95 (s, 3H); 7.17 (dd, 1H); 7.29 (d, 1H); 7.33 (d, 1H); 7.66 (d, 1H); 7.76 (d, 1H) Melting point: 105-107°C

Example 10 : PHNAPH7 Formula : C24H21NO2 M = 355,43 g. mol-1 Structure : 3-methoxy-11-phenyl-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1-a]isoquinolin-8-one Preparation : POC13 (1.4 ml) is added to a refluxing solution of the N (2-(7- methoxy-napht-1-yl) ethyl) phenylacetamide (600 mg) in toluene (100 ml). After 3 h, the toluene is removed under reduced pressure. The residue is taken up in KOH solution (40'b). The mixture is then extracted with EtOAc (x 3). After drying the organic phase over MgSO4, the solvent is removed. The crude reaction product is dissolved in DMF (4.5 ml) and acrylic acid (0.15 ml, 1.1 eq.) is then added. Diphenylphosphoryl azide (0.45 ml, 1.1 eq.) dissolved in DMF (1 ml) is then added dropwise, followed by triethylamine (0.55 ml, 2.1 eq.).

The 3-methoxy-11-phenyl-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2, 1-a] isoquinolin-8-one is recovered (170 mg, yield = 25S).

NMR: 1H (CDCl3) : 2.70 (m, 4H): 3.32 (t, 2H); 3.88 (t, 2H); 3.94 (s, 3H); 6.74 to 7.60 (10H) Melting point: 152-154°C

Example 11 : PHCARB07S Formula: C22H20NOS M = 360,47 g. mol-1 Structure : 9-methoxy-1-phenyl-2,3,4,6,7,12-hexahydroindolo [2,3-a]<BR> <BR> <BR> <BR> <BR> <BR> [beta] quinolizin 4-thione Preparation: Lawesson's reagent (180 mg, 0. 47 mmol) is added portionwise, at 110°C, to a solution of 9-methoxy-1-phenyl-2,3, 4, 6,7,12- hexahydroindolo [2,3-a] quinolizin-4-one (164 mg, 0.47 mmol) in anhydrous toluene (10 ml). After refluxing for 30 min. and evaporation of the toluene, the product is chromatographed on silica gel (chloroform eluent) and the 9-methoxy-1-phenyl- 7,12-hexahydroindolo [2,3-a] quinolizine-4-thione is thus recovered (130 mg, 76% yield).

NMR: 1H (CDCl3) : 1.32 (t, 3H); 2.32 (t, 2H), 2.65 (q, 2H); 2.98 (t, 2H) ; 3.08 (t, 2H); 3.89 (s, 3H); 4.80 (t, 2H); 6.91 (dd 2.4 and 8.7 Hz, 1H) ; 6.98 (d 2.4 Hz, 1H); 7.34 (d 8.7 Hz, 1H); 8.11 (broad s, 1H) Melting point: 180°C

Example 12 : DEETCARB07S Formula: C18H1gN20S M = 310,41g. mol-I Structure : 9-methoxy-1-ethyl-2,3,4,12-tetrahydroindolo [2,3-a]<BR> <BR> <BR> quinolizin-4-thione Preparation : Potassium tert-butoxide (665 mg, 5.9 mmol) is added to a solution of 9-methoxy-1-ethyl-2,3,4,6,7,12-hexahydroindolo (2,3-a] quinolizine-4-thione (500 mg, 1.6 mmol) in DMF (42 ml). After placing the reaction assembly under vacuum, the mixture is stirred under normal oxygen pressure overnight. Water (15 ml) and concentrated hydrochloric acid (3 ml) are then successively added. The solution is stored in a refrigerator for 4 hours. After filtration, 9-methoxy- 1-ethyl-2,3,4,12-tetrahydroindolo [2,3-a] quinolizine-4- thione is obtained (150 mg, Y = 30%).

NMR : NMR: 1H (CDCl3) : 1.38 (t, 3H), 2.93 (q, 2H), 3.06 (t, 2H), 3.90 (s, 3H), 5.06 (t, 2H), 7.03 (d+s, 2H), 7.19 (d, 1H), 7.38 (d, 1H), 7.62 (d, 1H), 8.43 (broad s, 1H) Mass spectrum: m/z: 310 (M+.), 295,155 Melting point: 212°C Example 13 : FPHCARB07 Formula : C22H19N2°2F M = 362,40g. mol' Structure:

9-methoxy-l- (p-fluorophenyl)-2,3,4,6,7,12-hexahydroindolo<BR> <BR> <BR> <BR> <BR> <BR> [2,3-a] quinolizin-4-one Preparation: A Bischler-Napieralski reaction, carried out on N- [2'- (5- methoxy-lH-3-indolyl)ethyl]-2- (p-fluorophenyl) acetamide (1.15 g, 3.5 mmol), results in the 1- (parafluorobenzyl)-6-methoxy- 3,4-dihydro-2-carboline (1.06 g), which is dissolved directly in anhydrous dimethylformamide (DMF) (10 ml).

This mixture is cooled to 0°C and acrylic acid (0.24 ml, 3.5 mmol), diphenylphosphoryl azide ((PhO) 2P (O) N3) (0.74 ml, 3.5 mmol) in solution in DMF (2 ml), dropwise, and triethylamine (1 ml, 7.8 mmol) are successively added. After separation on silica gel (chloroform), 9-methoxy-l- (p-fluoro- phenyl)-2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one is recovered (750 mg, Y = 58%) NMR: 1H (CDCl3) : 2,71 (m, 4H), 2,89 (t, 2H), 3,82 (s, 3H), 4.19 (t, 2H), 6.77 (dd, 9 Hz and 3 Hz, 1H), 6.9-7.20 and 7.38 (3m, 7H) Mass spectrum: m/z: 362 (M+.), 319,253 Melting point: 191°C

Example14 : ANCARB07 C23H22N2O3M=374,43g.mol-1Formula: Structure : 9-methOxy-1-(p-methoxyphényl)-2,(p-methoxyphényl)-2, 3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one Preparation: A Bischler-Napieralski reaction, carried out on N- (2'- (5- methoxy-lH-3-indolyl) ethyl]-2- (p-methoxyphenyl) acetamide <BR> <BR> <BR> (800 mg, 2.1 mmol), results in the l- zyl)-6- methoxy-3, 4-dihydro-2-carboline, which is dissolved directly in anhydrous dimethylformamide (DMF) (10 ml).

This mixture is cooled to 0°C and acrylic acid (0.15 ml, 2.2 mmol), diphenylphosphoryl azide ((PhO) 2P (O) N3) (0.45 ml, 2 mmol) in solution in DMF (2 ml), dropwise, and triethylamine (0.53 ml, 4 mmol) are successively added. After separation on silica gel (chloroform), 9-methoxy-1- (p- <BR> <BR> <BR> methoxyphenyl)-2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin- 4-one is recovered (213 mg, Y = 27%).

NMR: 1H (CDCl3) : 2.67 (m, 4H), 2.90 (t, 6 Hz, 2H), 3.83 (s, 3H), 3.91 (s, 3H), 4.20 (t, 6 Hz, 2H), 6.77,6.88,7.04 and 7.33 (m, 7H) Mass spectrum: m/z: 374 (M+. 100), 359,253,187 Melting point: 154-155°C

Example 15 : DMACARB07 C24H25H3O2M=387,48g.mol-1Formula: Structure:

9-méthoxy-1-(p-dimAthylaminophényl)-2,(p-dimAthylaminophé nyl)-2, 7,12- hexahydroindolo [2,3-a] quinolizin-4-one Préparation: A Bischler-Napieralski reaction, carried out on N- (2'- (5- methoxy-lH-3-indolyl) ethyl]-2-(p-dimethylaminophenyl)- acetamide, results in the 1- (paradimethylaminobenzyl)-6-methoxy-3, 4- dihydro-2-carboline, which is dissolved directly in anhydrous dimethylformamide (DMF).

This mixture is cooled to 0°C and acrylic acid, diphenylphosphoryl azide ((PhO) 2P (O) N3) in solution in DMF, dropwise, and triethylamine are successively added. After separation on silica gel (chloroform), 9-methoxy-l- (p-di- 7,12-hexahydroindolo [2,3-a]- quinolizin-4-one is recovered.

NMR: 1H (CDCl3) : 2.69 (m, 4H), 2.89 (t, 2H), 3.04 (s, 6H), 3.82 (s, 3H), 4.20 (t, 2H), 6.80 (m, 3H), 6.89 (m, 2H), 7.25 (d, 2H), 7.29 (broad s, 1H) Mass spectrum: m/z: 387 (M+.), 194,142,134 (100)

Example 16 : PYRCARB07 Formula : C21Hl9N3°2 M = 345,40 g. mol-1 Structure : 9-methoxy-1- (pyrid-2'-yl)-2,3,4,6,7,12-hexahydroindolo [2,3- a] quinolizin-4-one Preparation : A Bischler-Napieralski reaction on the Nl- (2- (5-methoxy-lH- 3-indolyl) ethyl)-2- (pyrid-2'-yl)-acetamide (1.2g-3.9 mml) leads to 1- (2-pyridylmethyl)-6-methoxy-3,4-dihydro-2-carboline.

To a solution of 1- (2-pyridylmethyl)-6-methoxy-3,4-dihydro-2- carboline (760mg-2.6 mmol) in the DMF (9ml), acrylic acid is added (0.18 ml). Diphenylphosphorylazide (0.55 ml) is then added drip in solution in the DMF (3 ml), then the trietylamine (0.75 ml).

After separation on silica gel (Chloroform/methanol), 9- <BR> <BR> <BR> <BR> methoxyl-1- (pyrid-2'-yl)-2,3,4,6,7,12-hexahydroindolo [2,3- a] quinolizin-4-one (230mg-17.5%) is recovered.

NMR: 1H (CDCl3) : 2,73 (m, 2H); 2,85 (m, 2H); 2,94 (t, 2H); 3,18 (s, 3H), 4,24 (t, 2H); 6,85 (dd 2,4 et 9Hz, 1H); 6,93 (d 2,4Hz, 1H); 7,10 (d 9Hz, 1H); 7,30 (dd, 1H); 7,45 (d, 1H) ; 7,80 (dd, 1H); 8,75 (d, 1H) Mass Spectrum: m/z : 345 (M+', 100), 330,316,302 Melting point: > 260°C

Example 17: NPHCARB07 Formula : C23HlgN3°4 M = 389,41 g. mol-1 Structure : 9-methoxy-1-p-nitrophenyl-2, 3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one Preparation : A Bischler-Napieralski reaction on the Nl- (2- (5-methoxy-lH- <BR> <BR> <BR> 3-indolyl) ethyl)-2-p-nitrophenyl-acetamide (360mg-lmmol) leads to 1- (paranitrobenzyl)-6-methoxy-3,4-dihydro-2-carboline.

To a solution of 1- (paranitrobenzyl)-6-methoxy-3,4-dihydro-2- carboline in the DMF (10ml), acrylic acid (0.07ml) is added.

Diphenylphosphorylazide (0.21 ml) is then added drip and then triethylamine (0.26ml). After separation on silica gel (eluent AcOEt/EP-50/50), 9-methoxy-1-p-nitrophenyl- 2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one (163mg- 41) is recovered.

NMR: 1H (CDC13): 2,75 (m, 4H); 2,92 (t, 2H); 3,82 (s, 3H); 4 17 (t, 2H); 6,80 (dd 3 et 9 Hz, 1H); 6,90 (d 3 Hz, 1H) ; 6,92 (d 9Hz, 1H); 7,59 (d, 9Hz, 1H); 8,32 (, 9Hz, 1H).

Melting point: 138-140°C

Example 18 : TOLCARB07 Formula : C23H22N2°2 M = 358,43 g. mol-1 Structure :

9-méthOxy-1-p-tolyl-2,3,4,6,7,12-hexahydroindolo [2,3-a]<BR> <BR> <BR> <BR> <BR> <BR> quinolizin-4-one Preparation : A Bischler-Napieralski reaction on the Nl- (2- (5-methoxy-lH- 3-indolyl) ethyl)-2-p-tolyl-acetamide (2.12mg-6.6mmol) leads to 1- (paramethylbenzyl)-6-methoxy-3,4-dihydro-2-carboline.

To a solution of 1- (paramethylbenzyl)-6-methoxy-3,4-dihydro-2- carboline in the DMF (15ml), acrylic acid (0.46ml) is added.

Diphenylphosphorylazide (1.4 ml) is then added drip and then triethylamine (1.75ml). After separation on silica gel (eluent Chloroform/methanol), 9-methoxy-1-p-tolyl-2,3,4,6, 7,12-hexahydroindolo [2,3-a] quinolizin-4-one is recovered.

NMR: 1H (CDCl3) : 2,46 (s, 3H); 2, 73 (m, 4H); 2,88 (t, 2H); 3,82 (s, 3H); 4,20 (t, 2H) ; 6, 75 (dd 2 et 9Hz, 1H); 6,84 (d 9Hz, 1H); 6,88 (d 2Hz, 1H); 7,03 (s large, 1H); 7,30 (m, 4H) Melting point: 198°C

Example 19 : PHDHCARB07 Formula: C22H22N2°2 M = 346.42g. mol-1 Structure : 9-methoxy-1-phenyl-1,2,3,4,6,7,12,12b-octahydroindolo [2,3- a] quinolizin-4-one Preparation: Sodium bicarbonate (900 mg) and palladium on charcoal are successively added to a solution of 9-methoxy-1-phenyl- 2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one (2.4 g) in mixture ethyl acetate-ethanol 1/1 (160 mL), and the mixture is stirred overnight under a hydrogen atmosphere.

After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate. 9-methoxy-1- phenyl-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizin-4 -one is thus recovered (2.3 g, yield = 95%).

NMR: 1H (CDCl3) : 2.05 (m, 1H), 2.28 (m, 1H), 2.47 (m, 2H), 2.74 (m, 1H), 2.97 (m, 2H), 3.81 (m + s, 4H), 5.18 (m, 2H), 6.71 (dd, 2.4 Hz and 9 Hz, 1H), 6.89 (d, 2.4 Hz, 1H), 6.93 (d, 9Hz, 1H), 7.25 (m, 5H), 7.5 (broad s, 1H).

Mass spectrum: m/z : 346 (M+.), 242,200.

Melting point: 162°C

Example20 : FPHDHCARB07<BR> <BR> <BR> <BR> Formula: C22H21N2FO2 M = 364.41 g mol 1 Structure: 9-methoxy-l- (parafluorophenyl)-1,2,3,4,6,7,12,12 boctahydroindolo [2,3-a] quinolizin-4-one Preparation: Sodium bicarbonate (50 mg) and palladium on charcoal are successively added to a solution of 9-methoxy-1- (parafluorophenyl)-2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one (64 mg) in ethyl acetate, and the mixture is stirred overnight under a hydrogen atmosphere. After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate. 9-methoxy-l- (parafluorophenyl)-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizin-4-one is thus recovered (21 mg, yield = 33%).

NMR: 1H (CDC13) : 2.05 (m, 1H), 2.40 (m, 3H), 2.74 (m, 1H), 2.90 (m, 2H), 3.80 (ls+lm, 4H), 5.19 (m, 2H), 6.71 (dd, 2.4 and 9 Hz, 1H), 6.92 (m, 3H), 6.97 (d, 8.7 Hz, 1H), 7.18 (m, 2H), 7.52 (broad s, 1H).

Mass spectrum: m/z : 364 (M+.), 242,200.

Melting point: 162-166°C

Example 21 : PHDHNAPH7 Formula: C24H23NO2 M = 357.45g. mol-1 Structure: 3-methoxy-11-phenyl-5,8,9,10,11,11a-hexahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-one Preparation: Sodium bicarbonate (20 mg) and palladium-on charcoal are successively added to a solution of 3-methoxy-11-phenyl- 5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8- one (30 mg) in ethyl acetate (15 mL) and the mixture is stirred overnight under a hydrogen atmosphere. After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate. 3-methoxy-11-phenyl- 5,8,9,10,11,11a-hexahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-one is thus recovered (25mg, yield = 83-t).

NMR: 1H (CDCl3) : 2.14 (m, 1H), 2.48 to 3.16 (m, 5H), 3.13 (d, 15.6 Hz, 1H), 3.94 (m + s, 4H), 5.16 (ddd, 12.8 Hz, 5.1 Hz and 2.1 Hz, 1H), 5.28 (d, 4.4 Hz, 1H), 6.89 to 7.13 (m, 8H), 7.55 (d, 8.5Hz, 1H), 7.62 (d, 9.6Hz, 1H).

Mass spectrum: m/z : 357 (M+.), 329,253,211 Melting point: 175°C

Example 22 : ETDHNAPH7 Formula: C2oH23NO2 M = 309. 40g. mol 1 Structure : 11-ethyl-3-methoxy-5,8,9,10,11,11a-hexahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-one Preparation: Sodium bicarbonate (30 mg) and palladium on charcoal are successively added to a solution of 11-ethyl-3-methoxy- 5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2, 1-a] isoquinolin-8- one (74 mg) in ethyl acetate (25 mL) and the mixture is stirred overnight under a hydrogen atmosphere. After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate. 3-methoxy-11-ethyl- 5,8,9,10,11,11a-hexahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-one is thus recovered (50 mg, yield = 67z).

NMR: 1H (CDCl3) : 0.73 (t, 6.8Hz, 3H), 1.08 (m, 2H), 2.05 (m, 2H), 2.30 to 3.05 (m, 5H), 3.16 (d, 16.0 Hz, 1H), 3.96 (s, 3H), 5.02 (broad s, 1H), 5,17 (ddd, 12.4 Hz, 4.4 Hz and 16 Hz, 1H), 7.12 to 7.21 (m, 3H), 7.67 (d, 8.6Hz, 1H), 7.73 (d, 8.6Hz, 1H).

Mass spectrum: m/z : 309 (M+.), 253,225,211.

Melting point: 206°C

Example 23: ANIDHCARB07 Formula: C22H2gNg02 M = 361.45g. mol 1 Structure : 9-methoxy-1-paraaminophenyl-1,2,3,4,6,7,12,12b octahydroindolo [2,3-a] quinolizin-4-one Preparation: Sodium bicarbonate (50 mg) and palladium on charcoal are successively added to a solution of 9-methoxy-1- paranitrophenyl-2,3,4,6,7,12-hexahydroindolo[2,3-a] quinolizin-4-one (91 mg) in ethyl acetate and the mixture is stirred overnight under a hydrogen atmosphere. After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate. 9-methoxy-1- 7,12,12b-octahydroindolo [2,3-a] quinolizin-4-one is thus recovered (37 mg, yield 44%).

NMR: 1H (CDCl3) : 1.95 (m, 1H), 2.28 (m, 1H), 2.40 (m, 2H), 2.90 (m, 2H), 3.70 (m, 1H), 3.80 (m, 3H), 4.20 (broad s, 2H), 5.20 (m, 2H), 6.49 (d, 8.3 Hz, 1H), 6.65 (dd, 8.7 Hz, 2.4 Hz, 1H), 6.83 (d, 2.4 Hz, 1H), 6.90 (d, 8.4 Hz, 1H), 6.95 (d, 8.7 Hz, 1H).

Mass spectrum: m/z : 361 (M+.), 242,200,181.

Melting point: 165-166°C

Example 24 : PHDHCARB07S Formula: C22H22N2OS M = 362.48g. mol-1 Structure : 9-methoxy-1-phenyl-1,2,3,4,6, 7,12,12b-octahydroindolo [2,3-a] quinolizin-4-thione Preparation: To 9-methoxy-1-phenyl-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizin-4-one (520 mg) in anhydrous toluene (50mL) we added, at 110°C, Lawesson reactif (640 mg), in small quantities. After refluxing for 30 min and evaporation of toluene, the product is isolated by column chromatography over SiO (Eluent chloroform/methanol, 99/1), we obtained the 9-methoxy-1-phenyl-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizine-4-thione (160 mg, yield 30%).

NMR: 1H (CDCl3) : 1.95 (m, 1H), 2.20 (m, 1H), 2.72 (dd, 12 Hz, j = 2.8 Hz, 1H), 2.97 (m, 3H), 3.41 (m, 1H), 3.80 (m + s, 4H), 5.33 (d, j = 4 Hz, 1H), 5.93 (dd, 5.1 Hz and 1.3 Hz, 1H), 6.71 (dd, 8. 7 Hz and 2,4 Hz, 1H), 6.83 (d, 2.4Hz, 1H), 6.94 (d, 8.7 Hz, 1H), 7.20 (m, 5H).

Melting point: 242-244°C

Example 25 : ETNAPH7S Formula: C2pH21NOS M = 323.45g. mol 1 Structure:

11-ethyl-3-methoxy-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-thione Preparation: To ll-ethyl-3-methoxy-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-one (45 mg) in anhydrous toluene (lOmL) we added, at 110°C, Lawesson reactif (70mmol), in small quantities. After refluxing for 30 min and evaporation of toluene the product is isolated by column chromatography over SiO, (Eluent dichloromethane), we obtained the 11-ethyl-3-methoxy-5,8,9,10-tetrahydro-6H- benzo [f] pyrido [2,1-a] isoquinolin-8-thione (32 mg, yield 70).

NMR: 1H (CDCl3) : 1.14 (t, 7.3 Hz, 3H), 2.35 (m, 4H), 3.13 (t, 7.7 Hz, 2H), 3.. 25 (t, 6 Hz, 2H), 3.99 (s, 3H), 4.27 (t, 6 Hz, 2H), 7.19 (dd, 8.5 and 2.4 Hz, 1H), 7.28 (m, 2H), 7.67 (d, 8.5 Hz, 1H), 7.76 (d, 8.9 Hz, 1H).

Melting point: 164-166°C

Example 26 : PHNAPH7S Formula: C24H21NOS M = 371.49g. mol-1 Structure :

3-methoxy-11-phenyl-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-thione Preparation: To 3-methoxy-11-phenyl-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-one (50 mg) in anhydrous toluene (lOmL) we added, at 110°C, Lawesson reactif (66 mg), in small quantities. After refluxing for 30 min and evaporation of toluene the product is isolated by column chromatography over Si0= (dichloromethane), we obtained the 3-methoxy-11-phenyl-5,8,9,10-tetrahydro-6H-benzo [f] pyrido [2,1-a] isoquinolin-8-thione (37mg, yield 74i).

NMR: 1H (CDCl3) : 2.71 (t, 7.8 Hz, 1H), 3.36 (m, 4H), 3.97 (s, 3H), 4.36 (t, 6.2 Hz, 1H), 6.68 (d, 8.5 Hz, 1H), 7.18 (m, 8H), 7.63 (d, 8.7 Hz, 1H).

Melting point: 136°C

Example27 : ETDHCARB07S C18H22N2OSM=314,44g.mol-1Formula: Structure :

1-ethyl-9-methoxy-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-<BR> <BR> <BR> a] quinolizine-4-thione Preparation : To 1-ethyl-9-methoxy-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizine-4-one (262 mg) in anhydrous toluene (20 mL) we added, at 110°C, Lawesson reactif (208 mg) in small quantities. After refluxing for 30 min and evaporation of toluene, the product is isolated by column chromatography over SiO2 (Eluent chloroform/methanol, 99/1) we obtained the 1-ethyl-9-methoxy-1,2,3,4,6,7,12,12b-octahydroindolo [2,3- a] quinolizine-4-thione (30 mg, yield 11.5",-).

NMR: 1H (CDC13) : 0.81 (t, 3H), 1.06 (m, 2H), 1.90 (m, 2H) 2.30 (m, 1H), 2.80 (m, 5H), 3.85 (s, 3H), 4.94 (s, 1H), 6.08 (m, 1H), 6.87 (dd, 2.4 and 8.7Hz, 1H), 6.96 (d, 2. 4Hz, 1H), 7.26 (d, 8.7Hz, 1H), 7.82 (s large, 1H).

Melting point: 124°C

Example 28 : C02ETDHCARB07 Formula : C1gH22N204 M = 342,39 g. mol-1 Structure: 1-carbethoxy-9-methoxy-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a]quinolizin-4-one Preparation: Sodium bicarbonate (10 mg) and palladium on charcoal are successively added to a solution of 1-carbethoxy-9-methoxy- 2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one (50 mg) in ethyl acetate, and the mixture is stirred overnight under hydrogen atmosphere. After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate. 1-carbethoxy-9-methoxy-1,2,3,4,6,7,12,12b-octa- hydroindolo [2,3-a] quinolizin-4-one is thus recovered (30 mg, yield = 60%).

NMR: 1H (CDCl3) : 1. 02 (t, 3H), 2.15 (m, 2H), 2.85 (m, 8H), 3.35 (m, 1H), 3.85 (s, 3H), 4.05 (t, 2H), 5.08 (m, 2H), 6.82 (dd, 8.7 and 2.4Hz, 1H), 6.92 (d, 2.4Hz, 1H), 7.21 (d, 8.7Hz, 1H), 8.24 (broad s, 1H).

Mass spectrum: m/z : 342 (M+.), 286,269,240,199.

Melting point: 213°C

Example 29 : TOLDHCARB07 Formula: C23H24N202 M = 360.46g. mol 1 Structure :

9-methoxy-1-paratolyl-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizin-4-one Preparation : Sodium bicarbonate (50 mg) and palladium on charcoal are successively added to a solution of 9-methoxy-1-paratolyl- 2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one (55 mg) in ethyl acetate and the mixture is stirred overnight under a hydrogen atmosphere. After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate, 9-methoxy-1-paratolyl-1,2,3,4,6,7,12,12b-Octahydro- indolo [2,3-a] quinolizin-4-one is thus recovered (32 mg, yield 58%).

NMR: 1H (CDCl3) : 2.04 (m, 1H), 2.23 (m, 1H), 2.29 (s, 3H), 2.46 (m, 2H), 2.74 (m, 1H), 2.94 (m, 2H), 3.70 (m, 1H), 3.80 (s, 3H), 5.20 (m, 1H), 5.24 (d, 1H), 6.71 (dd, 2.4Hz and 8,7Hz, 1H), 6.87 (d, 2.4Hz, 1H), 6.93 (d, 8.7Hz, 1H), 7.08 (d, 8.0Hz, 2H), 7.16 (d, 8. OHz, 2H), 7.2 (1H, NH).

Example 30 : ANDHCARB07 Formula: C23H24N203 M = 376.46g. mol-1 Structure :

9-methoxy-1-paraanisyl-1,2,3,4,6,7,12,12b-octahydroindolo [2,3-a] quinolizin-9-one Preparation : Sodium bicarbonate (50 mg) and palladium on charcoal are successively added to a solution of 9-methoxy-1-paraanisyl- 2,3,4,6,7,12-hexahydroindolo [2,3-a] quinolizin-4-one (43 mg) in ethyl acetate and the mixture is stirred overnight under a hydrogen atmosphere. After filtration and evaporation of the solvent, the crude product is recrystallized from ethyl acetate, 9-methoxy-1-paraanisyl-1,2,3,4,6,7,12,12b-octa- hydroindolo [2,3-a] quinolizin-4-one is thus recovered (24 mg, yield 56%).

NMR: 1H (CDCl3) : 2.05 (m, 1H), 2.25 (m, 1H), 2.46 (m, 2H), 2.74 (d, 1H), 2.54 (m, 2H), 3.68 (m, 1H), 3.75 (s, 3H), 3.80 (s, 3H), 5.14 (m, 1H), 5.23 (d, 5.5Hz, 1H), 6.72 (dd, 2.4 and 8.7Hz, 1H), 6.82 (d, 8.7Hz, 2H), 6.87 (d, 2.4Hz, 1H), 6.96 (d, 8.7Hz, 1H), 7,17 (d, 8.7Hz, 2H).

Melting point: 242°C Example 31 : DIETCARB07 Formula : C2oH26N202 M = 326.43g. mol~ Structure :

7,12,12b-octahydroindolo [2,3-a] quinolizine-4-one Preparation : 5-methoxytryptamine (494 mg-2,59 mmol) and ethyl (-4-ethyl -9-formyl) caproate (522 mg-2,61 mmol) are mixed in commercial toluene (27 mL) in a 50 mL flask.

The medium is then heated to the toluene reflux during 2 hours. After cooling, the toluene is evaporated under reduced pressure and acetic acid (1 mL) is added.

The medium is then heated to the acetic acid reflux during 2 hours. After cooling, water (25 mL) is added and a solid precipitates. This solid is diluted with ethyl acetate and washed with water. The resulting organic phase is dried over magnesium sulfate and the solvent is evaporated under reduced pressure.

After separation on silica gel (chloroform/methanol- 97.5/2.5 eluent), 7, 12,12b-octahydroindolo [2,3-a] quinolizine-4-one-is obtained (200 mg, yield 23%).

NMR: 1H (CDCl3) : 0.73 (t, 7.5Hz, 3H), 1.02 (q, 7.5Hz, 1H), 1.18 (t, 7.5Hz, 3H) 1.50 (q 7.5Hz, lH), 1.60 (m, 1H), 1.80 (m, 4H), 2.49 (m, 1H), 2.76 (m, 3H), 3.85 (s, 3H), 4.83 (s, ils), 5.17 (m, 1H), 6.84 (dd, 2.3 and 8.7Hz, 1H), 6.85 (d, 2.3Hz, 1H, 7.24 (d, 8.7Hz, 1H), 7.77 (s broad, 1H).

Melting point: 229°C

Example 32 : ETCARB07TL Formula: C21H24N2O4 M = 368.43g. mol-1 Structure : (6S)-6-carbethoxy-9-methoxy-1-ethyl-2,3,4,6,7,12- hexahydroindolo [2,3-a] quinolizin-4-one Preparation : A Bischler-Napieralski reaction on Nl- (butyryl)-5-methoxy- (L)-tryptophan-ethyl ester (2.1 g) lead to the (3S)-l-propyl- 3-carbethoxy-6-methoxy-3, 4-dihydra-2-carboline (1. 5 g). To a solution of (3S)-l-propyl-3-carbethoxy-6-methoxy-3,4-dihydro-2- carboline (1.5 g) in DMF (20 mL) was added dropwise successively acrylic acid (0.35 mL), diphenylphosphoryl azide (1 mL) and triethylamine (2 mL). After separation on silica gel (eluent-ethyl acetate/petroleum ether-30/70), <BR> <BR> <BR> (6S)-6-carbethoxy-9-methoxy-l-ethyl-2,3,4,6,7,12-hexahydro- indolo [2,3-a] quinolizin-4-one is recovered (400 mg-17%) NMR: 1H (CDC13): 1-1 (t, 3H), 1.25 (t, 3H), 2.3 (m, 1H), 2.62 (m, 5H), 3.08 (dd, 6 and 16 Hz, 1H), 3.53 (d, 16Hz, 1H), 3.85 (s, 3H), 4.03 (m, 2H), 6.00 (d, 6Hz, 1H), 6.85 (dd, 2.3 and <BR> <BR> <BR> 8.7 Hz, 1H), 6.94 (d, 2.3Hz, 1H), 7.24 (d, 8.7Hz, 1H), 8.13 ( s broad, 1 H) Melting point: 192°C CLAIMS