Title:
HIGH-THROUGHPUT GENE-EDITING TECHNIQUE
Document Type and Number:
WIPO Patent Application WO/2020/179931
Kind Code:
A1
Abstract:
One of the purposes of the present invention is to provide a method for quickly producing a gene-edited tissue stem cell in an undifferentiated state. Another one of the purposes of the present invention is to provide a therapeutic and/or prophylactic method for disease through usage of a gene-edited tissue stem cell in an undifferentiated state. According to the present invention, a gene-edited tissue stem cell is produced by a method comprising a step for editing the gene sequence of a target gene in tissue stem cells, and a step for screening the tissue stem cells having an edited gene sequence, and optionally, further comprising a step for adding a tag to the gene sequence of the target gene and/or a step for activating gene expression of the target gene in the tissue stem cells. The tissue stem cell thus produced is used in therapy/prevention of diseases.
Inventors:
ASAHARA HIROSHI (JP)
KURIMOTO RYOTA (JP)
MATSUSHIMA TAKAHIDE (JP)
TSUTSUMI HIROKI (JP)
KURIMOTO RYOTA (JP)
MATSUSHIMA TAKAHIDE (JP)
TSUTSUMI HIROKI (JP)
Application Number:
PCT/JP2020/009987
Publication Date:
September 10, 2020
Filing Date:
March 09, 2020
Export Citation:
Assignee:
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
International Classes:
A61P7/00; A61K35/12; A61K35/545; A61L27/38; A61P37/02; C12N5/074; C12N5/10; C12N15/09
Domestic Patent References:
| WO2016044416A1 | 2016-03-24 | |||
| WO2018170239A1 | 2018-09-20 | |||
| WO2019239361A1 | 2019-12-19 |
Other References:
KONERMANN, S. ET AL.: "Genome-scale transcriptional activation by an engineered CRISPR- Cas9 complex", NATURE, vol. 517, 2015, pages 583 - 588, XP055585957, DOI: 10.1038/nature14136
MADSEN, R. R. ET AL.: "Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells", WELLCOME OPEN RESEARCH, vol. 4, no. 37, 2019
MADSEN, R. R. ET AL.: "Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells", WELLCOME OPEN RESEARCH, vol. 4, no. 37, 2019
Attorney, Agent or Firm:
ITO Katsuhiro et al. (JP)
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