HIGH-EFFICIENCY SYNTHESIS AND HIGH-PURITY HYALURONIC ACID, AND RECOMBINANT CORYNEBACTERIUM GLUTAMICUM FOR OLIGOSACCHARIDE THEREOF

Title:

HIGH-EFFICIENCY SYNTHESIS AND HIGH-PURITY HYALURONIC ACID, AND RECOMBINANT CORYNEBACTERIUM GLUTAMICUM FOR OLIGOSACCHARIDE THEREOF

Document Type and Number:

WIPO Patent Application WO/2021/077580

Kind Code:

A1

Abstract:

Disclosed is a high-efficiency synthesis and high-purity hyaluronic acid, and a recombinant corynebacterium glutamicum for an oligosaccharide thereof, relating to the technical field of bio-engineering. The yield of hyaluronic acid produced by the constructed recombinant corynebacterium glutamicum reaches 40 g/L, and the purity of the crude product reaches 95%. By means of a hyaluronic acid hydrolase from an external source and optimizing fermentation conditions, hyaluronic acid oligosaccharide of a specific molecular weight can be obtained, and further increasing of hyaluronic acid yield can be achieved, causing the yield to reach 72 g/L.

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Inventors:

KANG ZHEN (CN)
CHEN JIAN (CN)
HU LITAO (CN)
DU GUOCHENG (CN)
WANG YANG (CN)
LI JIANGHUA (CN)
LI JIALIAN (CN)
ZHANG TIANMENG (CN)

Application Number:

PCT/CN2019/126013

Publication Date:

April 29, 2021

Filing Date:

December 17, 2019

Export Citation:

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Assignee:

BLOOMAGE BIOTECHNOLOGY CORP LTD (CN)
UNIV JIANGNAN (CN)

International Classes:

C12N1/21; C12N15/77; C12P19/26; C12R1/15

Foreign References:

CN103937734A	2014-07-23
CN101709285A	2010-05-19
CN106190939A	2016-12-07
CN107354119A	2017-11-17
CN103597088A	2014-02-19
CN108251346A	2018-07-06

Other References:

CHENG FANGYU, LUOZHONG SIJIN, GUO ZHIGANG, YU HUIMIN, STEPHANOPOULOS GREGORY: "Enhanced Biosynthesis of Hyaluronic Acid Using Engineered Corynebacterium glutamicum Via Metabolic Pathway Regulation", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 12, no. 10, 1 October 2017 (2017-10-01), DE, pages 1700191, XP055803709, ISSN: 1860-6768, DOI: 10.1002/biot.201700191
See also references of EP 4050096A4

Attorney, Agent or Firm:

ZHONGZI LAW OFFICE (CN)

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