Title:
HETEROLOGOUS PRODUCTION OF POLYKETIDES
Document Type and Number:
WIPO Patent Application WO2001083800
Kind Code:
A3
Abstract:
Recombinant Myxococcus host cells can be used to produce polyketides, including epothilone and epothilone analogs that can be purified from the fermentation broth and crystallized.
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Inventors:
ARSLANIAN ROBERT L (US)
ASHLEY GARY (US)
FRYKMAN SCOTT (US)
JULIEN BRYAN (US)
KATZ LEONARD (US)
KHOSLA CHAITAN (US)
LAU JANICE (US)
LICARI PETER J (US)
REGENTIN RIKA (US)
SANTI DANIEL (US)
TANG LI (US)
ASHLEY GARY (US)
FRYKMAN SCOTT (US)
JULIEN BRYAN (US)
KATZ LEONARD (US)
KHOSLA CHAITAN (US)
LAU JANICE (US)
LICARI PETER J (US)
REGENTIN RIKA (US)
SANTI DANIEL (US)
TANG LI (US)
Application Number:
PCT/US2001/013793
Publication Date:
April 10, 2003
Filing Date:
April 26, 2001
Export Citation:
Assignee:
KOSAN BIOSCIENCES INC (US)
ARSLANIAN ROBERT L (US)
ASHLEY GARY (US)
FRYKMAN SCOTT (US)
JULIEN BRYAN (US)
KATZ LEONARD (US)
KHOSLA CHAITAN (US)
LAU JANICE (US)
LICARI PETER J (US)
REGENTIN RIKA (US)
SANTI DANIEL (US)
TANG LI (US)
ARSLANIAN ROBERT L (US)
ASHLEY GARY (US)
FRYKMAN SCOTT (US)
JULIEN BRYAN (US)
KATZ LEONARD (US)
KHOSLA CHAITAN (US)
LAU JANICE (US)
LICARI PETER J (US)
REGENTIN RIKA (US)
SANTI DANIEL (US)
TANG LI (US)
International Classes:
C07D313/00; C07D405/06; C07D413/06; C07D417/06; C07D491/04; C12N1/20; C12N1/21; C12N9/00; C12N15/52; C12N15/74; C12P17/16; C12P17/18; C12R1/01; (IPC1-7): C12N15/52; C12N15/74; C12N9/00; C12N1/21; C12P17/16; C12P17/18; C07D313/00; C07D413/06; C07D417/06; C07D493/04; C07D493/06
Domestic Patent References:
| WO2000022139A2 | 2000-04-20 | |||
| WO1999066028A2 | 1999-12-23 | |||
| WO1998022461A1 | 1998-05-28 | |||
| WO2000031247A2 | 2000-06-02 |
Other References:
TANG L ET AL.: "Cloning and heterologous expression of the epothilone gene cluster", SCIENCE, vol. 287, no. 5453, 2000, pages 640 - 642, XP000910114, ISSN: 0036-8075
BEYER S ET AL.: "Metabolic diversity in myxobacteria: identification of the myxalamid and the stigmatellin biosynthetic gene cluster of Stigmatella aurantiaca Sg a15 and a combined polyketide-(poly)peptide gene cluster from the epothilone producing strain Sorangium cellulosum So ce90", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, vol. 1445, no. 2, 14 May 1999 (1999-05-14), pages 185 - 195, XP004275376, ISSN: 0167-4781
SILAKOWSKI B ET AL.: "New lessons for combinatorial biosynthesis from myxobacteria: The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 52, 24 December 1999 (1999-12-24), pages 37391 - 37399, XP002199229, ISSN: 0021-9258
PAITAN Y ET AL.: "The first gene in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus codes for a unique PKS module coupled to a peptide synthetase", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 286, no. 1, 1999, pages 465 - 474, XP000920956, ISSN: 0022-2836
SHIMKETS L J: "Industrial relevance and genetic analysis of myxobacteria.", INDUSTRIAL MICROORGANISMS: BASIC AND APPLIED MOLECULAR GENETICS., 1993, Fifth ASM;Bloomington, Indiana, USA; October 11-15, 1992, American Society for Microbiology (ASM) Books Division, 1325 Massachusetts Ave. NW, Washington, DC 20005-4171, USA, pages 85 - 96, XP008003384, ISBN: 1-55581-063-2
NICOLAOU K C ET AL.: "Chemical Biology of Epothilones", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 37, no. 15, August 1998 (1998-08-01), pages 2014 - 2045, XP002131418, ISSN: 0570-0833
BRETSCHER A P ET AL.: "Nutrition of Myxococcus xanthus, a fruiting Myxobacterium", JOURNAL OF BACTERIOLOGY, vol. 133, no. 2, 1978, pages 763 - 768, XP001010255, ISSN: 0021-9193
REGENTIN R ET AL.: "Development of a cost effective epothilone D process in Myxococcus xanthus.", ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, vol. 221, no. 1-2, 1 April 2001 (2001-04-01), 221st National Meeting of the American Chemical Society;San Diego, California, USA; April 01-05, 2001, pages BIOT 61, XP008003385, ISSN: 0065-7727
PFEIFER B A ET AL.: "Biosynthesis of polyketides in heterologous hosts", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 65, no. 1, March 2001 (2001-03-01), pages 106 - 118, XP000997626, ISSN: 1092-2172
FRYKMAN S ET AL.: "Modulation of epothilone analog production through media design.", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 28, no. 1, January 2002 (2002-01-01), pages 17 - 20, XP008003393, ISSN: 1367-5435
ARSLANIAN R L ET AL.: "Large-scale isolation and crystallization of epothilone D from Myxococcus xanthus cultures.", JOURNAL OF NATURAL PRODUCTS, vol. 65, no. 4, April 2002 (2002-04-01), pages 570 - 572, XP001074701, ISSN: 0163-3864
LAU J ET AL.: "Optimizing the heterologous production of epothilone D in Myxococcus xanthus.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 78, no. 3, 5 May 2002 (2002-05-05), pages 280 - 288, XP002199234, ISSN: 0006-3592
BEYER S ET AL.: "Metabolic diversity in myxobacteria: identification of the myxalamid and the stigmatellin biosynthetic gene cluster of Stigmatella aurantiaca Sg a15 and a combined polyketide-(poly)peptide gene cluster from the epothilone producing strain Sorangium cellulosum So ce90", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, vol. 1445, no. 2, 14 May 1999 (1999-05-14), pages 185 - 195, XP004275376, ISSN: 0167-4781
SILAKOWSKI B ET AL.: "New lessons for combinatorial biosynthesis from myxobacteria: The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 52, 24 December 1999 (1999-12-24), pages 37391 - 37399, XP002199229, ISSN: 0021-9258
PAITAN Y ET AL.: "The first gene in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus codes for a unique PKS module coupled to a peptide synthetase", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 286, no. 1, 1999, pages 465 - 474, XP000920956, ISSN: 0022-2836
SHIMKETS L J: "Industrial relevance and genetic analysis of myxobacteria.", INDUSTRIAL MICROORGANISMS: BASIC AND APPLIED MOLECULAR GENETICS., 1993, Fifth ASM;Bloomington, Indiana, USA; October 11-15, 1992, American Society for Microbiology (ASM) Books Division, 1325 Massachusetts Ave. NW, Washington, DC 20005-4171, USA, pages 85 - 96, XP008003384, ISBN: 1-55581-063-2
NICOLAOU K C ET AL.: "Chemical Biology of Epothilones", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 37, no. 15, August 1998 (1998-08-01), pages 2014 - 2045, XP002131418, ISSN: 0570-0833
BRETSCHER A P ET AL.: "Nutrition of Myxococcus xanthus, a fruiting Myxobacterium", JOURNAL OF BACTERIOLOGY, vol. 133, no. 2, 1978, pages 763 - 768, XP001010255, ISSN: 0021-9193
REGENTIN R ET AL.: "Development of a cost effective epothilone D process in Myxococcus xanthus.", ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, vol. 221, no. 1-2, 1 April 2001 (2001-04-01), 221st National Meeting of the American Chemical Society;San Diego, California, USA; April 01-05, 2001, pages BIOT 61, XP008003385, ISSN: 0065-7727
PFEIFER B A ET AL.: "Biosynthesis of polyketides in heterologous hosts", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 65, no. 1, March 2001 (2001-03-01), pages 106 - 118, XP000997626, ISSN: 1092-2172
FRYKMAN S ET AL.: "Modulation of epothilone analog production through media design.", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 28, no. 1, January 2002 (2002-01-01), pages 17 - 20, XP008003393, ISSN: 1367-5435
ARSLANIAN R L ET AL.: "Large-scale isolation and crystallization of epothilone D from Myxococcus xanthus cultures.", JOURNAL OF NATURAL PRODUCTS, vol. 65, no. 4, April 2002 (2002-04-01), pages 570 - 572, XP001074701, ISSN: 0163-3864
LAU J ET AL.: "Optimizing the heterologous production of epothilone D in Myxococcus xanthus.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 78, no. 3, 5 May 2002 (2002-05-05), pages 280 - 288, XP002199234, ISSN: 0006-3592
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